AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061218


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061218.t1 MTR_4g132020 93.750 176 11 0 1 176 1 176 1.28e-120 338
MS.gene061218.t1 MTR_2g023070 51.142 219 63 7 1 176 1 218 1.72e-55 174
MS.gene061218.t1 MTR_7g101250 37.705 183 82 8 1 175 1 159 9.18e-21 84.0
MS.gene061218.t1 MTR_2g094410 55.952 84 30 2 97 174 72 154 5.01e-20 82.0
MS.gene061218.t1 MTR_4g059320 51.190 84 35 2 96 174 75 157 6.05e-18 76.6
MS.gene061218.t1 MTR_2g023390 50.602 83 36 3 97 174 72 154 1.78e-16 72.8
MS.gene061218.t1 MTR_2g086660 48.810 84 35 3 99 174 170 253 1.70e-15 72.0
MS.gene061218.t1 MTR_2g086660 48.193 83 35 3 99 173 170 252 1.35e-14 70.1
MS.gene061218.t1 MTR_3g052330 28.415 183 83 5 32 174 22 196 4.03e-14 67.4
MS.gene061218.t1 MTR_7g105130 65.217 46 13 1 132 174 170 215 7.70e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061218.t1 AT1G11700 47.087 206 74 7 1 176 1 201 2.96e-45 148
MS.gene061218.t1 AT1G61930 48.529 204 76 9 1 176 1 203 4.11e-43 142
MS.gene061218.t1 AT4G21930 46.193 197 71 9 1 176 1 183 3.28e-38 129
MS.gene061218.t1 AT3G45210 56.757 74 30 2 101 174 75 146 8.07e-21 84.0
MS.gene061218.t1 AT5G60680 35.519 183 87 4 1 174 1 161 1.62e-19 80.9
MS.gene061218.t1 AT4G21970 47.059 85 37 3 98 174 59 143 8.31e-19 79.0
MS.gene061218.t1 AT5G03230 46.591 88 34 2 99 174 78 164 2.82e-17 75.1
MS.gene061218.t1 AT2G28400 48.889 90 35 3 96 175 73 161 3.90e-17 74.7
MS.gene061218.t1 AT4G04630 48.315 89 34 3 98 174 78 166 1.22e-14 68.2
MS.gene061218.t1 AT4G26950 34.146 164 76 8 21 174 1 142 9.05e-14 65.5
MS.gene061218.t1 AT4G26950 34.146 164 76 8 21 174 1 142 9.05e-14 65.5
MS.gene061218.t1 AT3G15040 59.184 49 17 1 129 174 193 241 3.42e-12 63.2
MS.gene061218.t1 AT1G29640 47.561 82 31 4 97 174 64 137 7.10e-12 60.5

Find 62 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATGAAGTAACCGATTTC+AGG 0.232378 8.2:+2388498 MS.gene061218:CDS
GTCGCAAGCGTTTGAGAATC+AGG 0.306206 8.2:+2388628 MS.gene061218:CDS
CAATTTCTCTCCCGGCGATT+GGG 0.307565 8.2:+2388565 MS.gene061218:CDS
GGTTCCGCCGCATGAGTATT+TGG 0.311065 8.2:+2388808 MS.gene061218:CDS
TTTGGCGCGTGGCCGGAAAA+TGG 0.357971 8.2:+2388826 MS.gene061218:CDS
CCGGAAAATGGCGGCAAATT+CGG 0.361903 8.2:+2388838 MS.gene061218:CDS
CACATCTTCTTCCCTGAAAT+CGG 0.365661 8.2:-2388510 None:intergenic
AACCGAAACGAACGGTTGTT+AGG 0.366911 8.2:+2388446 MS.gene061218:CDS
