AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69614

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69614.t1 MTR_5g028190 85.970 335 47 0 388 722 199 533 0.0 604
MS.gene69614.t1 MTR_5g028190 70.000 200 57 2 123 319 1 200 1.54e-83 275
MS.gene69614.t1 MTR_5g028130 76.471 340 76 2 388 724 1 339 0.0 524
MS.gene69614.t1 MTR_5g028930 42.420 686 299 18 61 721 2 616 2.60e-145 438
MS.gene69614.t1 MTR_5g028450 66.892 296 92 2 429 720 1 294 3.74e-129 385
MS.gene69614.t1 MTR_5g028110 71.728 191 36 1 534 724 4 176 8.40e-87 271
MS.gene69614.t1 MTR_5g091220 34.400 625 348 18 104 720 31 601 1.13e-80 269
MS.gene69614.t1 MTR_3g455790 29.654 607 360 14 104 703 33 579 1.25e-67 234
MS.gene69614.t1 MTR_3g055310 31.740 523 294 11 199 716 12 476 3.34e-66 227
MS.gene69614.t1 MTR_3g055310 33.008 512 280 12 210 716 48 501 6.26e-66 227
MS.gene69614.t1 MTR_3g055310 33.008 512 280 12 210 716 21 474 6.32e-66 226
MS.gene69614.t1 MTR_3g055310 33.763 465 253 9 252 716 39 448 1.48e-64 222
MS.gene69614.t1 MTR_3g055310 34.146 451 242 9 266 716 1 396 6.50e-64 219
MS.gene69614.t1 MTR_6g017215 57.609 184 78 0 532 715 6 189 6.97e-64 213
MS.gene69614.t1 MTR_2g438740 35.200 375 215 8 353 722 217 568 1.45e-63 222
MS.gene69614.t1 MTR_2g438740 31.953 169 101 7 103 269 41 197 3.95e-13 73.2
MS.gene69614.t1 MTR_5g028510 42.342 333 141 8 24 316 6 327 2.01e-63 215
MS.gene69614.t1 MTR_3g055310 40.767 287 159 3 427 713 55 330 3.21e-62 212
MS.gene69614.t1 MTR_5g028690 50.000 200 95 1 527 721 36 235 1.67e-59 201
MS.gene69614.t1 MTR_3g452020 27.742 620 382 15 104 714 33 595 1.07e-56 204
MS.gene69614.t1 MTR_2g438440 38.567 293 176 3 429 720 396 685 4.30e-56 204
MS.gene69614.t1 MTR_2g438400 36.949 295 178 4 429 720 255 544 3.14e-53 193
MS.gene69614.t1 MTR_2g438760 35.154 293 186 3 430 720 271 561 4.45e-50 185
MS.gene69614.t1 MTR_2g438760 26.054 261 167 9 53 311 5 241 1.69e-13 74.3
MS.gene69614.t1 MTR_2g438700 36.177 293 182 4 429 719 399 688 4.58e-50 187
MS.gene69614.t1 MTR_2g438720 36.242 298 177 6 429 720 401 691 8.49e-50 186
MS.gene69614.t1 MTR_2g438640 35.836 293 184 3 429 720 401 690 3.92e-48 181
MS.gene69614.t1 MTR_3g452020 31.250 320 214 4 385 702 88 403 1.17e-43 163
MS.gene69614.t1 MTR_5g028900 51.923 156 63 2 533 688 3 146 1.85e-43 154
MS.gene69614.t1 MTR_2g438640 33.788 293 164 3 429 720 401 664 1.15e-42 165
MS.gene69614.t1 MTR_2g020510 36.861 274 163 3 429 702 390 653 2.75e-42 164
MS.gene69614.t1 MTR_5g028840 45.699 186 92 1 532 717 2 178 2.75e-42 152
MS.gene69614.t1 MTR_5g028830 40.154 259 135 8 284 531 39 288 1.15e-41 154
MS.gene69614.t1 MTR_2g017930 25.705 638 393 18 139 723 37 646 1.39e-40 159
MS.gene69614.t1 MTR_3g058570 33.225 307 182 5 428 721 83 379 6.02e-39 149
MS.gene69614.t1 MTR_5g085360 26.279 723 394 27 107 713 23 722 6.99e-39 154
MS.gene69614.t1 MTR_2g020450 34.256 289 185 4 429 716 393 677 5.79e-37 148
MS.gene69614.t1 MTR_2g020450 28.846 208 131 7 102 303 4 200 2.18e-12 70.9
MS.gene69614.t1 MTR_2g020470 34.672 274 149 5 429 702 392 635 3.06e-36 145
MS.gene69614.t1 MTR_2g020470 26.887 212 134 8 102 305 4 202 9.55e-11 65.5
MS.gene69614.t1 MTR_3g050500 29.450 309 207 5 416 716 414 719 7.39e-33 135
MS.gene69614.t1 MTR_3g051060 28.328 293 205 3 430 718 435 726 1.57e-30 128
MS.gene69614.t1 MTR_3g051060 29.108 213 126 7 107 304 28 230 1.04e-13 75.1
MS.gene69614.t1 MTR_2g438640 36.842 190 118 2 532 720 399 587 3.21e-29 124
MS.gene69614.t1 MTR_5g028920 41.071 168 94 3 368 534 64 227 6.89e-29 116
MS.gene69614.t1 MTR_5g085350 31.507 292 194 4 429 716 437 726 7.12e-29 123
MS.gene69614.t1 MTR_5g085350 31.507 292 194 4 429 716 424 713 7.31e-29 123
MS.gene69614.t1 MTR_5g085340 30.068 296 201 3 429 719 443 737 4.83e-28 120
MS.gene69614.t1 MTR_1g113820 33.333 219 132 6 428 641 275 484 6.64e-23 103
MS.gene69614.t1 MTR_2g438470 40.000 135 78 2 474 608 366 497 1.79e-21 99.0
MS.gene69614.t1 MTR_1g113730 29.018 224 146 5 401 622 23 235 5.03e-19 90.1
MS.gene69614.t1 MTR_1g113760 25.287 261 183 5 420 674 231 485 6.26e-16 82.0
MS.gene69614.t1 MTR_6g016185 30.151 199 121 7 457 654 16 197 2.75e-15 75.5
MS.gene69614.t1 MTR_3g055750 28.972 214 138 6 104 314 33 235 3.42e-14 75.5
MS.gene69614.t1 MTR_3g058550 31.667 180 100 6 139 304 9 179 5.01e-13 69.3
MS.gene69614.t1 MTR_2g438580 39.474 76 45 1 546 621 2 76 4.04e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69614.t1 AT3G18670 35.861 633 354 15 104 724 50 642 4.08e-123 382
MS.gene69614.t1 AT3G18670 43.235 340 186 5 388 724 252 587 5.81e-87 286
MS.gene69614.t1 AT3G18670 34.450 209 117 7 104 303 50 247 4.37e-27 117
MS.gene69614.t1 AT5G04690 31.638 629 377 11 95 720 67 645 2.82e-85 283
MS.gene69614.t1 AT5G04700 30.979 623 378 11 103 720 80 655 4.10e-83 277
MS.gene69614.t1 AT5G04690 31.111 630 365 13 95 720 67 631 3.13e-80 269
MS.gene69614.t1 AT5G04680 30.408 638 399 17 103 720 67 679 1.42e-74 255
MS.gene69614.t1 AT5G04680 30.332 633 377 16 103 720 67 650 4.13e-73 250
MS.gene69614.t1 AT5G04730 31.100 582 338 14 151 720 58 588 3.22e-70 241
MS.gene69614.t1 AT5G04680 29.542 633 368 16 103 720 67 636 6.11e-70 241
MS.gene69614.t1 AT5G04680 31.294 572 327 16 164 720 7 527 1.03e-69 238
MS.gene69614.t1 AT5G35810 37.748 302 179 4 429 723 35 334 8.76e-62 211
MS.gene69614.t1 AT5G04690 31.471 537 333 13 199 720 95 611 4.43e-59 211
MS.gene69614.t1 AT5G04690 30.000 210 136 4 95 302 67 267 4.03e-17 85.9
MS.gene69614.t1 AT3G54065 47.093 172 85 1 518 683 8 179 2.83e-46 165
