Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66483.t1 | KEH40000.1 | 92.5 | 818 | 54 | 2 | 1 | 811 | 70 | 887 | 0.00E+00 | 1174.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66483.t1 | B6EUA9 | 51.5 | 818 | 345 | 9 | 3 | 811 | 30 | 804 | 1.6e-172 | 608.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66483.t1 | A0A072VPD9 | 92.5 | 818 | 54 | 2 | 1 | 811 | 70 | 887 | 0.0e+00 | 1174.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051669 | MS.gene66483 | 0.996951 | 1.31E-234 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene66483 | MS.gene74436 | PPI |
| MS.gene063673 | MS.gene66483 | PPI |
| MS.gene067412 | MS.gene66483 | PPI |
| MS.gene67503 | MS.gene66483 | PPI |
| MS.gene66483 | MS.gene074082 | PPI |
| MS.gene47154 | MS.gene66483 | PPI |
| MS.gene66483 | MS.gene64820 | PPI |
| MS.gene66483 | MS.gene67685 | PPI |
| MS.gene66483 | MS.gene83541 | PPI |
| MS.gene55513 | MS.gene66483 | PPI |
| MS.gene66483 | MS.gene86409 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66483.t1 | MTR_1g017520 | 97.538 | 975 | 17 | 2 | 1 | 968 | 70 | 1044 | 0.0 | 1956 |
| MS.gene66483.t1 | MTR_1g106025 | 52.658 | 978 | 399 | 25 | 17 | 968 | 64 | 1003 | 0.0 | 775 |
| MS.gene66483.t1 | MTR_4g074440 | 24.215 | 764 | 420 | 25 | 61 | 726 | 191 | 893 | 1.16e-33 | 140 |
| MS.gene66483.t1 | MTR_4g074440 | 25.047 | 535 | 280 | 19 | 61 | 527 | 191 | 672 | 3.65e-18 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66483.t1 | AT1G44910 | 56.090 | 977 | 368 | 19 | 3 | 966 | 30 | 958 | 0.0 | 882 |
| MS.gene66483.t1 | AT1G44910 | 55.816 | 937 | 357 | 17 | 3 | 926 | 30 | 922 | 0.0 | 838 |
| MS.gene66483.t1 | AT3G19670 | 42.281 | 991 | 496 | 24 | 1 | 965 | 50 | 990 | 0.0 | 634 |
| MS.gene66483.t1 | AT3G19670 | 42.281 | 991 | 496 | 24 | 1 | 965 | 50 | 990 | 0.0 | 634 |
| MS.gene66483.t1 | AT3G19670 | 42.281 | 991 | 496 | 24 | 1 | 965 | 50 | 990 | 0.0 | 634 |
| MS.gene66483.t1 | AT3G19670 | 42.281 | 991 | 496 | 24 | 1 | 965 | 31 | 971 | 0.0 | 632 |
| MS.gene66483.t1 | AT3G19840 | 25.212 | 353 | 224 | 10 | 410 | 732 | 454 | 796 | 2.57e-18 | 90.9 |
| MS.gene66483.t1 | AT3G19840 | 27.426 | 237 | 131 | 10 | 39 | 237 | 97 | 330 | 2.97e-11 | 68.2 |
Find 204 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCATGGCTTGCCTGTTTC+TGG | 0.038438 | 1.4:-5258218 | MS.gene66483:CDS |
| GATAATCAACGACCAGTTTC+TGG | 0.168664 | 1.4:-5254426 | MS.gene66483:CDS |
| AGCAGAATATAGGAAATTTC+TGG | 0.198110 | 1.4:-5250406 | MS.gene66483:CDS |
| GGCCTGTTTGTTTGAATATA+TGG | 0.213492 | 1.4:+5258296 | None:intergenic |
| AAATGCATTTAAAGCGCTTT+TGG | 0.225366 | 1.4:-5253459 | MS.gene66483:CDS |
| CCATATACCTTGAGTTCTTC+TGG | 0.251280 | 1.4:+5254711 | None:intergenic |
| ACATCCTCAAATAAATCTTT+TGG | 0.289484 | 1.4:+5247553 | None:intergenic |
| GGGACCACGGGGACGGTTCC+AGG | 0.299819 | 1.4:-5244628 | MS.gene66483:CDS |