CAACCGTTCGTTTCGGTTGG+TGG 0.379141 8.2:-2388441 None:intergenic
GGTGGAATCGTTGCATGATA+TGG 0.383426 8.2:+2388763 MS.gene061218:CDS
GGGGTGGGTCGGACCTTAAA+AGG 0.389495 8.2:+2388869 MS.gene061218:CDS
TTCCTAACAACCGTTCGTTT+CGG 0.395593 8.2:-2388448 None:intergenic
GTGCTGATGTTGCAACGTTC+CGG 0.398619 8.2:-2388697 None:intergenic
TCAATTTCTCTCCCGGCGAT+TGG 0.413239 8.2:+2388564 MS.gene061218:CDS
GACGGGTTCGTGATGCTGTT+TGG 0.413527 8.2:+2388903 MS.gene061218:CDS
CTCCAATCCGGCACGTTCAC+CGG 0.413829 8.2:-2388719 None:intergenic
CTCAACCATCGAACCCGGTT+TGG 0.416497 8.2:-2388928 None:intergenic
GATGCTGTTTGGAACCAAAC+CGG 0.441060 8.2:+2388914 MS.gene061218:CDS
ATGTTGCAACGTTCCGGTAC+TGG 0.447319 8.2:-2388691 None:intergenic
CACCGGTGAACGTGCCGGAT+TGG 0.454006 8.2:+2388717 MS.gene061218:CDS
GAATCGTTCACCACCAGTAC+CGG 0.485009 8.2:+2388678 MS.gene061218:CDS
ATGAGTATTTGGCGCGTGGC+CGG 0.490622 8.2:+2388819 MS.gene061218:CDS
ATGCTGTTTGGAACCAAACC+GGG 0.500659 8.2:+2388915 MS.gene061218:CDS
CGGAGTATCTTACTCCAATC+CGG 0.504693 8.2:-2388731 None:intergenic
TAAGATACTCCGAGTTGAAT+CGG 0.506960 8.2:+2388742 MS.gene061218:CDS
TCATGAAGTAACCGATTTCA+GGG 0.508081 8.2:+2388499 MS.gene061218:CDS
TAACAACCGTTCGTTTCGGT+TGG 0.515395 8.2:-2388444 None:intergenic
TGCGCGTGAATCCCAATCGC+CGG 0.518977 8.2:-2388576 None:intergenic
GATTCTCAAACGCTTGCGAC+AGG 0.525300 8.2:-2388625 None:intergenic
AAAAGGCCGTGATTTGAGAC+GGG 0.530895 8.2:+2388886 MS.gene061218:CDS
TATGGATGATGATAACGAAT+CGG 0.540008 8.2:+2388781 MS.gene061218:CDS
TGGAACCAAACCGGGTTCGA+TGG 0.549508 8.2:+2388923 MS.gene061218:CDS
CACAACGTCAATTTCTCTCC+CGG 0.549711 8.2:+2388557 MS.gene061218:CDS
TAAAAGGCCGTGATTTGAGA+CGG 0.552056 8.2:+2388885 MS.gene061218:CDS
GCGCGTGAATCCCAATCGCC+GGG 0.553493 8.2:-2388575 None:intergenic
GGTTACTTCATGAGTGGTGG+TGG 0.557686 8.2:-2388489 None:intergenic
ACGCTTGCGACAGGCCGCCG+TGG 0.563075 8.2:-2388616 None:intergenic
GTCGCTAAGTACCATGGCGA+AGG 0.565479 8.2:+2388406 None:intergenic
CACGAACCCGTCTCAAATCA+CGG 0.568944 8.2:-2388892 None:intergenic
TGATGATAACGAATCGGAGA+TGG 0.569605 8.2:+2388787 MS.gene061218:CDS
CAAACCGGGTTCGATGGTTG+AGG 0.576026 8.2:+2388929 MS.gene061218:CDS
GTGGTCACGATCTTCAACCA+TGG 0.577498 8.2:-2388537 None:intergenic
CGCGCCAAATACTCATGCGG+CGG 0.577795 8.2:-2388812 None:intergenic
ATCAGCACCGGTGAACGTGC+CGG 0.584228 8.2:+2388712 MS.gene061218:CDS