MS.gene69614.t1 AT5G04700 25.173 433 274 8 103 532 80 465 3.51e-33 134

Find 157 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCATGCACTTCTCTTTC+AGG 0.124188 5.3:+17565461 None:intergenic
TTGCACTTGGCAGGATATTT+AGG 0.127030 5.3:-17564438 MS.gene69614:CDS
CCAAAGAATTCAAGAGTTTA+AGG 0.157617 5.3:-17564715 MS.gene69614:CDS
AACTTACCCAACTGCTTTAC+TGG 0.201955 5.3:+17565216 None:intergenic
TGTATCACTGCAGAAATATT+TGG 0.219312 5.3:-17566423 MS.gene69614:intron
TTCACTTGCTATGCTCTTAA+AGG 0.220345 5.3:-17563430 MS.gene69614:CDS
CCTTAAACTCTTGAATTCTT+TGG 0.238324 5.3:+17564715 None:intergenic
TAAAGAAGCCTGGAATAAAT+TGG 0.241875 5.3:-17567241 MS.gene69614:CDS
ATACATGATGCAACTGTAAA+TGG 0.242921 5.3:-17564510 MS.gene69614:CDS
TTAGAACGCGCACCAATTTC+AGG 0.251761 5.3:+17567369 None:intergenic
CTTCGCTATATTCGTGTTTC+CGG 0.254247 5.3:+17566641 None:intergenic
AATATATTCCACATCCTCTA+TGG 0.260429 5.3:-17566802 MS.gene69614:CDS
CACTAGCTCCTTGTGGCTTT+CGG 0.282604 5.3:+17563791 None:intergenic
GTGACCTCTATTCCTGAAAT+TGG 0.292763 5.3:-17567381 MS.gene69614:CDS
TGCACTTGGCAGGATATTTA+GGG 0.308043 5.3:-17564437 MS.gene69614:CDS
TTTCCAAGTGGTAGTGGATA+TGG 0.311953 5.3:-17566208 MS.gene69614:CDS
ACTTCGGTCCTTCTCTTCAT+TGG 0.319322 5.3:-17563570 MS.gene69614:CDS
AATTGTATCCATCAGGAATA+AGG 0.321064 5.3:-17564797 MS.gene69614:intron
CTTCACGTTGCGGTAGTTGC+TGG 0.322177 5.3:-17566768 MS.gene69614:CDS
CTAAGACTGAATATGAGATT+TGG 0.338368 5.3:-17567350 MS.gene69614:CDS
AGGGTAATCCTGACCTCTTA+TGG 0.352153 5.3:-17564605 MS.gene69614:CDS
CCAAAGACAACTACGAAAAC+TGG 0.369243 5.3:-17567452 MS.gene69614:CDS
CGAGGAAATTGTGAAGAAAT+TGG 0.372095 5.3:-17566742 MS.gene69614:CDS
ATGCAAACTCGTTTATTAAG+AGG 0.378242 5.3:-17566836 MS.gene69614:CDS
AACAGGGATTTCACCATCAT+TGG 0.379563 5.3:+17566560 None:intergenic
TGTTCTTCTTGCTGCTGCTA+AGG 0.379583 5.3:-17566541 MS.gene69614:CDS
TCACATGTCATTTCAGAATT+CGG 0.380455 5.3:-17564963 MS.gene69614:CDS
AATAAGTAACAAGTTTCAAT+TGG 0.387620 5.3:+17563296 None:intergenic
AGTTGCACCCAATTTATTCC+AGG 0.399506 5.3:+17567233 None:intergenic
GCTGCAGCTTTCACTGTCCC+TGG 0.408383 5.3:-17563690 MS.gene69614:CDS
TTAGCAGCAGCAAGAAGAAC+AGG 0.410668 5.3:+17566543 None:intergenic
TAGAAACCACAGAGAAGAAA+AGG 0.416389 5.3:+17563472 None:intergenic
TTCCAAGTGGTAGTGGATAT+GGG 0.416844 5.3:-17566207 MS.gene69614:CDS
AAATGCCGTGTAGACGCATT+TGG 0.419337 5.3:-17564471 MS.gene69614:CDS
AATTCCATGTTGCACTTGGC+AGG 0.419647 5.3:-17564447 MS.gene69614:CDS
ATCAGAACTTAGAGAGGCTT+CGG 0.421195 5.3:-17564691 MS.gene69614:CDS
GGGTAATCCTGACCTCTTAT+GGG 0.423361 5.3:-17564604 MS.gene69614:CDS
CTAATATGAGTAAGATTCTC+TGG 0.424630 5.3:+17567279 None:intergenic
TCACTTGCTATGCTCTTAAA+GGG 0.427681 5.3:-17563429 MS.gene69614:CDS
CATTGGTCTTCATAGATAAC+AGG 0.427879 5.3:+17566577 None:intergenic
ACCTTAGTGGCAATGACTTA+AGG 0.429666 5.3:+17563514 None:intergenic
AGATGCAAAGAGAAATTCTA+TGG 0.430747 5.3:-17564374 MS.gene69614:CDS
TTCACGTTGCGGTAGTTGCT+GGG 0.432176 5.3:-17566767 MS.gene69614:CDS
TCTCTTTGCATCTGCATAGC+AGG 0.438079 5.3:+17564384 None:intergenic
GAGATTTGGAACAGGAAGAA+TGG 0.441267 5.3:-17567336 MS.gene69614:CDS
CCACAAATCGCACTTTGAAT+CGG 0.442750 5.3:-17566275 MS.gene69614:CDS
TCTGTGTTTCCAAGTGGTAG+TGG 0.443413 5.3:-17566214 MS.gene69614:CDS
CTGAATATGAGATTTGGAAC+AGG 0.444534 5.3:-17567344 MS.gene69614:CDS
GTTCTTCTTGCTGCTGCTAA+GGG 0.445513 5.3:-17566540 MS.gene69614:CDS
TCGGTACTATTAAAGTTCTC+TGG 0.448532 5.3:-17564944 MS.gene69614:intron
GACGGAACTAGAACGATAAC+AGG 0.451356 5.3:+17563363 None:intergenic
CGATTCAAAGTGCGATTTGT+GGG 0.457581 5.3:+17566276 None:intergenic
TATAGCGAAGTGTATGACTA+TGG 0.458675 5.3:-17566628 MS.gene69614:CDS
TCCTTAAGTCATTGCCACTA+AGG 0.460081 5.3:-17563515 MS.gene69614:CDS
TCTTGTGGGACATGGTCTGT+GGG 0.464135 5.3:-17567409 MS.gene69614:CDS
TCAATGGCCCATAAGAGGTC+AGG 0.465739 5.3:+17564597 None:intergenic
TTTCAGCGTAACGAGCCGTT+AGG 0.467061 5.3:+17563544 None:intergenic
GTTTATAGATGCAATGAGAA+AGG 0.467103 5.3:-17564625 MS.gene69614:CDS
CTTGTTAGAAACTATCTTGT+GGG 0.467922 5.3:-17567423 MS.gene69614:CDS
ATGCGTGCCAATGCGTATAA+TGG 0.471182 5.3:+17566241 None:intergenic
TTGTGGGCAAGAGGCATTTG+AGG 0.474333 5.3:+17566292 None:intergenic
TGTGCAGCGATTTATATTGA+AGG 0.474499 5.3:+17566886 None:intergenic
TTCATATTGCTAAAGAAGCC+TGG 0.477292 5.3:-17567251 MS.gene69614:CDS
AAAGAAGCCTGGAATAAATT+GGG 0.481257 5.3:-17567240 MS.gene69614:intron
AGCTGGTTCATTTACTCTTG+TGG 0.482801 5.3:-17563739 MS.gene69614:CDS
AGATTTGGAACAGGAAGAAT+GGG 0.485550 5.3:-17567335 MS.gene69614:CDS
TCAGCTCAGCAACAAGAGCT+AGG 0.486371 5.3:+17566664 None:intergenic
CATAGCAAGTGAAGCACAGA+AGG 0.486804 5.3:+17563440 None:intergenic
ATCTTGTGGGACATGGTCTG+TGG 0.486835 5.3:-17567410 MS.gene69614:CDS
GTGAAGAAATTGGTGAAGGA+AGG 0.488215 5.3:-17566732 MS.gene69614:CDS
AATTGTGCACCCCAAGTGTA+AGG 0.491201 5.3:-17563856 MS.gene69614:CDS
TTGCATCTGCATAGCAGGAC+CGG 0.495069 5.3:+17564389 None:intergenic
ATATATTCCACATCCTCTAT+GGG 0.496055 5.3:-17566801 MS.gene69614:CDS
TTGTCTTGATTGTTTCCACC+AGG 0.496117 5.3:+17563672 None:intergenic
TTGTACACCGTTGGAAGACC+TGG 0.502955 5.3:-17566487 MS.gene69614:CDS
TTTATAGATGCAATGAGAAA+GGG 0.503030 5.3:-17564624 MS.gene69614:CDS
ATCATGTATGAGTTCGAAAA+CGG 0.503307 5.3:+17564524 None:intergenic
TGCGATAATTATCATCGGCC+AGG 0.509460 5.3:+17566469 None:intergenic
TGGCAATTCCATGTTGCACT+TGG 0.511014 5.3:-17564451 MS.gene69614:CDS
TTGTGAAGCAGTAGAATGAC+AGG 0.514230 5.3:-17567516 None:intergenic
