Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67618.t1 | KEH16652.1 | 88.5 | 130 | 15 | 0 | 1 | 130 | 1 | 130 | 8.60E-36 | 159.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67618.t1 | A0A072TGZ8 | 88.5 | 130 | 15 | 0 | 1 | 130 | 1 | 130 | 6.2e-36 | 159.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05435 | MS.gene67618 | 0.81023 | 1.28E-50 | -1.69E-46 |
| MS.gene055357 | MS.gene67618 | 0.811829 | 5.76E-51 | -1.69E-46 |
| MS.gene058515 | MS.gene67618 | 0.805506 | 1.29E-49 | -1.69E-46 |
| MS.gene059276 | MS.gene67618 | 0.814508 | 1.49E-51 | -1.69E-46 |
| MS.gene059584 | MS.gene67618 | 0.823707 | 1.20E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67618.t1 | MTR_0125s0060 | 89.231 | 130 | 14 | 0 | 1 | 130 | 1 | 130 | 3.19e-79 | 229 |
| MS.gene67618.t1 | MTR_2g035110 | 80.741 | 135 | 26 | 0 | 1 | 135 | 1 | 135 | 1.11e-70 | 208 |
| MS.gene67618.t1 | MTR_8g066845 | 58.491 | 106 | 24 | 3 | 31 | 135 | 1 | 87 | 8.26e-27 | 95.9 |
| MS.gene67618.t1 | MTR_4g012560 | 67.742 | 62 | 15 | 1 | 48 | 109 | 24 | 80 | 2.36e-17 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGACAATGATATTTATA+TGG | 0.247121 | 3.3:-9889001 | MS.gene67618:CDS |
| AACAAGAAGGGGCGCATAAT+TGG | 0.285201 | 3.3:-9888961 | MS.gene67618:CDS |
| TGAATTGAAGTGTTCATATT+AGG | 0.299166 | 3.3:+9888736 | None:intergenic |
| CCATTATCACCTTCTCCTTC+CGG | 0.338322 | 3.3:+9888712 | None:intergenic |
| TTCTACCGATGTAGATTCTT+CGG | 0.370531 | 3.3:-9888884 | MS.gene67618:CDS |
| GAGCGCTTGGAGCAAATGAT+TGG | 0.380489 | 3.3:-9888769 | MS.gene67618:CDS |
| GATATTTATATGGAAATTGT+TGG | 0.391812 | 3.3:-9888991 | MS.gene67618:CDS |
| GTTTCTCAAACGTTTGAGTT+TGG | 0.397323 | 3.3:+9889067 | None:intergenic |
| CCGGAAGGAGAAGGTGATAA+TGG | 0.416904 | 3.3:-9888712 | MS.gene67618:CDS |
| AAGGGGCGCATAATTGGATT+AGG | 0.427709 | 3.3:-9888955 | MS.gene67618:CDS |
| AGCGCTTGGAGCAAATGATT+GGG | 0.510645 | 3.3:-9888768 | MS.gene67618:CDS |
| TGTTGGAGGCATTAACAAGA+AGG | 0.515610 | 3.3:-9888974 | MS.gene67618:CDS |
| AAAACTGAAGATCGAGCGCT+TGG | 0.517015 | 3.3:-9888782 | MS.gene67618:CDS |
| ATTTATATGGAAATTGTTGG+AGG | 0.522832 | 3.3:-9888988 | MS.gene67618:CDS |
| TCATTGTCAATTTCTTCAGT+AGG | 0.525205 | 3.3:+9889012 | None:intergenic |
| TATGAACACTTCAATTCAGC+CGG | 0.533653 | 3.3:-9888731 | MS.gene67618:CDS |
| TCAATTCAGCCGGAAGGAGA+AGG | 0.553755 | 3.3:-9888721 | MS.gene67618:CDS |
| TCTACATCGGTAGAAATTGA+AGG | 0.554924 | 3.3:+9888892 | None:intergenic |
| GTTGGAGGCATTAACAAGAA+GGG | 0.555037 | 3.3:-9888973 | MS.gene67618:CDS |
| TTGGAGGCATTAACAAGAAG+GGG | 0.580609 | 3.3:-9888972 | MS.gene67618:CDS |
| TCGGTAGAAATTGAAGGCGA+TGG | 0.604104 | 3.3:+9888898 | None:intergenic |
| AACACTTCAATTCAGCCGGA+AGG | 0.621409 | 3.3:-9888727 | MS.gene67618:CDS |
