AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69642

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69642.t1 MTR_5g028930 90.313 351 33 1 445 795 273 622 0.0 650
MS.gene69642.t1 MTR_5g028930 90.217 276 27 0 93 368 1 276 1.05e-167 498
MS.gene69642.t1 MTR_5g028190 40.777 618 259 16 182 789 12 532 3.91e-130 399
MS.gene69642.t1 MTR_5g028450 57.143 301 127 2 493 793 1 299 3.00e-105 325
MS.gene69642.t1 MTR_5g028130 48.246 342 165 5 453 789 2 336 7.37e-95 300
MS.gene69642.t1 MTR_5g028900 82.635 167 17 2 596 762 3 157 2.26e-87 273
MS.gene69642.t1 MTR_5g028690 61.165 206 80 0 590 795 36 241 8.46e-81 259
MS.gene69642.t1 MTR_5g028830 53.659 287 111 8 313 594 19 288 5.80e-78 253
MS.gene69642.t1 MTR_5g028920 81.507 146 24 1 452 597 85 227 1.68e-76 248
MS.gene69642.t1 MTR_5g028510 45.714 315 145 8 73 368 18 325 9.88e-71 236
MS.gene69642.t1 MTR_2g438740 36.570 309 181 5 484 788 269 566 1.42e-52 193
MS.gene69642.t1 MTR_5g028840 46.939 196 104 0 595 790 2 197 3.09e-50 175
MS.gene69642.t1 MTR_3g055310 39.004 241 140 3 530 770 94 327 9.92e-46 167
MS.gene69642.t1 MTR_3g055310 39.004 241 140 3 530 770 157 390 6.23e-45 167
MS.gene69642.t1 MTR_3g055310 39.004 241 140 3 530 770 209 442 1.69e-44 167
MS.gene69642.t1 MTR_5g028110 44.898 196 85 3 597 792 4 176 2.18e-44 158
MS.gene69642.t1 MTR_3g055310 39.004 241 140 3 530 770 237 470 2.77e-44 167
MS.gene69642.t1 MTR_3g055310 39.004 241 140 3 530 770 235 468 3.06e-44 167
MS.gene69642.t1 MTR_2g438760 36.066 244 155 1 496 739 273 515 4.13e-44 168
MS.gene69642.t1 MTR_6g017215 44.444 189 100 2 591 779 2 185 4.86e-44 159
MS.gene69642.t1 MTR_3g055310 39.004 241 140 3 530 770 262 495 7.33e-44 166
MS.gene69642.t1 MTR_5g091220 39.623 265 149 4 530 792 350 605 7.49e-44 168
MS.gene69642.t1 MTR_2g438720 33.559 295 187 4 496 788 404 691 1.27e-41 163
MS.gene69642.t1 MTR_2g438700 33.904 292 188 4 496 787 402 688 1.01e-40 160
MS.gene69642.t1 MTR_2g438400 33.106 293 190 4 496 788 258 544 4.85e-39 153
MS.gene69642.t1 MTR_2g438440 33.447 293 189 4 496 788 399 685 1.27e-38 154
MS.gene69642.t1 MTR_3g455790 37.500 224 137 2 526 749 341 561 1.40e-38 152
MS.gene69642.t1 MTR_2g438640 32.215 298 196 4 491 788 399 690 7.13e-37 148
MS.gene69642.t1 MTR_2g020510 33.452 281 175 4 493 770 390 661 3.37e-33 137
MS.gene69642.t1 MTR_3g452020 33.636 220 143 2 530 749 170 386 2.21e-32 131
MS.gene69642.t1 MTR_2g438640 31.544 298 172 4 491 788 399 664 3.68e-32 134
MS.gene69642.t1 MTR_3g452020 33.636 220 143 2 530 749 346 562 1.01e-31 131
MS.gene69642.t1 MTR_2g020450 34.520 281 171 6 493 770 393 663 3.97e-29 124
MS.gene69642.t1 MTR_2g020470 32.432 259 147 4 493 748 392 625 6.83e-28 120
MS.gene69642.t1 MTR_3g058570 30.392 306 198 5 489 787 80 377 1.44e-26 113
MS.gene69642.t1 MTR_2g017930 29.907 321 205 8 486 794 352 664 1.70e-25 112
MS.gene69642.t1 MTR_5g085340 28.763 299 185 6 493 777 443 727 1.09e-24 110
MS.gene69642.t1 MTR_5g085350 29.268 287 195 3 494 777 425 706 1.50e-24 110
MS.gene69642.t1 MTR_5g085350 29.167 288 196 3 493 777 437 719 1.73e-24 110
MS.gene69642.t1 MTR_3g050500 26.502 283 200 5 492 770 427 705 5.02e-24 108
MS.gene69642.t1 MTR_2g438640 34.536 194 122 3 595 788 399 587 3.28e-23 105
MS.gene69642.t1 MTR_5g085360 31.081 222 146 3 558 778 504 719 5.30e-23 105
MS.gene69642.t1 MTR_1g113820 30.415 217 148 3 491 707 274 487 2.04e-21 99.8
