Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66850.t1 | XP_024638296.1 | 97.5 | 157 | 1 | 1 | 1 | 157 | 1 | 154 | 5.60E-79 | 303.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66850.t1 | Q54SW1 | 28.2 | 142 | 89 | 3 | 9 | 144 | 6 | 140 | 1.4e-09 | 64.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66850.t1 | I3SJM8 | 97.5 | 157 | 1 | 1 | 1 | 157 | 1 | 154 | 4.0e-79 | 303.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052673 | MS.gene66850 | 0.807739 | 4.36E-50 | -1.69E-46 |
| MS.gene060634 | MS.gene66850 | 0.812264 | 4.63E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene46429 | MS.gene66850 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66850.t1 | MTR_4g050400 | 92.308 | 130 | 7 | 1 | 1 | 130 | 1 | 127 | 6.63e-69 | 206 |
| MS.gene66850.t1 | MTR_1g055425 | 55.200 | 125 | 56 | 0 | 33 | 157 | 31 | 155 | 2.54e-47 | 151 |
| MS.gene66850.t1 | MTR_1g055435 | 55.200 | 125 | 56 | 0 | 33 | 157 | 32 | 156 | 3.29e-44 | 143 |
| MS.gene66850.t1 | MTR_5g085900 | 43.750 | 128 | 69 | 2 | 24 | 148 | 26 | 153 | 6.70e-34 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66850.t1 | AT3G44100 | 61.871 | 139 | 49 | 2 | 16 | 154 | 15 | 149 | 9.25e-56 | 172 |
| MS.gene66850.t1 | AT3G11780 | 50.000 | 148 | 73 | 1 | 10 | 157 | 7 | 153 | 2.76e-47 | 150 |
| MS.gene66850.t1 | AT5G06480 | 51.938 | 129 | 62 | 0 | 29 | 157 | 25 | 153 | 2.01e-44 | 143 |
| MS.gene66850.t1 | AT3G11780 | 44.578 | 166 | 73 | 2 | 10 | 157 | 7 | 171 | 6.33e-43 | 140 |
Find 46 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGCAGGTAAAGCTCTTTA+TGG | 0.233971 | 4.4:+51970387 | MS.gene66850:intron |
| TAAATTTAATGCAAGTTAAC+TGG | 0.293636 | 4.4:-51977973 | None:intergenic |
| TATGGCGGAGATGTGGTAAT+TGG | 0.323192 | 4.4:+51970405 | MS.gene66850:CDS |
| TAATTGGAGTTTCCTATGTT+GGG | 0.358950 | 4.4:+51970421 | MS.gene66850:CDS |
| TTTAAGATCTCAGCTACTTC+TGG | 0.359491 | 4.4:+51969541 | MS.gene66850:CDS |
| GCAGGGGCACCACTGACAAC+AGG | 0.374041 | 4.4:-51969514 | None:intergenic |
| GGAGAGAAGAAGAAAGAGAT+TGG | 0.382690 | 4.4:-51969219 | None:intergenic |
| ACAAAATTTCCATTAGCAAC+TGG | 0.385673 | 4.4:-51970492 | None:intergenic |
| GTATCCTGTCCAGTTGCTAA+TGG | 0.399485 | 4.4:+51970483 | MS.gene66850:CDS |
| AGAAGTAGCTGAGATCTTAA+AGG | 0.414793 | 4.4:-51969539 | None:intergenic |
| TGAAAGGAAGAGGGTGGAGA+AGG | 0.431347 | 4.4:-51969240 | None:intergenic |
| GTAATTGGAGTTTCCTATGT+TGG | 0.445028 | 4.4:+51970420 | MS.gene66850:CDS |
| GCACCACTGACAACAGGGTT+TGG | 0.451179 | 4.4:-51969508 | None:intergenic |
| GAGTAATTGCAGGTAGTGTC+TGG | 0.460782 | 4.4:-51970527 | None:intergenic |
| TCAATACCAGTCACCTTCAC+AGG | 0.469125 | 4.4:-51969478 | None:intergenic |
| AGTGTCATCTTCAGAGCATA+AGG | 0.472220 | 4.4:-51977932 | None:intergenic |
| ATCTGTGTATGTAGATAAGA+AGG | 0.477363 | 4.4:+51969447 | MS.gene66850:intron |
| TACTTCCTTACACAGATCAA+TGG | 0.478210 | 4.4:-51970463 | None:intergenic |
| GAGAGAAGAAGAAAGAGATT+GGG | 0.492297 | 4.4:-51969218 | None:intergenic |
| TGAAACCATTGATCTGTGTA+AGG | 0.498467 | 4.4:+51970458 | MS.gene66850:CDS |
