AlfalfaGEDB | MS.gene68807

M. sativa cultivar XinJiangDaYe / MS.gene68807

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68807.t1	XP_013462329.1	88.4	259	21	1	4	262	137	386	1.20E-123	452.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68807.t1	Q9CAA9	52.0	196	70	6	61	255	297	469	1.7e-41	171.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68807.t1	A0A072V302	88.4	259	21	1	4	262	137	386	9.0e-124	452.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene68807.t1	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050076	MS.gene68807	0.81401	1.92E-51	-1.69E-46
MS.gene05101	MS.gene68807	0.836608	8.47E-57	-1.69E-46
MS.gene051471	MS.gene68807	0.802761	4.81E-49	-1.69E-46
MS.gene052326	MS.gene68807	0.809627	1.72E-50	-1.69E-46
MS.gene053836	MS.gene68807	0.812285	4.58E-51	-1.69E-46
MS.gene054473	MS.gene68807	0.806708	7.21E-50	-1.69E-46
MS.gene055428	MS.gene68807	0.823175	1.60E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68807.t1	MTR_3g116770	92.664	259	10	1	4	262	137	386	0.0	503
MS.gene68807.t1	MTR_3g116770	92.278	259	10	2	4	262	137	385	1.51e-178	497
MS.gene68807.t1	MTR_4g070320	65.660	265	63	11	4	257	82	329	2.73e-108	317
MS.gene68807.t1	MTR_5g048860	48.305	236	95	8	34	259	325	543	1.89e-56	189
MS.gene68807.t1	MTR_1g017350	45.249	221	89	4	27	247	220	408	4.65e-52	175
MS.gene68807.t1	MTR_1g017350	45.249	221	89	3	27	247	220	408	5.76e-52	175
MS.gene68807.t1	MTR_7g053410	45.560	259	114	7	7	259	270	507	9.26e-52	176
MS.gene68807.t1	MTR_8g065740	59.355	155	53	3	10	154	238	392	9.48e-52	175
MS.gene68807.t1	MTR_1g017350	45.701	221	85	3	27	247	220	405	1.03e-51	174
MS.gene68807.t1	MTR_1g017350	45.701	221	85	4	27	247	220	405	1.12e-51	174
MS.gene68807.t1	MTR_7g053410	45.560	259	114	7	7	259	270	507	1.24e-51	176
MS.gene68807.t1	MTR_8g012290	43.911	271	125	9	7	261	226	485	4.71e-49	169
MS.gene68807.t1	MTR_8g012290	43.173	271	126	9	7	261	226	484	3.04e-48	167
MS.gene68807.t1	MTR_5g037250	57.447	141	41	3	15	155	73	194	2.51e-43	147
MS.gene68807.t1	MTR_8g062240	58.904	146	48	3	29	168	108	247	2.97e-43	148
MS.gene68807.t1	MTR_3g498825	80.899	89	17	0	51	139	131	219	6.40e-43	149
MS.gene68807.t1	MTR_7g092510	61.017	118	38	1	43	160	157	266	1.37e-42	148
MS.gene68807.t1	MTR_8g099880	82.558	86	14	1	62	146	140	225	3.68e-42	147
MS.gene68807.t1	MTR_1g059270	67.010	97	32	0	60	156	153	249	1.51e-41	143
MS.gene68807.t1	MTR_1g059270	65.347	101	35	0	60	160	153	253	1.88e-41	145
MS.gene68807.t1	MTR_6g084120	60.227	88	24	2	63	148	151	229	6.95e-26	102
MS.gene68807.t1	MTR_4g131160	72.727	66	17	1	67	132	199	263	1.92e-23	98.6
MS.gene68807.t1	MTR_4g131160	72.727	66	17	1	67	132	196	260	1.96e-23	98.2
MS.gene68807.t1	MTR_1g080890	69.697	66	19	1	67	132	193	257	2.92e-22	94.7
MS.gene68807.t1	MTR_4g065870	41.071	112	60	2	26	131	308	419	6.28e-17	80.1
MS.gene68807.t1	MTR_2g098310	55.224	67	30	0	65	131	330	396	1.76e-16	78.6
