AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056252


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056252.t1 MTR_1g114070 70.588 153 36 3 87 235 386 533 5.25e-62 209
MS.gene056252.t1 MTR_1g069565 57.669 163 55 4 80 235 298 453 2.37e-50 176
MS.gene056252.t1 MTR_1g069565 57.669 163 55 4 80 235 298 453 2.49e-50 176
MS.gene056252.t1 MTR_1g114150 56.410 156 64 3 80 235 497 648 1.04e-45 163
MS.gene056252.t1 MTR_1g114150 56.410 156 64 3 80 235 497 648 1.12e-45 162
MS.gene056252.t1 MTR_0020s0100 49.045 157 78 2 80 235 173 328 5.07e-42 151
MS.gene056252.t1 MTR_1g114130 54.730 148 63 4 89 235 490 634 5.21e-42 152
MS.gene056252.t1 MTR_5g047620 47.771 157 76 4 80 235 230 381 1.35e-32 125
MS.gene056252.t1 MTR_5g065200 42.177 147 84 1 89 235 304 449 9.49e-32 122
MS.gene056252.t1 MTR_8g062940 44.595 148 54 3 41 188 164 283 1.09e-26 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCATACTCAAGCTTAAT+TGG 0.220679 1.2:-84343543 None:intergenic
TCACATTCATTATCAGCTTT+TGG 0.248622 1.2:-84343705 None:intergenic
TGTCATTTCTTTGACATTGA+TGG 0.286327 1.2:-84342593 None:intergenic
ACTAACTCAGAGATGCAATT+TGG 0.317312 1.2:-84343762 None:intergenic
ATATCATCGGTGTACCTAAC+AGG 0.339047 1.2:-84343866 None:intergenic
CGCTGATTTAAGTTTCTTGT+GGG 0.366351 1.2:-84342435 None:intergenic
ATTCGTGAGTGGTCAAGATC+TGG 0.381498 1.2:+84343648 MS.gene056252:CDS
GAGAGGAGACTCGTGATAAT+AGG 0.388123 1.2:+84343828 MS.gene056252:CDS
GTTCTTGCTCGAACCCTGTT+AGG 0.393536 1.2:+84343852 MS.gene056252:CDS
CGAGGTCGCAATTATCTACA+TGG 0.434006 1.2:+84343567 MS.gene056252:CDS
GAAGTATCCTTCTCAGTTCA+AGG 0.464781 1.2:-84343737 None:intergenic
TCGCTGATTTAAGTTTCTTG+TGG 0.474100 1.2:-84342436 None:intergenic
ATCAATGTCAAAGAAATGAC+AGG 0.501804 1.2:+84342595 MS.gene056252:CDS
GCACATCATGTATACAAAAG+TGG 0.504161 1.2:+84342660 MS.gene056252:CDS
GTGTTTATTGTGTATATCAT+CGG 0.507594 1.2:-84343879 None:intergenic
GCTGATAATGAATGTGATCA+TGG 0.528933 1.2:+84343711 MS.gene056252:CDS
GTTGCTGCGAGCTCCGTGGT+GGG 0.531732 1.2:+84343499 MS.gene056252:CDS
ATGGTTTCCTTGAACTGAGA+AGG 0.533086 1.2:+84343730 MS.gene056252:CDS
CCGTGGTGGGAAGCTTCTAG+TGG 0.535084 1.2:+84343512 MS.gene056252:CDS
TTCGTGAGTGGTCAAGATCT+GGG 0.542666 1.2:+84343649 MS.gene056252:CDS
TAAGTTTCTTGTGGGACTTG+TGG 0.544227 1.2:-84342427 None:intergenic
TTAGATGCACAAAATGCAAA+CGG 0.550175 1.2:+84342628 MS.gene056252:CDS
AACTCAGAGATGCAATTTGG+AGG 0.551495 1.2:-84343759 None:intergenic
