AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62016

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62016.t1 MTR_3g450850 69.373 271 82 1 31 300 16 286 2.58e-131 392
MS.gene62016.t1 MTR_4g035820 37.736 318 174 6 60 370 74 374 4.31e-52 192
MS.gene62016.t1 MTR_4g035820 33.659 205 108 3 449 653 326 502 7.46e-22 100
MS.gene62016.t1 MTR_3g012160 83.696 92 15 0 210 301 2 93 1.34e-46 160
MS.gene62016.t1 MTR_4g071400 96.203 79 3 0 574 652 43 121 1.29e-43 153
MS.gene62016.t1 MTR_1g070355 32.540 252 150 7 61 306 72 309 7.79e-29 122
MS.gene62016.t1 MTR_6g051425 27.208 283 194 5 62 341 18 291 5.38e-27 113
MS.gene62016.t1 MTR_5g076070 27.917 240 157 7 64 300 56 282 2.53e-23 100
MS.gene62016.t1 MTR_3g095940 74.074 54 14 0 514 567 90 143 3.60e-20 88.2
MS.gene62016.t1 MTR_5g083630 57.143 70 24 1 204 267 7 76 7.24e-18 80.1
MS.gene62016.t1 MTR_6g014355 37.013 154 81 5 142 287 14 159 9.77e-17 80.9
MS.gene62016.t1 MTR_6g022080 30.909 165 97 6 72 232 96 247 4.90e-14 73.2
MS.gene62016.t1 MTR_0558s0020 24.715 263 175 7 66 312 41 296 6.18e-14 73.6
MS.gene62016.t1 MTR_8g446020 26.178 191 131 4 126 312 72 256 1.72e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62016.t1 AT1G10240 48.000 550 272 7 30 572 11 553 0.0 533
MS.gene62016.t1 AT1G10240 48.000 550 272 7 30 572 11 553 0.0 533
MS.gene62016.t1 AT5G28530 42.545 550 291 4 31 570 27 561 8.97e-160 477
MS.gene62016.t1 AT5G28530 42.545 550 291 4 31 570 27 561 8.97e-160 477
MS.gene62016.t1 AT5G28530 42.364 550 296 4 31 570 27 565 4.16e-159 475
MS.gene62016.t1 AT5G28530 38.148 540 266 7 31 570 27 498 1.07e-124 384
MS.gene62016.t1 AT5G28530 37.963 540 271 7 31 570 27 502 4.99e-124 382
MS.gene62016.t1 AT4G38180 30.255 509 341 6 61 566 73 570 2.95e-68 239
MS.gene62016.t1 AT4G15090 30.408 490 319 10 61 544 51 524 1.04e-67 238
MS.gene62016.t1 AT4G15090 30.408 490 319 10 61 544 51 524 1.04e-67 238
MS.gene62016.t1 AT4G15090 30.408 490 319 10 61 544 51 524 1.04e-67 238
MS.gene62016.t1 AT4G15090 30.408 490 319 10 61 544 51 524 1.04e-67 238
MS.gene62016.t1 AT4G15090 30.408 490 319 10 61 544 51 524 1.04e-67 238
MS.gene62016.t1 AT3G22170 27.734 512 321 16 49 544 55 533 5.01e-54 199
MS.gene62016.t1 AT3G22170 27.734 512 321 16 49 544 55 533 5.01e-54 199
MS.gene62016.t1 AT1G76320 27.869 488 313 12 67 544 3 461 8.83e-52 191
MS.gene62016.t1 AT1G76320 27.869 488 313 12 67 544 3 461 9.45e-52 192
MS.gene62016.t1 AT1G76320 27.869 488 313 12 67 544 3 461 1.01e-51 192
MS.gene62016.t1 AT1G76320 27.869 488 313 12 67 544 3 461 1.01e-51 192
MS.gene62016.t1 AT3G06250 25.800 500 306 12 61 555 190 629 5.68e-46 175
MS.gene62016.t1 AT3G06250 25.800 500 306 12 61 555 190 629 5.68e-46 175
MS.gene62016.t1 AT3G06250 25.800 500 306 12 61 555 190 629 5.68e-46 175
MS.gene62016.t1 AT5G18960 25.725 517 316 12 47 555 197 653 1.81e-45 174
MS.gene62016.t1 AT5G18960 25.725 517 316 12 47 555 197 653 1.81e-45 174
MS.gene62016.t1 AT2G27110 25.514 486 319 12 61 544 49 493 3.54e-40 158
MS.gene62016.t1 AT2G27110 25.514 486 319 12 61 544 49 493 3.54e-40 158
MS.gene62016.t1 AT2G32250 27.586 290 208 2 256 544 180 468 2.71e-39 155
MS.gene62016.t1 AT2G32250 27.586 290 208 2 256 544 139 427 3.80e-39 155
MS.gene62016.t1 AT2G32250 27.586 290 208 2 256 544 180 468 3.98e-39 155
MS.gene62016.t1 AT2G32250 27.586 290 208 2 256 544 180 468 3.98e-39 155
MS.gene62016.t1 AT2G32250 27.586 290 208 2 256 544 180 468 3.98e-39 155
MS.gene62016.t1 AT2G32250 27.586 290 208 2 256 544 139 427 4.01e-39 155
MS.gene62016.t1 AT2G32250 27.586 290 208 2 256 544 180 468 4.21e-39 155
MS.gene62016.t1 AT2G32250 27.586 290 208 2 256 544 180 468 4.21e-39 155
