Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63411.t1 | XP_013445629.1 | 96.4 | 194 | 4 | 1 | 1 | 194 | 1 | 191 | 4.80E-104 | 387.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63411.t1 | Q6R567 | 46.1 | 204 | 91 | 7 | 1 | 194 | 5 | 199 | 2.5e-40 | 166.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63411.t1 | A0A072U1G6 | 96.4 | 194 | 4 | 1 | 1 | 194 | 1 | 191 | 3.5e-104 | 387.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene63411 | MS.gene34211 | PPI |
| MS.gene63411 | MS.gene032302 | PPI |
| MS.gene36072 | MS.gene63411 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63411.t1 | MTR_8g465910 | 95.897 | 195 | 5 | 1 | 1 | 195 | 1 | 192 | 3.20e-139 | 388 |
| MS.gene63411.t1 | MTR_2g090295 | 52.913 | 206 | 75 | 7 | 1 | 195 | 1 | 195 | 3.29e-69 | 211 |
| MS.gene63411.t1 | MTR_2g090330 | 60.909 | 110 | 39 | 1 | 36 | 145 | 25 | 130 | 7.56e-46 | 149 |
| MS.gene63411.t1 | MTR_2g090330 | 60.909 | 110 | 39 | 1 | 36 | 145 | 22 | 127 | 1.13e-45 | 148 |
| MS.gene63411.t1 | MTR_2g090325 | 52.055 | 146 | 43 | 5 | 36 | 168 | 10 | 141 | 2.38e-41 | 139 |
| MS.gene63411.t1 | MTR_4g088875 | 44.295 | 149 | 74 | 4 | 24 | 165 | 11 | 157 | 1.44e-32 | 117 |
| MS.gene63411.t1 | MTR_2g013550 | 42.690 | 171 | 79 | 6 | 36 | 190 | 19 | 186 | 7.93e-32 | 115 |
| MS.gene63411.t1 | MTR_4g088870 | 46.429 | 112 | 54 | 3 | 24 | 131 | 11 | 120 | 2.20e-31 | 113 |
| MS.gene63411.t1 | MTR_1g021895 | 48.421 | 95 | 40 | 2 | 41 | 135 | 27 | 112 | 5.58e-28 | 105 |
| MS.gene63411.t1 | MTR_5g019300 | 48.913 | 92 | 38 | 2 | 44 | 135 | 35 | 117 | 1.59e-27 | 104 |
| MS.gene63411.t1 | MTR_1g054260 | 38.136 | 118 | 69 | 3 | 22 | 135 | 62 | 179 | 2.63e-23 | 92.4 |
| MS.gene63411.t1 | MTR_1g054465 | 38.542 | 96 | 58 | 1 | 39 | 133 | 50 | 145 | 4.52e-23 | 91.7 |
| MS.gene63411.t1 | MTR_4g029440 | 45.652 | 92 | 42 | 2 | 44 | 135 | 126 | 209 | 7.95e-23 | 94.7 |
| MS.gene63411.t1 | MTR_4g029440 | 45.652 | 92 | 42 | 2 | 44 | 135 | 132 | 215 | 8.60e-23 | 94.7 |
| MS.gene63411.t1 | MTR_1g054210 | 44.565 | 92 | 43 | 3 | 44 | 135 | 122 | 205 | 1.76e-22 | 90.9 |
| MS.gene63411.t1 | MTR_1g054280 | 40.000 | 110 | 52 | 3 | 42 | 151 | 74 | 169 | 2.36e-22 | 91.3 |
| MS.gene63411.t1 | MTR_7g093390 | 43.478 | 92 | 41 | 2 | 44 | 135 | 170 | 250 | 3.25e-22 | 93.2 |
| MS.gene63411.t1 | MTR_2g061230 | 38.889 | 108 | 55 | 3 | 28 | 135 | 94 | 190 | 5.59e-20 | 84.3 |