CGTGGTGGGAAGCTTCTAGT+GGG 0.559331 1.2:+84343513 MS.gene056252:CDS
TTGACATTGATGGCACATTG+AGG 0.559359 1.2:-84342583 None:intergenic
TGTTGCTGCGAGCTCCGTGG+TGG 0.562600 1.2:+84343498 MS.gene056252:CDS
ATGGTGTAGATCTACAGCCT+TGG 0.569025 1.2:-84343954 None:intergenic
TGTCAAAGAAATGACAGGGC+CGG 0.578080 1.2:+84342600 MS.gene056252:CDS
CACGGAGCTCGCAGCAACAG+AGG 0.580415 1.2:-84343494 None:intergenic
ACAATCAAGCTTCAGATGAG+AGG 0.585599 1.2:+84343811 MS.gene056252:CDS
TGGACAAGGAATGCTACCAT+CGG 0.593451 1.2:-84343685 None:intergenic
AATCAGCGAGAGTAATGCTA+AGG 0.598965 1.2:+84342450 MS.gene056252:CDS
AGAAAGTGTATCACATGCAG+AGG 0.606509 1.2:+84343614 MS.gene056252:CDS
ATACACAATAAACACAAGGA+AGG 0.616103 1.2:+84343887 MS.gene056252:CDS
CTCTGTTGCTGCGAGCTCCG+TGG 0.620459 1.2:+84343495 MS.gene056252:CDS
CCACTAGAAGCTTCCCACCA+CGG 0.634932 1.2:-84343512 None:intergenic
TATCATCGGTGTACCTAACA+GGG 0.654426 1.2:-84343865 None:intergenic
GGGTGGCATGCAAATGCCGA+TGG 0.671435 1.2:+84343669 MS.gene056252:CDS
ATGAGTCTACGATTCGTGAG+TGG 0.678627 1.2:+84343637 MS.gene056252:CDS
TCAATGTCAAAGAAATGACA+GGG 0.687101 1.2:+84342596 MS.gene056252:CDS
GCAGCAACAGAGGAGACCAA+AGG 0.694380 1.2:-84343484 None:intergenic
TGATATACACAATAAACACA+AGG 0.697614 1.2:+84343883 MS.gene056252:CDS
GGTCGCAATTATCTACATGG+TGG 0.698999 1.2:+84343570 MS.gene056252:CDS
GTGAGTGGTCAAGATCTGGG+TGG 0.773278 1.2:+84343652 MS.gene056252:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTATAATTTGATTTT+GGG + chr1.2:84343243-84343262 MS.gene056252:intron 10.0%
!!! TTCATTTTATAATTTGATTT+TGG + chr1.2:84343242-84343261 MS.gene056252:intron 10.0%
!! TAGATTTCAAATATAGACTA+CGG - chr1.2:84343025-84343044 None:intergenic 20.0%
!!! ATATTTGAAATCTATCCATT+TGG + chr1.2:84343031-84343050 MS.gene056252:intron 20.0%
! AAATCATACTCAAGCTTAAT+TGG - chr1.2:84343546-84343565 None:intergenic 25.0%
! ACAAAGTAAAACAGAAGTTA+AGG - chr1.2:84342693-84342712 None:intergenic 25.0%
! ATATCACTCTCTTTCAATTT+AGG - chr1.2:84342883-84342902 None:intergenic 25.0%
! ATTTCCTATAGTAAACCAAA+TGG - chr1.2:84343049-84343068 None:intergenic 25.0%
! GTGTTTATTGTGTATATCAT+CGG - chr1.2:84343882-84343901 None:intergenic 25.0%
! TATTGCACATATTACATGTT+TGG + chr1.2:84342798-84342817 MS.gene056252:intron 25.0%
! TCTCAAGCTAAATATAATAC+CGG + chr1.2:84343289-84343308 MS.gene056252:intron 25.0%
! TCTGCTCATTTATATAGTTT+GGG + chr1.2:84342492-84342511 MS.gene056252:intron 25.0%