MS.gene62016.t1 AT4G19990 26.441 503 296 14 63 544 23 472 9.61e-38 150
MS.gene62016.t1 AT4G19990 26.441 503 296 14 63 544 23 472 1.02e-37 150
MS.gene62016.t1 AT4G19990 26.441 503 296 14 63 544 23 472 1.02e-37 150
MS.gene62016.t1 AT4G19990 26.441 503 296 14 63 544 37 486 1.41e-37 150
MS.gene62016.t1 AT4G19990 26.441 503 296 14 63 544 23 472 1.58e-37 150
MS.gene62016.t1 AT4G19990 26.441 503 296 14 63 544 23 472 1.58e-37 150
MS.gene62016.t1 AT1G52520 25.761 493 327 14 61 545 85 546 1.56e-36 146
MS.gene62016.t1 AT2G27110 28.013 307 213 5 240 544 48 348 4.65e-36 145
MS.gene62016.t1 AT4G19990 25.835 449 275 11 112 544 9 415 2.90e-33 136
MS.gene62016.t1 AT1G80010 23.862 549 323 17 61 563 95 594 1.24e-32 135
MS.gene62016.t1 AT4G38170 27.457 346 240 6 244 583 16 356 6.90e-31 128

Find 119 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTACCCATCGTAAATTT+TGG 0.158198 7.2:-80084183 MS.gene62016:CDS
CCAATTAAAACTTACTTATT+AGG 0.177601 7.2:+80083152 None:intergenic
ATGAGATGCCTCTTGGAATA+TGG 0.196486 7.2:-80084684 MS.gene62016:CDS
TTCTCACAACCACGAGTTAT+TGG 0.199264 7.2:-80085106 MS.gene62016:CDS
ATCACATGTTCTTGAGTTTC+AGG 0.210618 7.2:+80083395 None:intergenic
CTCATGTTGAAGCAACTTAA+AGG 0.248230 7.2:+80083829 None:intergenic
TGTTTATTTGCAAAACAAAA+TGG 0.260407 7.2:-80085372 MS.gene62016:CDS
AAAATCTGTCAAACTTGTTT+TGG 0.261322 7.2:+80084066 None:intergenic
ATTCGTGCATATGAAGCTTT+TGG 0.265394 7.2:-80084748 MS.gene62016:CDS
CAATATGAATTCACAATTGA+TGG 0.266214 7.2:-80084814 MS.gene62016:CDS
TTCCTTCGCATGGGCTATAA+AGG 0.299718 7.2:-80084593 MS.gene62016:intron
AATATCATCATAAAGATAAA+AGG 0.310779 7.2:-80083422 MS.gene62016:CDS
TCAAGAACATGTGATTGATT+TGG 0.314506 7.2:-80083387 MS.gene62016:CDS
CATCAATTGTGAATTCATAT+TGG 0.326055 7.2:+80084815 None:intergenic
GCAGCTTCAACCCCTCCTTT+TGG 0.339248 7.2:+80083281 None:intergenic
ATTCAATCATATAGTGGTAT+TGG 0.341059 7.2:-80084904 MS.gene62016:CDS
ACTCTCGCTATAAGACTTAC+AGG 0.343612 7.2:+80084994 None:intergenic
CAAATTGGAGCATATTGTTT+GGG 0.347369 7.2:-80084785 MS.gene62016:CDS
GAGCATATTGTTTGGGCATT+TGG 0.348974 7.2:-80084778 MS.gene62016:CDS
ACAAATTGGAGCATATTGTT+TGG 0.352856 7.2:-80084786 MS.gene62016:CDS
AATTGATGGAAGTAACAAAT+TGG 0.358783 7.2:-80084800 MS.gene62016:CDS
ATGACAACGACGAGGCTTCT+TGG 0.366012 7.2:+80083259 None:intergenic
TCAGTCTCTAATTCGATGTT+TGG 0.372448 7.2:-80083501 MS.gene62016:intron
AACGTCACAGTCAGCATTGT+TGG 0.377007 7.2:+80083352 None:intergenic
ATGGTCTCACAGTTTGGTTT+GGG 0.387072 7.2:-80084229 MS.gene62016:CDS
GTAGTTACTAAGAGAAGAAT+TGG 0.389295 7.2:-80085165 MS.gene62016:CDS
AATGGTCTCACAGTTTGGTT+TGG 0.398201 7.2:-80084230 MS.gene62016:CDS
CCTCAGGTCACTAACATTGA+TGG 0.404443 7.2:-80085561 MS.gene62016:CDS
TAGTGATTGGCCCTTGCCTC+AGG 0.405183 7.2:-80085577 MS.gene62016:CDS
GCAATGGCTTCTTTAATTGA+AGG 0.406562 7.2:+80084421 None:intergenic
TCAAATATTTGCCCTTCAAA+AGG 0.411166 7.2:+80085531 None:intergenic
ATCCATCGGGTGTTTATAAG+AGG 0.417116 7.2:-80085293 MS.gene62016:CDS
TTTGCTGGAATGACAACAAC+AGG 0.418779 7.2:-80084133 MS.gene62016:CDS
AAAGTAATGGTCTCACAGTT+TGG 0.427267 7.2:-80084235 MS.gene62016:CDS
TGAGATGCCTCTTGGAATAT+GGG 0.433904 7.2:-80084683 MS.gene62016:CDS
TTGGGCCTTAGCTTATTTGA+AGG 0.435274 7.2:-80084164 MS.gene62016:CDS
TATACGACCACCATCAACTT+TGG 0.437731 7.2:+80083739 None:intergenic
CTTTAAACATGAGTTTCATA+AGG 0.439926 7.2:-80084299 MS.gene62016:CDS
GACGAGGCATGTTCTTCAAT+AGG 0.443666 7.2:+80083869 None:intergenic
TGATTAGTTCCACCACAATC+TGG 0.446933 7.2:+80083233 None:intergenic
AACCGTTATGAGATGCCTCT+TGG 0.448749 7.2:-80084691 MS.gene62016:CDS
AAAGACAAGTGAACGAGAAC+AGG 0.453486 7.2:-80083465 MS.gene62016:CDS
GGAAAATGTGTTGTAATATG+AGG 0.458305 7.2:+80083890 None:intergenic
