AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68144


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68144.t1 MTR_3g117890 98.718 156 2 0 1 156 1 156 3.13e-116 325
MS.gene68144.t1 MTR_4g073040 79.245 159 27 3 1 156 1 156 2.15e-88 254
MS.gene68144.t1 MTR_5g022620 61.491 161 53 3 1 156 1 157 9.79e-66 197
MS.gene68144.t1 MTR_1g030630 53.205 156 64 3 1 156 1 147 1.57e-53 166
MS.gene68144.t1 MTR_8g091420 51.613 155 69 3 2 156 5 153 5.32e-49 155
MS.gene68144.t1 MTR_3g087770 51.923 156 66 4 2 156 5 152 6.21e-49 155
MS.gene68144.t1 MTR_5g025150 47.436 156 71 3 1 156 1 145 1.55e-45 146
MS.gene68144.t1 MTR_6g003990 45.062 162 76 3 1 156 1 155 6.60e-45 145
MS.gene68144.t1 MTR_7g083060 48.077 156 71 3 1 156 1 146 7.60e-45 144
MS.gene68144.t1 MTR_3g073670 47.742 155 71 3 2 156 5 149 2.79e-42 138
MS.gene68144.t1 MTR_6g003990 46.341 123 61 1 34 156 1 118 3.75e-36 121
MS.gene68144.t1 MTR_5g055020 37.821 156 88 3 1 156 1 147 1.18e-34 118
MS.gene68144.t1 MTR_1g078090 38.686 137 76 4 25 156 52 185 2.78e-24 93.2
MS.gene68144.t1 MTR_6g026890 36.029 136 81 4 25 156 15 148 3.96e-23 89.4
MS.gene68144.t1 MTR_2g091235 36.429 140 77 3 28 156 4 142 4.13e-22 86.3
MS.gene68144.t1 MTR_6g045203 36.364 143 70 6 28 156 5 140 4.91e-18 75.9
MS.gene68144.t1 MTR_7g013660 48.485 66 33 1 26 91 4 68 3.82e-14 65.5
MS.gene68144.t1 MTR_3g435930 34.586 133 74 3 26 156 116 237 4.13e-14 67.4
MS.gene68144.t1 MTR_1g083310 36.765 68 43 0 25 92 14 81 2.49e-12 63.5
MS.gene68144.t1 MTR_1g092670 45.455 66 35 1 26 91 4 68 4.46e-12 58.9
MS.gene68144.t1 MTR_7g108560 36.765 68 42 1 24 91 20 86 6.94e-12 62.0
MS.gene68144.t1 MTR_7g113110 42.857 63 35 1 30 92 37 98 9.96e-12 62.0
MS.gene68144.t1 MTR_3g010130 55.769 52 21 2 1 52 1 50 2.47e-11 56.6
MS.gene68144.t1 MTR_1g107565 39.706 68 40 1 24 91 11 77 3.63e-11 60.5
MS.gene68144.t1 MTR_3g067750 41.538 65 37 1 26 90 12 75 4.83e-11 58.9
MS.gene68144.t1 MTR_7g100450 46.269 67 34 1 26 92 209 273 8.33e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68144.t1 AT4G38580 76.282 156 34 1 1 156 1 153 1.07e-84 245
MS.gene68144.t1 AT4G38580 76.282 156 34 1 1 156 1 153 1.07e-84 245
MS.gene68144.t1 AT4G35060 70.701 157 41 3 1 156 1 153 1.82e-76 224
MS.gene68144.t1 AT5G66110 66.667 138 44 1 19 156 12 147 2.10e-66 199
MS.gene68144.t1 AT5G66110 66.667 138 44 1 19 156 12 147 2.10e-66 199
MS.gene68144.t1 AT5G66110 65.854 123 40 1 34 156 1 121 1.43e-58 178
MS.gene68144.t1 AT1G71050 56.329 158 61 4 1 156 1 152 9.28e-54 167