MS.gene68807.t1	MTR_4g065870	40.367	109	59	2	26	128	308	416	3.08e-16	78.2
MS.gene68807.t1	MTR_2g098310	56.061	66	29	0	65	130	330	395	4.90e-16	77.4
MS.gene68807.t1	MTR_1g069155	52.174	69	32	1	69	137	319	386	7.05e-16	77.4
MS.gene68807.t1	MTR_4g033295	55.385	65	29	0	67	131	118	182	9.16e-16	73.9
MS.gene68807.t1	MTR_7g092540	34.228	149	87	5	1	140	170	316	1.49e-15	75.5
MS.gene68807.t1	MTR_1g084980	45.205	73	39	1	69	141	464	535	2.02e-15	76.3
MS.gene68807.t1	MTR_1g052470	53.623	69	32	0	67	135	298	366	2.12e-15	75.5
MS.gene68807.t1	MTR_4g033295	53.731	67	30	1	67	132	118	184	2.15e-15	72.8
MS.gene68807.t1	MTR_1g070870	65.152	66	22	1	67	132	120	184	5.36e-15	73.2
MS.gene68807.t1	MTR_1g070870	65.152	66	22	1	67	132	123	187	5.70e-15	73.2
MS.gene68807.t1	MTR_7g092540	34.532	139	80	5	1	130	170	306	7.76e-15	73.2
MS.gene68807.t1	MTR_7g099540	50.000	68	33	1	70	137	255	321	3.87e-14	72.0
MS.gene68807.t1	MTR_3g028520	46.753	77	41	0	55	131	310	386	6.96e-14	71.2
MS.gene68807.t1	MTR_3g027650	46.753	77	41	0	55	131	310	386	6.96e-14	71.2
MS.gene68807.t1	MTR_3g027650	47.297	74	39	0	55	128	310	383	3.77e-13	68.9
MS.gene68807.t1	MTR_8g036235	49.231	65	33	0	67	131	316	380	5.45e-13	68.6
MS.gene68807.t1	MTR_1g019240	47.692	65	33	1	69	133	87	150	6.26e-13	67.4
MS.gene68807.t1	MTR_4g107320	47.368	76	38	2	85	158	60	135	8.35e-13	64.7
MS.gene68807.t1	MTR_1g018090	44.828	87	45	2	45	131	254	337	1.40e-12	67.0
MS.gene68807.t1	MTR_3g449770	40.659	91	51	2	44	132	284	373	2.06e-12	67.0
MS.gene68807.t1	MTR_7g099540	49.206	63	31	1	70	132	255	316	3.21e-12	66.2
MS.gene68807.t1	MTR_7g111320	40.777	103	56	3	34	132	395	496	3.60e-12	66.2
MS.gene68807.t1	MTR_7g110810	36.111	108	59	3	78	185	213	310	1.20e-11	64.3
MS.gene68807.t1	MTR_1g116790	47.059	68	36	0	67	134	265	332	1.31e-11	64.3
MS.gene68807.t1	MTR_5g017040	53.571	56	25	1	78	133	116	170	1.87e-11	63.5
MS.gene68807.t1	MTR_5g005110	50.000	64	31	1	68	131	225	287	2.16e-11	63.5
MS.gene68807.t1	MTR_3g103030	51.562	64	30	1	68	131	230	292	2.22e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68807.t1	AT4G34530	47.390	249	87	8	7	242	113	330	8.68e-58	187
MS.gene68807.t1	AT1G68920	50.239	209	80	6	48	255	283	468	1.13e-54	183
MS.gene68807.t1	AT1G68920	50.239	209	80	6	48	255	284	469	1.14e-54	183
MS.gene68807.t1	AT1G68920	50.239	209	80	6	48	255	284	469	1.14e-54	183
MS.gene68807.t1	AT1G68920	50.239	209	80	6	48	255	284	469	1.14e-54	183
MS.gene68807.t1	AT3G07340	53.403	191	78	4	63	250	254	436	5.04e-53	178
MS.gene68807.t1	AT3G07340	52.632	190	81	3	63	249	254	437	2.38e-52	176
MS.gene68807.t1	AT2G18300	47.107	242	79	11	4	243	133	327	5.74e-52	172
MS.gene68807.t1	AT1G10120	47.465	217	74	6	30	246	170	346	1.29e-51	172
MS.gene68807.t1	AT1G10120	47.465	217	74	6	30	246	170	346	1.29e-51	172
MS.gene68807.t1	AT2G18300	61.333	150	45	5	4	152	133	270	2.68e-51	171
MS.gene68807.t1	AT2G18300	61.333	150	45	5	4	152	133	270	6.94e-51	171
MS.gene68807.t1	AT4G36540	56.494	154	46	3	3	156	98	230	4.12e-50	167
