AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63735

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63735.t1 MTR_2g076210 94.005 367 22 0 1 367 1 367 0.0 727
MS.gene63735.t1 MTR_2g076200 74.731 372 85 5 1 367 4 371 0.0 564
MS.gene63735.t1 MTR_2g020280 47.340 376 158 9 21 367 29 393 1.65e-106 318
MS.gene63735.t1 MTR_5g020580 47.268 366 154 10 37 367 55 416 2.22e-102 308
MS.gene63735.t1 MTR_8g077195 46.277 376 165 7 25 367 41 412 1.84e-100 303
MS.gene63735.t1 MTR_1g088960 46.389 360 157 8 37 367 50 402 3.17e-97 295
MS.gene63735.t1 MTR_1g098790 43.928 387 153 9 37 367 53 431 7.25e-96 292
MS.gene63735.t1 MTR_1g022370 47.472 356 159 12 37 367 70 422 1.28e-94 289
MS.gene63735.t1 MTR_2g007930 43.536 379 161 10 37 367 54 427 3.52e-93 285
MS.gene63735.t1 MTR_2g020280 48.161 299 121 8 98 367 1 294 4.54e-80 247
MS.gene63735.t1 MTR_8g077620 42.416 356 176 9 25 360 40 386 2.43e-73 233
MS.gene63735.t1 MTR_8g077675 37.838 370 186 9 25 360 36 395 1.42e-66 216
MS.gene63735.t1 MTR_8g077640 37.830 341 142 11 36 357 94 383 2.29e-46 162
MS.gene63735.t1 MTR_8g077650 36.418 335 166 10 44 359 22 328 2.10e-44 156
MS.gene63735.t1 MTR_1g023800 50.000 98 49 0 35 132 50 147 1.71e-26 103
MS.gene63735.t1 MTR_0210s0010 37.209 86 48 1 80 165 9 88 7.57e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63735.t1 AT1G53320 61.979 384 124 7 1 367 1 379 2.07e-156 444
MS.gene63735.t1 AT2G47900 44.444 360 166 6 37 367 52 406 1.80e-101 306
MS.gene63735.t1 AT2G47900 44.444 360 166 6 37 367 52 406 1.80e-101 306
MS.gene63735.t1 AT2G47900 44.321 361 166 6 37 367 52 407 4.00e-101 305
MS.gene63735.t1 AT2G18280 46.705 349 165 5 37 367 48 393 7.22e-100 301
MS.gene63735.t1 AT2G18280 46.705 349 165 5 37 367 48 393 7.22e-100 301
MS.gene63735.t1 AT3G06380 45.028 362 166 9 31 367 27 380 2.98e-94 286
MS.gene63735.t1 AT1G25280 43.990 391 155 11 37 367 59 445 7.85e-94 287
MS.gene63735.t1 AT1G76900 40.594 404 166 9 35 367 55 455 1.49e-91 282
MS.gene63735.t1 AT1G76900 40.594 404 166 9 35 367 55 455 1.49e-91 282
MS.gene63735.t1 AT1G76900 40.594 404 166 9 35 367 55 455 1.49e-91 282
MS.gene63735.t1 AT1G47270 44.476 353 166 10 37 367 44 388 3.58e-88 271
MS.gene63735.t1 AT5G18680 43.432 373 170 10 25 367 28 389 4.53e-88 271
MS.gene63735.t1 AT1G47270 44.476 353 166 10 37 367 69 413 6.89e-88 271
MS.gene63735.t1 AT1G47270 44.476 353 166 10 37 367 68 412 7.96e-88 271
MS.gene63735.t1 AT1G43640 42.037 383 166 10 35 367 53 429 2.72e-87 270
MS.gene63735.t1 AT1G43640 42.276 369 157 10 49 367 1 363 1.12e-83 259
MS.gene63735.t1 AT3G06380 43.493 292 133 8 100 367 5 288 1.18e-67 215
MS.gene63735.t1 AT5G18680 42.809 299 132 8 98 367 9 297 5.82e-65 208
MS.gene63735.t1 AT1G25280 40.590 271 98 10 156 367 1 267 9.41e-50 168
MS.gene63735.t1 AT1G25280 40.590 271 98 10 156 367 1 267 9.41e-50 168
MS.gene63735.t1 AT1G61940 48.276 87 45 0 40 126 1 87 1.32e-22 96.3
MS.gene63735.t1 AT1G61940 49.180 61 31 0 40 100 1 61 1.20e-14 73.2

Find 92 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCACCACTGTGTTGTAAAT+TGG 0.184549 2.1:-23563210 MS.gene63735:CDS
ATCATTCGCCTTGGACGTTT+TGG 0.253223 2.1:+23562446 None:intergenic