GTTTGTCATCGTGGTGGTAT+TGG 0.458689 7.2:-80085264 MS.gene62016:CDS
ATGCGATCTTGATCAACAAT+TGG 0.465554 7.2:+80085042 None:intergenic
CTCACAGTTTGGTTTGGGTG+TGG 0.468983 7.2:-80084224 MS.gene62016:CDS
ATAACGGTTTATTCTATATG+TGG 0.472493 7.2:+80084705 None:intergenic
TATAAGACTTACAGGGCAAC+CGG 0.483233 7.2:+80085002 None:intergenic
CTTGAGTTAGAGAAAAGAAC+AGG 0.494786 7.2:-80084970 MS.gene62016:CDS
AAAACAAAAGGTCGGCCAAA+AGG 0.495104 7.2:-80083311 MS.gene62016:CDS
TTGATAGGATCATGAAGGGT+TGG 0.498564 7.2:-80083065 MS.gene62016:intron
TGAAGCAACTTAAAGGCATA+AGG 0.499495 7.2:+80083836 None:intergenic
AGAAGATTTCTTCCTTCGCA+TGG 0.503824 7.2:-80084603 MS.gene62016:CDS
CGTTGTCATGTTCCAGATTG+TGG 0.504732 7.2:-80083245 MS.gene62016:CDS
AATTGTGAATTCATATTGGA+AGG 0.507660 7.2:+80084819 None:intergenic
CCAAAAGGATCAAGACCAAA+AGG 0.514192 7.2:-80083296 MS.gene62016:CDS
GTCAAAATTAGAAATCAAGC+TGG 0.517568 7.2:-80083947 MS.gene62016:CDS
TTTAATTGAAGGATCTTGAT+CGG 0.518039 7.2:+80084432 None:intergenic
ATTGGAATGTTACGATAAGC+AGG 0.522557 7.2:+80085060 None:intergenic
ATGTTATTGACTTGAGGCAA+GGG 0.525418 7.2:+80085459 None:intergenic
TAAGCAATGATGCTAGTGAT+TGG 0.525487 7.2:-80085590 MS.gene62016:CDS
ACCACGCAAAACTGTTGAAG+TGG 0.526679 7.2:-80085238 MS.gene62016:CDS
TTAGAGAAAAGAACAGGCAC+AGG 0.528687 7.2:-80084964 MS.gene62016:CDS
TGGGTTGGTGTTGATAATCA+TGG 0.529258 7.2:-80084664 MS.gene62016:CDS
ATACTATTATTGTCTAAAGC+CGG 0.531055 7.2:-80085021 MS.gene62016:CDS
TTCCAAGAGGCATCTCATAA+CGG 0.531646 7.2:+80084689 None:intergenic
CTCAGGTCACTAACATTGAT+GGG 0.537467 7.2:-80085560 MS.gene62016:CDS
CAACATCATACCAAAGTTGA+TGG 0.538288 7.2:-80083749 MS.gene62016:CDS
TGAACGAGAATGGAAAGTAA+TGG 0.544871 7.2:-80084248 MS.gene62016:CDS
TTGCACAACATAAGAGCCAT+TGG 0.551354 7.2:+80083769 None:intergenic
TAAGAGAAGAATTGGCATTG+AGG 0.553953 7.2:-80085157 MS.gene62016:CDS
GAAGATTTCTTCCTTCGCAT+GGG 0.556681 7.2:-80084602 MS.gene62016:CDS
GTGCTTTGATAGGATCATGA+AGG 0.556862 7.2:-80083070 MS.gene62016:intron
ATGTTAGTGACCTGAGGCAA+GGG 0.557252 7.2:+80085567 None:intergenic
CAAGTCAATAACATTGATGA+GGG 0.558520 7.2:-80085450 MS.gene62016:CDS
AAAGTCCTTCAAATAAGCTA+AGG 0.560983 7.2:+80084159 None:intergenic
ACATGTGATTGATTTGGAAG+AGG 0.565753 7.2:-80083381 MS.gene62016:CDS
GTTTGGCAATTCCATTGCAA+TGG 0.567761 7.2:+80084405 None:intergenic
ATGTGATTGTAGTGCAAAGA+TGG 0.570079 7.2:-80085187 MS.gene62016:CDS
GTGTCAGAAGAGAATCCATC+GGG 0.572540 7.2:-80085306 MS.gene62016:CDS
TCATCAATGTTATTGACTTG+AGG 0.573525 7.2:+80085453 None:intergenic
GGTCACTAACATTGATGGGT+GGG 0.575865 7.2:-80085556 MS.gene62016:CDS
AATTAAAGAAGCCATTGCAA+TGG 0.576797 7.2:-80084416 MS.gene62016:CDS
TAGAAATCAAGCTGGAGAAG+AGG 0.577533 7.2:-80083939 MS.gene62016:CDS
CGAGTTATTGGATGACAAAG+AGG 0.580002 7.2:-80085094 MS.gene62016:CDS
AATGTTATTGACTTGAGGCA+AGG 0.580693 7.2:+80085458 None:intergenic
AATGTTAGTGACCTGAGGCA+AGG 0.580804 7.2:+80085566 None:intergenic
TTGTCGCAAAGCTGTCAAGT+TGG 0.589381 7.2:-80084354 MS.gene62016:CDS
TCATCATATCTCGAACCTAG+TGG 0.597376 7.2:+80084322 None:intergenic
AGGGAATTTGTTTGTCATCG+TGG 0.598966 7.2:-80085273 MS.gene62016:CDS
TCCCTCTTATAAACACCCGA+TGG 0.599604 7.2:+80085291 None:intergenic
GCAGCAATTGAAGCTGCCAA+TGG 0.600590 7.2:-80083785 MS.gene62016:CDS
TACCTTTATAGCCCATGCGA+AGG 0.601051 7.2:+80084591 None:intergenic
TCAAGTCAATAACATTGATG+AGG 0.603097 7.2:-80085451 MS.gene62016:CDS
CTGTTGAAGTGGAAAATCAA+AGG 0.606328 7.2:-80085227 MS.gene62016:CDS
CTTTGTCATCCAATAACTCG+TGG 0.606716 7.2:+80085097 None:intergenic
AAGGATCAAGACCAAAAGGA+GGG 0.611165 7.2:-80083292 MS.gene62016:CDS
CTAAGGCCCAAAATTTACGA+TGG 0.615020 7.2:+80084176 None:intergenic
TAAGGCCCAAAATTTACGAT+GGG 0.615697 7.2:+80084177 None:intergenic
