Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69404.t1 | XP_003603750.2 | 96.8 | 217 | 4 | 1 | 1 | 217 | 1 | 214 | 7.50E-90 | 340.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69404.t1 | F4I0N3 | 36.7 | 218 | 95 | 8 | 8 | 210 | 1 | 190 | 1.3e-18 | 94.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69404.t1 | G7J4R0 | 96.8 | 217 | 4 | 1 | 1 | 217 | 1 | 214 | 5.4e-90 | 340.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055371 | MS.gene69404 | 0.812749 | 3.62E-51 | -1.69E-46 |
| MS.gene058909 | MS.gene69404 | 0.844218 | 8.66E-59 | -1.69E-46 |
| MS.gene058912 | MS.gene69404 | 0.842273 | 2.86E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69404.t1 | MTR_3g113810 | 96.774 | 217 | 4 | 1 | 1 | 217 | 1 | 214 | 1.46e-152 | 422 |
| MS.gene69404.t1 | MTR_5g021990 | 45.946 | 222 | 102 | 10 | 1 | 216 | 1 | 210 | 3.41e-46 | 152 |
| MS.gene69404.t1 | MTR_4g040170 | 96.970 | 66 | 2 | 0 | 152 | 217 | 1 | 66 | 2.23e-42 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69404.t1 | AT1G75720 | 43.519 | 216 | 83 | 8 | 8 | 210 | 1 | 190 | 2.78e-36 | 126 |
| MS.gene69404.t1 | AT1G75720 | 43.981 | 216 | 84 | 9 | 8 | 210 | 1 | 192 | 7.35e-36 | 125 |
| MS.gene69404.t1 | AT3G51220 | 35.096 | 208 | 100 | 7 | 15 | 212 | 4 | 186 | 4.09e-27 | 102 |
| MS.gene69404.t1 | AT2G17940 | 33.163 | 196 | 120 | 4 | 16 | 205 | 13 | 203 | 5.87e-23 | 92.4 |
| MS.gene69404.t1 | AT1G75720 | 43.791 | 153 | 57 | 6 | 71 | 210 | 12 | 148 | 1.70e-20 | 84.3 |
Find 38 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGTCTATCTCTGTATTTC+TGG | 0.104808 | 3.3:+93183839 | None:intergenic |
| CATAATGGAGCAAATGAAAA+TGG | 0.193439 | 3.3:-93182851 | MS.gene69404:CDS |
| TAAAGAAGAAAGCATGTTAA+TGG | 0.275611 | 3.3:-93182756 | MS.gene69404:CDS |
| TCATTCATCATGGAACTTCT+TGG | 0.313646 | 3.3:+93182372 | None:intergenic |
| TGGAAAGGCACCCTTCTCTT+AGG | 0.324014 | 3.3:-93182460 | MS.gene69404:CDS |
| AAAGAGGCTGTTACATTATT+CGG | 0.326753 | 3.3:-93183795 | MS.gene69404:CDS |
| TAGACACAAGGGCGCCATTC+AGG | 0.343210 | 3.3:-93183824 | MS.gene69404:CDS |
| TCGTGCGTTCGAGTTCTTCC+TGG | 0.369083 | 3.3:+93182714 | None:intergenic |
| AAGTCATTAATTCCACTTAT+CGG | 0.388786 | 3.3:-93182425 | MS.gene69404:CDS |
| ATAATGGAGCAAATGAAAAT+GGG | 0.406872 | 3.3:-93182850 | MS.gene69404:CDS |
| TTCTTCTTCTTCCTAAGAGA+AGG | 0.423924 | 3.3:+93182449 | None:intergenic |
| TGATGGAGTTTCAAAAGAAA+AGG | 0.446231 | 3.3:-93182556 | MS.gene69404:CDS |
| TCATCAAGGTGTTGAGAAGT+TGG | 0.449442 | 3.3:-93182480 | MS.gene69404:CDS |