MS.gene69642.t1 MTR_3g051060 25.342 292 207 6 494 780 435 720 2.54e-21 99.8
MS.gene69642.t1 MTR_1g113760 30.131 229 149 4 484 707 231 453 4.60e-20 95.1
MS.gene69642.t1 MTR_1g113730 29.386 228 150 5 484 707 37 257 3.31e-19 90.5
MS.gene69642.t1 MTR_2g438470 32.090 134 89 2 538 671 366 497 4.89e-13 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69642.t1 AT3G18670 37.049 305 189 3 440 743 295 597 3.82e-56 204
MS.gene69642.t1 AT3G18670 37.329 292 180 3 453 743 253 542 6.69e-56 202
MS.gene69642.t1 AT5G04690 26.863 644 380 17 162 793 86 650 7.09e-50 187
MS.gene69642.t1 AT5G35810 34.028 288 179 2 468 750 16 297 3.85e-48 174
MS.gene69642.t1 AT5G04680 26.667 645 412 21 162 788 78 679 1.29e-47 181
MS.gene69642.t1 AT5G04690 26.128 643 372 17 162 793 86 636 1.42e-47 180
MS.gene69642.t1 AT5G04690 30.982 397 251 7 399 793 241 616 2.74e-47 179
MS.gene69642.t1 AT5G04730 31.909 351 228 5 447 793 250 593 1.88e-46 176
MS.gene69642.t1 AT5G04700 33.762 311 197 5 481 788 351 655 2.11e-46 177
MS.gene69642.t1 AT5G04680 31.348 319 202 6 481 788 215 527 3.48e-42 162
MS.gene69642.t1 AT5G04680 31.348 319 202 6 481 788 324 636 7.67e-42 163
MS.gene69642.t1 AT5G04680 31.348 319 202 6 481 788 338 650 7.96e-42 163
MS.gene69642.t1 AT3G54065 40.761 184 101 2 582 759 9 190 1.03e-34 132

Find 161 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAAATCCTTTAAGATTT+TGG 0.052410 5.3:+18042274 None:intergenic
AATCGTTTGTTTGGAAAATT+AGG 0.115432 5.3:+18043182 None:intergenic
CAAAATCTTAAAGGATTTAA+TGG 0.183831 5.3:-18042273 MS.gene69642:CDS
CTTAAAGGATTTAATGGTTT+AGG 0.191672 5.3:-18042267 MS.gene69642:CDS
TACTTGATCCAAAATCTTAA+AGG 0.197870 5.3:-18042282 MS.gene69642:CDS
TTAAAGGATTTAATGGTTTA+GGG 0.241629 5.3:-18042266 MS.gene69642:CDS
TGAATGTAATCGTTCCATTC+TGG 0.243091 5.3:+18042202 None:intergenic
TGCAGCAGCCTTTACTATTC+CGG 0.246841 5.3:-18040238 MS.gene69642:CDS
TGTATCGCGGCCGAAGTATT+TGG 0.249845 5.3:-18044453 MS.gene69642:intron
TGATTCTGCCGAGGCTTTCT+TGG 0.268497 5.3:-18048746 MS.gene69642:CDS
AAGAGAAATTCAATGGTTTA+AGG 0.273664 5.3:-18041929 MS.gene69642:intron
AGTCACTTGTCAGTGGTTTA+TGG 0.276993 5.3:-18046049 MS.gene69642:CDS
GACCGGCTTCAGAGACAATT+TGG 0.285322 5.3:+18046188 None:intergenic
CCTTATATACTCTAAAATTT+CGG 0.292125 5.3:+18042886 None:intergenic
ATACTCTAAAATTTCGGATT+CGG 0.307890 5.3:+18042892 None:intergenic
AAACAATTTGAAGACAACTA+TGG 0.308546 5.3:-18042920 MS.gene69642:CDS
TACTCTAAAATTTCGGATTC+GGG 0.309902 5.3:+18042893 None:intergenic
TGAAACATAAAGAGCTATTC+AGG 0.319238 5.3:-18044893 MS.gene69642:CDS
TTGTCAAAGGTGAGCAGTTC+AGG 0.319362 5.3:+18048838 None:intergenic
TTCAAGTATCTAGATATCTT+TGG 0.326074 5.3:-18042439 MS.gene69642:intron
GCAGCAGCCTTTACTATTCC+GGG 0.326327 5.3:-18040237 MS.gene69642:CDS
CAAGTGACTCCATGCATCTT+TGG 0.327706 5.3:+18046063 None:intergenic
ATTCTCCACCTTAGTTTCAT+TGG 0.348105 5.3:-18043222 MS.gene69642:CDS
GAATGTAATCGTTCCATTCT+GGG 0.353029 5.3:+18042203 None:intergenic
TCAAACATTTGCATACTTGT+TGG 0.357272 5.3:-18042470 MS.gene69642:CDS
ATCTATCCGCGGTGGCAGAT+GGG 0.360129 5.3:-18041971 MS.gene69642:CDS
GTGTATGGTAGATAGCAAAA+TGG 0.360420 5.3:-18044634 MS.gene69642:CDS
CGAACCTTGCTCAATATCTA+TGG 0.362493 5.3:+18048663 None:intergenic
CATCTATCCGCGGTGGCAGA+TGG 0.378276 5.3:-18041972 MS.gene69642:CDS
GTTTCTGCCTATAAAACTAC+TGG 0.384167 5.3:-18042777 MS.gene69642:CDS
CTTTCGATGAATACATCATA+AGG 0.384694 5.3:+18040354 None:intergenic
