Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66726.t1 | XP_013455799.1 | 99.1 | 564 | 5 | 0 | 1 | 564 | 1 | 564 | 0.00E+00 | 1106.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66726.t1 | Q8VCM8 | 33.1 | 537 | 307 | 14 | 20 | 532 | 28 | 536 | 1.8e-67 | 258.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66726.t1 | A0A072UVE5 | 99.1 | 564 | 5 | 0 | 1 | 564 | 1 | 564 | 0.0e+00 | 1106.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene28821 | MS.gene66726 | PPI |
| MS.gene07068 | MS.gene66726 | PPI |
| MS.gene072688 | MS.gene66726 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66726.t1 | MTR_4g052530 | 99.113 | 564 | 5 | 0 | 1 | 564 | 1 | 564 | 0.0 | 1142 |
| MS.gene66726.t1 | MTR_4g052530 | 95.719 | 584 | 5 | 1 | 1 | 564 | 1 | 584 | 0.0 | 1132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66726.t1 | AT3G44330 | 73.898 | 567 | 139 | 4 | 1 | 562 | 1 | 563 | 0.0 | 852 |
Find 121 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACATTGACACCGTGTTAT+TGG | 0.199855 | 4.3:-47413766 | MS.gene66726:CDS |
| AATTGTGCTCTTCAGTTTCT+TGG | 0.209041 | 4.3:-47407067 | MS.gene66726:CDS |
| TCAAGTGTTGCAGAAGATTT+AGG | 0.221551 | 4.3:-47411379 | MS.gene66726:CDS |
| CTTTGCGGACAACAGTAATT+TGG | 0.260388 | 4.3:-47408093 | MS.gene66726:CDS |
| CACATTCTTCAGCGGTTCTT+TGG | 0.275300 | 4.3:+47414927 | None:intergenic |
| TTCTAGAATTTGTTTAATAA+AGG | 0.282297 | 4.3:+47411506 | None:intergenic |
| GGTGGAGATCCAACTTTCTT+TGG | 0.305838 | 4.3:+47413440 | None:intergenic |
| ATGATCTAATCAGTTTATTT+CGG | 0.307918 | 4.3:-47406933 | MS.gene66726:CDS |
| CCGGTGCTCTGTAGCAATTC+AGG | 0.309146 | 4.3:+47410217 | None:intergenic |
| CTCAACATGTCCTTTGTTCA+AGG | 0.340657 | 4.3:-47416368 | MS.gene66726:intron |
| TCCACCCCTTACAAATATTC+AGG | 0.348535 | 4.3:-47413423 | MS.gene66726:intron |
| TGGCATCATATGACACCTTT+GGG | 0.352355 | 4.3:-47412430 | MS.gene66726:CDS |
| GGACAATACAATCTGCTGTT+TGG | 0.356916 | 4.3:-47412214 | MS.gene66726:CDS |
| GATGAAGACGAGTGCGATTA+CGG | 0.357448 | 4.3:+47416577 | None:intergenic |
| TGGTGCTACTCGAGGTGTTC+GGG | 0.368397 | 4.3:+47408028 | None:intergenic |
| GCTCATGGGAAAATGATCTA+TGG | 0.378313 | 4.3:-47411557 | MS.gene66726:CDS |
| AGCACCGGTGGTTTGGTTGA+TGG | 0.380580 | 4.3:-47410202 | MS.gene66726:CDS |
| CGGGTTCCATTGTAGTTGTA+AGG | 0.382485 | 4.3:+47412175 | None:intergenic |
| GATATCTCCGGTGTTCCGTT+TGG | 0.384689 | 4.3:-47416488 | MS.gene66726:CDS |
| GACAATACAATCTGCTGTTT+GGG | 0.385785 | 4.3:-47412213 | MS.gene66726:CDS |
| TTGTATTGTCCTCTTGTCTT+CGG | 0.385886 | 4.3:+47412226 | None:intergenic |
| ACATCTGCTGTAGGTAGCTT+GGG | 0.388643 | 4.3:-47410302 | MS.gene66726:intron |