TCTTTACCGTTGTACACCGT+TGG 0.515694 5.3:-17566496 MS.gene69614:CDS
TCTGATCTTGATCGTAGATC+CGG 0.515841 5.3:-17564408 MS.gene69614:CDS
CCGATTCAAAGTGCGATTTG+TGG 0.519858 5.3:+17566275 None:intergenic
CGCACCAATTTCAGGAATAG+AGG 0.521001 5.3:+17567377 None:intergenic
CTTTAGGAAGGTTATGTGGA+CGG 0.525505 5.3:-17565254 MS.gene69614:intron
CACAAGGAGCTAGTGAAAGC+TGG 0.525545 5.3:-17563783 MS.gene69614:CDS
GCTCTTGTTGCTGAGCTGAC+CGG 0.526277 5.3:-17566660 MS.gene69614:CDS
TGCAAACTCGTTTATTAAGA+GGG 0.527286 5.3:-17566835 MS.gene69614:CDS
TCTTGCAAATATTGCCAAAG+AGG 0.534735 5.3:-17565420 MS.gene69614:CDS
TGTGATGTCGTGCAAGAACA+AGG 0.535047 5.3:+17563641 None:intergenic
GAGGCATTTGAGGAAATCGT+TGG 0.537554 5.3:+17566302 None:intergenic
GGTCCTCCCCATAGAGGATG+TGG 0.539677 5.3:+17566794 None:intergenic
AAGAGAAATTCTATGGTTCA+AGG 0.541072 5.3:-17564367 MS.gene69614:intron
TCGTAGTTGTCTTTGGTCAG+TGG 0.542773 5.3:+17567459 None:intergenic
TGTGCAACCATTATACGCAT+TGG 0.543058 5.3:-17566248 MS.gene69614:CDS
AGTGCGAAAAGTAACCTTAG+TGG 0.548121 5.3:+17563501 None:intergenic
CAACACAAAGCTTCTTTCGT+AGG 0.550008 5.3:-17565392 MS.gene69614:intron
ATGTTACAAGCAGCAAAGCA+TGG 0.552695 5.3:-17564657 MS.gene69614:CDS
CAGCTTTCACTAGCTCCTTG+TGG 0.553008 5.3:+17563784 None:intergenic
TTATCGCCAGTAAAGCAGTT+GGG 0.556264 5.3:-17565222 MS.gene69614:intron
AACCCATATCCACTACCACT+TGG 0.557493 5.3:+17566205 None:intergenic
TCTTGTTAGAAACTATCTTG+TGG 0.559427 5.3:-17567424 MS.gene69614:CDS
AGAAACTATCTTGTGGGACA+TGG 0.562860 5.3:-17567417 MS.gene69614:CDS
AAATGGGCAAAAGATACAGC+TGG 0.563052 5.3:-17563756 MS.gene69614:CDS
AGAAACCACAGAGAAGAAAA+GGG 0.563826 5.3:+17563473 None:intergenic
GCTTTAATTTGCAGGTGTGG+TGG 0.564757 5.3:-17566919 MS.gene69614:intron
ACTTGAAAATCTCAAGGAAG+AGG 0.567742 5.3:+17563331 None:intergenic
TAAGGAATCAGAACTTAGAG+AGG 0.568613 5.3:-17564697 MS.gene69614:CDS
GTTTCTTTAGGAAGGTTATG+TGG 0.570625 5.3:-17565258 MS.gene69614:intron
GCCGATGATAATTATCGCAA+TGG 0.574637 5.3:-17566465 MS.gene69614:CDS
TGAAGAAATTGGTGAAGGAA+GGG 0.574748 5.3:-17566731 MS.gene69614:CDS
TTTATCGCCAGTAAAGCAGT+TGG 0.576962 5.3:-17565223 MS.gene69614:CDS
CAGCTCAGCAACAAGAGCTA+GGG 0.580236 5.3:+17566665 None:intergenic
AGGAAGAGGCGCAACTGTGA+CGG 0.582572 5.3:+17563345 None:intergenic
TTATCTATGAAGACCAATGA+TGG 0.582929 5.3:-17566573 MS.gene69614:CDS
TGTTACAAGCAGCAAAGCAT+GGG 0.588268 5.3:-17564656 MS.gene69614:CDS
TGCGGTAGTTGCTGGGCACG+AGG 0.589759 5.3:-17566760 MS.gene69614:CDS
TAAGAAAATAACACGGTCAG+AGG 0.590331 5.3:-17566601 MS.gene69614:CDS
TCTCTTCATTGGAATCCTAA+CGG 0.591072 5.3:-17563559 MS.gene69614:CDS
CTGTAGACTTGAAAATCTCA+AGG 0.594097 5.3:+17563325 None:intergenic
GCAGCTTTCACTGTCCCTGG+TGG 0.601445 5.3:-17563687 MS.gene69614:CDS
AAGTGCGATTTGTGGGCAAG+AGG 0.604929 5.3:+17566283 None:intergenic
GGAACAAGCATTGCAGCGAG+TGG 0.605832 5.3:-17567495 MS.gene69614:CDS
AGTATATTGAGAGCGGTGAG+TGG 0.609550 5.3:-17566863 MS.gene69614:CDS
ACCATTGCGATAATTATCAT+CGG 0.609954 5.3:+17566464 None:intergenic
TAGAACGATAACAGGAACAC+TGG 0.611619 5.3:+17563371 None:intergenic
AACTACCGCAACGTGAAGAA+CGG 0.612033 5.3:+17566773 None:intergenic
AATTGCCAAATGCGTCTACA+CGG 0.612652 5.3:+17564466 None:intergenic
TTAGGATTCCAATGAAGAGA+AGG 0.614556 5.3:+17563562 None:intergenic
ATGCCTTCTGTGTTTCCAAG+TGG 0.615562 5.3:-17566220 MS.gene69614:CDS
TGCAACTGTAAATGGACGGA+AGG 0.618113 5.3:-17564502 MS.gene69614:CDS
CTTGTGGGACATGGTCTGTG+GGG 0.619674 5.3:-17567408 MS.gene69614:CDS
CTACCACTTGGAAACACAGA+AGG 0.622603 5.3:+17566217 None:intergenic
AAGAACGGTCCTCCCCATAG+AGG 0.622685 5.3:+17566788 None:intergenic
AATTGTGAAGAAATTGGTGA+AGG 0.625256 5.3:-17566736 MS.gene69614:CDS
ATATCCTGCCAAGTGCAACA+TGG 0.627952 5.3:+17564443 None:intergenic
GGTGGAAACAATCAAGACAA+TGG 0.629811 5.3:-17563669 MS.gene69614:CDS
ATGATGCAACTGTAAATGGA+CGG 0.638038 5.3:-17564506 MS.gene69614:CDS
CATATCATTCAACTAACATG+TGG 0.643061 5.3:-17567303 MS.gene69614:CDS
GATATTTACCGAAAGCCACA+AGG 0.651211 5.3:-17563799 MS.gene69614:CDS
CTTTCGGTAAATATCTCACG+AGG 0.651762 5.3:+17563807 None:intergenic
TGTCTTGATTGTTTCCACCA+GGG 0.652150 5.3:+17563673 None:intergenic
GAGGACCGTTCTTCACGTTG+CGG 0.655029 5.3:-17566778 MS.gene69614:CDS
GTGATGTCGTGCAAGAACAA+GGG 0.659099 5.3:+17563642 None:intergenic
ATTCCACATCCTCTATGGGG+AGG 0.660094 5.3:-17566797 MS.gene69614:CDS
TAGCAGCAGCAAGAAGAACA+GGG 0.665135 5.3:+17566544 None:intergenic
TTGGAACAGGAAGAATGGGA+AGG 0.668429 5.3:-17567331 MS.gene69614:CDS
TATTTCAAGAGCTACACGGT+AGG 0.669157 5.3:+17564752 None:intergenic
GTGAAAATGAAAGACAATCG+AGG 0.669964 5.3:-17566696 MS.gene69614:CDS
TCATCGGCCAGGTCTTCCAA+CGG 0.671668 5.3:+17566480 None:intergenic
CTACGATCAAGATCAGAGGA+AGG 0.678354 5.3:+17564414 None:intergenic
CTGCACAAGTATATTGAGAG+CGG 0.680709 5.3:-17566870 MS.gene69614:CDS
GGATCTACGATCAAGATCAG+AGG 0.680818 5.3:+17564410 None:intergenic
TCAATATTTCAAGAGCTACA+CGG 0.684421 5.3:+17564748 None:intergenic
GGTCTTCCAACGGTGTACAA+CGG 0.687225 5.3:+17566490 None:intergenic
TATATTCCACATCCTCTATG+GGG 0.688060 5.3:-17566800 MS.gene69614:CDS
TGGTATATAAGAAAATAACA+CGG 0.754492 5.3:-17566608 MS.gene69614:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTTATAAAAGAAATTTAA+AGG - chr5.3:17564863-17564882 MS.gene69614:intron 10.0%
!! CTTAAATACTTATAATATTA+GGG - chr5.3:17563808-17563827 MS.gene69614:CDS 10.0%
!! TCTTAAATACTTATAATATT+AGG - chr5.3:17563807-17563826 MS.gene69614:CDS 10.0%
!! AAACTCCTTTAATATATTAT+TGG + chr5.3:17565000-17565019 None:intergenic 15.0%
