Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65046.t1 | KEH18365.1 | 73.8 | 61 | 15 | 1 | 15 | 74 | 37 | 97 | 6.50E-12 | 79.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65046.t1 | A0A072TXY0 | 73.8 | 61 | 15 | 1 | 15 | 74 | 37 | 97 | 4.7e-12 | 79.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049786 | MS.gene65046 | 0.80176 | 7.73E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene65046 | 0.804657 | 1.94E-49 | -1.69E-46 |
| MS.gene053381 | MS.gene65046 | 0.802882 | 4.54E-49 | -1.69E-46 |
| MS.gene055603 | MS.gene65046 | 0.806251 | 9.01E-50 | -1.69E-46 |
| MS.gene06010 | MS.gene65046 | 0.80628 | 8.88E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene65046 | 0.802802 | 4.72E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65046.t1 | MTR_8g016500 | 75.862 | 58 | 13 | 1 | 18 | 74 | 40 | 97 | 1.07e-22 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 8 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGAACTATTGTTGTTTGT+TGG | 0.354497 | 7.1:+44256000 | None:intergenic |
| TGGAAATTGCGACACTTAGA+AGG | 0.368870 | 7.1:-44255947 | MS.gene65046:CDS |
| TTGTTGTTTGTTGGATCTGT+TGG | 0.448449 | 7.1:+44256009 | None:intergenic |
| TGCGACACTTAGAAGGACTA+TGG | 0.475465 | 7.1:-44255940 | MS.gene65046:CDS |
| AGAATGTGTCGAGAAGGAAA+TGG | 0.537490 | 7.1:-44255967 | MS.gene65046:CDS |
| ATGTAGAGAATGTGTCGAGA+AGG | 0.597119 | 7.1:-44255973 | MS.gene65046:CDS |
| AGGACTATGGTGAAGATGCA+TGG | 0.669626 | 7.1:-44255927 | MS.gene65046:CDS |
| TAGGCTCGTAGAATTGATTG+TGG | 0.686326 | 7.1:-44256065 | MS.gene65046:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTGAACTATTGTTGTTTGT+TGG | + | chr7.1:44255914-44255933 | None:intergenic | 30.0% |
| !! | TTGTTGTTTGTTGGATCTGT+TGG | + | chr7.1:44255905-44255924 | None:intergenic | 35.0% |
| AGAATGTGTCGAGAAGGAAA+TGG | - | chr7.1:44255944-44255963 | MS.gene65046:CDS | 40.0% | |
| ATGTAGAGAATGTGTCGAGA+AGG | - | chr7.1:44255938-44255957 | MS.gene65046:CDS | 40.0% | |
| TGGAAATTGCGACACTTAGA+AGG | - | chr7.1:44255964-44255983 | MS.gene65046:CDS | 40.0% | |
| AGGACTATGGTGAAGATGCA+TGG | - | chr7.1:44255984-44256003 | MS.gene65046:CDS | 45.0% | |
| TGCGACACTTAGAAGGACTA+TGG | - | chr7.1:44255971-44255990 | MS.gene65046:CDS | 45.0% | |
| !!! | GTGGCTGCAGTTTTTTTGAC+TGG | - | chr7.1:44255865-44255884 | MS.gene65046:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 44255849 | 44256084 | 44255849 | ID=MS.gene65046 |
| chr7.1 | mRNA | 44255849 | 44256084 | 44255849 | ID=MS.gene65046.t1;Parent=MS.gene65046 |
| chr7.1 | exon | 44255849 | 44256084 | 44255849 | ID=MS.gene65046.t1.exon1;Parent=MS.gene65046.t1 |
| chr7.1 | CDS | 44255849 | 44256084 | 44255849 | ID=cds.MS.gene65046.t1;Parent=MS.gene65046.t1 |
| Gene Sequence |
| Protein sequence |
