AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65314

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65314.t1 MTR_5g023960 99.329 596 4 0 1 596 1 596 0.0 1233
MS.gene65314.t1 MTR_5g023960 99.161 596 4 1 1 596 1 595 0.0 1226
MS.gene65314.t1 MTR_7g068890 45.470 585 305 7 10 591 14 587 0.0 533
MS.gene65314.t1 MTR_4g124630 29.254 523 286 18 63 510 96 609 7.28e-44 167
MS.gene65314.t1 MTR_4g124630 29.254 523 286 18 63 510 96 609 8.88e-44 167
MS.gene65314.t1 MTR_4g124630 29.254 523 286 18 63 510 96 609 1.05e-43 167
MS.gene65314.t1 MTR_4g032925 26.157 432 268 14 63 469 113 518 8.74e-31 128
MS.gene65314.t1 MTR_4g032925 35.659 129 81 2 389 516 513 640 1.43e-14 77.4
MS.gene65314.t1 MTR_4g032925 26.157 432 268 14 63 469 113 518 1.39e-30 128
MS.gene65314.t1 MTR_4g032925 35.659 129 81 2 389 516 513 640 1.74e-14 77.4
MS.gene65314.t1 MTR_8g022740 29.373 303 193 8 63 348 58 356 8.50e-29 122
MS.gene65314.t1 MTR_8g022740 38.760 129 77 2 389 516 453 580 1.34e-14 77.8
MS.gene65314.t1 MTR_8g022740 29.373 303 193 8 63 348 102 400 9.14e-29 122
MS.gene65314.t1 MTR_8g022740 38.760 129 77 2 389 516 498 625 1.27e-14 77.8
MS.gene65314.t1 MTR_8g022740 31.004 229 137 8 137 348 4 228 1.31e-17 87.0
MS.gene65314.t1 MTR_8g022740 38.760 129 77 2 389 516 325 452 1.42e-14 77.4
MS.gene65314.t1 MTR_4g032935 35.659 129 81 2 389 516 288 415 1.63e-14 77.4
MS.gene65314.t1 MTR_4g032935 26.335 281 167 9 210 469 32 293 3.66e-14 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65314.t1 AT3G51460 74.958 595 147 1 2 594 1 595 0.0 978
MS.gene65314.t1 AT5G66020 74.915 586 146 1 10 594 6 591 0.0 962
MS.gene65314.t1 AT3G51830 46.037 593 308 6 2 591 1 584 0.0 567
MS.gene65314.t1 AT3G51830 44.660 206 109 3 2 205 1 203 4.54e-53 181
MS.gene65314.t1 AT1G17340 28.235 510 302 16 63 516 90 591 1.60e-47 178
MS.gene65314.t1 AT3G14205 28.244 524 290 16 63 510 90 603 1.84e-43 166
MS.gene65314.t1 AT5G20840 28.327 526 286 18 62 510 92 603 1.17e-40 158
MS.gene65314.t1 AT1G22620 27.022 544 292 17 63 516 105 633 8.55e-40 156
MS.gene65314.t1 AT3G14205 29.398 415 212 14 172 510 19 428 9.42e-31 127
MS.gene65314.t1 AT3G43220 29.508 305 183 12 67 349 89 383 7.71e-25 110
MS.gene65314.t1 AT3G43220 38.583 127 68 3 389 510 477 598 1.07e-12 71.6
MS.gene65314.t1 AT3G43220 29.508 305 183 12 67 349 89 383 1.23e-24 109
MS.gene65314.t1 AT3G43220 38.583 127 68 3 389 510 477 598 1.13e-12 71.6
MS.gene65314.t1 AT3G43220 29.508 305 183 12 67 349 89 383 1.37e-24 109
MS.gene65314.t1 AT3G43220 38.583 127 68 3 389 510 477 598 1.17e-12 71.6

Find 127 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTACACGCGTATGCGATTA+TGG 0.159877 5.2:+14864511 MS.gene65314:CDS
AATTATTCAAATAATATCTT+TGG 0.226950 5.2:-14872103 None:intergenic
CTTAGAAGGCTTGGTGTCTT+TGG 0.229696 5.2:+14871322 MS.gene65314:CDS
CAGTCATTTCCTCTGGCTTT+GGG 0.233834 5.2:+14873342 MS.gene65314:intron
TTGTTCTGTTTCCACAAAAT+TGG 0.237624 5.2:-14868274 None:intergenic
GCAGTCATTTCCTCTGGCTT+TGG 0.249369 5.2:+14873341 MS.gene65314:intron
TGGATCTTCTGGTTCTTCTT+TGG 0.251618 5.2:+14864569 MS.gene65314:CDS
CATTACTTTCAAGCAGCTAT+TGG 0.272665 5.2:+14867690 MS.gene65314:CDS
