Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68797.t1 | XP_024635707.1 | 85.7 | 265 | 36 | 2 | 1 | 265 | 1 | 263 | 4.10E-114 | 421 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68797.t1 | G7J762 | 85.7 | 265 | 36 | 2 | 1 | 265 | 1 | 263 | 2.9e-114 | 421.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene68797 | 0.800225 | 1.59E-48 | -1.69E-46 |
| MS.gene049887 | MS.gene68797 | 0.816309 | 5.91E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene68797 | 0.802503 | 5.44E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene68797 | 0.816729 | 4.76E-52 | -1.69E-46 |
| MS.gene052434 | MS.gene68797 | 0.80061 | 1.33E-48 | -1.69E-46 |
| MS.gene053677 | MS.gene68797 | 0.801313 | 9.55E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68797.t1 | MTR_3g117050 | 89.057 | 265 | 27 | 2 | 1 | 265 | 1 | 263 | 1.07e-166 | 462 |
| MS.gene68797.t1 | MTR_4g070660 | 41.445 | 263 | 98 | 13 | 3 | 255 | 5 | 221 | 3.44e-32 | 118 |
| MS.gene68797.t1 | MTR_4g070640 | 38.340 | 253 | 97 | 10 | 10 | 255 | 9 | 209 | 2.65e-25 | 100 |
| MS.gene68797.t1 | MTR_4g070630 | 36.967 | 211 | 77 | 9 | 49 | 252 | 2 | 163 | 8.59e-24 | 94.7 |
| MS.gene68797.t1 | MTR_8g077085 | 30.085 | 236 | 141 | 9 | 22 | 256 | 12 | 224 | 7.08e-20 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68797.t1 | AT5G66440 | 25.899 | 278 | 146 | 8 | 1 | 257 | 1 | 239 | 7.39e-20 | 86.3 |
| MS.gene68797.t1 | AT4G34560 | 31.527 | 203 | 92 | 8 | 64 | 264 | 63 | 220 | 2.87e-15 | 73.2 |
Find 34 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAGATTAGCTAGGAATAAA+TGG | 0.252040 | 3.2:-92457284 | None:intergenic |
| TCGTTGCTCATTCCATCTTC+AGG | 0.330037 | 3.2:-92457193 | None:intergenic |
| CGAGGTTTCGCGGTTGGTCC+TGG | 0.365762 | 3.2:+92456680 | MS.gene68797:CDS |
| GAAAGCTTGCAAATTTGTTT+CGG | 0.373892 | 3.2:+92456567 | MS.gene68797:CDS |
| GTGATAACGATAAGAGTAAG+AGG | 0.395005 | 3.2:+92457125 | MS.gene68797:CDS |
| CTTTGGAAGTGAAGGGATAT+AGG | 0.401542 | 3.2:+92457020 | MS.gene68797:CDS |
| ATCACACTCATTGCTCAATC+TGG | 0.416349 | 3.2:+92456734 | MS.gene68797:CDS |
| AACATGTATCAGAAAATTCA+AGG | 0.427396 | 3.2:+92456836 | MS.gene68797:CDS |
| AAAGTTGAGAACAACGTAAA+GGG | 0.443322 | 3.2:+92456884 | MS.gene68797:CDS |
| TCAAAGCCAACGGTAGTTGT+TGG | 0.443920 | 3.2:-92456637 | None:intergenic |
| AGATAATAACATAATGAAGT+TGG | 0.453775 | 3.2:+92456967 | MS.gene68797:CDS |
| AGTTTATTATATCCTGAAGA+TGG | 0.470634 | 3.2:+92457181 | MS.gene68797:CDS |
| GATGAAAACAGAGATGGCTT+TGG | 0.493344 | 3.2:+92457003 | MS.gene68797:CDS |
| TAGAATAAACGATGGATGCA+TGG | 0.507412 | 3.2:+92456910 | MS.gene68797:CDS |
| TTCATTCCAACAACTACCGT+TGG | 0.531596 | 3.2:+92456631 | MS.gene68797:CDS |
| AGAGATGGCTTTGGAAGTGA+AGG | 0.535480 | 3.2:+92457012 | MS.gene68797:CDS |