ATGATATCCGCAAGAAGTTC+TGG 0.302123 2.1:+23564225 None:intergenic
GCCATTTGCTTAACTAGCTT+TGG 0.307225 2.1:-23562074 MS.gene63735:CDS
TGGGGAAGGTCGCGTGGCCC+TGG 0.326133 2.1:+23563229 None:intergenic
CGGCTTGTTCTGTCACTTGT+TGG 0.336212 2.1:+23562560 None:intergenic
TGTTCTGTCACTTGTTGGTT+TGG 0.343539 2.1:+23562565 None:intergenic
TTTCCCTTCTTGTCTTAAAC+AGG 0.347974 2.1:-23564070 MS.gene63735:intron
GATATCTTCTAGCTGCTAAT+AGG 0.354984 2.1:+23562960 None:intergenic
CTTGTTGGTTTGGCACCAGC+CGG 0.374243 2.1:+23562575 None:intergenic
TCTTCATCTCCTCTCCCTCC+TGG 0.374907 2.1:+23562182 None:intergenic
GCTATCTGAACTTTCGCATA+TGG 0.381471 2.1:+23562397 None:intergenic
ATCTTACTGACAAGCCAGTT+TGG 0.393584 2.1:+23562049 None:intergenic
TCGCCAGCATTAACAGATAC+AGG 0.396379 2.1:-23562989 MS.gene63735:intron
AACTAGCTTTGGCACCAAAC+TGG 0.411927 2.1:-23562063 MS.gene63735:CDS
GTACTTACTTACATGGTGTA+AGG 0.424832 2.1:+23563152 None:intergenic
CAAAGCCAACCAGGAGGGAG+AGG 0.425267 2.1:-23562191 MS.gene63735:CDS
ATATCACACGTGAGGTCGTC+GGG 0.441262 2.1:-23564119 MS.gene63735:CDS
CTGTCATATCCTTTCTCTCA+TGG 0.452722 2.1:+23562255 None:intergenic
GTCGGGTCACCTTCTCTTAA+CGG 0.458118 2.1:-23564102 MS.gene63735:CDS
CTTGAACTTCCATGAGAGAA+AGG 0.465557 2.1:-23562264 MS.gene63735:CDS
TCCGATGTGGAACGAGCAAT+TGG 0.466061 2.1:-23562297 MS.gene63735:CDS
TCCGTCCTCCGATGCACTAT+CGG 0.467168 2.1:+23564269 None:intergenic
CCTGCTGGTAACGTCGAGGC+AGG 0.472577 2.1:-23562503 MS.gene63735:CDS
CAAGGAAGTAATGCTTATGT+TGG 0.475540 2.1:-23562884 MS.gene63735:CDS
AAACGCATGGAAGGCAGATA+AGG 0.478629 2.1:+23562097 None:intergenic
CATCTCCTCTCCCTCCTGGT+TGG 0.480773 2.1:+23562186 None:intergenic
TTGTTGGTTTGGCACCAGCC+GGG 0.486862 2.1:+23562576 None:intergenic
TATTAGCAGCTAGAAGATAT+CGG 0.489255 2.1:-23562958 MS.gene63735:CDS
CTTGTGCCTGCGTTTGCAAA+CGG 0.489375 2.1:-23564149 MS.gene63735:CDS
ACAATTTATGATGCCCCGGC+TGG 0.489537 2.1:-23562590 MS.gene63735:CDS
CGTTACCAGCAGGAACTTGA+GGG 0.494917 2.1:+23562513 None:intergenic
AACGCATGGAAGGCAGATAA+GGG 0.500964 2.1:+23562098 None:intergenic
CCAATTTACAACACAGTGGT+GGG 0.506056 2.1:+23563210 None:intergenic
GCCAAAGCTAGTTAAGCAAA+TGG 0.506693 2.1:+23562073 None:intergenic
TGTTGGTTTGGCACCAGCCG+GGG 0.513154 2.1:+23562577 None:intergenic
AGAGAGCAAATCATTCGCCT+TGG 0.519881 2.1:+23562437 None:intergenic
CAGATACTTCAACAATGCTC+AGG 0.525873 2.1:-23564319 MS.gene63735:CDS
ATCAGTGAAGAACTTTCAGC+TGG 0.527624 2.1:-23562228 MS.gene63735:CDS
ATGCTTATGTTGGCAAGCTA+AGG 0.535171 2.1:-23562874 MS.gene63735:intron
TCCAATTTACAACACAGTGG+TGG 0.539488 2.1:+23563209 None:intergenic
ACGTTACCAGCAGGAACTTG+AGG 0.541472 2.1:+23562512 None:intergenic
AGGACCTGTTTAAGACAAGA+AGG 0.544045 2.1:+23564066 None:intergenic
CGCAAGAAGTTCTGGAAGCA+TGG 0.545732 2.1:+23564233 None:intergenic
CTTTCAGCTGGTTGCCACTG+TGG 0.546288 2.1:-23562216 MS.gene63735:CDS
ATAAGCCCTCAAGTTCCTGC+TGG 0.547760 2.1:-23562518 MS.gene63735:CDS
TGAACTTTCGCATATGGATG+AGG 0.550314 2.1:+23562403 None:intergenic
CATCGGAGGACGGAACAGAT+TGG 0.551175 2.1:-23564260 MS.gene63735:CDS
CTTCACAATTTATGATGCCC+CGG 0.551703 2.1:-23562594 MS.gene63735:CDS
CTTTCGCATATGGATGAGGC+AGG 0.555383 2.1:+23562407 None:intergenic