| CATGAAGAGATGAAAGGAAG+AGG | 0.499717 | 4.4:-51969250 | None:intergenic |
| GATTATCCTGTGAAGGTGAC+TGG | 0.505428 | 4.4:+51969472 | MS.gene66850:CDS |
| GCTGAGATCTTAAAGGTGGC+AGG | 0.517736 | 4.4:-51969532 | None:intergenic |
| ATTTCCATTAGCAACTGGAC+AGG | 0.518555 | 4.4:-51970487 | None:intergenic |
| AAGAGATGAAAGGAAGAGGG+TGG | 0.520636 | 4.4:-51969246 | None:intergenic |
| ACAGATCAATGGTTTCAGAA+TGG | 0.526306 | 4.4:-51970452 | None:intergenic |
| ATGGACAGGCACCCCAACAT+AGG | 0.561157 | 4.4:-51970433 | None:intergenic |
| ATGAAGAGATGAAAGGAAGA+GGG | 0.561296 | 4.4:-51969249 | None:intergenic |
| CTTGAACATGAAGAGATGAA+AGG | 0.572499 | 4.4:-51969256 | None:intergenic |
| CTGAGATCTTAAAGGTGGCA+GGG | 0.584959 | 4.4:-51969531 | None:intergenic |
| TGAGATCTTAAAGGTGGCAG+GGG | 0.586631 | 4.4:-51969530 | None:intergenic |
| AGCTCTTTATGGCGGAGATG+TGG | 0.589630 | 4.4:+51970398 | MS.gene66850:CDS |
| CTAACCGGTGGAGTAATTGC+AGG | 0.597019 | 4.4:-51970537 | None:intergenic |
| AATTGGAGTTTCCTATGTTG+GGG | 0.597198 | 4.4:+51970422 | MS.gene66850:CDS |
| TGCTCTGAAGATGACACTGA+AGG | 0.597832 | 4.4:+51977937 | MS.gene66850:CDS |
| GAAGGCAGATTATCCTGTGA+AGG | 0.607265 | 4.4:+51969465 | MS.gene66850:CDS |
| AAAGTTACTTTCAAATATTG+CGG | 0.619789 | 4.4:+51969281 | MS.gene66850:CDS |
| ACTACCTGCAATTACTCCAC+CGG | 0.625559 | 4.4:+51970533 | MS.gene66850:CDS |
| CAGGGGCACCACTGACAACA+GGG | 0.629803 | 4.4:-51969513 | None:intergenic |
| TCAATGGTTTCAGAATGGAC+AGG | 0.634798 | 4.4:-51970447 | None:intergenic |
| AGTAGCTGAGATCTTAAAGG+TGG | 0.634897 | 4.4:-51969536 | None:intergenic |
| TTACCAAACCCTGTTGTCAG+TGG | 0.649255 | 4.4:+51969505 | MS.gene66850:CDS |
| GCAGGTAAAGCTCTTTATGG+CGG | 0.686450 | 4.4:+51970390 | MS.gene66850:intron |
| GATGACACTGAAGGATGACA+AGG | 0.688205 | 4.4:+51977946 | MS.gene66850:CDS |
| ATGACACTGAAGGATGACAA+GGG | 0.725336 | 4.4:+51977947 | MS.gene66850:CDS |
| ACACTGAAGGATGACAAGGG+TGG | 0.761141 | 4.4:+51977950 | MS.gene66850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 51969200 | 51978036 | 51969200 | ID=MS.gene66850 |
| chr4.4 | mRNA | 51969200 | 51978036 | 51969200 | ID=MS.gene66850.t1;Parent=MS.gene66850 |
| chr4.4 | exon | 51969200 | 51969302 | 51969200 | ID=MS.gene66850.t1.exon1;Parent=MS.gene66850.t1 |
| chr4.4 | CDS | 51969200 | 51969302 | 51969200 | ID=cds.MS.gene66850.t1;Parent=MS.gene66850.t1 |
| chr4.4 | exon | 51969461 | 51969562 | 51969461 | ID=MS.gene66850.t1.exon2;Parent=MS.gene66850.t1 |
| chr4.4 | CDS | 51969461 | 51969562 | 51969461 | ID=cds.MS.gene66850.t1;Parent=MS.gene66850.t1 |
| chr4.4 | exon | 51970394 | 51970554 | 51970394 | ID=MS.gene66850.t1.exon3;Parent=MS.gene66850.t1 |
| chr4.4 | CDS | 51970394 | 51970554 | 51970394 | ID=cds.MS.gene66850.t1;Parent=MS.gene66850.t1 |
| chr4.4 | exon | 51977929 | 51978036 | 51977929 | ID=MS.gene66850.t1.exon4;Parent=MS.gene66850.t1 |
| chr4.4 | CDS | 51977929 | 51978036 | 51977929 | ID=cds.MS.gene66850.t1;Parent=MS.gene66850.t1 |
| Gene Sequence |
| Protein sequence |