!! ATTTAAAGGTGATTTATAAA+AGG - chr5.3:17564877-17564896 MS.gene69614:intron 15.0%
!! TAATTAATTAATCACAAAGA+GGG + chr5.3:17565976-17565995 None:intergenic 15.0%
!! TACTTCCAATAATATATTAA+AGG - chr5.3:17564992-17565011 MS.gene69614:intron 15.0%
!! TTAATTAATTAATCACAAAG+AGG + chr5.3:17565977-17565996 None:intergenic 15.0%
!! TTTAAAGGTGATTTATAAAA+GGG - chr5.3:17564878-17564897 MS.gene69614:intron 15.0%
!!! AAAAACTAAATTTGAAATTC+TGG - chr5.3:17566734-17566753 MS.gene69614:CDS 15.0%
!!! ACTGAATTTTAATTAAATTG+AGG - chr5.3:17564421-17564440 MS.gene69614:CDS 15.0%
!!! CTGAATTTTAATTAAATTGA+GGG - chr5.3:17564422-17564441 MS.gene69614:CDS 15.0%
!!! TTACACATTTTTTTTTTTTC+AGG - chr5.3:17565808-17565827 MS.gene69614:intron 15.0%
!! AAACTATGTCTAATATGTAT+AGG + chr5.3:17563710-17563729 None:intergenic 20.0%
!! AACTATGTCTAATATGTATA+GGG + chr5.3:17563709-17563728 None:intergenic 20.0%
!! AATTAATTAATCACAAAGAG+GGG + chr5.3:17565975-17565994 None:intergenic 20.0%
!! ATACATGACATTTAAATAAG+TGG - chr5.3:17566797-17566816 MS.gene69614:CDS 20.0%
!! ATGAATAAGATAATAATGTG+TGG + chr5.3:17566855-17566874 None:intergenic 20.0%
!! CAATTAGTAAGGAAAATAAA+TGG - chr5.3:17563833-17563852 MS.gene69614:CDS 20.0%
!! CTAATATACCTATTTAAACA+AGG + chr5.3:17565909-17565928 None:intergenic 20.0%
!! TACACATTAATCTATTCTTT+GGG - chr5.3:17563667-17563686 MS.gene69614:CDS 20.0%
!! TAGTTTCTAAATGTATTGAA+AGG - chr5.3:17563724-17563743 MS.gene69614:CDS 20.0%
!! TGGTATATAAGAAAATAACA+CGG - chr5.3:17564204-17564223 MS.gene69614:intron 20.0%
!!! CATTTATTTTCCTTACTAAT+TGG + chr5.3:17563835-17563854 None:intergenic 20.0%
!!! TTGTTAATCCTTGTTTAAAT+AGG - chr5.3:17565898-17565917 MS.gene69614:intron 20.0%
! AATGAATACTATATGTGTAG+TGG + chr5.3:17564962-17564981 None:intergenic 25.0%
! AATGTCATGTATCTCAAAAT+TGG + chr5.3:17566789-17566808 None:intergenic 25.0%
! AATTTGAAAAGTAAGCGAAA+CGG + chr5.3:17564694-17564713 None:intergenic 25.0%
! AGACCAACTTTACAAAATTA+AGG - chr5.3:17565156-17565175 MS.gene69614:intron 25.0%
! ATCAACACACATATATAACA+GGG + chr5.3:17565774-17565793 None:intergenic 25.0%
! CTGAAGATTATTAATCTTCT+TGG - chr5.3:17565529-17565548 MS.gene69614:intron 25.0%
! GAATGATTGTACTTACAATA+AGG + chr5.3:17565950-17565969 None:intergenic 25.0%
! GAGTAAATAATGAATGAGAT+TGG - chr5.3:17565224-17565243 MS.gene69614:CDS 25.0%
! GCCATTGATAAAAATAAAAG+AGG - chr5.3:17566230-17566249 MS.gene69614:CDS 25.0%
! GTACACATTAATCTATTCTT+TGG - chr5.3:17563666-17563685 MS.gene69614:CDS 25.0%
! TATCAACACACATATATAAC+AGG + chr5.3:17565775-17565794 None:intergenic 25.0%
! TCGATTCAATATTGTATCTT+AGG - chr5.3:17564469-17564488 MS.gene69614:CDS 25.0%
! TGCAAACTTATAATGTTACA+CGG - chr5.3:17564753-17564772 MS.gene69614:CDS 25.0%
! TTTATAGATGCAATGAGAAA+GGG - chr5.3:17566188-17566207 MS.gene69614:CDS 25.0%
!! ACTCCTTAATTTTGTAAAGT+TGG + chr5.3:17565162-17565181 None:intergenic 25.0%
!! CTTATTGTGAAATTTTGTGT+AGG - chr5.3:17566923-17566942 MS.gene69614:intron 25.0%
!! GTTTGTATTTTATAAGACAC+CGG + chr5.3:17564913-17564932 None:intergenic 25.0%
!! GTTTTTCTACAATATGCAAA+TGG - chr5.3:17565501-17565520 MS.gene69614:intron 25.0%
!! TATATTTTCCTTATTCCTGA+TGG + chr5.3:17566026-17566045 None:intergenic 25.0%
!!! GCCTCTTTTATTTTTATCAA+TGG + chr5.3:17566234-17566253 None:intergenic 25.0%
!!! TTTCGCTTACTTTTCAAATT+GGG - chr5.3:17564694-17564713 MS.gene69614:CDS 25.0%
AAAGAAGCCTGGAATAAATT+GGG - chr5.3:17563572-17563591 MS.gene69614:CDS 30.0%
AAGAGAAATTCTATGGTTCA+AGG - chr5.3:17566445-17566464 MS.gene69614:CDS 30.0%
AATATATTCCACATCCTCTA+TGG - chr5.3:17564010-17564029 MS.gene69614:intron 30.0%
AATTGTATCCATCAGGAATA+AGG - chr5.3:17566015-17566034 MS.gene69614:intron 30.0%
AATTGTGAAGAAATTGGTGA+AGG - chr5.3:17564076-17564095 MS.gene69614:intron 30.0%
ACCATTGCGATAATTATCAT+CGG + chr5.3:17564351-17564370 None:intergenic 30.0%
AGATGCAAAGAGAAATTCTA+TGG - chr5.3:17566438-17566457 MS.gene69614:CDS 30.0%
ATACATGATGCAACTGTAAA+TGG - chr5.3:17566302-17566321 MS.gene69614:CDS 30.0%
ATATATTCCACATCCTCTAT+GGG - chr5.3:17564011-17564030 MS.gene69614:intron 30.0%
ATATTAGGGTCCAATTAGTA+AGG - chr5.3:17563822-17563841 MS.gene69614:CDS 30.0%
ATCATGTATGAGTTCGAAAA+CGG + chr5.3:17566291-17566310 None:intergenic 30.0%
ATGCAAACTCGTTTATTAAG+AGG - chr5.3:17563976-17563995 MS.gene69614:intron 30.0%
CATATCATTCAACTAACATG+TGG - chr5.3:17563509-17563528 MS.gene69614:CDS 30.0%
CCAAAGAATTCAAGAGTTTA+AGG - chr5.3:17566097-17566116 MS.gene69614:intron 30.0%
CCTTAAACTCTTGAATTCTT+TGG + chr5.3:17566100-17566119 None:intergenic 30.0%
CTAAGACTGAATATGAGATT+TGG - chr5.3:17563462-17563481 MS.gene69614:CDS 30.0%
CTAATATGAGTAAGATTCTC+TGG + chr5.3:17563536-17563555 None:intergenic 30.0%
CTTGTTAGAAACTATCTTGT+GGG - chr5.3:17563389-17563408 MS.gene69614:CDS 30.0%
GGTAATCTCAAAACTAAAAG+CGG + chr5.3:17565333-17565352 None:intergenic 30.0%
GTGGAGATGTTAAATTATGT+TGG + chr5.3:17564943-17564962 None:intergenic 30.0%
GTTAGTTGTGAAGTTGTTAT+AGG + chr5.3:17566711-17566730 None:intergenic 30.0%
GTTTATAGATGCAATGAGAA+AGG - chr5.3:17566187-17566206 MS.gene69614:CDS 30.0%
TAAAGAAGCCTGGAATAAAT+TGG - chr5.3:17563571-17563590 MS.gene69614:CDS 30.0%
TACATGATCTGTTTGGAAAT+TGG - chr5.3:17565040-17565059 MS.gene69614:intron 30.0%
TATAAAAGGGAAATGCTAAC+CGG - chr5.3:17564891-17564910 MS.gene69614:intron 30.0%
TATATACCTTGCTCAATATC+TGG + chr5.3:17563629-17563648 None:intergenic 30.0%
TCAATATTGTATCTTAGGTG+TGG - chr5.3:17564474-17564493 MS.gene69614:CDS 30.0%
