AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69889

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69889.t1 MTR_3g079560 95.758 495 20 1 1 494 1 495 0.0 956
MS.gene69889.t1 MTR_3g079570 91.129 496 41 3 1 494 1 495 0.0 906
MS.gene69889.t1 MTR_3g079600 87.575 499 55 4 1 494 1 497 0.0 885
MS.gene69889.t1 MTR_3g079590 89.315 496 50 3 1 494 1 495 0.0 882
MS.gene69889.t1 MTR_3g079630 84.813 507 61 6 1 494 1 504 0.0 861
MS.gene69889.t1 MTR_3g079620 85.487 503 62 3 1 493 1 502 0.0 855
MS.gene69889.t1 MTR_1g020090 62.236 474 171 5 18 489 30 497 0.0 592
MS.gene69889.t1 MTR_1g021010 60.861 488 183 7 10 493 7 490 0.0 575
MS.gene69889.t1 MTR_1g021020 59.915 469 182 5 27 493 27 491 0.0 564
MS.gene69889.t1 MTR_3g034290 53.441 494 204 10 11 489 8 490 1.82e-175 503
MS.gene69889.t1 MTR_0003s0560 55.093 432 183 7 65 490 62 488 1.55e-161 467
MS.gene69889.t1 MTR_1g095560 51.570 446 198 6 57 491 20 458 5.55e-159 459
MS.gene69889.t1 MTR_1g095540 52.900 431 190 7 73 491 29 458 1.32e-153 446
MS.gene69889.t1 MTR_1g095560 48.745 478 195 8 57 491 20 490 4.67e-153 445
MS.gene69889.t1 MTR_4g075150 50.993 453 194 12 59 492 21 464 6.39e-151 439
MS.gene69889.t1 MTR_4g075150 50.549 455 195 11 59 492 21 466 1.65e-150 438
MS.gene69889.t1 MTR_2g062990 49.644 421 202 4 80 490 43 463 3.48e-149 435
MS.gene69889.t1 MTR_2g063000 48.526 441 213 6 62 490 26 464 4.77e-149 434
MS.gene69889.t1 MTR_2g062980 50.356 421 199 5 80 490 43 463 7.21e-149 434
MS.gene69889.t1 MTR_2g081620 48.941 425 206 6 73 486 36 460 1.07e-145 426
MS.gene69889.t1 MTR_7g013790 49.315 438 202 5 70 489 29 464 2.05e-145 425
MS.gene69889.t1 MTR_3g034290 52.913 412 169 9 11 408 8 408 1.82e-142 415
MS.gene69889.t1 MTR_4g089155 48.984 443 195 10 74 491 51 487 9.76e-141 414
MS.gene69889.t1 MTR_4g122950 46.413 446 211 6 73 491 35 479 4.56e-139 409
MS.gene69889.t1 MTR_2g012860 47.955 440 204 9 73 490 38 474 6.69e-135 398
MS.gene69889.t1 MTR_2g012850 46.682 437 210 7 75 491 34 467 2.31e-134 397
MS.gene69889.t1 MTR_4g122960 44.595 444 220 6 73 491 35 477 1.89e-131 390
MS.gene69889.t1 MTR_2g063000 43.991 441 202 7 62 490 26 433 6.36e-125 372
MS.gene69889.t1 MTR_3g090130 46.501 443 212 11 69 491 21 458 8.50e-123 367
MS.gene69889.t1 MTR_3g090150 44.796 442 223 9 69 491 26 465 1.47e-119 359
MS.gene69889.t1 MTR_3g498755 44.545 440 218 10 72 489 10 445 5.73e-118 354
MS.gene69889.t1 MTR_5g072570 44.944 445 220 9 69 491 22 463 6.45e-118 355
MS.gene69889.t1 MTR_2g449850 43.341 443 214 11 73 491 35 464 1.25e-111 338
MS.gene69889.t1 MTR_7g021130 41.935 434 228 9 75 489 29 457 3.20e-109 332
MS.gene69889.t1 MTR_8g099930 42.308 442 225 12 73 491 30 464 5.05e-109 332
MS.gene69889.t1 MTR_0003s0560 59.259 270 107 3 65 334 62 328 5.28e-109 328
MS.gene69889.t1 MTR_3g434850 41.441 444 220 11 71 488 25 454 1.23e-106 325
MS.gene69889.t1 MTR_1g040370 39.765 425 214 9 72 489 26 415 2.77e-103 315
MS.gene69889.t1 MTR_1g102130 35.955 445 219 16 64 483 81 484 2.15e-74 243
MS.gene69889.t1 MTR_7g080180 35.135 481 243 17 69 485 20 495 8.30e-73 239
MS.gene69889.t1 MTR_8g011350 33.619 467 249 9 77 486 31 493 2.77e-72 238
MS.gene69889.t1 MTR_7g051430 32.165 485 264 10 58 485 16 492 1.09e-65 220
MS.gene69889.t1 MTR_8g011350 32.579 442 237 9 77 461 31 468 4.68e-62 210
MS.gene69889.t1 MTR_7g080180 33.996 453 227 17 69 458 20 463 3.39e-61 207
MS.gene69889.t1 MTR_1g050545 31.455 426 247 11 89 490 81 485 1.49e-56 196
MS.gene69889.t1 MTR_4g057585 31.519 441 234 15 82 487 87 494 1.59e-55 193
MS.gene69889.t1 MTR_4g057595 30.822 438 231 15 91 491 97 499 2.57e-54 190
MS.gene69889.t1 MTR_4g021530 30.435 437 265 15 76 483 39 465 2.40e-53 187
MS.gene69889.t1 MTR_2g095730 29.490 451 268 15 74 494 73 503 1.59e-52 185
MS.gene69889.t1 MTR_1g094155 30.445 427 251 13 88 490 87 491 3.88e-52 184