| AGGTAACATGACATGAGAAA+TGG | 0.454194 | 3.3:+93182508 | None:intergenic |
| ATTTGATGAAGAATTAATGA+TGG | 0.459950 | 3.3:-93182573 | MS.gene69404:CDS |
| TCTAATCTATAGATTCATAA+TGG | 0.462589 | 3.3:-93182866 | MS.gene69404:intron |
| TCTTCTTCTTCCTAAGAGAA+GGG | 0.487269 | 3.3:+93182450 | None:intergenic |
| ATGGGGTTGATCAGTCTTCA+AGG | 0.494065 | 3.3:-93182832 | MS.gene69404:CDS |
| TAATGTAACAGCCTCTTTGA+CGG | 0.499377 | 3.3:+93183800 | None:intergenic |
| TGAAAACGTTACAGCTGAGC+TGG | 0.505652 | 3.3:-93182807 | MS.gene69404:CDS |
| ACATTGTATGTCATCTCTCC+AGG | 0.522311 | 3.3:-93182732 | MS.gene69404:CDS |
| AAAGATGAATAAGAAGAAAG+AGG | 0.535423 | 3.3:-93183877 | None:intergenic |
| GCCTCTTTGACGGACCTGAA+TGG | 0.535948 | 3.3:+93183810 | None:intergenic |
| AAGATGAATAAGAAGAAAGA+GGG | 0.553271 | 3.3:-93183876 | MS.gene69404:CDS |
| GCCATTCAGGTCCGTCAAAG+AGG | 0.580083 | 3.3:-93183811 | MS.gene69404:CDS |
| ATGTTACCTCAAGGTCATCA+AGG | 0.584789 | 3.3:-93182494 | MS.gene69404:CDS |
| TCTCATGTCATGTTACCTCA+AGG | 0.592396 | 3.3:-93182503 | MS.gene69404:CDS |
| TGGGGTTGATCAGTCTTCAA+GGG | 0.595004 | 3.3:-93182831 | MS.gene69404:CDS |
| TCAACACCTTGATGACCTTG+AGG | 0.616815 | 3.3:+93182488 | None:intergenic |
| AAGGTGTTGAGAAGTTGGAA+AGG | 0.636189 | 3.3:-93182475 | MS.gene69404:CDS |
| TAATGGAGCAAATGAAAATG+GGG | 0.636381 | 3.3:-93182849 | MS.gene69404:CDS |
| TCATTAATTCCACTTATCGG+AGG | 0.648652 | 3.3:-93182422 | MS.gene69404:CDS |
| TGCAACTAAACTCAAACACC+AGG | 0.653524 | 3.3:-93183739 | MS.gene69404:intron |
| AAACGTTACAGCTGAGCTGG+AGG | 0.666569 | 3.3:-93182804 | MS.gene69404:CDS |
| ATGACATGAGAAATGGACGG+TGG | 0.709324 | 3.3:+93182515 | None:intergenic |
| GAAATACAGAGATAGACACA+AGG | 0.731685 | 3.3:-93183836 | MS.gene69404:CDS |
| AAATACAGAGATAGACACAA+GGG | 0.731958 | 3.3:-93183835 | MS.gene69404:CDS |
| AACATGACATGAGAAATGGA+CGG | 0.745302 | 3.3:+93182512 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAATTATTAATAATGTG+TGG | + | chr3.3:93183347-93183366 | None:intergenic | 10.0% |
| !! | AAATTTAATTATTGATCCTT+AGG | - | chr3.3:93183149-93183168 | MS.gene69404:intron | 15.0% |
| !! | ATTATAATATGTTTGTAAGT+GGG | - | chr3.3:93182693-93182712 | MS.gene69404:CDS | 15.0% |
| !! | TATAAAATATAACCATCTAA+TGG | + | chr3.3:93182779-93182798 | None:intergenic | 15.0% |
| !!! | ATTTTATTAGAGTATCAAAA+TGG | - | chr3.3:93182560-93182579 | MS.gene69404:CDS | 15.0% |
| !! | AATAAAATTGAATGATGTGA+TGG | + | chr3.3:93182548-93182567 | None:intergenic | 20.0% |