GGTTGGAGGAATCGTTTGTT+TGG 0.384788 5.3:+18043173 None:intergenic
TGTATGGTAGATAGCAAAAT+GGG 0.404241 5.3:-18044633 MS.gene69642:CDS
TCTCTTTGTACTTGCATAGC+AGG 0.405583 5.3:+18041946 None:intergenic
CGGAGAACTCCTCGTTCATA+TGG 0.406346 5.3:-18044580 MS.gene69642:CDS
AAGCTCATATATTCTCTCAA+TGG 0.408587 5.3:-18042075 MS.gene69642:CDS
CAAGGCCTTGAGAAAATCTA+TGG 0.416527 5.3:+18040071 None:intergenic
AACTAAGGTGGAGAATTTGT+TGG 0.419751 5.3:+18043229 None:intergenic
TTTCTCTGTCTGTTCCATGA+TGG 0.424250 5.3:-18040013 MS.gene69642:CDS
ATCGTTTGTTTGGAAAATTA+GGG 0.426153 5.3:+18043183 None:intergenic
GTCAGCACCTGGCCTCCTCA+TGG 0.433343 5.3:-18042813 MS.gene69642:CDS
GCAAAATCACATTGGCTCTA+GGG 0.433988 5.3:-18039956 MS.gene69642:CDS
GATGCTAGGAGAATGAAAAC+TGG 0.435157 5.3:+18039895 None:intergenic
TATTCCCACAACTTCATAGA+AGG 0.444082 5.3:+18042311 None:intergenic
TTGCCTGTGAAGTTAATAAC+TGG 0.445321 5.3:-18040051 MS.gene69642:CDS
AAATTTAGCCAATGAAACTA+AGG 0.448141 5.3:+18043214 None:intergenic
GTTGTTGATTTCACCGTATG+AGG 0.451688 5.3:+18043271 None:intergenic
CTAGGACCCATCTTGTCCCT+TGG 0.457620 5.3:-18039925 MS.gene69642:CDS
AGTTTGTAACAAAGCAAGAT+AGG 0.463408 5.3:-18044716 MS.gene69642:CDS
CAAAATCACATTGGCTCTAG+GGG 0.463524 5.3:-18039955 MS.gene69642:CDS
AACTGGTACACCCATCTCCT+TGG 0.464757 5.3:-18040199 MS.gene69642:CDS
CTCCTCGTTCATATGGCTGC+CGG 0.464883 5.3:-18044573 MS.gene69642:CDS
CATATATTCTCTCAATGGAT+TGG 0.465123 5.3:-18042070 MS.gene69642:CDS
AGCAAAATCACATTGGCTCT+AGG 0.465537 5.3:-18039957 MS.gene69642:CDS
GAATGAAACCTCTCTTTGAC+CGG 0.469232 5.3:+18046171 None:intergenic
ATATGCTAGATGTGGCTTTG+AGG 0.472243 5.3:-18043331 MS.gene69642:intron
CTTTCATTGTGTCAGGAACA+AGG 0.474209 5.3:-18042356 MS.gene69642:intron
TGAAGATATCAAAATAGATC+AGG 0.475051 5.3:-18044847 MS.gene69642:CDS
GAAATCAATGCAGGCGGTGG+AGG 0.476649 5.3:-18040427 MS.gene69642:intron
GGTGGAAACAATCAACAAAC+TGG 0.477838 5.3:-18040216 MS.gene69642:CDS
GTTCATATGGCTGCCGGGAA+AGG 0.481164 5.3:-18044567 MS.gene69642:CDS
AACAATTTGAAGACAACTAT+GGG 0.483905 5.3:-18042919 MS.gene69642:CDS
TCCTCGTTCATATGGCTGCC+GGG 0.496462 5.3:-18044572 MS.gene69642:CDS
ATCTGATAGGGCAAGGTCTG+TGG 0.500093 5.3:-18048783 MS.gene69642:CDS
TAGTGAAAGCTGGAGAGAAA+TGG 0.500591 5.3:-18040320 MS.gene69642:CDS
TGGAACAGACAGAGAAAAGA+AGG 0.504114 5.3:+18040019 None:intergenic
TTCGAAGCCATGAGGAGGCC+AGG 0.505506 5.3:+18042806 None:intergenic
AAGTACAAAGAGAAATTCAA+TGG 0.506830 5.3:-18041936 MS.gene69642:CDS
GTCACTGATAACCGTGGTAG+AGG 0.507156 5.3:-18042141 MS.gene69642:CDS
AATAGATATAGTGAGGTGTA+TGG 0.509587 5.3:-18044649 MS.gene69642:CDS
GTGAAATGTTGGTCAGCACC+TGG 0.511234 5.3:-18042824 MS.gene69642:CDS
AACACTACCAAGGGACAAGA+TGG 0.514768 5.3:+18039918 None:intergenic
TATACCTTCTATGAAGTTGT+GGG 0.515321 5.3:-18042315 MS.gene69642:CDS
TTACAAATGAAGTATTCTGC+AGG 0.516603 5.3:+18043152 None:intergenic
ATATACCTTCTATGAAGTTG+TGG 0.517376 5.3:-18042316 MS.gene69642:CDS
AAAGAGCTATTCAGGATGGT+GGG 0.521984 5.3:-18044885 MS.gene69642:CDS
AGGCCAGTTATTAACTTCAC+AGG 0.525985 5.3:+18040048 None:intergenic
TAGTGTCCTAGCTGTAATAG+AGG 0.528133 5.3:+18044806 None:intergenic
CACGAGGAGCTAGTGAAAGC+TGG 0.531806 5.3:-18040330 MS.gene69642:CDS
CACCAAATTGTCTCTGAAGC+CGG 0.532186 5.3:-18046190 MS.gene69642:CDS
AAATGAAGTATTCTGCAGGT+TGG 0.532813 5.3:+18043156 None:intergenic
TTGAATTAGCCAAAGATGCA+TGG 0.540504 5.3:-18046072 MS.gene69642:CDS