| MS.gene63411.t1 | MTR_1g015390 | 37.333 | 75 | 35 | 2 | 34 | 107 | 353 | 416 | 3.61e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63411.t1 | AT4G03510 | 41.872 | 203 | 104 | 6 | 1 | 195 | 1 | 197 | 3.42e-40 | 137 |
| MS.gene63411.t1 | AT4G03510 | 41.872 | 203 | 104 | 6 | 1 | 195 | 1 | 197 | 3.42e-40 | 137 |
| MS.gene63411.t1 | AT4G03510 | 41.872 | 203 | 104 | 6 | 1 | 195 | 1 | 197 | 3.42e-40 | 137 |
| MS.gene63411.t1 | AT4G03510 | 41.872 | 203 | 104 | 6 | 1 | 195 | 1 | 197 | 3.42e-40 | 137 |
| MS.gene63411.t1 | AT4G27470 | 41.176 | 170 | 84 | 5 | 39 | 195 | 37 | 203 | 1.45e-34 | 122 |
| MS.gene63411.t1 | AT4G28270 | 39.506 | 162 | 66 | 5 | 39 | 195 | 14 | 148 | 1.79e-31 | 113 |
| MS.gene63411.t1 | AT1G74990 | 37.903 | 124 | 64 | 3 | 29 | 152 | 2 | 112 | 5.32e-28 | 102 |
| MS.gene63411.t1 | AT1G19310 | 51.087 | 92 | 36 | 2 | 44 | 135 | 21 | 103 | 6.94e-28 | 105 |
| MS.gene63411.t1 | AT2G23780 | 45.098 | 102 | 46 | 3 | 35 | 135 | 16 | 108 | 4.74e-26 | 100 |
| MS.gene63411.t1 | AT2G23780 | 45.098 | 102 | 46 | 3 | 35 | 135 | 16 | 108 | 4.74e-26 | 100 |
| MS.gene63411.t1 | AT2G23780 | 45.098 | 102 | 46 | 3 | 35 | 135 | 16 | 108 | 4.74e-26 | 100 |
| MS.gene63411.t1 | AT3G58030 | 41.667 | 108 | 48 | 3 | 31 | 135 | 121 | 216 | 1.86e-22 | 94.0 |
| MS.gene63411.t1 | AT3G58030 | 41.667 | 108 | 48 | 3 | 31 | 135 | 121 | 216 | 1.86e-22 | 94.0 |
| MS.gene63411.t1 | AT3G58030 | 41.667 | 108 | 48 | 3 | 31 | 135 | 121 | 216 | 1.86e-22 | 94.0 |
| MS.gene63411.t1 | AT3G58030 | 41.667 | 108 | 48 | 3 | 31 | 135 | 121 | 216 | 1.86e-22 | 94.0 |
| MS.gene63411.t1 | AT2G42030 | 41.414 | 99 | 44 | 3 | 44 | 141 | 139 | 224 | 9.91e-22 | 91.7 |
| MS.gene63411.t1 | AT2G44410 | 31.884 | 138 | 80 | 3 | 4 | 135 | 77 | 206 | 7.10e-19 | 83.6 |
| MS.gene63411.t1 | AT2G26350 | 40.964 | 83 | 32 | 4 | 25 | 107 | 312 | 377 | 6.11e-11 | 60.8 |
| MS.gene63411.t1 | AT2G26350 | 40.964 | 83 | 32 | 4 | 25 | 107 | 241 | 306 | 7.43e-11 | 60.5 |
Find 49 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCGCTAGTTCCATTATA+TGG | 0.310263 | 4.4:-60076383 | MS.gene63411:CDS |
| ATCTTTGGTGAAATGATATA+TGG | 0.327321 | 4.4:-60076134 | MS.gene63411:CDS |
| GTTGTAGCATTAAATGATCT+TGG | 0.349454 | 4.4:+60076284 | None:intergenic |
| GTTTGGCCACGGCCATATAA+TGG | 0.354971 | 4.4:+60076371 | None:intergenic |
| GATAACACATACGGAATCTT+TGG | 0.359665 | 4.4:-60076149 | MS.gene63411:CDS |
