Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69740.t1 | GAU47270.1 | 77.7 | 103 | 23 | 0 | 1 | 103 | 150 | 252 | 9.30E-35 | 156.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69740.t1 | A0A2Z6PBJ3 | 77.7 | 103 | 23 | 0 | 1 | 103 | 150 | 252 | 6.7e-35 | 156.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052333 | MS.gene69740 | 0.807236 | 5.58E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene69740 | 0.802377 | 5.78E-49 | -1.69E-46 |
| MS.gene055227 | MS.gene69740 | 0.814304 | 1.65E-51 | -1.69E-46 |
| MS.gene055228 | MS.gene69740 | 0.807856 | 4.12E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene69740 | 0.801412 | 9.11E-49 | -1.69E-46 |
| MS.gene056252 | MS.gene69740 | 0.814413 | 1.56E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene69740 | 0.807295 | 5.42E-50 | -1.69E-46 |
| MS.gene060011 | MS.gene69740 | 0.840221 | 9.91E-58 | -1.69E-46 |
| MS.gene060322 | MS.gene69740 | 0.807004 | 6.25E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69740.t1 | MTR_1g021965 | 60.377 | 53 | 21 | 0 | 18 | 70 | 112 | 164 | 2.55e-16 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCTCGACTCCAAAGCTT+TGG | 0.344968 | 3.4:-77024093 | None:intergenic |
| GTTGTTTGCGTGTATTGTTT+TGG | 0.356104 | 3.4:-77024124 | None:intergenic |
| TGCTCTCGAGTGCAAAGCTT+TGG | 0.369375 | 3.4:+77024487 | MS.gene69740:CDS |
| AGCTGCACGAGCCAAAGCTT+TGG | 0.379774 | 3.4:+77024082 | MS.gene69740:CDS |
| GCGGAGAACATCATACTTCC+AGG | 0.389492 | 3.4:+77023912 | MS.gene69740:CDS |
| TCTCCGCCTTCAATTCTTCA+AGG | 0.400192 | 3.4:-77023896 | None:intergenic |
| AGCTCTGCATTCCATTCTGC+TGG | 0.426420 | 3.4:+77024517 | MS.gene69740:CDS |
| CGTGTTTGTCGCTGATGCCT+TGG | 0.446247 | 3.4:+77023935 | MS.gene69740:CDS |
| CCTTCAATTCTTCAAGGAGT+TGG | 0.448637 | 3.4:-77023890 | None:intergenic |
| CCAACTCCTTGAAGAATTGA+AGG | 0.465521 | 3.4:+77023890 | MS.gene69740:CDS |
| TTGTAATCATTCCATGAGTC+GGG | 0.479069 | 3.4:-77023969 | None:intergenic |
| TTGAAAAGTTGCCCGACTCA+TGG | 0.500599 | 3.4:+77023958 | MS.gene69740:CDS |
| CATTGAAGACACCAGCAGAA+TGG | 0.535892 | 3.4:-77024528 | None:intergenic |
| CTTGTAATCATTCCATGAGT+CGG | 0.537899 | 3.4:-77023970 | None:intergenic |
| TCTGTAATGGCAAATGAAAG+AGG | 0.555816 | 3.4:+77023833 | None:intergenic |
| GCATCAGCGACAAACACGCC+TGG | 0.558250 | 3.4:-77023930 | None:intergenic |
| ATGGCAAATGAAAGAGGACA+AGG | 0.655713 | 3.4:+77023839 | None:intergenic |
| ACAATACACGCAAACAACAG+AGG | 0.686335 | 3.4:+77024129 | MS.gene69740:CDS |
| GACACCAGCAGAAAAGAATG+CGG | 0.696693 | 3.4:+77024059 | MS.gene69740:CDS |
| AGGACAAGGAAATCAAAGTG+CGG | 0.706319 | 3.4:+77023853 | MS.gene69740:CDS |
| ACTCCTTGAAGAATTGAAGG+CGG | 0.711654 | 3.4:+77023893 | MS.gene69740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATAGATATCGAAAAGTGAA+TGG | - | chr3.4:77024328-77024347 | None:intergenic | 25.0% |
| !! | ACCTAGGTTTGATTTAAAAT+TGG | + | chr3.4:77024388-77024407 | MS.gene69740:intron | 25.0% |
| !! | CCTAGGTTTGATTTAAAATT+GGG | + | chr3.4:77024389-77024408 | MS.gene69740:intron | 25.0% |
| !! | CTAGGTTTGATTTAAAATTG+GGG | + | chr3.4:77024390-77024409 | MS.gene69740:intron | 25.0% |
| ! | CCCAATTTTAAATCAAACCT+AGG | - | chr3.4:77024392-77024411 | None:intergenic | 30.0% |
| ! | CTAGGTTCGATTTAAAATTG+AGG | - | chr3.4:77024193-77024212 | None:intergenic | 30.0% |
| AAGCATATCACTGCAGATAT+TGG | + | chr3.4:77024246-77024265 | MS.gene69740:intron | 35.0% | |
| CTTGTAATCATTCCATGAGT+CGG | - | chr3.4:77023973-77023992 | None:intergenic | 35.0% | |
| GTTGTTGTGTGTGTAAAGAA+AGG | - | chr3.4:77024367-77024386 | None:intergenic | 35.0% | |
| TTGTAATCATTCCATGAGTC+GGG | - | chr3.4:77023972-77023991 | None:intergenic | 35.0% | |