MS.gene68807.t1	AT4G36540	57.333	150	43	3	3	152	98	226	2.44e-49	165
MS.gene68807.t1	AT3G23690	60.417	144	50	4	13	154	140	278	7.67e-49	165
MS.gene68807.t1	AT5G48560	64.444	135	45	3	49	182	284	416	2.48e-48	166
MS.gene68807.t1	AT5G48560	68.595	121	36	2	63	182	297	416	5.36e-48	166
MS.gene68807.t1	AT1G26260	57.823	147	49	1	8	154	126	259	5.95e-48	162
MS.gene68807.t1	AT1G26260	57.823	147	49	1	8	154	155	288	1.24e-47	162
MS.gene68807.t1	AT1G26260	57.823	147	49	1	8	154	177	310	2.48e-47	162
MS.gene68807.t1	AT1G26260	57.823	147	49	1	8	154	177	310	2.48e-47	162
MS.gene68807.t1	AT1G26260	57.823	147	49	1	8	154	177	310	2.48e-47	162
MS.gene68807.t1	AT1G26260	57.823	147	49	1	8	154	177	310	2.48e-47	162
MS.gene68807.t1	AT5G50915	62.595	131	44	3	19	146	88	216	3.54e-46	155
MS.gene68807.t1	AT5G50915	62.595	131	44	3	19	146	88	216	3.54e-46	155
MS.gene68807.t1	AT5G50915	62.595	131	44	3	19	146	88	216	1.42e-45	154
MS.gene68807.t1	AT5G50915	62.595	131	44	3	19	146	88	216	1.42e-45	154
MS.gene68807.t1	AT1G59640	52.198	182	61	6	4	183	79	236	1.55e-44	151
MS.gene68807.t1	AT3G57800	50.888	169	75	2	33	194	170	337	2.68e-44	154
MS.gene68807.t1	AT1G59640	54.938	162	63	3	4	163	79	232	9.80e-44	151
MS.gene68807.t1	AT2G42300	70.968	93	27	0	61	153	179	271	2.72e-43	148
MS.gene68807.t1	AT2G42300	70.652	92	27	0	61	152	179	270	9.60e-42	145
MS.gene68807.t1	AT5G62610	81.579	76	14	0	63	138	149	224	1.37e-38	136
MS.gene68807.t1	AT5G62610	81.579	76	14	0	63	138	149	224	1.37e-38	136
MS.gene68807.t1	AT1G18400	75.000	80	20	0	65	144	143	222	3.49e-36	129
MS.gene68807.t1	AT1G73830	50.000	144	59	2	1	144	94	224	5.07e-36	129
MS.gene68807.t1	AT1G73830	50.000	144	59	2	1	144	94	224	5.84e-36	129
MS.gene68807.t1	AT1G18400	75.000	80	20	0	65	144	143	222	6.97e-36	129
MS.gene68807.t1	AT3G57800	39.815	216	75	3	33	194	170	384	2.61e-35	131
MS.gene68807.t1	AT1G25330	65.217	92	32	0	63	154	100	191	5.84e-35	125
MS.gene68807.t1	AT5G58010	65.000	80	23	2	67	142	99	177	2.20e-24	99.4
MS.gene68807.t1	AT2G24260	71.212	66	18	1	67	132	35	99	7.70e-24	97.1
MS.gene68807.t1	AT4G30980	72.727	66	17	1	67	132	130	194	1.38e-23	97.4
MS.gene68807.t1	AT4G30980	72.727	66	17	1	67	132	178	242	2.22e-23	97.8
MS.gene68807.t1	AT2G24260	71.212	66	18	1	67	132	138	202	4.48e-23	96.7
MS.gene68807.t1	AT2G42300	76.087	46	11	0	61	106	179	224	7.39e-19	83.2
MS.gene68807.t1	AT2G42300	76.087	46	11	0	61	106	179	224	7.39e-19	83.2
MS.gene68807.t1	AT2G42300	76.087	46	11	0	61	106	179	224	9.49e-19	82.8
MS.gene68807.t1	AT2G20180	35.714	112	71	1	21	132	161	271	1.05e-16	79.3
MS.gene68807.t1	AT2G20180	35.714	112	71	1	21	132	161	271	1.05e-16	79.3
MS.gene68807.t1	AT2G20180	35.714	112	71	1	21	132	161	271	1.05e-16	79.3
MS.gene68807.t1	AT2G42280	36.842	133	58	4	9	130	227	344	1.64e-16	78.6
MS.gene68807.t1	AT2G20180	35.714	112	71	1	21	132	232	342	1.73e-16	79.0
MS.gene68807.t1	AT2G20180	35.714	112	71	1	21	132	232	342	1.73e-16	79.0
