Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62053.t1 | XP_024634530.1 | 72.4 | 174 | 48 | 0 | 1 | 174 | 872 | 1045 | 1.70E-68 | 268.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62053.t1 | A0A392MKB5 | 66.1 | 174 | 59 | 0 | 1 | 174 | 181 | 354 | 5.0e-62 | 246.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene62053 | 0.801455 | 8.93E-49 | -1.69E-46 |
| MS.gene054174 | MS.gene62053 | 0.966308 | 9.52E-126 | -1.69E-46 |
| MS.gene054175 | MS.gene62053 | 0.976414 | 8.84E-142 | -1.69E-46 |
| MS.gene058515 | MS.gene62053 | 0.802715 | 4.92E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGAACTTCTTTATTTGC+TGG | - | 14564:16201-16220 | MS.gene62053:intergenic | 25.0% |
| ! | GAATCTCTCCTTTTTCTTAA+TGG | - | 14564:16149-16168 | MS.gene62053:intergenic | 30.0% |
| ACCCTGCTGACAAAATAAAT+TGG | - | 14564:16340-16359 | None:intergenic | 35.0% | |
| GAGTATTAAATCCCAAGTGT+TGG | + | 14564:16579-16598 | MS.gene62053:CDS | 35.0% | |
| TCAAATCAGATTACCCAATC+AGG | + | 14564:16467-16486 | MS.gene62053:CDS | 35.0% | |
| ! | ACTTTGTGACTTTGGAAGTA+GGG | - | 14564:16442-16461 | MS.gene62053:intergenic | 35.0% |
| ! | TCTCTCCTTTTTCTTAATGG+AGG | - | 14564:16146-16165 | MS.gene62053:intergenic | 35.0% |
| !!! | AGCCAATTTATTTTGTCAGC+AGG | + | 14564:16335-16354 | MS.gene62053:CDS | 35.0% |
| !!! | GCCAATTTATTTTGTCAGCA+GGG | + | 14564:16336-16355 | MS.gene62053:CDS | 35.0% |
| AGAAAAAGGAGAGATTCGAG+TGG | + | 14564:16152-16171 | MS.gene62053:CDS | 40.0% | |
| CTTCGCCTCCATTAAGAAAA+AGG | + | 14564:16138-16157 | MS.gene62053:CDS | 40.0% | |
| TACAAAGTCTGCCAACACTT+GGG | - | 14564:16593-16612 | MS.gene62053:intergenic | 40.0% | |
| TGGGATTTAATACTCCCTCT+TGG | - | 14564:16574-16593 | MS.gene62053:intergenic | 40.0% | |
| ! | CTTTGTGACTTTGGAAGTAG+GGG | - | 14564:16441-16460 | MS.gene62053:intergenic | 40.0% |
| ! | GACTTTGTGACTTTGGAAGT+AGG | - | 14564:16443-16462 | MS.gene62053:intergenic | 40.0% |
| ! | GTCATACTCTGAAAGCTCTA+TGG | - | 14564:16539-16558 | MS.gene62053:intergenic | 40.0% |
| AATGGAGGCGAAGAAAGAGA+AGG | - | 14564:16131-16150 | None:intergenic | 45.0% | |
| ACAGGAAAAGAATGGCGTTC+TGG | - | 14564:16252-16271 | None:intergenic | 45.0% | |
| CAGGAAAAGAATGGCGTTCT+GGG | - | 14564:16251-16270 | MS.gene62053:intergenic | 45.0% | |
| CTACAAAGTCTGCCAACACT+TGG | - | 14564:16594-16613 | MS.gene62053:intergenic | 45.0% | |
| TGACGACGACTTTGTGACTT+TGG | - | 14564:16450-16469 | MS.gene62053:intergenic | 45.0% | |
| TTCCCAGCACTTTCCTGATT+GGG | - | 14564:16483-16502 | MS.gene62053:intergenic | 45.0% | |
| TTGGTCGAAGATTCAGCAAC+AGG | + | 14564:16307-16326 | MS.gene62053:CDS | 45.0% | |
| TTTCCCAGCACTTTCCTGAT+TGG | - | 14564:16484-16503 | MS.gene62053:intergenic | 45.0% | |
| ! | AAAAGAATGGCGTTCTGGGT+TGG | - | 14564:16247-16266 | MS.gene62053:intergenic | 45.0% |
| ! | TTACCCAATCAGGAAAGTGC+TGG | + | 14564:16477-16496 | MS.gene62053:CDS | 45.0% |
| !! | TACCCAATCAGGAAAGTGCT+GGG | + | 14564:16478-16497 | MS.gene62053:CDS | 45.0% |
| AAAGGAGGTTGTTCCTCTGC+TGG | - | 14564:16631-16650 | None:intergenic | 50.0% | |
| AAGGAGGTTGTTCCTCTGCT+GGG | - | 14564:16630-16649 | None:intergenic | 50.0% | |
| ACGTCCAGTTCGTACCAAGA+GGG | + | 14564:16557-16576 | MS.gene62053:CDS | 50.0% | |