MS.gene69889.t1 MTR_2g095750 30.337 445 253 16 75 487 80 499 4.08e-52 184
MS.gene69889.t1 MTR_4g021520 30.455 440 265 14 74 483 37 465 5.32e-51 181
MS.gene69889.t1 MTR_2g018590 29.330 433 273 11 80 483 36 464 3.39e-50 178
MS.gene69889.t1 MTR_4g057570 31.019 432 240 14 88 487 86 491 5.85e-50 178
MS.gene69889.t1 MTR_2g018600 29.224 438 266 14 80 483 37 464 7.19e-50 177
MS.gene69889.t1 MTR_4g021535 29.545 440 269 16 74 483 33 461 1.23e-49 177
MS.gene69889.t1 MTR_4g021490 29.977 437 271 15 74 483 37 465 1.60e-49 176
MS.gene69889.t1 MTR_4g021500 30.385 441 262 16 74 482 37 464 1.67e-49 182
MS.gene69889.t1 MTR_4g021500 29.540 413 256 15 89 483 487 882 2.65e-42 161
MS.gene69889.t1 MTR_4g021500 30.385 441 262 16 74 482 37 464 2.65e-49 182
MS.gene69889.t1 MTR_4g021500 28.706 425 262 14 89 483 487 900 1.57e-41 159
MS.gene69889.t1 MTR_4g057600 29.717 424 252 12 88 487 92 493 8.33e-49 175
MS.gene69889.t1 MTR_4g021510 31.193 436 261 16 76 482 38 463 9.96e-49 174
MS.gene69889.t1 MTR_7g051430 36.934 287 169 4 58 339 16 295 5.94e-48 171
MS.gene69889.t1 MTR_5g097180 30.288 416 252 12 93 487 43 441 1.56e-47 171
MS.gene69889.t1 MTR_3g100420 30.752 452 262 17 73 483 50 491 2.52e-46 168
MS.gene69889.t1 MTR_2g018630 29.907 428 269 13 80 483 40 460 4.92e-45 164
MS.gene69889.t1 MTR_3g045770 27.546 432 287 12 77 487 27 453 8.86e-42 155
MS.gene69889.t1 MTR_2g009200 30.856 444 241 15 74 484 25 435 1.00e-41 154
MS.gene69889.t1 MTR_4g021520 30.025 403 242 13 74 447 37 428 1.47e-41 154
MS.gene69889.t1 MTR_2g095710 29.177 401 241 13 106 487 106 482 2.52e-40 152
MS.gene69889.t1 MTR_4g084710 30.282 426 254 16 93 494 45 451 4.63e-39 147
MS.gene69889.t1 MTR_0075s0010 30.056 356 185 12 75 422 6 305 9.15e-37 138
MS.gene69889.t1 MTR_4g021535 26.136 440 237 18 74 483 33 414 3.81e-32 127
MS.gene69889.t1 MTR_2g018630 27.485 342 223 11 161 483 20 355 2.64e-27 113
MS.gene69889.t1 MTR_4g056520 27.294 436 253 19 94 483 36 453 4.21e-25 108
MS.gene69889.t1 MTR_1g051175 49.425 87 41 2 83 168 18 102 2.65e-20 91.3
MS.gene69889.t1 MTR_7g100560 29.493 217 86 6 87 303 68 217 4.88e-18 85.5
MS.gene69889.t1 MTR_2g084530 25.339 442 248 15 94 481 36 449 5.76e-17 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69889.t1 AT3G63470 52.966 472 200 8 25 483 29 491 1.32e-174 501
MS.gene69889.t1 AT4G30610 52.283 438 190 5 72 491 28 464 4.61e-157 454
MS.gene69889.t1 AT2G24010 51.981 429 194 6 73 491 27 453 2.57e-154 447
MS.gene69889.t1 AT3G07990 52.607 422 185 8 75 483 32 451 7.01e-152 441
MS.gene69889.t1 AT2G24000 49.885 435 196 6 72 486 26 458 1.55e-149 435
MS.gene69889.t1 AT2G24010 50.000 444 195 7 73 491 27 468 9.67e-149 434
MS.gene69889.t1 AT2G35780 51.174 426 189 7 72 483 25 445 7.21e-148 431
MS.gene69889.t1 AT5G23210 47.609 460 194 8 72 492 47 498 3.01e-146 428
MS.gene69889.t1 AT2G35770 49.330 448 217 6 54 493 17 462 2.87e-145 424
MS.gene69889.t1 AT2G24010 47.527 465 192 8 77 491 2 464 1.40e-142 417
MS.gene69889.t1 AT4G30810 47.166 441 218 5 68 494 27 466 4.73e-142 417
MS.gene69889.t1 AT3G02110 48.322 447 204 6 69 491 30 473 1.13e-141 416
MS.gene69889.t1 AT1G61130 49.195 435 201 8 75 491 30 462 1.72e-138 407
MS.gene69889.t1 AT1G11080 46.154 455 215 8 66 491 38 491 3.86e-137 405
MS.gene69889.t1 AT1G11080 46.154 455 215 8 66 491 38 491 3.98e-137 405
MS.gene69889.t1 AT5G08260 46.712 441 205 10 70 483 33 470 2.00e-133 395
MS.gene69889.t1 AT4G15100 46.517 445 209 9 73 491 41 482 3.39e-131 389
MS.gene69889.t1 AT3G52000 48.712 427 202 10 75 491 62 481 3.63e-131 389
MS.gene69889.t1 AT3G17180 46.085 447 213 9 72 492 34 478 6.12e-131 388
MS.gene69889.t1 AT3G52020 44.554 505 252 15 5 492 8 501 3.41e-130 387
MS.gene69889.t1 AT2G05850 45.977 435 217 9 71 492 58 487 5.53e-129 384
