AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051852


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051852.t1 MTR_4g125990 89.922 129 4 4 1 126 1 123 9.58e-75 218
MS.gene051852.t1 MTR_8g018320 50.000 120 42 3 3 122 2 103 1.77e-27 97.8
MS.gene051852.t1 MTR_2g099630 48.387 124 44 6 3 125 2 106 1.44e-26 95.5
MS.gene051852.t1 MTR_3g104640 52.756 127 42 4 1 126 1 110 1.78e-26 95.5
MS.gene051852.t1 MTR_8g018360 49.587 121 43 3 3 123 2 104 2.16e-26 97.4
MS.gene051852.t1 MTR_1g015810 50.000 128 48 5 1 124 1 116 1.45e-20 80.9
MS.gene051852.t1 MTR_7g109290 54.386 57 25 1 70 126 36 91 1.52e-17 72.0
MS.gene051852.t1 MTR_1g087730 51.923 52 25 0 75 126 55 106 2.68e-16 69.3
MS.gene051852.t1 MTR_1g098310 54.386 57 23 1 72 125 29 85 8.32e-12 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051852.t1 AT2G19460 53.846 130 40 6 1 124 1 116 3.82e-34 115
MS.gene051852.t1 AT2G19460 53.077 130 41 6 1 124 53 168 7.74e-34 115
MS.gene051852.t1 AT5G11970 51.181 127 38 6 1 126 1 104 2.27e-30 105
MS.gene051852.t1 AT3G13910 60.294 68 22 2 55 117 30 97 3.25e-19 76.6
MS.gene051852.t1 AT3G13910 60.294 68 22 2 55 117 30 97 6.16e-19 76.6
MS.gene051852.t1 AT3G05725 34.848 132 70 4 1 125 1 123 6.28e-17 71.6
MS.gene051852.t1 AT3G62640 48.077 52 27 0 74 125 59 110 7.25e-15 65.9
MS.gene051852.t1 AT1G72720 36.441 118 59 4 9 125 23 125 8.55e-15 66.2
MS.gene051852.t1 AT2G47480 45.902 61 33 0 64 124 48 108 9.91e-15 65.5
MS.gene051852.t1 AT4G09890 59.459 37 15 0 82 118 39 75 8.81e-11 54.7

