Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62080.t1 | XP_003610423.1 | 93.2 | 132 | 9 | 0 | 1 | 132 | 45 | 176 | 3.60E-63 | 250.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62080.t1 | G7JJ63 | 93.2 | 132 | 9 | 0 | 1 | 132 | 45 | 176 | 2.6e-63 | 250.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049396 | MS.gene62080 | 0.817776 | 2.77E-52 | -1.69E-46 |
| MS.gene050697 | MS.gene62080 | 0.830157 | 3.44E-55 | -1.69E-46 |
| MS.gene05494 | MS.gene62080 | 0.818835 | 1.59E-52 | -1.69E-46 |
| MS.gene056387 | MS.gene62080 | 0.842796 | 2.08E-58 | -1.69E-46 |
| MS.gene06097 | MS.gene62080 | 0.804583 | 2.01E-49 | -1.69E-46 |
| MS.gene061218 | MS.gene62080 | 0.970493 | 1.06E-131 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCGCAAGCGTTTGAGAATC+AGG | 0.306206 | 8.2:-2422293 | MS.gene62080:CDS |
| CAATTTCTCTCCCGGCGATT+GGG | 0.307565 | 8.2:-2422356 | MS.gene62080:CDS |
| GGTTCCGCCGCATGAGTATT+TGG | 0.311065 | 8.2:-2422113 | MS.gene62080:CDS |
| TTTGGCGCGTGGCCGGAAAA+TGG | 0.357971 | 8.2:-2422095 | MS.gene62080:CDS |
| CCGGAAAATGGCGGCAAATT+CGG | 0.361903 | 8.2:-2422083 | MS.gene62080:CDS |
| GGTGGAATCGTTGCATGATA+TGG | 0.383426 | 8.2:-2422158 | MS.gene62080:CDS |
| GGGGTGGGTCGGACCTTAAA+AGG | 0.389495 | 8.2:-2422052 | MS.gene62080:CDS |
| GTGCTGATGTTGCAACGTTC+CGG | 0.398619 | 8.2:+2422224 | None:intergenic |
| TCAATTTCTCTCCCGGCGAT+TGG | 0.413239 | 8.2:-2422357 | MS.gene62080:CDS |
| GACGGGTTCGTGATGCTGTT+TGG | 0.413527 | 8.2:-2422018 | MS.gene62080:CDS |
| CTCCAATCCGGCACGTTCAC+CGG | 0.413829 | 8.2:+2422202 | None:intergenic |
| CTCAACCATCGAACCCGGTT+TGG | 0.416497 | 8.2:+2421993 | None:intergenic |
| GATGCTGTTTGGAACCAAAC+CGG | 0.441060 | 8.2:-2422007 | MS.gene62080:CDS |
| ATGTTGCAACGTTCCGGTAC+TGG | 0.447319 | 8.2:+2422230 | None:intergenic |
| CACCGGTGAACGTGCCGGAT+TGG | 0.454006 | 8.2:-2422204 | MS.gene62080:CDS |
| GAATCGTTCACCACCAGTAC+CGG | 0.485009 | 8.2:-2422243 | MS.gene62080:CDS |
| ATGAGTATTTGGCGCGTGGC+CGG | 0.490622 | 8.2:-2422102 | MS.gene62080:CDS |
| ATGCTGTTTGGAACCAAACC+GGG | 0.500659 | 8.2:-2422006 | MS.gene62080:CDS |
| CGGAGTATCTTACTCCAATC+CGG | 0.504693 | 8.2:+2422190 | None:intergenic |
| TAAGATACTCCGAGTTGAAT+CGG | 0.506960 | 8.2:-2422179 | MS.gene62080:CDS |
| TGCGCGTGAATCCCAATCGC+CGG | 0.518977 | 8.2:+2422345 | None:intergenic |
| GATTCTCAAACGCTTGCGAC+AGG | 0.525300 | 8.2:+2422296 | None:intergenic |
| AAAAGGCCGTGATTTGAGAC+GGG | 0.530895 | 8.2:-2422035 | MS.gene62080:CDS |
| TATGGATGATGATAACGAAT+CGG | 0.540008 | 8.2:-2422140 | MS.gene62080:CDS |
| TGGAACCAAACCGGGTTCGA+TGG | 0.549508 | 8.2:-2421998 | MS.gene62080:CDS |
| CACAACGTCAATTTCTCTCC+CGG | 0.549711 | 8.2:-2422364 | MS.gene62080:CDS |
| TAAAAGGCCGTGATTTGAGA+CGG | 0.552056 | 8.2:-2422036 | MS.gene62080:CDS |
| GCGCGTGAATCCCAATCGCC+GGG | 0.553493 | 8.2:+2422346 | None:intergenic |
| ACGCTTGCGACAGGCCGCCG+TGG | 0.563075 | 8.2:+2422305 | None:intergenic |
| CACGAACCCGTCTCAAATCA+CGG | 0.568944 | 8.2:+2422029 | None:intergenic |