GTTGGTATTCTAACATATTT+CGG 0.288353 5.2:-14871300 None:intergenic
AATCTAGATTGCTTCCTCTT+TGG 0.290136 5.2:+14866783 MS.gene65314:CDS
GTATACTGCTTCATTTGTTC+AGG 0.310161 5.2:+14868314 MS.gene65314:CDS
TGGACTACAGGGAGTAAGTA+TGG 0.328583 5.2:-14867505 None:intergenic
TGTTGGGATATTGAGGCTTT+TGG 0.330982 5.2:+14864814 MS.gene65314:CDS
TTGGCTGTTAGTCGAGTTGA+TGG 0.339009 5.2:+14864588 MS.gene65314:CDS
GAGCCAACGGATGGATCTTC+TGG 0.342851 5.2:+14864558 MS.gene65314:CDS
ATTTCCTCTGGCTTTGGGTC+TGG 0.342923 5.2:+14873347 MS.gene65314:CDS
ACTCGAACAGTGGTGCATTC+TGG 0.344734 5.2:-14864764 None:intergenic
AAGATTTGAATGCGAACTAA+TGG 0.346601 5.2:-14871356 None:intergenic
AGGCAGAACCTCGATTTCTA+TGG 0.358839 5.2:+14867344 MS.gene65314:intron
TGTTAGAATACCAACTTAGA+AGG 0.376857 5.2:+14871308 MS.gene65314:CDS
GATATAAGTATTCAGTATTC+AGG 0.380073 5.2:+14871557 MS.gene65314:CDS
ATTACTTTCAAGCAGCTATT+GGG 0.393042 5.2:+14867691 MS.gene65314:CDS
CAACTAACCGATGAGTTTCA+TGG 0.397960 5.2:-14864613 None:intergenic
ATGTTTGCAGTCATTTCCTC+TGG 0.406972 5.2:+14873335 MS.gene65314:intron
TTTGGGCACCGCACCGTTCA+AGG 0.413179 5.2:+14872053 MS.gene65314:CDS
GCCCTTAGGATCATATTTGA+TGG 0.414294 5.2:+14865455 MS.gene65314:intron
ATATCTTCTGTTGAATGAAA+AGG 0.416622 5.2:+14871041 MS.gene65314:CDS
TAAAAGTACCCCTGCCGAAC+AGG 0.417048 5.2:+14865569 MS.gene65314:CDS
CGCCCAGGTCATTCAACCGC+TGG 0.421100 5.2:-14866757 None:intergenic
TGAATGTGCTGGATCTTACC+TGG 0.422927 5.2:+14865491 MS.gene65314:CDS
ATTGCTTCCTCTTTGGAGAC+AGG 0.424898 5.2:+14866790 MS.gene65314:CDS
TGCCCAGCGGTTGAATGACC+TGG 0.425182 5.2:+14866754 MS.gene65314:CDS
TGAGATGCAGCCACGAGTCC+TGG 0.428050 5.2:+14870123 MS.gene65314:intron
GGCAGGCATTAGTGTTGGTT+TGG 0.430204 5.2:+14874244 MS.gene65314:CDS
TACTCACAGCTATAGCTTCA+AGG 0.434888 5.2:-14872731 None:intergenic
ATACATGTGTAGAGCATGAT+AGG 0.435367 5.2:+14871277 MS.gene65314:intron
AGGAGCAACTTGGAGTTGTT+AGG 0.436156 5.2:+14871076 MS.gene65314:CDS
TTGATGTCACTTTGATTGCT+AGG 0.436761 5.2:+14867724 MS.gene65314:CDS
TATCTTCTGTTGAATGAAAA+GGG 0.437236 5.2:+14871042 MS.gene65314:CDS
AGCAAATATACCTGTTCGGC+AGG 0.438913 5.2:-14865579 None:intergenic
GAACAACTTATGCAGTTCAA+TGG 0.449301 5.2:+14868291 MS.gene65314:CDS
AGGATACTGCCTTGGACTAC+AGG 0.453559 5.2:-14867517 None:intergenic
GTGGTTCGATTCCTCTGCTC+TGG 0.454149 5.2:+14869968 MS.gene65314:CDS
GTACACGCGTATGCGATTAT+GGG 0.456721 5.2:+14864512 MS.gene65314:CDS
TTTCAAAATTTCATCCATGA+AGG 0.458958 5.2:+14865524 MS.gene65314:CDS
GAATACCAACTTAGAAGGCT+TGG 0.465347 5.2:+14871313 MS.gene65314:CDS
TTGGCAGAAGAAGGCGGAGT+TGG 0.472243 5.2:+14865673 MS.gene65314:intron
ATCAAATAAGGATACTGCCT+TGG 0.476731 5.2:-14867525 None:intergenic
GAACATGTCCACAGATATGA+TGG 0.476890 5.2:-14870617 None:intergenic
GGCTCAATCACATACTGATC+AGG 0.477895 5.2:-14864540 None:intergenic
CAATAGCTGCTTGAAAGTAA+TGG 0.481528 5.2:-14867689 None:intergenic
GCAGCATTTGTGAGGGCCAA+TGG 0.481559 5.2:+14874266 MS.gene65314:CDS
ATTTGTGAGGGCCAATGGAC+GGG 0.481994 5.2:+14874271 MS.gene65314:CDS
CTCTGAAAGGAGACTTTGTC+AGG 0.482455 5.2:+14871588 MS.gene65314:CDS
AACAACTTATGCAGTTCAAT+GGG 0.484304 5.2:+14868292 MS.gene65314:CDS
GAGAAAATGAAGGAGCAACT+TGG 0.489109 5.2:+14871066 MS.gene65314:CDS
