AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32612


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32612.t1 MTR_3g107610 97.076 171 4 1 1 171 1 170 2.76e-108 306
MS.gene32612.t1 MTR_3g107610 90.789 152 12 2 1 152 1 150 2.53e-85 248
MS.gene32612.t1 MTR_4g011190 61.111 162 47 4 1 162 1 146 2.09e-55 172
MS.gene32612.t1 MTR_4g113840 43.030 165 51 6 5 161 6 135 4.59e-33 114
MS.gene32612.t1 MTR_2g054750 36.943 157 58 3 5 160 6 122 8.07e-31 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32612.t1 AT3G15680 60.819 171 58 5 1 169 1 164 4.32e-61 187
MS.gene32612.t1 AT3G15680 60.819 171 58 5 1 169 1 164 4.32e-61 187
MS.gene32612.t1 AT5G25490 62.791 172 50 5 1 164 1 166 1.37e-55 173
MS.gene32612.t1 AT5G25490 62.791 172 50 5 1 164 1 166 1.37e-55 173
MS.gene32612.t1 AT2G26695 40.764 157 65 5 5 161 6 134 1.99e-32 113
MS.gene32612.t1 AT2G26695 40.764 157 65 5 5 161 6 134 1.99e-32 113
MS.gene32612.t1 AT2G17975 30.769 169 80 6 3 157 5 150 4.56e-16 73.9

Find 71 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGCGGCGCCGTTGATTT+TGG 0.178200 3.1:-85134761 MS.gene32612:CDS
TTGAGATCACCATACGGTTT+TGG 0.292602 3.1:-85134539 MS.gene32612:CDS
GGGGAGTCAAGAATGATTTC+TGG 0.322815 3.1:-85134785 MS.gene32612:CDS
TTGAGAAGTTCACGATAAAT+AGG 0.332528 3.1:+85134064 None:intergenic
TCGGTAGCATTCCATTCTAT+TGG 0.379779 3.1:+85134124 None:intergenic
GGCTCCTTTCTTGGTGGAAG+AGG 0.386495 3.1:-85134737 MS.gene32612:CDS
GAGAAGGTATCAATATCCTT+AGG 0.387497 3.1:+85134590 None:intergenic
ACTCGCCCCGGCTGGAAATC+CGG 0.405258 3.1:-85134488 MS.gene32612:CDS
GTGCCTCCACTCGCCCCGGC+TGG 0.416254 3.1:-85134496 MS.gene32612:CDS
GGGCCGGTGGTGAAAGGAAA+AGG 0.421977 3.1:+85134704 None:intergenic
CAGTCACCAGGACGGACATC+AGG 0.429559 3.1:+85134683 None:intergenic
CTGATGTCCGTCCTGGTGAC+TGG 0.431260 3.1:-85134682 MS.gene32612:CDS
AGATGAGCAGACCAGGAGAT+TGG 0.434758 3.1:-85134865 MS.gene32612:CDS
GTCCTGCAGTTCCAATCTCC+TGG 0.444104 3.1:+85134854 None:intergenic
CTCTTCTTTGAAAGTTGAGG+TGG 0.445847 3.1:+85134825 None:intergenic
GGCACCACATTTGAAGCAGC+TGG 0.455963 3.1:+85134613 None:intergenic
GGAGGAGGCAGCGCCGGTGG+TGG 0.457413 3.1:-85134518 MS.gene32612:CDS
TTTGAAGCAGCTGGAGCGGC+TGG 0.457465 3.1:+85134622 None:intergenic
CGCTCCAGCTGCTTCAAATG+TGG 0.474359 3.1:-85134617 MS.gene32612:CDS
CTTCAAATGTGGTGCCCCTA+AGG 0.478199 3.1:-85134606 MS.gene32612:CDS