| GGGATATAGGAGGTGTCAAT+CGG | 0.539842 | 3.2:+92457033 | MS.gene68797:CDS |
| AAAAGTTGAGAACAACGTAA+AGG | 0.543362 | 3.2:+92456883 | MS.gene68797:CDS |
| CTCGAGTTCTTCAAAGCCAA+CGG | 0.560967 | 3.2:-92456647 | None:intergenic |
| ATCGGAAATTGAGTTGGTGA+GGG | 0.576160 | 3.2:+92457051 | MS.gene68797:CDS |
| TGAAGTTGGAAGAGAAACAA+GGG | 0.593425 | 3.2:+92456981 | MS.gene68797:CDS |
| AGGATCAAAGAGTGCTGCAA+AGG | 0.598659 | 3.2:+92457095 | MS.gene68797:CDS |
| AATCGGAAATTGAGTTGGTG+AGG | 0.606720 | 3.2:+92457050 | MS.gene68797:CDS |
| ATGAAGTTGGAAGAGAAACA+AGG | 0.609665 | 3.2:+92456980 | MS.gene68797:CDS |
| TGGAAGTGAAGGGATATAGG+AGG | 0.613637 | 3.2:+92457023 | MS.gene68797:CDS |
| ACAAGGGATGAAAACAGAGA+TGG | 0.618411 | 3.2:+92456997 | MS.gene68797:CDS |
| GTGTCAATCGGAAATTGAGT+TGG | 0.619603 | 3.2:+92457045 | MS.gene68797:CDS |
| TCTTGCAATAAATGCCTCCA+CGG | 0.627372 | 3.2:-92457230 | None:intergenic |
| TGTGAATAGTGATGATGTGA+AGG | 0.638060 | 3.2:+92457075 | MS.gene68797:CDS |
| TCGGAAATTGAGTTGGTGAG+GGG | 0.662410 | 3.2:+92457052 | MS.gene68797:CDS |
| CGAATTTCGTCGCACCGTGG+AGG | 0.663215 | 3.2:+92457216 | MS.gene68797:CDS |
| GAGATGGCTTTGGAAGTGAA+GGG | 0.676084 | 3.2:+92457013 | MS.gene68797:CDS |
| AAGGGTCGTAGAATAAACGA+TGG | 0.676337 | 3.2:+92456902 | MS.gene68797:CDS |
| CGACGAATTTCGTCGCACCG+TGG | 0.721139 | 3.2:+92457213 | MS.gene68797:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATAATAACATAATGAAGT+TGG | + | chr3.2:92456967-92456986 | MS.gene68797:CDS | 20.0% |
| ! | AACATGTATCAGAAAATTCA+AGG | + | chr3.2:92456836-92456855 | MS.gene68797:CDS | 25.0% |
| ! | AGTTTATTATATCCTGAAGA+TGG | + | chr3.2:92457181-92457200 | MS.gene68797:CDS | 25.0% |
| !!! | TTGTGTTGATTTTGATTTCA+AGG | + | chr3.2:92456606-92456625 | MS.gene68797:CDS | 25.0% |
| AAAAGTTGAGAACAACGTAA+AGG | + | chr3.2:92456883-92456902 | MS.gene68797:CDS | 30.0% | |
| AAAGTTGAGAACAACGTAAA+GGG | + | chr3.2:92456884-92456903 | MS.gene68797:CDS | 30.0% | |
| ! | GAAAGCTTGCAAATTTGTTT+CGG | + | chr3.2:92456567-92456586 | MS.gene68797:CDS | 30.0% |
| !! | ACGATGTTTTTGAACTTTGT+TGG | - | chr3.2:92456540-92456559 | None:intergenic | 30.0% |
| ATGAAGTTGGAAGAGAAACA+AGG | + | chr3.2:92456980-92456999 | MS.gene68797:CDS | 35.0% | |
| CCAACAAGAAAAACAAAACC+AGG | - | chr3.2:92456701-92456720 | None:intergenic | 35.0% | |
| TAGAATAAACGATGGATGCA+TGG | + | chr3.2:92456910-92456929 | MS.gene68797:CDS | 35.0% | |
| TGAAGTTGGAAGAGAAACAA+GGG | + | chr3.2:92456981-92457000 | MS.gene68797:CDS | 35.0% | |
| ! | AACTCGAGTGAATATTTTCG+AGG | + | chr3.2:92456662-92456681 | MS.gene68797:CDS | 35.0% |
| ! | GTGATAACGATAAGAGTAAG+AGG | + | chr3.2:92457125-92457144 | MS.gene68797:CDS | 35.0% |
| ! | TGTGAATAGTGATGATGTGA+AGG | + | chr3.2:92457075-92457094 | MS.gene68797:CDS | 35.0% |