| !! | ATTTGATGAAGAATTAATGA+TGG | - | chr3.3:93183679-93183698 | MS.gene69404:intron | 20.0% |
| !! | CATTATAATATGTTTGTAAG+TGG | - | chr3.3:93182692-93182711 | MS.gene69404:CDS | 20.0% |
| !! | TACTCAAATTCTTAATCATT+TGG | - | chr3.3:93183122-93183141 | MS.gene69404:intron | 20.0% |
| !! | TCTAATCTATAGATTCATAA+TGG | - | chr3.3:93183386-93183405 | MS.gene69404:intron | 20.0% |
| !! | TTAGAGAATAGAGATTAATA+AGG | - | chr3.3:93183263-93183282 | MS.gene69404:intron | 20.0% |
| !! | TTATAATATGTTTGTAAGTG+GGG | - | chr3.3:93182694-93182713 | MS.gene69404:CDS | 20.0% |
| !! | TTTAAATCACCAATTAAACT+TGG | - | chr3.3:93182604-93182623 | MS.gene69404:CDS | 20.0% |
| !!! | TAAGATGCACAAAATAATTT+TGG | + | chr3.3:93183246-93183265 | None:intergenic | 20.0% |
| !!! | TTCAATATAGTCTTACAATA+TGG | - | chr3.3:93183079-93183098 | MS.gene69404:intron | 20.0% |
| !!! | TTTTACTTTTTACATACCTA+AGG | + | chr3.3:93183168-93183187 | None:intergenic | 20.0% |
| ! | AAGTCATTAATTCCACTTAT+CGG | - | chr3.3:93183827-93183846 | MS.gene69404:CDS | 25.0% |
| ! | AATCTATAGATTAGAAGACA+TGG | + | chr3.3:93183381-93183400 | None:intergenic | 25.0% |
| ! | ACTTCCTTTATTCAATTAGA+CGG | + | chr3.3:93182984-93183003 | None:intergenic | 25.0% |
| ! | GTAACTCAATCTTACAAAAT+CGG | + | chr3.3:93182738-93182757 | None:intergenic | 25.0% |
| ! | GTTTCAAAATAACATAGGAT+AGG | + | chr3.3:93182876-93182895 | None:intergenic | 25.0% |
| ! | TCCAAATATTATCCATTAGA+TGG | - | chr3.3:93182764-93182783 | MS.gene69404:CDS | 25.0% |
| ! | TTAAAAAAATAATGGCCAAG+AGG | - | chr3.3:93182805-93182824 | MS.gene69404:CDS | 25.0% |
| ! | TTACTGTGTAAGATTAGTAT+CGG | - | chr3.3:93183196-93183215 | MS.gene69404:intron | 25.0% |
| ! | TTTACCGTCTAATTGAATAA+AGG | - | chr3.3:93182977-93182996 | MS.gene69404:intron | 25.0% |
| !! | ACCATCTAATGGATAATATT+TGG | + | chr3.3:93182768-93182787 | None:intergenic | 25.0% |
| !! | ATAATGGAGCAAATGAAAAT+GGG | - | chr3.3:93183402-93183421 | MS.gene69404:intron | 25.0% |
| !! | ATTAGAGTATCAAAATGGTT+AGG | - | chr3.3:93182565-93182584 | MS.gene69404:CDS | 25.0% |
| !! | TAAAGAAGAAAGCATGTTAA+TGG | - | chr3.3:93183496-93183515 | MS.gene69404:intron | 25.0% |
| !!! | AGGTATTTTTTCTAGGAAAA+AGG | - | chr3.3:93183850-93183869 | MS.gene69404:CDS | 25.0% |
| !!! | ATCCTATGTTATTTTGAAAC+AGG | - | chr3.3:93182876-93182895 | MS.gene69404:intron | 25.0% |
| !!! | GGTATTTTTTCTAGGAAAAA+GGG | - | chr3.3:93183851-93183870 | MS.gene69404:CDS | 25.0% |
| !!! | TCCTATGTTATTTTGAAACA+GGG | - | chr3.3:93182877-93182896 | MS.gene69404:intron | 25.0% |