TGGAAATGTTGGTGAGAGAA+GGG 0.540997 5.3:-18044746 MS.gene69642:CDS
ATACTTCGGCCGCGATACAT+CGG 0.542225 5.3:+18044457 None:intergenic
CCATCTCTTGTAGTTGTCAA+AGG 0.542745 5.3:+18048825 None:intergenic
GTTTCCACCCGGAATAGTAA+AGG 0.543688 5.3:+18040230 None:intergenic
TGAAGTATTCTGCAGGTTGG+AGG 0.544573 5.3:+18043159 None:intergenic
CTTCACGTTGCAGTAAATGC+TGG 0.545487 5.3:-18044783 MS.gene69642:CDS
CTGCCACCGCGGATAGATGA+AGG 0.545727 5.3:+18041976 None:intergenic
CAGAGAAAAGAAGGAGCGCA+AGG 0.547894 5.3:+18040028 None:intergenic
CGTAAAATGAAAGCCACCGT+TGG 0.550114 5.3:-18048868 None:intergenic
CTGATGACGCATAATACAAA+CGG 0.552597 5.3:-18044600 MS.gene69642:CDS
CTCAGATACGCTTCATCTCG+AGG 0.552778 5.3:-18039873 MS.gene69642:CDS
TCAGTGACTGAAAGTAGATA+AGG 0.553905 5.3:+18042156 None:intergenic
ATGTTGTATGCAGCCCAGAA+TGG 0.559331 5.3:-18042216 MS.gene69642:CDS
TTCTCTCAATGGATTGGAGA+AGG 0.559507 5.3:-18042064 MS.gene69642:CDS
CCTTTGACAACTACAAGAGA+TGG 0.560459 5.3:-18048825 MS.gene69642:CDS
GCAGATGATGATGATATAGT+TGG 0.561795 5.3:-18044918 MS.gene69642:intron
AGCGTAAAATGCAACCATCA+TGG 0.563306 5.3:+18039999 None:intergenic
ACCGTGGTAGAGGCATACTT+TGG 0.564540 5.3:-18042131 MS.gene69642:CDS
AGCTAGTTGTTATATAGCTG+TGG 0.565436 5.3:-18040286 MS.gene69642:CDS
AATTCGCTTACCAAATACTT+CGG 0.565567 5.3:+18044443 None:intergenic
CATATTATTCAACTATCATG+TGG 0.566039 5.3:-18046124 MS.gene69642:CDS
TAAAGAGCTATTCAGGATGG+TGG 0.567740 5.3:-18044886 MS.gene69642:CDS
TCTGATAGGGCAAGGTCTGT+GGG 0.569720 5.3:-18048782 MS.gene69642:CDS
TTACATTCATAAATGCTATG+AGG 0.571803 5.3:-18042188 MS.gene69642:CDS
TGGAAGAGAACTCTGAAGCA+TGG 0.573519 5.3:-18048726 MS.gene69642:CDS
CTCTGAAGCCGGTCAAAGAG+AGG 0.574586 5.3:-18046179 MS.gene69642:CDS
TCTATCCGCGGTGGCAGATG+GGG 0.577220 5.3:-18041970 MS.gene69642:CDS
CGACATCTTCTGTGAAATGT+TGG 0.579216 5.3:-18042835 MS.gene69642:CDS
CTATTCAGGATGGTGGGGAG+AGG 0.579910 5.3:-18044879 MS.gene69642:CDS
GTGGAAATGTTGGTGAGAGA+AGG 0.582358 5.3:-18044747 MS.gene69642:CDS
CTGCTGCAAACATGACAGTA+AGG 0.582538 5.3:+18040253 None:intergenic
GTTTGTTGATTGTTTCCACC+CGG 0.586220 5.3:+18040219 None:intergenic
TTTCATTGTGTCAGGAACAA+GGG 0.587003 5.3:-18042355 MS.gene69642:intron
AGTTATTAACTTCACAGGCA+AGG 0.599770 5.3:+18040053 None:intergenic
GTTCTCTTCCAAGAAAGCCT+CGG 0.602884 5.3:+18048738 None:intergenic
AATCTAGATAAACTTGAGCA+AGG 0.603793 5.3:-18046010 MS.gene69642:intron
GCATTATAATAAGCTGCAAG+AGG 0.606169 5.3:+18044675 None:intergenic
TTTAGCCAATGAAACTAAGG+TGG 0.609057 5.3:+18043217 None:intergenic
ATCTTAAAGCAAAATCACAT+TGG 0.613795 5.3:-18039964 MS.gene69642:CDS
CCTTGAGAAAATCTATGGCA+TGG 0.614844 5.3:+18040076 None:intergenic
AGTTGTGCATCCGATGTGCA+AGG 0.618993 5.3:-18040403 MS.gene69642:CDS
ACCTTGCTCAATATCTATGG+TGG 0.620016 5.3:+18048666 None:intergenic
TGCATGGAGTCACTTGTCAG+TGG 0.624711 5.3:-18046056 MS.gene69642:CDS
GCTTCTTGACCGATGTATCG+CGG 0.625225 5.3:-18044466 MS.gene69642:CDS
TGAAGCGTATCTGAGATGCT+AGG 0.626094 5.3:+18039881 None:intergenic
ATATAACCTCTATTACAGCT+AGG 0.627187 5.3:-18044812 MS.gene69642:CDS
TGAAAACTGGAACACTACCA+AGG 0.630318 5.3:+18039908 None:intergenic
GAAATACAAGACTGAAGTGG+CGG 0.633365 5.3:+18040122 None:intergenic
AGTGATGATAGATACCGCAT+TGG 0.633541 5.3:+18040146 None:intergenic
ACATAAAGAGCTATTCAGGA+TGG 0.634401 5.3:-18044889 MS.gene69642:CDS
ACACTACCAAGGGACAAGAT+GGG 0.634544 5.3:+18039919 None:intergenic