MS.gene69889.t1 AT5G23210 46.973 413 172 8 72 445 47 451 1.86e-127 379
MS.gene69889.t1 AT5G23210 45.854 410 176 7 122 492 1 403 9.50e-122 362
MS.gene69889.t1 AT1G43780 44.866 448 220 11 69 492 30 474 2.46e-121 364
MS.gene69889.t1 AT3G52010 45.183 436 212 12 73 491 61 486 4.01e-121 363
MS.gene69889.t1 AT5G23210 46.020 402 171 7 130 492 8 402 1.93e-120 359
MS.gene69889.t1 AT2G12480 45.125 441 221 10 69 492 24 460 8.40e-119 357
MS.gene69889.t1 AT5G42240 43.596 445 226 9 69 491 25 466 1.51e-114 346
MS.gene69889.t1 AT2G33530 44.444 441 217 11 71 489 28 462 1.84e-114 346
MS.gene69889.t1 AT1G28110 43.478 437 221 10 73 489 28 458 8.47e-113 341
MS.gene69889.t1 AT1G28110 43.478 437 221 10 73 489 28 458 8.47e-113 341
MS.gene69889.t1 AT2G12480 43.218 435 214 9 69 492 24 436 6.60e-112 338
MS.gene69889.t1 AT5G42230 42.793 444 229 9 69 490 21 461 2.38e-111 338
MS.gene69889.t1 AT5G42230 42.793 444 229 9 69 490 21 461 2.38e-111 338
MS.gene69889.t1 AT3G17180 44.030 402 198 8 72 448 34 433 6.94e-111 335
MS.gene69889.t1 AT2G12480 42.989 435 213 9 69 492 24 434 8.76e-111 335
MS.gene69889.t1 AT2G24010 48.459 357 157 7 77 408 2 356 2.48e-110 331
MS.gene69889.t1 AT2G12480 42.922 438 210 10 69 492 24 435 5.53e-109 331
MS.gene69889.t1 AT5G23210 45.070 355 149 7 130 445 8 355 1.27e-101 309
MS.gene69889.t1 AT4G12910 36.000 475 242 10 66 486 29 495 1.34e-81 262
MS.gene69889.t1 AT3G25420 34.100 478 247 12 66 485 24 491 5.21e-75 244
MS.gene69889.t1 AT3G56540 64.481 183 59 3 53 231 52 232 1.24e-74 236
MS.gene69889.t1 AT2G24000 43.165 278 138 4 228 486 119 395 4.07e-71 232
MS.gene69889.t1 AT2G24000 59.140 93 36 2 72 163 26 117 8.92e-28 115
MS.gene69889.t1 AT4G12910 34.483 435 224 9 66 447 29 455 1.87e-67 224
MS.gene69889.t1 AT2G22990 32.251 431 255 13 74 486 20 431 4.29e-60 204
MS.gene69889.t1 AT3G10410 32.019 431 244 13 82 487 90 496 1.19e-57 199
MS.gene69889.t1 AT3G45010 30.892 437 243 13 82 487 92 500 1.43e-56 196
MS.gene69889.t1 AT2G23000 33.645 428 255 14 74 486 22 435 3.09e-56 194
MS.gene69889.t1 AT2G23000 33.645 428 255 14 74 486 22 435 3.09e-56 194
MS.gene69889.t1 AT2G22990 32.227 422 250 12 74 477 20 423 3.42e-56 194
MS.gene69889.t1 AT2G22990 32.678 407 238 12 74 462 20 408 4.77e-56 192
MS.gene69889.t1 AT5G22980 28.931 477 274 16 35 487 60 495 3.51e-54 190
MS.gene69889.t1 AT3G10450 31.702 429 258 11 74 486 26 435 3.60e-54 188
MS.gene69889.t1 AT2G22970 33.014 418 253 10 80 485 28 430 3.11e-53 186
MS.gene69889.t1 AT2G22990 30.858 431 252 14 74 486 20 422 3.10e-52 182
MS.gene69889.t1 AT2G23010 31.591 421 259 11 80 485 28 434 4.45e-52 182
MS.gene69889.t1 AT2G22920 32.706 425 247 13 80 485 28 432 5.75e-52 182
MS.gene69889.t1 AT1G73300 31.163 430 259 11 74 486 30 439 8.50e-52 182
MS.gene69889.t1 AT1G73300 31.163 430 259 11 74 486 30 439 8.50e-52 182
MS.gene69889.t1 AT2G23010 31.354 421 260 11 80 485 28 434 2.13e-51 181
MS.gene69889.t1 AT3G10450 31.618 408 244 11 95 486 4 392 2.84e-51 179
MS.gene69889.t1 AT1G33540 31.707 451 266 14 68 485 20 461 4.59e-51 181
MS.gene69889.t1 AT1G73270 30.575 435 267 12 68 486 24 439 5.47e-51 180
MS.gene69889.t1 AT1G73270 30.575 435 267 12 68 486 24 439 6.43e-51 180
MS.gene69889.t1 AT5G36180 31.395 430 258 11 74 486 30 439 2.47e-50 178
MS.gene69889.t1 AT1G73280 31.507 438 259 12 68 486 24 439 5.20e-50 177
MS.gene69889.t1 AT2G22970 33.501 397 238 9 80 464 28 410 1.41e-49 176
MS.gene69889.t1 AT2G22970 33.333 396 238 9 80 463 28 409 1.54e-49 175
MS.gene69889.t1 AT2G22980 31.490 416 254 11 80 483 29 425 1.60e-49 176
MS.gene69889.t1 AT3G12220 31.043 422 266 10 77 487 27 434 3.18e-49 175
MS.gene69889.t1 AT1G33540 31.840 424 247 14 95 485 4 418 7.96e-49 173
MS.gene69889.t1 AT3G10450 31.527 406 244 10 74 463 26 413 1.45e-48 172