| AAAGAGGCTGTTACATTATT+CGG | - | chr3.3:93182457-93182476 | MS.gene69404:CDS | 30.0% | |
| AAATACAGAGATAGACACAA+GGG | - | chr3.3:93182417-93182436 | MS.gene69404:CDS | 30.0% | |
| ACCCTGTTTCAAAATAACAT+AGG | + | chr3.3:93182881-93182900 | None:intergenic | 30.0% | |
| CGAAACTGAAAAAAATCAAG+TGG | - | chr3.3:93183589-93183608 | MS.gene69404:intron | 30.0% | |
| TAATGGAGCAAATGAAAATG+GGG | - | chr3.3:93183403-93183422 | MS.gene69404:intron | 30.0% | |
| TGATGGAGTTTCAAAAGAAA+AGG | - | chr3.3:93183696-93183715 | MS.gene69404:intron | 30.0% | |
| ! | CATAATGGAGCAAATGAAAA+TGG | - | chr3.3:93183401-93183420 | MS.gene69404:intron | 30.0% |
| ! | TCTTGAACTTTTCACTTCAA+AGG | + | chr3.3:93183660-93183679 | None:intergenic | 30.0% |
| !! | TCTTACAAAATCGGTTTGTA+AGG | + | chr3.3:93182729-93182748 | None:intergenic | 30.0% |
| !!! | TTATCGGAGGTATTTTTTCT+AGG | - | chr3.3:93183843-93183862 | MS.gene69404:CDS | 30.0% |
| AAATGTCCAAGAATTCCTCT+TGG | + | chr3.3:93182823-93182842 | None:intergenic | 35.0% | |
| AACATGACATGAGAAATGGA+CGG | + | chr3.3:93183743-93183762 | None:intergenic | 35.0% | |
| AGGAGAATCTTATTGATGGA+TGG | + | chr3.3:93182856-93182875 | None:intergenic | 35.0% | |
| AGGTAACATGACATGAGAAA+TGG | + | chr3.3:93183747-93183766 | None:intergenic | 35.0% | |
| GAAAAAATACCTCCGATAAG+TGG | + | chr3.3:93183842-93183861 | None:intergenic | 35.0% | |
| GAAATACAGAGATAGACACA+AGG | - | chr3.3:93182416-93182435 | MS.gene69404:CDS | 35.0% | |
| GGATAGGAGAATCTTATTGA+TGG | + | chr3.3:93182860-93182879 | None:intergenic | 35.0% | |
| GTGACACATCCAAGTTTAAT+TGG | + | chr3.3:93182616-93182635 | None:intergenic | 35.0% | |
| TAATGTAACAGCCTCTTTGA+CGG | + | chr3.3:93182455-93182474 | None:intergenic | 35.0% | |
| TCATTAATTCCACTTATCGG+AGG | - | chr3.3:93183830-93183849 | MS.gene69404:CDS | 35.0% | |
| TCCTCATCATCACTTGTTTA+TGG | - | chr3.3:93183305-93183324 | MS.gene69404:intron | 35.0% | |
| TCTTCTTCTTCCTAAGAGAA+GGG | + | chr3.3:93183805-93183824 | None:intergenic | 35.0% | |
| TGTGTCTATCTCTGTATTTC+TGG | + | chr3.3:93182416-93182435 | None:intergenic | 35.0% | |
| TTCTTCTTCTTCCTAAGAGA+AGG | + | chr3.3:93183806-93183825 | None:intergenic | 35.0% | |
| ! | ACCATAAACAAGTGATGATG+AGG | + | chr3.3:93183309-93183328 | None:intergenic | 35.0% |
| !! | TTCGGTGATAAAGTTTTAGC+TGG | - | chr3.3:93182475-93182494 | MS.gene69404:CDS | 35.0% |
| ACATTGTATGTCATCTCTCC+AGG | - | chr3.3:93183520-93183539 | MS.gene69404:intron | 40.0% | |
| ATGTTACCTCAAGGTCATCA+AGG | - | chr3.3:93183758-93183777 | MS.gene69404:CDS | 40.0% | |