| TTTGAGGCGGATGCTGGTAT+TGG | 0.308848 | 1.4:+5258039 | None:intergenic |
| TTGAGGCGGATGCTGGTATT+GGG | 0.320664 | 1.4:+5258040 | None:intergenic |
| TTTCAGTTCACACCATCTTA+TGG | 0.321948 | 1.4:-5258074 | MS.gene66483:intron |
| TTATCATCCATTCAGTATTT+GGG | 0.338884 | 1.4:-5253120 | MS.gene66483:intron |
| TACCAAAGTTTCACCATCAT+TGG | 0.343297 | 1.4:+5253620 | None:intergenic |
| CGTTCAGTTGGCCCGTGAGC+AGG | 0.344750 | 1.4:-5254619 | MS.gene66483:intron |
| CTCATTCCATCCAGTTCTCC+TGG | 0.384057 | 1.4:+5244610 | None:intergenic |
| TTCTGATTGGACGTGGGAAC+AGG | 0.389042 | 1.4:-5253420 | MS.gene66483:intron |
| CTGTGAATGTTCATTCTGAT+TGG | 0.390623 | 1.4:-5253433 | MS.gene66483:CDS |
| ACAGCAGTTGCAGGAAGCTT+AGG | 0.403204 | 1.4:-5254312 | MS.gene66483:CDS |
| TGAGGAGGTGCTCCAGAAAC+AGG | 0.404094 | 1.4:+5258206 | None:intergenic |
| GGGCTGATGCATCAACTGTT+TGG | 0.410617 | 1.4:-5254927 | MS.gene66483:CDS |
| GATATAGGAACGAGTCCGTC+GGG | 0.415488 | 1.4:-5247830 | MS.gene66483:intron |
| AGCTACTTGCAAAACAATAT+CGG | 0.417106 | 1.4:-5246827 | MS.gene66483:CDS |
| GGGCTTGAAGCAAGACCATT+AGG | 0.420839 | 1.4:+5254477 | None:intergenic |
| TGCCATATATTCAAACAAAC+AGG | 0.421533 | 1.4:-5258298 | MS.gene66483:CDS |
| ACCACTGATAATCCAGAAAC+TGG | 0.425854 | 1.4:+5254414 | None:intergenic |
| AGAGTACAAGGCAATCTAGT+TGG | 0.428021 | 1.4:-5255733 | MS.gene66483:CDS |
| AAGAAGGCATCAGAGCAGTA+TGG | 0.433242 | 1.4:-5244882 | MS.gene66483:CDS |
| GGAATGAGGAGCTTGAAGAT+GGG | 0.437118 | 1.4:-5244595 | MS.gene66483:CDS |
| ACTTCCTTATATAGTTCAAT+TGG | 0.438478 | 1.4:-5245585 | MS.gene66483:intron |
| AGCGACTACATCAGTATTTG+AGG | 0.439124 | 1.4:-5246875 | MS.gene66483:CDS |
| ACTGCACCTACAGCAGTTGC+AGG | 0.439764 | 1.4:-5254321 | MS.gene66483:CDS |
| TGGAGCACCTCTTCAAGATA+TGG | 0.442331 | 1.4:-5254101 | MS.gene66483:CDS |
| AGGTTACTCAGCAATCAGTA+TGG | 0.449369 | 1.4:-5254740 | MS.gene66483:CDS |
| AACCAATGATGGTGAAACTT+TGG | 0.452007 | 1.4:-5253622 | MS.gene66483:CDS |
| TGGAATGAGGAGCTTGAAGA+TGG | 0.452090 | 1.4:-5244596 | MS.gene66483:CDS |
| GATGCAACAGCTTGATATTG+AGG | 0.452557 | 1.4:+5247595 | None:intergenic |
| TCTTCTCCTCTCTATAACCA+TGG | 0.455879 | 1.4:+5244936 | None:intergenic |
| GCAGGAGGTGCATGCATTTG+AGG | 0.456721 | 1.4:+5258023 | None:intergenic |
| TACTCCAAAAGATTTATTTG+AGG | 0.462163 | 1.4:-5247557 | MS.gene66483:CDS |
| GTCGGGATGCCTGCTATTCA+AGG | 0.463184 | 1.4:-5258413 | MS.gene66483:CDS |
| CACGATTCTACTTCTGTCAA+TGG | 0.463402 | 1.4:-5254121 | MS.gene66483:CDS |
| GAGCTTGAAGATGGGGAGCT+TGG | 0.463687 | 1.4:-5244587 | MS.gene66483:CDS |
| GACGACCAAGGTTGTCCGGC+AGG | 0.464632 | 1.4:+5258005 | None:intergenic |
| TCCAACACTTTGAGACCTAA+TGG | 0.466267 | 1.4:-5254492 | MS.gene66483:CDS |
| CCCATGACTTCACAACAATT+CGG | 0.467987 | 1.4:-5258467 | MS.gene66483:CDS |
| GAGTACAAGGCAATCTAGTT+GGG | 0.468679 | 1.4:-5255732 | MS.gene66483:CDS |