ATGCCTCTTGGAATATGGGT+TGG 0.616141 7.2:-80084679 MS.gene62016:CDS
GAATTTGTTTGTCATCGTGG+TGG 0.618777 7.2:-80085270 MS.gene62016:CDS
AGGTCACTAACATTGATGGG+TGG 0.619738 7.2:-80085557 MS.gene62016:CDS
TCCATCGGGTGTTTATAAGA+GGG 0.625908 7.2:-80085292 MS.gene62016:CDS
ACGAGAACAGGTTGCCACAA+GGG 0.630576 7.2:-80083453 MS.gene62016:CDS
TGTGTCAGAAGAGAATCCAT+CGG 0.632507 7.2:-80085307 MS.gene62016:CDS
AACGAGAACAGGTTGCCACA+AGG 0.633734 7.2:-80083454 MS.gene62016:CDS
TACTTATTAGGAGATTGAGA+AGG 0.635742 7.2:+80083164 None:intergenic
TGTCATGTTCCAGATTGTGG+TGG 0.637809 7.2:-80083242 MS.gene62016:CDS
CTCTCGCTATAAGACTTACA+GGG 0.647869 7.2:+80084995 None:intergenic
AAAGGATCAAGACCAAAAGG+AGG 0.655085 7.2:-80083293 MS.gene62016:CDS
CCATCAATGTTAGTGACCTG+AGG 0.674244 7.2:+80085561 None:intergenic
ACACCAACCCATATTCCAAG+AGG 0.676376 7.2:+80084676 None:intergenic
TGCTTTGATAGGATCATGAA+GGG 0.676443 7.2:-80083069 MS.gene62016:intron
AAGTTTGAAAGATCGAGATG+AGG 0.682349 7.2:-80084842 MS.gene62016:CDS
CATCATACCAAAGTTGATGG+TGG 0.683172 7.2:-80083746 MS.gene62016:CDS
AGGATCAAGACCAAAAGGAG+GGG 0.696841 7.2:-80083291 MS.gene62016:CDS
AAGTCAATAACATTGATGAG+GGG 0.714581 7.2:-80085449 MS.gene62016:CDS
ATAAGGTGTGAGTATAGACG+AGG 0.720548 7.2:+80083853 None:intergenic
TCTGGAACATGACAACGACG+AGG 0.797744 7.2:+80083251 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTAACTGTATTTTTA+TGG - chr7.2:80085570-80085589 MS.gene62016:CDS 10.0%
!! AATATCATCATAAAGATAAA+AGG - chr7.2:80085240-80085259 MS.gene62016:CDS 15.0%
!!! AATTTTATTCAATCATATAG+TGG - chr7.2:80083752-80083771 MS.gene62016:CDS 15.0%
!! CCAATTAAAACTTACTTATT+AGG + chr7.2:80085513-80085532 None:intergenic 20.0%
!! TGTTTATTTGCAAAACAAAA+TGG - chr7.2:80083290-80083309 MS.gene62016:CDS 20.0%
!!! AACAAAATGGATTTTCTATT+AGG - chr7.2:80083303-80083322 MS.gene62016:CDS 20.0%
!!! CATGGAAATTCAATTTTTTT+TGG - chr7.2:80084016-80084035 MS.gene62016:intron 20.0%
!!! CCTAATAAGTAAGTTTTAAT+TGG - chr7.2:80085510-80085529 MS.gene62016:CDS 20.0%
!!! CTAATATCTTTTTCTAAGAA+TGG + chr7.2:80083731-80083750 None:intergenic 20.0%
!!! TGATTGTTATATTTTTGTGT+AGG - chr7.2:80084681-80084700 MS.gene62016:CDS 20.0%
!!! TTATTACATGACTAGATTTT+GGG + chr7.2:80085113-80085132 None:intergenic 20.0%
!!! TTTATTACATGACTAGATTT+TGG + chr7.2:80085114-80085133 None:intergenic 20.0%
! AATTGATGGAAGTAACAAAT+TGG - chr7.2:80083862-80083881 MS.gene62016:CDS 25.0%
! AATTGTGAATTCATATTGGA+AGG + chr7.2:80083846-80083865 None:intergenic 25.0%
! ATAACGGTTTATTCTATATG+TGG + chr7.2:80083960-80083979 None:intergenic 25.0%
! ATTCAATCATATAGTGGTAT+TGG - chr7.2:80083758-80083777 MS.gene62016:CDS 25.0%
! CAATATGAATTCACAATTGA+TGG - chr7.2:80083848-80083867 MS.gene62016:CDS 25.0%
! CATCAATTGTGAATTCATAT+TGG + chr7.2:80083850-80083869 None:intergenic 25.0%
! CTTTAAACATGAGTTTCATA+AGG - chr7.2:80084363-80084382 MS.gene62016:CDS 25.0%
! TCCAAACAAAAAGAGAAAAA+TGG - chr7.2:80085467-80085486 MS.gene62016:CDS 25.0%
! TTGTTTCAAAAACAAAAGGT+CGG - chr7.2:80085343-80085362 MS.gene62016:CDS 25.0%
! TTTAATTGAAGGATCTTGAT+CGG + chr7.2:80084233-80084252 None:intergenic 25.0%
!! ATACTATTATTGTCTAAAGC+CGG - chr7.2:80083641-80083660 MS.gene62016:intron 25.0%
!! TCAAATATTTTCCCTTCAAA+AGG + chr7.2:80083242-80083261 None:intergenic 25.0%
!! TGTAAAGAGTTTCAATTTTC+AGG - chr7.2:80084985-80085004 MS.gene62016:CDS 25.0%
!!! AAAATCTGTCAAACTTGTTT+TGG + chr7.2:80084599-80084618 None:intergenic 25.0%
!!! TATTTTTATGGTGCTTTGAT+AGG - chr7.2:80085582-80085601 MS.gene62016:CDS 25.0%
!!! TCCATTTTTCTCTTTTTGTT+TGG + chr7.2:80085471-80085490 None:intergenic 25.0%
!!! TGTTATATTTTTGTGTAGGT+TGG - chr7.2:80084685-80084704 MS.gene62016:CDS 25.0%
