Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64888.t1 | XP_003612368.2 | 95.6 | 1232 | 53 | 1 | 1 | 1231 | 1 | 1232 | 0.00E+00 | 2343.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64888.t1 | F4JN26 | 36.9 | 1295 | 613 | 27 | 1 | 1231 | 1 | 1155 | 2.4e-205 | 717.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64888.t1 | G7K187 | 95.6 | 1232 | 53 | 1 | 1 | 1231 | 1 | 1232 | 0.0e+00 | 2343.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene96593 | MS.gene64888 | PPI |
| MS.gene009300 | MS.gene64888 | PPI |
| MS.gene64888 | MS.gene52850 | PPI |
| MS.gene058795 | MS.gene64888 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64888.t1 | MTR_5g024250 | 95.373 | 1232 | 56 | 1 | 1 | 1231 | 1 | 1232 | 0.0 | 2433 |
| MS.gene64888.t1 | MTR_1g088720 | 25.067 | 371 | 243 | 9 | 4 | 367 | 8 | 350 | 3.31e-25 | 114 |
| MS.gene64888.t1 | MTR_1g088720 | 27.434 | 226 | 129 | 5 | 986 | 1194 | 719 | 926 | 9.67e-11 | 67.0 |
| MS.gene64888.t1 | MTR_3g005810 | 31.159 | 276 | 162 | 8 | 4 | 264 | 7 | 269 | 6.11e-22 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64888.t1 | AT4G35520 | 54.048 | 420 | 171 | 10 | 818 | 1231 | 528 | 931 | 2.86e-129 | 420 |
| MS.gene64888.t1 | AT4G35520 | 36.686 | 169 | 97 | 2 | 259 | 418 | 8 | 175 | 7.22e-25 | 112 |
| MS.gene64888.t1 | AT4G35520 | 50.585 | 427 | 199 | 3 | 1 | 418 | 1 | 424 | 4.31e-129 | 426 |
| MS.gene64888.t1 | AT4G35520 | 54.306 | 418 | 169 | 10 | 820 | 1231 | 779 | 1180 | 1.46e-127 | 422 |
| MS.gene64888.t1 | AT4G35520 | 54.306 | 418 | 169 | 10 | 820 | 1231 | 754 | 1155 | 8.39e-128 | 422 |
| MS.gene64888.t1 | AT4G35520 | 48.011 | 377 | 157 | 1 | 1 | 377 | 1 | 338 | 6.06e-103 | 353 |
| MS.gene64888.t1 | AT4G35520 | 54.306 | 418 | 169 | 10 | 820 | 1231 | 742 | 1143 | 8.90e-128 | 422 |
| MS.gene64888.t1 | AT4G35520 | 44.965 | 427 | 186 | 3 | 1 | 418 | 1 | 387 | 1.95e-104 | 357 |
| MS.gene64888.t1 | AT4G35520 | 54.306 | 418 | 169 | 10 | 820 | 1231 | 752 | 1153 | 1.18e-127 | 422 |
| MS.gene64888.t1 | AT4G35520 | 57.429 | 350 | 147 | 1 | 1 | 350 | 1 | 348 | 2.79e-127 | 421 |
| MS.gene64888.t1 | AT4G35520 | 52.304 | 434 | 171 | 11 | 818 | 1231 | 540 | 957 | 2.66e-125 | 410 |
| MS.gene64888.t1 | AT4G35520 | 42.857 | 119 | 68 | 0 | 259 | 377 | 8 | 126 | 1.65e-23 | 108 |
| MS.gene64888.t1 | AT4G35520 | 52.546 | 432 | 169 | 11 | 820 | 1231 | 742 | 1157 | 4.90e-124 | 412 |
| MS.gene64888.t1 | AT4G35520 | 44.965 | 427 | 186 | 3 | 1 | 418 | 1 | 387 | 3.20e-104 | 357 |
| MS.gene64888.t1 | AT4G35520 | 44.965 | 427 | 186 | 3 | 1 | 418 | 1 | 387 | 1.29e-104 | 356 |
| MS.gene64888.t1 | AT4G35520 | 49.711 | 346 | 157 | 9 | 820 | 1159 | 742 | 1076 | 4.03e-87 | 306 |
| MS.gene64888.t1 | AT4G02460 | 26.733 | 404 | 230 | 14 | 4 | 386 | 17 | 375 | 6.88e-24 | 109 |
| MS.gene64888.t1 | AT4G02460 | 26.733 | 404 | 230 | 14 | 4 | 386 | 17 | 375 | 9.30e-24 | 109 |
| MS.gene64888.t1 | AT4G09140 | 32.857 | 280 | 152 | 10 | 4 | 264 | 29 | 291 | 2.96e-23 | 107 |
Find 256 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCATATATCTTGAATTTA+AGG | 0.086807 | 5.3:-14092652 | MS.gene64888:CDS |
| TACATCGAAATTTCTTAATT+TGG | 0.131266 | 5.3:-14094572 | MS.gene64888:CDS |
| ACAACTTTGAAGGAGATATT+TGG | 0.162274 | 5.3:+14093323 | None:intergenic |
| TGACTATCTTTGCAGGAATT+TGG | 0.205955 | 5.3:-14086334 | MS.gene64888:intron |
| ATTGATTGAAGTACCTTATT+GGG | 0.214424 | 5.3:+14093380 | None:intergenic |
| GATTGATTGAAGTACCTTAT+TGG | 0.215758 | 5.3:+14093379 | None:intergenic |