| TGATGATAACGAATCGGAGA+TGG | 0.569605 | 8.2:-2422134 | MS.gene62080:CDS |
| CAAACCGGGTTCGATGGTTG+AGG | 0.576026 | 8.2:-2421992 | None:intergenic |
| CGCGCCAAATACTCATGCGG+CGG | 0.577795 | 8.2:+2422109 | None:intergenic |
| ATCAGCACCGGTGAACGTGC+CGG | 0.584228 | 8.2:-2422209 | MS.gene62080:CDS |
| TCCACCTCAACCATCGAACC+CGG | 0.586804 | 8.2:+2421988 | None:intergenic |
| GGCGCGTGGCCGGAAAATGG+CGG | 0.591649 | 8.2:-2422092 | MS.gene62080:CDS |
| CCGCATGAGTATTTGGCGCG+TGG | 0.607155 | 8.2:-2422106 | MS.gene62080:CDS |
| GAACGTTGCAACATCAGCAC+CGG | 0.627010 | 8.2:-2422221 | MS.gene62080:CDS |
| ACGATTCCACCGATTCAACT+CGG | 0.637845 | 8.2:+2422170 | None:intergenic |
| CCACGCGCCAAATACTCATG+CGG | 0.647845 | 8.2:+2422106 | None:intergenic |
| CATTCACGTCGTCACAACCA+CGG | 0.652333 | 8.2:-2422322 | MS.gene62080:CDS |
| CGGGAGAGAAATTGACGTTG+TGG | 0.653823 | 8.2:+2422365 | None:intergenic |
| TTGCAACGTTCCGGTACTGG+TGG | 0.660475 | 8.2:+2422233 | None:intergenic |
| GATACTCCGAGTTGAATCGG+TGG | 0.676763 | 8.2:-2422176 | MS.gene62080:CDS |
| TCACGTCGTCACAACCACGG+CGG | 0.789082 | 8.2:-2422319 | MS.gene62080:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATGGATGATGATAACGAAT+CGG | - | chr8.2:2422226-2422245 | MS.gene62080:CDS | 30.0% |
| ! | TAAGATACTCCGAGTTGAAT+CGG | - | chr8.2:2422187-2422206 | MS.gene62080:CDS | 35.0% |
| TAAAAGGCCGTGATTTGAGA+CGG | - | chr8.2:2422330-2422349 | MS.gene62080:CDS | 40.0% | |
| ! | TCTCAAATCACGGCCTTTTA+AGG | + | chr8.2:2422330-2422349 | None:intergenic | 40.0% |
| ! | TGATGATAACGAATCGGAGA+TGG | - | chr8.2:2422232-2422251 | MS.gene62080:CDS | 40.0% |
| CACAACGTCAATTTCTCTCC+CGG | - | chr8.2:2422002-2422021 | MS.gene62080:CDS | 45.0% | |
| GGTGGAATCGTTGCATGATA+TGG | - | chr8.2:2422208-2422227 | MS.gene62080:CDS | 45.0% | |
| ! | AAAAGGCCGTGATTTGAGAC+GGG | - | chr8.2:2422331-2422350 | MS.gene62080:CDS | 45.0% |
| ! | AAATTCGGTTTTCGAAGGGG+TGG | - | chr8.2:2422298-2422317 | MS.gene62080:CDS | 45.0% |
| ! | AATTCGGTTTTCGAAGGGGT+GGG | - | chr8.2:2422299-2422318 | MS.gene62080:CDS | 45.0% |
| ! | ATGCTGTTTGGAACCAAACC+GGG | - | chr8.2:2422360-2422379 | MS.gene62080:CDS | 45.0% |
| ! | CGGAGTATCTTACTCCAATC+CGG | + | chr8.2:2422179-2422198 | None:intergenic | 45.0% |
| ! | CGGCAAATTCGGTTTTCGAA+GGG | - | chr8.2:2422294-2422313 | MS.gene62080:CDS | 45.0% |
| ! | GATGCTGTTTGGAACCAAAC+CGG | - | chr8.2:2422359-2422378 | MS.gene62080:CDS | 45.0% |
| ! | GGCAAATTCGGTTTTCGAAG+GGG | - | chr8.2:2422295-2422314 | MS.gene62080:CDS | 45.0% |
| !! | ACGATTCCACCGATTCAACT+CGG | + | chr8.2:2422199-2422218 | None:intergenic | 45.0% |
| ATGTTGCAACGTTCCGGTAC+TGG | + | chr8.2:2422139-2422158 | None:intergenic | 50.0% | |
| CAATTTCTCTCCCGGCGATT+GGG | - | chr8.2:2422010-2422029 | MS.gene62080:CDS | 50.0% | |
| CACGAACCCGTCTCAAATCA+CGG | + | chr8.2:2422340-2422359 | None:intergenic | 50.0% | |
| CATTCACGTCGTCACAACCA+CGG | - | chr8.2:2422044-2422063 | MS.gene62080:CDS | 50.0% | |
| CCGGAAAATGGCGGCAAATT+CGG | - | chr8.2:2422283-2422302 | MS.gene62080:CDS | 50.0% | |