AAAGTCCTTCAAATAAGCTA+AGG + chr7.2:80084506-80084525 None:intergenic 30.0%
AAGTCAATAACATTGATGAG+GGG - chr7.2:80083213-80083232 MS.gene62016:CDS 30.0%
AATTAAAGAAGCCATTGCAA+TGG - chr7.2:80084246-80084265 MS.gene62016:CDS 30.0%
ACAAATTGGAGCATATTGTT+TGG - chr7.2:80083876-80083895 MS.gene62016:CDS 30.0%
CAAATTGGAGCATATTGTTT+GGG - chr7.2:80083877-80083896 MS.gene62016:CDS 30.0%
CAAGTCAATAACATTGATGA+GGG - chr7.2:80083212-80083231 MS.gene62016:CDS 30.0%
CATGTAATAAACTGCAATGA+TGG - chr7.2:80085123-80085142 MS.gene62016:CDS 30.0%
GGAAAATGTGTTGTAATATG+AGG + chr7.2:80084775-80084794 None:intergenic 30.0%
GTAGTTACTAAGAGAAGAAT+TGG - chr7.2:80083497-80083516 MS.gene62016:CDS 30.0%
GTCAAAATTAGAAATCAAGC+TGG - chr7.2:80084715-80084734 MS.gene62016:CDS 30.0%
TACTTATTAGGAGATTGAGA+AGG + chr7.2:80085501-80085520 None:intergenic 30.0%
TCAAATATTTGCCCTTCAAA+AGG + chr7.2:80083134-80083153 None:intergenic 30.0%
TCAAGAACATGTGATTGATT+TGG - chr7.2:80085275-80085294 MS.gene62016:CDS 30.0%
TCAAGTCAATAACATTGATG+AGG - chr7.2:80083211-80083230 MS.gene62016:CDS 30.0%
! AATTGGCATTGAGGAAAAAT+GGG - chr7.2:80083514-80083533 MS.gene62016:intron 30.0%
! ATTTTCCTTTCACAAAAGCT+AGG + chr7.2:80084192-80084211 None:intergenic 30.0%
! CAAAACATGCATTTTGCATA+TGG - chr7.2:80084281-80084300 MS.gene62016:CDS 30.0%
! CAAAATATCTTCCTTTTCGA+TGG - chr7.2:80085067-80085086 MS.gene62016:CDS 30.0%
! CAAAATGCATGTTTTGTGTT+TGG + chr7.2:80084277-80084296 None:intergenic 30.0%
! CTTTACCCATCGTAAATTTT+GGG - chr7.2:80084480-80084499 MS.gene62016:CDS 30.0%
! TCTTTACCCATCGTAAATTT+TGG - chr7.2:80084479-80084498 MS.gene62016:CDS 30.0%
! TGATCGGTAAGAATTGTTTT+TGG + chr7.2:80084217-80084236 None:intergenic 30.0%
!! CCTGTTGTTTCAAAAACAAA+AGG - chr7.2:80085339-80085358 MS.gene62016:CDS 30.0%
!! CGAAAAGGAAGATATTTTGA+TGG + chr7.2:80085066-80085085 None:intergenic 30.0%
!! TCATCAATGTTATTGACTTG+AGG + chr7.2:80083212-80083231 None:intergenic 30.0%
!!! CCTTTTGTTTTTGAAACAAC+AGG + chr7.2:80085342-80085361 None:intergenic 30.0%
!!! TTGAAGGACTTTTTTTTTGC+TGG - chr7.2:80084514-80084533 MS.gene62016:intron 30.0%
AAAGTAATGGTCTCACAGTT+TGG - chr7.2:80084427-80084446 MS.gene62016:CDS 35.0%
AAGTTTGAAAGATCGAGATG+AGG - chr7.2:80083820-80083839 MS.gene62016:CDS 35.0%
ATCACATGTTCTTGAGTTTC+AGG + chr7.2:80085270-80085289 None:intergenic 35.0%
ATGCGATCTTGATCAACAAT+TGG + chr7.2:80083623-80083642 None:intergenic 35.0%
ATGTGATTGTAGTGCAAAGA+TGG - chr7.2:80083475-80083494 MS.gene62016:CDS 35.0%
ATTGGAATGTTACGATAAGC+AGG + chr7.2:80083605-80083624 None:intergenic 35.0%
CAACATCATACCAAAGTTGA+TGG - chr7.2:80084913-80084932 MS.gene62016:CDS 35.0%
CTCATGTTGAAGCAACTTAA+AGG + chr7.2:80084836-80084855 None:intergenic 35.0%
CTGTTGAAGTGGAAAATCAA+AGG - chr7.2:80083435-80083454 MS.gene62016:CDS 35.0%
CTTGAGTTAGAGAAAAGAAC+AGG - chr7.2:80083692-80083711 MS.gene62016:intron 35.0%
GCAATGGCTTCTTTAATTGA+AGG + chr7.2:80084244-80084263 None:intergenic 35.0%
TAAGAGAAGAATTGGCATTG+AGG - chr7.2:80083505-80083524 MS.gene62016:intron 35.0%
TAAGCAATGATGCTAGTGAT+TGG - chr7.2:80083072-80083091 MS.gene62016:intron 35.0%
TAAGGCCCAAAATTTACGAT+GGG + chr7.2:80084488-80084507 None:intergenic 35.0%
TAGAGACTGAAATTTAGCAG+AGG + chr7.2:80085151-80085170 None:intergenic 35.0%
TCAGTCTCTAATTCGATGTT+TGG - chr7.2:80085161-80085180 MS.gene62016:CDS 35.0%
TGAACGAGAATGGAAAGTAA+TGG - chr7.2:80084414-80084433 MS.gene62016:CDS 35.0%
TGCTTTGATAGGATCATGAA+GGG - chr7.2:80085593-80085612 MS.gene62016:CDS 35.0%
! ATTCGTGCATATGAAGCTTT+TGG - chr7.2:80083914-80083933 MS.gene62016:CDS 35.0%
! CAGATGATTTTGAACGAGAA+TGG - chr7.2:80084404-80084423 MS.gene62016:CDS 35.0%