MS.gene68144.t1 AT1G22990 49.057 159 71 2 1 156 1 152 1.93e-50 159
MS.gene68144.t1 AT1G22990 46.795 156 74 2 1 156 1 147 2.26e-47 150
MS.gene68144.t1 AT4G39700 48.734 158 74 4 2 156 5 158 1.20e-44 144
MS.gene68144.t1 AT5G17450 45.860 157 76 3 1 156 1 149 1.42e-42 139
MS.gene68144.t1 AT4G08570 44.231 156 76 4 2 156 5 150 3.17e-40 132
MS.gene68144.t1 AT5G17450 43.396 106 51 3 52 156 8 105 1.46e-25 94.4
MS.gene68144.t1 AT1G06330 38.000 150 71 6 27 156 3 150 4.74e-24 91.7
MS.gene68144.t1 AT2G18196 33.824 136 84 4 25 156 45 178 3.93e-20 82.4
MS.gene68144.t1 AT4G10465 33.566 143 89 3 18 156 43 183 1.75e-19 80.9
MS.gene68144.t1 AT3G56891 32.544 169 89 6 10 156 1 166 1.52e-18 77.8
MS.gene68144.t1 AT2G18196 33.071 127 79 4 34 156 1 125 2.30e-16 71.2
MS.gene68144.t1 AT3G56891 33.103 145 73 5 34 156 1 143 3.59e-16 71.2
MS.gene68144.t1 AT1G29100 33.974 156 65 4 27 156 4 147 1.23e-15 69.7
MS.gene68144.t1 AT1G29100 33.333 153 64 4 30 156 1 141 3.22e-14 65.9
MS.gene68144.t1 AT1G56210 39.744 78 46 1 25 102 39 115 8.82e-13 65.1
MS.gene68144.t1 AT1G66240 43.939 66 36 1 26 91 33 97 1.17e-12 60.8
MS.gene68144.t1 AT3G56240 47.761 67 34 1 26 92 3 68 2.13e-12 60.8
MS.gene68144.t1 AT3G56240 47.761 67 34 1 26 92 3 68 2.13e-12 60.8
MS.gene68144.t1 AT3G02960 28.571 133 85 2 24 156 124 246 4.41e-12 62.4
MS.gene68144.t1 AT5G27690 39.130 69 41 1 24 92 28 95 1.70e-11 61.2
MS.gene68144.t1 AT3G06130 39.706 68 40 1 24 91 8 74 4.28e-11 60.1

Find 47 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACACCGCAAACAATTTC+AGG 0.262177 3.1:-92733868 MS.gene68144:intron
AGCACACAAGGTCACTGTTC+AGG 0.319616 3.1:-92733663 MS.gene68144:CDS
TCGTACGTACCTTGTGCATA+TGG 0.340646 3.1:+92733542 None:intergenic
TACGACAACAATCAGTATTC+TGG 0.342642 3.1:-92733485 MS.gene68144:CDS
ATACGCGCCACCACTTTGTT+AGG 0.355486 3.1:+92733629 None:intergenic
TCGCTCACCGCACTGGTAAG+AGG 0.420324 3.1:-92733607 MS.gene68144:CDS
CTTTCCTATCAATATCAACT+TGG 0.425944 3.1:+92733684 None:intergenic
GTAGTTGTTTCTAACATAAC+CGG 0.435319 3.1:+92733505 None:intergenic
AAGAGATAATATGGGTGCTC+TGG 0.436844 3.1:-92733934 None:intergenic
GGGTGTGCAACGACGTCGTA+TGG 0.444075 3.1:+92733563 None:intergenic
CTATTACCTGAAATTGTTTG+CGG 0.461292 3.1:+92733862 None:intergenic
GAAATTGTTTGCGGTGTTTG+TGG 0.465422 3.1:+92733871 None:intergenic
ACGACGTCGTATGGAACATA+TGG 0.471597 3.1:+92733572 None:intergenic
TCAGTATTCTGGATCCCATC+TGG 0.473879 3.1:-92733474 MS.gene68144:CDS