Find 22 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCCATCAGCGTAGGATT+TGG 0.309332 8.2:+6567102 None:intergenic
TGTTGTTACCAATCTGGTTT+TGG 0.327376 8.2:+6566969 None:intergenic
TGTTGTTGTTGTTACCAATC+TGG 0.354115 8.2:+6566963 None:intergenic
GTAAACTCAAAGGCTCTCTT+AGG 0.367994 8.2:-6566814 MS.gene051852:CDS
AAGAAAGGTAAATCCACAAA+TGG 0.395506 8.2:-6566923 MS.gene051852:CDS
TACAAGGTTTATTCTGTTGA+AGG 0.413127 8.2:-6566836 MS.gene051852:CDS
GAAGAAGGTGCAGCAGAGTA+AGG 0.442974 8.2:+6567064 None:intergenic
AGATGCAACAGAGTTGTTCA+TGG 0.485910 8.2:-6566767 MS.gene051852:CDS
AACGATGTTAAGTTCAAGAA+AGG 0.501790 8.2:-6566938 MS.gene051852:CDS
ATGCCTCCTCCGTTTGAAGA+AGG 0.518627 8.2:+6567049 None:intergenic
AGAAAGGTAAATCCACAAAT+GGG 0.520575 8.2:-6566922 MS.gene051852:CDS
TCTGCTGCACCTTCTTCAAA+CGG 0.523824 8.2:-6567058 MS.gene051852:CDS
ACCCAAAACCAAAACCAGAT+TGG 0.525292 8.2:-6566977 MS.gene051852:CDS
CTGCATCCTTCCATCAGCGT+AGG 0.527015 8.2:+6567096 None:intergenic
GCAACAGAGTTGTTCATGGC+TGG 0.536888 8.2:-6566763 MS.gene051852:CDS
TTTGGATCTGAAATCTTCCA+TGG 0.557041 8.2:+6567120 None:intergenic
AGATCCAAATCCTACGCTGA+TGG 0.558533 8.2:-6567106 MS.gene051852:CDS
TCTGTTGAAGGTAAACTCAA+AGG 0.562516 8.2:-6566824 MS.gene051852:CDS
GCACCTTCTTCAAACGGAGG+AGG 0.581419 8.2:-6567052 MS.gene051852:CDS
CCAAATCCTACGCTGATGGA+AGG 0.584749 8.2:-6567102 MS.gene051852:CDS
TCAGTGATCCTGAATTGCAG+AGG 0.668157 8.2:-6566877 MS.gene051852:CDS
GCTGCACCTTCTTCAAACGG+AGG 0.686822 8.2:-6567055 MS.gene051852:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAAAGAGTTGCTAGTTACA+AGG - chr8.2:6567026-6567045 MS.gene051852:CDS 30.0%
AACGATGTTAAGTTCAAGAA+AGG - chr8.2:6566940-6566959 MS.gene051852:CDS 30.0%
AAGAAAGGTAAATCCACAAA+TGG - chr8.2:6566955-6566974 MS.gene051852:CDS 30.0%
AGAAAGGTAAATCCACAAAT+GGG - chr8.2:6566956-6566975 MS.gene051852:CDS 30.0%
TACAAGGTTTATTCTGTTGA+AGG - chr8.2:6567042-6567061 MS.gene051852:CDS 30.0%
ATGGGTCAACTTCAAAAAGT+TGG - chr8.2:6566974-6566993 MS.gene051852:CDS 35.0%
TCTGTTGAAGGTAAACTCAA+AGG - chr8.2:6567054-6567073 MS.gene051852:CDS 35.0%
! TGTTGTTGTTGTTACCAATC+TGG + chr8.2:6566918-6566937 None:intergenic 35.0%
!! TGTTGTTACCAATCTGGTTT+TGG + chr8.2:6566912-6566931 None:intergenic 35.0%
!!! ACCAATCTGGTTTTGGTTTT+GGG + chr8.2:6566905-6566924 None:intergenic 35.0%
!!! CTCTTAGGAAGAGTTTTAAG+TGG - chr8.2:6567079-6567098 MS.gene051852:CDS 35.0%
!!! CTTTTTTTCCTCTGCAATTC+AGG + chr8.2:6567012-6567031 None:intergenic 35.0%
!!! TACCAATCTGGTTTTGGTTT+TGG + chr8.2:6566906-6566925 None:intergenic 35.0%
!!! TTTTGAAGTTGACCCATTTG+TGG + chr8.2:6566971-6566990 None:intergenic 35.0%
ACCCAAAACCAAAACCAGAT+TGG - chr8.2:6566901-6566920 MS.gene051852:CDS 40.0%
AGATGCAACAGAGTTGTTCA+TGG - chr8.2:6567111-6567130 MS.gene051852:CDS 40.0%
GTAAACTCAAAGGCTCTCTT+AGG - chr8.2:6567064-6567083 MS.gene051852:CDS 40.0%
!!! ATCTGGTTTTGGTTTTGGGT+TGG + chr8.2:6566901-6566920 None:intergenic 40.0%
AGATCCAAATCCTACGCTGA+TGG - chr8.2:6566772-6566791 MS.gene051852:CDS 45.0%
TCAGTGATCCTGAATTGCAG+AGG - chr8.2:6567001-6567020 MS.gene051852:CDS 45.0%
TCTGCTGCACCTTCTTCAAA+CGG - chr8.2:6566820-6566839 MS.gene051852:CDS 45.0%
!!! GTTTTGGGTTGGATGAACAG+AGG + chr8.2:6566890-6566909 None:intergenic 45.0%
ATGCCTCCTCCGTTTGAAGA+AGG + chr8.2:6566832-6566851 None:intergenic 50.0%
CCAAATCCTACGCTGATGGA+AGG - chr8.2:6566776-6566795 MS.gene051852:CDS 50.0%
CCTTCCATCAGCGTAGGATT+TGG + chr8.2:6566779-6566798 None:intergenic 50.0%
GCAACAGAGTTGTTCATGGC+TGG - chr8.2:6567115-6567134 MS.gene051852:CDS 50.0%
! GAAGAAGGTGCAGCAGAGTA+AGG + chr8.2:6566817-6566836 None:intergenic 50.0%
CTGCATCCTTCCATCAGCGT+AGG + chr8.2:6566785-6566804 None:intergenic 55.0%
GCACCTTCTTCAAACGGAGG+AGG - chr8.2:6566826-6566845 MS.gene051852:CDS 55.0%
GCTGCACCTTCTTCAAACGG+AGG - chr8.2:6566823-6566842 MS.gene051852:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 6566760 6567140 6566760 ID=MS.gene051852
chr8.2 mRNA 6566760 6567140 6566760 ID=MS.gene051852.t1;Parent=MS.gene051852
chr8.2 exon 6566760 6567140 6566760 ID=MS.gene051852.t1.exon1;Parent=MS.gene051852.t1
chr8.2 CDS 6566760 6567140 6566760 ID=cds.MS.gene051852.t1;Parent=MS.gene051852.t1
Gene Sequence

>MS.gene051852

ATGGAAGATTTCAGATCCAAATCCTACGCTGATGGAAGGATGCAGCAAATTGAACCTTACTCTGCTGCACCTTCTTCAAACGGAGGAGGCATGCAAGATCTACGTTGTTACAGCGCTTCTTATGCTTCCTCTGTTCATCCAACCCAAAACCAAAACCAGATTGGTAACAACAACAACAACAACGATGTTAAGTTCAAGAAAGGTAAATCCACAAATGGGTCAACTTCAAAAAGTTGGAGCTTCAGTGATCCTGAATTGCAGAGGAAAAAAAGAGTTGCTAGTTACAAGGTTTATTCTGTTGAAGGTAAACTCAAAGGCTCTCTTAGGAAGAGTTTTAAGTGGATTAAAGATAGATGCAACAGAGTTGTTCATGGCTGGTAG

Protein sequence

>MS.gene051852.t1

MEDFRSKSYADGRMQQIEPYSAAPSSNGGGMQDLRCYSASYASSVHPTQNQNQIGNNNNNNDVKFKKGKSTNGSTSKSWSFSDPELQRKKRVASYKVYSVEGKLKGSLRKSFKWIKDRCNRVVHGW