TCAATATTTCAAGAGCTACA+CGG + chr5.3:17566067-17566086 None:intergenic 30.0%
TCTTGTTAGAAACTATCTTG+TGG - chr5.3:17563388-17563407 MS.gene69614:CDS 30.0%
TGCAAACTCGTTTATTAAGA+GGG - chr5.3:17563977-17563996 MS.gene69614:intron 30.0%
TGTATCACTGCAGAAATATT+TGG - chr5.3:17564389-17564408 MS.gene69614:CDS 30.0%
TTATCTATGAAGACCAATGA+TGG - chr5.3:17564239-17564258 MS.gene69614:intron 30.0%
TTGGGTCATCTTAAAATTCT+TGG - chr5.3:17564712-17564731 MS.gene69614:CDS 30.0%
TTTCACAATAAGTTATAGCG+CGG + chr5.3:17566915-17566934 None:intergenic 30.0%
! AGAGTTTTACATGATCTGTT+TGG - chr5.3:17565033-17565052 MS.gene69614:intron 30.0%
! AGTGAAGTTTGATGAGTTTT+TGG + chr5.3:17565113-17565132 None:intergenic 30.0%
! TCACATGTCATTTCAGAATT+CGG - chr5.3:17565849-17565868 MS.gene69614:intron 30.0%
! TCTTTTCACTTCTTCAACTT+CGG - chr5.3:17567226-17567245 MS.gene69614:intron 30.0%
! TTATTCCTGATGGATACAAT+TGG + chr5.3:17566016-17566035 None:intergenic 30.0%
! TTTAAGTGTCTTTTGTGAGT+GGG - chr5.3:17563758-17563777 MS.gene69614:CDS 30.0%
!! AAGCTCTAGAAAGAAGAATT+AGG + chr5.3:17565462-17565481 None:intergenic 30.0%
!! ACATACATGCTTTAATTTGC+AGG - chr5.3:17563885-17563904 MS.gene69614:intron 30.0%
!! AGAGAAGTGCATGAATTTTA+CGG - chr5.3:17565357-17565376 MS.gene69614:intron 30.0%
!! ATCTTCTTGGTTGTTTCTTT+AGG - chr5.3:17565542-17565561 MS.gene69614:intron 30.0%
!! GAGTATCGTTCCAATTTTAT+TGG - chr5.3:17565178-17565197 MS.gene69614:intron 30.0%
!! GTTTCGCTTACTTTTCAAAT+TGG - chr5.3:17564693-17564712 MS.gene69614:CDS 30.0%
!! TTTTAAGTGTCTTTTGTGAG+TGG - chr5.3:17563757-17563776 MS.gene69614:CDS 30.0%
!!! ATTTTTTGCTTCCTTACACT+TGG + chr5.3:17566970-17566989 None:intergenic 30.0%
!!! TTTGTGTTGTTTTTCCTCTT+TGG + chr5.3:17565409-17565428 None:intergenic 30.0%
!!! TTTTTTGCTTCCTTACACTT+GGG + chr5.3:17566969-17566988 None:intergenic 30.0%
AAAGATCACAGATCACAACA+AGG + chr5.3:17565677-17565696 None:intergenic 35.0%
AAGATCACAGATCACAACAA+GGG + chr5.3:17565676-17565695 None:intergenic 35.0%
AATTCATGCACTTCTCTTTC+AGG + chr5.3:17565354-17565373 None:intergenic 35.0%
ACTTGAAAATCTCAAGGAAG+AGG + chr5.3:17567484-17567503 None:intergenic 35.0%
AGAAACCACAGAGAAGAAAA+GGG + chr5.3:17567342-17567361 None:intergenic 35.0%
AGTGAAAGCTGGAGAAAAAT+GGG - chr5.3:17567040-17567059 MS.gene69614:intron 35.0%
ATGATGCAACTGTAAATGGA+CGG - chr5.3:17566306-17566325 MS.gene69614:CDS 35.0%
CGAGGAAATTGTGAAGAAAT+TGG - chr5.3:17564070-17564089 MS.gene69614:intron 35.0%
CTGTAGACTTGAAAATCTCA+AGG + chr5.3:17567490-17567509 None:intergenic 35.0%
GAGAAGAGATTAGAGAGTTT+AGG - chr5.3:17566585-17566604 MS.gene69614:CDS 35.0%
GCTTATATGTTGGAACAAAG+AGG - chr5.3:17566524-17566543 MS.gene69614:CDS 35.0%
GTGAAAATGAAAGACAATCG+AGG - chr5.3:17564116-17564135 MS.gene69614:intron 35.0%
TAAGAAAATAACACGGTCAG+AGG - chr5.3:17564211-17564230 MS.gene69614:intron 35.0%
TAAGGAATCAGAACTTAGAG+AGG - chr5.3:17566115-17566134 MS.gene69614:intron 35.0%
TAATTAATCACAAAGAGGGG+AGG + chr5.3:17565972-17565991 None:intergenic 35.0%
TAGAAACCACAGAGAAGAAA+AGG + chr5.3:17567343-17567362 None:intergenic 35.0%
TAGTGAAAGCTGGAGAAAAA+TGG - chr5.3:17567039-17567058 MS.gene69614:intron 35.0%
TATAGCGAAGTGTATGACTA+TGG - chr5.3:17564184-17564203 MS.gene69614:intron 35.0%
TATATTCCACATCCTCTATG+GGG - chr5.3:17564012-17564031 MS.gene69614:intron 35.0%
TCGGTACTATTAAAGTTCTC+TGG - chr5.3:17565868-17565887 MS.gene69614:intron 35.0%
TCTCTTCATTGGAATCCTAA+CGG - chr5.3:17567253-17567272 MS.gene69614:CDS 35.0%
TCTTGCAAATATTGCCAAAG+AGG - chr5.3:17565392-17565411 MS.gene69614:intron 35.0%
TGAAGAAATTGGTGAAGGAA+GGG - chr5.3:17564081-17564100 MS.gene69614:intron 35.0%
TGTGCAGCGATTTATATTGA+AGG + chr5.3:17563929-17563948 None:intergenic 35.0%
TTAGGATTCCAATGAAGAGA+AGG + chr5.3:17567253-17567272 None:intergenic 35.0%
TTCATATTGCTAAAGAAGCC+TGG - chr5.3:17563561-17563580 MS.gene69614:CDS 35.0%
! AGATTTGGAACAGGAAGAAT+GGG - chr5.3:17563477-17563496 MS.gene69614:CDS 35.0%
! CTGAATATGAGATTTGGAAC+AGG - chr5.3:17563468-17563487 MS.gene69614:CDS 35.0%
! GTTTCTTTAGGAAGGTTATG+TGG - chr5.3:17565554-17565573 MS.gene69614:intron 35.0%
! TTTTATCAATGGCCCATAAG+AGG + chr5.3:17566223-17566242 None:intergenic 35.0%
! TTTTTGCTTCCTTACACTTG+GGG + chr5.3:17566968-17566987 None:intergenic 35.0%
!! CATTGGTCTTCATAGATAAC+AGG + chr5.3:17564238-17564257 None:intergenic 35.0%
!! GAGAAGTGCATGAATTTTAC+GGG - chr5.3:17565358-17565377 MS.gene69614:intron 35.0%
!! TCACTTGCTATGCTCTTAAA+GGG - chr5.3:17567383-17567402 MS.gene69614:CDS 35.0%
!! TCTTGGTTGTTTCTTTAGGA+AGG - chr5.3:17565546-17565565 MS.gene69614:intron 35.0%
!! TTCACTTGCTATGCTCTTAA+AGG - chr5.3:17567382-17567401 MS.gene69614:CDS 35.0%
AAATGGGCAAAAGATACAGC+TGG - chr5.3:17567056-17567075 MS.gene69614:intron 40.0%
AACAGGGATTTCACCATCAT+TGG + chr5.3:17564255-17564274 None:intergenic 40.0%
AACTTACCCAACTGCTTTAC+TGG + chr5.3:17565599-17565618 None:intergenic 40.0%
AATTGCCAAATGCGTCTACA+CGG + chr5.3:17566349-17566368 None:intergenic 40.0%
ACCTTGCTCAATATCTGGAT+CGG + chr5.3:17563624-17563643 None:intergenic 40.0%
AGAAACTATCTTGTGGGACA+TGG - chr5.3:17563395-17563414 MS.gene69614:CDS 40.0%
AGTGCGAAAAGTAACCTTAG+TGG + chr5.3:17567314-17567333 None:intergenic 40.0%
AGTTGCACCCAATTTATTCC+AGG + chr5.3:17563582-17563601 None:intergenic 40.0%
ATCAGAACTTAGAGAGGCTT+CGG - chr5.3:17566121-17566140 MS.gene69614:intron 40.0%
ATGTTACAAGCAGCAAAGCA+TGG - chr5.3:17566155-17566174 MS.gene69614:intron 40.0%
