Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68228.t1 | XP_003603902.1 | 97.6 | 166 | 3 | 1 | 1 | 166 | 121 | 285 | 5.90E-87 | 330.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68228.t1 | O65009 | 43.2 | 162 | 86 | 3 | 1 | 157 | 122 | 282 | 6.5e-34 | 145.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68228.t1 | G7J6J4 | 97.6 | 166 | 3 | 1 | 1 | 166 | 121 | 285 | 4.3e-87 | 330.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050297 | MS.gene68228 | 0.808223 | 3.44E-50 | -1.69E-46 |
| MS.gene052143 | MS.gene68228 | 0.803946 | 2.73E-49 | -1.69E-46 |
| MS.gene056870 | MS.gene68228 | 0.802243 | 6.15E-49 | -1.69E-46 |
| MS.gene059300 | MS.gene68228 | 0.800511 | 1.39E-48 | -1.69E-46 |
| MS.gene060494 | MS.gene68228 | 0.80218 | 6.34E-49 | -1.69E-46 |
| MS.gene06057 | MS.gene68228 | 0.814793 | 1.29E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68228.t1 | MTR_3g116410 | 97.590 | 166 | 3 | 1 | 1 | 166 | 121 | 285 | 6.07e-118 | 335 |
| MS.gene68228.t1 | MTR_3g107830 | 84.940 | 166 | 24 | 1 | 1 | 166 | 101 | 265 | 2.67e-104 | 300 |
| MS.gene68228.t1 | MTR_4g069680 | 77.108 | 166 | 37 | 1 | 1 | 166 | 132 | 296 | 1.69e-93 | 273 |
| MS.gene68228.t1 | MTR_3g116380 | 51.282 | 156 | 74 | 2 | 1 | 156 | 149 | 302 | 3.79e-57 | 181 |
| MS.gene68228.t1 | MTR_3g116270 | 50.000 | 156 | 77 | 1 | 1 | 156 | 351 | 505 | 1.02e-52 | 175 |
| MS.gene68228.t1 | MTR_6g086530 | 47.799 | 159 | 81 | 2 | 1 | 157 | 48 | 206 | 4.54e-51 | 162 |
| MS.gene68228.t1 | MTR_3g116320 | 48.718 | 156 | 79 | 1 | 1 | 156 | 533 | 687 | 2.97e-50 | 171 |
| MS.gene68228.t1 | MTR_4g069570 | 46.835 | 158 | 81 | 3 | 1 | 156 | 179 | 335 | 6.41e-49 | 161 |
| MS.gene68228.t1 | MTR_4g069550 | 48.485 | 165 | 82 | 3 | 1 | 163 | 306 | 469 | 1.27e-48 | 163 |
| MS.gene68228.t1 | MTR_4g069040 | 45.122 | 164 | 87 | 3 | 1 | 162 | 353 | 515 | 2.34e-48 | 163 |
| MS.gene68228.t1 | MTR_4g069520 | 45.455 | 165 | 87 | 3 | 1 | 163 | 566 | 729 | 5.23e-44 | 154 |
| MS.gene68228.t1 | MTR_4g069520 | 45.455 | 165 | 87 | 3 | 1 | 163 | 552 | 715 | 6.64e-44 | 154 |
| MS.gene68228.t1 | MTR_3g109490 | 45.625 | 160 | 80 | 5 | 1 | 157 | 167 | 322 | 2.69e-43 | 146 |
| MS.gene68228.t1 | MTR_1g008500 | 47.468 | 158 | 77 | 5 | 1 | 157 | 233 | 385 | 1.25e-41 | 143 |
| MS.gene68228.t1 | MTR_1g008530 | 44.304 | 158 | 82 | 5 | 1 | 157 | 72 | 224 | 1.65e-39 | 134 |
| MS.gene68228.t1 | MTR_0312s0030 | 44.304 | 158 | 81 | 5 | 1 | 157 | 174 | 325 | 1.41e-38 | 134 |
| MS.gene68228.t1 | MTR_1g007520 | 44.304 | 158 | 81 | 5 | 1 | 157 | 174 | 325 | 1.41e-38 | 134 |