! TGATATACACAATAAACACA+AGG + chr1.2:84343883-84343902 MS.gene056252:CDS 25.0%
! TGTAATATGTGCAATAACTT+CGG - chr1.2:84342794-84342813 None:intergenic 25.0%
!! CAAATTTTATATACTGTCCA+AGG + chr1.2:84343937-84343956 MS.gene056252:CDS 25.0%
!! CATATTACATGTTTGGATTT+TGG + chr1.2:84342805-84342824 MS.gene056252:intron 25.0%
!! TCATTTATATAGTTTGGGTT+TGG + chr1.2:84342497-84342516 MS.gene056252:intron 25.0%
!!! AATATGCCTTGTTTGATAAT+TGG + chr1.2:84342760-84342779 MS.gene056252:intron 25.0%
!!! ATATGCCTTGTTTGATAATT+GGG + chr1.2:84342761-84342780 MS.gene056252:intron 25.0%
!!! TTAATCAACAGTGTTTTGAA+GGG + chr1.2:84342555-84342574 MS.gene056252:intron 25.0%
AAGAATAGTTAATCGTTGGA+AGG - chr1.2:84342993-84343012 None:intergenic 30.0%
AGAATAGTTAATCGTTGGAA+GGG - chr1.2:84342992-84343011 None:intergenic 30.0%
ATACACAATAAACACAAGGA+AGG + chr1.2:84343887-84343906 MS.gene056252:CDS 30.0%
ATCAATGTCAAAGAAATGAC+AGG + chr1.2:84342595-84342614 MS.gene056252:CDS 30.0%
CTCTGCTCATTTATATAGTT+TGG + chr1.2:84342491-84342510 MS.gene056252:intron 30.0%
TACATCCCAATTATCAAACA+AGG - chr1.2:84342769-84342788 None:intergenic 30.0%
TCAATGTCAAAGAAATGACA+GGG + chr1.2:84342596-84342615 MS.gene056252:CDS 30.0%
TCAGAAGAATAGTTAATCGT+TGG - chr1.2:84342997-84343016 None:intergenic 30.0%
TGAAGAACAAATGATTGAGA+AGG + chr1.2:84343155-84343174 MS.gene056252:intron 30.0%
TTAGATGCACAAAATGCAAA+CGG + chr1.2:84342628-84342647 MS.gene056252:CDS 30.0%
! ATTTAGGATACCTTAGCAAA+AGG - chr1.2:84342867-84342886 None:intergenic 30.0%
! CACATTTTCTCTGACTATAT+AGG - chr1.2:84343207-84343226 None:intergenic 30.0%
! CTATCCATTTGGTTTACTAT+AGG + chr1.2:84343042-84343061 MS.gene056252:intron 30.0%
! TCACATTCATTATCAGCTTT+TGG - chr1.2:84343708-84343727 None:intergenic 30.0%
! TGTCATTTCTTTGACATTGA+TGG - chr1.2:84342596-84342615 None:intergenic 30.0%
!! CTTAATCAACAGTGTTTTGA+AGG + chr1.2:84342554-84342573 MS.gene056252:intron 30.0%
!!! ATTTTGGGATGTGAATTACA+GGG + chr1.2:84343258-84343277 MS.gene056252:intron 30.0%
!!! TAATCAACAGTGTTTTGAAG+GGG + chr1.2:84342556-84342575 MS.gene056252:intron 30.0%
!!! TTTTTTGTTGCCTTTTGCTA+AGG + chr1.2:84342854-84342873 MS.gene056252:intron 30.0%
ACTAACTCAGAGATGCAATT+TGG - chr1.2:84343765-84343784 None:intergenic 35.0%
ACTATAGGAAATGAAGCGTA+AGG + chr1.2:84343057-84343076 MS.gene056252:intron 35.0%
CAAGCTAAATATAATACCGG+CGG + chr1.2:84343292-84343311 MS.gene056252:intron 35.0%
