Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62457.t1 | XP_003609259.1 | 95.3 | 85 | 4 | 0 | 1 | 85 | 1 | 85 | 1.40E-40 | 175.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62457.t1 | G7JVA0 | 95.3 | 85 | 4 | 0 | 1 | 85 | 1 | 85 | 1.0e-40 | 175.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050442 | MS.gene62457 | 0.801504 | 8.73E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene62457 | 0.805908 | 1.06E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene62457 | 0.806141 | 9.50E-50 | -1.69E-46 |
| MS.gene054542 | MS.gene62457 | 0.803771 | 2.97E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene62457 | 0.807991 | 3.86E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62457.t1 | MTR_4g113750 | 95.294 | 85 | 4 | 0 | 1 | 85 | 1 | 85 | 3.90e-56 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62457.t1 | AT1G75125 | 54.795 | 73 | 33 | 0 | 13 | 85 | 11 | 83 | 2.55e-25 | 91.3 |
Find 24 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGGAAATCGCCTTGGAAT+TGG | 0.320897 | 8.2:+14511568 | None:intergenic |
| ACTTTGTGTCCGAGAATAAA+CGG | 0.396004 | 8.2:-14511465 | MS.gene62457:CDS |
| AATCTTTACCCGTTTATTCT+CGG | 0.396090 | 8.2:+14511456 | None:intergenic |
| CGATTTCCAACATGGATTAC+CGG | 0.398072 | 8.2:-14511556 | MS.gene62457:CDS |
| ACAACGTTGTCTGAATTGTC+TGG | 0.418889 | 8.2:-14511085 | MS.gene62457:CDS |
| ACACATGGGTTAATTGCAAC+AGG | 0.458488 | 8.2:+14511251 | None:intergenic |
| CTTTGTGTCCGAGAATAAAC+GGG | 0.475435 | 8.2:-14511464 | MS.gene62457:intron |
| TGTCTGAATTGTCTGGGAAA+AGG | 0.496121 | 8.2:-14511078 | MS.gene62457:CDS |
| TTCCAAGGCGATTTCCAACA+TGG | 0.513408 | 8.2:-14511564 | MS.gene62457:CDS |
| ATCCATGTTGGAAATCGCCT+TGG | 0.531843 | 8.2:+14511562 | None:intergenic |
| ATATATGCAAGTTATGCAGC+GGG | 0.546291 | 8.2:-14511307 | MS.gene62457:CDS |
| TGTAAAAGTTGTAGAGGGAA+AGG | 0.556567 | 8.2:-14511335 | MS.gene62457:CDS |
| GTAAAAGTTGTAGAGGGAAA+GGG | 0.562827 | 8.2:-14511334 | MS.gene62457:CDS |
| CAACGTTGTCTGAATTGTCT+GGG | 0.565752 | 8.2:-14511084 | MS.gene62457:CDS |
| GCGGGAATTCAACCATAGCT+TGG | 0.577529 | 8.2:-14511289 | MS.gene62457:CDS |
| CCCCAGTATTACAAAAGCAC+CGG | 0.584681 | 8.2:+14511537 | None:intergenic |
| AAAGCACCGGTAATCCATGT+TGG | 0.590454 | 8.2:+14511550 | None:intergenic |
| TTGCCGTGTAAAAGTTGTAG+AGG | 0.598560 | 8.2:-14511341 | MS.gene62457:CDS |
| ATTGCAACAGGATCATACAT+TGG | 0.599710 | 8.2:+14511263 | None:intergenic |
| AAGAAATGAATCCAATTCCA+AGG | 0.602574 | 8.2:-14511579 | None:intergenic |