MS.gene68807.t1	AT2G20180	35.714	112	71	1	21	132	232	342	1.73e-16	79.0
MS.gene68807.t1	AT2G20180	35.714	112	71	1	21	132	232	342	1.73e-16	79.0
MS.gene68807.t1	AT2G42280	35.878	131	60	3	9	130	227	342	1.90e-16	78.2
MS.gene68807.t1	AT1G51140	50.633	79	35	2	56	130	289	367	3.66e-15	74.7
MS.gene68807.t1	AT1G03040	50.549	91	44	1	42	132	114	203	5.37e-15	73.6
MS.gene68807.t1	AT2G20180	34.862	109	70	1	21	129	232	339	6.33e-15	73.9
MS.gene68807.t1	AT4G28800	33.803	142	69	3	70	211	252	368	8.37e-15	73.9
MS.gene68807.t1	AT1G09530	38.835	103	58	3	31	132	303	401	8.45e-15	73.9
MS.gene68807.t1	AT1G09530	38.835	103	58	3	31	132	303	401	8.45e-15	73.9
MS.gene68807.t1	AT1G09530	38.835	103	58	3	31	132	303	401	8.45e-15	73.9
MS.gene68807.t1	AT1G09530	38.835	103	58	3	31	132	303	401	8.45e-15	73.9
MS.gene68807.t1	AT1G09530	38.835	103	58	3	31	132	303	401	8.45e-15	73.9
MS.gene68807.t1	AT1G09530	38.835	103	58	3	31	132	303	401	8.45e-15	73.9
MS.gene68807.t1	AT4G09180	50.746	67	33	0	65	131	182	248	9.26e-15	72.4
MS.gene68807.t1	AT1G35460	52.308	65	31	0	67	131	181	245	1.17e-14	72.0
MS.gene68807.t1	AT4G28790	34.058	138	62	6	69	202	273	385	1.19e-14	73.6
MS.gene68807.t1	AT1G03040	60.274	73	28	1	60	132	137	208	1.32e-14	72.4
MS.gene68807.t1	AT4G02590	63.636	66	23	1	67	132	83	147	2.14e-14	71.2
MS.gene68807.t1	AT1G03040	60.274	73	28	1	60	132	137	208	2.35e-14	72.0
MS.gene68807.t1	AT4G28815	56.897	58	24	1	70	127	71	127	2.69e-14	70.9
MS.gene68807.t1	AT4G02590	63.636	66	23	1	67	132	146	210	3.07e-14	71.6
MS.gene68807.t1	AT4G02590	63.636	66	23	1	67	132	146	210	3.07e-14	71.6
MS.gene68807.t1	AT4G28815	56.897	58	24	1	70	127	147	203	7.97e-14	70.5
MS.gene68807.t1	AT4G28800	52.459	61	28	1	70	130	264	323	8.90e-13	68.2
MS.gene68807.t1	AT2G46970	29.016	193	92	8	57	226	187	357	9.20e-13	67.8
MS.gene68807.t1	AT2G46970	29.016	193	92	8	57	226	178	348	1.10e-12	67.4
MS.gene68807.t1	AT2G46970	29.016	193	92	8	57	226	209	379	1.21e-12	67.4
MS.gene68807.t1	AT4G33880	41.053	95	53	2	39	131	236	329	1.40e-12	67.0
MS.gene68807.t1	AT4G00050	50.769	65	31	1	67	131	105	168	3.80e-12	65.5
MS.gene68807.t1	AT2G43140	50.000	64	32	0	67	130	134	197	5.74e-12	63.9
MS.gene68807.t1	AT4G00050	50.769	65	31	1	67	131	207	270	6.41e-12	65.5
MS.gene68807.t1	AT4G28790	45.455	66	35	1	69	134	273	337	6.52e-12	65.1
MS.gene68807.t1	AT4G00050	50.769	65	31	1	67	131	207	270	6.60e-12	65.1
MS.gene68807.t1	AT2G43140	50.000	64	32	0	67	130	231	294	6.70e-12	64.7
MS.gene68807.t1	AT4G36930	50.820	61	29	1	73	133	197	256	8.96e-12	64.7
MS.gene68807.t1	AT2G14760	41.414	99	50	3	39	131	204	300	1.04e-11	64.3
MS.gene68807.t1	AT1G27740	34.711	121	72	3	11	131	117	230	1.29e-11	63.5
MS.gene68807.t1	AT1G05805	46.875	64	34	0	67	130	283	346	2.27e-11	63.5
MS.gene68807.t1	AT3G59060	34.188	117	70	3	70	185	253	363	3.40e-11	63.2
MS.gene68807.t1	AT3G59060	34.188	117	70	3	70	185	253	363	3.51e-11	63.2