GACTTCTTCCACATTTAAGA+CGG + chr1.2:84343128-84343147 MS.gene056252:intron 35.0%
GCACATCATGTATACAAAAG+TGG + chr1.2:84342660-84342679 MS.gene056252:CDS 35.0%
TTCATCAACCGTCTTAAATG+TGG - chr1.2:84343139-84343158 None:intergenic 35.0%
TTCTCTAAGTATCTGCTGAA+AGG + chr1.2:84343106-84343125 MS.gene056252:intron 35.0%
! AAGCTTGAGTATGATTTTCG+AGG + chr1.2:84343549-84343568 MS.gene056252:CDS 35.0%
! CAAAATGCTCTTTTGACCTT+TGG + chr1.2:84343468-84343487 MS.gene056252:CDS 35.0%
! GCTGATAATGAATGTGATCA+TGG + chr1.2:84343711-84343730 MS.gene056252:CDS 35.0%
! TCATTATCAGCTTTTGGACA+AGG - chr1.2:84343702-84343721 None:intergenic 35.0%
!! CGCTGATTTAAGTTTCTTGT+GGG - chr1.2:84342438-84342457 None:intergenic 35.0%
!! TCATCTTTAGAATAATCCGC+CGG - chr1.2:84343311-84343330 None:intergenic 35.0%
!! TCGCTGATTTAAGTTTCTTG+TGG - chr1.2:84342439-84342458 None:intergenic 35.0%
!!! AATCAACAGTGTTTTGAAGG+GGG + chr1.2:84342557-84342576 MS.gene056252:intron 35.0%
!!! GATTTTGGGATGTGAATTAC+AGG + chr1.2:84343257-84343276 MS.gene056252:intron 35.0%
AACTCAGAGATGCAATTTGG+AGG - chr1.2:84343762-84343781 None:intergenic 40.0%
AATCAGCGAGAGTAATGCTA+AGG + chr1.2:84342450-84342469 MS.gene056252:CDS 40.0%
ACAATCAAGCTTCAGATGAG+AGG + chr1.2:84343811-84343830 MS.gene056252:CDS 40.0%
ATAGTTAATCGTTGGAAGGG+TGG - chr1.2:84342989-84343008 None:intergenic 40.0%
ATATCATCGGTGTACCTAAC+AGG - chr1.2:84343869-84343888 None:intergenic 40.0%
ATGGTTTCCTTGAACTGAGA+AGG + chr1.2:84343730-84343749 MS.gene056252:CDS 40.0%
TAAGTTTCTTGTGGGACTTG+TGG - chr1.2:84342430-84342449 None:intergenic 40.0%
TATCATCGGTGTACCTAACA+GGG - chr1.2:84343868-84343887 None:intergenic 40.0%
TGTTCTGGTAAGGACTGATT+TGG + chr1.2:84343336-84343355 MS.gene056252:intron 40.0%
TTGCAATTATCACAGCACAC+AGG - chr1.2:84342919-84342938 None:intergenic 40.0%
! AGAAAGTGTATCACATGCAG+AGG + chr1.2:84343614-84343633 MS.gene056252:CDS 40.0%
! AGCATTTTGTACAGCTTCTG+TGG - chr1.2:84343457-84343476 None:intergenic 40.0%
! GAAGTATCCTTCTCAGTTCA+AGG - chr1.2:84343740-84343759 None:intergenic 40.0%
! TTGACATTGATGGCACATTG+AGG - chr1.2:84342586-84342605 None:intergenic 40.0%
! TTTTGTGCATCTAACAACGC+CGG - chr1.2:84342622-84342641 None:intergenic 40.0%
!! TTTGGGTTTGGTGTTATACG+CGG + chr1.2:84342509-84342528 MS.gene056252:intron 40.0%
ATGAGTCTACGATTCGTGAG+TGG + chr1.2:84343637-84343656 MS.gene056252:CDS 45.0%
ATTCGTGAGTGGTCAAGATC+TGG + chr1.2:84343648-84343667 MS.gene056252:CDS 45.0%