| CTTCAGACACAGTCATGGTT+TGG | 0.381200 | 4.4:+60076354 | None:intergenic |
| ATGATATATGGAAGGATGTT+TGG | 0.416293 | 4.4:-60076122 | MS.gene63411:intron |
| TGTTGATAACTTTGATAAGT+AGG | 0.417086 | 4.4:+60076251 | None:intergenic |
| TTTGTGGTCATCTTTACTGC+TGG | 0.419101 | 4.4:-60076505 | MS.gene63411:CDS |
| GCTGGCCTTGCATTTACAAA+TGG | 0.444667 | 4.4:-60076487 | MS.gene63411:CDS |
| GAAGCAGAAGCCACAATGTC+CGG | 0.455529 | 4.4:-60076429 | MS.gene63411:CDS |
| ATCGTCCATGTACTGATCTA+AGG | 0.463858 | 4.4:+60076679 | None:intergenic |
| CAAAGATGGCTTATCTTCAA+AGG | 0.467141 | 4.4:+60076637 | None:intergenic |
| CTTTGACTGTAACATCTGCC+TGG | 0.478100 | 4.4:-60076555 | MS.gene63411:CDS |
| CTTGGACCCGTAGGTCTTCG+AGG | 0.488512 | 4.4:+60076302 | None:intergenic |
| GAGAGTTCTGATTTGCATAC+CGG | 0.493695 | 4.4:+60076410 | None:intergenic |
| AAGGCAAGGCTCAGCAAGTA+GGG | 0.503764 | 4.4:-60076334 | MS.gene63411:CDS |
| TGTCGTGCCTCGAAGACCTA+CGG | 0.507710 | 4.4:-60076309 | MS.gene63411:CDS |
| CCGGGTCTTGCACACAATCC+AGG | 0.516479 | 4.4:+60076537 | None:intergenic |
| GTCGTGCCTCGAAGACCTAC+GGG | 0.518622 | 4.4:-60076308 | MS.gene63411:CDS |
| GATGTTTAGTACAAGTGGTT+CGG | 0.525093 | 4.4:-60076177 | MS.gene63411:CDS |
| GAAGGCAAGGCTCAGCAAGT+AGG | 0.536195 | 4.4:-60076335 | MS.gene63411:CDS |
| ATCTGCAATCACATCGGTGG+AGG | 0.538872 | 4.4:+60076601 | None:intergenic |
| TCACCGATGTTTAGTACAAG+TGG | 0.543488 | 4.4:-60076182 | MS.gene63411:CDS |
| CATGACTGTGTCTGAAGGCA+AGG | 0.550095 | 4.4:-60076348 | MS.gene63411:CDS |
| ATCAACATCCTCATCATCCT+CGG | 0.557432 | 4.4:-60076235 | MS.gene63411:CDS |
| ATCGGTGAAGGGTAACTGCT+CGG | 0.568341 | 4.4:+60076197 | None:intergenic |
| CTTGCCTTCAGACACAGTCA+TGG | 0.574821 | 4.4:+60076349 | None:intergenic |
| CCTGGATTGTGTGCAAGACC+CGG | 0.575606 | 4.4:-60076537 | MS.gene63411:CDS |
| AAAGGTATCAATTTGAGAGA+CGG | 0.577335 | 4.4:+60076655 | None:intergenic |
| CTAGTTCCATTATATGGCCG+TGG | 0.589093 | 4.4:-60076377 | MS.gene63411:CDS |
| CTTGTACTAAACATCGGTGA+AGG | 0.589508 | 4.4:+60076185 | None:intergenic |
| TCAAGCCATTTGTAAATGCA+AGG | 0.597318 | 4.4:+60076482 | None:intergenic |
| GATTTGCATACCGGACATTG+TGG | 0.600937 | 4.4:+60076419 | None:intergenic |
| GGAATTGAGTTGAATTGCCG+AGG | 0.606224 | 4.4:+60076218 | None:intergenic |
| TTAAATGATCTTGGACCCGT+AGG | 0.606770 | 4.4:+60076293 | None:intergenic |