ACTATCGGTGGTGTCATCTC+CGG 0.555795 2.1:+23564284 None:intergenic
GGCAAGATAGAGGTAAAATG+TGG 0.562187 2.1:+23563174 None:intergenic
GATATCACACGTGAGGTCGT+CGG 0.562846 2.1:-23564120 MS.gene63735:CDS
ACTTCCTTGTGAAAAGTCAT+CGG 0.574248 2.1:+23562898 None:intergenic
TTTATTCTTCAAGATTGTGT+AGG 0.575970 2.1:+23562322 None:intergenic
GGAACGAGCAATTGGAGTGC+TGG 0.578985 2.1:-23562289 MS.gene63735:CDS
GCAAATGGCAAACGCATGGA+AGG 0.579450 2.1:+23562088 None:intergenic
CAGTGGTGGGGAAGGTCGCG+TGG 0.579862 2.1:+23563223 None:intergenic
ACCGATAGTGCATCGGAGGA+CGG 0.583942 2.1:-23564270 MS.gene63735:CDS
CCTGCCTCGACGTTACCAGC+AGG 0.584116 2.1:+23562503 None:intergenic
CGCCAGCATTAACAGATACA+GGG 0.585383 2.1:-23562988 MS.gene63735:intron
TACTTACTTACATGGTGTAA+GGG 0.588591 2.1:+23563153 None:intergenic
ACTGTGGATCAAAGCCAACC+AGG 0.595544 2.1:-23562200 MS.gene63735:CDS
TTAAGCAAATGGCAAACGCA+TGG 0.595995 2.1:+23562084 None:intergenic
GCTCAGGCTCTCAATGCTAC+CGG 0.598992 2.1:-23564303 MS.gene63735:CDS
TCTGTTTGCTTGCAAAACGC+CGG 0.603910 2.1:+23562540 None:intergenic
ATTAAACGAGTTGATGTCAC+GGG 0.605426 2.1:-23564204 MS.gene63735:CDS
TAAGGGCTGTCTATAGTACA+TGG 0.607232 2.1:+23562115 None:intergenic
GTGCCTGCGTTTGCAAACGG+TGG 0.610120 2.1:-23564146 MS.gene63735:CDS
TTACAACACAGTGGTGGGGA+AGG 0.615695 2.1:+23563215 None:intergenic
CATTAAACGAGTTGATGTCA+CGG 0.617052 2.1:-23564205 MS.gene63735:CDS
ATGGTGTAAGGGCAAGATAG+AGG 0.623484 2.1:+23563164 None:intergenic
CATGCTTCCAGAACTTCTTG+CGG 0.624235 2.1:-23564232 MS.gene63735:CDS
CAATTTACAACACAGTGGTG+GGG 0.625248 2.1:+23563211 None:intergenic
TCCAATTGCTCGTTCCACAT+CGG 0.629514 2.1:+23562296 None:intergenic
TGAAGAATAAAGCTCCGATG+TGG 0.636117 2.1:-23562310 MS.gene63735:CDS
TCTCCACCGTTTGCAAACGC+AGG 0.641600 2.1:+23564143 None:intergenic
TGACACCACCGATAGTGCAT+CGG 0.642812 2.1:-23564277 MS.gene63735:CDS
GGACCTGTTTAAGACAAGAA+GGG 0.643301 2.1:+23564067 None:intergenic
CACCACCGATAGTGCATCGG+AGG 0.644146 2.1:-23564274 MS.gene63735:CDS
CTGCTGGTAACGTCGAGGCA+GGG 0.646476 2.1:-23562502 MS.gene63735:CDS
TGGATCAAAGCCAACCAGGA+GGG 0.647654 2.1:-23562196 MS.gene63735:CDS
CAACAAGTGACAGAACAAGC+CGG 0.658598 2.1:-23562559 MS.gene63735:CDS
AAGATAGAGGTAAAATGTGG+AGG 0.660216 2.1:+23563177 None:intergenic
GTCCTCCGATGCACTATCGG+TGG 0.672145 2.1:+23564272 None:intergenic
TGGTTGGCTTTGATCCACAG+TGG 0.681134 2.1:+23562202 None:intergenic
GCGACGACATTTCGACGATG+CGG 0.691215 2.1:+23564171 None:intergenic
TTGATGTCACGGGAGAACAA+TGG 0.692340 2.1:-23564194 MS.gene63735:CDS
TATTCCAATTTACAACACAG+TGG 0.695058 2.1:+23563206 None:intergenic
GTGGAGAGATATCACACGTG+AGG 0.702332 2.1:-23564127 MS.gene63735:CDS
AGTTCCTGCTGGTAACGTCG+AGG 0.707570 2.1:-23562507 MS.gene63735:CDS
GTGGATCAAAGCCAACCAGG+AGG 0.710816 2.1:-23562197 MS.gene63735:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATATTATTTTAATATT+AGG - chr2.1:23563054-23563073 MS.gene63735:intron 0.0%
!! ATAATGATTAAAATTTCAAA+TGG - chr2.1:23563306-23563325 MS.gene63735:intron 10.0%
!! TTGAGAATAAATTCAATAAA+TGG + chr2.1:23563709-23563728 None:intergenic 15.0%
!! AAACCATTTATTGAAGAAAA+AGG - chr2.1:23563598-23563617 MS.gene63735:intron 20.0%