| ATCTGACAACGCCATGAGCA+CGG | + | 14564:16282-16301 | MS.gene62053:CDS | 50.0% | |
| CGCCAGGTACAGGAAAAGAA+TGG | - | 14564:16260-16279 | MS.gene62053:intergenic | 50.0% | |
| TACTCCCTCTTGGTACGAAC+TGG | - | 14564:16564-16583 | MS.gene62053:intergenic | 50.0% | |
| TGTCAGATATCGCCAGGTAC+AGG | - | 14564:16270-16289 | MS.gene62053:intergenic | 50.0% | |
| TTCGACCAAAACCGTGCTCA+TGG | - | 14564:16296-16315 | MS.gene62053:intergenic | 50.0% | |
| ! | AGTGCTGGGAAAACCTGACT+TGG | + | 14564:16492-16511 | MS.gene62053:CDS | 50.0% |
| ! | CGCCATTCTTTTCCTGTACC+TGG | + | 14564:16255-16274 | MS.gene62053:CDS | 50.0% |
| ! | CTCTGAAAGCTCTATGGACC+AGG | - | 14564:16533-16552 | MS.gene62053:intergenic | 50.0% |
| ! | TTTGTCAGCAGGGTGTTCAG+AGG | + | 14564:16346-16365 | MS.gene62053:CDS | 50.0% |
| !! | TGGAAAGCGTCCTCACATTC+TGG | - | 14564:16181-16200 | MS.gene62053:intergenic | 50.0% |
| !! | TGGGAAAACCTGACTTGGCA+GGG | + | 14564:16497-16516 | MS.gene62053:CDS | 50.0% |
| ACCATTCTCCCTGCCAAGTC+AGG | - | 14564:16508-16527 | None:intergenic | 55.0% | |
| AGGAGGTTGTTCCTCTGCTG+GGG | - | 14564:16629-16648 | MS.gene62053:intergenic | 55.0% | |
| CGCCCTCGCTGTCATCATAA+CGG | + | 14564:16402-16421 | MS.gene62053:CDS | 55.0% | |
| GACGTCCAGTTCGTACCAAG+AGG | + | 14564:16556-16575 | MS.gene62053:CDS | 55.0% | |
| GGCCGTTATGATGACAGCGA+GGG | - | 14564:16407-16426 | MS.gene62053:intergenic | 55.0% | |
| TCGCTGTCATCATAACGGCC+AGG | + | 14564:16407-16426 | MS.gene62053:CDS | 55.0% | |
| TGGCCGTTATGATGACAGCG+AGG | - | 14564:16408-16427 | MS.gene62053:intergenic | 55.0% | |
| TGGCGTTGTCAGATATCGCC+AGG | - | 14564:16276-16295 | MS.gene62053:intergenic | 55.0% | |
| ! | CAACGCCATGAGCACGGTTT+TGG | + | 14564:16288-16307 | MS.gene62053:CDS | 55.0% |
| ! | CGAGTGGACTCCAGAATGTG+AGG | + | 14564:16168-16187 | MS.gene62053:CDS | 55.0% |
| ! | TGACTTGGCAGGGAGAATGG+TGG | + | 14564:16507-16526 | MS.gene62053:CDS | 55.0% |
| !! | ACCTGACTTGGCAGGGAGAA+TGG | + | 14564:16504-16523 | MS.gene62053:CDS | 55.0% |
| !! | CTGGGAAAACCTGACTTGGC+AGG | + | 14564:16496-16515 | MS.gene62053:CDS | 55.0% |
| !! | TGGGTTGGTCGCTGAAGGAT+GGG | - | 14564:16232-16251 | MS.gene62053:intergenic | 55.0% |
| AGGTTGTTCCTCTGCTGGGG+AGG | - | 14564:16626-16645 | MS.gene62053:intergenic | 60.0% | |
| GAAGTAGGGGCGAAGCTTCC+TGG | - | 14564:16428-16447 | MS.gene62053:intergenic | 60.0% | |
| GTTGGTCGCTGAAGGATGGG+CGG | - | 14564:16229-16248 | MS.gene62053:intergenic | 60.0% | |
| !! | CGTTCTGGGTTGGTCGCTGA+AGG | - | 14564:16237-16256 | MS.gene62053:intergenic | 60.0% |
| !! | CTGGGTTGGTCGCTGAAGGA+TGG | - | 14564:16233-16252 | MS.gene62053:intergenic | 60.0% |
| AGAACTCGCCTCCCCAGCAG+AGG | + | 14564:16615-16634 | MS.gene62053:CDS | 65.0% | |
| TCGCTGAAGGATGGGCGGAC+TGG | - | 14564:16224-16243 | MS.gene62053:intergenic | 65.0% | |
| TGGCAGGGAGAATGGTGGCC+TGG | + | 14564:16512-16531 | MS.gene62053:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 14564 | gene | 16126 | 16651 | 16126 | ID=MS.gene62053 |
| 14564 | mRNA | 16126 | 16651 | 16126 | ID=MS.gene62053.t1;Parent=MS.gene62053 |
| 14564 | exon | 16126 | 16651 | 16126 | ID=MS.gene62053.t1.exon1;Parent=MS.gene62053.t1 |
| 14564 | CDS | 16126 | 16651 | 16126 | ID=cds.MS.gene62053.t1;Parent=MS.gene62053.t1 |
| Gene Sequence |
| Protein sequence |