CAGTCACCGGATTTCCAGCC+GGG 0.479965 3.1:+85134482 None:intergenic
CCGGCTGGAAATCCGGTGAC+TGG 0.485260 3.1:-85134481 MS.gene32612:CDS
AGAGCCTCTTCCACCAAGAA+AGG 0.496788 3.1:+85134733 None:intergenic
GCATAACTTCGCCAATAGAA+TGG 0.497407 3.1:-85134135 MS.gene32612:CDS
CTTCCAGTACTAGATTCCCT+TGG 0.497781 3.1:+85134092 None:intergenic
ATTCTCTTCTTTGAAAGTTG+AGG 0.498262 3.1:+85134822 None:intergenic
TTTGGTGGCTCCTTTCTTGG+TGG 0.500076 3.1:-85134743 MS.gene32612:CDS
AGCAGAAAGATGAGCAGACC+AGG 0.504369 3.1:-85134872 None:intergenic
CACATTTGAAGCAGCTGGAG+CGG 0.504933 3.1:+85134618 None:intergenic
GGCCGGTGGTGAAAGGAAAA+GGG 0.509497 3.1:+85134705 None:intergenic
AGAAGGTATCAATATCCTTA+GGG 0.509984 3.1:+85134591 None:intergenic
GACCAGGAGATTGGAACTGC+AGG 0.511105 3.1:-85134856 MS.gene32612:CDS
GCTACCGATGCAACGGTCCA+AGG 0.513927 3.1:-85134109 MS.gene32612:CDS
GGATTTCCAGCCGGGGCGAG+TGG 0.514801 3.1:+85134490 None:intergenic
AGAATGATTTCTGGCGGCGG+CGG 0.516918 3.1:-85134776 MS.gene32612:CDS
GCGAGTGGAGGCACCACCAC+CGG 0.520780 3.1:+85134505 None:intergenic
GTCTGAGGAGTCAGAGGAGA+AGG 0.523791 3.1:+85134574 None:intergenic
CCAGTCACCGGATTTCCAGC+CGG 0.530047 3.1:+85134481 None:intergenic
TTCTTGACTCCCCACATCGT+TGG 0.531867 3.1:+85134795 None:intergenic
ACCGGCCCTGATGTCCGTCC+TGG 0.532481 3.1:-85134689 MS.gene32612:CDS
GGTCCAAGGGAATCTAGTAC+TGG 0.534715 3.1:-85134095 MS.gene32612:CDS
TTTGGAGGAGGCAGCGCCGG+TGG 0.535377 3.1:-85134521 MS.gene32612:CDS
GAGAATCTTGCCAACGATGT+GGG 0.536610 3.1:-85134805 MS.gene32612:CDS
GGTGACTGGTATTGTACTGT+TGG 0.542639 3.1:-85134668 MS.gene32612:CDS
ACATCAGGGCCGGTGGTGAA+AGG 0.548866 3.1:+85134698 None:intergenic
ATTCCCTTGGACCGTTGCAT+CGG 0.549976 3.1:+85134105 None:intergenic
TTTCCAGCCGGGGCGAGTGG+AGG 0.551998 3.1:+85134493 None:intergenic
CCGGTGACTGGATATGCACC+AGG 0.562370 3.1:-85134469 MS.gene32612:intron
CTGCCTCCTCCAAAACCGTA+TGG 0.574316 3.1:+85134530 None:intergenic
ACCAGGACGGACATCAGGGC+CGG 0.576233 3.1:+85134688 None:intergenic
AAAGGAGCCACCAAAATCAA+CGG 0.591777 3.1:+85134751 None:intergenic
CTACCGATGCAACGGTCCAA+GGG 0.596798 3.1:-85134108 MS.gene32612:CDS
GGTGGTGCCTCCACTCGCCC+CGG 0.597153 3.1:-85134500 MS.gene32612:CDS
CCTGGTGCATATCCAGTCAC+CGG 0.599174 3.1:+85134469 None:intergenic
AGAGAATCTTGCCAACGATG+TGG 0.600788 3.1:-85134806 MS.gene32612:CDS
AGAATCTTGCCAACGATGTG+GGG 0.604150 3.1:-85134804 MS.gene32612:CDS
CAATAATCGTGGCATGTCTG+AGG 0.609735 3.1:+85134559 None:intergenic