TGAACCAAGTTGATATTGAT+AGG 0.475748 3.1:-92733688 MS.gene68144:CDS
AAATTGTTTGCGGTGTTTGT+GGG 0.476948 3.1:+92733872 None:intergenic
GCACACAAGGTCACTGTTCA+GGG 0.478112 3.1:-92733662 MS.gene68144:CDS
TACGACAAGAAAGCACCTTC+CGG 0.490150 3.1:-92733524 MS.gene68144:CDS
ATGATATCACATGACAGAAC+AGG 0.494670 3.1:+92733391 None:intergenic
CTGGTAAGAGGGCTGAGATA+TGG 0.502117 3.1:-92733595 MS.gene68144:CDS
CGTACGTACCTTGTGCATAT+GGG 0.507821 3.1:+92733543 None:intergenic
GTGTTTGTGGGAACTTCCAC+CGG 0.512215 3.1:+92733884 None:intergenic
TGGAACTAGCACGCGCCAGA+TGG 0.513296 3.1:+92733459 None:intergenic
GCGCGTATCGCTCACCGCAC+TGG 0.514553 3.1:-92733614 MS.gene68144:CDS
CGCTCACCGCACTGGTAAGA+GGG 0.517783 3.1:-92733606 MS.gene68144:CDS
TCTGAGCTATTTGACTGCTC+CGG 0.534149 3.1:-92733903 MS.gene68144:CDS
AAAGTGAGAAGGTCGGTGGA+AGG 0.544215 3.1:-92733722 MS.gene68144:CDS
GGTGGAAGTGAAAGTGAAGA+TGG 0.547212 3.1:-92733765 MS.gene68144:CDS
GAGCTATTTGACTGCTCCGG+TGG 0.554467 3.1:-92733900 MS.gene68144:CDS
GGAACTAGCACGCGCCAGAT+GGG 0.557432 3.1:+92733460 None:intergenic
AGGTCGGTGGAAGGAATGAA+AGG 0.586836 3.1:-92733713 MS.gene68144:CDS
GAGAAAAGTGAGAAGGTCGG+TGG 0.602080 3.1:-92733726 MS.gene68144:CDS
AGCGGTGGTGTAGCGAACTT+CGG 0.605621 3.1:+92733436 None:intergenic
TCACATGACAGAACAGGCGG+TGG 0.625825 3.1:+92733397 None:intergenic
CGAGAGAAAAGTGAGAAGGT+CGG 0.626298 3.1:-92733729 MS.gene68144:CDS
TATTGATAGGAAAGCACACA+AGG 0.639331 3.1:-92733675 MS.gene68144:CDS
GATGCGAGAGAAAAGTGAGA+AGG 0.650873 3.1:-92733733 MS.gene68144:CDS
CACATGACAGAACAGGCGGT+GGG 0.664346 3.1:+92733398 None:intergenic
TTGTTTCTAACATAACCGGA+AGG 0.675813 3.1:+92733509 None:intergenic
TGTTGAACCTAACAAAGTGG+TGG 0.684270 3.1:-92733636 MS.gene68144:CDS
CACACAAGGTCACTGTTCAG+GGG 0.691299 3.1:-92733661 MS.gene68144:CDS
GGTGGTGTAGCGAACTTCGG+TGG 0.699721 3.1:+92733439 None:intergenic
ATATCACATGACAGAACAGG+CGG 0.726418 3.1:+92733394 None:intergenic
GTTGCACACCCATATGCACA+AGG 0.727359 3.1:-92733551 MS.gene68144:CDS
GTATGTTGAACCTAACAAAG+TGG 0.728726 3.1:-92733639 MS.gene68144:CDS
TCTCAGCCCTCTTACCAGTG+CGG 0.734386 3.1:+92733600 None:intergenic
AAAGTGAAGATGGACTGCGA+AGG 0.769260 3.1:-92733755 MS.gene68144:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATGTACAGA+CGG - chr3.1:92733535-92733554 MS.gene68144:CDS 15.0%
!! TAACAACATATGAATATGAA+TGG + chr3.1:92733497-92733516 None:intergenic 20.0%
! ACATATGAATATGAATGGAA+TGG + chr3.1:92733492-92733511 None:intergenic 25.0%
ATATATATATGTACAGACGG+TGG - chr3.1:92733538-92733557 MS.gene68144:CDS 30.0%