| GTGCAAGATTCCGGAGATTA+TGG | 0.216270 | 5.3:-14087927 | MS.gene64888:CDS |
| CGGTTATTCTGGCAATTTAC+TGG | 0.226743 | 5.3:-14089993 | MS.gene64888:CDS |
| CACTTGTAACCACTGGCTTT+GGG | 0.232639 | 5.3:-14093170 | MS.gene64888:CDS |
| TTGAATATTTAGGTATTATC+TGG | 0.241070 | 5.3:-14086848 | MS.gene64888:intron |
| GACCCTGCTTCCAAACTTCT+AGG | 0.247169 | 5.3:-14088759 | MS.gene64888:intron |
| ATCAACTACTTCATAGTTAC+AGG | 0.248733 | 5.3:-14086675 | MS.gene64888:CDS |
| AACTCCAAACCGATAGCTTT+TGG | 0.249668 | 5.3:-14089619 | MS.gene64888:CDS |
| TCACTTGTAACCACTGGCTT+TGG | 0.249770 | 5.3:-14093171 | MS.gene64888:CDS |
| TCTGGATTCTTGCATCTTGC+TGG | 0.260504 | 5.3:-14087691 | MS.gene64888:CDS |
| CTTCGTCAGACCGGTTATTC+TGG | 0.261638 | 5.3:-14090004 | MS.gene64888:CDS |
| TCTTAAGGTGCGATTATGTT+TGG | 0.264068 | 5.3:-14085762 | MS.gene64888:intron |
| CATTTCTTACTGATAGTATC+AGG | 0.266001 | 5.3:+14087655 | None:intergenic |
| TTCAACAAAATGATCCATTC+AGG | 0.266010 | 5.3:-14088387 | MS.gene64888:CDS |
| AATATACTTGACATCTCTTC+TGG | 0.266206 | 5.3:-14087709 | MS.gene64888:CDS |
| CTTAAGGTGCGATTATGTTT+GGG | 0.269942 | 5.3:-14085761 | MS.gene64888:intron |
| CCTCAAAGAGAAACAAAATA+AGG | 0.270902 | 5.3:-14090035 | MS.gene64888:CDS |
| GTTTCAGTGTTTGTTGATAT+TGG | 0.273741 | 5.3:-14095685 | MS.gene64888:CDS |
| CATTTCTGAAGTCTCTTTGT+TGG | 0.291486 | 5.3:-14094494 | MS.gene64888:CDS |
| AACGATATCATAAAGCTTAC+TGG | 0.297572 | 5.3:-14093108 | MS.gene64888:CDS |
| CATAGCGGTGGACATGCTTT+TGG | 0.298068 | 5.3:+14089958 | None:intergenic |
| AAAAGGTCATCAAACAGTTC+TGG | 0.303292 | 5.3:-14090598 | MS.gene64888:CDS |
| GAAACGGAGTTCAGAGGTTC+TGG | 0.308935 | 5.3:-14088464 | MS.gene64888:CDS |
| AACAGACCCAACATTATAAA+AGG | 0.310471 | 5.3:-14088939 | MS.gene64888:CDS |
| TCCAATAGATCTTCATTGTT+GGG | 0.311658 | 5.3:+14088601 | None:intergenic |
| CTAAACAATCCTTACGATCT+TGG | 0.315143 | 5.3:+14090164 | None:intergenic |
| CAAGCAAAGTCTTCTCGTTC+AGG | 0.317110 | 5.3:+14089657 | None:intergenic |
| CCCCAACAATGAAGATCTAT+TGG | 0.321390 | 5.3:-14088602 | MS.gene64888:CDS |
| TTCTAACACACTCAGGTAAT+AGG | 0.325451 | 5.3:+14088967 | None:intergenic |
| CTCCAACAAAGCCACTTATA+TGG | 0.326027 | 5.3:-14090457 | MS.gene64888:CDS |
| ATGATACACATTTCACAAAA+AGG | 0.328678 | 5.3:-14089019 | MS.gene64888:CDS |
| CTACGAACAGATTCCGATAA+AGG | 0.341565 | 5.3:+14095889 | None:intergenic |
| CCGGAGATTATGGGAATAAA+TGG | 0.344536 | 5.3:-14087917 | MS.gene64888:CDS |
| ACCAATGATATTAATGATCT+TGG | 0.347298 | 5.3:-14089326 | MS.gene64888:CDS |
| CACTGTTCCTTTAGTCAACT+TGG | 0.348856 | 5.3:-14085569 | MS.gene64888:CDS |
| TTGTTTCAGAATTGATATCT+AGG | 0.349287 | 5.3:-14087953 | MS.gene64888:intron |
| CAACTTTGAAGGAGATATTT+GGG | 0.351489 | 5.3:+14093324 | None:intergenic |
| TTCAATATCAACAACTTTGA+AGG | 0.351846 | 5.3:+14093313 | None:intergenic |
| CATGCAGATATATCAAAGTT+TGG | 0.352223 | 5.3:-14090190 | MS.gene64888:intron |
| TCCAAGATCATTAATATCAT+TGG | 0.357505 | 5.3:+14089325 | None:intergenic |
| GCATACATCCCTCTGTTTCC+AGG | 0.358909 | 5.3:-14085691 | MS.gene64888:intron |
| CAACATTATAAAAGGCGAAT+TGG | 0.362809 | 5.3:-14088931 | MS.gene64888:CDS |
| GAAATAATGACAAGAACTTA+TGG | 0.365886 | 5.3:-14094472 | MS.gene64888:CDS |
| CTCCTTCTACTTCTATGAAA+TGG | 0.368433 | 5.3:+14088843 | None:intergenic |
| GCTTTAGCTCTTCAACTAGA+AGG | 0.372548 | 5.3:+14085712 | None:intergenic |
| TGGATTTGTTTCCTCCGAAC+TGG | 0.374132 | 5.3:+14093536 | None:intergenic |