TGGCATGTCTGAGGAGTCAG+AGG 0.612841 3.1:+85134568 None:intergenic
AGGACGGACATCAGGGCCGG+TGG 0.617823 3.1:+85134691 None:intergenic
TCAAGAATGATTTCTGGCGG+CGG 0.619694 3.1:-85134779 MS.gene32612:CDS
ATGGAATGCTACCGATGCAA+CGG 0.621720 3.1:-85134116 MS.gene32612:CDS
TATTGTACTGTTGGAAACTG+TGG 0.623871 3.1:-85134659 MS.gene32612:CDS
CGATTATTGAGATCACCATA+CGG 0.625229 3.1:-85134545 MS.gene32612:CDS
AGTCACCAGGACGGACATCA+GGG 0.629819 3.1:+85134684 None:intergenic
GCCGGTGGTGAAAGGAAAAG+GGG 0.632818 3.1:+85134706 None:intergenic
ACAGTACAATACCAGTCACC+AGG 0.642353 3.1:+85134671 None:intergenic
GAGTCAAGAATGATTTCTGG+CGG 0.656995 3.1:-85134782 MS.gene32612:CDS
TACAATACCAGTCACCAGGA+CGG 0.657561 3.1:+85134675 None:intergenic
TATGGTGATCTCAATAATCG+TGG 0.671531 3.1:+85134548 None:intergenic
GAAGGTATCAATATCCTTAG+GGG 0.684912 3.1:+85134592 None:intergenic
AGTCACCGGATTTCCAGCCG+GGG 0.690621 3.1:+85134483 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTATAAGTTCCAAATT+AGG + chr3.1:85134535-85134554 None:intergenic 15.0%
!! CTAACAAAATCTCATAAAAA+TGG - chr3.1:85134631-85134650 MS.gene32612:CDS 20.0%
!! GATACTTCTTATAAATATTG+TGG - chr3.1:85134602-85134621 MS.gene32612:CDS 20.0%
!! TTTCATTTGTCTCATTATAA+GGG + chr3.1:85134731-85134750 None:intergenic 20.0%
!!! CATTTTTATGAGATTTTGTT+AGG + chr3.1:85134633-85134652 None:intergenic 20.0%
! CTTCTTATAAATATTGTGGT+TGG - chr3.1:85134606-85134625 MS.gene32612:CDS 25.0%
! GTTTCATTTGTCTCATTATA+AGG + chr3.1:85134732-85134751 None:intergenic 25.0%
ACATGACACTTATAATGAGA+CGG + chr3.1:85134576-85134595 None:intergenic 30.0%
! ATTCTCTTCTTTGAAAGTTG+AGG + chr3.1:85134117-85134136 None:intergenic 30.0%
!! AGAAGGTATCAATATCCTTA+GGG + chr3.1:85134348-85134367 None:intergenic 30.0%
AGAATTGAAATGTGTAGGTC+TGG - chr3.1:85134769-85134788 MS.gene32612:CDS 35.0%
CGATTATTGAGATCACCATA+CGG - chr3.1:85134391-85134410 MS.gene32612:intron 35.0%
GAAATGATTGACATGAAACC+TGG + chr3.1:85134488-85134507 None:intergenic 35.0%
GAATTGAAATGTGTAGGTCT+GGG - chr3.1:85134770-85134789 MS.gene32612:CDS 35.0%
GACCTACACATTTCAATTCT+CGG + chr3.1:85134769-85134788 None:intergenic 35.0%
TACCGAGAATTGAAATGTGT+AGG - chr3.1:85134764-85134783 MS.gene32612:CDS 35.0%
TATGGTGATCTCAATAATCG+TGG + chr3.1:85134391-85134410 None:intergenic 35.0%
TATTGTACTGTTGGAAACTG+TGG - chr3.1:85134277-85134296 MS.gene32612:intron 35.0%