| TGATTATCAGTGGCAACAAC+TGG | 0.471567 | 1.4:+5254441 | None:intergenic |
| AACGAGTCCGTCGGGGTGAA+AGG | 0.472697 | 1.4:-5247822 | MS.gene66483:CDS |
| TGAGGACGACCAAGGTTGTC+CGG | 0.473310 | 1.4:+5258001 | None:intergenic |
| CTTTGAAAATCTTGAAAAGC+AGG | 0.473646 | 1.4:-5247530 | MS.gene66483:intron |
| GGCACATAAAACTATTAGCC+AGG | 0.474323 | 1.4:-5254598 | MS.gene66483:CDS |
| TTGTTTCTGACACCATTCCC+TGG | 0.476710 | 1.4:+5254580 | None:intergenic |
| GGGGACGGTTCCAGGAGAAC+TGG | 0.476948 | 1.4:-5244620 | MS.gene66483:CDS |
| ACAACTGGTGTAACAGAAGA+TGG | 0.477825 | 1.4:+5254456 | None:intergenic |
| AGCAGAGGAGGCAGCAGCAT+TGG | 0.478603 | 1.4:+5254548 | None:intergenic |
| CCGGCTGGAGTTCAATCATC+TGG | 0.478668 | 1.4:-5257942 | MS.gene66483:CDS |
| GTTCACACCATCTTATGGTC+AGG | 0.487720 | 1.4:-5258069 | MS.gene66483:CDS |
| CCATTCAGTATTTGGGTCAA+AGG | 0.489226 | 1.4:-5253113 | MS.gene66483:intron |
| AATATCGGAGATCAATTTGA+AGG | 0.489301 | 1.4:-5246812 | MS.gene66483:intron |
| GGAATACATTACATACTTGA+AGG | 0.490150 | 1.4:-5245532 | MS.gene66483:CDS |
| CATAAAAGATATACTTAAGT+CGG | 0.491240 | 1.4:-5247004 | MS.gene66483:CDS |
| GCAACAACTCTGCATCTGAT+TGG | 0.493344 | 1.4:-5255957 | MS.gene66483:CDS |
| AAGCTTCCTGCAACTGCTGT+AGG | 0.493376 | 1.4:+5254315 | None:intergenic |
| CACCTCCTGCCGGACAACCT+TGG | 0.495114 | 1.4:-5258010 | MS.gene66483:CDS |
| GTGGCGAGATTATTGCTTGA+AGG | 0.495782 | 1.4:-5247728 | MS.gene66483:intron |
| CATCTTTGTGAATTCTTCGC+GGG | 0.495972 | 1.4:+5253047 | None:intergenic |
| GCAGGACTATATTCGTGACT+TGG | 0.496186 | 1.4:-5247961 | MS.gene66483:intron |
| TCACAACAATTCGGACATGC+AGG | 0.496394 | 1.4:-5258458 | MS.gene66483:CDS |
| TGACCGGGCTGAATTTGTCT+TGG | 0.497049 | 1.4:+5258350 | None:intergenic |
| TCATCCTGTTTCATCATCAC+AGG | 0.497226 | 1.4:-5258330 | MS.gene66483:CDS |
| ATAAAAGATATACTTAAGTC+GGG | 0.500700 | 1.4:-5247003 | MS.gene66483:CDS |
| ATTAGAGAGAGCTAAGGAGA+AGG | 0.502167 | 1.4:-5245926 | MS.gene66483:CDS |
| CCGTTCCTTCATCTAATGTC+GGG | 0.507422 | 1.4:-5258430 | MS.gene66483:CDS |
| GCCGTTCCTTCATCTAATGT+CGG | 0.508127 | 1.4:-5258431 | MS.gene66483:CDS |
| TTGGAGCTAGCTGCATTAGC+TGG | 0.508131 | 1.4:+5254510 | None:intergenic |
| AGAGAGAGCTAAGGAGAAGG+AGG | 0.508555 | 1.4:-5245923 | MS.gene66483:CDS |
| TATTATTACTTCTTCAACAT+GGG | 0.510330 | 1.4:-5245742 | MS.gene66483:CDS |
| AATTGCTGACCTTGAATAGC+AGG | 0.511307 | 1.4:+5258404 | None:intergenic |
| AGAGTTATTAGAGAGAGCTA+AGG | 0.512572 | 1.4:-5245932 | MS.gene66483:CDS |
| CATGCATTTGAGGCGGATGC+TGG | 0.512699 | 1.4:+5258033 | None:intergenic |
| GGCGGAATTTAGCAGAATAT+AGG | 0.514204 | 1.4:-5250416 | MS.gene66483:CDS |
| TTTGTTTGAATATATGGCAT+AGG | 0.515540 | 1.4:+5258302 | None:intergenic |
| AGTGCTTGGTTCGACTCCTA+TGG | 0.517790 | 1.4:+5254359 | None:intergenic |
| TCAGAGCAGTATGGAGGATG+TGG | 0.518136 | 1.4:-5244873 | MS.gene66483:CDS |