TCCACCTCAACCATCGAACC+CGG 0.586804 8.2:-2388933 None:intergenic
GGCGCGTGGCCGGAAAATGG+CGG 0.591649 8.2:+2388829 MS.gene061218:CDS
GAATTTGCGACCCTTCGCCA+TGG 0.599300 8.2:-2388417 None:intergenic
CCGCATGAGTATTTGGCGCG+TGG 0.607155 8.2:+2388815 MS.gene061218:CDS
GAACGTTGCAACATCAGCAC+CGG 0.627010 8.2:+2388700 MS.gene061218:CDS
GGAAGAAGATGTGTGGTCCA+TGG 0.635452 8.2:+2388520 MS.gene061218:CDS
ACGATTCCACCGATTCAACT+CGG 0.637845 8.2:-2388751 None:intergenic
TCGCTAAGTACCATGGCGAA+GGG 0.643376 8.2:+2388407 None:intergenic
CCACGCGCCAAATACTCATG+CGG 0.647845 8.2:-2388815 None:intergenic
ATCGGTTACTTCATGAGTGG+TGG 0.648620 8.2:-2388492 None:intergenic
CATTCACGTCGTCACAACCA+CGG 0.652333 8.2:+2388599 MS.gene061218:CDS
CGGGAGAGAAATTGACGTTG+TGG 0.653823 8.2:-2388556 None:intergenic
TCACCACCAACCGAAACGAA+CGG 0.658770 8.2:+2388438 MS.gene061218:CDS
TTGCAACGTTCCGGTACTGG+TGG 0.660475 8.2:-2388688 None:intergenic
GATACTCCGAGTTGAATCGG+TGG 0.676763 8.2:+2388745 MS.gene061218:CDS
GAAATCGGTTACTTCATGAG+TGG 0.682994 8.2:-2388495 None:intergenic
ATTTCAGGGAAGAAGATGTG+TGG 0.693930 8.2:+2388513 MS.gene061218:CDS
TCACGTCGTCACAACCACGG+CGG 0.789082 8.2:+2388602 MS.gene061218:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATGGATGATGATAACGAAT+CGG + chr8.2:2388781-2388800 MS.gene061218:CDS 30.0%
! TAAGATACTCCGAGTTGAAT+CGG + chr8.2:2388742-2388761 MS.gene061218:CDS 35.0%
! TCATGAAGTAACCGATTTCA+GGG + chr8.2:2388499-2388518 MS.gene061218:CDS 35.0%
ATTTCAGGGAAGAAGATGTG+TGG + chr8.2:2388513-2388532 MS.gene061218:CDS 40.0%
CACATCTTCTTCCCTGAAAT+CGG - chr8.2:2388513-2388532 None:intergenic 40.0%
GAAATCGGTTACTTCATGAG+TGG - chr8.2:2388498-2388517 None:intergenic 40.0%
TAAAAGGCCGTGATTTGAGA+CGG + chr8.2:2388885-2388904 MS.gene061218:CDS 40.0%
TTCCTAACAACCGTTCGTTT+CGG - chr8.2:2388451-2388470 None:intergenic 40.0%
! CTCATGAAGTAACCGATTTC+AGG + chr8.2:2388498-2388517 MS.gene061218:CDS 40.0%
! TCTCAAATCACGGCCTTTTA+AGG - chr8.2:2388885-2388904 None:intergenic 40.0%
! TGATGATAACGAATCGGAGA+TGG + chr8.2:2388787-2388806 MS.gene061218:CDS 40.0%
AACCGAAACGAACGGTTGTT+AGG + chr8.2:2388446-2388465 MS.gene061218:CDS 45.0%
ATCGGTTACTTCATGAGTGG+TGG - chr8.2:2388495-2388514 None:intergenic 45.0%
CACAACGTCAATTTCTCTCC+CGG + chr8.2:2388557-2388576 MS.gene061218:CDS 45.0%
GGTGGAATCGTTGCATGATA+TGG + chr8.2:2388763-2388782 MS.gene061218:CDS 45.0%
TAACAACCGTTCGTTTCGGT+TGG - chr8.2:2388447-2388466 None:intergenic 45.0%