| CGGGAGAGAAATTGACGTTG+TGG | + | chr8.2:2422004-2422023 | None:intergenic | 50.0% | |
| GAACGTTGCAACATCAGCAC+CGG | - | chr8.2:2422145-2422164 | MS.gene62080:CDS | 50.0% | |
| GAATCGTTCACCACCAGTAC+CGG | - | chr8.2:2422123-2422142 | MS.gene62080:CDS | 50.0% | |
| GATTCTCAAACGCTTGCGAC+AGG | + | chr8.2:2422073-2422092 | None:intergenic | 50.0% | |
| GTCGCAAGCGTTTGAGAATC+AGG | - | chr8.2:2422073-2422092 | MS.gene62080:CDS | 50.0% | |
| TCAATTTCTCTCCCGGCGAT+TGG | - | chr8.2:2422009-2422028 | MS.gene62080:CDS | 50.0% | |
| ! | GATACTCCGAGTTGAATCGG+TGG | - | chr8.2:2422190-2422209 | MS.gene62080:CDS | 50.0% |
| ! | GCGGCAAATTCGGTTTTCGA+AGG | - | chr8.2:2422293-2422312 | MS.gene62080:CDS | 50.0% |
| ! | GTGCTGATGTTGCAACGTTC+CGG | + | chr8.2:2422145-2422164 | None:intergenic | 50.0% |
| !! | CCGAATTTGCCGCCATTTTC+CGG | + | chr8.2:2422286-2422305 | None:intergenic | 50.0% |
| ATGAGTATTTGGCGCGTGGC+CGG | - | chr8.2:2422264-2422283 | MS.gene62080:CDS | 55.0% | |
| CCACGCGCCAAATACTCATG+CGG | + | chr8.2:2422263-2422282 | None:intergenic | 55.0% | |
| GGTTCCGCCGCATGAGTATT+TGG | - | chr8.2:2422253-2422272 | MS.gene62080:CDS | 55.0% | |
| TGGAACCAAACCGGGTTCGA+TGG | - | chr8.2:2422368-2422387 | MS.gene62080:CDS | 55.0% | |
| ! | GACGGGTTCGTGATGCTGTT+TGG | - | chr8.2:2422348-2422367 | MS.gene62080:CDS | 55.0% |
| ! | TTGCAACGTTCCGGTACTGG+TGG | + | chr8.2:2422136-2422155 | None:intergenic | 55.0% |
| CCGCATGAGTATTTGGCGCG+TGG | - | chr8.2:2422260-2422279 | MS.gene62080:CDS | 60.0% | |
| CGCGCCAAATACTCATGCGG+CGG | + | chr8.2:2422260-2422279 | None:intergenic | 60.0% | |
| CTCCAATCCGGCACGTTCAC+CGG | + | chr8.2:2422167-2422186 | None:intergenic | 60.0% | |
| GGGGTGGGTCGGACCTTAAA+AGG | - | chr8.2:2422314-2422333 | MS.gene62080:CDS | 60.0% | |
| TCACGTCGTCACAACCACGG+CGG | - | chr8.2:2422047-2422066 | MS.gene62080:CDS | 60.0% | |
| TGCGCGTGAATCCCAATCGC+CGG | + | chr8.2:2422024-2422043 | None:intergenic | 60.0% | |
| TTTGGCGCGTGGCCGGAAAA+TGG | - | chr8.2:2422271-2422290 | MS.gene62080:CDS | 60.0% | |
| ! | CGGTTTTCGAAGGGGTGGGT+CGG | - | chr8.2:2422303-2422322 | MS.gene62080:CDS | 60.0% |
| !! | ATCAGCACCGGTGAACGTGC+CGG | - | chr8.2:2422157-2422176 | MS.gene62080:CDS | 60.0% |
| CACCGGTGAACGTGCCGGAT+TGG | - | chr8.2:2422162-2422181 | MS.gene62080:CDS | 65.0% | |
| GCGCGTGAATCCCAATCGCC+GGG | + | chr8.2:2422023-2422042 | None:intergenic | 65.0% | |
| GGCGCGTGGCCGGAAAATGG+CGG | - | chr8.2:2422274-2422293 | MS.gene62080:CDS | 70.0% | |
| ACGCTTGCGACAGGCCGCCG+TGG | + | chr8.2:2422064-2422083 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 2421994 | 2422394 | 2421994 | ID=MS.gene62080 |
| chr8.2 | mRNA | 2421994 | 2422394 | 2421994 | ID=MS.gene62080.t1;Parent=MS.gene62080 |
| chr8.2 | exon | 2421994 | 2422394 | 2421994 | ID=MS.gene62080.t1.exon1;Parent=MS.gene62080.t1 |
| chr8.2 | CDS | 2421994 | 2422394 | 2421994 | ID=cds.MS.gene62080.t1;Parent=MS.gene62080.t1 |
| Gene Sequence |
| Protein sequence |