| TTGTACTAAACATCGGTGAA+GGG | 0.617159 | 4.4:+60076186 | None:intergenic |
| TCTGATAGAAATGCATCTAG+TGG | 0.620682 | 4.4:-60076578 | MS.gene63411:CDS |
| ACACAGTCATGGTTTGGCCA+CGG | 0.630922 | 4.4:+60076360 | None:intergenic |
| TTGAATTGCCGAGGATGATG+AGG | 0.635828 | 4.4:+60076227 | None:intergenic |
| AGAATCTGCAATCACATCGG+TGG | 0.639252 | 4.4:+60076598 | None:intergenic |
| AAATGGCTTGACATACAAAG+TGG | 0.643704 | 4.4:-60076470 | MS.gene63411:CDS |
| TTGGTGAAATGATATATGGA+AGG | 0.648778 | 4.4:-60076130 | MS.gene63411:CDS |
| TCGGCACTAGATAACACATA+CGG | 0.649004 | 4.4:-60076158 | MS.gene63411:CDS |
| CAAACCATGACTGTGTCTGA+AGG | 0.652258 | 4.4:-60076353 | MS.gene63411:CDS |
| GCCATATAATGGAACTAGCG+AGG | 0.658937 | 4.4:+60076382 | None:intergenic |
| AATGGCCTTAGATCAGTACA+TGG | 0.660626 | 4.4:-60076684 | MS.gene63411:CDS |
| ATCAGAATCTGCAATCACAT+CGG | 0.660904 | 4.4:+60076595 | None:intergenic |
| GAACCACTTGTACTAAACAT+CGG | 0.669442 | 4.4:+60076179 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTAATTCTTATAGTTTTCAA+GGG | - | chr4.4:60076598-60076617 | MS.gene63411:CDS | 20.0% |
| ! | ATCTTTGGTGAAATGATATA+TGG | - | chr4.4:60076534-60076553 | MS.gene63411:CDS | 25.0% |
| ! | CCTTGAAAACTATAAGAATT+AGG | + | chr4.4:60076600-60076619 | None:intergenic | 25.0% |
| !! | ATGTATACATGTTTTCTATC+TGG | + | chr4.4:60076575-60076594 | None:intergenic | 25.0% |
| !! | CCTAATTCTTATAGTTTTCA+AGG | - | chr4.4:60076597-60076616 | MS.gene63411:CDS | 25.0% |
| !!! | TGTTGATAACTTTGATAAGT+AGG | + | chr4.4:60076420-60076439 | None:intergenic | 25.0% |
| ATGATATATGGAAGGATGTT+TGG | - | chr4.4:60076546-60076565 | MS.gene63411:CDS | 30.0% | |
| ATTAAATGTCTTCTCATCCT+TGG | + | chr4.4:60076633-60076652 | None:intergenic | 30.0% | |
| GTTGTAGCATTAAATGATCT+TGG | + | chr4.4:60076387-60076406 | None:intergenic | 30.0% | |
| TTGGTGAAATGATATATGGA+AGG | - | chr4.4:60076538-60076557 | MS.gene63411:CDS | 30.0% | |
| ! | ATAAGCCATCTTTGAAAAAC+TGG | - | chr4.4:60076040-60076059 | MS.gene63411:intron | 30.0% |
| !! | AAAGGTATCAATTTGAGAGA+CGG | + | chr4.4:60076016-60076035 | None:intergenic | 30.0% |
| !! | GTTTTCCAGTTTTTCAAAGA+TGG | + | chr4.4:60076048-60076067 | None:intergenic | 30.0% |
| AAATGGCTTGACATACAAAG+TGG | - | chr4.4:60076198-60076217 | MS.gene63411:CDS | 35.0% | |
| ATCAGAATCTGCAATCACAT+CGG | + | chr4.4:60076076-60076095 | None:intergenic | 35.0% | |