! GAATTGGCATTGAGGAAAAA+TGG - chr7.2:80083513-80083532 MS.gene62016:intron 35.0%
! GATATTTTCTAACTCCCTTG+TGG + chr7.2:80085226-80085245 None:intergenic 35.0%
! TGAAGCAACTTAAAGGCATA+AGG + chr7.2:80084829-80084848 None:intergenic 35.0%
!! AATGTTATTGACTTGAGGCA+AGG + chr7.2:80083207-80083226 None:intergenic 35.0%
!! ACATGTGATTGATTTGGAAG+AGG - chr7.2:80085281-80085300 MS.gene62016:CDS 35.0%
!! AGTTTCCTAGCTTTTGTGAA+AGG - chr7.2:80084184-80084203 MS.gene62016:CDS 35.0%
!! ATGTTATTGACTTGAGGCAA+GGG + chr7.2:80083206-80083225 None:intergenic 35.0%
!!! AGTTGGTTTTCTTTTCCACT+AGG - chr7.2:80084325-80084344 MS.gene62016:CDS 35.0%
AAAACAAAAGGTCGGCCAAA+AGG - chr7.2:80085351-80085370 MS.gene62016:CDS 40.0%
AAAGACAAGTGAACGAGAAC+AGG - chr7.2:80085197-80085216 MS.gene62016:CDS 40.0%
AAAGGATCAAGACCAAAAGG+AGG - chr7.2:80085369-80085388 MS.gene62016:CDS 40.0%
AAGGATCAAGACCAAAAGGA+GGG - chr7.2:80085370-80085389 MS.gene62016:CDS 40.0%
AATGGTCTCACAGTTTGGTT+TGG - chr7.2:80084432-80084451 MS.gene62016:CDS 40.0%
ACTCTCGCTATAAGACTTAC+AGG + chr7.2:80083671-80083690 None:intergenic 40.0%
AGAAGATTTCTTCCTTCGCA+TGG - chr7.2:80084059-80084078 MS.gene62016:CDS 40.0%
ATAAGGTGTGAGTATAGACG+AGG + chr7.2:80084812-80084831 None:intergenic 40.0%
ATCCATCGGGTGTTTATAAG+AGG - chr7.2:80083369-80083388 MS.gene62016:CDS 40.0%
ATGAGATGCCTCTTGGAATA+TGG - chr7.2:80083978-80083997 MS.gene62016:intron 40.0%
CCAAAAGGATCAAGACCAAA+AGG - chr7.2:80085366-80085385 MS.gene62016:CDS 40.0%
CGAGTTATTGGATGACAAAG+AGG - chr7.2:80083568-80083587 MS.gene62016:intron 40.0%
CTAAGGCCCAAAATTTACGA+TGG + chr7.2:80084489-80084508 None:intergenic 40.0%
CTCAGGTCACTAACATTGAT+GGG - chr7.2:80083102-80083121 MS.gene62016:intron 40.0%
CTCTCGCTATAAGACTTACA+GGG + chr7.2:80083670-80083689 None:intergenic 40.0%
CTTTGTCATCCAATAACTCG+TGG + chr7.2:80083568-80083587 None:intergenic 40.0%
GAAGATTTCTTCCTTCGCAT+GGG - chr7.2:80084060-80084079 MS.gene62016:CDS 40.0%
GAGCATATTGTTTGGGCATT+TGG - chr7.2:80083884-80083903 MS.gene62016:CDS 40.0%
GTGCTTTGATAGGATCATGA+AGG - chr7.2:80085592-80085611 MS.gene62016:CDS 40.0%
GTTTGGCAATTCCATTGCAA+TGG + chr7.2:80084260-80084279 None:intergenic 40.0%
TAGAAATCAAGCTGGAGAAG+AGG - chr7.2:80084723-80084742 MS.gene62016:CDS 40.0%
TATAAGACTTACAGGGCAAC+CGG + chr7.2:80083663-80083682 None:intergenic 40.0%
TATACGACCACCATCAACTT+TGG + chr7.2:80084926-80084945 None:intergenic 40.0%
TCATCATATCTCGAACCTAG+TGG + chr7.2:80084343-80084362 None:intergenic 40.0%
TCCATCGGGTGTTTATAAGA+GGG - chr7.2:80083370-80083389 MS.gene62016:CDS 40.0%
TGAGATGCCTCTTGGAATAT+GGG - chr7.2:80083979-80083998 MS.gene62016:intron 40.0%
TGTGTCAGAAGAGAATCCAT+CGG - chr7.2:80083355-80083374 MS.gene62016:CDS 40.0%
TTAGAGAAAAGAACAGGCAC+AGG - chr7.2:80083698-80083717 MS.gene62016:intron 40.0%
TTCTCACAACCACGAGTTAT+TGG - chr7.2:80083556-80083575 MS.gene62016:intron 40.0%
TTGATAGGATCATGAAGGGT+TGG - chr7.2:80085597-80085616 MS.gene62016:CDS 40.0%
TTGCACAACATAAGAGCCAT+TGG + chr7.2:80084896-80084915 None:intergenic 40.0%
TTGGGCCTTAGCTTATTTGA+AGG - chr7.2:80084498-80084517 MS.gene62016:intron 40.0%
TTTGCTGGAATGACAACAAC+AGG - chr7.2:80084529-80084548 MS.gene62016:intron 40.0%
! AGGGAATTTGTTTGTCATCG+TGG - chr7.2:80083389-80083408 MS.gene62016:CDS 40.0%
! ATGAGGGGATTCCTTTTGAA+GGG - chr7.2:80083228-80083247 MS.gene62016:CDS 40.0%
! ATGGTCTCACAGTTTGGTTT+GGG - chr7.2:80084433-80084452 MS.gene62016:CDS 40.0%
! GAATTTGTTTGTCATCGTGG+TGG - chr7.2:80083392-80083411 MS.gene62016:CDS 40.0%
! TGATTAGTTCCACCACAATC+TGG + chr7.2:80085432-80085451 None:intergenic 40.0%
!! CATCATACCAAAGTTGATGG+TGG - chr7.2:80084916-80084935 MS.gene62016:CDS 40.0%
!! TGGGTTGGTGTTGATAATCA+TGG - chr7.2:80083998-80084017 MS.gene62016:intron 40.0%