MS.gene69889.t1 AT3G12203 30.238 420 267 11 77 485 9 413 6.49e-48 171
MS.gene69889.t1 AT3G12203 30.166 421 268 11 77 486 30 435 8.58e-48 171
MS.gene69889.t1 AT1G73310 30.626 431 260 11 74 486 30 439 1.31e-47 171
MS.gene69889.t1 AT1G73290 30.787 432 268 13 68 486 23 436 1.31e-47 171
MS.gene69889.t1 AT5G09640 30.022 453 256 16 77 485 27 462 4.68e-47 170
MS.gene69889.t1 AT2G22920 32.905 389 224 11 80 450 28 397 7.39e-47 168
MS.gene69889.t1 AT3G12230 30.332 422 265 13 77 485 27 432 1.04e-46 168
MS.gene69889.t1 AT3G12220 30.641 421 264 11 78 487 1 404 1.58e-46 167
MS.gene69889.t1 AT2G22980 31.818 396 240 10 80 463 29 406 2.55e-46 166
MS.gene69889.t1 AT2G22980 31.899 395 239 10 80 462 29 405 3.45e-46 167
MS.gene69889.t1 AT3G12240 30.714 420 266 14 77 485 28 433 4.74e-46 166
MS.gene69889.t1 AT3G10450 31.429 385 230 10 95 463 4 370 1.21e-45 164
MS.gene69889.t1 AT3G12203 30.179 391 247 11 106 485 1 376 3.47e-44 160
MS.gene69889.t1 AT1G73290 30.633 395 245 11 68 450 23 400 3.55e-43 158
MS.gene69889.t1 AT1G15000 30.073 409 248 15 93 485 37 423 8.96e-43 157
MS.gene69889.t1 AT1G73310 30.789 393 235 9 74 449 30 402 1.29e-42 157
MS.gene69889.t1 AT3G12220 30.216 417 224 11 77 487 27 382 5.22e-42 154
MS.gene69889.t1 AT2G22970 36.260 262 155 6 80 333 28 285 3.45e-38 142
MS.gene69889.t1 AT2G22920 35.741 263 156 5 80 333 28 286 4.22e-38 142
MS.gene69889.t1 AT1G73290 35.065 231 143 3 68 293 23 251 5.89e-38 141
MS.gene69889.t1 AT2G22990 30.448 335 202 11 165 486 1 317 2.77e-37 139
MS.gene69889.t1 AT2G22990 30.149 335 203 11 165 486 1 317 1.39e-36 137
MS.gene69889.t1 AT1G73280 34.545 275 167 5 68 333 24 294 1.55e-36 139
MS.gene69889.t1 AT2G22980 36.122 263 152 6 80 333 29 284 6.08e-36 137
MS.gene69889.t1 AT5G36180 29.360 344 212 9 154 485 12 336 7.95e-31 122
MS.gene69889.t1 AT5G36180 29.360 344 212 9 154 485 12 336 7.95e-31 122
MS.gene69889.t1 AT1G73280 29.107 347 211 10 154 486 12 337 5.11e-30 120
MS.gene69889.t1 AT5G22960 35.227 176 92 3 82 257 25 178 1.32e-27 109
MS.gene69889.t1 AT1G73280 29.091 330 205 10 168 486 1 312 2.54e-27 112
MS.gene69889.t1 AT5G36180 28.571 322 200 8 154 463 12 315 6.55e-26 108
MS.gene69889.t1 AT2G22980 28.758 306 194 8 165 463 1 289 2.42e-24 103
MS.gene69889.t1 AT1G73310 28.571 308 189 7 154 449 12 300 4.57e-24 103
MS.gene69889.t1 AT5G36180 28.664 307 190 7 154 449 12 300 1.11e-23 102
MS.gene69889.t1 AT2G27920 26.376 436 257 18 94 482 37 455 8.64e-23 101
MS.gene69889.t1 AT2G22920 27.881 269 162 10 231 485 3 253 6.83e-13 69.3

Find 116 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTAGTATTGTGGCTTAA+TGG 0.193981 3.4:-75881641 MS.gene69889:CDS
ATTACTCAACTTGAGTTTAT+TGG 0.209420 3.4:-75881972 MS.gene69889:CDS
GCTTGACTAGCTTTGCTATA+TGG 0.232639 3.4:+75881941 None:intergenic
GACCCTTGTTCTGATTATTA+TGG 0.237969 3.4:-75880277 MS.gene69889:CDS
ACTGGGGTATGGAGCCTTTC+AGG 0.238241 3.4:-75881100 MS.gene69889:CDS
TGATGATGCCACAAGTTTAA+AGG 0.260603 3.4:-75880530 MS.gene69889:CDS
ACAGTTGGTAAGATGGTAAT+TGG 0.266699 3.4:+75879762 None:intergenic
GGTTTAGTTGAAGGGTTATA+TGG 0.270576 3.4:+75881662 None:intergenic
GGACGCCAAGCTGCGTTTAT+AGG 0.274588 3.4:+75879479 None:intergenic
CTTAGAACCAATTAATTTGA+TGG 0.278306 3.4:+75879070 None:intergenic
TCGATAGCGGCATTAAATTA+TGG 0.305820 3.4:-75879728 MS.gene69889:CDS
CTGCGTTTATAGGGAGCTTA+AGG 0.310250 3.4:+75879489 None:intergenic
TTCATGCAAAACCTACAAAT+TGG 0.311190 3.4:-75880216 MS.gene69889:CDS
TATACCGTAACCTGTATTCT+TGG 0.320202 3.4:-75881038 MS.gene69889:CDS
CTGCAATGGGTCCAATTTGT+AGG 0.327490 3.4:+75880205 None:intergenic
AGGACCTGGCTGTTCCTCAC+TGG 0.332819 3.4:-75881118 MS.gene69889:CDS
GCTTGGCGTCCATGGTATTC+TGG 0.333942 3.4:-75879467 MS.gene69889:CDS
ATCCTCCCAAGACTCTTTCT+TGG 0.339640 3.4:-75881877 MS.gene69889:CDS