!! GAAGGTATCAATATCCTTAG+GGG + chr3.1:85134347-85134366 None:intergenic 35.0%
!! GAGAAGGTATCAATATCCTT+AGG + chr3.1:85134349-85134368 None:intergenic 35.0%
AAAGGAGCCACCAAAATCAA+CGG + chr3.1:85134188-85134207 None:intergenic 40.0%
CAAATTAGGACGAAGTAGCA+AGG + chr3.1:85134521-85134540 None:intergenic 40.0%
CAATTCTCGGTAATGTCAGT+AGG + chr3.1:85134756-85134775 None:intergenic 40.0%
GCATAACTTCGCCAATAGAA+TGG - chr3.1:85134801-85134820 MS.gene32612:CDS 40.0%
TCGGTAGCATTCCATTCTAT+TGG + chr3.1:85134815-85134834 None:intergenic 40.0%
! CTCTTCTTTGAAAGTTGAGG+TGG + chr3.1:85134114-85134133 None:intergenic 40.0%
! CTTGCTACTTCGTCCTAATT+TGG - chr3.1:85134519-85134538 MS.gene32612:CDS 40.0%
! GAGTCAAGAATGATTTCTGG+CGG - chr3.1:85134154-85134173 MS.gene32612:intron 40.0%
! TTGAGATCACCATACGGTTT+TGG - chr3.1:85134397-85134416 MS.gene32612:intron 40.0%
ACAGTACAATACCAGTCACC+AGG + chr3.1:85134268-85134287 None:intergenic 45.0%
AGAATCTTGCCAACGATGTG+GGG - chr3.1:85134132-85134151 MS.gene32612:CDS 45.0%
AGAGAATCTTGCCAACGATG+TGG - chr3.1:85134130-85134149 MS.gene32612:CDS 45.0%
ATGGAATGCTACCGATGCAA+CGG - chr3.1:85134820-85134839 MS.gene32612:CDS 45.0%
CAATAATCGTGGCATGTCTG+AGG + chr3.1:85134380-85134399 None:intergenic 45.0%
CTTCCAGTACTAGATTCCCT+TGG + chr3.1:85134847-85134866 None:intergenic 45.0%
GAGAATCTTGCCAACGATGT+GGG - chr3.1:85134131-85134150 MS.gene32612:CDS 45.0%
GGGGAGTCAAGAATGATTTC+TGG - chr3.1:85134151-85134170 MS.gene32612:CDS 45.0%
GGTGACTGGTATTGTACTGT+TGG - chr3.1:85134268-85134287 MS.gene32612:intron 45.0%
TACAATACCAGTCACCAGGA+CGG + chr3.1:85134264-85134283 None:intergenic 45.0%
!! AGATCACCATACGGTTTTGG+AGG - chr3.1:85134400-85134419 MS.gene32612:intron 45.0%
!! GATTTTGGTGGCTCCTTTCT+TGG - chr3.1:85134190-85134209 MS.gene32612:intron 45.0%
!! TCAAGAATGATTTCTGGCGG+CGG - chr3.1:85134157-85134176 MS.gene32612:intron 45.0%
AGAGCCTCTTCCACCAAGAA+AGG + chr3.1:85134206-85134225 None:intergenic 50.0%
ATTCCCTTGGACCGTTGCAT+CGG + chr3.1:85134834-85134853 None:intergenic 50.0%
CTTCAAATGTGGTGCCCCTA+AGG - chr3.1:85134330-85134349 MS.gene32612:intron 50.0%
GGTCCAAGGGAATCTAGTAC+TGG - chr3.1:85134841-85134860 MS.gene32612:CDS 50.0%
TTCTTGACTCCCCACATCGT+TGG + chr3.1:85134144-85134163 None:intergenic 50.0%
! CACATTTGAAGCAGCTGGAG+CGG + chr3.1:85134321-85134340 None:intergenic 50.0%
! TCCCCTTTTCCTTTCACCAC+CGG - chr3.1:85134229-85134248 MS.gene32612:intron 50.0%