| AAAATTCTGAATCCTTTAGA+AGG | 0.374608 | 5.3:-14086609 | MS.gene64888:intron |
| TTTACCTGCTTTGGCTGAAA+TGG | 0.374834 | 5.3:+14090401 | None:intergenic |
| CAACAAAGAGACTTCAGAAA+TGG | 0.375063 | 5.3:+14094495 | None:intergenic |
| AACCTATCTAGATGCTGAAC+AGG | 0.381349 | 5.3:-14086810 | MS.gene64888:CDS |
| TCTAGGGAAGTGCAAGATTC+CGG | 0.388056 | 5.3:-14087936 | MS.gene64888:CDS |
| AATACTTTCCTATATCCATT+TGG | 0.389721 | 5.3:+14094445 | None:intergenic |
| CAGTAAATTGCCAGAATAAC+CGG | 0.392348 | 5.3:+14089994 | None:intergenic |
| AACCATATAAGTGGCTTTGT+TGG | 0.392990 | 5.3:+14090455 | None:intergenic |
| CAGGGAACAGATTAAAGATT+GGG | 0.394408 | 5.3:-14086656 | MS.gene64888:CDS |
| CAGGTCCCTTGCAAACGAAC+TGG | 0.395404 | 5.3:+14092780 | None:intergenic |
| AACCCTTGCTGTTATTGATC+AGG | 0.398168 | 5.3:-14087569 | MS.gene64888:intron |
| AATATTATCATAGAAACTTT+CGG | 0.404601 | 5.3:+14089853 | None:intergenic |
| ATCGAAATTTCTTAATTTGG+CGG | 0.404648 | 5.3:-14094569 | MS.gene64888:CDS |
| TACTCGAGATGGGCTGGAAT+TGG | 0.405515 | 5.3:-14095362 | MS.gene64888:CDS |
| ATTGTCTCCCTCTAGATTCA+TGG | 0.408060 | 5.3:-14089463 | MS.gene64888:CDS |
| CTGAATTGGAAGCCCCAATA+AGG | 0.417478 | 5.3:-14093393 | MS.gene64888:intron |
| AGAAAGGAAGTAGGCACAAC+AGG | 0.420276 | 5.3:-14094287 | MS.gene64888:intron |
| AACCACTGGCTTTGGGGTGG+AGG | 0.425969 | 5.3:-14093163 | MS.gene64888:CDS |
| TACTCCAAAAGCTATCGGTT+TGG | 0.428894 | 5.3:+14089615 | None:intergenic |
| TAAAGAAATATTCAACAGAT+TGG | 0.428963 | 5.3:-14089060 | MS.gene64888:CDS |
| ATGACAATCATCAGTGCTAC+AGG | 0.429264 | 5.3:-14090105 | MS.gene64888:CDS |
| AGGGTTCTGCCAGCCATGAT+TGG | 0.431893 | 5.3:+14087586 | None:intergenic |
| CATGTCAAGGAATGCTAACC+AGG | 0.437046 | 5.3:-14089141 | MS.gene64888:CDS |
| GGCGACGATAAGACCTTTAT+CGG | 0.438204 | 5.3:-14095902 | MS.gene64888:CDS |
| CTTAAATTCAAGATATATGC+AGG | 0.440812 | 5.3:+14092653 | None:intergenic |
| AAGCTTACTGGATACATATC+AGG | 0.441992 | 5.3:-14093096 | MS.gene64888:CDS |
| TTCCAATAGATCTTCATTGT+TGG | 0.442789 | 5.3:+14088600 | None:intergenic |
| TTCATTTAGCTTGTCTGAAG+TGG | 0.442895 | 5.3:+14090129 | None:intergenic |
| ATGGACGGCCAAATGGATAT+AGG | 0.443464 | 5.3:-14094453 | MS.gene64888:CDS |
| TTTAAATCAGCCTCCCGAAA+TGG | 0.444086 | 5.3:-14088811 | MS.gene64888:CDS |
| ATGCAGGTACTGCCAGAGAT+TGG | 0.445165 | 5.3:-14086700 | MS.gene64888:intron |
| CTCCATTTCATAGAAGTAGA+AGG | 0.445805 | 5.3:-14088845 | MS.gene64888:CDS |
| TTCATTGTGCAGTGTGCTCA+TGG | 0.448403 | 5.3:-14085601 | MS.gene64888:intron |
| CACTCAGGTAATAGGCAGTC+TGG | 0.454013 | 5.3:+14088975 | None:intergenic |
| TCATAGAAACTTTCGGCAGC+AGG | 0.454172 | 5.3:+14089860 | None:intergenic |
| AAGAGAAAAGCAACCTGGAT+TGG | 0.454580 | 5.3:+14093516 | None:intergenic |
| GTAGAGCAACTCTTTAGAAA+AGG | 0.454936 | 5.3:+14089695 | None:intergenic |
| GGTTATGAGTGCCATGGGTT+CGG | 0.455266 | 5.3:-14089752 | MS.gene64888:CDS |
| TGGTATTCAGCAACACAAAC+AGG | 0.455374 | 5.3:-14089443 | MS.gene64888:CDS |
| TGCAAGATTCCGGAGATTAT+GGG | 0.455509 | 5.3:-14087926 | MS.gene64888:CDS |
| TGACTTAAAAGCTTATGAAC+AGG | 0.455665 | 5.3:+14092761 | None:intergenic |
| TTGTTGGGGCTAAGAGCATC+AGG | 0.457417 | 5.3:+14088616 | None:intergenic |
| GCAACACAAACAGGTAACTT+TGG | 0.459066 | 5.3:-14089434 | MS.gene64888:CDS |
| AAAGGTCATCAAACAGTTCT+GGG | 0.460494 | 5.3:-14090597 | MS.gene64888:CDS |
| TATGAATCACCTTCATATCC+AGG | 0.462141 | 5.3:+14093061 | None:intergenic |
| AATGACGCTGCTGAGATTGA+AGG | 0.462435 | 5.3:-14088427 | MS.gene64888:CDS |