| CTTGCTGTTGCTTCTAGTGT+TGG | 0.389075 | 4.3:-47407100 | MS.gene66726:CDS |
| GACATCTGCTGTAGGTAGCT+TGG | 0.390955 | 4.3:-47410303 | MS.gene66726:intron |
| CAAACACCTGAATATTTGTA+AGG | 0.392964 | 4.3:+47413417 | None:intergenic |
| GCTATTTGCTTAGATAGTAT+TGG | 0.394414 | 4.3:-47411579 | MS.gene66726:CDS |
| GTGGCATCATATGACACCTT+TGG | 0.400492 | 4.3:-47412431 | MS.gene66726:CDS |
| TTGCTGTTGCTTCTAGTGTT+GGG | 0.402441 | 4.3:-47407099 | MS.gene66726:CDS |
| ATTACCCACCATTGCGATAG+TGG | 0.410780 | 4.3:-47412450 | MS.gene66726:CDS |
| GCTACAGAGCACCGGTGGTT+TGG | 0.416449 | 4.3:-47410209 | MS.gene66726:CDS |
| TGGTTGCAGGGATGGTTGCC+AGG | 0.416491 | 4.3:-47412500 | MS.gene66726:intron |
| ATCCTTCTTATATTCGATCA+TGG | 0.419096 | 4.3:-47408064 | MS.gene66726:CDS |
| CTACAACGAGAAGTGCTAGA+TGG | 0.420471 | 4.3:-47407546 | MS.gene66726:CDS |
| TCCTCAAAAGCAAAATAAAC+AGG | 0.426831 | 4.3:+47413792 | None:intergenic |
| TATTGGTGGTAGCAAGCCTC+TGG | 0.428171 | 4.3:-47415028 | MS.gene66726:CDS |
| AACAGGATGTATCACCTTAC+GGG | 0.437949 | 4.3:+47412156 | None:intergenic |
| TCCAACTTTCTTTGGTTCAG+TGG | 0.446635 | 4.3:+47413448 | None:intergenic |
| CCGGTGGTTTGGTTGATGGC+AGG | 0.449964 | 4.3:-47410198 | MS.gene66726:intron |
| ATTGGTGGTAGCAAGCCTCT+GGG | 0.454869 | 4.3:-47415027 | MS.gene66726:CDS |
| TCTTATGCTTCAGATTAACT+TGG | 0.460383 | 4.3:+47411353 | None:intergenic |
| AGCACAGCTGAACATATATC+AGG | 0.467914 | 4.3:-47407496 | MS.gene66726:intron |
| ATGGTGCTACTCGAGGTGTT+CGG | 0.469594 | 4.3:+47408027 | None:intergenic |
| CTTAGATAGTATTGGCTCAT+GGG | 0.470218 | 4.3:-47411571 | MS.gene66726:CDS |
| CTGCTGTTTGGGCTAACGTC+TGG | 0.478814 | 4.3:-47412202 | MS.gene66726:CDS |
| TGGTGGTAGCAAGCCTCTGG+GGG | 0.481056 | 4.3:-47415025 | MS.gene66726:CDS |
| GCTTAGATAGTATTGGCTCA+TGG | 0.482039 | 4.3:-47411572 | MS.gene66726:CDS |
| TGACACCTTTGGGGCTGCTC+CGG | 0.485813 | 4.3:-47412420 | MS.gene66726:intron |
| CGGAGATATCGTATTGGATA+AGG | 0.488170 | 4.3:+47416501 | None:intergenic |
| GCCACTGAACCAAAGAAAGT+TGG | 0.489173 | 4.3:-47413449 | MS.gene66726:CDS |
| AAATTTACTCACAGGTAAGT+TGG | 0.502805 | 4.3:+47414873 | None:intergenic |
| CTGTTTGGGCTAACGTCTGG+TGG | 0.508872 | 4.3:-47412199 | MS.gene66726:CDS |
| TCACCATCTGTCTTCAGCCC+TGG | 0.516595 | 4.3:+47412482 | None:intergenic |
| AACCATGATCGAATATAAGA+AGG | 0.520778 | 4.3:+47408062 | None:intergenic |
| CCGCCAGTAGTTGCAGTAGC+AGG | 0.521090 | 4.3:+47413714 | None:intergenic |
| AAGCATATTTATGGGCATCA+AGG | 0.529020 | 4.3:-47408131 | MS.gene66726:CDS |
| CAGCCTGCTACTGCAACTAC+TGG | 0.535144 | 4.3:-47413717 | MS.gene66726:CDS |