| MS.gene68228.t1 | MTR_4g068420 | 44.521 | 146 | 60 | 3 | 20 | 163 | 1 | 127 | 1.71e-38 | 128 |
| MS.gene68228.t1 | MTR_1g008590 | 44.304 | 158 | 81 | 5 | 1 | 157 | 174 | 325 | 2.01e-38 | 133 |
| MS.gene68228.t1 | MTR_1g008580 | 43.590 | 156 | 82 | 5 | 1 | 155 | 20 | 170 | 4.21e-38 | 128 |
| MS.gene68228.t1 | MTR_1g008560 | 43.671 | 158 | 83 | 5 | 1 | 157 | 178 | 330 | 7.23e-37 | 129 |
| MS.gene68228.t1 | MTR_1g008510 | 43.671 | 158 | 83 | 5 | 1 | 157 | 174 | 326 | 1.18e-36 | 129 |
| MS.gene68228.t1 | MTR_1g008470 | 41.401 | 157 | 86 | 4 | 1 | 157 | 150 | 300 | 1.96e-36 | 127 |
| MS.gene68228.t1 | MTR_1g008450 | 40.127 | 157 | 88 | 3 | 1 | 157 | 163 | 313 | 1.22e-33 | 120 |
| MS.gene68228.t1 | MTR_1g008460 | 40.764 | 157 | 87 | 4 | 1 | 157 | 147 | 297 | 1.65e-33 | 120 |
| MS.gene68228.t1 | MTR_5g034320 | 35.000 | 160 | 91 | 6 | 1 | 155 | 476 | 627 | 4.09e-24 | 98.2 |
| MS.gene68228.t1 | MTR_8g064500 | 34.615 | 156 | 89 | 6 | 4 | 154 | 223 | 370 | 4.65e-24 | 96.7 |
| MS.gene68228.t1 | MTR_4g133840 | 32.468 | 154 | 98 | 5 | 3 | 154 | 37 | 186 | 4.45e-23 | 90.5 |
| MS.gene68228.t1 | MTR_8g064560 | 33.758 | 157 | 91 | 6 | 4 | 155 | 476 | 624 | 7.79e-22 | 91.7 |
| MS.gene68228.t1 | MTR_8g046000 | 37.908 | 153 | 85 | 5 | 4 | 156 | 80 | 222 | 6.26e-21 | 85.9 |
| MS.gene68228.t1 | MTR_3g078090 | 33.548 | 155 | 96 | 5 | 3 | 155 | 476 | 625 | 2.16e-20 | 87.4 |
| MS.gene68228.t1 | MTR_8g445140 | 37.500 | 144 | 74 | 5 | 16 | 156 | 172 | 302 | 1.42e-18 | 80.9 |
| MS.gene68228.t1 | MTR_8g064530 | 31.013 | 158 | 98 | 4 | 1 | 154 | 490 | 640 | 1.48e-18 | 82.0 |
| MS.gene68228.t1 | MTR_2g081590 | 34.437 | 151 | 89 | 5 | 8 | 158 | 121 | 261 | 3.70e-18 | 79.3 |
| MS.gene68228.t1 | MTR_2g081610 | 33.775 | 151 | 90 | 5 | 8 | 158 | 121 | 261 | 1.23e-17 | 77.8 |
| MS.gene68228.t1 | MTR_8g445170 | 34.043 | 141 | 83 | 4 | 16 | 156 | 253 | 383 | 2.19e-16 | 75.5 |
| MS.gene68228.t1 | MTR_8g045890 | 34.043 | 141 | 83 | 4 | 16 | 156 | 342 | 472 | 2.57e-16 | 75.5 |
| MS.gene68228.t1 | MTR_8g045890 | 34.043 | 141 | 83 | 4 | 16 | 156 | 301 | 431 | 2.61e-16 | 75.5 |
| MS.gene68228.t1 | MTR_8g045890 | 34.043 | 141 | 83 | 4 | 16 | 156 | 379 | 509 | 2.62e-16 | 75.5 |
| MS.gene68228.t1 | MTR_5g033350 | 29.032 | 155 | 104 | 4 | 1 | 154 | 49 | 198 | 1.75e-15 | 70.9 |
| MS.gene68228.t1 | MTR_8g045880 | 33.333 | 141 | 84 | 4 | 16 | 156 | 184 | 314 | 6.21e-15 | 71.2 |
| MS.gene68228.t1 | MTR_5g033340 | 29.032 | 155 | 104 | 4 | 1 | 154 | 228 | 377 | 8.61e-15 | 70.9 |
| MS.gene68228.t1 | MTR_8g045880 | 33.333 | 141 | 84 | 4 | 16 | 156 | 219 | 349 | 8.81e-15 | 70.9 |