MS.gene68807.t1	AT3G59060	34.188	117	70	3	70	185	253	363	3.51e-11	63.2
MS.gene68807.t1	AT3G59060	34.188	117	70	3	70	185	253	363	3.51e-11	63.2
MS.gene68807.t1	AT2G43010	39.583	96	50	2	42	130	219	313	5.82e-11	62.4
MS.gene68807.t1	AT2G43010	39.583	96	50	2	42	130	219	313	6.05e-11	62.4
MS.gene68807.t1	AT2G43010	39.583	96	50	2	42	130	226	320	6.53e-11	62.4
MS.gene68807.t1	AT2G43010	39.583	96	50	2	42	130	261	355	6.65e-11	62.4
MS.gene68807.t1	AT2G43010	39.583	96	50	2	42	130	219	313	7.10e-11	62.4

Find 47 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACCTTCCCATGTTGTATT+AGG	0.244216	3.2:-92354499	None:intergenic
CCACAGCAATTTGTTCCTTA+TGG	0.249421	3.2:+92354260	MS.gene68807:CDS
AATTGAAGTTGTATAGGTTT+TGG	0.253509	3.2:-92354525	None:intergenic
TCTCAATCTCAGTTATTTAC+AGG	0.262036	3.2:+92354577	MS.gene68807:CDS
AAAGTTTGAGGAATGGATAT+AGG	0.336253	3.2:-92354341	None:intergenic
GATCAAAATTGAAGTTGTAT+AGG	0.339897	3.2:-92354531	None:intergenic
ATTGGGATTCCCGGTGATCT+TGG	0.342453	3.2:-92353441	None:intergenic
AAGTTTGAGGAATGGATATA+GGG	0.345771	3.2:-92354340	None:intergenic
TGGAATGAACCCTACAGATA+TGG	0.359058	3.2:+92354295	MS.gene68807:CDS
CCAGGATGCAACAAAATTAC+AGG	0.386637	3.2:+92353770	MS.gene68807:CDS
AAAGACAAGAGGATCAAAAT+AGG	0.388939	3.2:+92353401	MS.gene68807:CDS
TATGTATGATGCAGGTTAGA+AGG	0.402202	3.2:+92353705	MS.gene68807:intron
TTGCTTGTTGTTTAGGCTGT+TGG	0.409201	3.2:+92353365	None:intergenic
CTTCTAAGTCCCATATCTGT+AGG	0.410368	3.2:-92354305	None:intergenic
AGCTATGGCTGTCGGTGGCT+TGG	0.431984	3.2:-92353595	None:intergenic
TGCTCGAACATGAATGTAAT+CGG	0.461397	3.2:-92353564	None:intergenic
GAAGAATCAATAAAAGTTTG+AGG	0.465715	3.2:-92354353	None:intergenic
ATCAATAAAAGTTTGAGGAA+TGG	0.483827	3.2:-92354348	None:intergenic
AACAAAATTACAGGAAAAGC+TGG	0.485342	3.2:+92353779	MS.gene68807:CDS
GGAATGAACCCTACAGATAT+GGG	0.494720	3.2:+92354296	MS.gene68807:CDS
ATGTATGATGCAGGTTAGAA+GGG	0.501649	3.2:+92353706	MS.gene68807:intron
CTACAGATATGGGACTTAGA+AGG	0.505239	3.2:+92354306	MS.gene68807:CDS
TTTGCATTTGCATCACAAGT+AGG	0.509857	3.2:-92354173	None:intergenic
TTCTAAGTCCCATATCTGTA+GGG	0.511108	3.2:-92354304	None:intergenic
TCAATATTGAAATCAAGCCT+TGG	0.519925	3.2:-92353999	None:intergenic
CCAGCATCTAATCCACCATA+AGG	0.520595	3.2:-92354275	None:intergenic
CAGCAATTTGTTCCTTATGG+TGG	0.522613	3.2:+92354263	MS.gene68807:CDS
TTACTCTTTCAGCTAAGCTA+TGG	0.530789	3.2:-92353610	None:intergenic
AGAAGATACTTCAAACTCAA+AGG	0.553682	3.2:+92353511	MS.gene68807:CDS
TTCAGCTAAGCTATGGCTGT+CGG	0.556309	3.2:-92353603	None:intergenic
CCTTATGGTGGATTAGATGC+TGG	0.561668	3.2:+92354275	MS.gene68807:CDS
TATCTGCAAGATTTAGTACC+AGG	0.562856	3.2:+92353752	MS.gene68807:CDS
GACGGCGAATCCAAGATCAC+CGG	0.574848	3.2:+92353431	MS.gene68807:CDS
GTAAATAACTGAGATTGAGA+AGG	0.584711	3.2:-92354574	None:intergenic
GGTGAATTGAACTGAAGATA+AGG	0.590017	3.2:-92354239	None:intergenic
CTACCTAATACAACATGGGA+AGG	0.593979	3.2:+92354496	MS.gene68807:CDS