! AAAAGGCCGTGATTTGAGAC+GGG + chr8.2:2388886-2388905 MS.gene061218:CDS 45.0%
! AAATTCGGTTTTCGAAGGGG+TGG + chr8.2:2388853-2388872 MS.gene061218:CDS 45.0%
! AATTCGGTTTTCGAAGGGGT+GGG + chr8.2:2388854-2388873 MS.gene061218:CDS 45.0%
! ATGCTGTTTGGAACCAAACC+GGG + chr8.2:2388915-2388934 MS.gene061218:CDS 45.0%
! CGGAGTATCTTACTCCAATC+CGG - chr8.2:2388734-2388753 None:intergenic 45.0%
! CGGCAAATTCGGTTTTCGAA+GGG + chr8.2:2388849-2388868 MS.gene061218:CDS 45.0%
! GATGCTGTTTGGAACCAAAC+CGG + chr8.2:2388914-2388933 MS.gene061218:CDS 45.0%
! GGCAAATTCGGTTTTCGAAG+GGG + chr8.2:2388850-2388869 MS.gene061218:CDS 45.0%
!! ACGATTCCACCGATTCAACT+CGG - chr8.2:2388754-2388773 None:intergenic 45.0%
ATGTTGCAACGTTCCGGTAC+TGG - chr8.2:2388694-2388713 None:intergenic 50.0%
CAATTTCTCTCCCGGCGATT+GGG + chr8.2:2388565-2388584 MS.gene061218:CDS 50.0%
CACGAACCCGTCTCAAATCA+CGG - chr8.2:2388895-2388914 None:intergenic 50.0%
CATTCACGTCGTCACAACCA+CGG + chr8.2:2388599-2388618 MS.gene061218:CDS 50.0%
CCGGAAAATGGCGGCAAATT+CGG + chr8.2:2388838-2388857 MS.gene061218:CDS 50.0%
CGGGAGAGAAATTGACGTTG+TGG - chr8.2:2388559-2388578 None:intergenic 50.0%
GAACGTTGCAACATCAGCAC+CGG + chr8.2:2388700-2388719 MS.gene061218:CDS 50.0%
GAATCGTTCACCACCAGTAC+CGG + chr8.2:2388678-2388697 MS.gene061218:CDS 50.0%
GATTCTCAAACGCTTGCGAC+AGG - chr8.2:2388628-2388647 None:intergenic 50.0%
GGAAGAAGATGTGTGGTCCA+TGG + chr8.2:2388520-2388539 MS.gene061218:CDS 50.0%
GGTTACTTCATGAGTGGTGG+TGG - chr8.2:2388492-2388511 None:intergenic 50.0%
GTCGCAAGCGTTTGAGAATC+AGG + chr8.2:2388628-2388647 MS.gene061218:CDS 50.0%
GTGGTCACGATCTTCAACCA+TGG - chr8.2:2388540-2388559 None:intergenic 50.0%
TCAATTTCTCTCCCGGCGAT+TGG + chr8.2:2388564-2388583 MS.gene061218:CDS 50.0%
TCACCACCAACCGAAACGAA+CGG + chr8.2:2388438-2388457 MS.gene061218:CDS 50.0%
! GATACTCCGAGTTGAATCGG+TGG + chr8.2:2388745-2388764 MS.gene061218:CDS 50.0%
! GCGGCAAATTCGGTTTTCGA+AGG + chr8.2:2388848-2388867 MS.gene061218:CDS 50.0%
! GTGCTGATGTTGCAACGTTC+CGG - chr8.2:2388700-2388719 None:intergenic 50.0%
!! CCGAATTTGCCGCCATTTTC+CGG - chr8.2:2388841-2388860 None:intergenic 50.0%
ATGAGTATTTGGCGCGTGGC+CGG + chr8.2:2388819-2388838 MS.gene061218:CDS 55.0%
CAACCGTTCGTTTCGGTTGG+TGG - chr8.2:2388444-2388463 None:intergenic 55.0%
CCACGCGCCAAATACTCATG+CGG - chr8.2:2388818-2388837 None:intergenic 55.0%