| MS.gene68228.t1 | MTR_8g045880 | 33.333 | 141 | 84 | 4 | 16 | 156 | 221 | 351 | 9.42e-15 | 70.9 |
| MS.gene68228.t1 | MTR_8g075930 | 44.737 | 76 | 37 | 4 | 73 | 145 | 14 | 87 | 2.22e-14 | 65.1 |
| MS.gene68228.t1 | MTR_1g045860 | 30.769 | 156 | 99 | 5 | 2 | 157 | 114 | 260 | 2.08e-13 | 66.2 |
| MS.gene68228.t1 | MTR_5g033330 | 28.387 | 155 | 105 | 4 | 1 | 154 | 467 | 616 | 4.61e-13 | 66.2 |
| MS.gene68228.t1 | MTR_5g033330 | 28.777 | 139 | 93 | 4 | 1 | 138 | 51 | 184 | 8.08e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68228.t1 | AT1G49320 | 42.593 | 162 | 87 | 4 | 1 | 157 | 120 | 280 | 6.58e-43 | 144 |
| MS.gene68228.t1 | AT5G25610 | 43.871 | 155 | 82 | 5 | 1 | 154 | 239 | 389 | 1.09e-35 | 128 |
| MS.gene68228.t1 | AT1G70370 | 34.211 | 152 | 93 | 4 | 5 | 154 | 476 | 622 | 4.80e-20 | 86.7 |
| MS.gene68228.t1 | AT1G70370 | 34.211 | 152 | 93 | 4 | 5 | 154 | 476 | 622 | 4.80e-20 | 86.7 |
| MS.gene68228.t1 | AT1G60390 | 32.692 | 156 | 98 | 5 | 1 | 154 | 470 | 620 | 1.14e-19 | 85.5 |
| MS.gene68228.t1 | AT1G23760 | 32.237 | 152 | 96 | 5 | 5 | 154 | 472 | 618 | 1.37e-18 | 82.4 |
Find 31 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACCTTGTGATTTCAATTT+AGG | 0.099086 | 3.4:+99226161 | None:intergenic |
| CATGTTTCATTTCAAGTTCT+TGG | 0.201295 | 3.4:-99226245 | MS.gene68228:CDS |
| AACCTTGTGATTTCAATTTA+GGG | 0.289344 | 3.4:+99226162 | None:intergenic |
| TTGAAAAGTAACACTTGATT+TGG | 0.297099 | 3.4:+99226475 | None:intergenic |
| GAATTTACACAAAACATTCT+TGG | 0.298406 | 3.4:-99226539 | MS.gene68228:CDS |
| GAACTTAACCTCTCCTTTGA+TGG | 0.356006 | 3.4:+99226589 | None:intergenic |
| GTGTGACAAGCCACCATCTT+TGG | 0.368463 | 3.4:+99226413 | None:intergenic |
| TCCCAGCGGACAATTACATT+TGG | 0.373004 | 3.4:-99226187 | MS.gene68228:CDS |
| CCCAGCGGACAATTACATTT+GGG | 0.389028 | 3.4:-99226186 | MS.gene68228:CDS |
| GGCTATGGTGGTTTGTCACT+TGG | 0.403171 | 3.4:-99226291 | MS.gene68228:CDS |
| ATAGTCAAGAAAGTGAGAAT+AGG | 0.435139 | 3.4:-99226358 | MS.gene68228:CDS |
| TGGAGATAAGGTTGAGGCTA+TGG | 0.442985 | 3.4:-99226306 | MS.gene68228:CDS |
| TGACACACAGAAGAGCTTCC+TGG | 0.469514 | 3.4:+99226215 | None:intergenic |
| TGTGCAAAGTTGCATAAGCT+TGG | 0.472166 | 3.4:+99226501 | None:intergenic |
| CAAGTTCTTGGTGTCACACC+AGG | 0.473983 | 3.4:-99226233 | MS.gene68228:CDS |
| CCCAAATGTAATTGTCCGCT+GGG | 0.478272 | 3.4:+99226186 | None:intergenic |
| TAGTCAAGAAAGTGAGAATA+GGG | 0.482578 | 3.4:-99226357 | MS.gene68228:CDS |
| TCACTTGGATACATCACAAT+GGG | 0.486976 | 3.4:-99226276 | MS.gene68228:CDS |
| ACCCAAATGTAATTGTCCGC+TGG | 0.506688 | 3.4:+99226185 | None:intergenic |