!! TTCCAAGAGGCATCTCATAA+CGG + chr7.2:80083976-80083995 None:intergenic 40.0%
!!! CCTTTTGGTCTTGATCCTTT+TGG + chr7.2:80085369-80085388 None:intergenic 40.0%
AACCGTTATGAGATGCCTCT+TGG - chr7.2:80083971-80083990 MS.gene62016:intron 45.0%
AACGTCACAGTCAGCATTGT+TGG + chr7.2:80085313-80085332 None:intergenic 45.0%
AATGTTAGTGACCTGAGGCA+AGG + chr7.2:80083099-80083118 None:intergenic 45.0%
ACACCAACCCATATTCCAAG+AGG + chr7.2:80083989-80084008 None:intergenic 45.0%
ACCACGCAAAACTGTTGAAG+TGG - chr7.2:80083424-80083443 MS.gene62016:CDS 45.0%
AGGATCAAGACCAAAAGGAG+GGG - chr7.2:80085371-80085390 MS.gene62016:CDS 45.0%
AGGTCACTAACATTGATGGG+TGG - chr7.2:80083105-80083124 MS.gene62016:intron 45.0%
ATGCCTCTTGGAATATGGGT+TGG - chr7.2:80083983-80084002 MS.gene62016:intron 45.0%
ATGTTAGTGACCTGAGGCAA+GGG + chr7.2:80083098-80083117 None:intergenic 45.0%
CCATCAATGTTAGTGACCTG+AGG + chr7.2:80083104-80083123 None:intergenic 45.0%
CCTCAGGTCACTAACATTGA+TGG - chr7.2:80083101-80083120 MS.gene62016:intron 45.0%
CGTTGTCATGTTCCAGATTG+TGG - chr7.2:80085417-80085436 MS.gene62016:CDS 45.0%
CTTTCTCGTCTCCATCGAAA+AGG + chr7.2:80085081-80085100 None:intergenic 45.0%
GACGAGGCATGTTCTTCAAT+AGG + chr7.2:80084796-80084815 None:intergenic 45.0%
GTGTCAGAAGAGAATCCATC+GGG - chr7.2:80083356-80083375 MS.gene62016:CDS 45.0%
GTTTGTCATCGTGGTGGTAT+TGG - chr7.2:80083398-80083417 MS.gene62016:CDS 45.0%
TACCTTTATAGCCCATGCGA+AGG + chr7.2:80084074-80084093 None:intergenic 45.0%
TCCCTCTTATAAACACCCGA+TGG + chr7.2:80083374-80083393 None:intergenic 45.0%
TGTCATGTTCCAGATTGTGG+TGG - chr7.2:80085420-80085439 MS.gene62016:CDS 45.0%
TTGTCGCAAAGCTGTCAAGT+TGG - chr7.2:80084308-80084327 MS.gene62016:CDS 45.0%
! GATGAGGGGATTCCTTTTGA+AGG - chr7.2:80083227-80083246 MS.gene62016:CDS 45.0%
! GGTCACTAACATTGATGGGT+GGG - chr7.2:80083106-80083125 MS.gene62016:intron 45.0%
! TCCACTTCAACAGTTTTGCG+TGG + chr7.2:80083428-80083447 None:intergenic 45.0%
! TGGCATTGAGGAAAAATGGG+TGG - chr7.2:80083517-80083536 MS.gene62016:intron 45.0%
! TTCCTTCGCATGGGCTATAA+AGG - chr7.2:80084069-80084088 MS.gene62016:CDS 45.0%
!! ATGGGTGGGTTCCTTTTGAA+GGG - chr7.2:80083120-80083139 MS.gene62016:intron 45.0%
AACGAGAACAGGTTGCCACA+AGG - chr7.2:80085208-80085227 MS.gene62016:CDS 50.0%
ACGAGAACAGGTTGCCACAA+GGG - chr7.2:80085209-80085228 MS.gene62016:CDS 50.0%
ATGACAACGACGAGGCTTCT+TGG + chr7.2:80085406-80085425 None:intergenic 50.0%
GCAGCAATTGAAGCTGCCAA+TGG - chr7.2:80084877-80084896 MS.gene62016:CDS 50.0%
! TCTGGAACATGACAACGACG+AGG + chr7.2:80085414-80085433 None:intergenic 50.0%
!! CTCACAGTTTGGTTTGGGTG+TGG - chr7.2:80084438-80084457 MS.gene62016:CDS 50.0%
!! GATGGGTGGGTTCCTTTTGA+AGG - chr7.2:80083119-80083138 MS.gene62016:intron 50.0%
! GCAGCTTCAACCCCTCCTTT+TGG + chr7.2:80085384-80085403 None:intergenic 55.0%
! TAGTGATTGGCCCTTGCCTC+AGG - chr7.2:80083085-80083104 MS.gene62016:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 80083059 80085625 80083059 ID=MS.gene62016
chr7.2 mRNA 80083059 80085625 80083059 ID=MS.gene62016.t1;Parent=MS.gene62016
chr7.2 exon 80084594 80085625 80084594 ID=MS.gene62016.t1.exon1;Parent=MS.gene62016.t1
chr7.2 CDS 80084594 80085625 80084594 ID=cds.MS.gene62016.t1;Parent=MS.gene62016.t1
chr7.2 exon 80084057 80084500 80084057 ID=MS.gene62016.t1.exon2;Parent=MS.gene62016.t1
chr7.2 CDS 80084057 80084500 80084057 ID=cds.MS.gene62016.t1;Parent=MS.gene62016.t1
chr7.2 exon 80083739 80083981 80083739 ID=MS.gene62016.t1.exon3;Parent=MS.gene62016.t1
chr7.2 CDS 80083739 80083981 80083739 ID=cds.MS.gene62016.t1;Parent=MS.gene62016.t1
chr7.2 exon 80083167 80083519 80083167 ID=MS.gene62016.t1.exon4;Parent=MS.gene62016.t1