AGCTAAGCTATGGCTGTCGG+TGG	0.605688	3.2:-92353600	None:intergenic
AATTAGCTACTGTTAATCCA+AGG	0.613412	3.2:+92353982	MS.gene68807:CDS
AAATTCTACCTAATACAACA+TGG	0.624191	3.2:+92354491	MS.gene68807:CDS
ACGGCGAATCCAAGATCACC+GGG	0.628691	3.2:+92353432	MS.gene68807:CDS
ATCAAAATAGGTGCTGAGGA+CGG	0.629527	3.2:+92353413	MS.gene68807:CDS
GAGGATCAAAATAGGTGCTG+AGG	0.635802	3.2:+92353409	MS.gene68807:CDS
AGTTTGAGGAATGGATATAG+GGG	0.638076	3.2:-92354339	None:intergenic
AAGTTGAGAACAAAGACAAG+AGG	0.638388	3.2:+92353390	MS.gene68807:CDS
ATTCATGTTCGAGCACGTCG+TGG	0.642577	3.2:+92353572	MS.gene68807:CDS
AATTCTACCTAATACAACAT+GGG	0.654432	3.2:+92354492	MS.gene68807:CDS
CCATAAGGAACAAATTGCTG+TGG	0.664587	3.2:-92354260	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAGTAGTAATAATATACTT+TGG	+	chr3.2:92353630-92353649	MS.gene68807:intron	15.0%
!!!	TAACATAAACAAATTTGTTA+TGG	-	chr3.2:92353936-92353955	None:intergenic	15.0%
!!	AAAATTAAAACACTAGTAAC+GGG	-	chr3.2:92354420-92354439	None:intergenic	20.0%
!!	ATTAAGCTAAAATACAGATT+AGG	-	chr3.2:92354083-92354102	None:intergenic	20.0%
!!	CAAAATTAAAACACTAGTAA+CGG	-	chr3.2:92354421-92354440	None:intergenic	20.0%
!!	TAATTATATATGTATGATGC+AGG	+	chr3.2:92353697-92353716	MS.gene68807:intron	20.0%
!!!	ATAAACAAATTTGTTATGGA+TGG	-	chr3.2:92353932-92353951	None:intergenic	20.0%
!!!	GTGTTTTAATTTTGTTGTAT+TGG	+	chr3.2:92354427-92354446	MS.gene68807:intron	20.0%
!	AAAAATATTAACAGAGAGAG+TGG	-	chr3.2:92354395-92354414	None:intergenic	25.0%
!	AAATTCTACCTAATACAACA+TGG	+	chr3.2:92354491-92354510	MS.gene68807:CDS	25.0%
!	AATTCTACCTAATACAACAT+GGG	+	chr3.2:92354492-92354511	MS.gene68807:CDS	25.0%
!	ATCAATAAAAGTTTGAGGAA+TGG	-	chr3.2:92354351-92354370	None:intergenic	25.0%
!	GAAGAATCAATAAAAGTTTG+AGG	-	chr3.2:92354356-92354375	None:intergenic	25.0%
!	GATCAAAATTGAAGTTGTAT+AGG	-	chr3.2:92354534-92354553	None:intergenic	25.0%
!	TGATGATCTATTTGAAAAAG+AGG	+	chr3.2:92354019-92354038	MS.gene68807:CDS	25.0%
!!	AATTGAAGTTGTATAGGTTT+TGG	-	chr3.2:92354528-92354547	None:intergenic	25.0%
!!!	AAATGCAAATTTTCAAGCTA+TGG	+	chr3.2:92354187-92354206	MS.gene68807:CDS	25.0%
!!!	AATGCAAATTTTCAAGCTAT+GGG	+	chr3.2:92354188-92354207	MS.gene68807:CDS	25.0%
!!!	TTGTTGTTCTTTTTGGTATT+GGG	-	chr3.2:92353461-92353480	None:intergenic	25.0%
	AAAGACAAGAGGATCAAAAT+AGG	+	chr3.2:92353401-92353420	MS.gene68807:CDS	30.0%
	AAAGTTTGAGGAATGGATAT+AGG	-	chr3.2:92354344-92354363	None:intergenic	30.0%
	AACAAAATTACAGGAAAAGC+TGG	+	chr3.2:92353779-92353798	MS.gene68807:CDS	30.0%
	AAGTTTGAGGAATGGATATA+GGG	-	chr3.2:92354343-92354362	None:intergenic	30.0%
	AATTAGCTACTGTTAATCCA+AGG	+	chr3.2:92353982-92354001	MS.gene68807:CDS	30.0%
	AGAAGATACTTCAAACTCAA+AGG	+	chr3.2:92353511-92353530	MS.gene68807:CDS	30.0%
	CATAGATAGATCAATGTGAT+AGG	-	chr3.2:92353907-92353926	None:intergenic	30.0%
	GGTAATAAAGAATATGCAGA+TGG	+	chr3.2:92354040-92354059	MS.gene68807:intron	30.0%
	GTAAATAACTGAGATTGAGA+AGG	-	chr3.2:92354577-92354596	None:intergenic	30.0%