!! AACCATTTATTGAAGAAAAA+GGG - chr2.1:23563599-23563618 MS.gene63735:intron 20.0%
!! GATGCATTTATTAAGTATAT+AGG - chr2.1:23563029-23563048 MS.gene63735:intron 20.0%
!! TCAAACTATTAAAGAAGAAA+AGG + chr2.1:23562957-23562976 None:intergenic 20.0%
!! TTAAAATTTCAAATGGAGAT+TGG - chr2.1:23563313-23563332 MS.gene63735:intron 20.0%
!! TTTATGGATCATGAAAAATT+TGG - chr2.1:23562397-23562416 MS.gene63735:CDS 20.0%
!! TTTCAAATTATTAGTTGTGT+CGG + chr2.1:23562532-23562551 None:intergenic 20.0%
!! TTTCTCTCTTGAATAATTTA+TGG - chr2.1:23562381-23562400 MS.gene63735:CDS 20.0%
!!! ATCTTGTTATGTTTTGATAA+AGG - chr2.1:23562721-23562740 MS.gene63735:intron 20.0%
! ACAAACTAACTAATAACATC+TGG - chr2.1:23563671-23563690 MS.gene63735:intron 25.0%
! ATCAATTGAATTAAGAGAGT+TGG - chr2.1:23562689-23562708 MS.gene63735:intron 25.0%
! ATTTATTGAAGAAAAAGGGT+AGG - chr2.1:23563603-23563622 MS.gene63735:intron 25.0%
! TTTATTCTTCAAGATTGTGT+AGG + chr2.1:23564081-23564100 None:intergenic 25.0%
!! AAATGAATTCATTTTGTTGC+AGG + chr2.1:23562357-23562376 None:intergenic 25.0%
!! CTAACATTTTGTTCAATTAG+TGG - chr2.1:23562455-23562474 MS.gene63735:CDS 25.0%
!! TACCCTTTTTCTTCAATAAA+TGG + chr2.1:23563604-23563623 None:intergenic 25.0%
AACTAAAATGATCCTCATTC+AGG - chr2.1:23563569-23563588 MS.gene63735:intron 30.0%
CAAAACAGTACTTACTTACA+TGG + chr2.1:23563258-23563277 None:intergenic 30.0%
GAAAATGAAACCAAGTTACT+CGG - chr2.1:23562833-23562852 MS.gene63735:intron 30.0%
TACTTACTTACATGGTGTAA+GGG + chr2.1:23563250-23563269 None:intergenic 30.0%
TATTAGCAGCTAGAAGATAT+CGG - chr2.1:23563442-23563461 MS.gene63735:intron 30.0%
TATTCCAATTTACAACACAG+TGG + chr2.1:23563197-23563216 None:intergenic 30.0%
!! CATGTAAGTAAGTACTGTTT+TGG - chr2.1:23563256-23563275 MS.gene63735:intron 30.0%
!! GTTTTGGCAAAAGGATTATT+TGG - chr2.1:23563272-23563291 MS.gene63735:intron 30.0%
!! TTCTCCAGTTTTGTAAAGTA+GGG - chr2.1:23564249-23564268 MS.gene63735:CDS 30.0%
!!! CAAAAGGCTGAATTTTTTGT+AGG + chr2.1:23563931-23563950 None:intergenic 30.0%
AAAATCACGAGTTAACTCGT+AGG - chr2.1:23562749-23562768 MS.gene63735:intron 35.0%
AAGATAGAGGTAAAATGTGG+AGG + chr2.1:23563226-23563245 None:intergenic 35.0%
ACTCGTAGGATCATATGTTT+AGG - chr2.1:23562763-23562782 MS.gene63735:intron 35.0%
ACTTCCTTGTGAAAAGTCAT+CGG + chr2.1:23563505-23563524 None:intergenic 35.0%
CAAGGAAGTAATGCTTATGT+TGG - chr2.1:23563516-23563535 MS.gene63735:intron 35.0%
CTATCTATGAAGCATGGATA+CGG - chr2.1:23562478-23562497 MS.gene63735:CDS 35.0%
GTACTTACTTACATGGTGTA+AGG + chr2.1:23563251-23563270 None:intergenic 35.0%
TATTGAAGAAAAAGGGTAGG+AGG - chr2.1:23563606-23563625 MS.gene63735:intron 35.0%
TCTACGAGTTAATTTCACGT+AGG - chr2.1:23563091-23563110 MS.gene63735:intron 35.0%
TTAATATTCACCCCTTTGTC+CGG + chr2.1:23563647-23563666 None:intergenic 35.0%
TTTCCCTTCTTGTCTTAAAC+AGG - chr2.1:23562330-23562349 MS.gene63735:CDS 35.0%
! CAACAATCTTTTGCTTTGTC+TGG - chr2.1:23563726-23563745 MS.gene63735:intron 35.0%
! GATATCTTCTAGCTGCTAAT+AGG + chr2.1:23563443-23563462 None:intergenic 35.0%
! GTGATTTTTCCGTTAAGAGA+AGG + chr2.1:23562310-23562329 None:intergenic 35.0%
! TGGTTTTTCTTTCCTGAATG+AGG + chr2.1:23563584-23563603 None:intergenic 35.0%
