Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66111.t1 | XP_003622912.1 | 86 | 121 | 15 | 1 | 7 | 125 | 56 | 176 | 6.30E-45 | 190.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66111.t1 | G7L174 | 86.0 | 121 | 15 | 1 | 7 | 125 | 56 | 176 | 4.5e-45 | 190.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053449 | MS.gene66111 | 0.807084 | 6.01E-50 | -1.69E-46 |
| MS.gene053813 | MS.gene66111 | 0.800716 | 1.26E-48 | -1.69E-46 |
| MS.gene05592 | MS.gene66111 | 0.813235 | 2.84E-51 | -1.69E-46 |
| MS.gene055941 | MS.gene66111 | 0.806505 | 7.96E-50 | -1.69E-46 |
| MS.gene060193 | MS.gene66111 | 0.821902 | 3.15E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66111.t1 | MTR_7g057180 | 81.818 | 132 | 21 | 2 | 7 | 135 | 56 | 187 | 1.25e-56 | 181 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCTCCAACAGCCTATTCT+AGG | 0.297884 | 7.4:-45547624 | MS.gene66111:CDS |
| CAAGACAGAGATCCCGTTCC+TGG | 0.337367 | 7.4:-45547697 | MS.gene66111:CDS |
| TGTCATTTGTTTCAACAGTT+TGG | 0.337567 | 7.4:-45547447 | MS.gene66111:CDS |
| TTACGAGGCGTTAAAATCTA+AGG | 0.373703 | 7.4:-45547778 | MS.gene66111:CDS |
| GCAGTTGCTCCTGTTGCTGC+TGG | 0.381855 | 7.4:-45547555 | MS.gene66111:CDS |
| AAAGTAATAGAAGAGGAAGA+AGG | 0.434316 | 7.4:+45547473 | None:intergenic |
| AAGTAATAGAAGAGGAAGAA+GGG | 0.449260 | 7.4:+45547474 | None:intergenic |
| GCTATGATTGCGATTGCGTT+AGG | 0.449294 | 7.4:-45547675 | MS.gene66111:CDS |
| AAAGTGAAACCAGCAGCAAC+AGG | 0.472516 | 7.4:+45547546 | None:intergenic |
| TCGCAATCATAGCCAGGAAC+GGG | 0.475891 | 7.4:+45547685 | None:intergenic |
| TAGAATAGGCTGTTGGAGAA+TGG | 0.496417 | 7.4:+45547626 | None:intergenic |
| ATAATTCCGCAATGTGCACC+AGG | 0.498074 | 7.4:-45547501 | MS.gene66111:CDS |
| GACAAAGAAAGTAATAGAAG+AGG | 0.502911 | 7.4:+45547466 | None:intergenic |
| CCAGACGGTGATCCTAGAAT+AGG | 0.519118 | 7.4:+45547612 | None:intergenic |
| AACAGGAGCAACTGCTGAAG+AGG | 0.528098 | 7.4:+45547563 | None:intergenic |
| ATGCTCTAAACATGAGGGTG+AGG | 0.546750 | 7.4:-45547805 | MS.gene66111:CDS |
| ACGCAATCGCAATCATAGCC+AGG | 0.561603 | 7.4:+45547679 | None:intergenic |
| ATTCTGCTTCTGTAATGCAA+AGG | 0.563407 | 7.4:+45547831 | None:intergenic |
| GTGATCCTAGAATAGGCTGT+TGG | 0.567693 | 7.4:+45547619 | None:intergenic |
| AGAATTGAGGTGCAAACAGA+TGG | 0.569655 | 7.4:-45547735 | MS.gene66111:CDS |
| GGTCTGCAGATTCTGTCAGA+AGG | 0.581253 | 7.4:-45547591 | MS.gene66111:CDS |
| ATCGCAATCATAGCCAGGAA+CGG | 0.582402 | 7.4:+45547684 | None:intergenic |
| GTGCAAACAGATGGTAAAGA+GGG | 0.592815 | 7.4:-45547726 | MS.gene66111:CDS |
| AATTAAATGCTCTAAACATG+AGG | 0.593823 | 7.4:-45547811 | MS.gene66111:CDS |
| CCTATTCTAGGATCACCGTC+TGG | 0.596293 | 7.4:-45547612 | MS.gene66111:CDS |
| GGTGCAAACAGATGGTAAAG+AGG | 0.598186 | 7.4:-45547727 | MS.gene66111:CDS |
| ATTAAATGCTCTAAACATGA+GGG | 0.602499 | 7.4:-45547810 | MS.gene66111:CDS |
| GACAGAATCTGCAGACCAGA+CGG | 0.612560 | 7.4:+45547597 | None:intergenic |
| GGATGCTATTGTAGCTAATG+CGG | 0.622898 | 7.4:+45547647 | None:intergenic |
| AAGAGGAAGAAGGGATACCC+TGG | 0.628274 | 7.4:+45547483 | None:intergenic |
| AGATACACAAGGTAGAATTG+AGG | 0.639080 | 7.4:-45547748 | MS.gene66111:CDS |
| GGATACCCTGGTGCACATTG+CGG | 0.656514 | 7.4:+45547495 | None:intergenic |
| TAATTCCGCAATGTGCACCA+GGG | 0.718364 | 7.4:-45547500 | MS.gene66111:CDS |
| AAGGATGTATCAGATACACA+AGG | 0.743090 | 7.4:-45547759 | MS.gene66111:CDS |
| TGAGGGTGAGGAAAATTACG+AGG | 0.746935 | 7.4:-45547793 | MS.gene66111:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAATGCTCTAAACATG+AGG | - | chr7.4:45547463-45547482 | MS.gene66111:CDS | 25.0% |