	TCAATATTGAAATCAAGCCT+TGG	-	chr3.2:92354002-92354021	None:intergenic	30.0%
	TCTCAATCTCAGTTATTTAC+AGG	+	chr3.2:92354577-92354596	MS.gene68807:CDS	30.0%
!!	GTTGTTGTTCTTTTTGGTAT+TGG	-	chr3.2:92353462-92353481	None:intergenic	30.0%
!!	GTTGTTGTTGTTGTTCTTTT+TGG	-	chr3.2:92353468-92353487	None:intergenic	30.0%
!!	TTTGTGTTGATTCCTTACAT+AGG	+	chr3.2:92354145-92354164	MS.gene68807:intron	30.0%
	AAGTAGGAAACACCTATGTA+AGG	-	chr3.2:92354160-92354179	None:intergenic	35.0%
	AAGTTGAGAACAAAGACAAG+AGG	+	chr3.2:92353390-92353409	MS.gene68807:CDS	35.0%
	AGTTTGAGGAATGGATATAG+GGG	-	chr3.2:92354342-92354361	None:intergenic	35.0%
	ATGTATGATGCAGGTTAGAA+GGG	+	chr3.2:92353706-92353725	MS.gene68807:intron	35.0%
	GGTGAATTGAACTGAAGATA+AGG	-	chr3.2:92354242-92354261	None:intergenic	35.0%
	GTCTCTTCAAAAACAAGTTG+AGG	+	chr3.2:92353832-92353851	MS.gene68807:CDS	35.0%
	TATCTGCAAGATTTAGTACC+AGG	+	chr3.2:92353752-92353771	MS.gene68807:CDS	35.0%
	TATGTATGATGCAGGTTAGA+AGG	+	chr3.2:92353705-92353724	MS.gene68807:intron	35.0%
	TGCTCGAACATGAATGTAAT+CGG	-	chr3.2:92353567-92353586	None:intergenic	35.0%
	TTACTCTTTCAGCTAAGCTA+TGG	-	chr3.2:92353613-92353632	None:intergenic	35.0%
	TTCTAAGTCCCATATCTGTA+GGG	-	chr3.2:92354307-92354326	None:intergenic	35.0%
	TTTGCATTTGCATCACAAGT+AGG	-	chr3.2:92354176-92354195	None:intergenic	35.0%
!	GATTCTTCTTGTTTCTCTGT+AGG	+	chr3.2:92354368-92354387	MS.gene68807:intron	35.0%
	CCAGGATGCAACAAAATTAC+AGG	+	chr3.2:92353770-92353789	MS.gene68807:CDS	40.0%
	CCATAAGGAACAAATTGCTG+TGG	-	chr3.2:92354263-92354282	None:intergenic	40.0%
	CTACAGATATGGGACTTAGA+AGG	+	chr3.2:92354306-92354325	MS.gene68807:CDS	40.0%
	CTACCTAATACAACATGGGA+AGG	+	chr3.2:92354496-92354515	MS.gene68807:CDS	40.0%
	CTTCTAAGTCCCATATCTGT+AGG	-	chr3.2:92354308-92354327	None:intergenic	40.0%
	GGAATGAACCCTACAGATAT+GGG	+	chr3.2:92354296-92354315	MS.gene68807:CDS	40.0%
	TCACCTTCCCATGTTGTATT+AGG	-	chr3.2:92354502-92354521	None:intergenic	40.0%
	TGGAATGAACCCTACAGATA+TGG	+	chr3.2:92354295-92354314	MS.gene68807:CDS	40.0%
!	CAGCAATTTGTTCCTTATGG+TGG	+	chr3.2:92354263-92354282	MS.gene68807:CDS	40.0%
!	CCACAGCAATTTGTTCCTTA+TGG	+	chr3.2:92354260-92354279	MS.gene68807:CDS	40.0%
!	CCTGTAATTTTGTTGCATCC+TGG	-	chr3.2:92353773-92353792	None:intergenic	40.0%
!!	ATCAAAATAGGTGCTGAGGA+CGG	+	chr3.2:92353413-92353432	MS.gene68807:CDS	40.0%
!!	CTTTTTGGTATTGGGATTCC+CGG	-	chr3.2:92353453-92353472	None:intergenic	40.0%
	CCAGCATCTAATCCACCATA+AGG	-	chr3.2:92354278-92354297	None:intergenic	45.0%
	TTCAGCTAAGCTATGGCTGT+CGG	-	chr3.2:92353606-92353625	None:intergenic	45.0%
!	GAGGATCAAAATAGGTGCTG+AGG	+	chr3.2:92353409-92353428	MS.gene68807:CDS	45.0%
!!	CCTTATGGTGGATTAGATGC+TGG	+	chr3.2:92354275-92354294	MS.gene68807:CDS	45.0%
	ATTCATGTTCGAGCACGTCG+TGG	+	chr3.2:92353572-92353591	MS.gene68807:CDS	50.0%
	ATTGGGATTCCCGGTGATCT+TGG	-	chr3.2:92353444-92353463	None:intergenic	50.0%