GGTTCCGCCGCATGAGTATT+TGG + chr8.2:2388808-2388827 MS.gene061218:CDS 55.0%
TGGAACCAAACCGGGTTCGA+TGG + chr8.2:2388923-2388942 MS.gene061218:CDS 55.0%
! GACGGGTTCGTGATGCTGTT+TGG + chr8.2:2388903-2388922 MS.gene061218:CDS 55.0%
! TTGCAACGTTCCGGTACTGG+TGG - chr8.2:2388691-2388710 None:intergenic 55.0%
CCGCATGAGTATTTGGCGCG+TGG + chr8.2:2388815-2388834 MS.gene061218:CDS 60.0%
CGCGCCAAATACTCATGCGG+CGG - chr8.2:2388815-2388834 None:intergenic 60.0%
CTCCAATCCGGCACGTTCAC+CGG - chr8.2:2388722-2388741 None:intergenic 60.0%
GGGGTGGGTCGGACCTTAAA+AGG + chr8.2:2388869-2388888 MS.gene061218:CDS 60.0%
TCACGTCGTCACAACCACGG+CGG + chr8.2:2388602-2388621 MS.gene061218:CDS 60.0%
TGCGCGTGAATCCCAATCGC+CGG - chr8.2:2388579-2388598 None:intergenic 60.0%
TTTGGCGCGTGGCCGGAAAA+TGG + chr8.2:2388826-2388845 MS.gene061218:CDS 60.0%
! CGGTTTTCGAAGGGGTGGGT+CGG + chr8.2:2388858-2388877 MS.gene061218:CDS 60.0%
!! ATCAGCACCGGTGAACGTGC+CGG + chr8.2:2388712-2388731 MS.gene061218:CDS 60.0%
CACCGGTGAACGTGCCGGAT+TGG + chr8.2:2388717-2388736 MS.gene061218:CDS 65.0%
GCGCGTGAATCCCAATCGCC+GGG - chr8.2:2388578-2388597 None:intergenic 65.0%
GGCGCGTGGCCGGAAAATGG+CGG + chr8.2:2388829-2388848 MS.gene061218:CDS 70.0%
ACGCTTGCGACAGGCCGCCG+TGG - chr8.2:2388619-2388638 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 2388419 2388949 2388419 ID=MS.gene061218
chr8.2 mRNA 2388419 2388949 2388419 ID=MS.gene061218.t1;Parent=MS.gene061218
chr8.2 exon 2388419 2388949 2388419 ID=MS.gene061218.t1.exon1;Parent=MS.gene061218.t1
chr8.2 CDS 2388419 2388949 2388419 ID=cds.MS.gene061218.t1;Parent=MS.gene061218.t1
Gene Sequence

>MS.gene061218

ATGGCGAAGGGTCGCAAATTCACCACCAACCGAAACGAACGGTTGTTAGGAACAAACTATATTCAAAGCTCCACCACCACTCATGAAGTAACCGATTTCAGGGAAGAAGATGTGTGGTCCATGGTTGAAGATCGTGACCACAACGTCAATTTCTCTCCCGGCGATTGGGATTCACGCGCACATTCACGTCGTCACAACCACGGCGGCCTGTCGCAAGCGTTTGAGAATCAGGTTAGTGATGTTGCGACTATGTCGTCGAGAATCGTTCACCACCAGTACCGGAACGTTGCAACATCAGCACCGGTGAACGTGCCGGATTGGAGTAAGATACTCCGAGTTGAATCGGTGGAATCGTTGCATGATATGGATGATGATAACGAATCGGAGATGGTTCCGCCGCATGAGTATTTGGCGCGTGGCCGGAAAATGGCGGCAAATTCGGTTTTCGAAGGGGTGGGTCGGACCTTAAAAGGCCGTGATTTGAGACGGGTTCGTGATGCTGTTTGGAACCAAACCGGGTTCGATGGTTGA

Protein sequence

>MS.gene061218.t1

MAKGRKFTTNRNERLLGTNYIQSSTTTHEVTDFREEDVWSMVEDRDHNVNFSPGDWDSRAHSRRHNHGGLSQAFENQVSDVATMSSRIVHHQYRNVATSAPVNVPDWSKILRVESVESLHDMDDDNESEMVPPHEYLARGRKMAANSVFEGVGRTLKGRDLRRVRDAVWNQTGFDG