| !! | ATTAAATGCTCTAAACATGA+GGG | - | chr7.4:45547464-45547483 | MS.gene66111:CDS | 25.0% |
| AAAGTAATAGAAGAGGAAGA+AGG | + | chr7.4:45547804-45547823 | None:intergenic | 30.0% | |
| AAGTAATAGAAGAGGAAGAA+GGG | + | chr7.4:45547803-45547822 | None:intergenic | 30.0% | |
| GACAAAGAAAGTAATAGAAG+AGG | + | chr7.4:45547811-45547830 | None:intergenic | 30.0% | |
| ! | GGTTTCACTTTCAAAGTTTT+TGG | - | chr7.4:45547740-45547759 | MS.gene66111:CDS | 30.0% |
| ! | TGTCATTTGTTTCAACAGTT+TGG | - | chr7.4:45547827-45547846 | MS.gene66111:CDS | 30.0% |
| AAGGATGTATCAGATACACA+AGG | - | chr7.4:45547515-45547534 | MS.gene66111:CDS | 35.0% | |
| AGATACACAAGGTAGAATTG+AGG | - | chr7.4:45547526-45547545 | MS.gene66111:CDS | 35.0% | |
| ATTCTGCTTCTGTAATGCAA+AGG | + | chr7.4:45547446-45547465 | None:intergenic | 35.0% | |
| ! | TTACGAGGCGTTAAAATCTA+AGG | - | chr7.4:45547496-45547515 | MS.gene66111:CDS | 35.0% |
| AGAATTGAGGTGCAAACAGA+TGG | - | chr7.4:45547539-45547558 | MS.gene66111:CDS | 40.0% | |
| ATTCTCCAACAGCCTATTCT+AGG | - | chr7.4:45547650-45547669 | MS.gene66111:CDS | 40.0% | |
| GGATGCTATTGTAGCTAATG+CGG | + | chr7.4:45547630-45547649 | None:intergenic | 40.0% | |
| GTGCAAACAGATGGTAAAGA+GGG | - | chr7.4:45547548-45547567 | MS.gene66111:CDS | 40.0% | |
| TAGAATAGGCTGTTGGAGAA+TGG | + | chr7.4:45547651-45547670 | None:intergenic | 40.0% | |
| AAAGTGAAACCAGCAGCAAC+AGG | + | chr7.4:45547731-45547750 | None:intergenic | 45.0% | |
| ATAATTCCGCAATGTGCACC+AGG | - | chr7.4:45547773-45547792 | MS.gene66111:CDS | 45.0% | |
| ATCGCAATCATAGCCAGGAA+CGG | + | chr7.4:45547593-45547612 | None:intergenic | 45.0% | |
| GCTATGATTGCGATTGCGTT+AGG | - | chr7.4:45547599-45547618 | MS.gene66111:CDS | 45.0% | |
| GGTGCAAACAGATGGTAAAG+AGG | - | chr7.4:45547547-45547566 | MS.gene66111:CDS | 45.0% | |
| GTGATCCTAGAATAGGCTGT+TGG | + | chr7.4:45547658-45547677 | None:intergenic | 45.0% | |
| TAATTCCGCAATGTGCACCA+GGG | - | chr7.4:45547774-45547793 | MS.gene66111:CDS | 45.0% | |
| TGAGGGTGAGGAAAATTACG+AGG | - | chr7.4:45547481-45547500 | MS.gene66111:CDS | 45.0% | |
| ! | ATGCTCTAAACATGAGGGTG+AGG | - | chr7.4:45547469-45547488 | MS.gene66111:CDS | 45.0% |
| AACAGGAGCAACTGCTGAAG+AGG | + | chr7.4:45547714-45547733 | None:intergenic | 50.0% | |
| AAGAGGAAGAAGGGATACCC+TGG | + | chr7.4:45547794-45547813 | None:intergenic | 50.0% | |
| ACGCAATCGCAATCATAGCC+AGG | + | chr7.4:45547598-45547617 | None:intergenic | 50.0% | |
| CCAGACGGTGATCCTAGAAT+AGG | + | chr7.4:45547665-45547684 | None:intergenic | 50.0% | |
| GACAGAATCTGCAGACCAGA+CGG | + | chr7.4:45547680-45547699 | None:intergenic | 50.0% | |
| GGTCTGCAGATTCTGTCAGA+AGG | - | chr7.4:45547683-45547702 | MS.gene66111:CDS | 50.0% | |
| TCGCAATCATAGCCAGGAAC+GGG | + | chr7.4:45547592-45547611 | None:intergenic | 50.0% | |
| !! | CCTATTCTAGGATCACCGTC+TGG | - | chr7.4:45547662-45547681 | MS.gene66111:CDS | 50.0% |
| CAAGACAGAGATCCCGTTCC+TGG | - | chr7.4:45547577-45547596 | MS.gene66111:CDS | 55.0% | |
| GGATACCCTGGTGCACATTG+CGG | + | chr7.4:45547782-45547801 | None:intergenic | 55.0% | |
| GCAGTTGCTCCTGTTGCTGC+TGG | - | chr7.4:45547719-45547738 | MS.gene66111:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 45547434 | 45547862 | 45547434 | ID=MS.gene66111 |
| chr7.4 | mRNA | 45547434 | 45547862 | 45547434 | ID=MS.gene66111.t1;Parent=MS.gene66111 |
| chr7.4 | exon | 45547434 | 45547862 | 45547434 | ID=MS.gene66111.t1.exon1;Parent=MS.gene66111.t1 |
| chr7.4 | CDS | 45547434 | 45547862 | 45547434 | ID=cds.MS.gene66111.t1;Parent=MS.gene66111.t1 |
| Gene Sequence |
| Protein sequence |