| AACTTAACCTCTCCTTTGAT+GGG | 0.514268 | 3.4:+99226590 | None:intergenic |
| CACTATAGTAGAAATTATGA+TGG | 0.530352 | 3.4:-99226447 | MS.gene68228:CDS |
| AGATAAGGTTGAGGCTATGG+TGG | 0.538336 | 3.4:-99226303 | MS.gene68228:CDS |
| TTCAAGCATAGATTCTAAAG+AGG | 0.545516 | 3.4:+99226559 | None:intergenic |
| GGAAATTCTAGCTCCAAAGA+TGG | 0.597283 | 3.4:-99226426 | MS.gene68228:CDS |
| GAAAGCAGCAAACCCATCAA+AGG | 0.600510 | 3.4:-99226602 | MS.gene68228:CDS |
| GTCCCTAAATTGAAATCACA+AGG | 0.602399 | 3.4:-99226164 | MS.gene68228:CDS |
| TGAGAATGGAGATAAGGTTG+AGG | 0.616111 | 3.4:-99226312 | MS.gene68228:CDS |
| GTCACTTGGATACATCACAA+TGG | 0.635200 | 3.4:-99226277 | MS.gene68228:CDS |
| CAGCAAACCCATCAAAGGAG+AGG | 0.643987 | 3.4:-99226597 | MS.gene68228:CDS |
| ACTTGAAATGAAACATGACT+AGG | 0.655390 | 3.4:+99226251 | None:intergenic |
| AATTCTAGCTCCAAAGATGG+TGG | 0.662511 | 3.4:-99226423 | MS.gene68228:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACCTTGTGATTTCAATTTA+GGG | + | chr3.4:99226615-99226634 | None:intergenic | 25.0% |
| ! | CACTATAGTAGAAATTATGA+TGG | - | chr3.4:99226327-99226346 | MS.gene68228:CDS | 25.0% |
| ! | GAATTTACACAAAACATTCT+TGG | - | chr3.4:99226235-99226254 | MS.gene68228:CDS | 25.0% |
| ! | TAAAAAACAGCATAAGGATA+AGG | + | chr3.4:99226391-99226410 | None:intergenic | 25.0% |
| ! | TGACAATAAAAAACAGCATA+AGG | + | chr3.4:99226397-99226416 | None:intergenic | 25.0% |
| ! | TTGAAAAGTAACACTTGATT+TGG | + | chr3.4:99226302-99226321 | None:intergenic | 25.0% |
| !!! | AGGGTGTATAAAGTTTTATT+GGG | - | chr3.4:99226436-99226455 | MS.gene68228:CDS | 25.0% |
| !!! | TAGGGTGTATAAAGTTTTAT+TGG | - | chr3.4:99226435-99226454 | MS.gene68228:CDS | 25.0% |
| ACTTGAAATGAAACATGACT+AGG | + | chr3.4:99226526-99226545 | None:intergenic | 30.0% | |
| ATAGTCAAGAAAGTGAGAAT+AGG | - | chr3.4:99226416-99226435 | MS.gene68228:CDS | 30.0% | |
| GAACCTTGTGATTTCAATTT+AGG | + | chr3.4:99226616-99226635 | None:intergenic | 30.0% | |
| TAGTCAAGAAAGTGAGAATA+GGG | - | chr3.4:99226417-99226436 | MS.gene68228:CDS | 30.0% | |
| ! | TTCAAGCATAGATTCTAAAG+AGG | + | chr3.4:99226218-99226237 | None:intergenic | 30.0% |
| !! | CATGTTTCATTTCAAGTTCT+TGG | - | chr3.4:99226529-99226548 | MS.gene68228:CDS | 30.0% |
| !!! | GGGTGTATAAAGTTTTATTG+GGG | - | chr3.4:99226437-99226456 | MS.gene68228:CDS | 30.0% |
| !!! | GGTGTATAAAGTTTTATTGG+GGG | - | chr3.4:99226438-99226457 | MS.gene68228:CDS | 30.0% |
| !!! | GTGTATAAAGTTTTATTGGG+GGG | - | chr3.4:99226439-99226458 | MS.gene68228:CDS | 30.0% |
| AACTTAACCTCTCCTTTGAT+GGG | + | chr3.4:99226187-99226206 | None:intergenic | 35.0% | |