	ACGGCGAATCCAAGATCACC+GGG	+	chr3.2:92353432-92353451	MS.gene68807:CDS	55.0%
	AGCTAAGCTATGGCTGTCGG+TGG	-	chr3.2:92353603-92353622	None:intergenic	55.0%
	GACGGCGAATCCAAGATCAC+CGG	+	chr3.2:92353431-92353450	MS.gene68807:CDS	55.0%
	AGCTATGGCTGTCGGTGGCT+TGG	-	chr3.2:92353598-92353617	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	92353380	92354598	92353380	ID=MS.gene68807
chr3.2	mRNA	92353380	92354598	92353380	ID=MS.gene68807.t1;Parent=MS.gene68807
chr3.2	exon	92353380	92353628	92353380	ID=MS.gene68807.t1.exon1;Parent=MS.gene68807.t1
chr3.2	CDS	92353380	92353628	92353380	ID=cds.MS.gene68807.t1;Parent=MS.gene68807.t1
chr3.2	exon	92353719	92353853	92353719	ID=MS.gene68807.t1.exon2;Parent=MS.gene68807.t1
chr3.2	CDS	92353719	92353853	92353719	ID=cds.MS.gene68807.t1;Parent=MS.gene68807.t1
chr3.2	exon	92353969	92354040	92353969	ID=MS.gene68807.t1.exon3;Parent=MS.gene68807.t1
chr3.2	CDS	92353969	92354040	92353969	ID=cds.MS.gene68807.t1;Parent=MS.gene68807.t1
chr3.2	exon	92354167	92354385	92354167	ID=MS.gene68807.t1.exon4;Parent=MS.gene68807.t1
chr3.2	CDS	92354167	92354385	92354167	ID=cds.MS.gene68807.t1;Parent=MS.gene68807.t1
chr3.2	exon	92354487	92354598	92354487	ID=MS.gene68807.t1.exon5;Parent=MS.gene68807.t1
chr3.2	CDS	92354487	92354598	92354487	ID=cds.MS.gene68807.t1;Parent=MS.gene68807.t1

Gene Sequence

>MS.gene68807

GCTGTTGGTGAAGTTGAGAACAAAGACAAGAGGATCAAAATAGGTGCTGAGGACGGCGAATCCAAGATCACCGGGAATCCCAATACCAAAAAGAACAACAACAACAACAACAACAAAAAAAACTGTGTAGCAGAAGATACTTCAAACTCAAAGGAAAATTCAAAAGTTTCTGATGTTCAAAAGACCGATTACATTCATGTTCGAGCACGTCGTGGCCAAGCCACCGACAGCCATAGCTTAGCTGAAAGAGTAAGTAGTAATAATATACTTTGGTTTCTGTCTGACTGAGAAAAGTAAAATCAAAGTATCTGAATAATTAATTATATATGTATGATGCAGGTTAGAAGGGAAAAGATTAGTGAGAGAATGAAGTATCTGCAAGATTTAGTACCAGGATGCAACAAAATTACAGGAAAAGCTGGAATGCTTGATGAAATCATCAACTATGTTCAGTCTCTTCAAAAACAAGTTGAGGTAATTAAAAGAGTTAATTAATTAAGTTCATTACATTGATTCTTAATTTGCCTATCACATTGATCTATCTATGATCCATCCATAACAAATTTGTTTATGTTAATTTTCTTTGCAGTTCTTGTCAATGAAATTAGCTACTGTTAATCCAAGGCTTGATTTCAATATTGATGATCTATTTGAAAAAGAGGTAATAAAGAATATGCAGATGGAAGAATTTCACTTTTCACCTAATCTGTATTTTAGCTTAATTTGAAATCTGTAATATAATATTAATATTAATATATTCTTCTATTTGTGTTGATTCCTTACATAGGTGTTTCCTACTTGTGATGCAAATGCAAATTTTCAAGCTATGGGAATGTCAACAGAATTGAATAGTAACAATCCTTATCTTCAGTTCAATTCACCACAGCAATTTGTTCCTTATGGTGGATTAGATGCTGGAATGAACCCTACAGATATGGGACTTAGAAGGAGCATAAGTGCCCCTATATCCATTCCTCAAACTTTTATTGATTCTTCTTGTTTCTCTGTAGGTCCACTCTCTCTGTTAATATTTTTGTCCCGTTACTAGTGTTTTAATTTTGTTGTATTGGTTTATTTTATTAATTTTATCTTTGTTTATGTGTGTAGCAGCAAATTCTACCTAATACAACATGGGAAGGTGATTTCCAAAACCTATACAACTTCAATTTTGATCAAGCAAGAGCAACATCTTTTCCTTCTCAATCTCAGTTATTTACAG

Protein sequence

>MS.gene68807.t1

AVGEVENKDKRIKIGAEDGESKITGNPNTKKNNNNNNNKKNCVAEDTSNSKENSKVSDVQKTDYIHVRARRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQKQVEFLSMKLATVNPRLDFNIDDLFEKEVFPTCDANANFQAMGMSTELNSNNPYLQFNSPQQFVPYGGLDAGMNPTDMGLRRSISAPISIPQTFIDSSCFSQQILPNTTWEGDFQNLYNFNFDQARATSFPSQSQLFT