GATGATGCCACAAGTTTAAA+GGG 0.364178 3.4:-75880529 MS.gene69889:CDS
ATCATTGTCAAAGCCCGTTC+TGG 0.370318 3.4:+75879130 None:intergenic
ATGATCTCATCATTCCTCTA+TGG 0.384946 3.4:-75879112 MS.gene69889:CDS
GACGCCAAGCTGCGTTTATA+GGG 0.390982 3.4:+75879480 None:intergenic
TCCAAGCTGGCAACCAGAAC+GGG 0.395617 3.4:-75879143 MS.gene69889:CDS
GCCCGTTCTGGTTGCCAGCT+TGG 0.397914 3.4:+75879142 None:intergenic
TTTATTGTTGTGAAGAATAG+TGG 0.404755 3.4:+75880701 None:intergenic
CACCTTCATTCCAAGAATAC+AGG 0.411785 3.4:+75881028 None:intergenic
GGCTGTTCCTCACTGGGGTA+TGG 0.417363 3.4:-75881111 MS.gene69889:CDS
AACCTCTGGTCTATTTAGAT+AGG 0.422418 3.4:+75880249 None:intergenic
AATTTCAGATAGGGAATGCT+TGG 0.423855 3.4:-75880555 MS.gene69889:intron
CAAGACTCTTTCTTGGGAAG+TGG 0.424295 3.4:-75881870 MS.gene69889:CDS
TATGAAAATCCCACTCCTGC+TGG 0.427452 3.4:+75880888 None:intergenic
TCCTCCCAAGACTCTTTCTT+GGG 0.428322 3.4:-75881876 MS.gene69889:CDS
GCATTAAATTATGGATATAC+AGG 0.431413 3.4:-75879719 MS.gene69889:intron
AACATACAGAAATGCCTTTG+AGG 0.436367 3.4:+75880655 None:intergenic
CCTATGTCTTCCTCATCAAC+TGG 0.443865 3.4:-75880809 MS.gene69889:CDS
TGCGTTTATAGGGAGCTTAA+GGG 0.446999 3.4:+75879490 None:intergenic
TTGCTCAAAATTCACTCCAT+AGG 0.448912 3.4:+75881751 None:intergenic
AATTAATTTGATGGTGAACT+AGG 0.449264 3.4:+75879079 None:intergenic
CTACAAATTGGACCCATTGC+AGG 0.456745 3.4:-75880204 MS.gene69889:intron
CACTTCCCAAGAAAGAGTCT+TGG 0.457536 3.4:+75881871 None:intergenic
AATACTAAAGGTTTAGTTGA+AGG 0.459170 3.4:+75881653 None:intergenic
GGACCTGGCTGTTCCTCACT+GGG 0.459617 3.4:-75881117 MS.gene69889:CDS
CTAAAGGCCTTCTTTGAACA+TGG 0.467756 3.4:-75880388 MS.gene69889:CDS
CAACTAAACCTTTAGTATTG+TGG 0.468364 3.4:-75881649 MS.gene69889:CDS
ATCTCCTGATTTGTCATAGT+CGG 0.468625 3.4:+75880851 None:intergenic
GACAAGATTGTCACATTGCC+TGG 0.468983 3.4:-75881779 MS.gene69889:CDS
ACTTCCCAAGAAAGAGTCTT+GGG 0.474421 3.4:+75881872 None:intergenic
GAGGAAGACATAGGCATCCT+TGG 0.475321 3.4:+75880818 None:intergenic
TATCTTTCCATGTTCAAAGA+AGG 0.476920 3.4:+75880381 None:intergenic
ACATTGCCTGGTCAACCCTA+TGG 0.477819 3.4:-75881767 MS.gene69889:CDS
CATTCCAAGAATACAGGTTA+CGG 0.481730 3.4:+75881034 None:intergenic
TGCTCAAAATTCACTCCATA+GGG 0.485269 3.4:+75881752 None:intergenic
AAATAGACCAGAGGTTCAAA+AGG 0.494500 3.4:-75880242 MS.gene69889:CDS
ATACTAAAGGTTTAGTTGAA+GGG 0.494760 3.4:+75881654 None:intergenic
TAAACGCAGCTTGGCGTCCA+TGG 0.502210 3.4:-75879475 MS.gene69889:CDS
TATAGAATATTACCTGCAAT+GGG 0.505247 3.4:+75880192 None:intergenic
TTATAGAATATTACCTGCAA+TGG 0.506360 3.4:+75880191 None:intergenic
TATCAATAATGTAACACTAA+AGG 0.509465 3.4:-75880404 MS.gene69889:CDS
CCATTAAGCCACAATACTAA+AGG 0.509726 3.4:+75881641 None:intergenic
GTCACTGTTGATCCTGAGGC+TGG 0.511770 3.4:-75881716 MS.gene69889:CDS
GCTGATGTTGCACCTCAAGA+GGG 0.512467 3.4:-75881815 MS.gene69889:CDS
AACCTGTATTCTTGGAATGA+AGG 0.514399 3.4:-75881030 MS.gene69889:intron
ACATCCGACTATGACAAATC+AGG 0.514877 3.4:-75880855 MS.gene69889:CDS
TTTGTTACAGTGAGAGGAGC+AGG 0.515378 3.4:-75879175 MS.gene69889:CDS
TGTCTTCCTCATCAACTGGC+TGG 0.515449 3.4:-75880805 MS.gene69889:CDS
AAAAGCTCTCTTCCAGCCTC+AGG 0.516852 3.4:+75881704 None:intergenic
CCATCAGAGTTGATTCTGAA+GGG 0.523486 3.4:+75881069 None:intergenic
TGCTGATGTTGCACCTCAAG+AGG 0.525644 3.4:-75881816 MS.gene69889:CDS
TCCATGGTATTCTGGAAAAG+AGG 0.526540 3.4:-75879459 MS.gene69889:intron
GCCATCAGAGTTGATTCTGA+AGG 0.530541 3.4:+75881068 None:intergenic
ATACTTGACAGTTGGTAAGA+TGG 0.530659 3.4:+75879755 None:intergenic
AGTTGAGAACAAGATGAAGA+AGG 0.533495 3.4:-75882014 None:intergenic
TTGACATTTGTTACAGTGAG+AGG 0.534371 3.4:-75879181 MS.gene69889:CDS
TTCACTCCATAGGGTTGACC+AGG 0.534868 3.4:+75881761 None:intergenic
CCACTCCTGCTGGAGACTCT+AGG 0.536283 3.4:+75880898 None:intergenic