| TATTCAACAGATTGGTTGAC+TGG | 0.463602 | 5.3:-14089052 | MS.gene64888:CDS |
| ATCCACCTGATGAAGAACTT+TGG | 0.466886 | 5.3:+14087621 | None:intergenic |
| CTCAAAGAGAAACAAAATAA+GGG | 0.467320 | 5.3:-14090034 | MS.gene64888:CDS |
| TGGATATAGGAAAGTATTGA+AGG | 0.472768 | 5.3:-14094440 | MS.gene64888:intron |
| AGAACTTATGGACGGCCAAA+TGG | 0.473558 | 5.3:-14094460 | MS.gene64888:CDS |
| ATCTTCTTGAACCATATAAG+TGG | 0.476038 | 5.3:+14090446 | None:intergenic |
| ACAGGGAACAGATTAAAGAT+TGG | 0.476385 | 5.3:-14086657 | MS.gene64888:CDS |
| TGGAATTACTCGAGATGGGC+TGG | 0.478152 | 5.3:-14095368 | MS.gene64888:CDS |
| TCAAAGTTGTTGATATTGAA+AGG | 0.484148 | 5.3:-14093310 | MS.gene64888:intron |
| CATAATCAAATAGCCAAGCT+TGG | 0.487009 | 5.3:-14085538 | MS.gene64888:CDS |
| AAATGTGTATCATGGAAATC+AGG | 0.487329 | 5.3:+14089029 | None:intergenic |
| CTTTAGCTCTTCAACTAGAA+GGG | 0.488271 | 5.3:+14085713 | None:intergenic |
| AATACTAAACTTCATTGAAA+AGG | 0.489534 | 5.3:-14090615 | MS.gene64888:CDS |
| TTGCTTACTCCAAAAGCTAT+CGG | 0.489893 | 5.3:+14089610 | None:intergenic |
| GGATGCCAAAGTTCTTCATC+AGG | 0.491605 | 5.3:-14087626 | MS.gene64888:CDS |
| CATATAAGTGGCTTTGTTGG+AGG | 0.492033 | 5.3:+14090458 | None:intergenic |
| GATAACAATGATTTATTGCA+AGG | 0.492434 | 5.3:-14089908 | MS.gene64888:CDS |
| CTTGTTCATCAGTCCAAGCT+TGG | 0.495904 | 5.3:+14085525 | None:intergenic |
| CCTCGATGCTCGCGCTACAA+AGG | 0.496323 | 5.3:-14095800 | MS.gene64888:intron |
| ACCTGCTTTGGCTGAAATGG+TGG | 0.497376 | 5.3:+14090404 | None:intergenic |
| GAATTGGTGGGAGAAAGATA+TGG | 0.497867 | 5.3:-14095346 | MS.gene64888:intron |
| TCGAGATGGGCTGGAATTGG+TGG | 0.499643 | 5.3:-14095359 | MS.gene64888:CDS |
| CCACTGTCACTTGTAACCAC+TGG | 0.501918 | 5.3:-14093177 | MS.gene64888:CDS |
| AGGGTCGGATGCTAGACCGC+TGG | 0.502251 | 5.3:+14088776 | None:intergenic |
| CCATTTATTCCCATAATCTC+CGG | 0.503204 | 5.3:+14087917 | None:intergenic |
| ATCATAGAACTAATGTGAAA+CGG | 0.504755 | 5.3:-14088480 | MS.gene64888:intron |
| AACTTGAGGTGCATGGATCC+TGG | 0.505219 | 5.3:+14089123 | None:intergenic |
| ATCGGTTATCAAACTCTGAA+TGG | 0.506997 | 5.3:-14089403 | MS.gene64888:CDS |
| GTAACTTTGGTTCTGATGAT+CGG | 0.510715 | 5.3:-14089421 | MS.gene64888:CDS |
| CTCCTGTTCAGCATCTAGAT+AGG | 0.511106 | 5.3:+14086808 | None:intergenic |
| GGAGGTGGAATTACTCGAGA+TGG | 0.511633 | 5.3:-14095373 | MS.gene64888:CDS |
| AACAGATTAAAGATTGGGGC+TGG | 0.512814 | 5.3:-14086651 | MS.gene64888:CDS |
| CCAATAGATCTTCATTGTTG+GGG | 0.514398 | 5.3:+14088602 | None:intergenic |
| TTGAAGGTTATGAGTGCCAT+GGG | 0.514841 | 5.3:-14089757 | MS.gene64888:CDS |
| CAACTCCCAGTTCGTTTGCA+AGG | 0.515346 | 5.3:-14092786 | MS.gene64888:CDS |
| CTGTGAGATATACCTTCTAA+AGG | 0.516124 | 5.3:+14086597 | None:intergenic |
| GTTTGGAAGCCAAGATCGTA+AGG | 0.516892 | 5.3:-14090173 | MS.gene64888:CDS |
| GGGTGCAAGTGTATATATCT+TGG | 0.517916 | 5.3:-14094323 | MS.gene64888:CDS |
| GCTCAAAACATATAAAGAGC+GGG | 0.517968 | 5.3:+14092625 | None:intergenic |
| TTGCAATACCCTGGATATGA+AGG | 0.518092 | 5.3:-14093070 | MS.gene64888:intron |
| AAACCTAGAAGTTTGGAAGC+AGG | 0.519365 | 5.3:+14088756 | None:intergenic |
| TGCAACTTCTACACAGGGAC+GGG | 0.520833 | 5.3:+14089544 | None:intergenic |
| GGTTATGATGTAGAGATTGG+TGG | 0.521108 | 5.3:-14089731 | MS.gene64888:CDS |
| ACATTGAACTCACCCCTGAA+TGG | 0.521481 | 5.3:+14088373 | None:intergenic |
| TATTCTACAACCAACCAGTT+CGG | 0.522442 | 5.3:-14093550 | MS.gene64888:CDS |