| CAAAGATGGCTTATCTTCAA+AGG | + | chr4.4:60076034-60076053 | None:intergenic | 35.0% | |
| GAACCACTTGTACTAAACAT+CGG | + | chr4.4:60076492-60076511 | None:intergenic | 35.0% | |
| GATAACACATACGGAATCTT+TGG | - | chr4.4:60076519-60076538 | MS.gene63411:CDS | 35.0% | |
| GATGTTTAGTACAAGTGGTT+CGG | - | chr4.4:60076491-60076510 | MS.gene63411:CDS | 35.0% | |
| TCAAGCCATTTGTAAATGCA+AGG | + | chr4.4:60076189-60076208 | None:intergenic | 35.0% | |
| TCTGATAGAAATGCATCTAG+TGG | - | chr4.4:60076090-60076109 | MS.gene63411:intron | 35.0% | |
| TCTTCTCATCCTTGGATTAT+TGG | + | chr4.4:60076625-60076644 | None:intergenic | 35.0% | |
| TTGTACTAAACATCGGTGAA+GGG | + | chr4.4:60076485-60076504 | None:intergenic | 35.0% | |
| ATCAACATCCTCATCATCCT+CGG | - | chr4.4:60076433-60076452 | MS.gene63411:CDS | 40.0% | |
| CTTGTACTAAACATCGGTGA+AGG | + | chr4.4:60076486-60076505 | None:intergenic | 40.0% | |
| TCACCGATGTTTAGTACAAG+TGG | - | chr4.4:60076486-60076505 | MS.gene63411:CDS | 40.0% | |
| TCCTCGCTAGTTCCATTATA+TGG | - | chr4.4:60076285-60076304 | MS.gene63411:CDS | 40.0% | |
| TCGGCACTAGATAACACATA+CGG | - | chr4.4:60076510-60076529 | MS.gene63411:CDS | 40.0% | |
| TGACCACAAAAAGTGACAAC+CGG | + | chr4.4:60076153-60076172 | None:intergenic | 40.0% | |
| TTAAATGATCTTGGACCCGT+AGG | + | chr4.4:60076378-60076397 | None:intergenic | 40.0% | |
| TTCAAGGGACCAATAATCCA+AGG | - | chr4.4:60076613-60076632 | MS.gene63411:CDS | 40.0% | |
| TTTGTGGTCATCTTTACTGC+TGG | - | chr4.4:60076163-60076182 | MS.gene63411:CDS | 40.0% | |
| ! | ATCGTCCATGTACTGATCTA+AGG | + | chr4.4:60075992-60076011 | None:intergenic | 40.0% |
| ! | GAGAGTTCTGATTTGCATAC+CGG | + | chr4.4:60076261-60076280 | None:intergenic | 40.0% |
| AGAATCTGCAATCACATCGG+TGG | + | chr4.4:60076073-60076092 | None:intergenic | 45.0% | |
| CAAACCATGACTGTGTCTGA+AGG | - | chr4.4:60076315-60076334 | MS.gene63411:CDS | 45.0% | |
| CTAGTTCCATTATATGGCCG+TGG | - | chr4.4:60076291-60076310 | MS.gene63411:CDS | 45.0% | |
| CTTCAGACACAGTCATGGTT+TGG | + | chr4.4:60076317-60076336 | None:intergenic | 45.0% | |
| CTTTGACTGTAACATCTGCC+TGG | - | chr4.4:60076113-60076132 | MS.gene63411:intron | 45.0% | |
| GACCACAAAAAGTGACAACC+GGG | + | chr4.4:60076152-60076171 | None:intergenic | 45.0% | |
| GATTTGCATACCGGACATTG+TGG | + | chr4.4:60076252-60076271 | None:intergenic | 45.0% | |
| TTGAATTGCCGAGGATGATG+AGG | + | chr4.4:60076444-60076463 | None:intergenic | 45.0% | |