CAAAGCTTCTTTCGTAGGTA+TGG - chr5.3:17565425-17565444 MS.gene69614:CDS 40.0%
CAACACAAAGCTTCTTTCGT+AGG - chr5.3:17565420-17565439 MS.gene69614:CDS 40.0%
CACACATATATAACAGGGAC+TGG + chr5.3:17565769-17565788 None:intergenic 40.0%
CATGCATGCAGCTTATATGT+TGG - chr5.3:17566514-17566533 MS.gene69614:CDS 40.0%
CCAAAGACAACTACGAAAAC+TGG - chr5.3:17563360-17563379 MS.gene69614:CDS 40.0%
CCACAAATCGCACTTTGAAT+CGG - chr5.3:17564537-17564556 MS.gene69614:CDS 40.0%
CGATTCAAAGTGCGATTTGT+GGG + chr5.3:17564539-17564558 None:intergenic 40.0%
CTGCACAAGTATATTGAGAG+CGG - chr5.3:17563942-17563961 MS.gene69614:intron 40.0%
CTTCGCTATATTCGTGTTTC+CGG + chr5.3:17564174-17564193 None:intergenic 40.0%
CTTTCGGTAAATATCTCACG+AGG + chr5.3:17567008-17567027 None:intergenic 40.0%
GATATTTACCGAAAGCCACA+AGG - chr5.3:17567013-17567032 MS.gene69614:intron 40.0%
GCCGATGATAATTATCGCAA+TGG - chr5.3:17564347-17564366 MS.gene69614:intron 40.0%
GCTTTGTATTCACACCCTTA+GGG + chr5.3:17565747-17565766 None:intergenic 40.0%
GGTGGAAACAATCAAGACAA+TGG - chr5.3:17567143-17567162 MS.gene69614:intron 40.0%
GTCTGGATTACAAGACGTAT+CGG + chr5.3:17565725-17565744 None:intergenic 40.0%
GTGAAGAAATTGGTGAAGGA+AGG - chr5.3:17564080-17564099 MS.gene69614:intron 40.0%
GTGACCTCTATTCCTGAAAT+TGG - chr5.3:17563431-17563450 MS.gene69614:CDS 40.0%
TAGAACGATAACAGGAACAC+TGG + chr5.3:17567444-17567463 None:intergenic 40.0%
TATAGCGCGGTTCAAATTCA+TGG + chr5.3:17566902-17566921 None:intergenic 40.0%
TATTTCAAGAGCTACACGGT+AGG + chr5.3:17566063-17566082 None:intergenic 40.0%
TCGGGGACATCCAATAAAAT+TGG + chr5.3:17565191-17565210 None:intergenic 40.0%
TCTGATCTTGATCGTAGATC+CGG - chr5.3:17566404-17566423 MS.gene69614:intron 40.0%
TCTGGATTACAAGACGTATC+GGG + chr5.3:17565724-17565743 None:intergenic 40.0%
TCTTGTAATCCAGACCCTAA+GGG - chr5.3:17565730-17565749 MS.gene69614:intron 40.0%
TGCACTTGGCAGGATATTTA+GGG - chr5.3:17566375-17566394 MS.gene69614:intron 40.0%
TGTCTTGATTGTTTCCACCA+GGG + chr5.3:17567142-17567161 None:intergenic 40.0%
TGTGCAACCATTATACGCAT+TGG - chr5.3:17564564-17564583 MS.gene69614:CDS 40.0%
TGTTACAAGCAGCAAAGCAT+GGG - chr5.3:17566156-17566175 MS.gene69614:intron 40.0%
TTACTCCTCAATTGTCCCTT+CGG + chr5.3:17565210-17565229 None:intergenic 40.0%
TTATCGCCAGTAAAGCAGTT+GGG - chr5.3:17565590-17565609 MS.gene69614:intron 40.0%
TTGCACTTGGCAGGATATTT+AGG - chr5.3:17566374-17566393 MS.gene69614:intron 40.0%
TTGTCTTGATTGTTTCCACC+AGG + chr5.3:17567143-17567162 None:intergenic 40.0%
TTTATCGCCAGTAAAGCAGT+TGG - chr5.3:17565589-17565608 MS.gene69614:intron 40.0%
! AAATTTTGTGTAGGCAGTGG+AGG - chr5.3:17566932-17566951 MS.gene69614:intron 40.0%
! AGCTGGTTCATTTACTCTTG+TGG - chr5.3:17567073-17567092 MS.gene69614:intron 40.0%
! ATTTTATTGGATGTCCCCGA+AGG - chr5.3:17565191-17565210 MS.gene69614:intron 40.0%
! CCAGTTTTCGTAGTTGTCTT+TGG + chr5.3:17563363-17563382 None:intergenic 40.0%
! CTGCAGAAATATTTGGTTCG+TGG - chr5.3:17564396-17564415 MS.gene69614:CDS 40.0%
! CTTTAGGAAGGTTATGTGGA+CGG - chr5.3:17565558-17565577 MS.gene69614:intron 40.0%
! GAGATTTGGAACAGGAAGAA+TGG - chr5.3:17563476-17563495 MS.gene69614:CDS 40.0%
! GTGAAATTTTGTGTAGGCAG+TGG - chr5.3:17566929-17566948 MS.gene69614:intron 40.0%
! GTTCGAAAACGGCTTTTCTA+CGG + chr5.3:17566280-17566299 None:intergenic 40.0%
! TCCTGATGGATACAATTGGA+GGG + chr5.3:17566012-17566031 None:intergenic 40.0%
! TTCCTGATGGATACAATTGG+AGG + chr5.3:17566013-17566032 None:intergenic 40.0%
! TTTTATTGGATGTCCCCGAA+GGG - chr5.3:17565192-17565211 MS.gene69614:intron 40.0%
!! AAGCGGAAGTAGATTAGTCT+TGG + chr5.3:17565316-17565335 None:intergenic 40.0%
!! CATGCTTTAATTTGCAGGTG+TGG - chr5.3:17563890-17563909 MS.gene69614:intron 40.0%
!! TTCCAAGTGGTAGTGGATAT+GGG - chr5.3:17564605-17564624 MS.gene69614:CDS 40.0%
!! TTTCCAAGTGGTAGTGGATA+TGG - chr5.3:17564604-17564623 MS.gene69614:CDS 40.0%
AAATGCCGTGTAGACGCATT+TGG - chr5.3:17566341-17566360 MS.gene69614:intron 45.0%
AACCCATATCCACTACCACT+TGG + chr5.3:17564610-17564629 None:intergenic 45.0%
AACTACCGCAACGTGAAGAA+CGG + chr5.3:17564042-17564061 None:intergenic 45.0%
AATTCCATGTTGCACTTGGC+AGG - chr5.3:17566365-17566384 MS.gene69614:intron 45.0%
AATTGTGCACCCCAAGTGTA+AGG - chr5.3:17566956-17566975 MS.gene69614:intron 45.0%
ACTTCGGTCCTTCTCTTCAT+TGG - chr5.3:17567242-17567261 MS.gene69614:CDS 45.0%
AGGGTAATCCTGACCTCTTA+TGG - chr5.3:17566207-17566226 MS.gene69614:CDS 45.0%
AGTATATTGAGAGCGGTGAG+TGG - chr5.3:17563949-17563968 MS.gene69614:intron 45.0%
ATATCCTGCCAAGTGCAACA+TGG + chr5.3:17566372-17566391 None:intergenic 45.0%
ATGCCTTCTGTGTTTCCAAG+TGG - chr5.3:17564592-17564611 MS.gene69614:CDS 45.0%
ATGCGTGCCAATGCGTATAA+TGG + chr5.3:17564574-17564593 None:intergenic 45.0%
CATAGCAAGTGAAGCACAGA+AGG + chr5.3:17567375-17567394 None:intergenic 45.0%
CATGTATGTATGCGAGTGAG+TGG + chr5.3:17566498-17566517 None:intergenic 45.0%
CCGATTCAAAGTGCGATTTG+TGG + chr5.3:17564540-17564559 None:intergenic 45.0%
CGCACCAATTTCAGGAATAG+AGG + chr5.3:17563438-17563457 None:intergenic 45.0%
CTACCACTTGGAAACACAGA+AGG + chr5.3:17564598-17564617 None:intergenic 45.0%
CTACGATCAAGATCAGAGGA+AGG + chr5.3:17566401-17566420 None:intergenic 45.0%
GACGGAACTAGAACGATAAC+AGG + chr5.3:17567452-17567471 None:intergenic 45.0%
GGATCTACGATCAAGATCAG+AGG + chr5.3:17566405-17566424 None:intergenic 45.0%
GGGTAATCCTGACCTCTTAT+GGG - chr5.3:17566208-17566227 MS.gene69614:CDS 45.0%
GTCTTGTAATCCAGACCCTA+AGG - chr5.3:17565729-17565748 MS.gene69614:intron 45.0%