CGAGGTCGCAATTATCTACA+TGG + chr1.2:84343567-84343586 MS.gene056252:CDS 45.0%
CTAAAGATGAGCGTGTGTTC+TGG + chr1.2:84343321-84343340 MS.gene056252:intron 45.0%
GAGAGGAGACTCGTGATAAT+AGG + chr1.2:84343828-84343847 MS.gene056252:CDS 45.0%
GCAATTGCAAAGCATGCTTG+CGG + chr1.2:84342935-84342954 MS.gene056252:intron 45.0%
GGTCGCAATTATCTACATGG+TGG + chr1.2:84343570-84343589 MS.gene056252:CDS 45.0%
TATAGGAATACCTCGTCTCC+TGG - chr1.2:84343190-84343209 None:intergenic 45.0%
TGGACAAGGAATGCTACCAT+CGG - chr1.2:84343688-84343707 None:intergenic 45.0%
TGTCAAAGAAATGACAGGGC+CGG + chr1.2:84342600-84342619 MS.gene056252:CDS 45.0%
TTCGTGAGTGGTCAAGATCT+GGG + chr1.2:84343649-84343668 MS.gene056252:CDS 45.0%
TTGAGAAGGAAAGAGAAGCC+AGG + chr1.2:84343169-84343188 MS.gene056252:intron 45.0%
! GGTGTTATACGCGGTAATTG+TGG + chr1.2:84342518-84342537 MS.gene056252:intron 45.0%
AACTCAACCCTCTCTCGTGT+TGG - chr1.2:84343088-84343107 None:intergenic 50.0%
GATGAGCGTGTGTTCTGGTA+AGG + chr1.2:84343326-84343345 MS.gene056252:intron 50.0%
GTTCTTGCTCGAACCCTGTT+AGG + chr1.2:84343852-84343871 MS.gene056252:CDS 50.0%
ACAGCACACAGGCATGCTCT+TGG - chr1.2:84342908-84342927 None:intergenic 55.0%
AGGCTGAACCAACACGAGAG+AGG + chr1.2:84343077-84343096 MS.gene056252:intron 55.0%
CCACTAGAAGCTTCCCACCA+CGG - chr1.2:84343515-84343534 None:intergenic 55.0%
GAAAGAGAAGCCAGGAGACG+AGG + chr1.2:84343177-84343196 MS.gene056252:intron 55.0%
GCAGCAACAGAGGAGACCAA+AGG - chr1.2:84343487-84343506 None:intergenic 55.0%
GGCTGAACCAACACGAGAGA+GGG + chr1.2:84343078-84343097 MS.gene056252:intron 55.0%
GTGAGTGGTCAAGATCTGGG+TGG + chr1.2:84343652-84343671 MS.gene056252:CDS 55.0%
! CGTGGTGGGAAGCTTCTAGT+GGG + chr1.2:84343513-84343532 MS.gene056252:CDS 55.0%
GGGTGGCATGCAAATGCCGA+TGG + chr1.2:84343669-84343688 MS.gene056252:CDS 60.0%
! CCGTGGTGGGAAGCTTCTAG+TGG + chr1.2:84343512-84343531 MS.gene056252:CDS 60.0%
CACGGAGCTCGCAGCAACAG+AGG - chr1.2:84343497-84343516 None:intergenic 65.0%
CTCTGTTGCTGCGAGCTCCG+TGG + chr1.2:84343495-84343514 MS.gene056252:CDS 65.0%
GTTGCTGCGAGCTCCGTGGT+GGG + chr1.2:84343499-84343518 MS.gene056252:CDS 65.0%
TGTTGCTGCGAGCTCCGTGG+TGG + chr1.2:84343498-84343517 MS.gene056252:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 84342419 84343965 84342419 ID=MS.gene056252
chr1.2 mRNA 84342419 84343965 84342419 ID=MS.gene056252.t1;Parent=MS.gene056252