CTATTACCTGAAATTGTTTG+CGG + chr3.1:92733462-92733481 None:intergenic 30.0%
CTTTCCTATCAATATCAACT+TGG + chr3.1:92733640-92733659 None:intergenic 30.0%
TGAATATGAATGGAATGGAA+TGG + chr3.1:92733487-92733506 None:intergenic 30.0%
!! GTAGTTGTTTCTAACATAAC+CGG + chr3.1:92733819-92733838 None:intergenic 30.0%
!! TGAACCAAGTTGATATTGAT+AGG - chr3.1:92733633-92733652 MS.gene68144:CDS 30.0%
AAATTGTTTGCGGTGTTTGT+GGG + chr3.1:92733452-92733471 None:intergenic 35.0%
GTATGTTGAACCTAACAAAG+TGG - chr3.1:92733682-92733701 MS.gene68144:CDS 35.0%
TACGACAACAATCAGTATTC+TGG - chr3.1:92733836-92733855 MS.gene68144:intron 35.0%
! TATTGATAGGAAAGCACACA+AGG - chr3.1:92733646-92733665 MS.gene68144:CDS 35.0%
!! TTGTTTCTAACATAACCGGA+AGG + chr3.1:92733815-92733834 None:intergenic 35.0%
CAAACACCGCAAACAATTTC+AGG - chr3.1:92733453-92733472 MS.gene68144:CDS 40.0%
GAAATTGTTTGCGGTGTTTG+TGG + chr3.1:92733453-92733472 None:intergenic 40.0%
TGTTGAACCTAACAAAGTGG+TGG - chr3.1:92733685-92733704 MS.gene68144:CDS 40.0%
ACGACGTCGTATGGAACATA+TGG + chr3.1:92733752-92733771 None:intergenic 45.0%
CGAGAGAAAAGTGAGAAGGT+CGG - chr3.1:92733592-92733611 MS.gene68144:CDS 45.0%
CGTACGTACCTTGTGCATAT+GGG + chr3.1:92733781-92733800 None:intergenic 45.0%
GATGCGAGAGAAAAGTGAGA+AGG - chr3.1:92733588-92733607 MS.gene68144:CDS 45.0%
GGTGGAAGTGAAAGTGAAGA+TGG - chr3.1:92733556-92733575 MS.gene68144:CDS 45.0%
TCAGTATTCTGGATCCCATC+TGG - chr3.1:92733847-92733866 MS.gene68144:intron 45.0%
TCGTACGTACCTTGTGCATA+TGG + chr3.1:92733782-92733801 None:intergenic 45.0%
TCTGAGCTATTTGACTGCTC+CGG - chr3.1:92733418-92733437 MS.gene68144:CDS 45.0%
!! AAAGTGAAGATGGACTGCGA+AGG - chr3.1:92733566-92733585 MS.gene68144:CDS 45.0%
!! TACGACAAGAAAGCACCTTC+CGG - chr3.1:92733797-92733816 MS.gene68144:intron 45.0%
AAAGTGAGAAGGTCGGTGGA+AGG - chr3.1:92733599-92733618 MS.gene68144:CDS 50.0%
AGCACACAAGGTCACTGTTC+AGG - chr3.1:92733658-92733677 MS.gene68144:CDS 50.0%
AGGTCGGTGGAAGGAATGAA+AGG - chr3.1:92733608-92733627 MS.gene68144:CDS 50.0%
ATACGCGCCACCACTTTGTT+AGG + chr3.1:92733695-92733714 None:intergenic 50.0%
CACACAAGGTCACTGTTCAG+GGG - chr3.1:92733660-92733679 MS.gene68144:CDS 50.0%
CTGGTAAGAGGGCTGAGATA+TGG - chr3.1:92733726-92733745 MS.gene68144:CDS 50.0%
GAGAAAAGTGAGAAGGTCGG+TGG - chr3.1:92733595-92733614 MS.gene68144:CDS 50.0%
GCACACAAGGTCACTGTTCA+GGG - chr3.1:92733659-92733678 MS.gene68144:CDS 50.0%
GTGTTTGTGGGAACTTCCAC+CGG + chr3.1:92733440-92733459 None:intergenic 50.0%