| ACTGGAAGACTTGCGTCAAA+AGG | 0.523475 | 5.3:-14086931 | MS.gene64888:intron |
| AACTCCCAGTTCGTTTGCAA+GGG | 0.524126 | 5.3:-14092785 | MS.gene64888:CDS |
| AGTAGTTGATAACCAATCTC+TGG | 0.524732 | 5.3:+14086688 | None:intergenic |
| TTCGGTTATGATGTAGAGAT+TGG | 0.525279 | 5.3:-14089734 | MS.gene64888:CDS |
| TGTAACCACTGGCTTTGGGG+TGG | 0.527919 | 5.3:-14093166 | MS.gene64888:CDS |
| GAAGAACTTCTCGCATAAGA+AGG | 0.530402 | 5.3:+14089830 | None:intergenic |
| TGGCATTGATGATGATAGAA+AGG | 0.533656 | 5.3:-14094303 | MS.gene64888:CDS |
| GGGTCGGATGCTAGACCGCT+GGG | 0.537874 | 5.3:+14088777 | None:intergenic |
| TCCACTATTTGCAAAACTTG+AGG | 0.543285 | 5.3:+14089109 | None:intergenic |
| AAATTAAATGAAGAAGATGC+TGG | 0.543458 | 5.3:-14089797 | MS.gene64888:CDS |
| ATGGATACAGAGATACTGTA+AGG | 0.543591 | 5.3:+14092928 | None:intergenic |
| GCCACCATTTCAGCCAAAGC+AGG | 0.544008 | 5.3:-14090405 | MS.gene64888:intron |
| TCAACAAAATGATCCATTCA+GGG | 0.544139 | 5.3:-14088386 | MS.gene64888:CDS |
| TGTTTCAGAATTGATATCTA+GGG | 0.546621 | 5.3:-14087952 | MS.gene64888:intron |
| TTTGCAAAACTTGAGGTGCA+TGG | 0.547477 | 5.3:+14089116 | None:intergenic |
| ACTTGTAACCACTGGCTTTG+GGG | 0.548380 | 5.3:-14093169 | MS.gene64888:CDS |
| AAATTGACACCTAAAATACA+TGG | 0.549038 | 5.3:+14086164 | None:intergenic |
| TCTAGATGCTGAACAGGAGC+TGG | 0.549469 | 5.3:-14086804 | MS.gene64888:intron |
| AAATTCATTCCAATCATGGC+TGG | 0.550857 | 5.3:-14087595 | MS.gene64888:CDS |
| ATCATCAACAATGCCACCCT+CGG | 0.553332 | 5.3:-14085990 | MS.gene64888:CDS |
| GTGATCTGTGAATCATTTGA+AGG | 0.554622 | 5.3:-14089773 | MS.gene64888:CDS |
| GGTATTATCTGGAGAAGCAA+AGG | 0.556103 | 5.3:-14086837 | MS.gene64888:CDS |
| GGAGGCCATCTTCGTCAGAC+CGG | 0.556644 | 5.3:-14090013 | MS.gene64888:CDS |
| AAACGGAGTTCAGAGGTTCT+GGG | 0.556723 | 5.3:-14088463 | MS.gene64888:CDS |
| AACCTAGAAGTTTGGAAGCA+GGG | 0.559255 | 5.3:+14088757 | None:intergenic |
| ATCAGGTCCTTGCAATACCC+TGG | 0.559752 | 5.3:-14093079 | MS.gene64888:CDS |
| AGCTCAAGTAACAAGTGGCA+TGG | 0.560858 | 5.3:-14085502 | MS.gene64888:CDS |
| TGTTCCTTTAGTCAACTTGG+AGG | 0.562952 | 5.3:-14085566 | MS.gene64888:CDS |
| TTAAGGTGCGATTATGTTTG+GGG | 0.564590 | 5.3:-14085760 | MS.gene64888:intron |
| GTTATGATGTAGAGATTGGT+GGG | 0.564818 | 5.3:-14089730 | MS.gene64888:CDS |
| TTTGGAGTAAGCAAAACCTA+GGG | 0.566038 | 5.3:-14089601 | MS.gene64888:CDS |
| ATGACGCTGCTGAGATTGAA+GGG | 0.566125 | 5.3:-14088426 | MS.gene64888:CDS |
| AATAACCGGTCTGACGAAGA+TGG | 0.566285 | 5.3:+14090008 | None:intergenic |
| GATGATGATAGAAAGGAAGT+AGG | 0.569154 | 5.3:-14094296 | MS.gene64888:CDS |
| TTTGAAGGTTATGAGTGCCA+TGG | 0.569684 | 5.3:-14089758 | MS.gene64888:CDS |
| AGTGCCTCCAAGTTGACTAA+AGG | 0.574131 | 5.3:+14085562 | None:intergenic |
| TTGCAACTTCTACACAGGGA+CGG | 0.575314 | 5.3:+14089543 | None:intergenic |
| GACAACATAACTGGAGTTTG+AGG | 0.579541 | 5.3:+14087882 | None:intergenic |
| GGAGGAAACAAATCCAATCC+AGG | 0.580368 | 5.3:-14093529 | MS.gene64888:intron |
| TTGATTGAAGTACCTTATTG+GGG | 0.583424 | 5.3:+14093381 | None:intergenic |
| CTTCTACTTCTATGAAATGG+AGG | 0.585489 | 5.3:+14088846 | None:intergenic |
| AGGTCCCTTGCAAACGAACT+GGG | 0.587913 | 5.3:+14092781 | None:intergenic |
| AATGACAATCGCTCTTGTTG+CGG | 0.589049 | 5.3:-14086292 | MS.gene64888:intron |
| GAGGTGGAATTACTCGAGAT+GGG | 0.591799 | 5.3:-14095372 | MS.gene64888:CDS |
| TAGAAGTTTGGAAGCAGGGT+CGG | 0.592890 | 5.3:+14088761 | None:intergenic |
| TACTTGAAAGTAGTTGATGA+TGG | 0.593271 | 5.3:-14095655 | MS.gene64888:intron |