| TACAACGAGAAGTGCTAGAT+GGG | 0.535738 | 4.3:-47407545 | MS.gene66726:CDS |
| ACTTGTACGACAGAGTATAT+TGG | 0.551265 | 4.3:-47415045 | MS.gene66726:intron |
| TCGTTGTAGATTAACTTCAT+CGG | 0.552366 | 4.3:+47407560 | None:intergenic |
| ACCTGAATATTTGTAAGGGG+TGG | 0.552902 | 4.3:+47413422 | None:intergenic |
| CACCCTCTCTGAACTCTCTA+CGG | 0.557086 | 4.3:-47410242 | MS.gene66726:CDS |
| CGGAGTCGAAGAGGATTTCG+CGG | 0.558974 | 4.3:+47416597 | None:intergenic |
| ATCAAGAAGAATGATGCCTC+TGG | 0.560007 | 4.3:-47413741 | MS.gene66726:CDS |
| TGATGCCACTATCGCAATGG+TGG | 0.566504 | 4.3:+47412445 | None:intergenic |
| ATCTAATCAGTTTATTTCGG+CGG | 0.568543 | 4.3:-47406930 | MS.gene66726:CDS |
| CAGCAAGTCAAACGTGACAC+TGG | 0.568984 | 4.3:+47407116 | None:intergenic |
| GCGAGTAGTAATCATGATGA+TGG | 0.569086 | 4.3:-47414952 | MS.gene66726:CDS |
| AAACACCTGAATATTTGTAA+GGG | 0.570541 | 4.3:+47413418 | None:intergenic |
| AAAGAACGTACAGATCTTTG+CGG | 0.571521 | 4.3:-47408108 | MS.gene66726:CDS |
| CTTATCCAATACGATATCTC+CGG | 0.573897 | 4.3:-47416500 | MS.gene66726:CDS |
| ACGAGATCCAAACGGAACAC+CGG | 0.575932 | 4.3:+47416481 | None:intergenic |
| TATTCGAATCCGAAGACAAG+AGG | 0.577516 | 4.3:-47412235 | MS.gene66726:CDS |
| CTGCTACTGCAACTACTGGC+GGG | 0.584909 | 4.3:-47413713 | MS.gene66726:intron |
| AAGAGGATTTCGCGGTTGCG+AGG | 0.587745 | 4.3:+47416605 | None:intergenic |
| GGAAGCGATAGCAATGGAAG+TGG | 0.595409 | 4.3:-47412301 | MS.gene66726:CDS |
| CAACTACAATGGAACCCGTA+AGG | 0.596945 | 4.3:-47412170 | MS.gene66726:intron |
| CGTAGAGAGTTCAGAGAGGG+TGG | 0.599324 | 4.3:+47410243 | None:intergenic |
| GAACACCGGAGATATCGTAT+TGG | 0.602447 | 4.3:+47416495 | None:intergenic |
| ATATATGTTCAGCTGTGCTG+TGG | 0.602643 | 4.3:+47407500 | None:intergenic |
| TGTTTGGGCTAACGTCTGGT+GGG | 0.605689 | 4.3:-47412198 | MS.gene66726:CDS |
| TTGGTGGTAGCAAGCCTCTG+GGG | 0.607148 | 4.3:-47415026 | MS.gene66726:CDS |
| AAATTGCGAACCTTGAACAA+AGG | 0.607154 | 4.3:+47416358 | None:intergenic |
| GTGCGATTACGGAGTCGAAG+AGG | 0.609499 | 4.3:+47416588 | None:intergenic |
| TGCAGTAGCAGGCTGACCAG+AGG | 0.611293 | 4.3:+47413725 | None:intergenic |
| GGTGGTAGCAAGCCTCTGGG+GGG | 0.611979 | 4.3:-47415024 | MS.gene66726:CDS |
| GGTTGCAGGGATGGTTGCCA+GGG | 0.612576 | 4.3:-47412499 | MS.gene66726:intron |
| CTAGCGAGACGAGATCCAAA+CGG | 0.613160 | 4.3:+47416473 | None:intergenic |
| GCCTGCGTCGACCTCTGTGA+CGG | 0.615505 | 4.3:-47416548 | MS.gene66726:CDS |
| TAATTGTAGGCGTTATCAGT+GGG | 0.618232 | 4.3:-47412322 | MS.gene66726:intron |
| GGTGGGCCTTACAACTACAA+TGG | 0.619306 | 4.3:-47412181 | MS.gene66726:CDS |
| TGCCGTAGAGAGTTCAGAGA+GGG | 0.620583 | 4.3:+47410240 | None:intergenic |