ATGACCAGCATAACTCTCTC+CGG 0.536968 3.4:+75880743 None:intergenic
ATAGTCGTCTTCTTACTGAC+TGG 0.537929 3.4:-75879794 MS.gene69889:intron
CCTAGAGTCTCCAGCAGGAG+TGG 0.545565 3.4:-75880898 MS.gene69889:CDS
GGATATGTGGTGGGATACAA+AGG 0.546750 3.4:-75879205 MS.gene69889:CDS
TTCCAAGCTGGCAACCAGAA+CGG 0.550031 3.4:-75879144 MS.gene69889:CDS
AGCTCCCTATAAACGCAGCT+TGG 0.550821 3.4:-75879484 MS.gene69889:CDS
ATCTCTCCAGCCAGTTGATG+AGG 0.560761 3.4:+75880799 None:intergenic
AACACTGTTATTAACCTCAA+AGG 0.561939 3.4:-75880669 MS.gene69889:CDS
CAGGACATCTTGTTCCAAGC+TGG 0.567894 3.4:-75879156 MS.gene69889:CDS
CTAGAGTCTCCAGCAGGAGT+GGG 0.569156 3.4:-75880897 MS.gene69889:CDS
TACCCCAGTGAGGAACAGCC+AGG 0.578470 3.4:+75881114 None:intergenic
CCCTTCAGAATCAACTCTGA+TGG 0.578815 3.4:-75881069 MS.gene69889:CDS
ATAACCGGAGAGAGTTATGC+TGG 0.580139 3.4:-75880747 MS.gene69889:CDS
CTCTTCCTAGAGTCTCCAGC+AGG 0.582921 3.4:-75880903 MS.gene69889:CDS
TCGATGAGATACTTGACAGT+TGG 0.583309 3.4:+75879747 None:intergenic
TAATAATAATGACAGATTGG+AGG 0.585531 3.4:-75879226 MS.gene69889:intron
TATGGAGCCTTTCAGGAGCT+AGG 0.588840 3.4:-75881093 MS.gene69889:CDS
GAAGGGTCCTAGCTCCTGAA+AGG 0.589336 3.4:+75881086 None:intergenic
AGGAGATAAATCAACTGCCA+AGG 0.589939 3.4:-75880835 MS.gene69889:CDS
GCTTGCATTAGCCCCTCTTG+AGG 0.590255 3.4:+75881803 None:intergenic
CCAGTTGATGAGGAAGACAT+AGG 0.602150 3.4:+75880809 None:intergenic
TTAGTATTGTGGCTTAATGG+AGG 0.610025 3.4:-75881638 MS.gene69889:intron
TAGGAGGCAAACTTCCATAG+AGG 0.611627 3.4:+75879098 None:intergenic
CAGTGGTGATACAGATGCAG+TGG 0.613592 3.4:-75879546 MS.gene69889:CDS
ATGACAGATTGGAGGATATG+TGG 0.614174 3.4:-75879218 MS.gene69889:intron
GAACCATAATAATCAGAACA+AGG 0.615337 3.4:+75880274 None:intergenic
AGTTCAAAGCATGAGTCCAA+AGG 0.615853 3.4:+75880494 None:intergenic
CTACGTCACTGTTGATCCTG+AGG 0.618660 3.4:-75881720 MS.gene69889:CDS
TAATTTGATGGTGAACTAGG+AGG 0.626163 3.4:+75879082 None:intergenic
ATGATGCCACAAGTTTAAAG+GGG 0.629930 3.4:-75880528 MS.gene69889:CDS
GTCAAGTATCTCATCGATAG+CGG 0.632646 3.4:-75879741 MS.gene69889:CDS
CAGATTGGAGGATATGTGGT+GGG 0.633074 3.4:-75879214 MS.gene69889:CDS
AACCATAATAATCAGAACAA+GGG 0.642040 3.4:+75880275 None:intergenic
CTGATGTTGCACCTCAAGAG+GGG 0.658453 3.4:-75881814 MS.gene69889:CDS
TCCCAAGAAAGAGTCTTGGG+AGG 0.663424 3.4:+75881875 None:intergenic
AGAATAGTGGATGCAAGCTG+AGG 0.669219 3.4:+75880714 None:intergenic
TGCCTATCTAAATAGACCAG+AGG 0.674801 3.4:-75880251 MS.gene69889:CDS
AGAGAGGAGTCATGACACAA+TGG 0.676190 3.4:+75880337 None:intergenic
ACAGATTGGAGGATATGTGG+TGG 0.699486 3.4:-75879215 MS.gene69889:intron
AAAGGCTCCATACCCCAGTG+AGG 0.728637 3.4:+75881104 None:intergenic
GACCTGGCTGTTCCTCACTG+GGG 0.729552 3.4:-75881116 MS.gene69889:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAATTAAAGTTGTAT+TGG + chr3.4:75881016-75881035 None:intergenic 10.0%
!! ATTTCAAAAATGTTATTACT+TGG + chr3.4:75881824-75881843 None:intergenic 15.0%
!! CATATTTCATAATGATAAAT+TGG + chr3.4:75880145-75880164 None:intergenic 15.0%
!!! ACTTTAAAAAATTTCAGATA+GGG - chr3.4:75880518-75880537 MS.gene69889:CDS 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG + chr3.4:75880976-75880995 None:intergenic 15.0%
!!! TACTTTAAAAAATTTCAGAT+AGG - chr3.4:75880517-75880536 MS.gene69889:CDS 15.0%
!!! TTGTTATTTTGTAAATTTGA+GGG - chr3.4:75881719-75881738 MS.gene69889:CDS 15.0%
!!! TTTGTTATTTTGTAAATTTG+AGG - chr3.4:75881718-75881737 MS.gene69889:CDS 15.0%
!! AAATGGAATGAAATAATCAT+TGG + chr3.4:75881142-75881161 None:intergenic 20.0%
!! TATCAATAATGTAACACTAA+AGG - chr3.4:75880678-75880697 MS.gene69889:CDS 20.0%
!! TTAATTTATTCGTAGTTTGA+TGG - chr3.4:75881654-75881673 MS.gene69889:CDS 20.0%
!! TTATCACAATTTAAATCTTC+AGG - chr3.4:75881424-75881443 MS.gene69889:intron 20.0%
!! TTGTAATAATAATGACAGAT+TGG - chr3.4:75881853-75881872 MS.gene69889:CDS 20.0%