GTTGCACACCCATATGCACA+AGG - chr3.1:92733770-92733789 MS.gene68144:CDS 50.0%
! ATTTTCGTCGCTGAAAGCGG+TGG + chr3.1:92733903-92733922 None:intergenic 50.0%
! GGGATTTTCGTCGCTGAAAG+CGG + chr3.1:92733906-92733925 None:intergenic 50.0%
AGCGGTGGTGTAGCGAACTT+CGG + chr3.1:92733888-92733907 None:intergenic 55.0%
CACATGACAGAACAGGCGGT+GGG + chr3.1:92733926-92733945 None:intergenic 55.0%
GAGCTATTTGACTGCTCCGG+TGG - chr3.1:92733421-92733440 MS.gene68144:CDS 55.0%
TCACATGACAGAACAGGCGG+TGG + chr3.1:92733927-92733946 None:intergenic 55.0%
TCTCAGCCCTCTTACCAGTG+CGG + chr3.1:92733724-92733743 None:intergenic 55.0%
GGAACTAGCACGCGCCAGAT+GGG + chr3.1:92733864-92733883 None:intergenic 60.0%
GGGTGTGCAACGACGTCGTA+TGG + chr3.1:92733761-92733780 None:intergenic 60.0%
GGTGGTGTAGCGAACTTCGG+TGG + chr3.1:92733885-92733904 None:intergenic 60.0%
TGGAACTAGCACGCGCCAGA+TGG + chr3.1:92733865-92733884 None:intergenic 60.0%
! CGCTCACCGCACTGGTAAGA+GGG - chr3.1:92733715-92733734 MS.gene68144:CDS 60.0%
! TCGCTCACCGCACTGGTAAG+AGG - chr3.1:92733714-92733733 MS.gene68144:CDS 60.0%
GCGCGTATCGCTCACCGCAC+TGG - chr3.1:92733707-92733726 MS.gene68144:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 92733397 92733946 92733397 ID=MS.gene68144
chr3.1 mRNA 92733397 92733946 92733397 ID=MS.gene68144.t1;Parent=MS.gene68144
chr3.1 exon 92733869 92733946 92733869 ID=MS.gene68144.t1.exon1;Parent=MS.gene68144.t1
chr3.1 CDS 92733869 92733946 92733869 ID=cds.MS.gene68144.t1;Parent=MS.gene68144.t1
chr3.1 exon 92733397 92733789 92733397 ID=MS.gene68144.t1.exon2;Parent=MS.gene68144.t1
chr3.1 CDS 92733397 92733789 92733397 ID=cds.MS.gene68144.t1;Parent=MS.gene68144.t1
Gene Sequence

>MS.gene68144

ATGGGTGCTCTGGATCACATTTCTGAGCTATTTGACTGCTCCGGTGGAAGTTCCCACAAACACCGCAAACAATTTCAGGTAATAGATCCATTCCATTCCATTCATATTCATATGTTGTTATTATTATTATTATTAAAATATATATATATATGTACAGACGGTGGAAGTGAAAGTGAAGATGGACTGCGAAGGATGCGAGAGAAAAGTGAGAAGGTCGGTGGAAGGAATGAAAGGTGTGAACCAAGTTGATATTGATAGGAAAGCACACAAGGTCACTGTTCAGGGGTATGTTGAACCTAACAAAGTGGTGGCGCGTATCGCTCACCGCACTGGTAAGAGGGCTGAGATATGGCCATATGTTCCATACGACGTCGTTGCACACCCATATGCACAAGGTACGTACGACAAGAAAGCACCTTCCGGTTATGTTAGAAACAACTACGACAACAATCAGTATTCTGGATCCCATCTGGCGCGTGCTAGTTCCACCGAAGTTCGCTACACCACCGCTTTCAGCGACGAAAATCCCACCGCCTGTTCTGTCATGTGA

Protein sequence

>MS.gene68144.t1

MGALDHISELFDCSGGSSHKHRKQFQTVEVKVKMDCEGCERKVRRSVEGMKGVNQVDIDRKAHKVTVQGYVEPNKVVARIAHRTGKRAEIWPYVPYDVVAHPYAQGTYDKKAPSGYVRNNYDNNQYSGSHLARASSTEVRYTTAFSDENPTACSVM