| AGAACCGCTGAAGAATGTGT+TGG | 0.621201 | 4.3:-47414923 | MS.gene66726:CDS |
| CCTGCCATCAACCAAACCAC+CGG | 0.622459 | 4.3:+47410198 | None:intergenic |
| CTTCTTGATATCCAATAACA+CGG | 0.623420 | 4.3:+47413755 | None:intergenic |
| AACACCTGAATATTTGTAAG+GGG | 0.627059 | 4.3:+47413419 | None:intergenic |
| GCCGTCACAGAGGTCGACGC+AGG | 0.628037 | 4.3:+47416547 | None:intergenic |
| GTAATTGTAGGCGTTATCAG+TGG | 0.631414 | 4.3:-47412323 | MS.gene66726:intron |
| CCTGAATTGCTACAGAGCAC+CGG | 0.633915 | 4.3:-47410217 | MS.gene66726:CDS |
| ACATACCGGAGCAGCCCCAA+AGG | 0.637663 | 4.3:+47412415 | None:intergenic |
| CGACGGTGGCGCCGTCACAG+AGG | 0.638908 | 4.3:+47416537 | None:intergenic |
| GGCATCATATGACACCTTTG+GGG | 0.639014 | 4.3:-47412429 | MS.gene66726:CDS |
| ACTAATTGCTGAGTCTCTTG+CGG | 0.641944 | 4.3:-47410062 | MS.gene66726:intron |
| CCTGCTACTGCAACTACTGG+CGG | 0.643000 | 4.3:-47413714 | MS.gene66726:CDS |
| TGTACGACAGAGTATATTGG+TGG | 0.643856 | 4.3:-47415042 | MS.gene66726:intron |
| GATGCCACTATCGCAATGGT+GGG | 0.644935 | 4.3:+47412446 | None:intergenic |
| TAGCAATGGAAGTGGCATTG+TGG | 0.650973 | 4.3:-47412293 | MS.gene66726:CDS |
| TCATCCAACACATTCTTCAG+CGG | 0.659873 | 4.3:+47414919 | None:intergenic |
| AGAGGTCGACGCAGGCGACG+AGG | 0.660068 | 4.3:+47416555 | None:intergenic |
| GAATTGCTACAGAGCACCGG+TGG | 0.660576 | 4.3:-47410214 | MS.gene66726:CDS |
| TTGCCAGGGCTGAAGACAGA+TGG | 0.664980 | 4.3:-47412485 | MS.gene66726:CDS |
| GTTTCTTGGTCATCACTACG+AGG | 0.684593 | 4.3:-47407053 | MS.gene66726:CDS |
| TTTCTTGGTCATCACTACGA+GGG | 0.687354 | 4.3:-47407052 | MS.gene66726:intron |
| AGATATCGTATTGGATAAGG+CGG | 0.692129 | 4.3:+47416504 | None:intergenic |
| GCGGTAAACATCGACGACGG+TGG | 0.697144 | 4.3:+47416523 | None:intergenic |
| ATATGATGCCACTATCGCAA+TGG | 0.698292 | 4.3:+47412442 | None:intergenic |
| GTACGAGAGAGATCAGCACT+CGG | 0.701059 | 4.3:+47416416 | None:intergenic |
| GACATGTTGAGTTCACGAAG+AGG | 0.701734 | 4.3:+47416380 | None:intergenic |
| TCAGTGGGAAGCGATAGCAA+TGG | 0.705355 | 4.3:-47412307 | MS.gene66726:CDS |
| CGAAATGAAGAGAAGCAGCG+TGG | 0.709640 | 4.3:+47416441 | None:intergenic |
| GTGCCGTAGAGAGTTCAGAG+AGG | 0.752779 | 4.3:+47410239 | None:intergenic |
| AAGGCGGTAAACATCGACGA+CGG | 0.772440 | 4.3:+47416520 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 47406900 | 47416648 | 47406900 | ID=MS.gene66726 |
| chr4.3 | mRNA | 47406900 | 47416648 | 47406900 | ID=MS.gene66726.t1;Parent=MS.gene66726 |