GAAAACTGGAACACTACCAA+GGG 0.638165 5.3:+18039909 None:intergenic
GAAATTTCGAAGCCATGAGG+AGG 0.640112 5.3:+18042801 None:intergenic
AATGAAATACAAGACTGAAG+TGG 0.641503 5.3:+18040119 None:intergenic
TTAGTTCCTTCATCTATCCG+CGG 0.642808 5.3:-18041982 MS.gene69642:CDS
GTATGGTAGATAGCAAAATG+GGG 0.646932 5.3:-18044632 MS.gene69642:CDS
GATTAAATATCGCACAGACT+CGG 0.647343 5.3:-18042037 MS.gene69642:CDS
AAAATCACATTGGCTCTAGG+GGG 0.647874 5.3:-18039954 MS.gene69642:CDS
TTCAGGAACTATTCCAACGG+TGG 0.651974 5.3:+18048855 None:intergenic
GTAACAAAGCAAGATAGGCA+TGG 0.653388 5.3:-18044711 MS.gene69642:CDS
ACCACCATAGATATTGAGCA+AGG 0.653978 5.3:-18048667 MS.gene69642:intron
TATGGTAGATAGCAAAATGG+GGG 0.656522 5.3:-18044631 MS.gene69642:CDS
AAGAGCTATTCAGGATGGTG+GGG 0.660999 5.3:-18044884 MS.gene69642:CDS
TGACAATAATCTGTTGCACA+TGG 0.661381 5.3:-18042013 MS.gene69642:CDS
TTTCTGCCTATAAAACTACT+GGG 0.663280 5.3:-18042776 MS.gene69642:intron
CAGTTCAGGAACTATTCCAA+CGG 0.663409 5.3:+18048852 None:intergenic
GGTCTGTGGGATGTTGTCAG+TGG 0.667652 5.3:-18048769 MS.gene69642:CDS
AAATCACATTGGCTCTAGGG+GGG 0.667788 5.3:-18039953 MS.gene69642:CDS
CAGCTTTCACTAGCTCCTCG+TGG 0.669009 5.3:+18040331 None:intergenic
GCAGCCTTTACTATTCCGGG+TGG 0.673934 5.3:-18040234 MS.gene69642:CDS
GTTCCTTCATCTATCCGCGG+TGG 0.684957 5.3:-18041979 MS.gene69642:CDS
AATGTTTCTCTTAGCCAATG+CGG 0.690279 5.3:-18040160 MS.gene69642:CDS
TCCCGGCAGCCATATGAACG+AGG 0.690546 5.3:+18044571 None:intergenic
TGTCAGTGGTGATTCTGCCG+AGG 0.691609 5.3:-18048755 MS.gene69642:CDS
ACAGAAATTTCGAAGCCATG+AGG 0.695564 5.3:+18042798 None:intergenic
CAGGACCCCATCTGCCACCG+CGG 0.696635 5.3:+18041965 None:intergenic
ACCAAAGTATGCCTCTACCA+CGG 0.697647 5.3:+18042130 None:intergenic
CTTTCAGTCACTGATAACCG+TGG 0.726294 5.3:-18042147 MS.gene69642:CDS
TGTATTCATCGAAAGCCACG+AGG 0.749553 5.3:-18040346 MS.gene69642:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.3 gene 18039834 18048884 18039834 ID=MS.gene69642
chr5.3 mRNA 18039834 18048884 18039834 ID=MS.gene69642.t1;Parent=MS.gene69642
chr5.3 exon 18048668 18048884 18048668 ID=MS.gene69642.t1.exon1;Parent=MS.gene69642.t1
chr5.3 CDS 18048668 18048884 18048668 ID=cds.MS.gene69642.t1;Parent=MS.gene69642.t1
chr5.3 exon 18046011 18046226 18046011 ID=MS.gene69642.t1.exon2;Parent=MS.gene69642.t1
chr5.3 CDS 18046011 18046226 18046011 ID=cds.MS.gene69642.t1;Parent=MS.gene69642.t1
chr5.3 exon 18044454 18044936 18044454 ID=MS.gene69642.t1.exon3;Parent=MS.gene69642.t1
chr5.3 CDS 18044454 18044936 18044454 ID=cds.MS.gene69642.t1;Parent=MS.gene69642.t1
chr5.3 exon 18043150 18043344 18043150 ID=MS.gene69642.t1.exon4;Parent=MS.gene69642.t1
chr5.3 CDS 18043150 18043344 18043150 ID=cds.MS.gene69642.t1;Parent=MS.gene69642.t1
chr5.3 exon 18042777 18042953 18042777 ID=MS.gene69642.t1.exon5;Parent=MS.gene69642.t1
chr5.3 CDS 18042777 18042953 18042777 ID=cds.MS.gene69642.t1;Parent=MS.gene69642.t1
chr5.3 exon 18042440 18042502 18042440 ID=MS.gene69642.t1.exon6;Parent=MS.gene69642.t1
chr5.3 CDS 18042440 18042502 18042440 ID=cds.MS.gene69642.t1;Parent=MS.gene69642.t1
chr5.3 exon 18041930 18042363 18041930 ID=MS.gene69642.t1.exon7;Parent=MS.gene69642.t1
chr5.3 CDS 18041930 18042363 18041930 ID=cds.MS.gene69642.t1;Parent=MS.gene69642.t1
chr5.3 exon 18039834 18040436 18039834 ID=MS.gene69642.t1.exon8;Parent=MS.gene69642.t1
chr5.3 CDS 18039834 18040436 18039834 ID=cds.MS.gene69642.t1;Parent=MS.gene69642.t1