| TAATGGCCAAGAGGAATTCT+TGG | - | chr3.3:93182814-93182833 | MS.gene69404:CDS | 40.0% | |
| TCTCATGTCATGTTACCTCA+AGG | - | chr3.3:93183749-93183768 | MS.gene69404:CDS | 40.0% | |
| TGCAACTAAACTCAAACACC+AGG | - | chr3.3:93182513-93182532 | MS.gene69404:CDS | 40.0% | |
| TGTCGTATATCACAGAACGT+AGG | + | chr3.3:93182674-93182693 | None:intergenic | 40.0% | |
| ! | ATGTGATGGTGTATAGAACC+TGG | + | chr3.3:93182534-93182553 | None:intergenic | 40.0% |
| !! | AAGGTGTTGAGAAGTTGGAA+AGG | - | chr3.3:93183777-93183796 | MS.gene69404:CDS | 40.0% |
| !! | TCATCAAGGTGTTGAGAAGT+TGG | - | chr3.3:93183772-93183791 | MS.gene69404:CDS | 40.0% |
| ATGACATGAGAAATGGACGG+TGG | + | chr3.3:93183740-93183759 | None:intergenic | 45.0% | |
| TGAAAACGTTACAGCTGAGC+TGG | - | chr3.3:93183445-93183464 | MS.gene69404:intron | 45.0% | |
| ! | ATGGGGTTGATCAGTCTTCA+AGG | - | chr3.3:93183420-93183439 | MS.gene69404:intron | 45.0% |
| ! | TCAACACCTTGATGACCTTG+AGG | + | chr3.3:93183767-93183786 | None:intergenic | 45.0% |
| ! | TGGGGTTGATCAGTCTTCAA+GGG | - | chr3.3:93183421-93183440 | MS.gene69404:intron | 45.0% |
| !! | CAAAATCGGTTTGTAAGGCG+AGG | + | chr3.3:93182724-93182743 | None:intergenic | 45.0% |
| !! | TATACTTATTAAAAAAATAA+TGG | - | chr3.3:93182797-93182816 | MS.gene69404:CDS | 5.0% |
| AAACGTTACAGCTGAGCTGG+AGG | - | chr3.3:93183448-93183467 | MS.gene69404:intron | 50.0% | |
| !! | TGGAAAGGCACCCTTCTCTT+AGG | - | chr3.3:93183792-93183811 | MS.gene69404:CDS | 50.0% |
| GCCATTCAGGTCCGTCAAAG+AGG | - | chr3.3:93182441-93182460 | MS.gene69404:CDS | 55.0% | |
| ! | GCCTCTTTGACGGACCTGAA+TGG | + | chr3.3:93182445-93182464 | None:intergenic | 55.0% |
| ! | TCGTGCGTTCGAGTTCTTCC+TGG | + | chr3.3:93183541-93183560 | None:intergenic | 55.0% |
| !! | TAGACACAAGGGCGCCATTC+AGG | - | chr3.3:93182428-93182447 | MS.gene69404:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 93182379 | 93183895 | 93182379 | ID=MS.gene69404 |
| chr3.3 | mRNA | 93182379 | 93183895 | 93182379 | ID=MS.gene69404.t1;Parent=MS.gene69404 |
| chr3.3 | exon | 93183740 | 93183895 | 93183740 | ID=MS.gene69404.t1.exon1;Parent=MS.gene69404.t1 |
| chr3.3 | CDS | 93183740 | 93183895 | 93183740 | ID=cds.MS.gene69404.t1;Parent=MS.gene69404.t1 |
| chr3.3 | exon | 93182379 | 93182876 | 93182379 | ID=MS.gene69404.t1.exon2;Parent=MS.gene69404.t1 |
| chr3.3 | CDS | 93182379 | 93182876 | 93182379 | ID=cds.MS.gene69404.t1;Parent=MS.gene69404.t1 |
| Gene Sequence |
| Protein sequence |