| CAAATGCATGCACCTCCTGC+CGG | 0.518916 | 1.4:-5258020 | MS.gene66483:CDS |
| CATGGTGGAACTTGAGAGAA+AGG | 0.520392 | 1.4:-5252697 | MS.gene66483:intron |
| AAAGACACAAGAGGGACCAC+GGG | 0.521871 | 1.4:-5244640 | MS.gene66483:CDS |
| CTAGAAGAACATATTGCTGA+TGG | 0.524243 | 1.4:-5247772 | MS.gene66483:CDS |
| TGCTTGAAGTAACGACAGAA+TGG | 0.525508 | 1.4:+5254382 | None:intergenic |
| CTCACAGTTACAACAGTGCT+TGG | 0.526579 | 1.4:+5254345 | None:intergenic |
| ACATCTTTGTGAATTCTTCG+CGG | 0.526596 | 1.4:+5253046 | None:intergenic |
| GTAACGTCTGTACAGCCGGC+TGG | 0.529488 | 1.4:-5257957 | MS.gene66483:CDS |
| CAAGAGCATACTGCTGGTGA+TGG | 0.531052 | 1.4:-5255934 | MS.gene66483:CDS |
| TTTGGTATATGCAAATAAGC+TGG | 0.531455 | 1.4:-5253604 | MS.gene66483:intron |
| AAGAGAGAAAACATCGAAGA+AGG | 0.532161 | 1.4:-5244898 | MS.gene66483:CDS |
| ACCATTAGGTCTCAAAGTGT+TGG | 0.536875 | 1.4:+5254491 | None:intergenic |
| TTCTCAACAAATGCAACAGT+TGG | 0.538488 | 1.4:-5258375 | MS.gene66483:CDS |
| CTGTTGAAAGAGTAAGAGAT+CGG | 0.540036 | 1.4:-5252740 | MS.gene66483:CDS |
| AGATTTATTTGAAAGCTACA+TGG | 0.541453 | 1.4:-5252715 | MS.gene66483:CDS |
| CGTACAGTGTATAGAGATTG+AGG | 0.543077 | 1.4:+5258188 | None:intergenic |
| TGATGATGAAACAGGATGAC+CGG | 0.543456 | 1.4:+5258334 | None:intergenic |
| AGCTGTAACGTCTGTACAGC+CGG | 0.544674 | 1.4:-5257961 | MS.gene66483:CDS |
| AGAAGCTAGAGGCTGAAGAA+AGG | 0.544989 | 1.4:-5253089 | MS.gene66483:CDS |
| ACGTAAGCGTGAAGAAGAAA+AGG | 0.545043 | 1.4:-5245490 | MS.gene66483:intron |
| TGATATAGGAACGAGTCCGT+CGG | 0.545937 | 1.4:-5247831 | MS.gene66483:intron |
| TGACTTGGAGAAGGAGGAAG+AGG | 0.546026 | 1.4:-5247946 | MS.gene66483:CDS |
| ATACTGCTGGTGATGGGAGA+AGG | 0.548219 | 1.4:-5255927 | MS.gene66483:intron |
| GCTGTTGCATCAAATACATC+CGG | 0.551042 | 1.4:-5247583 | MS.gene66483:CDS |
| GAAGTGACCGCAAGAAGTCT+AGG | 0.551692 | 1.4:-5244802 | MS.gene66483:CDS |
| GGAGTTGATGTCACCTCTTG+AGG | 0.553125 | 1.4:-5255699 | MS.gene66483:intron |
| ATACCTTCCTAGACTTCTTG+CGG | 0.553463 | 1.4:+5244795 | None:intergenic |
| GAAAGAGTTCACTTCTTCAG+AGG | 0.554876 | 1.4:-5254905 | MS.gene66483:CDS |
| TTCAACATGGGAGGAATGCA+AGG | 0.556166 | 1.4:-5245730 | MS.gene66483:CDS |
| TCTAGAAAATCGCGAAGACA+TGG | 0.556892 | 1.4:-5244824 | MS.gene66483:CDS |
| ATACCCTGTGATGATGAAAC+AGG | 0.557450 | 1.4:+5258326 | None:intergenic |
| CAGGAGAACTGGATGGAATG+AGG | 0.557842 | 1.4:-5244609 | MS.gene66483:CDS |
| TCCGAATTGTTGTGAAGTCA+TGG | 0.558122 | 1.4:+5258466 | None:intergenic |
| GAGCTAGCTGCATTAGCTGG+TGG | 0.559039 | 1.4:+5254513 | None:intergenic |
| GCTTGAAGTAACGACAGAAT+GGG | 0.559357 | 1.4:+5254383 | None:intergenic |
| TAAAGTCATCAGCAAGGCGT+TGG | 0.560032 | 1.4:+5245881 | None:intergenic |
| AGTTGATGTTGCAGCAGAGG+AGG | 0.561689 | 1.4:+5254536 | None:intergenic |
| TCTCCTGAGTCAGACAATGA+AGG | 0.561975 | 1.4:-5244671 | MS.gene66483:CDS |