AGTTTCAATAACTCAAACTT+CGG 0.494399 5.2:-14870018 None:intergenic
TGATCAGTGTGGGCTAATCA+TGG 0.505915 5.2:+14871530 MS.gene65314:intron
ATAACTGAGCGTGAATGTGC+TGG 0.512069 5.2:+14865480 MS.gene65314:CDS
ACAAAGTCTCCTTTCAGAGC+AGG 0.513920 5.2:-14871584 None:intergenic
ATGTGGCTAGTGACGATGTA+AGG 0.514590 5.2:+14870472 MS.gene65314:CDS
ATCATTTATTATCCCTTGAA+CGG 0.519452 5.2:-14872066 None:intergenic
AAATTGGCAACATATCCATC+TGG 0.521128 5.2:-14868258 None:intergenic
ATGTGGGCAGGCATTAGTGT+TGG 0.524019 5.2:+14874239 MS.gene65314:CDS
CTGTAGCAAATATACCTGTT+CGG 0.524801 5.2:-14865583 None:intergenic
AATGTTGCTGAGAAAACTAC+TGG 0.525748 5.2:+14865713 MS.gene65314:CDS
TTGGTGTTGTTGGGATATTG+AGG 0.526621 5.2:+14864807 MS.gene65314:CDS
CAACCTGAGTTACATTTGTA+CGG 0.533415 5.2:-14871122 None:intergenic
AATCTAATATATGCCTCTCC+AGG 0.534684 5.2:-14870141 None:intergenic
AGACACCAAGCCTTCTAAGT+TGG 0.543822 5.2:-14871318 None:intergenic
TTTGATTTCCATCATATCTG+TGG 0.545603 5.2:+14870609 MS.gene65314:CDS
GAATGTGCTGGATCTTACCT+GGG 0.550082 5.2:+14865492 MS.gene65314:CDS
GCCCAGGTCATTCAACCGCT+GGG 0.555458 5.2:-14866756 None:intergenic
CATTTGTGAGGGCCAATGGA+CGG 0.556420 5.2:+14874270 MS.gene65314:CDS
TGAAAAGGGAGAGAAAATGA+AGG 0.557341 5.2:+14871056 MS.gene65314:CDS
AAGGCCTCCTTTCTGAGAAG+AGG 0.559157 5.2:-14872712 None:intergenic
CAAATATACCTGTTCGGCAG+GGG 0.559670 5.2:-14865577 None:intergenic
GTTGCAGAAAACCCGTCCAT+TGG 0.560740 5.2:-14874282 None:intergenic
TTGGTTTGGCAGCATTTGTG+AGG 0.563885 5.2:+14874258 MS.gene65314:CDS
GAGAAGAGGCAGCTGTATCT+CGG 0.564438 5.2:-14872698 None:intergenic
AGGAGATGCACAAGAAGAAA+CGG 0.565823 5.2:+14867744 MS.gene65314:CDS
GCAAATATACCTGTTCGGCA+GGG 0.569564 5.2:-14865578 None:intergenic
ATCAGCATGGAGGTGAAGGA+CGG 0.571732 5.2:+14870418 MS.gene65314:intron
GTTCAAGGGATAATAAATGA+TGG 0.579780 5.2:+14872068 MS.gene65314:CDS
TTGGGCACCGCACCGTTCAA+GGG 0.580521 5.2:+14872054 MS.gene65314:CDS
GCCCAGCGGTTGAATGACCT+GGG 0.585038 5.2:+14866755 MS.gene65314:CDS
TACTTACTCCCTGTAGTCCA+AGG 0.585221 5.2:+14867508 MS.gene65314:CDS
GCAACCATGTCGTTGAGACA+AGG 0.585817 5.2:+14873390 MS.gene65314:CDS
GGCTGTTGATCTTGTTAACA+AGG 0.589165 5.2:+14870189 MS.gene65314:CDS
TCAGGAACTCCTGCTCTGAA+AGG 0.591042 5.2:+14871575 MS.gene65314:CDS
GGACATTATATAGTTTCTGT+CGG 0.591289 5.2:+14872675 MS.gene65314:CDS
GCAATCTAGATTCATCGCCC+AGG 0.594749 5.2:-14866772 None:intergenic
TATGTGATTGAGCCAACGGA+TGG 0.596228 5.2:+14864549 MS.gene65314:CDS
ACAGCTGCCTCTTCTCAGAA+AGG 0.596485 5.2:+14872705 MS.gene65314:CDS
GGAACTCGAATGTGGAGAAG+AGG 0.596581 5.2:+14868228 MS.gene65314:intron
TGGTTTGGCAGCATTTGTGA+GGG 0.597710 5.2:+14874259 MS.gene65314:CDS
AGAAGAGGAGCTGATCCAGA+TGG 0.598685 5.2:+14868243 MS.gene65314:CDS
GAACCAGAAGATCCATCCGT+TGG 0.601871 5.2:-14864561 None:intergenic
GTCTAGACAATCAATACAGT+TGG 0.605671 5.2:-14871100 None:intergenic
GGACTACAGGGAGTAAGTAT+GGG 0.606764 5.2:-14867504 None:intergenic
GATCAGTGTGGGCTAATCAT+GGG 0.607706 5.2:+14871531 MS.gene65314:intron
GATGGATCCATGAAACTCAT+CGG 0.608786 5.2:+14864606 MS.gene65314:CDS
CGTGAGGGATTGTGTAGACG+TGG 0.612699 5.2:-14874308 None:intergenic
ATATGCCTCTCCAGGACTCG+TGG 0.612882 5.2:-14870133 None:intergenic