GTGATGTCGTGCAAGAACAA+GGG + chr5.3:17567173-17567192 None:intergenic 45.0%
GTTCTTCTTGCTGCTGCTAA+GGG - chr5.3:17564272-17564291 MS.gene69614:intron 45.0%
TACTCCTCAATTGTCCCTTC+GGG + chr5.3:17565209-17565228 None:intergenic 45.0%
TAGCAGCAGCAAGAAGAACA+GGG + chr5.3:17564271-17564290 None:intergenic 45.0%
TCCCTCCAATTGTATCCATC+AGG - chr5.3:17566008-17566027 MS.gene69614:intron 45.0%
TCGTAGTTGTCTTTGGTCAG+TGG + chr5.3:17563356-17563375 None:intergenic 45.0%
TCTCTTTGCATCTGCATAGC+AGG + chr5.3:17566431-17566450 None:intergenic 45.0%
TCTGTGTTTCCAAGTGGTAG+TGG - chr5.3:17564598-17564617 MS.gene69614:CDS 45.0%
TCTTTACCGTTGTACACCGT+TGG - chr5.3:17564316-17564335 MS.gene69614:intron 45.0%
TGCAACTGTAAATGGACGGA+AGG - chr5.3:17566310-17566329 MS.gene69614:CDS 45.0%
TGCGATAATTATCATCGGCC+AGG + chr5.3:17564346-17564365 None:intergenic 45.0%
TGGCAATTCCATGTTGCACT+TGG - chr5.3:17566361-17566380 MS.gene69614:intron 45.0%
TGTGATGTCGTGCAAGAACA+AGG + chr5.3:17567174-17567193 None:intergenic 45.0%
TGTTCTTCTTGCTGCTGCTA+AGG - chr5.3:17564271-17564290 MS.gene69614:intron 45.0%
TTAGAACGCGCACCAATTTC+AGG + chr5.3:17563446-17563465 None:intergenic 45.0%
TTAGCAGCAGCAAGAAGAAC+AGG + chr5.3:17564272-17564291 None:intergenic 45.0%
TTGGAACAGGAAGAATGGGA+AGG - chr5.3:17563481-17563500 MS.gene69614:CDS 45.0%
! CATTGCCCTTTTCTTCTCTG+TGG - chr5.3:17567334-17567353 MS.gene69614:CDS 45.0%
! GAGGCATTTGAGGAAATCGT+TGG + chr5.3:17564513-17564532 None:intergenic 45.0%
! GGCTTTGTATTCACACCCTT+AGG + chr5.3:17565748-17565767 None:intergenic 45.0%
!! GCTTTAATTTGCAGGTGTGG+TGG - chr5.3:17563893-17563912 MS.gene69614:intron 45.0%
AAGTGCGATTTGTGGGCAAG+AGG + chr5.3:17564532-17564551 None:intergenic 50.0%
ACTCCTCAATTGTCCCTTCG+GGG + chr5.3:17565208-17565227 None:intergenic 50.0%
ATCTTGTGGGACATGGTCTG+TGG - chr5.3:17563402-17563421 MS.gene69614:CDS 50.0%
ATTCCACATCCTCTATGGGG+AGG - chr5.3:17564015-17564034 MS.gene69614:intron 50.0%
CACAAGGAGCTAGTGAAAGC+TGG - chr5.3:17567029-17567048 MS.gene69614:intron 50.0%
CACTAGCTCCTTGTGGCTTT+CGG + chr5.3:17567024-17567043 None:intergenic 50.0%
CAGCTCAGCAACAAGAGCTA+GGG + chr5.3:17564150-17564169 None:intergenic 50.0%
CAGCTTTCACTAGCTCCTTG+TGG + chr5.3:17567031-17567050 None:intergenic 50.0%
GCCGATCCAGATATTGAGCA+AGG - chr5.3:17563620-17563639 MS.gene69614:CDS 50.0%
GGTCTTCCAACGGTGTACAA+CGG + chr5.3:17564325-17564344 None:intergenic 50.0%
GTATTCACACCCTTAGGGTC+TGG + chr5.3:17565742-17565761 None:intergenic 50.0%
TCAATGGCCCATAAGAGGTC+AGG + chr5.3:17566218-17566237 None:intergenic 50.0%
TCACAACAAGGGTTCGCACA+AGG + chr5.3:17565665-17565684 None:intergenic 50.0%
TCAGCTCAGCAACAAGAGCT+AGG + chr5.3:17564151-17564170 None:intergenic 50.0%
TCGGGTGCAAAGCACACAAA+CGG + chr5.3:17565706-17565725 None:intergenic 50.0%
TCTTGTGGGACATGGTCTGT+GGG - chr5.3:17563403-17563422 MS.gene69614:CDS 50.0%
TTCACGTTGCGGTAGTTGCT+GGG - chr5.3:17564045-17564064 MS.gene69614:intron 50.0%
TTGCATCTGCATAGCAGGAC+CGG + chr5.3:17566426-17566445 None:intergenic 50.0%
TTGTACACCGTTGGAAGACC+TGG - chr5.3:17564325-17564344 MS.gene69614:intron 50.0%
TTTCAGCGTAACGAGCCGTT+AGG + chr5.3:17567271-17567290 None:intergenic 50.0%
!! TTGTGGGCAAGAGGCATTTG+AGG + chr5.3:17564523-17564542 None:intergenic 50.0%
AAGAACGGTCCTCCCCATAG+AGG + chr5.3:17564027-17564046 None:intergenic 55.0%
CTTCACGTTGCGGTAGTTGC+TGG - chr5.3:17564044-17564063 MS.gene69614:intron 55.0%
CTTGTGGGACATGGTCTGTG+GGG - chr5.3:17563404-17563423 MS.gene69614:CDS 55.0%
GAGGACCGTTCTTCACGTTG+CGG - chr5.3:17564034-17564053 MS.gene69614:intron 55.0%
GCTCTTGTTGCTGAGCTGAC+CGG - chr5.3:17564152-17564171 MS.gene69614:intron 55.0%
TCATCGGCCAGGTCTTCCAA+CGG + chr5.3:17564335-17564354 None:intergenic 55.0%
TGTCCCCGAAGGGACAATTG+AGG - chr5.3:17565202-17565221 MS.gene69614:intron 55.0%
! AGGAAGAGGCGCAACTGTGA+CGG + chr5.3:17567470-17567489 None:intergenic 55.0%
! GGAACAAGCATTGCAGCGAG+TGG - chr5.3:17563317-17563336 MS.gene69614:CDS 55.0%
GCAGCTTTCACTGTCCCTGG+TGG - chr5.3:17567125-17567144 MS.gene69614:intron 60.0%
GCTGCAGCTTTCACTGTCCC+TGG - chr5.3:17567122-17567141 MS.gene69614:intron 60.0%
GGTCCTCCCCATAGAGGATG+TGG + chr5.3:17564021-17564040 None:intergenic 60.0%
TGCGGTAGTTGCTGGGCACG+AGG - chr5.3:17564052-17564071 MS.gene69614:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 17563311 17567523 17563311 ID=MS.gene69614
chr5.3 mRNA 17563311 17567523 17563311 ID=MS.gene69614.t1;Parent=MS.gene69614
chr5.3 exon 17567241 17567523 17567241 ID=MS.gene69614.t1.exon1;Parent=MS.gene69614.t1
chr5.3 CDS 17567241 17567523 17567241 ID=cds.MS.gene69614.t1;Parent=MS.gene69614.t1
chr5.3 exon 17566424 17566927 17566424 ID=MS.gene69614.t1.exon2;Parent=MS.gene69614.t1
chr5.3 CDS 17566424 17566927 17566424 ID=cds.MS.gene69614.t1;Parent=MS.gene69614.t1
chr5.3 exon 17566182 17566343 17566182 ID=MS.gene69614.t1.exon3;Parent=MS.gene69614.t1
chr5.3 CDS 17566182 17566343 17566182 ID=cds.MS.gene69614.t1;Parent=MS.gene69614.t1
chr5.3 exon 17565393 17565494 17565393 ID=MS.gene69614.t1.exon4;Parent=MS.gene69614.t1
chr5.3 CDS 17565393 17565494 17565393 ID=cds.MS.gene69614.t1;Parent=MS.gene69614.t1
chr5.3 exon 17565223 17565270 17565223 ID=MS.gene69614.t1.exon5;Parent=MS.gene69614.t1
chr5.3 CDS 17565223 17565270 17565223 ID=cds.MS.gene69614.t1;Parent=MS.gene69614.t1
chr5.3 exon 17564945 17565004 17564945 ID=MS.gene69614.t1.exon6;Parent=MS.gene69614.t1
chr5.3 CDS 17564945 17565004 17564945 ID=cds.MS.gene69614.t1;Parent=MS.gene69614.t1