chr1.2 exon 84342419 84342471 84342419 ID=MS.gene056252.t1.exon1;Parent=MS.gene056252.t1
chr1.2 CDS 84342419 84342471 84342419 ID=cds.MS.gene056252.t1;Parent=MS.gene056252.t1
chr1.2 exon 84342566 84342681 84342566 ID=MS.gene056252.t1.exon2;Parent=MS.gene056252.t1
chr1.2 CDS 84342566 84342681 84342566 ID=cds.MS.gene056252.t1;Parent=MS.gene056252.t1
chr1.2 exon 84343424 84343965 84343424 ID=MS.gene056252.t1.exon3;Parent=MS.gene056252.t1
chr1.2 CDS 84343424 84343965 84343424 ID=cds.MS.gene056252.t1;Parent=MS.gene056252.t1
Gene Sequence

>MS.gene056252

ATGAGGTTCCACAAGTCCCACAAGAAACTTAAATCAGCGAGAGTAATGCTAAGGTACATTACACGATCATCACTCTGCTCATTTATATAGTTTGGGTTTGGTGTTATACGCGGTAATTGTGGTTAGTTAACGTTTCTTAATCAACAGTGTTTTGAAGGGGGTTTCCTCAATGTGCCATCAATGTCAAAGAAATGACAGGGCCGGCGTTGTTAGATGCACAAAATGCAAACGGAAAGATTTTGCACATCATGTATACAAAAGTGGTACAACTCCTTAACTTCTGTTTTACTTTGTTTAACTTGCTTCATCTATGTTGTGTCAGTTTGTTCTATTGTTTTTTGAATATGCCTTGTTTGATAATTGGGATGTAGACCGAAGTTATTGCACATATTACATGTTTGGATTTTGGTTGATGAAATATTGATCAAACTATGCTTTTTTGTTGCCTTTTGCTAAGGTATCCTAAATTGAAAGAGAGTGATATTGCCAAGAGCATGCCTGTGTGCTGTGATAATTGCAATTGCAAAGCATGCTTGCGGTCTTTTAAACTCAATCAATGTCAGTGATCCACCCTTCCAACGATTAACTATTCTTCTGATTTTTCCGTAGTCTATATTTGAAATCTATCCATTTGGTTTACTATAGGAAATGAAGCGTAAGGCTGAACCAACACGAGAGAGGGTTGAGTTCTCTAAGTATCTGCTGAAAGGACTTCTTCCACATTTAAGACGGTTGATGAAGAACAAATGATTGAGAAGGAAAGAGAAGCCAGGAGACGAGGTATTCCTATATAGTCAGAGAAAATGTGTAGTTTCATATATTTTTCATTTTATAATTTGATTTTGGGATGTGAATTACAGGGCTCTCAACTCTCAAGCTAAATATAATACCGGCGGATTATTCTAAAGATGAGCGTGTGTTCTGGTAAGGACTGATTTGGTTATTACTCAATGCTTTTTTTACTATTCTAATGCTATATAGTTGTTGACTTCACACTTTTTGTAGTGACAACTGTAAAACATCAATATTTGACTACCACAGAAGCTGTACAAAATGCTCTTTTGACCTTTGGTCTCCTCTGTTGCTGCGAGCTCCGTGGTGGGAAGCTTCTAGTGGGTGCAGATCCAATTAAGCTTGAGTATGATTTTCGAGGTCGCAATTATCTACATGGTGGAAATGAAGAAAAACATGTTAAAGAAAGTGTATCACATGCAGAGGATGAGTCTACGATTCGTGAGTGGTCAAGATCTGGGTGGCATGCAAATGCCGATGGTAGCATTCCTTGTCCAAAAGCTGATAATGAATGTGATCATGGTTTCCTTGAACTGAGAAGGATACTTCCTCCAAATTGCATCTCTGAGTTAGTTTGTAAAGCAAACAAACTTGCAGAAAACAATCAAGCTTCAGATGAGAGGAGACTCGTGATAATAGGTGTTCTTGCTCGAACCCTGTTAGGTACACCGATGATATACACAATAAACACAAGGAAGGCAGCTTTTTCAATGAAGATTCAAGTGACAAATTTTATATACTGTCCAAGGCTGTAG

Protein sequence

>MS.gene056252.t1

MRFHKSHKKLKSARVMLSVLKGVSSMCHQCQRNDRAGVVRCTKCKRKDFAHHVYKSVTTVKHQYLTTTEAVQNALLTFGLLCCCELRGGKLLVGADPIKLEYDFRGRNYLHGGNEEKHVKESVSHAEDESTIREWSRSGWHANADGSIPCPKADNECDHGFLELRRILPPNCISELVCKANKLAENNQASDERRLVIIGVLARTLLGTPMIYTINTRKAAFSMKIQVTNFIYCPRL