| !! | TGAATATTTTCGAGGTTTCG+CGG | + | chr3.2:92456670-92456689 | MS.gene68797:CDS | 35.0% |
| !!! | CCTGGTTTTGTTTTTCTTGT+TGG | + | chr3.2:92456698-92456717 | MS.gene68797:CDS | 35.0% |
| AAGGGTCGTAGAATAAACGA+TGG | + | chr3.2:92456902-92456921 | MS.gene68797:CDS | 40.0% | |
| ACAAGGGATGAAAACAGAGA+TGG | + | chr3.2:92456997-92457016 | MS.gene68797:CDS | 40.0% | |
| ATCACACTCATTGCTCAATC+TGG | + | chr3.2:92456734-92456753 | MS.gene68797:CDS | 40.0% | |
| GATGAAAACAGAGATGGCTT+TGG | + | chr3.2:92457003-92457022 | MS.gene68797:CDS | 40.0% | |
| GTGTCAATCGGAAATTGAGT+TGG | + | chr3.2:92457045-92457064 | MS.gene68797:CDS | 40.0% | |
| TCTTGCAATAAATGCCTCCA+CGG | - | chr3.2:92457233-92457252 | None:intergenic | 40.0% | |
| TTCATTCCAACAACTACCGT+TGG | + | chr3.2:92456631-92456650 | MS.gene68797:CDS | 40.0% | |
| ! | CTTTGGAAGTGAAGGGATAT+AGG | + | chr3.2:92457020-92457039 | MS.gene68797:CDS | 40.0% |
| !! | AATCGGAAATTGAGTTGGTG+AGG | + | chr3.2:92457050-92457069 | MS.gene68797:CDS | 40.0% |
| !! | ATCGGAAATTGAGTTGGTGA+GGG | + | chr3.2:92457051-92457070 | MS.gene68797:CDS | 40.0% |
| CTCGAGTTCTTCAAAGCCAA+CGG | - | chr3.2:92456650-92456669 | None:intergenic | 45.0% | |
| TCAAAGCCAACGGTAGTTGT+TGG | - | chr3.2:92456640-92456659 | None:intergenic | 45.0% | |
| TCGTTGCTCATTCCATCTTC+AGG | - | chr3.2:92457196-92457215 | None:intergenic | 45.0% | |
| TGGAAGTGAAGGGATATAGG+AGG | + | chr3.2:92457023-92457042 | MS.gene68797:CDS | 45.0% | |
| !! | AGAGATGGCTTTGGAAGTGA+AGG | + | chr3.2:92457012-92457031 | MS.gene68797:CDS | 45.0% |
| !! | AGGATCAAAGAGTGCTGCAA+AGG | + | chr3.2:92457095-92457114 | MS.gene68797:CDS | 45.0% |
| !! | GAGATGGCTTTGGAAGTGAA+GGG | + | chr3.2:92457013-92457032 | MS.gene68797:CDS | 45.0% |
| !! | GGGATATAGGAGGTGTCAAT+CGG | + | chr3.2:92457033-92457052 | MS.gene68797:CDS | 45.0% |
| !! | TATTTTCGAGGTTTCGCGGT+TGG | + | chr3.2:92456674-92456693 | MS.gene68797:CDS | 45.0% |
| !! | TCGGAAATTGAGTTGGTGAG+GGG | + | chr3.2:92457052-92457071 | MS.gene68797:CDS | 45.0% |
| CGAATTTCGTCGCACCGTGG+AGG | + | chr3.2:92457216-92457235 | MS.gene68797:CDS | 60.0% | |
| CGACGAATTTCGTCGCACCG+TGG | + | chr3.2:92457213-92457232 | MS.gene68797:CDS | 60.0% | |
| ! | CGAGGTTTCGCGGTTGGTCC+TGG | + | chr3.2:92456680-92456699 | MS.gene68797:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 92456497 | 92457297 | 92456497 | ID=MS.gene68797 |
| chr3.2 | mRNA | 92456497 | 92457297 | 92456497 | ID=MS.gene68797.t1;Parent=MS.gene68797 |
| chr3.2 | exon | 92456497 | 92457297 | 92456497 | ID=MS.gene68797.t1.exon1;Parent=MS.gene68797.t1 |
| chr3.2 | CDS | 92456497 | 92457297 | 92456497 | ID=cds.MS.gene68797.t1;Parent=MS.gene68797.t1 |
| Gene Sequence |
| Protein sequence |
