Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05592.t1 | XP_013460172.1 | 98.2 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 6.50E-22 | 113.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| No data available in table | |||||||||||
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05592.t1 | I3SG71 | 98.2 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 4.7e-22 | 113.2 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene05592 | 0.826667 | 2.39E-54 | -1.69E-46 |
| MS.gene049171 | MS.gene05592 | 0.838219 | 3.28E-57 | -1.69E-46 |
| MS.gene049173 | MS.gene05592 | 0.810233 | 1.28E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene05592 | 0.863582 | 2.25E-64 | -1.69E-46 |
| MS.gene049222 | MS.gene05592 | 0.852181 | 5.46E-61 | -1.69E-46 |
| MS.gene049232 | MS.gene05592 | 0.806948 | 6.42E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene05592 | 0.875459 | 3.03E-68 | -1.69E-46 |
| MS.gene049320 | MS.gene05592 | 0.874661 | 5.67E-68 | -1.69E-46 |
| MS.gene049356 | MS.gene05592 | 0.803937 | 2.75E-49 | -1.69E-46 |
| MS.gene049369 | MS.gene05592 | 0.852223 | 5.32E-61 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05592.t1 | MTR_3g462990 | 95.789 | 95 | 4 | 0 | 1 | 95 | 1 | 95 | 1.22e-54 | 164 |
| MS.gene05592.t1 | MTR_5g069990 | 59.016 | 61 | 13 | 1 | 32 | 80 | 32 | 92 | 5.23e-17 | 70.1 |
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCCTTTCTCTTCTTTGA+TGG | 0.353279 | 3.2:+53860184 | None:intergenic |
| AACTACTAGAACTAGAGTTT+TGG | 0.374057 | 3.2:-53860240 | MS.gene05592:CDS |
| AAGAAGAAGGGTGGTGGCCT+TGG | 0.398087 | 3.2:-53860026 | MS.gene05592:CDS |
| AAGAAGGGTGGTGGCCTTGG+TGG | 0.460503 | 3.2:-53860023 | MS.gene05592:CDS |
| AAGAAGGTGGAGGAGAAGAG+TGG | 0.587987 | 3.2:-53860098 | MS.gene05592:CDS |
| AAGAGAAAGGCTTTCTTGAT+TGG | 0.313083 | 3.2:-53860175 | MS.gene05592:CDS |
| AAGCCTTTCTCTTCTTTGAT+GGG | 0.471537 | 3.2:+53860185 | None:intergenic |
| AAGGTGGAGGAGAAGAGTGG+AGG | 0.646817 | 3.2:-53860095 | MS.gene05592:CDS |
| ACTCTAGTTCTAGTAGTTGT+TGG | 0.362442 | 3.2:+53860245 | None:intergenic |
| ACTTGGCAAACAAGCCACCA+AGG | 0.670213 | 3.2:+53860009 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGCCTTTCTCTTCTTTGA+TGG | + | chr3.2:53860098-53860117 | None:intergenic | 35.0% | |
| AAGAGAAAGGCTTTCTTGAT+TGG | - | chr3.2:53860104-53860123 | MS.gene05592:CDS | 35.0% | |
| AAGCCTTTCTCTTCTTTGAT+GGG | + | chr3.2:53860097-53860116 | None:intergenic | 35.0% | |
| TAGGATCAGTCTCAAAAAAC+TGG | + | chr3.2:53860156-53860175 | None:intergenic | 35.0% | |
| TTTCTTGATTGGATTCTTGG+AGG | - | chr3.2:53860115-53860134 | MS.gene05592:CDS | 35.0% | |
| GGCTTTCTTGATTGGATTCT+TGG | - | chr3.2:53860112-53860131 | MS.gene05592:CDS | 40.0% | |
| GACTGATCCTATCCTCAAGA+AGG | - | chr3.2:53860165-53860184 | MS.gene05592:CDS | 45.0% | |
| GATTCTTGGAGGAATGCAGA+AGG | - | chr3.2:53860126-53860145 | MS.gene05592:CDS | 45.0% | |
| GCACCCATCAAAGAAGAGAA+AGG | - | chr3.2:53860091-53860110 | MS.gene05592:CDS | 45.0% | |
| GTAGTACCCTTTGTTGCAGT+AGG | + | chr3.2:53860067-53860086 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 53860007 | 53860294 | 53860007 | ID=MS.gene05592 |
| chr3.2 | mRNA | 53860007 | 53860294 | 53860007 | ID=MS.gene05592.t1;Parent=MS.gene05592 |
| chr3.2 | exon | 53860007 | 53860294 | 53860007 | ID=MS.gene05592.t1.exon1;Parent=MS.gene05592.t1 |
| chr3.2 | CDS | 53860007 | 53860294 | 53860007 | ID=cds.MS.gene05592.t1;Parent=MS.gene05592.t1 |
| Gene Sequence |
>MS.gene05592 ATGACTTCATTGTTCATGTCATTTACTCCAACAACTACTAGAACTAGAGTTTTGGCACCTACTGCAACAAAGGGTACTACAAGTGCACCCATCAAAGAAGAGAAAGGCTTTCTTGATTGGATTCTTGGAGGAATGCAGAAGGAAGACCAGTTTTTTGAGACTGATCCTATCCTCAAGAAGGTGGAGGAGAAGAGTGGAGGAACCGCTAGCAACGGTCGCAAGAACACCGTGGTTGTGCCACCACAAAAGAAGAAGGGTGGTGGCCTTGGTGGCTTGTTTGCCAAGTGA |
| Protein sequence |
>MS.gene05592.t1 MTSLFMSFTPTTTRTRVLAPTATKGTTSAPIKEEKGFLDWILGGMQKEDQFFETDPILKKVEEKSGGTASNGRKNTVVVPPQKKKGGGLGGLFAK |