| chr4.3 | exon | 47416369 | 47416648 | 47416369 | ID=MS.gene66726.t1.exon1;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47416369 | 47416648 | 47416369 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47414885 | 47415054 | 47414885 | ID=MS.gene66726.t1.exon2;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47414885 | 47415054 | 47414885 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47413714 | 47413817 | 47413714 | ID=MS.gene66726.t1.exon3;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47413714 | 47413817 | 47413714 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47413424 | 47413490 | 47413424 | ID=MS.gene66726.t1.exon4;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47413424 | 47413490 | 47413424 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47412421 | 47412513 | 47412421 | ID=MS.gene66726.t1.exon5;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47412421 | 47412513 | 47412421 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47412171 | 47412335 | 47412171 | ID=MS.gene66726.t1.exon6;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47412171 | 47412335 | 47412171 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47411506 | 47411646 | 47411506 | ID=MS.gene66726.t1.exon7;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47411506 | 47411646 | 47411506 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47411330 | 47411407 | 47411330 | ID=MS.gene66726.t1.exon8;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47411330 | 47411407 | 47411330 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47410199 | 47410311 | 47410199 | ID=MS.gene66726.t1.exon9;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47410199 | 47410311 | 47410199 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47410063 | 47410123 | 47410063 | ID=MS.gene66726.t1.exon10;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47410063 | 47410123 | 47410063 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47407986 | 47408153 | 47407986 | ID=MS.gene66726.t1.exon11;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47407986 | 47408153 | 47407986 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47407497 | 47407598 | 47407497 | ID=MS.gene66726.t1.exon12;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47407497 | 47407598 | 47407497 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47407053 | 47407142 | 47407053 | ID=MS.gene66726.t1.exon13;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47407053 | 47407142 | 47407053 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| chr4.3 | exon | 47406900 | 47406962 | 47406900 | ID=MS.gene66726.t1.exon14;Parent=MS.gene66726.t1 |
| chr4.3 | CDS | 47406900 | 47406962 | 47406900 | ID=cds.MS.gene66726.t1;Parent=MS.gene66726.t1 |
| Gene Sequence |
| Protein sequence |