| AAAAGACACAAGAGGGACCA+CGG | 0.563924 | 1.4:-5244641 | MS.gene66483:CDS |
| TCAGAGGAGAAAACCAATGA+TGG | 0.566057 | 1.4:-5253633 | MS.gene66483:CDS |
| TAAAACTATTAGCCAGGGAA+TGG | 0.567415 | 1.4:-5254592 | MS.gene66483:CDS |
| CTACCTTCATTGTCTGACTC+AGG | 0.567758 | 1.4:+5244668 | None:intergenic |
| TATATTCTGCTAAATTCCGC+CGG | 0.569486 | 1.4:+5250418 | None:intergenic |
| AGCTAACATCATCCACAAGA+TGG | 0.570809 | 1.4:-5252904 | MS.gene66483:CDS |
| GTCGTTACTTCAAGCACCAT+AGG | 0.571185 | 1.4:-5254375 | MS.gene66483:CDS |
| CTATATTCGTGACTTGGAGA+AGG | 0.573107 | 1.4:-5247955 | MS.gene66483:CDS |
| CACATAAACAATCATATGCA+TGG | 0.577352 | 1.4:-5258233 | MS.gene66483:CDS |
| TATTACTTCTTCAACATGGG+AGG | 0.581858 | 1.4:-5245739 | MS.gene66483:CDS |
| AAAGAGTTCACTTCTTCAGA+GGG | 0.582420 | 1.4:-5254904 | MS.gene66483:CDS |
| GGAAAAGGAAAATGCTGCTG+AGG | 0.585150 | 1.4:-5250445 | MS.gene66483:CDS |
| ATGTACCTTCACAAAATCAC+AGG | 0.586297 | 1.4:+5250380 | None:intergenic |
| CCAGATGATTGAACTCCAGC+CGG | 0.587420 | 1.4:+5257942 | None:intergenic |
| TATAATGCCCTGAAAACACT+TGG | 0.587574 | 1.4:-5253290 | MS.gene66483:CDS |
| CTGCAGGTATTATTACAACA+AGG | 0.589073 | 1.4:-5254760 | MS.gene66483:intron |
| TGTTGAAAGAGTAAGAGATC+GGG | 0.589167 | 1.4:-5252739 | MS.gene66483:CDS |
| GAATGAGGAGCTTGAAGATG+GGG | 0.589298 | 1.4:-5244594 | MS.gene66483:CDS |
| ACATTAGTTTGTCCAACCAC+AGG | 0.594069 | 1.4:+5253663 | None:intergenic |
| GATTGGCAAGAGCATACTGC+TGG | 0.595223 | 1.4:-5255940 | MS.gene66483:CDS |
| GACAATACCAGAAGAACTCA+AGG | 0.597189 | 1.4:-5254718 | MS.gene66483:intron |
| ATGTTCATTCTGATTGGACG+TGG | 0.597884 | 1.4:-5253427 | MS.gene66483:CDS |
| CACAAGAGGGACCACGGGGA+CGG | 0.599081 | 1.4:-5244635 | MS.gene66483:CDS |
| AATACCTCCATATCTTGAAG+AGG | 0.599572 | 1.4:+5254094 | None:intergenic |
| AGATATACTTAAGTCGGGCA+AGG | 0.600167 | 1.4:-5246998 | MS.gene66483:intron |
| AAAATGCTGCTGAGGAACAC+CGG | 0.602673 | 1.4:-5250437 | MS.gene66483:CDS |
| CAACTGGTGTAACAGAAGAT+GGG | 0.603013 | 1.4:+5254457 | None:intergenic |
| GGCATCCCGACATTAGATGA+AGG | 0.603036 | 1.4:+5258425 | None:intergenic |
| TAACTTATTATATACACTCA+AGG | 0.603685 | 1.4:-5245857 | MS.gene66483:intron |
| ATTACTACAACAAGAGTACA+AGG | 0.603856 | 1.4:-5255745 | MS.gene66483:CDS |
| AAATACTGATGTAGTCGCTA+CGG | 0.604006 | 1.4:+5246879 | None:intergenic |
| CCCGACATTAGATGAAGGAA+CGG | 0.604025 | 1.4:+5258430 | None:intergenic |
| AACTAATGTGACTCCTTCAG+AGG | 0.605322 | 1.4:-5253649 | MS.gene66483:CDS |
| TGGAGTTGATGTTGCAGCAG+AGG | 0.607065 | 1.4:+5254533 | None:intergenic |
| ACAGCTTGCTGTGCATGTTG+TGG | 0.607782 | 1.4:+5258260 | None:intergenic |
| AAAATTATACCTCCATCTTG+TGG | 0.609019 | 1.4:+5252892 | None:intergenic |
| ACGGTTCCAGGAGAACTGGA+TGG | 0.609038 | 1.4:-5244616 | MS.gene66483:CDS |
| TGAGGCGGATGCTGGTATTG+GGG | 0.609946 | 1.4:+5258041 | None:intergenic |