chr5.3 exon 17564368 17564804 17564368 ID=MS.gene69614.t1.exon7;Parent=MS.gene69614.t1
chr5.3 CDS 17564368 17564804 17564368 ID=cds.MS.gene69614.t1;Parent=MS.gene69614.t1
chr5.3 exon 17563311 17563889 17563311 ID=MS.gene69614.t1.exon8;Parent=MS.gene69614.t1
chr5.3 CDS 17563311 17563889 17563311 ID=cds.MS.gene69614.t1;Parent=MS.gene69614.t1
Gene Sequence

>MS.gene69614

ATGACAGGAACAAGCATTGCAGCGAGTGGAATAGTTCTTGAACCACTGACCAAAGACAACTACGAAAACTGGAGCTGTCTTGTTAGAAACTATCTTGTGGGACATGGTCTGTGGGGTGTTGTGACCTCTATTCCTGAAATTGGTGCGCGTTCTAAGACTGAATATGAGATTTGGAACAGGAAGAATGGGAAGGCATTGCATATCATTCAACTAACATGTGGACCAGAGAATCTTACTCATATTAGAGATCTTCATATTGCTAAAGAAGCCTGGAATAAATTGGGTGCAACTTATAGCTCAGATTTACAAGCCGATCCAGATATTGAGCAAGGTATATATGTAAATTTATATATATGTACACATTAATCTATTCTTTGGGTAAATCACGTAATGATCCCTATACATATTAGACATAGTTTCTAAATGTATTGAAAGGACAAAAGTATTTTTAAGTGTCTTTTGTGAGTGGGACACTAAAATAGATCTTCACTCATTTTCTTAAATACTTATAATATTAGGGTCCAATTAGTAAGGAAAATAAATGGAAGAAATATGTATATGTTTGTAGCTTAAAACATACATGCTTTAATTTGCAGGTGTGGTGGATGATAGTCTCCTTCAATATAAATCGCTGCACAAGTATATTGAGAGCGGTGAGTGGAAAGATGCAAACTCGTTTATTAAGAGGGACAGTACAGCAATATATTCCACATCCTCTATGGGGAGGACCGTTCTTCACGTTGCGGTAGTTGCTGGGCACGAGGAAATTGTGAAGAAATTGGTGAAGGAAGGGAAAGATAAGTTAGTGAAAATGAAAGACAATCGAGGTTACACAGCCCTAGCTCTTGTTGCTGAGCTGACCGGAAACACGAATATAGCGAAGTGTATGACTATGGTATATAAGAAAATAACACGGTCAGAGGACCTGTTATCTATGAAGACCAATGATGGTGAAATCCCTGTTCTTCTTGCTGCTGCTAAGGGACACAAAGAAATGACACGTTATCTTTACCGTTGTACACCGTTGGAAGACCTGGCCGATGATAATTATCGCAATGGTGTTTTGCTTCTTACACGATGTATCACTGCAGAAATATTTGGTTCGTGGTTACTGAATTTTAATTAAATTGAGGGTATTTTTCATATTAGTGTTTGATGTCGATTCAATATTGTATCTTAGGTGTGGCTTTGAGTTTACTCCAACGATTTCCTCAAATGCCTCTTGCCCACAAATCGCACTTTGAATCGGATTGTGTGCAACCATTATACGCATTGGCACGCATGCCTTCTGTGTTTCCAAGTGGTAGTGGATATGGGTTCATACGACAATTCTTTAACGATAGTAAGTTTTTTTTTTATTTTTTTTATTTTTTTCTGTTTCCGTTTCGCTTACTTTTCAAATTGGGTCATCTTAAAATTCTTGGTAGTGAAATAGCTAGTTATGCAAACTTATAATGTTACACGGTGAAATGAATTTTTATACAAATCTAAAATCATTTATACGTTGTTGAGATACATAAAGATAAATATTGTTTAATAAATGATCAATATAACTTTATAAAAGAAATTTAAAGGTGATTTATAAAAGGGAAATGCTAACCGGTGTCTTATAAAATACAAACTTTTGATCCAACATAATTTAACATCTCCACTACACATATAGTATTCATTTTATCATCTTTACTTCCAATAATATATTAAAGGAGTTTTACTTTAATCAAAAGAGTTTTACATGATCTGTTTGGAAATTGGAAGCATTATATATTATGCTGAATATTTTAAAACTCGATCTTGTGTATCCAAAAACTCATCAAACTTCACTCTTGTGCGATACTTTTCTTCTCAAGACCAACTTTACAAAATTAAGGAGTATCGTTCCAATTTTATTGGATGTCCCCGAAGGGACAATTGAGGAGTAAATAATGAATGAGATTGGAATAAAAATCATCAAACAAGATAAATACTCAAATTTTGCATTCAAGTGTTTTCTTTCTGTCTGCATCCAAGACTAATCTACTTCCGCTTTTAGTTTTGAGATTACCTGAAAGAGAAGTGCATGAATTTTACGGGATAATTGTGAGTCTTGCAAATATTGCCAAAGAGGAAAAACAACACAAAGCTTCTTTCGTAGGTATGGAATATTACACTCCTAATTCTTCTTTCTAGAGCTTATTATGTTCATGCAAGCAAGTTTTTCTACAATATGCAAATGGCGATTCTGAAGATTATTAATCTTCTTGGTTGTTTCTTTAGGAAGGTTATGTGGACGGATGTTGAATTTATCGCCAGTAAAGCAGTTGGGTAAGTTTTTTATCTATCAAATTCTTTTTAATAATTTTTATATATCAAAGCCTTGTGCGAACCCTTGTTGTGATCTGTGATCTTTTTGTTTCCGTTTGTGTGCTTTGCACCCGATACGTCTTGTAATCCAGACCCTAAGGGTGTGAATACAAAGCCAGTCCCTGTTATATATGTGTGTTGATATGTCATTTTTCTTTTACACATTTTTTTTTTTTCAGGTCGACTACTCATACTTGTTCACATGTCATTTCAGAATTCGGTACTATTAAAGTTCTCTGGTATGTAATTGTTAATCCTTGTTTAAATAGGTATATTAGATTGATAATTAAGATTATCCTTATTGTAAGTACAATCATTCCTCCCCTCTTTGTGATTAATTAATTAAATTTTTTTAAATCCCTCCAATTGTATCCATCAGGAATAAGGAAAATATATAATCAGAAAATGACCTACCGTGTAGCTCTTGAAATATTGAGTTGCTTGCACCAAAGAATTCAAGAGTTTAAGGAATCAGAACTTAGAGAGGCTTCGGCTTACGATGCAATGTTACAAGCAGCAAAGCATGGGATTATTGAGTTTATAGATGCAATGAGAAAGGGTAATCCTGACCTCTTATGGGCCATTGATAAAAATAAAAGAGGCGTATTTTCACATGCAATTTTGAACCGTAGAAAAGCCGTTTTCGAACTCATACATGATGCAACTGTAAATGGACGGAAGGAGATAGTTAAATGCCGTGTAGACGCATTTGGCAATTCCATGTTGCACTTGGCAGGATATTTAGGGCCTTCCTCTGATCTTGATCGTAGATCCGGTCCTGCTATGCAGATGCAAAGAGAAATTCTATGGTTCAAGGTGATTTCTTATGTCTCGTCTTTTTTTACCACTCACTCGCATACATACATGCATGCAGCTTATATGTTGGAACAAAGAGGTTATTTGATGATTTTGAGAGATAATAGAAGAGAGAACTGAGAAGAGATTAGAGAGTTTAGGAGAAAATAGACTTGTATTGTATGTTGAAAAATTCTCTAAAACAAACACATGTGTGAGCTTGTCTATTTTTAGACTACATGACTTGAGATACAAGTTAGCTCCTATAACAACTTCACAACTAACTTCAAAAACTAAATTTGAAATTCTGGAAACATTTATAATTTATTATAGTCAACATCCAATTTTGAGATACATGACATTTAAATAAGTGGTTAATTACGTTCTACAAATGATTAATGATTAGCCACACATTATTATCTTATTCATTAAGTGTTCACTGAATGTAATTAACCATGAATTTGAACCGCGCTATAACTTATTGTGAAATTTTGTGTAGGCAGTGGAGGAAATTGTGCACCCCAAGTGTAAGGAAGCAAAAAATGCTGACGATAAGAAGCCTCGTGAGATATTTACCGAAAGCCACAAGGAGCTAGTGAAAGCTGGAGAAAAATGGGCAAAAGATACAGCTGGTTCATTTACTCTTGTGGCTACACTCATCACTACTATCATGTTTGCTGCAGCTTTCACTGTCCCTGGTGGAAACAATCAAGACAATGGAGTTCCCTTGTTCTTGCACGACATCACATTTGATGTGTTTATTATAGCAGATGCAGCATCTCTTTTCACTTCTTCAACTTCGGTCCTTCTCTTCATTGGAATCCTAACGGCTCGTTACGCTGAAAAAGATTTCCTTAAGTCATTGCCACTAAGGTTACTTTTCGCACTCATTGCCCTTTTCTTCTCTGTGGTTTCTATGATAGTTGCCTTCTGTGCTTCACTTGCTATGCTCTTAAAGGGACATCATAGAGTCATAATAACAGCAATGTCATTTGCCAGTGTTCCTGTTATCGTTCTAGTTCCGTCACAGTTGCGCCTCTTCCTTGAGATTTTCAAGTCTACAGTATTATCCAATTGA
Protein sequence

>MS.gene69614.t1

MTGTSIAASGIVLEPLTKDNYENWSCLVRNYLVGHGLWGVVTSIPEIGARSKTEYEIWNRKNGKALHIIQLTCGPENLTHIRDLHIAKEAWNKLGVVDDSLLQYKSLHKYIESGEWKDANSFIKRDSTAIYSTSSMGRTVLHVAVVAGHEEIVKKLVKEGKDKLVKMKDNRGYTALALVAELTGNTNIAKCMTMVYKKITRSEDLLSMKTNDGEIPVLLAAAKGHKEMTRYLYRCTPLEDLADDNYRNGVLLLTRCITAEIFGVALSLLQRFPQMPLAHKSHFESDCVQPLYALARMPSVFPSGSGYGFIRQFFNDILRLPEREVHEFYGIIVSLANIAKEEKQHKASFVGRLCGRMLNLSPVKQLGRLLILVHMSFQNSVLLKFSGIRKIYNQKMTYRVALEILSCLHQRIQEFKESELREASAYDAMLQAAKHGIIEFIDAMRKGNPDLLWAIDKNKRGVFSHAILNRRKAVFELIHDATVNGRKEIVKCRVDAFGNSMLHLAGYLGPSSDLDRRSGPAMQMQREILWFKAVEEIVHPKCKEAKNADDKKPREIFTESHKELVKAGEKWAKDTAGSFTLVATLITTIMFAAAFTVPGGNNQDNGVPLFLHDITFDVFIIADAASLFTSSTSVLLFIGILTARYAEKDFLKSLPLRLLFALIALFFSVVSMIVAFCASLAMLLKGHHRVIITAMSFASVPVIVLVPSQLRLFLEIFKSTVLSN