chr7.2 CDS 80083167 80083519 80083167 ID=cds.MS.gene62016.t1;Parent=MS.gene62016.t1
chr7.2 exon 80083059 80083080 80083059 ID=MS.gene62016.t1.exon5;Parent=MS.gene62016.t1
chr7.2 CDS 80083059 80083080 80083059 ID=cds.MS.gene62016.t1;Parent=MS.gene62016.t1
Gene Sequence

>MS.gene62016

ATGATATCTGAAGTAAGCAATGATGCTAGTGATTGGCCCTTGCCTCAGGTCACTAACATTGATGGGTGGGTTCCTTTTGAAGGGCAAATATTTGAAGATGATGAATATGTAAATTCTTTAACATTTCAAGAGCTACAACATGAACCCTTGCCTCAAGTCAATAACATTGATGAGGGGATTCCTTTTGAAGGGAAAATATTTGAAAGTGATAATGCTGCTTTTGAATTTTATTGTTTATTTGCAAAACAAAATGGATTTTCTATTAGGCGTGATCACATTTATAAATCTTGTAAATTTGTGTCAGAAGAGAATCCATCGGGTGTTTATAAGAGGGAATTTGTTTGTCATCGTGGTGGTATTGGTAAACCACGCAAAACTGTTGAAGTGGAAAATCAAAGGAAACGAAAATCTTCAAGATGTGATTGTAGTGCAAAGATGGTAGTTACTAAGAGAAGAATTGGCATTGAGGAAAAATGGGTGGTTAAACATTTCAACAATTCTCACAACCACGAGTTATTGGATGACAAAGAGGTTCAATTTCTTCCTGCTTATCGTAACATTCCAATTGTTGATCAAGATCGCATACTATTATTGTCTAAAGCCGGTTGCCCTGTAAGTCTTATAGCGAGAGTGCTTGAGTTAGAGAAAAGAACAGGCACAGGAAATTTACCATTCTTAGAAAAAGATATTAGAAATTTTATTCAATCATATAGTGGTATTGGTAAAGAAAGTGATGCTTCAGATGTTCTCAGATTGTGCAAAAGTTTGAAAGATCGAGATGAGGCCTTCCAATATGAATTCACAATTGATGGAAGTAACAAATTGGAGCATATTGTTTGGGCATTTGGTGATTCAATTCGTGCATATGAAGCTTTTGGAGATGTTGTAGTTTTTGATACCACATATAGAATAAACCGTTATGAGATGCCTCTTGGAATATGGGTTGGTGTTGATAATCATGGAAATTCAATTTTTTTTGGTTGCGTTCTATTGCGAAATGAGAAGATTTCTTCCTTCGCATGGGCTATAAAGGTAAATTTCTATTTACTCAAAGTTGCTATCACTGTTAAATTTTTTAATTTGAAACTTTATTAAATTATTTTATATTTATGTTACTATTTTCAGAGTTTCCTAGCTTTTGTGAAAGGAAAATATCCAAAAACAATTCTTACCGATCAAGATCCTTCAATTAAAGAAGCCATTGCAATGGAATTGCCAAACACAAAACATGCATTTTGCATATGGCATATTGTCGCAAAGCTGTCAAGTTGGTTTTCTTTTCCACTAGGTTCGAGATATGATGACTTTAAACATGAGTTTCATAAGGTGTATCATTTAGAATGTGCAGATGATTTTGAACGAGAATGGAAAGTAATGGTCTCACAGTTTGGTTTGGGTGTGGATAAGCATATTGACTTGCTCTTTACCCATCGTAAATTTTGGGCCTTAGCTTATTTGAAGGACTTTTTTTTTGCTGGAATGACAACAACAGGACGCTCAGAATCGATAAATTCATATATAAAAAGATTCTTAGATGCCAAAACAAGTTTGACAGATTTTGTAAATCAAGTAAGAACTTACTTGATACATTTTTTTTAATCAATTAGCTTATAATTTAGTCATGATTGTTATATTTTTGTGTAGGTTGGTGTTGCTGTCAAAATTAGAAATCAAGCTGGAGAAGAGGCACGAATGCGTCAAAAGTATCATAATCCTCATATTACAACACATTTTCCTATTGAAGAACATGCCTCGTCTATACTCACACCTTATGCCTTTAAGTTGCTTCAACATGAGATCGAATTATCTGCAAAGTATGCAGCAATTGAAGCTGCCAATGGCTCTTATGTTGTGCAACATCATACCAAAGTTGATGGTGGTCGTATAGTAAGTTTGATAAAAGAAGAAGAGTCAATTCATTGTTCTTGTAAAGAGTTTCAATTTTCAGGAATAATATGTAGACATGCAATTCGAGTGCTAATGTTGAAAAATTATTTTGTTCTTCCATCAAAATATCTTCCTTTTCGATGGAGACGAGAAAGTTCATTGATCCCAAAATCTAGTCATGTAATAAACTGCAATGATGGATCCTCTGCTAAATTTCAGTCTCTAATTCGATGTTTGGAAGTCGAATCATTAAAGACAAGTGAACGAGAACAGGTTGCCACAAGGGAGTTAGAAAATATCATCATAAAGATAAAAGGCATGCCTGAAACTCAAGAACATGTGATTGATTTGGAAGAGGATGTTTCCAACAATGCTGACTGTGACGTTGAAAATCCTGTTGTTTCAAAAACAAAAGGTCGGCCAAAAGGATCAAGACCAAAAGGAGGGGTTGAAGCTGCCAAGAAGCCTCGTCGTTGTCATGTTCCAGATTGTGGTGGAACTAATCACGACTCACGAAACTGTCCAAACAAAAAGAGAAAAATGGAAGTGTTGCCTTCTCAATCTCCTAATAAGTAAGTTTTAATTGGTGTGTCTTGCTCTACTCATGAGTAATATATTGAATTGTAATATTAACTGTATTTTTATGGTGCTTTGATAGGATCATGAAGGGTTGGAAGTAG
Protein sequence

>MS.gene62016.t1

MISEVSNDASDWPLPQVTNIDGWVPFEGQIFEDDEYVNSLTFQELQHEPLPQVNNIDEGIPFEGKIFESDNAAFEFYCLFAKQNGFSIRRDHIYKSCKFVSEENPSGVYKREFVCHRGGIGKPRKTVEVENQRKRKSSRCDCSAKMVVTKRRIGIEEKWVVKHFNNSHNHELLDDKEVQFLPAYRNIPIVDQDRILLLSKAGCPVSLIARVLELEKRTGTGNLPFLEKDIRNFIQSYSGIGKESDASDVLRLCKSLKDRDEAFQYEFTIDGSNKLEHIVWAFGDSIRAYEAFGDVVVFDTTYRINRYEMPLGIWVGVDNHGNSIFFGCVLLRNEKISSFAWAIKSFLAFVKGKYPKTILTDQDPSIKEAIAMELPNTKHAFCIWHIVAKLSSWFSFPLGSRYDDFKHEFHKVYHLECADDFEREWKVMVSQFGLGVDKHIDLLFTHRKFWALAYLKDFFFAGMTTTGRSESINSYIKRFLDAKTSLTDFVNQVGVAVKIRNQAGEEARMRQKYHNPHITTHFPIEEHASSILTPYAFKLLQHEIELSAKYAAIEAANGSYVVQHHTKVDGGRISLIRCLEVESLKTSEREQVATRELENIIIKIKGMPETQEHVIDLEEDVSNNADCDVENPVVSKTKGRPKGSRPKGGVEAAKKPRRCHVPDCGGTNHDSRNCPNKKRKMEVLPSQSPNKIMKGWK