| ATTTCTTACTGATAGTATCA+GGG | 0.593444 | 5.3:+14087656 | None:intergenic |
| TTTGTTTCCTCCGAACTGGT+TGG | 0.594493 | 5.3:+14093540 | None:intergenic |
| TTGACTAAAGGAACAGTGGT+TGG | 0.596025 | 5.3:+14085574 | None:intergenic |
| CGAGATGGGCTGGAATTGGT+GGG | 0.596864 | 5.3:-14095358 | MS.gene64888:CDS |
| GAGCATCTAGATGCAGTACA+AGG | 0.602335 | 5.3:-14089214 | MS.gene64888:CDS |
| AACGGAGTTCAGAGGTTCTG+GGG | 0.602590 | 5.3:-14088462 | MS.gene64888:CDS |
| ATAATCTCACCTGGAAACAG+AGG | 0.602946 | 5.3:+14085682 | None:intergenic |
| ACTTCAGTTATGACATGTCA+AGG | 0.604592 | 5.3:-14089154 | MS.gene64888:CDS |
| ATGAATCACCTTCATATCCA+GGG | 0.605531 | 5.3:+14093062 | None:intergenic |
| TTCAAGATATATGCAGGGCA+TGG | 0.607221 | 5.3:+14092659 | None:intergenic |
| TGGAGACGAAAGAATCAGAC+TGG | 0.608130 | 5.3:-14086949 | MS.gene64888:CDS |
| GACCTGATCAATAACAGCAA+GGG | 0.609846 | 5.3:+14087567 | None:intergenic |
| CTCAAAACATATAAAGAGCG+GGG | 0.609938 | 5.3:+14092626 | None:intergenic |
| GGTTTGAGCATCGTAACTCG+TGG | 0.611142 | 5.3:-14092727 | MS.gene64888:CDS |
| TGCCAAAGTTCTTCATCAGG+TGG | 0.611303 | 5.3:-14087623 | MS.gene64888:CDS |
| ACTAGAAGGGAGCATTCTGA+TGG | 0.612665 | 5.3:+14085726 | None:intergenic |
| CTGTTGATTACTTACATCGA+GGG | 0.615245 | 5.3:-14090066 | MS.gene64888:CDS |
| CTGAATACCATGAATCTAGA+GGG | 0.615850 | 5.3:+14089456 | None:intergenic |
| AAGAGTTGCTCTACACAAAG+AGG | 0.616039 | 5.3:-14089686 | MS.gene64888:CDS |
| CGACTTCACAAGAATCGTTG+AGG | 0.618882 | 5.3:-14095839 | MS.gene64888:CDS |
| GGAGTAAGCAAAACCTAGGG+GGG | 0.619378 | 5.3:-14089598 | MS.gene64888:CDS |
| CTACATCATAACCGAACCCA+TGG | 0.620434 | 5.3:+14089741 | None:intergenic |
| GCTGAATACCATGAATCTAG+AGG | 0.621627 | 5.3:+14089455 | None:intergenic |
| TAATCTCACCTGGAAACAGA+GGG | 0.622362 | 5.3:+14085683 | None:intergenic |
| TGTCTGAAGTGGAAAAGAAG+AGG | 0.623855 | 5.3:+14090140 | None:intergenic |
| CAATCAAGAAATGTGTGATG+CGG | 0.624369 | 5.3:-14093361 | MS.gene64888:CDS |
| TTTAGGAGTCGTACAACCGA+GGG | 0.628386 | 5.3:+14085974 | None:intergenic |
| AGCTCAAAACATATAAAGAG+CGG | 0.628689 | 5.3:+14092624 | None:intergenic |
| TCAACTACTTCATAGTTACA+GGG | 0.628877 | 5.3:-14086674 | MS.gene64888:CDS |
| CTAAATTCAAAATCGTGCAG+AGG | 0.632682 | 5.3:-14085956 | MS.gene64888:intron |
| TCATTGTGCAGTGTGCTCAT+GGG | 0.633237 | 5.3:-14085600 | MS.gene64888:intron |
| CATTTCATAGAAGTAGAAGG+AGG | 0.633795 | 5.3:-14088842 | MS.gene64888:CDS |
| TTCATATCCAGGGTATTGCA+AGG | 0.634101 | 5.3:+14093072 | None:intergenic |
| TGGAGTAAGCAAAACCTAGG+GGG | 0.636676 | 5.3:-14089599 | MS.gene64888:CDS |
| CAATGCAGTAGCTCGAGATG+AGG | 0.637844 | 5.3:+14089361 | None:intergenic |
| TAATGTGAAACGGAGTTCAG+AGG | 0.639049 | 5.3:-14088470 | MS.gene64888:CDS |
| ACAAGAGCTCAAGTAACAAG+TGG | 0.645352 | 5.3:-14085507 | MS.gene64888:CDS |
| TGACAATCATCAGTGCTACA+GGG | 0.650522 | 5.3:-14090104 | MS.gene64888:CDS |
| GAGATTATGGGAATAAATGG+AGG | 0.650561 | 5.3:-14087914 | MS.gene64888:CDS |
| AGGGAACAGATTAAAGATTG+GGG | 0.650828 | 5.3:-14086655 | MS.gene64888:CDS |
| ATTTAGGAGTCGTACAACCG+AGG | 0.651899 | 5.3:+14085973 | None:intergenic |
| GACCTCCACCCCAAAGCCAG+TGG | 0.655193 | 5.3:+14093161 | None:intergenic |
| TAATGACAAGAACTTATGGA+CGG | 0.657018 | 5.3:-14094468 | MS.gene64888:CDS |
| TTAAATTCAAGATATATGCA+GGG | 0.659067 | 5.3:+14092654 | None:intergenic |
| AGACCTGATCAATAACAGCA+AGG | 0.659668 | 5.3:+14087566 | None:intergenic |