| ATACATTGGAGACCAAGCTA+TGG | 0.629079 | 8.2:+14511277 | None:intergenic |
| TATATATGCAAGTTATGCAG+CGG | 0.648101 | 8.2:-14511308 | MS.gene62457:CDS |
| TGCCGTGTAAAAGTTGTAGA+GGG | 0.654823 | 8.2:-14511340 | MS.gene62457:CDS |
| GGGAATAGCAACTCTGAACG+TGG | 0.673296 | 8.2:-14511515 | MS.gene62457:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATTATAGTAACTGATAA+TGG | - | chr8.2:14511524-14511543 | MS.gene62457:CDS | 20.0% |
| !! | TTTGCTTTCATTTGAATATT+GGG | - | chr8.2:14511472-14511491 | MS.gene62457:CDS | 20.0% |
| !!! | TTTTGCTTTCATTTGAATAT+TGG | - | chr8.2:14511471-14511490 | MS.gene62457:CDS | 20.0% |
| ! | TTGCTTTCATTTGAATATTG+GGG | - | chr8.2:14511473-14511492 | MS.gene62457:CDS | 25.0% |
| AATCTTTACCCGTTTATTCT+CGG | + | chr8.2:14511193-14511212 | None:intergenic | 30.0% | |
| GATAATATTGATGTGCTTTC+TGG | - | chr8.2:14511249-14511268 | MS.gene62457:intron | 30.0% | |
| TATATATGCAAGTTATGCAG+CGG | - | chr8.2:14511338-14511357 | MS.gene62457:CDS | 30.0% | |
| TGTCTTGAAATTGAATTGCA+AGG | - | chr8.2:14511502-14511521 | MS.gene62457:CDS | 30.0% | |
| ACTTTGTGTCCGAGAATAAA+CGG | - | chr8.2:14511181-14511200 | MS.gene62457:intron | 35.0% | |
| ATATATGCAAGTTATGCAGC+GGG | - | chr8.2:14511339-14511358 | MS.gene62457:CDS | 35.0% | |
| ATTGCAACAGGATCATACAT+TGG | + | chr8.2:14511386-14511405 | None:intergenic | 35.0% | |
| GTAAAAGTTGTAGAGGGAAA+GGG | - | chr8.2:14511312-14511331 | MS.gene62457:CDS | 35.0% | |
| TGTAAAAGTTGTAGAGGGAA+AGG | - | chr8.2:14511311-14511330 | MS.gene62457:CDS | 35.0% | |
| ! | TTCCCTCTACAACTTTTACA+CGG | + | chr8.2:14511311-14511330 | None:intergenic | 35.0% |
| ! | TTGATGTGCTTTCTGGAAAT+TGG | - | chr8.2:14511256-14511275 | MS.gene62457:CDS | 35.0% |
| !!! | ACTGATAATGGTTTTGTTGC+AGG | - | chr8.2:14511536-14511555 | MS.gene62457:CDS | 35.0% |
| !!! | CTGATAATGGTTTTGTTGCA+GGG | - | chr8.2:14511537-14511556 | MS.gene62457:CDS | 35.0% |
| ACAACGTTGTCTGAATTGTC+TGG | - | chr8.2:14511561-14511580 | MS.gene62457:CDS | 40.0% | |
| ATACATTGGAGACCAAGCTA+TGG | + | chr8.2:14511372-14511391 | None:intergenic | 40.0% | |
| CAACGTTGTCTGAATTGTCT+GGG | - | chr8.2:14511562-14511581 | MS.gene62457:CDS | 40.0% | |
| CTTTGTGTCCGAGAATAAAC+GGG | - | chr8.2:14511182-14511201 | MS.gene62457:intron | 40.0% | |
| TGCCGTGTAAAAGTTGTAGA+GGG | - | chr8.2:14511306-14511325 | MS.gene62457:CDS | 40.0% | |
| TGTCTGAATTGTCTGGGAAA+AGG | - | chr8.2:14511568-14511587 | MS.gene62457:CDS | 40.0% | |
| TTGCCGTGTAAAAGTTGTAG+AGG | - | chr8.2:14511305-14511324 | MS.gene62457:CDS | 40.0% | |