!! ATTAAACGAGTTGATGTCAC+GGG - chr2.1:23562196-23562215 MS.gene63735:CDS 35.0%
!! CATTAAACGAGTTGATGTCA+CGG - chr2.1:23562195-23562214 MS.gene63735:CDS 35.0%
!! CTTCTCCAGTTTTGTAAAGT+AGG - chr2.1:23564248-23564267 MS.gene63735:CDS 35.0%
!! TCTCCAGTTTTGTAAAGTAG+GGG - chr2.1:23564250-23564269 MS.gene63735:CDS 35.0%
!!! GTAAGTACTGTTTTGGCAAA+AGG - chr2.1:23563263-23563282 MS.gene63735:intron 35.0%
!!! TTGGACGTTTTGGTTTCAAA+AGG + chr2.1:23563947-23563966 None:intergenic 35.0%
!!! TTTTGAAACCAAAACGTCCA+AGG - chr2.1:23563946-23563965 MS.gene63735:intron 35.0%
ATCAGTGAAGAACTTTCAGC+TGG - chr2.1:23564172-23564191 MS.gene63735:CDS 40.0%
ATGATATCCGCAAGAAGTTC+TGG + chr2.1:23562178-23562197 None:intergenic 40.0%
ATGCTTATGTTGGCAAGCTA+AGG - chr2.1:23563526-23563545 MS.gene63735:intron 40.0%
CAATTTACAACACAGTGGTG+GGG + chr2.1:23563192-23563211 None:intergenic 40.0%
CAGATACTTCAACAATGCTC+AGG - chr2.1:23562081-23562100 MS.gene63735:CDS 40.0%
CCAATTTACAACACAGTGGT+GGG + chr2.1:23563193-23563212 None:intergenic 40.0%
CTGTCATATCCTTTCTCTCA+TGG + chr2.1:23564148-23564167 None:intergenic 40.0%
CTTCACAATTTATGATGCCC+CGG - chr2.1:23563806-23563825 MS.gene63735:intron 40.0%
CTTGAACTTCCATGAGAGAA+AGG - chr2.1:23564136-23564155 MS.gene63735:CDS 40.0%
GCCAAAGCTAGTTAAGCAAA+TGG + chr2.1:23564330-23564349 None:intergenic 40.0%
GCTATCTGAACTTTCGCATA+TGG + chr2.1:23564006-23564025 None:intergenic 40.0%
GGCAAGATAGAGGTAAAATG+TGG + chr2.1:23563229-23563248 None:intergenic 40.0%
TAAGGGCTGTCTATAGTACA+TGG + chr2.1:23564288-23564307 None:intergenic 40.0%
TAATATTCACCCCTTTGTCC+GGG + chr2.1:23563646-23563665 None:intergenic 40.0%
TAGTGGCTATCTATGAAGCA+TGG - chr2.1:23562472-23562491 MS.gene63735:CDS 40.0%
TATGAAGCACAAACACACCT+CGG + chr2.1:23562640-23562659 None:intergenic 40.0%
TCCAATTTACAACACAGTGG+TGG + chr2.1:23563194-23563213 None:intergenic 40.0%
TGAACTTTCGCATATGGATG+AGG + chr2.1:23564000-23564019 None:intergenic 40.0%
TGAAGAATAAAGCTCCGATG+TGG - chr2.1:23564090-23564109 MS.gene63735:CDS 40.0%
TGAGTTTAAACCCGGACAAA+GGG - chr2.1:23563633-23563652 MS.gene63735:intron 40.0%
TTAAGCAAATGGCAAACGCA+TGG + chr2.1:23564319-23564338 None:intergenic 40.0%
TTCCCTGTATCTGTTAATGC+TGG + chr2.1:23563417-23563436 None:intergenic 40.0%
TTGAGTTTAAACCCGGACAA+AGG - chr2.1:23563632-23563651 MS.gene63735:intron 40.0%
TTTGTCCGGGTTTAAACTCA+AGG + chr2.1:23563633-23563652 None:intergenic 40.0%
! AGGACCTGTTTAAGACAAGA+AGG + chr2.1:23562337-23562356 None:intergenic 40.0%
! GCCATTTGCTTAACTAGCTT+TGG - chr2.1:23564326-23564345 MS.gene63735:CDS 40.0%
! GGACCTGTTTAAGACAAGAA+GGG + chr2.1:23562336-23562355 None:intergenic 40.0%
! TGTTCTGTCACTTGTTGGTT+TGG + chr2.1:23563838-23563857 None:intergenic 40.0%
!! CTCCAGTTTTGTAAAGTAGG+GGG - chr2.1:23564251-23564270 MS.gene63735:CDS 40.0%
!!! ACTCTCGATTTTGACTACCT+TGG - chr2.1:23563132-23563151 MS.gene63735:intron 40.0%
AAACGCATGGAAGGCAGATA+AGG + chr2.1:23564306-23564325 None:intergenic 45.0%
AACGCATGGAAGGCAGATAA+GGG + chr2.1:23564305-23564324 None:intergenic 45.0%
AGAGAGCAAATCATTCGCCT+TGG + chr2.1:23563966-23563985 None:intergenic 45.0%