| GTCCCTAAATTGAAATCACA+AGG | - | chr3.4:99226610-99226629 | MS.gene68228:CDS | 35.0% | |
| TCACTTGGATACATCACAAT+GGG | - | chr3.4:99226498-99226517 | MS.gene68228:CDS | 35.0% | |
| !! | GCTGCTTTCACATTCTTTTA+AGG | + | chr3.4:99226161-99226180 | None:intergenic | 35.0% |
| GAACTTAACCTCTCCTTTGA+TGG | + | chr3.4:99226188-99226207 | None:intergenic | 40.0% | |
| GTCACTTGGATACATCACAA+TGG | - | chr3.4:99226497-99226516 | MS.gene68228:CDS | 40.0% | |
| TGAGAATGGAGATAAGGTTG+AGG | - | chr3.4:99226462-99226481 | MS.gene68228:CDS | 40.0% | |
| TGTGCAAAGTTGCATAAGCT+TGG | + | chr3.4:99226276-99226295 | None:intergenic | 40.0% | |
| !! | AATTCTAGCTCCAAAGATGG+TGG | - | chr3.4:99226351-99226370 | MS.gene68228:CDS | 40.0% |
| !! | GGAAATTCTAGCTCCAAAGA+TGG | - | chr3.4:99226348-99226367 | MS.gene68228:CDS | 40.0% |
| !!! | TGTGTGTCATTTTTTCCCAG+CGG | - | chr3.4:99226573-99226592 | MS.gene68228:CDS | 40.0% |
| ACCCAAATGTAATTGTCCGC+TGG | + | chr3.4:99226592-99226611 | None:intergenic | 45.0% | |
| AGATAAGGTTGAGGCTATGG+TGG | - | chr3.4:99226471-99226490 | MS.gene68228:CDS | 45.0% | |
| CCCAAATGTAATTGTCCGCT+GGG | + | chr3.4:99226591-99226610 | None:intergenic | 45.0% | |
| CCCAGCGGACAATTACATTT+GGG | - | chr3.4:99226588-99226607 | MS.gene68228:CDS | 45.0% | |
| GAAAGCAGCAAACCCATCAA+AGG | - | chr3.4:99226172-99226191 | MS.gene68228:CDS | 45.0% | |
| TCCCAGCGGACAATTACATT+TGG | - | chr3.4:99226587-99226606 | MS.gene68228:CDS | 45.0% | |
| TGGAGATAAGGTTGAGGCTA+TGG | - | chr3.4:99226468-99226487 | MS.gene68228:CDS | 45.0% | |
| !! | GTTTTATTGGGGGGTGAGAA+TGG | - | chr3.4:99226448-99226467 | MS.gene68228:CDS | 45.0% |
| CAGCAAACCCATCAAAGGAG+AGG | - | chr3.4:99226177-99226196 | MS.gene68228:CDS | 50.0% | |
| GTGTGACAAGCCACCATCTT+TGG | + | chr3.4:99226364-99226383 | None:intergenic | 50.0% | |
| TGACACACAGAAGAGCTTCC+TGG | + | chr3.4:99226562-99226581 | None:intergenic | 50.0% | |
| ! | GGCTATGGTGGTTTGTCACT+TGG | - | chr3.4:99226483-99226502 | MS.gene68228:CDS | 50.0% |
| !! | CAAGTTCTTGGTGTCACACC+AGG | - | chr3.4:99226541-99226560 | MS.gene68228:CDS | 50.0% |
| GGGGGGTGAGAATGGAGATA+AGG | - | chr3.4:99226456-99226475 | MS.gene68228:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 99226148 | 99226648 | 99226148 | ID=MS.gene68228 |
| chr3.4 | mRNA | 99226148 | 99226648 | 99226148 | ID=MS.gene68228.t1;Parent=MS.gene68228 |
| chr3.4 | exon | 99226148 | 99226648 | 99226148 | ID=MS.gene68228.t1.exon1;Parent=MS.gene68228.t1 |
| chr3.4 | CDS | 99226148 | 99226648 | 99226148 | ID=cds.MS.gene68228.t1;Parent=MS.gene68228.t1 |
| Gene Sequence |
| Protein sequence |