Gene Sequence

>MS.gene69642

ATGAAAGCCACCGTTGGAATAGTTCCTGAACTGCTCACCTTTGACAACTACAAGAGATGGAGTGCTATTATGAAAAATTATCTGATAGGGCAAGGTCTGTGGGATGTTGTCAGTGGTGATTCTGCCGAGGCTTTCTTGGAAGAGAACTCTGAAGCATGGAAGCATTTGAGCAACAAAAAAGGCAAAAAGATAGAAACCACCATAGATATTGAGCAAGGTTCGTATCTAATCTAATCTAATCTAAAAGTGATAAACCCTTACCCCATAATTATGTGTCTCCTTTATTTTATTTTTTTGACGGAAGTATCCTTAATTTATACTAAACTGAACTCCTCAATCTAAGCAAATAAAGAAAAAGAAAAAGAAAGTGAAGACCAATTTTATTTTTTGAAAAAAATTGTGAAACTGAAACCTAATTTGATTTTTGACAAAAATGAAAACAACTTAGCCCTACATGAACCACTGTTATAAAAAGGACAATCTAACCCCTCTATCACCTAAATAGAAACATATTTCTCATCACTGATTCATAATCAAGCATTCCCTTTTTAGGAGTGGAGAAAAGAGGGTTTTGGACAGCAGAAAATATATAAAAAAAATGGATAAATCTTTCTCATGTTTTGAAAGAGAAATTTTTTAGAGAATGATAAACTAATGTTTAAGATTAATATATTTTGAATTTAAAGAATATTATAATAATATAGATTGAATTTGAAGAATTCATACAAATCATCCAAGACCCTCCAAAACACTCCCCCATACAATTTTTGAGTTCCCCCAATATATTATGTATTATGAATAAAAAATTAACCTCCAAAGCTCTCTTCTCTACTCCCCCATACAATTTTTGAGTTCCCCCAATATATTTTGTATTATGAATAAAAAATTAACCTCCAAAGCCCTCTCCTTTCAATGTCTTCCATTTCTTCCACTTGTTTCTTTTTTTCTTTCTAAAACCCTCTCATCCCCCTCCCCTCCAAACTCCCAAACAAAGCTTAGAGGGGTTGGCTAGGGCAGTTAGAAAGGTCCCCTGTTTTGTTATCGTTGTGATTGCAATTTGGATTTGCTGTGAAGTGCTACACCATAACATCTGGTGTTGTGCACCATGCAACAATTTATACCACTCATGTATAGAGACGTCACGATCAAATCGGAGGCCCTGCTATAATATTAAAAATGAGGCCCTAATAAAGAGAACCACAATTTTTTTAATTCAAATTCAAGTATGTTTTTTTATTACGCTAACACTTGTAAATCTTCTATACTACTCCCTCTGTTTATTTTTCTTTTGATGTTTTAGGACTTCAACTATATTCCAAAACATTTGATATTTTAGTATATTTAATATTCTTTTATCAATTTTACTATTGTTGGGAAACACACCATAAAACTATTAAAGTAGTTGTAACCTTTGGCTTATCACATGAGAATTTAATTATAAATAAGGACATTTTAGTCCATAACATACAAATATTTATATACTTTAATTACTCCTAAAGACTTGTGTCAAGAGGTAAAACATCAAAAGATAAAAAACGGAGGGAGTATATATAAAGAAAATAGGTTTAAAATACACGAGTTTTGGCTGACATTTTTATCACCTATTTTACCCTTCATTTAAACTATTTTATATTCTATTTTATTCTTTAAAAATATTGTTTTAAATTTTAATTTAAAAAATTAAATCCCATTAAAATAGGAAATATCCCACTACAATGAAAACAATATTATCAATATATACGATGGGGGTATATATTTTGGTCTAAATTTTTTATCATTTCATTTATACCCTTAAACGAACTTTCCTATATCAATGATATTGTTGGTCGAGTTCAAAATCTCACTCCCACCCCATTTAGTGGCGGACTGACGAGTTGACGGGCCAGCCTGTCAAACAAAAACTATTTTTAATGTATTTTTTTACAACTTAATTGTTACAAAAAAATTATATTGTTTTTATCATTATAAAAAAATATATAAGAATAGGCATCAAATAAATTTGTGGTAAAAGATAGTTAACAAATTTTCGAATTCAACATCATAATTAATAGCAAAATAAGGCAAATCCACCGATATAAAACAAATGTGCAACATAAAACAAACCCAAAAAGTAATCGAAGAATAAATAACTGATATCCATTATAATTAACTGCCTACAATAACTTTGATGAAAACACAATTAGTAATGAATTTAATCAATAAAACAATCCGATTGGTAAAAAAAAATCAATAAAACAATCCGCAAGGAATATTTATGTACATGAAGAGATACATTGTAATTTGCCGGTTATGGATGAGGCAAAGTATAAGCAAGTATTGTATCAAATGCTACTATGTCTTTGTCCTATACACTTAAGCAACTCAAATAAAAATTGTTGATTGCATACTTGAACTAGCACACACAAAAAATTGAAAAACGGGTTAAGCGGGTCGGTCTGCCCCGTTTTTCCAATGAGTTAAGGGGGAGAACTAACTATAGAAAGCGGGTTCAAAATTTCAATTTAACCCACCAACAATAGCGGGCTAAACGGGCCGGTCCTCCGGGTTAGACCCATTTAACCACCCCTACTCTTTAGAAATAATCATGTCTACACACACTCTTTTTCCTCTAGTATCTTTGATTACAATGTAACGATCGATATGGTAAATATTTTAAAGGAATTGTCGACATTCACCAAATTGTCTCTGAAGCCGGTCAAAGAGAGGTTTCATTCAAGATGAAGAATGCAAAAGCTTTGCATATTATTCAACTATCATGTGGACGACAGATTCAAGATGAGCTTTGTCATTTTGAATTAGCCAAAGATGCATGGAGTCACTTGTCAGTGGTTTATGGCAAAACTTTAAAAATCAATCTAGATAAACTTGAGCAAGGTATGTATTGAAATGTAATTGCTACTTCCTCCTATCCTGTTTATACGAAATAATTAATTTTTTAAATCATTGTTTAATTGTTGTATCTGGTCTAAAATATAGACCAAGAGTAATGCTACGGACAGATGCTGTGACAGTCGCTTTTGACCACTTTCCTTCCTATTGGTCCACATCATTGTTATTAAAACATCTATTAACCGGCAACCGGCTATGTTTTTTATATTAAAAATTCTATATTTTCTAATTAACTTTGTCTCCCCACGCCTCAAATCACCATGTCACAACGACAACCATCTTCATGGCCAAAGCTTGACGTAGTTGTGTGAAAGACAATTGGATCTTTGAACTGTGAGATTAGATGAATCTTTGAAAAATGTTTGAATAGCATTCCATCAGATTGGGTGTGAGAAGCTCAATTTTGCAGGCAATCTCTCTTTGACAAAACACCACAACTCAGTCCTTAAGTCACTGAAAATTCAGATCCATTCAAACCCAGATCTGACAATTATTTGCATGAGAAAATTAATTTAATGTAGATTTAAAGTTTATTGGAATAATTAGACTTGGCCCTTTATGAAATTTGGGAAAAGATTTGGTTTTTGGTTGATGTTTATTTGATTAAGGGGCTGAAACTTGAAGGTTGGTTATCGGGTTGTTGTGATATCGGTCAAAATCCTTATTTCTGCCAAATATTTGCTATGAGCATATGGGTTGTTGTGATAGCCATGGGTGTTGCGACATGGGTGCTGTGAGCATGTCTTTTCCAGATCTTCTTCCTTCTTGCAAAAAAAATAAATAAAAAAAATAAAATAAAACTAAACTTGAGGAGCCTGTAAACAAGTAAATGAGTTTAAAGAGAAGTGTGACGTGGCCCAATCCGTGAGAGAGTGTCCGGCCGAGAGTGTACAAGCGACTGATGCTAGCATGGCTCTATAGACCAAATACATCAACTATTTAATAAATCTAAAAAGTTGATTGTTTCTTATAAATAGTAAAGGGAACATATAAATACTGCGAGTTGTATTGATTTTCCCTCCAAGATAATGTTTTTGCATGTTTAAACGTAAATGGCAGATGATGATGATATAGTTGGTGTGAAACATAAAGAGCTATTCAGGATGGTGGGGAGAGGTGATCCTATTGAAGATATCAAAATAGATCAGGATGTATATGATGATATAACCTCTATTACAGCTAGGACACTACTTCACGTTGCAGTAAATGCTGGAAATCTGAAAAATGTGGAAATGTTGGTGAGAGAAGGGAGAGATGAGTTTGTAACAAAGCAAGATAGGCATGGTGACACAGCTCTTCCTCTTGCAGCTTATTATAATGCAAAAATAGATATAGTGAGGTGTATGGTAGATAGCAAAATGGGGGAGATGTTGCTGATGACGCATAATACAAACGGAGAACTCCTCGTTCATATGGCTGCCGGGAAAGGCCACAAAAAAATGACTAGTTTTCTTTACTCAAAAACTCCCGGAGAAGTGTTCAAAAAAGATTCAGGATATCGAGTTTTGCTTCTTGACCGATGTATCGCGGCCGAAGTATTTGGTAAGCGAATTTAAAAGTTCCAATTGTGTTACAAGTTAAAGAACTATATAAAGAATTAAAAAAATAAAATTTGTATTGAAAAGATAAATTTCTTAACACTTTAAAAAAAACTTTTAAAACATTAATTCCATAATTTTCAAAAAAAATATTTCCATCTTTAGCCGTGCCTTAAGGACACATTCTAACATTTCTCTTAAATAATTTTAAGTGCACTTATTATTTTGTGTGTATAGGTGTGTACGTAGTGTGTGTTTCCTCCATTAATTTTTGTTAAATATTTTGTTTTAATTTTGAAAAAAATTGTTTCAACTAATTTATCTGTTTCCCCCCAAAATGTCAAGGTATTAACTACTCTCTCTTCCACCAATAAAATATTAACCACTCTCGATTCTTTTCTTTTTTATAAAAAAATTGCTCATTTTTATTTCGTGTTTGTAATGTTCAAAGTACTAGCGTCATAATAATTGGCACTTTTGTTAAAAACAATTGTAAAAAATATTTTTAATTTTAAACGTCATATTATTTTTTCTTCTTCTTTCAATTATACCTTCTATGTATGGTATGTGTCACCCTCAAGTTATGATCTTTTTTATTATTCATTTATGATAACAAGAATCAATCAATGACTAACAAAGTTAATATAGTAAAGTTGTTAGTATTATTTTTTTTTTTTTTGTACAAAGTTTTTAGTATTATATTTCCTTCATTTATATCTTCTCTTTAATTTGTAAAATAACCTAATATGTACATTTTTCTGGGACACAAAAAATATTAAAAAATAACCTCTTTAATTTGTAAAATAACCTAATATGTACATTTTTCTGGGACACAAAAAATATAAAAAAATTTCGGCAGAATTATCTATAACTGTTTGCAAAAGGAAAAATAGGTGTAATGAAATTAAAAGGATATGAAGTCATTTTAATAATAAATGAACTTTAAAATTTACAATTTTTTCTTATATTTATAAAAAAAAATGATTTGCAATTTTTTTTTCGTATAAGGGGCCAAAGAAAAGGTTGGGTAAAACAATCTTAAATAAACAAGCATTAGAAGAATATTAGATTGAATTTCATCTTGACTTTTCAAATGTGTGGTTGATATGCTAGATGTGGCTTTGAGGTTACTCAAACTCTACCCCAATGATCTTTTTCATGAAGCCTCATACGGTGAAATCAACAACGAAGAACGAAATGAAGAATCCAACAAATTCTCCACCTTAGTTTCATTGGCTAAATTTAAATTGCACCCTAATTTTCCAAACAAACGATTCCTCCAACCTGCAGAATACTTCATTTGTAAATGTAAGTTTTCAAGATTTGCCTTTTATGTACCAAGCGTCTAATATAAACTACTATATTTTTCTTCATTTTTTATTAATATAAAACTTTTTTTCACTAAGGGTTAACCTTTGATTATATTTTGAGAGGTTCATATTAGAAACACAATTTATGTCTATCACACAATAATTGATCTATTCCTAAAACAAATTTATTACAGATTTAAGCCTAAAACAATTTGAAGACAACTATGGGATTCCCGAATCCGAAATTTTAGAGTATATAAGGTCGGTTTATGCAGATAGAAGTGTACATGCGACATCTTCTGTGAAATGTTGGTCAGCACCTGGCCTCCTCATGGCTTCGAAATTTCTGTTTCTGCCTATAAAACTACTGGGTATGTTGATTCCATTCCCCTTACAAATCATCGAATTTACTATAACGGGGTAAATAGTGCTAATAAGAAGTAGTTCTCAAACACTCTATTTAGTACTTACTCTTTTATTAATTTAAATTATCGTGGCTTATACATTTTAAAAATAGAACATATACAAAATGGTGGGACATACATTTATGCTATCTAATAAAAAAGTGAATTGTAAAGATCGTTTTAAAAATGACTATTGCTAACATTTATTTATTTTTACCGGATGGTTTGTTGATTTGTTCAGATCGACTACTTCAAACATTTGCATACTTGTTGGTTGAAGTTTTCAAGTATCTAGATATCTTTGGTAAGCAATATTACTCTCATTTAATTTGATTTTCGGCAATGATTCATGTACTTATAAGTTCCTTTCATTGTGTCAGGAACAAGGGAAATATATGCAAGAAAATATACCTTCTATGAAGTTGTGGGAATATTGAAATACTTGATCCAAAATCTTAAAGGATTTAATGGTTTAGGGCTCAGACAAGCTTCAGCACATGAAGCTATGTTGTATGCAGCCCAGAATGGAACGATTACATTCATAAATGCTATGAGGAATGCTAATCCTTATCTACTTTCAGTCACTGATAACCGTGGTAGAGGCATACTTTGGTATGCAATTCTGAAACGCAGAAGATATGTTTTCAAGCTCATATATTCTCTCAATGGATTGGAGAAGGAGATGATTAAATATCGCACAGACTCGGTTGACAATAATCTGTTGCACATGGCAGCACTTTTAGTTCCTTCATCTATCCGCGGTGGCAGATGGGGTCCTGCTATGCAAGTACAAAGAGAAATTCAATGGTTTAAGGTCATTTCTCTCATCTCTATCTATGATCTGATCTATCTGTCTGTCTATCTATCTATCTATCTATAACGGGAACAAACTACAAAGCTGGTCGTGAGGTACAAACACCTCTGATGTCTTGAATTAAAAGTAGATCAAGCAAAAGAGATATTGAATTGACAGAACAATTAACAGGTTTAGAACAATACTCTTGTATGAAAGTGATTTGGTGAAGAAAGAAAGTAATGAGAACTTTGGAGATTAGGAGAGAATGGAGATGATAAGAAATCCAATTTTTTATTGAATGATCGATTAGGGACTATTTATGAAACATGGGAATCAACAACTAACTAAAACTAAGTTGTAACTTGTAGCTCAAGTTACCTAACTAATATTTAGTTATTCAACTAGCAACCTTACTAACTTTGAATTATATCAACTAAAATCCTCAAATCAAAGTTAGTTGTAAATACCATTACCAATCTTCTTCTAAGGTGTTCCAATGTGCTAGTCTTCGAAGGCTTTGTTAGCAAATCTGCAAGTTTTAAATCTGTTGCAATATTGCAGCTCCGATTTCTCCTTGCTTAATTGATCCCTTAGAAAATGAAACTTTGTTTCAATTTGTTTGCTCCGCCCATGAGCAACTTGGTGCTTTGCAAGATCAATTGATGACTTGTTATCTATTAGCAATATAAAGCCATCTTCTCAGCTTCCTTCAAGTTCATCTCTTCCATTAGAATGCTTAACATGCAACTATTGAAGTTGCAATGTACTCTGCTTCACAACAGGATAGAGTTATTACTATTTCCTTCTTAGAACACCGTGACACTGGTGCATTCTCATACATCACATATGCACATTCCTTTTCCTATCATTTAAACTAACAACCTAACTAACTTTAAATTATATCAACTAGTACAAATATATAGTAATCACATTCAAAGGGGTAGAGACACCCTTATTAGCTAGTAAATATGCACACTTGTTGACTTCATGGTAGACGTGGACAATAAAAGTTCTCCAGTTTGGTGGTTAGAGAAGAGAAATGATATCATCAAGAAGAGGTTGACGGAAGCGTATATGAGTCTTTTTAGAATTCACCATATCAACTACCACCTTAGAATCCATCTAAAAAATGATCTTAGAAAGTGATAATTCTTGAGCAACCACCACCTTAGAATCCATCTAAAAAAATGACATAAGAACGTGATAATTATTGGACTATTACTTTTCCTATCTTATGAGTTTCTCGCGCGCCCTATTAGACACATGAGATACAAGTGGCAATAACACACGACAATTAAATGGTGGGAAGTACACATACATACACTATCTGTTGGAACAATATTTAAAATTAGGAGTGGTCCAATGGGCGGAGAGATTGTAGAGGTTTTCAAGAAACATCATTATTTAAGATATTAGATTGCTTTATTGACACTTGCCAAAAATATTTTTTACAAGTGTTTTCAAATGTATAATATTGAGATTAAGATTAATATTGTTTGAAATCAATGCAGGCGGTGGAGGAAGTTGTGCATCCGATGTGCAAGGAAGCCAAAAATGAGGATGGTAAGAAACCTTATGATGTATTCATCGAAAGCCACGAGGAGCTAGTGAAAGCTGGAGAGAAATGGACAAAAGACACAGCTAGTTGTTATATAGCTGTGGCTTCTCTCGTCCTTACTGTCATGTTTGCAGCAGCCTTTACTATTCCGGGTGGAAACAATCAACAAACTGGTACACCCATCTCCTTGGATCAAAATACATTTAAAATGTTTCTCTTAGCCAATGCGGTATCTATCATCACTTCCGCCACTTCAGTCTTGTATTTCATTTCGATTCTCACATCGAGTTGCCATGCCATAGATTTTCTCAAGGCCTTGCCTGTGAAGTTAATAACTGGCCTTGCGCTCCTTCTTTTCTCTGTCTGTTCCATGATGGTTGCATTTTACGCTGCGCTTAATATGATCTTAAAGCAAAATCACATTGGCTCTAGGGGGGTTGTTCTAGGACCCATCTTGTCCCTTGGTAGTGTTCCAGTTTTCATTCTCCTAGCATCTCAGATACGCTTCATCTCGAGGATTTTATATTCTACAATGAAAAATCGCATTAAGGGATGA
Protein sequence

>MS.gene69642.t1

MKATVGIVPELLTFDNYKRWSAIMKNYLIGQGLWDVVSGDSAEAFLEENSEAWKHLSNKKGKKIETTIDIEQGIVDIHQIVSEAGQREVSFKMKNAKALHIIQLSCGRQIQDELCHFELAKDAWSHLSVVYGKTLKINLDKLEQDDDDIVGVKHKELFRMVGRGDPIEDIKIDQDVYDDITSITARTLLHVAVNAGNLKNVEMLVREGRDEFVTKQDRHGDTALPLAAYYNAKIDIVRCMVDSKMGEMLLMTHNTNGELLVHMAAGKGHKKMTSFLYSKTPGEVFKKDSGYRVLLLDRCIAAEVFDVALRLLKLYPNDLFHEASYGEINNEERNEESNKFSTLVSLAKFKLHPNFPNKRFLQPAEYFICKYLSLKQFEDNYGIPESEILEYIRSVYADRSVHATSSVKCWSAPGLLMASKFLFLPIKLLDRLLQTFAYLLVEVFKYLDIFGTREIYARKYTFYEVVGILKYLIQNLKGFNGLGLRQASAHEAMLYAAQNGTITFINAMRNANPYLLSVTDNRGRGILWYAILKRRRYVFKLIYSLNGLEKEMIKYRTDSVDNNLLHMAALLVPSSIRGGRWGPAMQVQREIQWFKAVEEVVHPMCKEAKNEDGKKPYDVFIESHEELVKAGEKWTKDTASCYIAVASLVLTVMFAAAFTIPGGNNQQTGTPISLDQNTFKMFLLANAVSIITSATSVLYFISILTSSCHAIDFLKALPVKLITGLALLLFSVCSMMVAFYAALNMILKQNHIGSRGVVLGPILSLGSVPVFILLASQIRFISRILYSTMKNRIKG