! TTTGGTGGCTCCTTTCTTGG+TGG - chr3.1:85134193-85134212 MS.gene32612:intron 50.0%
!!! TCACCATACGGTTTTGGAGG+AGG - chr3.1:85134403-85134422 MS.gene32612:intron 50.0%
AGTCACCAGGACGGACATCA+GGG + chr3.1:85134255-85134274 None:intergenic 55.0%
CCTGGTGCATATCCAGTCAC+CGG + chr3.1:85134470-85134489 None:intergenic 55.0%
CGCTCCAGCTGCTTCAAATG+TGG - chr3.1:85134319-85134338 MS.gene32612:intron 55.0%
CTACCGATGCAACGGTCCAA+GGG - chr3.1:85134828-85134847 MS.gene32612:CDS 55.0%
CTGCCTCCTCCAAAACCGTA+TGG + chr3.1:85134409-85134428 None:intergenic 55.0%
GACCAGGAGATTGGAACTGC+AGG - chr3.1:85134080-85134099 MS.gene32612:CDS 55.0%
GCCGGTGGTGAAAGGAAAAG+GGG + chr3.1:85134233-85134252 None:intergenic 55.0%
GGCCGGTGGTGAAAGGAAAA+GGG + chr3.1:85134234-85134253 None:intergenic 55.0%
GGCTCCTTTCTTGGTGGAAG+AGG - chr3.1:85134199-85134218 MS.gene32612:intron 55.0%
GTCCTGCAGTTCCAATCTCC+TGG + chr3.1:85134085-85134104 None:intergenic 55.0%
GTCTGAGGAGTCAGAGGAGA+AGG + chr3.1:85134365-85134384 None:intergenic 55.0%
TGGCATGTCTGAGGAGTCAG+AGG + chr3.1:85134371-85134390 None:intergenic 55.0%
! GGCACCACATTTGAAGCAGC+TGG + chr3.1:85134326-85134345 None:intergenic 55.0%
!! AGAATGATTTCTGGCGGCGG+CGG - chr3.1:85134160-85134179 MS.gene32612:intron 55.0%
ACATCAGGGCCGGTGGTGAA+AGG + chr3.1:85134241-85134260 None:intergenic 60.0%
AGTCACCGGATTTCCAGCCG+GGG + chr3.1:85134456-85134475 None:intergenic 60.0%
CAGTCACCAGGACGGACATC+AGG + chr3.1:85134256-85134275 None:intergenic 60.0%
CAGTCACCGGATTTCCAGCC+GGG + chr3.1:85134457-85134476 None:intergenic 60.0%
CCAGTCACCGGATTTCCAGC+CGG + chr3.1:85134458-85134477 None:intergenic 60.0%
CCGGTGACTGGATATGCACC+AGG - chr3.1:85134467-85134486 MS.gene32612:intron 60.0%
CTGATGTCCGTCCTGGTGAC+TGG - chr3.1:85134254-85134273 MS.gene32612:intron 60.0%
GCTACCGATGCAACGGTCCA+AGG - chr3.1:85134827-85134846 MS.gene32612:CDS 60.0%
GGGCCGGTGGTGAAAGGAAA+AGG + chr3.1:85134235-85134254 None:intergenic 60.0%
! TTTGAAGCAGCTGGAGCGGC+TGG + chr3.1:85134317-85134336 None:intergenic 60.0%
ACCAGGACGGACATCAGGGC+CGG + chr3.1:85134251-85134270 None:intergenic 65.0%
ACTCGCCCCGGCTGGAAATC+CGG - chr3.1:85134448-85134467 MS.gene32612:intron 65.0%
CCGGCTGGAAATCCGGTGAC+TGG - chr3.1:85134455-85134474 MS.gene32612:intron 65.0%
!!! GGCGGCGCCGTTGATTTTGG+TGG - chr3.1:85134178-85134197 MS.gene32612:intron 65.0%
!!! GGTTTTGGAGGAGGCAGCGC+CGG - chr3.1:85134412-85134431 MS.gene32612:intron 65.0%