GGATACTGCCTTGGACTACA+GGG 0.615138 5.2:-14867516 None:intergenic
AAAACCAGACCCAAAGCCAG+AGG 0.617068 5.2:-14873351 None:intergenic
GTAGAAGTTAACTTAACGTG+AGG 0.619548 5.2:-14874324 None:intergenic
AGAAGTGCTCATAGATAACA+AGG 0.620907 5.2:+14867381 MS.gene65314:CDS
AGACCGTACAAATGTAACTC+AGG 0.622330 5.2:+14871119 MS.gene65314:CDS
TGCAGCCACGAGTCCTGGAG+AGG 0.625318 5.2:+14870128 MS.gene65314:intron
GTTTGAGTTATTGAAACTCG+AGG 0.628328 5.2:+14870023 MS.gene65314:CDS
CTCACCTTGTCTCAACGACA+TGG 0.630377 5.2:-14873394 None:intergenic
TAGAAGTTAACTTAACGTGA+GGG 0.633337 5.2:-14874323 None:intergenic
AATCTTCGGAACTCGAACAG+TGG 0.633576 5.2:-14864774 None:intergenic
ACAATTTGCTGCCAGAGCAG+AGG 0.638573 5.2:-14869979 None:intergenic
CCTTGAAAGGAACTCGAATG+TGG 0.638990 5.2:+14868220 MS.gene65314:intron
TGGCAGTACAATGCAGCATG+TGG 0.641511 5.2:+14870455 MS.gene65314:CDS
TAATTGTTCCATAGAAATCG+AGG 0.643849 5.2:-14867352 None:intergenic
ATGAAACTAATTTAACTCTG+AGG 0.647407 5.2:+14865750 MS.gene65314:CDS
TCAGTATGTGATTGAGCCAA+CGG 0.650515 5.2:+14864545 MS.gene65314:CDS
GATGCAATTGATCTTCTGCA+AGG 0.651722 5.2:+14872654 MS.gene65314:CDS
CCATATTTGGCAGAAGAAGG+CGG 0.653074 5.2:+14865667 MS.gene65314:intron
ATAGATACCTGTCTCCAAAG+AGG 0.664244 5.2:-14866797 None:intergenic
GCTGCCTCTTCTCAGAAAGG+AGG 0.666260 5.2:+14872708 MS.gene65314:CDS
ATCAGTGTGGGCTAATCATG+GGG 0.668810 5.2:+14871532 MS.gene65314:intron
TTATTATCCCTTGAACGGTG+CGG 0.669092 5.2:-14872061 None:intergenic
AATGTGCTGGATCTTACCTG+GGG 0.707265 5.2:+14865493 MS.gene65314:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.2 gene 14864480 14874334 14864480 ID=MS.gene65314
chr5.2 mRNA 14864480 14874334 14864480 ID=MS.gene65314.t1;Parent=MS.gene65314
chr5.2 exon 14864480 14864627 14864480 ID=MS.gene65314.t1.exon1;Parent=MS.gene65314.t1
chr5.2 CDS 14864480 14864627 14864480 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14864756 14864839 14864756 ID=MS.gene65314.t1.exon2;Parent=MS.gene65314.t1
chr5.2 CDS 14864756 14864839 14864756 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14865463 14865590 14865463 ID=MS.gene65314.t1.exon3;Parent=MS.gene65314.t1
chr5.2 CDS 14865463 14865590 14865463 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14865680 14865771 14865680 ID=MS.gene65314.t1.exon4;Parent=MS.gene65314.t1
chr5.2 CDS 14865680 14865771 14865680 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14866754 14866811 14866754 ID=MS.gene65314.t1.exon5;Parent=MS.gene65314.t1
chr5.2 CDS 14866754 14866811 14866754 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14867346 14867402 14867346 ID=MS.gene65314.t1.exon6;Parent=MS.gene65314.t1
chr5.2 CDS 14867346 14867402 14867346 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14867499 14867532 14867499 ID=MS.gene65314.t1.exon7;Parent=MS.gene65314.t1
chr5.2 CDS 14867499 14867532 14867499 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14867685 14867765 14867685 ID=MS.gene65314.t1.exon8;Parent=MS.gene65314.t1
chr5.2 CDS 14867685 14867765 14867685 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14868229 14868335 14868229 ID=MS.gene65314.t1.exon9;Parent=MS.gene65314.t1