ATGAAACCAAGTTACTCGGC+TGG - chr2.1:23562837-23562856 MS.gene63735:intron 45.0%
ATGAAGCACAAACACACCTC+GGG + chr2.1:23562639-23562658 None:intergenic 45.0%
ATGAAGCATGGATACGGACA+CGG - chr2.1:23562484-23562503 MS.gene63735:CDS 45.0%
ATGGTGTAAGGGCAAGATAG+AGG + chr2.1:23563239-23563258 None:intergenic 45.0%
CAACAAGTGACAGAACAAGC+CGG - chr2.1:23563841-23563860 MS.gene63735:intron 45.0%
CACCCCCTACTTTACAAAAC+TGG + chr2.1:23564256-23564275 None:intergenic 45.0%
CATGCTTCCAGAACTTCTTG+CGG - chr2.1:23562168-23562187 MS.gene63735:CDS 45.0%
CCCACCACTGTGTTGTAAAT+TGG - chr2.1:23563190-23563209 MS.gene63735:CDS 45.0%
CGCCAGCATTAACAGATACA+GGG - chr2.1:23563412-23563431 MS.gene63735:intron 45.0%
GAGGACCTTGAGTTTAAACC+CGG - chr2.1:23563625-23563644 MS.gene63735:intron 45.0%
GAGGTGTGTTTGTGCTTCAT+AGG - chr2.1:23562639-23562658 MS.gene63735:intron 45.0%
GAGTTTAAACCCGGACAAAG+GGG - chr2.1:23563634-23563653 MS.gene63735:intron 45.0%
GCTGGAAGTCATCAAAACCA+AGG + chr2.1:23563152-23563171 None:intergenic 45.0%
GTGTGTTTGTGCTTCATAGG+TGG - chr2.1:23562642-23562661 MS.gene63735:intron 45.0%
TACAAGTGTCAGTGTCGTGT+CGG - chr2.1:23562573-23562592 MS.gene63735:CDS 45.0%
TCCAATTGCTCGTTCCACAT+CGG + chr2.1:23564107-23564126 None:intergenic 45.0%
TCGCCAGCATTAACAGATAC+AGG - chr2.1:23563411-23563430 MS.gene63735:intron 45.0%
TCTGTTTGCTTGCAAAACGC+CGG + chr2.1:23563863-23563882 None:intergenic 45.0%
TTGATGTCACGGGAGAACAA+TGG - chr2.1:23562206-23562225 MS.gene63735:CDS 45.0%
! ATCATTCGCCTTGGACGTTT+TGG + chr2.1:23563957-23563976 None:intergenic 45.0%
! GATGCCGATGACTTTTCACA+AGG - chr2.1:23563498-23563517 MS.gene63735:intron 45.0%
!! AACTAGCTTTGGCACCAAAC+TGG - chr2.1:23564337-23564356 MS.gene63735:CDS 45.0%
!! GTTTTGATGACTTCCAGCCA+GGG - chr2.1:23563154-23563173 MS.gene63735:intron 45.0%
ACAATTTATGATGCCCCGGC+TGG - chr2.1:23563810-23563829 MS.gene63735:intron 50.0%
ACGTTACCAGCAGGAACTTG+AGG + chr2.1:23563891-23563910 None:intergenic 50.0%
ACTGTGGATCAAAGCCAACC+AGG - chr2.1:23564200-23564219 MS.gene63735:CDS 50.0%
ATAAGCCCTCAAGTTCCTGC+TGG - chr2.1:23563882-23563901 MS.gene63735:intron 50.0%
ATATCACACGTGAGGTCGTC+GGG - chr2.1:23562281-23562300 MS.gene63735:CDS 50.0%
CGTTACCAGCAGGAACTTGA+GGG + chr2.1:23563890-23563909 None:intergenic 50.0%
CTTGTGCCTGCGTTTGCAAA+CGG - chr2.1:23562251-23562270 MS.gene63735:CDS 50.0%
CTTTCGCATATGGATGAGGC+AGG + chr2.1:23563996-23564015 None:intergenic 50.0%
GATATCACACGTGAGGTCGT+CGG - chr2.1:23562280-23562299 MS.gene63735:CDS 50.0%
GCAAATGGCAAACGCATGGA+AGG + chr2.1:23564315-23564334 None:intergenic 50.0%
GTCGGGTCACCTTCTCTTAA+CGG - chr2.1:23562298-23562317 MS.gene63735:CDS 50.0%
GTGGAGAGATATCACACGTG+AGG - chr2.1:23562273-23562292 MS.gene63735:CDS 50.0%
TCCGATGTGGAACGAGCAAT+TGG - chr2.1:23564103-23564122 MS.gene63735:CDS 50.0%
TGACACCACCGATAGTGCAT+CGG - chr2.1:23562123-23562142 MS.gene63735:CDS 50.0%
TGGATCAAAGCCAACCAGGA+GGG - chr2.1:23564204-23564223 MS.gene63735:CDS 50.0%
! CGGCTTGTTCTGTCACTTGT+TGG + chr2.1:23563843-23563862 None:intergenic 50.0%
! TTACAACACAGTGGTGGGGA+AGG + chr2.1:23563188-23563207 None:intergenic 50.0%
!! ACTATCGGTGGTGTCATCTC+CGG + chr2.1:23562119-23562138 None:intergenic 50.0%