ACCGGCCCTGATGTCCGTCC+TGG - chr3.1:85134247-85134266 MS.gene32612:intron 70.0%
AGGACGGACATCAGGGCCGG+TGG + chr3.1:85134248-85134267 None:intergenic 70.0%
GGATTTCCAGCCGGGGCGAG+TGG + chr3.1:85134449-85134468 None:intergenic 70.0%
TTTCCAGCCGGGGCGAGTGG+AGG + chr3.1:85134446-85134465 None:intergenic 70.0%
! TTTGGAGGAGGCAGCGCCGG+TGG - chr3.1:85134415-85134434 MS.gene32612:intron 70.0%
!! GCGAGTGGAGGCACCACCAC+CGG + chr3.1:85134434-85134453 None:intergenic 70.0%
!!! GGCGGCGGCGCCGTTGATTT+TGG - chr3.1:85134175-85134194 MS.gene32612:intron 70.0%
GGTGGTGCCTCCACTCGCCC+CGG - chr3.1:85134436-85134455 MS.gene32612:intron 75.0%
! GTGCCTCCACTCGCCCCGGC+TGG - chr3.1:85134440-85134459 MS.gene32612:intron 80.0%
!!! GGAGGAGGCAGCGCCGGTGG+TGG - chr3.1:85134418-85134437 MS.gene32612:intron 80.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 85134073 85134885 85134073 ID=MS.gene32612
chr3.1 mRNA 85134073 85134885 85134073 ID=MS.gene32612.t1;Parent=MS.gene32612
chr3.1 exon 85134470 85134885 85134470 ID=MS.gene32612.t1.exon1;Parent=MS.gene32612.t1
chr3.1 CDS 85134470 85134885 85134470 ID=cds.MS.gene32612.t1;Parent=MS.gene32612.t1
chr3.1 exon 85134073 85134172 85134073 ID=MS.gene32612.t1.exon2;Parent=MS.gene32612.t1
chr3.1 CDS 85134073 85134172 85134073 ID=cds.MS.gene32612.t1;Parent=MS.gene32612.t1
Gene Sequence

>MS.gene32612

ATGAGCAGACCAGGAGATTGGAACTGCAGGACATGCAACCACCTCAACTTTCAAAGAAGAGAATCTTGCCAACGATGTGGGGAGTCAAGAATGATTTCTGGCGGCGGCGGCGCCGTTGATTTTGGTGGCTCCTTTCTTGGTGGAAGAGGCTCTAGCTCCCCTTTTCCTTTCACCACCGGCCCTGATGTCCGTCCTGGTGACTGGTATTGTACTGTTGGAAACTGTGGAGCTCACAACTTTGCCAGCCGCTCCAGCTGCTTCAAATGTGGTGCCCCTAAGGATATTGATACCTTCTCCTCTGACTCCTCAGACATGCCACGATTATTGAGATCACCATACGGTTTTGGAGGAGGCAGCGCCGGTGGTGGTGCCTCCACTCGCCCCGGCTGGAAATCCGGTGACTGGATATGCACCAGGTTTCATGTCAATCATTTCTTTCATTATACCTTGCTACTTCGTCCTAATTTGGAACTTATAATATATACTAGCATATACTTACTCCGTCTCATTATAAGTGTCATGTTAACGTGATACTTCTTATAAATATTGTGGTTGGACCTAACAAAATCTCATAAAAATGGTTTGTAAGTTTCACTTATAAACATGATCATCTCTCATCAAATATGAAACTTTTAACGTGACACTATTAATGCGACCCTTATAATGAGACAAATGAAACTCCTACTGACATTACCGAGAATTGAAATGTGTAGGTCTGGGTGTAACGAGCATAACTTCGCCAATAGAATGGAATGCTACCGATGCAACGGTCCAAGGGAATCTAGTACTGGAAGATCTTCCTATTTATCGTGA

Protein sequence

>MS.gene32612.t1

MSRPGDWNCRTCNHLNFQRRESCQRCGESRMISGGGGAVDFGGSFLGGRGSSSPFPFTTGPDVRPGDWYCTVGNCGAHNFASRSSCFKCGAPKDIDTFSSDSSDMPRLLRSPYGFGGGSAGGGASTRPGWKSGDWICTRSGCNEHNFANRMECYRCNGPRESSTGRSSYLS