| TTTGAGAAACACTCTCAAAG+TGG | 0.627086 | 3.3:-9889054 | MS.gene67618:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGACAATGATATTTATA+TGG | - | chr3.3:9888758-9888777 | MS.gene67618:CDS | 15.0% |
| !! | GATATTTATATGGAAATTGT+TGG | - | chr3.3:9888768-9888787 | MS.gene67618:CDS | 20.0% |
| ! | ATTTATATGGAAATTGTTGG+AGG | - | chr3.3:9888771-9888790 | MS.gene67618:CDS | 25.0% |
| !! | TGAATTGAAGTGTTCATATT+AGG | + | chr3.3:9889026-9889045 | None:intergenic | 25.0% |
| TCATTGTCAATTTCTTCAGT+AGG | + | chr3.3:9888750-9888769 | None:intergenic | 30.0% | |
| ! | TTTTTCCGAAGAATCTACAT+CGG | + | chr3.3:9888883-9888902 | None:intergenic | 30.0% |
| TATGAACACTTCAATTCAGC+CGG | - | chr3.3:9889028-9889047 | MS.gene67618:CDS | 35.0% | |
| TCTACATCGGTAGAAATTGA+AGG | + | chr3.3:9888870-9888889 | None:intergenic | 35.0% | |
| TTCGGAAAAAGTTGTTGCTA+TGG | - | chr3.3:9888893-9888912 | MS.gene67618:CDS | 35.0% | |
| TTCTACCGATGTAGATTCTT+CGG | - | chr3.3:9888875-9888894 | MS.gene67618:CDS | 35.0% | |
| TTTGAGAAACACTCTCAAAG+TGG | - | chr3.3:9888705-9888724 | MS.gene67618:CDS | 35.0% | |
| ! | GTTTCTCAAACGTTTGAGTT+TGG | + | chr3.3:9888695-9888714 | None:intergenic | 35.0% |
| GGAAAAAGTTGTTGCTATGG+AGG | - | chr3.3:9888896-9888915 | MS.gene67618:CDS | 40.0% | |
| !! | GTTGGAGGCATTAACAAGAA+GGG | - | chr3.3:9888786-9888805 | MS.gene67618:CDS | 40.0% |
| !! | TGTTGGAGGCATTAACAAGA+AGG | - | chr3.3:9888785-9888804 | MS.gene67618:CDS | 40.0% |
| !! | TTGGAGGCATTAACAAGAAG+GGG | - | chr3.3:9888787-9888806 | MS.gene67618:CDS | 40.0% |
| AAAACTGAAGATCGAGCGCT+TGG | - | chr3.3:9888977-9888996 | MS.gene67618:CDS | 45.0% | |
| AACACTTCAATTCAGCCGGA+AGG | - | chr3.3:9889032-9889051 | MS.gene67618:CDS | 45.0% | |
| AGCGCTTGGAGCAAATGATT+GGG | - | chr3.3:9888991-9889010 | MS.gene67618:CDS | 45.0% | |
| CCATTATCACCTTCTCCTTC+CGG | + | chr3.3:9889050-9889069 | None:intergenic | 45.0% | |
| TCGGTAGAAATTGAAGGCGA+TGG | + | chr3.3:9888864-9888883 | None:intergenic | 45.0% | |
| ! | AACAAGAAGGGGCGCATAAT+TGG | - | chr3.3:9888798-9888817 | MS.gene67618:CDS | 45.0% |
| ! | AAGGGGCGCATAATTGGATT+AGG | - | chr3.3:9888804-9888823 | MS.gene67618:CDS | 45.0% |
| CCGGAAGGAGAAGGTGATAA+TGG | - | chr3.3:9889047-9889066 | MS.gene67618:CDS | 50.0% | |
| GAGCGCTTGGAGCAAATGAT+TGG | - | chr3.3:9888990-9889009 | MS.gene67618:CDS | 50.0% | |
| TCAATTCAGCCGGAAGGAGA+AGG | - | chr3.3:9889038-9889057 | MS.gene67618:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 9888687 | 9889094 | 9888687 | ID=MS.gene67618 |
| chr3.3 | mRNA | 9888687 | 9889094 | 9888687 | ID=MS.gene67618.t1;Parent=MS.gene67618 |
| chr3.3 | exon | 9888687 | 9889094 | 9888687 | ID=MS.gene67618.t1.exon1;Parent=MS.gene67618.t1 |
| chr3.3 | CDS | 9888687 | 9889094 | 9888687 | ID=cds.MS.gene67618.t1;Parent=MS.gene67618.t1 |
| Gene Sequence |
| Protein sequence |