| GAGTAAGCAAAACCTAGGGG+GGG | 0.660417 | 5.3:-14089597 | MS.gene64888:CDS |
| AGGAGTCGTACAACCGAGGG+TGG | 0.660860 | 5.3:+14085977 | None:intergenic |
| CAACAAAATGATCCATTCAG+GGG | 0.663093 | 5.3:-14088385 | MS.gene64888:intron |
| AAAGAGAAACAAAATAAGGG+AGG | 0.666982 | 5.3:-14090031 | MS.gene64888:CDS |
| CCAGTGGTTACAAGTGACAG+TGG | 0.669527 | 5.3:+14093177 | None:intergenic |
| TCTACAACCAACCAGTTCGG+AGG | 0.673318 | 5.3:-14093547 | MS.gene64888:CDS |
| ACGGAGTTCAGAGGTTCTGG+GGG | 0.673977 | 5.3:-14088461 | MS.gene64888:CDS |
| AGTTGCTCTACACAAAGAGG+TGG | 0.676104 | 5.3:-14089683 | MS.gene64888:CDS |
| CTTCAGCTAGCTGATACTGA+CGG | 0.676315 | 5.3:-14086013 | MS.gene64888:intron |
| CAAGTTGACTAAAGGAACAG+TGG | 0.677062 | 5.3:+14085570 | None:intergenic |
| TGGGAGAGAAGCATAGCTTG+TGG | 0.677167 | 5.3:-14090578 | MS.gene64888:intron |
| AATGCTACGAGTGAAAACTT+CGG | 0.679690 | 5.3:-14094541 | MS.gene64888:CDS |
| TCTGTTGATTACTTACATCG+AGG | 0.679989 | 5.3:-14090067 | MS.gene64888:CDS |
| TCAAAACATATAAAGAGCGG+GGG | 0.683425 | 5.3:+14092627 | None:intergenic |
| TTGGAGTAAGCAAAACCTAG+GGG | 0.687311 | 5.3:-14089600 | MS.gene64888:CDS |
| GAAGAACTTTGGCATCCTCG+AGG | 0.706267 | 5.3:+14087632 | None:intergenic |
| TATGCGAGAAGTTCTTCACG+TGG | 0.708464 | 5.3:-14089824 | MS.gene64888:CDS |
| TCGATGTAAGTAATCAACAG+AGG | 0.724205 | 5.3:+14090069 | None:intergenic |
| CAATCATCAGTGCTACAGGG+AGG | 0.729888 | 5.3:-14090101 | MS.gene64888:CDS |
| CCTTTGTAGCGCGAGCATCG+AGG | 0.736933 | 5.3:+14095800 | None:intergenic |
| ATGGTAGCTAAAAGACCCAG+CGG | 0.738543 | 5.3:-14088792 | MS.gene64888:CDS |
| TTGGGGCTAAGAGCATCAGG+AGG | 0.744656 | 5.3:+14088619 | None:intergenic |
| TATCAGTAAGAAATGCCTCG+AGG | 0.751021 | 5.3:-14087647 | MS.gene64888:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 14085438 | 14095926 | 14085438 | ID=MS.gene64888 |
| chr5.3 | mRNA | 14085438 | 14095926 | 14085438 | ID=MS.gene64888.t1;Parent=MS.gene64888 |
| chr5.3 | exon | 14095801 | 14095926 | 14095801 | ID=MS.gene64888.t1.exon1;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14095801 | 14095926 | 14095801 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14095656 | 14095707 | 14095656 | ID=MS.gene64888.t1.exon2;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14095656 | 14095707 | 14095656 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14095347 | 14095394 | 14095347 | ID=MS.gene64888.t1.exon3;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14095347 | 14095394 | 14095347 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14094441 | 14094595 | 14094441 | ID=MS.gene64888.t1.exon4;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14094441 | 14094595 | 14094441 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14094288 | 14094345 | 14094288 | ID=MS.gene64888.t1.exon5;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14094288 | 14094345 | 14094288 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14093530 | 14093587 | 14093530 | ID=MS.gene64888.t1.exon6;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14093530 | 14093587 | 14093530 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14093311 | 14093403 | 14093311 | ID=MS.gene64888.t1.exon7;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14093311 | 14093403 | 14093311 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14093071 | 14093236 | 14093071 | ID=MS.gene64888.t1.exon8;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14093071 | 14093236 | 14093071 