| AACTGGTCGTTGATTATCAG+TGG | 0.611617 | 1.4:+5254431 | None:intergenic |
| ATATTATTACTTCTTCAACA+TGG | 0.611639 | 1.4:-5245743 | MS.gene66483:CDS |
| TTTATTTGAAAGCTACATGG+TGG | 0.617248 | 1.4:-5252712 | MS.gene66483:CDS |
| ACCAGTTTCTGGATTATCAG+TGG | 0.617569 | 1.4:-5254415 | MS.gene66483:CDS |
| TGCATATGATTGTTTATGTG+AGG | 0.618480 | 1.4:+5258236 | None:intergenic |
| CACAACAATTCGGACATGCA+GGG | 0.622894 | 1.4:-5258457 | MS.gene66483:CDS |
| AGATGCAGAGTTGTTGCTAG+TGG | 0.623592 | 1.4:+5255963 | None:intergenic |
| AAGAGCATACTGCTGGTGAT+GGG | 0.629015 | 1.4:-5255933 | MS.gene66483:CDS |
| GACCAAGGTTGTCCGGCAGG+AGG | 0.629097 | 1.4:+5258008 | None:intergenic |
| CTGAGTCAGACAATGAAGGT+AGG | 0.631523 | 1.4:-5244667 | MS.gene66483:CDS |
| GGCAATGCCTGACCATAAGA+TGG | 0.632811 | 1.4:+5258062 | None:intergenic |
| GGAGCTTGGCGATGATGCAG+AGG | 0.632857 | 1.4:-5244573 | MS.gene66483:CDS |
| TTCTCCTGGAACCGTCCCCG+TGG | 0.634316 | 1.4:+5244624 | None:intergenic |
| TATTCGTGACTTGGAGAAGG+AGG | 0.635163 | 1.4:-5247952 | MS.gene66483:CDS |
| GCTCCAAGACAAATTCAGCC+CGG | 0.637474 | 1.4:-5258353 | MS.gene66483:CDS |
| AGAGGTGACATCAACTCCAA+AGG | 0.638169 | 1.4:+5255704 | None:intergenic |
| CATCCTGTTTCATCATCACA+GGG | 0.640900 | 1.4:-5258329 | MS.gene66483:CDS |
| CAAGACATGACTGACAGCCA+TGG | 0.642401 | 1.4:-5244953 | MS.gene66483:CDS |
| TAGGCATAAAAGACACAAGA+GGG | 0.646717 | 1.4:-5244648 | MS.gene66483:CDS |
| TGACCGCAAGAAGTCTAGGA+AGG | 0.647233 | 1.4:-5244798 | MS.gene66483:intron |
| GCAACTTGCGAAATGCATCA+CGG | 0.647762 | 1.4:+5247794 | None:intergenic |
| AAGGCATCAGAGCAGTATGG+AGG | 0.652072 | 1.4:-5244879 | MS.gene66483:CDS |
| GCACATAAAACTATTAGCCA+GGG | 0.652408 | 1.4:-5254597 | MS.gene66483:CDS |
| AGTTAGTAAAGTCATCAGCA+AGG | 0.652933 | 1.4:+5245875 | None:intergenic |
| GGAGGTGCATGCATTTGAGG+CGG | 0.653398 | 1.4:+5258026 | None:intergenic |
| TGACAGCCATGGTTATAGAG+AGG | 0.655215 | 1.4:-5244942 | MS.gene66483:CDS |
| AGCACCTCTTCAAGATATGG+AGG | 0.656627 | 1.4:-5254098 | MS.gene66483:intron |
| CCGAATTGTTGTGAAGTCAT+GGG | 0.660354 | 1.4:+5258467 | None:intergenic |
| GATGATGAAACAGGATGACC+GGG | 0.660914 | 1.4:+5258335 | None:intergenic |
| TTACCTGGAAAACAAGCAAG+CGG | 0.662974 | 1.4:+5250190 | None:intergenic |
| GTTACAGCTGCAGCACTCTG+AGG | 0.670283 | 1.4:+5257975 | None:intergenic |
| TAACATCATCCACAAGATGG+AGG | 0.670995 | 1.4:-5252901 | MS.gene66483:intron |
| TGCAGCACTCTGAGGTACTG+AGG | 0.673442 | 1.4:+5257983 | None:intergenic |
| GGGTCAAAGGAAGAAGCTAG+AGG | 0.686231 | 1.4:-5253100 | MS.gene66483:CDS |
| TGAGGTACTGAGGACGACCA+AGG | 0.686869 | 1.4:+5257993 | None:intergenic |
| ATATAGGAACGAGTCCGTCG+GGG | 0.690250 | 1.4:-5247829 | MS.gene66483:intron |
| GTAGGCATAAAAGACACAAG+AGG | 0.702923 | 1.4:-5244649 | MS.gene66483:CDS |
| TGTTCATTCTGATTGGACGT+GGG | 0.712631 | 1.4:-5253426 | MS.gene66483:CDS |
