Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66936.t1 | XP_024639431.1 | 94.4 | 71 | 4 | 0 | 1 | 71 | 205 | 275 | 1.00E-25 | 125.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66936.t1 | A0A396HXP9 | 94.4 | 71 | 4 | 0 | 1 | 71 | 205 | 275 | 7.3e-26 | 125.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene66936 | 0.857346 | 1.74E-62 | -1.69E-46 |
| MS.gene05423 | MS.gene66936 | 0.824122 | 9.58E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene66936 | 0.80626 | 8.97E-50 | -1.69E-46 |
| MS.gene059276 | MS.gene66936 | 0.811673 | 6.22E-51 | -1.69E-46 |
| MS.gene059458 | MS.gene66936 | 0.806539 | 7.83E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCTTGGCAGATGTACTAT+TGG | 0.320559 | 5.4:-49920852 | None:intergenic |
| AAAAGGACAAAGAGCATCTA+AGG | 0.339255 | 5.4:+49920610 | MS.gene66936:CDS |
| ATCTGTTGAAAATAGCATAT+TGG | 0.366775 | 5.4:+49920760 | MS.gene66936:CDS |
| GAAAGGAGTCATAGAGAATC+TGG | 0.400717 | 5.4:+49920818 | MS.gene66936:CDS |
| TGAAAATAGCATATTGGAAA+AGG | 0.455763 | 5.4:+49920766 | MS.gene66936:CDS |
| TGAATTGATGTCTCTAGAAA+AGG | 0.480662 | 5.4:+49920593 | MS.gene66936:CDS |
| TAGTGATGAAATACAAGGAA+AGG | 0.521881 | 5.4:+49920801 | MS.gene66936:CDS |
| TAACCTCGACAGAGCTGAAG+AGG | 0.559410 | 5.4:+49920635 | MS.gene66936:CDS |
| TATTGGAAAAGGAGAACAGA+AGG | 0.588297 | 5.4:+49920777 | MS.gene66936:CDS |
| CCTCGACAGAGCTGAAGAGG+AGG | 0.608059 | 5.4:+49920638 | MS.gene66936:CDS |
| TACCAATAGTACATCTGCCA+AGG | 0.612710 | 5.4:+49920850 | MS.gene66936:CDS |
| CCTCCTCTTCAGCTCTGTCG+AGG | 0.622887 | 5.4:-49920638 | None:intergenic |
| AAGGTTAGTGATGAAATACA+AGG | 0.658115 | 5.4:+49920796 | MS.gene66936:CDS |
| GTAGCTTAAAGCAATCAACT+CGG | 0.659694 | 5.4:+49920550 | None:intergenic |
| GGAGTCATAGAGAATCTGGT+GGG | 0.674277 | 5.4:+49920822 | MS.gene66936:CDS |
| AGGAGTCATAGAGAATCTGG+TGG | 0.681121 | 5.4:+49920821 | MS.gene66936:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCAGTTTTTGGATTATAA+TGG | + | chr5.4:49920695-49920714 | MS.gene66936:intron | 20.0% |
| ! | AAAAAAGTCGACAATATAAG+GGG | - | chr5.4:49920678-49920697 | None:intergenic | 25.0% |
| ! | ATCTGTTGAAAATAGCATAT+TGG | + | chr5.4:49920760-49920779 | MS.gene66936:CDS | 25.0% |
| ! | GAAAAAAGTCGACAATATAA+GGG | - | chr5.4:49920679-49920698 | None:intergenic | 25.0% |
| ! | TGAAAAAAGTCGACAATATA+AGG | - | chr5.4:49920680-49920699 | None:intergenic | 25.0% |
| ! | TGAAAATAGCATATTGGAAA+AGG | + | chr5.4:49920766-49920785 | MS.gene66936:CDS | 25.0% |
| AAAAAGTCGACAATATAAGG+GGG | - | chr5.4:49920677-49920696 | None:intergenic | 30.0% | |
| AAGGTTAGTGATGAAATACA+AGG | + | chr5.4:49920796-49920815 | MS.gene66936:CDS | 30.0% | |
| TAGTGATGAAATACAAGGAA+AGG | + | chr5.4:49920801-49920820 | MS.gene66936:CDS | 30.0% | |
| ! | TGAATTGATGTCTCTAGAAA+AGG | + | chr5.4:49920593-49920612 | MS.gene66936:CDS | 30.0% |
| ! | TTATAATGGAATGACTGACT+TGG | + | chr5.4:49920709-49920728 | MS.gene66936:intron | 30.0% |
| !!! | TGTCGACTTTTTTCAGTTTT+TGG | + | chr5.4:49920685-49920704 | MS.gene66936:intron | 30.0% |
| TATTGGAAAAGGAGAACAGA+AGG | + | chr5.4:49920777-49920796 | MS.gene66936:CDS | 35.0% | |
| !! | AAAAGGACAAAGAGCATCTA+AGG | + | chr5.4:49920610-49920629 | MS.gene66936:CDS | 35.0% |
| GAAAGGAGTCATAGAGAATC+TGG | + | chr5.4:49920818-49920837 | MS.gene66936:CDS | 40.0% | |
| !! | TTGGCTTGGTGCAAACTATA+GGG | + | chr5.4:49920728-49920747 | MS.gene66936:intron | 40.0% |
| AGGAGTCATAGAGAATCTGG+TGG | + | chr5.4:49920821-49920840 | MS.gene66936:CDS | 45.0% | |
| GGAGTCATAGAGAATCTGGT+GGG | + | chr5.4:49920822-49920841 | MS.gene66936:CDS | 45.0% | |
| !! | ATGGAATGACTGACTTGGCT+TGG | + | chr5.4:49920714-49920733 | MS.gene66936:intron | 45.0% |
| !! | CTTGGCTTGGTGCAAACTAT+AGG | + | chr5.4:49920727-49920746 | MS.gene66936:intron | 45.0% |
| TAACCTCGACAGAGCTGAAG+AGG | + | chr5.4:49920635-49920654 | MS.gene66936:CDS | 50.0% | |
| ACAGAGCTGAAGAGGAGGTG+AGG | + | chr5.4:49920643-49920662 | MS.gene66936:intron | 55.0% | |
| CCTCCTCTTCAGCTCTGTCG+AGG | - | chr5.4:49920641-49920660 | None:intergenic | 60.0% | |
| CCTCGACAGAGCTGAAGAGG+AGG | + | chr5.4:49920638-49920657 | MS.gene66936:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 49920554 | 49920871 | 49920554 | ID=MS.gene66936 |
| chr5.4 | mRNA | 49920554 | 49920871 | 49920554 | ID=MS.gene66936.t1;Parent=MS.gene66936 |
| chr5.4 | exon | 49920554 | 49920659 | 49920554 | ID=MS.gene66936.t1.exon1;Parent=MS.gene66936.t1 |
| chr5.4 | CDS | 49920554 | 49920659 | 49920554 | ID=cds.MS.gene66936.t1;Parent=MS.gene66936.t1 |
| chr5.4 | exon | 49920749 | 49920871 | 49920749 | ID=MS.gene66936.t1.exon2;Parent=MS.gene66936.t1 |
| chr5.4 | CDS | 49920749 | 49920871 | 49920749 | ID=cds.MS.gene66936.t1;Parent=MS.gene66936.t1 |
| Gene Sequence |
| Protein sequence |