!! CGCAAGAAGTTCTGGAAGCA+TGG + chr2.1:23562170-23562189 None:intergenic 50.0%
!! GGTTTTGATGACTTCCAGCC+AGG - chr2.1:23563153-23563172 MS.gene63735:intron 50.0%
!! TGGTTGGCTTTGATCCACAG+TGG + chr2.1:23564201-23564220 None:intergenic 50.0%
ACCGATAGTGCATCGGAGGA+CGG - chr2.1:23562130-23562149 MS.gene63735:CDS 55.0%
ACGAGTCCAGCCGAGTAACT+TGG + chr2.1:23562846-23562865 None:intergenic 55.0%
ATACGGACACGGACACGACA+CGG - chr2.1:23562495-23562514 MS.gene63735:CDS 55.0%
CATCGGAGGACGGAACAGAT+TGG - chr2.1:23562140-23562159 MS.gene63735:CDS 55.0%
CTTTCAGCTGGTTGCCACTG+TGG - chr2.1:23564184-23564203 MS.gene63735:CDS 55.0%
GCGACGACATTTCGACGATG+CGG + chr2.1:23562232-23562251 None:intergenic 55.0%
GCTCAGGCTCTCAATGCTAC+CGG - chr2.1:23562097-23562116 MS.gene63735:CDS 55.0%
GGAACGAGCAATTGGAGTGC+TGG - chr2.1:23564111-23564130 MS.gene63735:CDS 55.0%
GTGGATCAAAGCCAACCAGG+AGG - chr2.1:23564203-23564222 MS.gene63735:CDS 55.0%
TCCGTCCTCCGATGCACTAT+CGG + chr2.1:23562134-23562153 None:intergenic 55.0%
TCTCCACCGTTTGCAAACGC+AGG + chr2.1:23562260-23562279 None:intergenic 55.0%
TCTTCATCTCCTCTCCCTCC+TGG + chr2.1:23564221-23564240 None:intergenic 55.0%
! AGTTCCTGCTGGTAACGTCG+AGG - chr2.1:23563893-23563912 MS.gene63735:intron 55.0%
! TGTCAGTGTCGTGTCGGTGT+CGG - chr2.1:23562579-23562598 MS.gene63735:CDS 55.0%
!! CTTGTTGGTTTGGCACCAGC+CGG + chr2.1:23563828-23563847 None:intergenic 55.0%
!! TTGTTGGTTTGGCACCAGCC+GGG + chr2.1:23563827-23563846 None:intergenic 55.0%
CAAAGCCAACCAGGAGGGAG+AGG - chr2.1:23564209-23564228 MS.gene63735:CDS 60.0%
CACCACCGATAGTGCATCGG+AGG - chr2.1:23562126-23562145 MS.gene63735:CDS 60.0%
CATCTCCTCTCCCTCCTGGT+TGG + chr2.1:23564217-23564236 None:intergenic 60.0%
GGTTAGACGTGTCGCGGTGT+CGG + chr2.1:23562618-23562637 None:intergenic 60.0%
GTCCTCCGATGCACTATCGG+TGG + chr2.1:23562131-23562150 None:intergenic 60.0%
GTGCCTGCGTTTGCAAACGG+TGG - chr2.1:23562254-23562273 MS.gene63735:CDS 60.0%
! ACCTCGGGTTAGACGTGTCG+CGG + chr2.1:23562624-23562643 None:intergenic 60.0%
! CTGCTGGTAACGTCGAGGCA+GGG - chr2.1:23563898-23563917 MS.gene63735:intron 60.0%
!! TGTTGGTTTGGCACCAGCCG+GGG + chr2.1:23563826-23563845 None:intergenic 60.0%
ACCGCGACACGTCTAACCCG+AGG - chr2.1:23562620-23562639 MS.gene63735:intron 65.0%
CCTGCCTCGACGTTACCAGC+AGG + chr2.1:23563900-23563919 None:intergenic 65.0%
! CCTGCTGGTAACGTCGAGGC+AGG - chr2.1:23563897-23563916 MS.gene63735:intron 65.0%
! CAGTGGTGGGGAAGGTCGCG+TGG + chr2.1:23563180-23563199 None:intergenic 70.0%
GAAGGTCGCGTGGCCCTGGC+TGG + chr2.1:23563170-23563189 None:intergenic 75.0%
TGGGGAAGGTCGCGTGGCCC+TGG + chr2.1:23563174-23563193 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 23562052 23564370 23562052 ID=MS.gene63735
chr2.1 mRNA 23562052 23564370 23562052 ID=MS.gene63735.t1;Parent=MS.gene63735
chr2.1 exon 23564071 23564370 23564071 ID=MS.gene63735.t1.exon1;Parent=MS.gene63735.t1
chr2.1 CDS 23564071 23564370 23564071 ID=cds.MS.gene63735.t1;Parent=MS.gene63735.t1
chr2.1 exon 23563164 23563251 23563164 ID=MS.gene63735.t1.exon2;Parent=MS.gene63735.t1
chr2.1 CDS 23563164 23563251 23563164 ID=cds.MS.gene63735.t1;Parent=MS.gene63735.t1