chr5.2 CDS 14868229 14868335 14868229 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14869964 14870050 14869964 ID=MS.gene65314.t1.exon10;Parent=MS.gene65314.t1
chr5.2 CDS 14869964 14870050 14869964 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14870133 14870210 14870133 ID=MS.gene65314.t1.exon11;Parent=MS.gene65314.t1
chr5.2 CDS 14870133 14870210 14870133 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14870423 14870493 14870423 ID=MS.gene65314.t1.exon12;Parent=MS.gene65314.t1
chr5.2 CDS 14870423 14870493 14870423 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14870599 14870697 14870599 ID=MS.gene65314.t1.exon13;Parent=MS.gene65314.t1
chr5.2 CDS 14870599 14870697 14870599 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14871041 14871140 14871041 ID=MS.gene65314.t1.exon14;Parent=MS.gene65314.t1
chr5.2 CDS 14871041 14871140 14871041 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14871289 14871397 14871289 ID=MS.gene65314.t1.exon15;Parent=MS.gene65314.t1
chr5.2 CDS 14871289 14871397 14871289 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14871537 14871609 14871537 ID=MS.gene65314.t1.exon16;Parent=MS.gene65314.t1
chr5.2 CDS 14871537 14871609 14871537 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14872052 14872145 14872052 ID=MS.gene65314.t1.exon17;Parent=MS.gene65314.t1
chr5.2 CDS 14872052 14872145 14872052 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14872654 14872746 14872654 ID=MS.gene65314.t1.exon18;Parent=MS.gene65314.t1
chr5.2 CDS 14872654 14872746 14872654 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14873345 14873411 14873345 ID=MS.gene65314.t1.exon19;Parent=MS.gene65314.t1
chr5.2 CDS 14873345 14873411 14873345 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
chr5.2 exon 14874204 14874334 14874204 ID=MS.gene65314.t1.exon20;Parent=MS.gene65314.t1
chr5.2 CDS 14874204 14874334 14874204 ID=cds.MS.gene65314.t1;Parent=MS.gene65314.t1
Gene Sequence

>MS.gene65314

ATGATGGAGAGAGCAGATTCTGTGCAGAAGCTGTACACGCGTATGCGATTATGGGAATTTCCTGATCAGTATGTGATTGAGCCAACGGATGGATCTTCTGGTTCTTCTTTGGCTGTTAGTCGAGTTGATGGATCCATGAAACTCATCGGTTAGTTGATTCTATTGATTTGTCTTCTTCTAGTGCTTTTTATGTAATTCATCTACAAAGGATCCATCGATCATTTGAATTTTTTTTCGTTTTATATATTTTGATCTTGGGTTGTTTTGCTTTGACAGATGAGGTTCCAGAATGCACCACTGTTCGAGTTCCGAAGATTTATACGATTTTTGGTGTTGTTGGGATATTGAGGCTTTTGGCTGGTGAGCAATCTTAAGAGAGACATGGCCGTGTTTTGCTTTAATGAAATTTTACATGAAGTGGCTACTGTATGATTGTGTTGCTCTTTAAGTTTTAATTTCCTGTTTATCGGCAAGGTCGATGTTCATCTTGATCTATATGATTGTGTAACAATGTGAGATGATTAGATTACACATTTTTTATTTTTTAGTTGTGTGGGCGGCGTGATTGAAGTTAGCAATTGGGATGTTCGACTAATGATAAATGGTCATTGATTTTGGCACACAAAAGCAGAAATGAATAAAATATCAATGATGTTTGGTGCCATATGGGCTAAGGAAAACAAAGTGTTTTTTTATTTCTTTATTTATTTTTGTAAGAACTGGCACATGGTTTAATGATCATGCTAGCTTTTTGCATTTATATAGACTTCTTTTAGTTCATTTATGTTTATGGCTTAAAAAGCAGGTCCTTACTACCCTGTTTATTAATTGCTTACAATAGTGATTTTGATATTGGTATCCTATTTTCTCGATCTTGTTTACATAATTGATTTCCTTTTAAATACCAGATAATACATTATTTGAAAAGCATAGTTCAATTTGGGCAATTATTTTGATGTTGCTAAGTTTTACTATGCCCTTAGGATCATATTTGATGGTCATAACTGAGCGTGAATGTGCTGGATCTTACCTGGGGCATCCAATTTTCAAAATTTCATCCATGAAGGTTTTTCCATGTGATCATTCACTTAAAAGTACCCCTGCCGAACAGGTATATTTGCTACAGCTCCACTTGTTTCCTATTTGCTTCCATGTATACTTCCAATTTGGAACTCATAATTGCTGCACCATATTTGGCAGAAGAAGGCGGAGTTGGAATTTTCTGGGCTACTAAATGTTGCTGAGAAAACTACTGGTCTGTTTTTCTCATATGAAACTAATTTAACTCTGAGGTGAACTTCTTTCCATATTCCTAGTAGTCTTTCATTTTTATGTCTCTAGAATATGTTATGGTTCTATTTGGATTCTAAAATTCACTGCCTTACTTATTTACATACATGTATGAATATACAGAGAAGAAACTACCCATACATTCTCAGTTTAATCGGTAGGTGCAATCATGCAGCCTGTAAACCTACATAAAATGCAGTTCCCACCTCCCTATTTATGTATTGTAGTGTGTAGAACTTTTAGCATGGCACCTAGGATGACATTACTTCACTTTGACATTTATGTCCAGAGTATCAATAATATATGTTTGAGGGCTTCTGCAAAATAATCATTCATACTACATGTTGAAAGATATCATAAATCATTGCATAGGAAGGGGTGAATATGAGATACTGTACATTTTGCCATTGGTTGCAAACCTCTACGCAGCTGATATCTAGGATAGGAACTTATTTTGATATACAGTTCTATTGATTTTTTTTTTTTTAAAATGAATCTGAAGAAGAAGAACTTCTGCAATCTGACTTTTTTAGAGCTGCAGGTCATTGTAACCTTGGTTAAATTAAAAGACATTAGTTTAGTGATAATAGTGACATTAATATTATCTTAGAAAAGTTGGCTTATTTTTTTATCCTGAATGAAAATTGAGTGCATGCTACGTAAATAAAGCATTACCATGTAAATGTTCCTTCTAGCAAAAATAGTTATCACATATATGAATTAAATCCTATAAATAATGCTTTATGTTGCCTTACCATTCCAAGACAAAATTATTTTGGTAAGATTTTTAATAAGACAAGATTAGATCACCATCAATCTATCTATCAATAAAAAACCCTGTACAAAAAATATTTGTAATATACTATCACTTTTTTTTTGGGAGTAGGATATTTACAGCCCCCCATAAAGCACATAATTTATGGAGGGTTAAAATAAAATATAAATTTGTATTTATTAATACAATAAAATTATCATGTTTTGCAGTGCCCAGCGGTTGAATGACCTGGGCGATGAATCTAGATTGCTTCCTCTTTGGAGACAGGTATCTATTGCCTTTTGTTTTGCCACTGCCCTCCTAGTTTAGAGATTTTCTTTTATTGTGTTAAACCATGTTAGTTGAAAGAAAGTAAAGGGAAGCATATAGGAAAGAGAGAGGAAATGATAAACCAAAGAGAGAGGAATACAAGACTCTCTGTTGTGTGAAACTGATGAAATAATTCACTGAAACATTCAGTAACAGTTAACATATGGGGAAGTCGAGCCTTGAGAAGCTCAATGTAACTAAATCATAATATGTTGATTCAGCATATTTTGTGTTAACACAATTATTAGCCACCTATGGCAATAACATAAACATACTCTTATACTAATATTTATAGAATATTGCCGTTACACTGATATACCCACATGTGCTGACTCTGGTAGAGAGAAAAGAATCACAGTTTCTTCTTGATACCATTCCTGAGCGACACTCCTCATGTTTAGCAGATTATGTCCACTCCGAATTGATTCCTTCTGTAGTTTTTGATTTTCCCCTGCTCTTATTCTGCTTATGCTACCAAATTTTTGATCACAGGCAGAACCTCGATTTCTATGGAACAATTATATGTTAGAAGTGCTCATAGATAACAAGGTAGGTTTATGGTAATTTTTTCACTTGTTCAATTTCTTCATGACTTCCACATTTTTATAGTACTAACTAATAAATTTGTGATTCTCTGCTTGCCAGCTTGACCCATACTTACTCCCTGTAGTCCAAGGCAGTATCCTTATTTGATCATTTCAATGATAGATCATCATTTATGGATTTTCTTATAACTAGTGCATACTTTTGGGGTTCGTATAAGTTTTTGTGTTTATGTTTTGTGAGCACAGAACCATTTCTTTTTCAATGTTATCCTTAGCAAGATATCAGGTTTCCATTACTTTCAAGCAGCTATTGGGAAAGATATTATTGATGTCACTTTGATTGCTAGGAGATGCACAAGAAGAAACGGTATTTTAAACTTTTGGATGAACATATATTTATTTCTGTCCAACCAAGAAAATCTTGTTTCTAGCTTGACTGTGCACTTACAGATATCATGTGTTTCTTACCATTCAAAGAATCAATATCGTGTAGGCCATGAACTTAGTGAAATTAATGCGGTGATATTGCAAGTAATCATCGAAGGTTTCCGAAGGTTTTTACATTACCTTGTAGTTGTCTGTTAGGAGCATATACAGATCTAAGTTACACCTTAAGAAAAAGATATTAAAAGATATATTGTTTCCTTTGTTTATGTTAGGGTTTGAATTTGTCATGTTTGAGGTTTAAAATTATTTTTGGAATATATAATTTGAAGTAATTTTGTATGAGAGAATATGGGGTTGAAATTTATGACTGCTTAAAGAAAGCAATCATAATCTTTTACTGATTGGTTGGCTGATAGTACAAATGAAATTTTATTCCTTGAAAGGAACTCGAATGTGGAGAAGAGGAGCTGATCCAGATGGATATGTTGCCAATTTTGTGGAAACAGAACAACTTATGCAGTTCAATGGGTATACTGCTTCATTTGTTCAGGTATTACTTTCTACGACCACAAATCGATTATTCTAAGAATTAAAATGATGGGCTTGTCATGCAAGATATGTGTGTTCTGTTGACTTAAATCAGAGCAGCAGTCCTAATCATCCTAAAGAGTTGTCATTATTTTGAAATAAGATGGACATTATCTTGTACACTTATAAGCAACTGCATAATTAATGAACCAATGATATTTAACTAGATTATTTTTCTTAGAAGTCGGAAAGTTGATTTCAGTTTTTTATGTCTAATATATTTAAAAAGTTCTTAATTGTCTATACCTATTTGCATTTGGTATTTTTGGACCGTCCAATTAACGGTGTTGAACTTTCAAGAAGCCAGATTTTTGGGGGCTGACAAATGACGAACTAAGAAGTGTTTAATATTTTGTAAATGATACTTTGATATCCTTCCAATTGTATATACGTATTTAGGCTCTTTTTGGTGATTGTGTGACCAGGAACTAGAAGAGAGAGACTATAGGAAGTGAGAAACTAATATACTTTGGTAGTAGTAGGCATGGAAGTAATATGAATATTAGTTGCCATTTTCATCTTGCTATTAGCAAATTATTCAAAAATAAACTTCATGTTTAATGATGCTGGTGCAAAAGCAGTGTCACAGTCTCACAGACAGAAAAGAACATTTTTGTTTCAGTGCCAATAATACCCGGAGCAGCTTTCCATATAACGATACAGAAAGCAATTGCCTTTTCTTTTCAACTCTATTTTTCTGTCACTTGATTTTTTCATTGGGAAATAATTGTTTTAATTCTACCTACTTTCTCTTGATCTCTTGTGTGCAACTTACTATATAACTACCTGGAAGTTATCCTATCTCTTTTTATCTGCTATTCCATGTTTGTTTAAATACAAACTCTACCAAACATGACCGTTGATCCTTGCTAGATTTTGATACTGGCATCATTAACTGTTCAAATTTATATTAGCTTTCTGGTTGGGAAAAATACCAAAACTAAATTCCTGACTAAAATCTTTTGCATTTAGTAAAAAAATTATATTTTGCTACAAATCATCATATTTTGTTCATTCCATTATTTTTTGTTATATTAAGTCACTTTTATTATTGTTAATTTGATTTTCCAGTTTCAAGATGGGTTGATGTTGAACTGAAAAGGTTACTCTTATACTTCTAAGCCAAAGTATTCTCACATGCAATATTGGTAGCTTTAAGGTTAATACAAAAATAGCTTAACCTTCAACTATTTTGGGTATTTTTTTTTTTTTTTTTTTTTTTAATTTTGAGACATGGACCCTCTGATAAGATTTGTTGTACCTATTAGGATATAGGACTTCTATATTAAAAAAATGCTGAGCTGGACTCTTATTTGGTTTGAATGTAGCAATACTAATAGTGATAGTTGAATGTAAACTAGTATTTCCAGGTTTTTCGTGGATATCACTACAACTCTCTTATATTATAGCGAAAATAGGGGATTCTTTCTATTATATGATTTAAAAATCAAGCCAAACAAGGAGGGCTAGGATGCTCAAATTCTGATTTTTGGGATGAATGTTAACTGTGTTTGTACCAAAATCTAGTGTCAATTAAACTTTTATTTCATTTCGTATCAATTAGAAAACATCATGGTTGAGCTTTTGCAGATTCGTGGTTCGATTCCTCTGCTCTGGCAGCAAATTGTTGACTTAACATATAAACCGAAGTTTGAGTTATTGAAACTCGAGGAAGCTGTAAGTGTTTGTTGATTGTTGTATTTCTGTGGTTTTTACATATTTTGTGTATTTCATTTCCATTTCATTGCTTGAGATGCAGCCACGAGTCCTGGAGAGGCATATATTAGATTTGAGAAAAAAATATGGAGCAGTGTTGGCTGTTGATCTTGTTAACAAGGTAAATACACTTCCATTGTATTACAAACTGAGAATGCTTGTATATTGGTTGGGGATTTTACTAAATGATACTAGTCTATGTAATTGCATAGTAAATTTCATTGACAAGTGACAACTGTAAAGAATGCATGCAAACTTAAAATGTGCATGTAATTGTTATGTATCGGCATGCATCTATTTGAATGGTCTTCTTTCTCCTTTGTATTTTATCAGCATGGAGGTGAAGGACGGCTATGTGAAAAATTTGGCAGTACAATGCAGCATGTGGCTAGTGACGATGTAAGGTAGAAGTCTCTTTCAATTAACTTTTAGATTGATTATATTGATATGCATGGATTCTTTTCAGTTTTTTAGGGGATTGCTCATGTGTGCTTAATTATTCTTTGCAGATACGTTCACTTTGATTTCCATCATATCTGTGGACATGTTCATTTTGAACGTCTATCATTGCTTTACGATCAAATATCAGATTTTCTTGAGAGAAATGGGTATGAAATTTTTTTGCTTATGCTATTCGATATCTACTTTCTTGATAATTGTTTAGTAGTTTGAAACATTGACATTGATTGGTGTCATTGAAGCAGGGAGCTATTTTTAAACTACTGATTTGCTAACAAATTCTAACAAATTATATTACTATGTTTATGTTGCTTAATTTGGACCTATCTGCTATATTATCATGGACAATTTTCCAGCACGAAGTCATGATCTGTCTTATAAAAAAAATTCACCTGATTATCCTGTTTTATAATAAAATTTTGCGTTAACAAAGCGCTTCTTTAAAAAAATAAATATATTGACCCGAGTTGCTATTCTGTTATGCAATTGTAGATATCTTCTGTTGAATGAAAAGGGAGAGAAAATGAAGGAGCAACTTGGAGTTGTTAGGACCAACTGTATTGATTGTCTAGACCGTACAAATGTAACTCAGGTTGGTTGGTTTCTATTATGGAAGTGTTCCGTGCCATCCCGAATTAATGGAAAATATTTCTTTGTCCCTCTATAAGGAACACTTTCTTTTGTTTTTATCTGTCCTGGTCATCATTTTTCAGCTTACTAATGTTAAAATACATGTGTAGAGCATGATAGGCCGAAATATGTTAGAATACCAACTTAGAAGGCTTGGTGTCTTTGGTGCTGAAGAAACCATTAGTTCGCATTCAAATCTTGATGAACGCTTTAAGATTTGTAAGTTTATTATTACTTTTCAGATTATGTGATTTGAAATATAGATATATATACGTTGTAATATGTAGTTATTTTCAAGTTTTCATTGTTTGTCTCTCTTGAAACCTTTGATTCAATTTGTAACTGATTCTGTGATCAGTGTGGGCTAATCATGGGGACGATATAAGTATTCAGTATTCAGGAACTCCTGCTCTGAAAGGAGACTTTGTCAGGTATTTTCCCATTAGTCTTTACAAAAAATTTCATGTTCCTTGCATATTAAGAGGATCATGAAATTATGTGTCCATAAAAGCTTTTCTATGTTCAATTTACATTAGTATGAAATGATTTTGAACCATCATTGCTGCTCTCATATGATATGCTGACTCTCACTGTATATGTTTTCTAGTACGGGAATTGTGATTTATTTATTTATTTATTTCCATATTAGTCTGTTGCTTTCAATTAGAATTACAAAAACTGGCAGCTGGCAATAAGAAAAACAGTAATGTGATTGTGCAATTCTTTTATATGTTATGGTTGAATAAATTCCATGTGACAATTTGATAAGATCTCTGCGTATAAAGTTTCAAATATGTGTTGTTATTGCGAATTATAAGATTTGAAATTTAACCTTTTGTGACTTATCTCGTTGATTTTTTTGGTGGGATTCAGGTTTGGGCACCGCACCGTTCAAGGGATAATAAATGATGGTTGCAATGCTCTCCAAAGATATTATTTGAATAATTTTGTTGATGGAACAAAGCAGGTTTGTAGAACTTACTTAACATAATATATGAGGAAAAACCAAGTCCCACGTAGAATAAAGATAAGGCATGAAATGACTTTATAAAAGAGTGACACTCACATTATAAGCCGGTTTTGTGAGGATGCCTTACACCCAATATAAAAATCTAATAATATGTGTGGGGTACTCTAGTTATGGTCTCTAGAGTTCCTTAGGTGTCTACTAGTTGCCTATTCTCAACTTATTTTCATCTTGGTTTTGTCCTTTGTATATTTGATTTCTCCCTTCTGCATCCTAGTCTGTGCTACCCTACATGGTAATTATGCTGCAGAAGATGCCAATTTTTCATGTGCATATTTCAATGCTGATTACTCTTATTTCTTATGATACCCTGTCTGAAATGATCTCGAGTTAAATTACTTTTAAGCTTTCTGGATCTATTACCATTTTTAAATTTGTTAATTAATATCTTATTAATTTATATGTAATTGATGATTTATATTGTTCATATTTGTTTTATTATGTGAAGGATGCAATTGATCTTCTGCAAGGACATTATATAGTTTCTGTCGGCCGAGATACAGCTGCCTCTTCTCAGAAAGGAGGCCTTGAAGCTATAGCTGTGAGTACAACATCGATGAAATTTTTTAATTGATGTTTGTGGCTTCAATTTTGTGTTTAAGATCGGTTCTTAAAATGCTCGCACAATTTATAAACATTTTAATTTCTATGATGTACGGCTAAATTCATAGTGGTATACTAAATATGAGATGATTGAATTGAAATGTATTTTATGTGGAAATTTCCTATATCTCACAGAAAAAAATAATTAAACGAAAACTTACTATTTTCTCTCTTTTATTGTGTTTTGAGAGACATGTGTTCCTTTCAAGGTTTACATAAAGCTGGTTGTAACCCTACTGAAAAGTGGTGGGATGCAATGGGCCTCATGAAGCCAAGTGGTTTAAGCTTTATTTAATAAGAAATAAAAACTGCCATTGATAGTTAAAAGAGCAACCAGATTATTCAGTCAAGGAAATGTTAAAGTGACTATATTGCTGGATAATGCATTACATCATTGCCGTTATGTTTGAAGACCTAATATGCTTTTTATTTTGCATTCTGCACTTTGCTGATATGTAGTTCACTAATAGAATTCTTCAAGCAAGTCATATGCATTTAATGGAAATAAATGTTGATATTATTGTTTATGTTTGCAGTCATTTCCTCTGGCTTTGGGTCTGGTTTTTACTGGATTTCTTTTTGCAACCATGTCGTTGAGACAAGGTGAGTTTAATTTATTTAGTTTCAAGGTTTAAACTAAAAATTAATTGGTCACTCATATGTGCACACATTCGTGCATGGGTATCTCTCTCTGTGTTGGTTTAATAAAAAAATATAGAATCAGCTTACTACTTTAAAGGCGTGTGGTCTAAAATCCAGTGTGACCTTTTCAGAATCAGTTGGGGCAATAAGTTTAGACAGACAATATAGTGGTGGTAGAAGACAATCAAGTGAAATGGAGAATGGGGGACTAGGGGAACCACTGAAGTTTTTTATGGCATGTTAACTTTGAGAACTTGCTTTCTGTTTGTTTTTTTTTACAAGCAGTGAAAACAATTTGCTTTCACCTTACAATCTTTTGAAAATAGAAACAAACCACAAATAAGATGAAATTTTGTTATTAAAAGTGATCAAAAAATGAAAAAATATGTTTCCTCAAACCAAACAGATCCTAAGAGGGATATCTTGTAGCCTACTGCTGCCAAATGTGCATGCCCAGGGTTTCTAGATATTTAAACTTTAAAGCAAAAATGTGTGATGTGAAAGTTTACGCGAATTTATGTGCTTAACTCTTCACAAGCATACAAGCTCACAGTAAAAAATATTGCATATGCCTGTACTTGGTTACATCATTACAAAGTGAAATTTTGTCTGTAGTCTTTAACTGGATAATTATGCGGACTTTGCTAGGGTAGAACAATGACTCTGCACTAGTGATAGGTTAATCAACTATGCATTACAGATATTGTGAAATCTATGGCCTTAAAAGTGTTATTTTGTTTATTGTTGCTGCAGTTCGATATGATTTTCGGCACTTCTTCTTTTCGCTAATGTGGGCAGGCATTAGTGTTGGTTTGGCAGCATTTGTGAGGGCCAATGGACGGGTTTTCTGCAACAGACCACGTCTACACAATCCCTCACGTTAA
Protein sequence

>MS.gene65314.t1

MMERADSVQKLYTRMRLWEFPDQYVIEPTDGSSGSSLAVSRVDGSMKLIDEVPECTTVRVPKIYTIFGVVGILRLLAGSYLMVITERECAGSYLGHPIFKISSMKVFPCDHSLKSTPAEQKKAELEFSGLLNVAEKTTGLFFSYETNLTLSAQRLNDLGDESRLLPLWRQAEPRFLWNNYMLEVLIDNKLDPYLLPVVQGSFHYFQAAIGKDIIDVTLIARRCTRRNGTRMWRRGADPDGYVANFVETEQLMQFNGYTASFVQIRGSIPLLWQQIVDLTYKPKFELLKLEEAPRVLERHILDLRKKYGAVLAVDLVNKHGGEGRLCEKFGSTMQHVASDDVRYVHFDFHHICGHVHFERLSLLYDQISDFLERNGYLLLNEKGEKMKEQLGVVRTNCIDCLDRTNVTQSMIGRNMLEYQLRRLGVFGAEETISSHSNLDERFKILWANHGDDISIQYSGTPALKGDFVRFGHRTVQGIINDGCNALQRYYLNNFVDGTKQDAIDLLQGHYIVSVGRDTAASSQKGGLEAIASFPLALGLVFTGFLFATMSLRQVRYDFRHFFFSLMWAGISVGLAAFVRANGRVFCNRPRLHNPSR