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14092936 | 14092951 | 14092936 | ID=MS.gene64888.t1.exon9;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14092936 | 14092951 | 14092936 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14092592 | 14092812 | 14092592 | ID=MS.gene64888.t1.exon10;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14092592 | 14092812 | 14092592 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14090579 | 14090648 | 14090579 | ID=MS.gene64888.t1.exon11;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14090579 | 14090648 | 14090579 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14090406 | 14090486 | 14090406 | ID=MS.gene64888.t1.exon12;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14090406 | 14090486 | 14090406 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14088760 | 14090205 | 14088760 | ID=MS.gene64888.t1.exon13;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14088760 | 14090205 | 14088760 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14088592 | 14088670 | 14088592 | ID=MS.gene64888.t1.exon14;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14088592 | 14088670 | 14088592 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14088386 | 14088495 | 14088386 | ID=MS.gene64888.t1.exon15;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14088386 | 14088495 | 14088386 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14087893 | 14087966 | 14087893 | ID=MS.gene64888.t1.exon16;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14087893 | 14087966 | 14087893 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14087570 | 14087764 | 14087570 | ID=MS.gene64888.t1.exon17;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14087570 | 14087764 | 14087570 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14086932 | 14086976 | 14086932 | ID=MS.gene64888.t1.exon18;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14086932 | 14086976 | 14086932 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14086805 | 14086858 | 14086805 | ID=MS.gene64888.t1.exon19;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14086805 | 14086858 | 14086805 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14086610 | 14086716 | 14086610 | ID=MS.gene64888.t1.exon20;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14086610 | 14086716 | 14086610 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14086293 | 14086341 | 14086293 | ID=MS.gene64888.t1.exon21;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14086293 | 14086341 | 14086293 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14086130 | 14086189 | 14086130 | ID=MS.gene64888.t1.exon22;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14086130 | 14086189 | 14086130 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14085957 | 14086029 | 14085957 | ID=MS.gene64888.t1.exon23;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14085957 | 14086029 | 14085957 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14085692 | 14085777 | 14085692 | ID=MS.gene64888.t1.exon24;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14085692 | 14085777 | 14085692 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| chr5.3 | exon | 14085438 | 14085611 | 14085438 | ID=MS.gene64888.t1.exon25;Parent=MS.gene64888.t1 |
| chr5.3 | CDS | 14085438 | 14085611 | 14085438 | ID=cds.MS.gene64888.t1;Parent=MS.gene64888.t1 |
| Gene Sequence |
| Protein sequence |