| ! | AATTGAGGTTAGTTACTCTG+AGG | - | chr3.4:77024178-77024197 | None:intergenic | 35.0% |
| ! | ATTGAGGTTAGTTACTCTGA+GGG | - | chr3.4:77024177-77024196 | None:intergenic | 35.0% |
| ! | GTTGTTTGCGTGTATTGTTT+TGG | + | chr3.4:77024456-77024475 | MS.gene69740:intron | 35.0% |
| ! | GTTGTTTGCGTGTATTGTTT+TGG | - | chr3.4:77024475-77024456 | None:intergenic | 35.0% |
| !! | ACCTGAGTCAAATTTGCTTT+TGG | + | chr3.4:77024289-77024308 | MS.gene69740:intron | 35.0% |
| !!! | AAATTTGCTTTTGGCTTAGG+AGG | + | chr3.4:77024298-77024317 | MS.gene69740:intron | 35.0% |
| !!! | GTCAAATTTGCTTTTGGCTT+AGG | + | chr3.4:77024295-77024314 | MS.gene69740:intron | 35.0% |
| ACAATACACGCAAACAACAG+AGG | + | chr3.4:77024129-77024148 | MS.gene69740:CDS | 40.0% | |
| ACAATACACGCAAACAACAG+AGG | - | chr3.4:77024148-77024129 | None:intergenic | 40.0% | |
| ACTCCTTGAAGAATTGAAGG+CGG | + | chr3.4:77023893-77023912 | MS.gene69740:CDS | 40.0% | |
| AGGACAAGGAAATCAAAGTG+CGG | + | chr3.4:77023853-77023872 | MS.gene69740:CDS | 40.0% | |
| CACACACATAGCAACAATCT+AGG | - | chr3.4:77024211-77024230 | None:intergenic | 40.0% | |
| CCAACTCCTTGAAGAATTGA+AGG | + | chr3.4:77023890-77023909 | MS.gene69740:CDS | 40.0% | |
| CCTTCAATTCTTCAAGGAGT+TGG | - | chr3.4:77023893-77023912 | None:intergenic | 40.0% | |
| CTAGATTGTTGCTATGTGTG+TGG | + | chr3.4:77024209-77024228 | MS.gene69740:intron | 40.0% | |
| GCCAAAAGCAAATTTGACTC+AGG | - | chr3.4:77024293-77024312 | None:intergenic | 40.0% | |
| TACACACACAACAACAACCT+AGG | + | chr3.4:77024372-77024391 | MS.gene69740:intron | 40.0% | |
| TGCTATGTGTGTGGAAAGAA+AGG | + | chr3.4:77024218-77024237 | MS.gene69740:intron | 40.0% | |
| !! | AATTGGGGTTAGTTACTCTG+AGG | + | chr3.4:77024405-77024424 | MS.gene69740:intron | 40.0% |
| !! | ATTGGGGTTAGTTACTCTGA+GGG | + | chr3.4:77024406-77024425 | MS.gene69740:intron | 40.0% |
| CATTGAAGACACCAGCAGAA+TGG | - | chr3.4:77024071-77024052 | None:intergenic | 45.0% | |
| GACACCAGCAGAAAAGAATG+CGG | + | chr3.4:77024059-77024078 | MS.gene69740:CDS | 45.0% | |
| TCTCCGCCTTCAATTCTTCA+AGG | - | chr3.4:77023899-77023918 | None:intergenic | 45.0% | |
| TTCTCTCGACTCCAAAGCTT+TGG | - | chr3.4:77024096-77024115 | None:intergenic | 45.0% | |
| TTGAAAAGTTGCCCGACTCA+TGG | + | chr3.4:77023958-77023977 | MS.gene69740:CDS | 45.0% | |
| !!! | TTGCTTTTGGCTTAGGAGGT+TGG | + | chr3.4:77024302-77024321 | MS.gene69740:intron | 45.0% |
| AGCTCTGCATTCCATTCTGC+TGG | + | chr3.4:77024517-77024536 | MS.gene69740:CDS | 50.0% | |
| GCGGAGAACATCATACTTCC+AGG | + | chr3.4:77023912-77023931 | MS.gene69740:CDS | 50.0% | |
| TGCTCTCGAGTGCAAAGCTT+TGG | + | chr3.4:77024487-77024506 | MS.gene69740:CDS | 50.0% | |
| ! | AGCTCCGCATTCTTTTCTGC+TGG | - | chr3.4:77024066-77024085 | None:intergenic | 50.0% |
| ! | GTCGGGCAACTTTTCAACCA+AGG | - | chr3.4:77023955-77023974 | None:intergenic | 50.0% |
| AGCTGCACGAGCCAAAGCTT+TGG | + | chr3.4:77024082-77024101 | MS.gene69740:CDS | 55.0% | |
| CGTGTTTGTCGCTGATGCCT+TGG | + | chr3.4:77023935-77023954 | MS.gene69740:CDS | 55.0% | |
| GCATCAGCGACAAACACGCC+TGG | - | chr3.4:77023933-77023952 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 77023846 | 77024577 | 77023846 | ID=MS.gene69740 |
| chr3.4 | mRNA | 77023846 | 77024577 | 77023846 | ID=MS.gene69740.t1;Parent=MS.gene69740 |
| chr3.4 | exon | 77023846 | 77024150 | 77023846 | ID=MS.gene69740.t1.exon1;Parent=MS.gene69740.t1 |
| chr3.4 | CDS | 77023846 | 77024150 | 77023846 | ID=cds.MS.gene69740.t1;Parent=MS.gene69740.t1 |
| chr3.4 | exon | 77024484 | 77024577 | 77024484 | ID=MS.gene69740.t1.exon2;Parent=MS.gene69740.t1 |
| chr3.4 | CDS | 77024484 | 77024577 | 77024484 | ID=cds.MS.gene69740.t1;Parent=MS.gene69740.t1 |
| Gene Sequence |
| Protein sequence |