!!! CTTTTGATATTAAAAGAACA+TGG - chr3.4:75879747-75879766 MS.gene69889:CDS 20.0%
!!! TGTCTTTTTCTAATAAAGTA+AGG - chr3.4:75881093-75881112 MS.gene69889:CDS 20.0%
!!! TTGCAAATTTTCTTATTTAG+AGG + chr3.4:75879607-75879626 None:intergenic 20.0%
! AACCATAATAATCAGAACAA+GGG + chr3.4:75880810-75880829 None:intergenic 25.0%
! AATACTAAAGGTTTAGTTGA+AGG + chr3.4:75879432-75879451 None:intergenic 25.0%
! ATACTAAAGGTTTAGTTGAA+GGG + chr3.4:75879431-75879450 None:intergenic 25.0%
! ATTACTCAACTTGAGTTTAT+TGG - chr3.4:75879110-75879129 MS.gene69889:CDS 25.0%
! ATTACTTGGTATACAAGAAA+AGG + chr3.4:75881810-75881829 None:intergenic 25.0%
! GCATTAAATTATGGATATAC+AGG - chr3.4:75881363-75881382 MS.gene69889:intron 25.0%
! TAATAATAATGACAGATTGG+AGG - chr3.4:75881856-75881875 MS.gene69889:CDS 25.0%
! TACTCTTGATGAAATTATTC+CGG - chr3.4:75879807-75879826 MS.gene69889:intron 25.0%
! TATAGAATATTACCTGCAAT+GGG + chr3.4:75880893-75880912 None:intergenic 25.0%
! TTATAGAATATTACCTGCAA+TGG + chr3.4:75880894-75880913 None:intergenic 25.0%
! TTTATTGTTGTGAAGAATAG+TGG + chr3.4:75880384-75880403 None:intergenic 25.0%
!!! AGAGCTTTTCTATTATTTTG+TGG - chr3.4:75879392-75879411 MS.gene69889:intron 25.0%
AAAGTTTGAAAGGGTAAAGT+TGG + chr3.4:75881040-75881059 None:intergenic 30.0%
AACACTGTTATTAACCTCAA+AGG - chr3.4:75880413-75880432 MS.gene69889:CDS 30.0%
ATTCTAATTCACTCCATATG+TGG - chr3.4:75881773-75881792 MS.gene69889:CDS 30.0%
CAACTAAACCTTTAGTATTG+TGG - chr3.4:75879433-75879452 MS.gene69889:intron 30.0%
GAACCATAATAATCAGAACA+AGG + chr3.4:75880811-75880830 None:intergenic 30.0%
GGTAATAAATCAACCACATA+TGG + chr3.4:75881789-75881808 None:intergenic 30.0%
TAAAAGAACATGGTAAAACC+TGG - chr3.4:75879757-75879776 MS.gene69889:CDS 30.0%
TATCTTTCCATGTTCAAAGA+AGG + chr3.4:75880704-75880723 None:intergenic 30.0%
TGTGAATAATGACTAATGAG+AGG - chr3.4:75881478-75881497 MS.gene69889:intron 30.0%
TGTGAGAGAAAAGTTTGAAA+GGG + chr3.4:75881049-75881068 None:intergenic 30.0%
TGTGAGTTACATTTCATACT+TGG + chr3.4:75880467-75880486 None:intergenic 30.0%
TTCATGCAAAACCTACAAAT+TGG - chr3.4:75880866-75880885 MS.gene69889:CDS 30.0%
TTCTATGCTAATCAAGAATG+TGG - chr3.4:75881247-75881266 MS.gene69889:intron 30.0%
! ACATAATGTTTGAAACTCAC+AGG - chr3.4:75879944-75879963 MS.gene69889:intron 30.0%
! ACCAGAGATTTTTACATAAC+CGG - chr3.4:75880320-75880339 MS.gene69889:CDS 30.0%
! GTGAATTTTGAGCAATATTC+AGG - chr3.4:75879339-75879358 MS.gene69889:intron 30.0%
! TCATAGTTGTAAGAGTGTAA+GGG + chr3.4:75879859-75879878 None:intergenic 30.0%
! TTCCAACTCCTAAATTTTGA+TGG - chr3.4:75881501-75881520 MS.gene69889:intron 30.0%
!! AAGTGACACTCATTTTGAAA+CGG + chr3.4:75880950-75880969 None:intergenic 30.0%
AAATAGACCAGAGGTTCAAA+AGG - chr3.4:75880840-75880859 MS.gene69889:CDS 35.0%
AACATACAGAAATGCCTTTG+AGG + chr3.4:75880430-75880449 None:intergenic 35.0%
AACCTCTGGTCTATTTAGAT+AGG + chr3.4:75880836-75880855 None:intergenic 35.0%
AACCTGTATTCTTGGAATGA+AGG - chr3.4:75880052-75880071 MS.gene69889:intron 35.0%
AATTTCAGATAGGGAATGCT+TGG - chr3.4:75880527-75880546 MS.gene69889:CDS 35.0%
AGAACGCACCATCAAAATTT+AGG + chr3.4:75881512-75881531 None:intergenic 35.0%
ATGATCTCATCATTCCTCTA+TGG - chr3.4:75881970-75881989 MS.gene69889:CDS 35.0%
ATGATGCCACAAGTTTAAAG+GGG - chr3.4:75880554-75880573 MS.gene69889:intron 35.0%
CACCATCAAAATTTAGGAGT+TGG + chr3.4:75881506-75881525 None:intergenic 35.0%
CATTCCAAGAATACAGGTTA+CGG + chr3.4:75880051-75880070 None:intergenic 35.0%
CCATTAAGCCACAATACTAA+AGG + chr3.4:75879444-75879463 None:intergenic 35.0%
CCTTTAGTATTGTGGCTTAA+TGG - chr3.4:75879441-75879460 MS.gene69889:intron 35.0%
GAAAAACCCCTTTAAACTTG+TGG + chr3.4:75880563-75880582 None:intergenic 35.0%
GACCCTTGTTCTGATTATTA+TGG - chr3.4:75880805-75880824 MS.gene69889:CDS 35.0%