chr2.1 exon 23562875 23563004 23562875 ID=MS.gene63735.t1.exon3;Parent=MS.gene63735.t1
chr2.1 CDS 23562875 23563004 23562875 ID=cds.MS.gene63735.t1;Parent=MS.gene63735.t1
chr2.1 exon 23562052 23562637 23562052 ID=MS.gene63735.t1.exon4;Parent=MS.gene63735.t1
chr2.1 CDS 23562052 23562637 23562052 ID=cds.MS.gene63735.t1;Parent=MS.gene63735.t1
Gene Sequence

>MS.gene63735

ATGAAGCTCTTTCGATCTCTTAAATTCTTCAGATACTTCAACAATGCTCAGGCTCTCAATGCTACCGGAGATGACACCACCGATAGTGCATCGGAGGACGGAACAGATTGGTCTACCATGCTTCCAGAACTTCTTGCGGATATCATTAAACGAGTTGATGTCACGGGAGAACAATGGCCGCATCGTCGAAATGTCGTCGCTTGTGCCTGCGTTTGCAAACGGTGGAGAGATATCACACGTGAGGTCGTCGGGTCACCTTCTCTTAACGGAAAAATCACTTTCCCTTCTTGTCTTAAACAGGTCCTGCAACAAAATGAATTCATTTTATTTTTCTCTCTTGAATAATTTATGGATCATGAAAAATTTGGATGTAGTAATACATTATTTTTCTTCATAATGAAGACTAACATTTTGTTCAATTAGTGGCTATCTATGAAGCATGGATACGGACACGGACACGACACGGACACTGACACACCGACACAACTAATAATTTGAAAATTCAGATAATTCAGTGTAATTACAAGTGTCAGTGTCGTGTCGGTGTCGGACACGACACGTGTCCGACACCGCGACACGTCTAACCCGAGGTGTGTTTGTGCTTCATAGGTGGCTATTAAGTGTCTGATAATGAGACATCAATTGAATTAAGAGAGTTGGAGTTTGACAATCTTGTTATGTTTTGATAAAGGTTGTTAAAATCACGAGTTAACTCGTAGGATCATATGTTTAGGTATCAATTTTGAGTTGACTCGACTATAAACTACAATTAAGTTAACTCGAAAATGAAACCAAGTTACTCGGCTGGACTCGTAGTGAATTTGTTAACTCTTAAACTTTCAGAAATTTTTTGAAGAACATTTTAATCTCTTCTTTTTTCATCTCAATTTTCAAACTCAATTCCTTTTCTTCTTTAATAGTTTGATTTTGTTCATATCAATTTTTTCTTTTGAATTGAGTTAAAATTTTGAATTATTGATGCATTTATTAAGTATATAGGTCATATATATTATTTTAATATTAGGCAAACTCGTACAAGTCTACGAGTTAATTTCACGTAGGCAAATTAAGTTTACTTAAACTCTCGATTTTGACTACCTTGGTTTTGATGACTTCCAGCCAGGGCCACGCGACCTTCCCCACCACTGTGTTGTAAATTGGAATAAGAAGACCTCCACATTTTACCTCTATCTTGCCCTTACACCATGTAAGTAAGTACTGTTTTGGCAAAAGGATTATTTGGTTATAACTATAATAATGATTAAAATTTCAAATGGAGATTGGACAATGCTTATTTTTCAAAATATTTCAACAAATATAACTTGATGATGTTGCACAACTTATGATTTCTTTTTTCAATCGCCAGCATTAACAGATACAGGGAAATTCCTATTAGCAGCTAGAAGATATCGGTGTGTTACGCATACTGAGTATGTTATATCGCTCGATGCCGATGACTTTTCACAAGGAAGTAATGCTTATGTTGGCAAGCTAAGGTAAATATTTCTTGTAACAGAAACTAAAATGATCCTCATTCAGGAAAGAAAAACCATTTATTGAAGAAAAAGGGTAGGAGGACCTTGAGTTTAAACCCGGACAAAGGGGTGAATATTAATCTAACAAACTAACTAATAACATCTGGCATTAAAAAAAACCATTTATTGAATTTATTCTCAACAATCTTTTGCTTTGTCTGGAGTACTGATCATTTAGTTGCTGAAATGTCTTCACAGTTCAGATTTTCTTGAAGCAAGCTTCACAATTTATGATGCCCCGGCTGGTGCCAAACCAACAAGTGACAGAACAAGCCGGCGTTTTGCAAGCAAACAGATAAGCCCTCAAGTTCCTGCTGGTAACGTCGAGGCAGGGAACATCTCCTACAAAAAATTCAGCCTTTTGAAACCAAAACGTCCAAGGCGAATGATTTGCTCTCTGAAGTCTCCTGCCTCATCCATATGCGAAAGTTCAGATAGCAAGTCTTTCAACAATCATAAGATGCACTGTAAAGAACAAGCTACTTCTTCTGCCTACACAATCTTGAAGAATAAAGCTCCGATGTGGAACGAGCAATTGGAGTGCTGGTTCTTGAACTTCCATGAGAGAAAGGATATGACAGTAGCATCAGTGAAGAACTTTCAGCTGGTTGCCACTGTGGATCAAAGCCAACCAGGAGGGAGAGGAGATGAAGAAACAGTTCTTCTCCAGTTTTGTAAAGTAGGGGGTGATACTTTCACCATGTACTATAGACAGCCCTTATCTGCCTTCCATGCGTTTGCCATTTGCTTAACTAGCTTTGGCACCAAACTGGCTTGTCAGTAA
Protein sequence

>MS.gene63735.t1

MKLFRSLKFFRYFNNAQALNATGDDTTDSASEDGTDWSTMLPELLADIIKRVDVTGEQWPHRRNVVACACVCKRWRDITREVVGSPSLNGKITFPSCLKQPGPRDLPHHCVVNWNKKTSTFYLYLALTPSLTDTGKFLLAARRYRCVTHTEYVISLDADDFSQGSNAYVGKLSSDFLEASFTIYDAPAGAKPTSDRTSRRFASKQISPQVPAGNVEAGNISYKKFSLLKPKRPRRMICSLKSPASSICESSDSKSFNNHKMHCKEQATSSAYTILKNKAPMWNEQLECWFLNFHERKDMTVASVKNFQLVATVDQSQPGGRGDEETVLLQFCKVGGDTFTMYYRQPLSAFHAFAICLTSFGTKLACQ