| TGTTGTGGAACAGATGTCAG+TGG | 0.716753 | 1.4:+5258275 | None:intergenic |
| AAGACACAAGAGGGACCACG+GGG | 0.722637 | 1.4:-5244639 | MS.gene66483:CDS |
| ATGCTGCTGAGGAACACCGG+CGG | 0.733976 | 1.4:-5250434 | MS.gene66483:CDS |
| ACAGTGTATAGAGATTGAGG+AGG | 0.757065 | 1.4:+5258191 | None:intergenic |
| TTCTTACTGCTAAAACACAG+TGG | 0.768613 | 1.4:-5247747 | MS.gene66483:CDS |
| GCGAAAACAAGCTTTCAACG+AGG | 0.790814 | 1.4:-5253264 | MS.gene66483:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 5244568 | 5258486 | 5244568 | ID=MS.gene66483 |
| chr1.4 | mRNA | 5244568 | 5258486 | 5244568 | ID=MS.gene66483.t1;Parent=MS.gene66483 |
| chr1.4 | exon | 5258193 | 5258486 | 5258193 | ID=MS.gene66483.t1.exon1;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5258193 | 5258486 | 5258193 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5257923 | 5258090 | 5257923 | ID=MS.gene66483.t1.exon2;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5257923 | 5258090 | 5257923 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5255928 | 5255995 | 5255928 | ID=MS.gene66483.t1.exon3;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5255928 | 5255995 | 5255928 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5255700 | 5255769 | 5255700 | ID=MS.gene66483.t1.exon4;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5255700 | 5255769 | 5255700 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5254898 | 5254950 | 5254898 | ID=MS.gene66483.t1.exon5;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5254898 | 5254950 | 5254898 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5254719 | 5254776 | 5254719 | ID=MS.gene66483.t1.exon6;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5254719 | 5254776 | 5254719 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5254273 | 5254634 | 5254273 | ID=MS.gene66483.t1.exon7;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5254273 | 5254634 | 5254273 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5254099 | 5254162 | 5254099 | ID=MS.gene66483.t1.exon8;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5254099 | 5254162 | 5254099 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5253605 | 5253703 | 5253605 | ID=MS.gene66483.t1.exon9;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5253605 | 5253703 | 5253605 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5253421 | 5253489 | 5253421 | ID=MS.gene66483.t1.exon10;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5253421 | 5253489 | 5253421 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5253265 | 5253342 | 5253265 | ID=MS.gene66483.t1.exon11;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5253265 | 5253342 | 5253265 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5253041 | 5253127 | 5253041 | ID=MS.gene66483.t1.exon12;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5253041 | 5253127 | 5253041 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5252902 | 5252936 | 5252902 | ID=MS.gene66483.t1.exon13;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5252902 | 5252936 | 5252902 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5252698 | 5252803 | 5252698 | ID=MS.gene66483.t1.exon14;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5252698 | 5252803 | 5252698 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5250386 | 5250466 | 5250386 | ID=MS.gene66483.t1.exon15;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5250386 | 5250466 | 5250386 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5250194 | 5250286 | 5250194 | ID=MS.gene66483.t1.exon16;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5250194 | 5250286 | 5250194 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5247926 | 5247979 | 5247926 | ID=MS.gene66483.t1.exon17;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5247926 | 5247979 | 5247926 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5247729 | 5247845 | 5247729 | ID=MS.gene66483.t1.exon18;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5247729 | 5247845 | 5247729 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5247531 | 5247629 | 5247531 | ID=MS.gene66483.t1.exon19;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5247531 | 5247629 | 5247531 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5246999 | 5247046 | 5246999 | ID=MS.gene66483.t1.exon20;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5246999 | 5247046 | 5246999 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5246813 | 5246905 | 5246813 | ID=MS.gene66483.t1.exon21;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5246813 | 5246905 | 5246813 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5245858 | 5245965 | 5245858 | ID=MS.gene66483.t1.exon22;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5245858 | 5245965 | 5245858 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5245702 | 5245766 | 5245702 | ID=MS.gene66483.t1.exon23;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5245702 | 5245766 | 5245702 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5245491 | 5245593 | 5245491 | ID=MS.gene66483.t1.exon24;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5245491 | 5245593 | 5245491 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5244799 | 5245104 | 5244799 | ID=MS.gene66483.t1.exon25;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5244799 | 5245104 | 5244799 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| chr1.4 | exon | 5244568 | 5244702 | 5244568 | ID=MS.gene66483.t1.exon26;Parent=MS.gene66483.t1 |
| chr1.4 | CDS | 5244568 | 5244702 | 5244568 | ID=cds.MS.gene66483.t1;Parent=MS.gene66483.t1 |
| Gene Sequence |
| Protein sequence |