| ! | GCCATATAATGGAACTAGCG+AGG | + | chr4.4:60076289-60076308 | None:intergenic | 45.0% |
| ! | TGGCTTCTGCTTCTCGTTTT+CGG | + | chr4.4:60076232-60076251 | None:intergenic | 45.0% |
| !! | GCTGGCCTTGCATTTACAAA+TGG | - | chr4.4:60076181-60076200 | MS.gene63411:CDS | 45.0% |
| !! | GGAATTGAGTTGAATTGCCG+AGG | + | chr4.4:60076453-60076472 | None:intergenic | 45.0% |
| AAGGCAAGGCTCAGCAAGTA+GGG | - | chr4.4:60076334-60076353 | MS.gene63411:CDS | 50.0% | |
| ATCGGTGAAGGGTAACTGCT+CGG | + | chr4.4:60076474-60076493 | None:intergenic | 50.0% | |
| ATCTGCAATCACATCGGTGG+AGG | + | chr4.4:60076070-60076089 | None:intergenic | 50.0% | |
| CATGACTGTGTCTGAAGGCA+AGG | - | chr4.4:60076320-60076339 | MS.gene63411:CDS | 50.0% | |
| CTTGCCTTCAGACACAGTCA+TGG | + | chr4.4:60076322-60076341 | None:intergenic | 50.0% | |
| GAAGCAGAAGCCACAATGTC+CGG | - | chr4.4:60076239-60076258 | MS.gene63411:CDS | 50.0% | |
| GTTTGGCCACGGCCATATAA+TGG | + | chr4.4:60076300-60076319 | None:intergenic | 50.0% | |
| ! | ACACAGTCATGGTTTGGCCA+CGG | + | chr4.4:60076311-60076330 | None:intergenic | 50.0% |
| !!! | GACCCGGTTGTCACTTTTTG+TGG | - | chr4.4:60076147-60076166 | MS.gene63411:CDS | 50.0% |
| CCTGGATTGTGTGCAAGACC+CGG | - | chr4.4:60076131-60076150 | MS.gene63411:CDS | 55.0% | |
| GAAGGCAAGGCTCAGCAAGT+AGG | - | chr4.4:60076333-60076352 | MS.gene63411:CDS | 55.0% | |
| TGTCGTGCCTCGAAGACCTA+CGG | - | chr4.4:60076359-60076378 | MS.gene63411:CDS | 55.0% | |
| CCGGGTCTTGCACACAATCC+AGG | + | chr4.4:60076134-60076153 | None:intergenic | 60.0% | |
| CTTGGACCCGTAGGTCTTCG+AGG | + | chr4.4:60076369-60076388 | None:intergenic | 60.0% | |
| GTCGTGCCTCGAAGACCTAC+GGG | - | chr4.4:60076360-60076379 | MS.gene63411:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 60075985 | 60076705 | 60075985 | ID=MS.gene63411 |
| chr4.4 | mRNA | 60075985 | 60076705 | 60075985 | ID=MS.gene63411.t1;Parent=MS.gene63411 |
| chr4.4 | exon | 60076123 | 60076705 | 60076123 | ID=MS.gene63411.t1.exon1;Parent=MS.gene63411.t1 |
| chr4.4 | CDS | 60076123 | 60076705 | 60076123 | ID=cds.MS.gene63411.t1;Parent=MS.gene63411.t1 |
| chr4.4 | exon | 60075985 | 60075989 | 60075985 | ID=MS.gene63411.t1.exon2;Parent=MS.gene63411.t1 |
| chr4.4 | CDS | 60075985 | 60075989 | 60075985 | ID=cds.MS.gene63411.t1;Parent=MS.gene63411.t1 |
| Gene Sequence |
| Protein sequence |