| ! | ACACATGGGTTAATTGCAAC+AGG | + | chr8.2:14511398-14511417 | None:intergenic | 40.0% |
| ! | ACCGGTGCTTTTGTAATACT+GGG | - | chr8.2:14511108-14511127 | MS.gene62457:intron | 40.0% |
| ! | CGATTTCCAACATGGATTAC+CGG | - | chr8.2:14511090-14511109 | MS.gene62457:CDS | 40.0% |
| ! | TACCGGTGCTTTTGTAATAC+TGG | - | chr8.2:14511107-14511126 | MS.gene62457:intron | 40.0% |
| AACCTGTTTCCATCGCAAGT+AGG | + | chr8.2:14511425-14511444 | None:intergenic | 45.0% | |
| ATCCATGTTGGAAATCGCCT+TGG | + | chr8.2:14511087-14511106 | None:intergenic | 45.0% | |
| CGCAAGTAGGACAAACACAT+GGG | + | chr8.2:14511412-14511431 | None:intergenic | 45.0% | |
| GTTGGAAATCGCCTTGGAAT+TGG | + | chr8.2:14511081-14511100 | None:intergenic | 45.0% | |
| TCGCAAGTAGGACAAACACA+TGG | + | chr8.2:14511413-14511432 | None:intergenic | 45.0% | |
| TGTGTTTGTCCTACTTGCGA+TGG | - | chr8.2:14511413-14511432 | MS.gene62457:intron | 45.0% | |
| TTCCAAGGCGATTTCCAACA+TGG | - | chr8.2:14511082-14511101 | MS.gene62457:CDS | 45.0% | |
| ! | CCCCAGTATTACAAAAGCAC+CGG | + | chr8.2:14511112-14511131 | None:intergenic | 45.0% |
| ! | CCGGTGCTTTTGTAATACTG+GGG | - | chr8.2:14511109-14511128 | MS.gene62457:intron | 45.0% |
| ! | CGGTGCTTTTGTAATACTGG+GGG | - | chr8.2:14511110-14511129 | MS.gene62457:intron | 45.0% |
| ! | GGTGCTTTTGTAATACTGGG+GGG | - | chr8.2:14511111-14511130 | MS.gene62457:intron | 45.0% |
| !! | AAAGCACCGGTAATCCATGT+TGG | + | chr8.2:14511099-14511118 | None:intergenic | 45.0% |
| GCGGGAATTCAACCATAGCT+TGG | - | chr8.2:14511357-14511376 | MS.gene62457:intron | 50.0% | |
| GGGAATAGCAACTCTGAACG+TGG | - | chr8.2:14511131-14511150 | MS.gene62457:intron | 50.0% | |
| GTCCTACTTGCGATGGAAAC+AGG | - | chr8.2:14511420-14511439 | MS.gene62457:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 14511072 | 14511596 | 14511072 | ID=MS.gene62457 |
| chr8.2 | mRNA | 14511072 | 14511596 | 14511072 | ID=MS.gene62457.t1;Parent=MS.gene62457 |
| chr8.2 | exon | 14511465 | 14511596 | 14511465 | ID=MS.gene62457.t1.exon1;Parent=MS.gene62457.t1 |
| chr8.2 | CDS | 14511465 | 14511596 | 14511465 | ID=cds.MS.gene62457.t1;Parent=MS.gene62457.t1 |
| chr8.2 | exon | 14511253 | 14511375 | 14511253 | ID=MS.gene62457.t1.exon2;Parent=MS.gene62457.t1 |
| chr8.2 | CDS | 14511253 | 14511375 | 14511253 | ID=cds.MS.gene62457.t1;Parent=MS.gene62457.t1 |
| chr8.2 | exon | 14511072 | 14511110 | 14511072 | ID=MS.gene62457.t1.exon3;Parent=MS.gene62457.t1 |
| chr8.2 | CDS | 14511072 | 14511110 | 14511072 | ID=cds.MS.gene62457.t1;Parent=MS.gene62457.t1 |
| Gene Sequence |
| Protein sequence |