GATGATGCCACAAGTTTAAA+GGG - chr3.4:75880553-75880572 MS.gene69889:intron 35.0%
GGTTTAGTTGAAGGGTTATA+TGG + chr3.4:75879423-75879442 None:intergenic 35.0%
GTGTGAGAGAAAAGTTTGAA+AGG + chr3.4:75881050-75881069 None:intergenic 35.0%
TATACCGTAACCTGTATTCT+TGG - chr3.4:75880044-75880063 MS.gene69889:intron 35.0%
TCCGGTTATGTAAAAATCTC+TGG + chr3.4:75880324-75880343 None:intergenic 35.0%
TGCTCAAAATTCACTCCATA+GGG + chr3.4:75879333-75879352 None:intergenic 35.0%
TTAGTATTGTGGCTTAATGG+AGG - chr3.4:75879444-75879463 MS.gene69889:intron 35.0%
TTGCTCAAAATTCACTCCAT+AGG + chr3.4:75879334-75879353 None:intergenic 35.0%
TTGGCACACAGATTAAGAAA+TGG + chr3.4:75881159-75881178 None:intergenic 35.0%
! ATCTCCTGATTTGTCATAGT+CGG + chr3.4:75880234-75880253 None:intergenic 35.0%
! CTCATAGTTGTAAGAGTGTA+AGG + chr3.4:75879860-75879879 None:intergenic 35.0%
! TCGATAGCGGCATTAAATTA+TGG - chr3.4:75881354-75881373 MS.gene69889:intron 35.0%
! TCTCTGAAAAATGGTTCAAC+TGG - chr3.4:75880763-75880782 MS.gene69889:CDS 35.0%
! TGATGATGCCACAAGTTTAA+AGG - chr3.4:75880552-75880571 MS.gene69889:intron 35.0%
! TGATGATGCTCATAATAACC+AGG + chr3.4:75879778-75879797 None:intergenic 35.0%
! TTGACATTTGTTACAGTGAG+AGG - chr3.4:75881901-75881920 MS.gene69889:CDS 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr3.4:75881185-75881204 MS.gene69889:intron 35.0%
!! ACAGTTGGTAAGATGGTAAT+TGG + chr3.4:75881323-75881342 None:intergenic 35.0%
!! AGTTGAACCATTTTTCAGAG+AGG + chr3.4:75880764-75880783 None:intergenic 35.0%
!! ATACTTGACAGTTGGTAAGA+TGG + chr3.4:75881330-75881349 None:intergenic 35.0%
!! TAATTTGATGGTGAACTAGG+AGG + chr3.4:75882003-75882022 None:intergenic 35.0%
!! TTCAAAATGAGTGTCGCTTT+AGG + chr3.4:75881184-75881203 None:intergenic 35.0%
!!! AAATCTCTGGTTTTGTACTG+TGG + chr3.4:75880311-75880330 None:intergenic 35.0%
ACATCCGACTATGACAAATC+AGG - chr3.4:75880227-75880246 MS.gene69889:CDS 40.0%
ACTTCCCAAGAAAGAGTCTT+GGG + chr3.4:75879213-75879232 None:intergenic 40.0%
AGGAGATAAATCAACTGCCA+AGG - chr3.4:75880247-75880266 MS.gene69889:CDS 40.0%
AGTTCAAAGCATGAGTCCAA+AGG + chr3.4:75880591-75880610 None:intergenic 40.0%
ATAGTCGTCTTCTTACTGAC+TGG - chr3.4:75881288-75881307 MS.gene69889:intron 40.0%
ATGACAGATTGGAGGATATG+TGG - chr3.4:75881864-75881883 MS.gene69889:CDS 40.0%
CACCTTCATTCCAAGAATAC+AGG + chr3.4:75880057-75880076 None:intergenic 40.0%
CTAAAGGCCTTCTTTGAACA+TGG - chr3.4:75880694-75880713 MS.gene69889:CDS 40.0%
CTTATTTAGAGGTCTCTGCA+AGG + chr3.4:75879596-75879615 None:intergenic 40.0%
GTCAAGTATCTCATCGATAG+CGG - chr3.4:75881341-75881360 MS.gene69889:intron 40.0%
TCGATGAGATACTTGACAGT+TGG + chr3.4:75881338-75881357 None:intergenic 40.0%
TGATGAAATTATTCCGGCCA+TGG - chr3.4:75879813-75879832 MS.gene69889:intron 40.0%
TGCCTATCTAAATAGACCAG+AGG - chr3.4:75880831-75880850 MS.gene69889:CDS 40.0%
TGCGTTTATAGGGAGCTTAA+GGG + chr3.4:75881595-75881614 None:intergenic 40.0%
! ACCTCTTTTCCAGAATACCA+TGG + chr3.4:75881627-75881646 None:intergenic 40.0%
! CATGACTCCTCTCTGAAAAA+TGG - chr3.4:75880754-75880773 MS.gene69889:CDS 40.0%
! CCATCAGAGTTGATTCTGAA+GGG + chr3.4:75880016-75880035 None:intergenic 40.0%
! TCCATGGTATTCTGGAAAAG+AGG - chr3.4:75881623-75881642 MS.gene69889:intron 40.0%
!! AGGGGTTTTTCGATTACCTT+TGG - chr3.4:75880572-75880591 MS.gene69889:intron 40.0%
!! GCTTGACTAGCTTTGCTATA+TGG + chr3.4:75879144-75879163 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr3.4:75881189-75881208 MS.gene69889:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr3.4:75881208-75881189 None:intergenic 40.0%
!!! TGACACTCATTTTGAAACGG+AGG + chr3.4:75880947-75880966 None:intergenic 40.0%
ACAGATTGGAGGATATGTGG+TGG - chr3.4:75881867-75881886 MS.gene69889:CDS 45.0%
AGAGAGGAGTCATGACACAA+TGG + chr3.4:75880748-75880767 None:intergenic 45.0%
ATAACCGGAGAGAGTTATGC+TGG - chr3.4:75880335-75880354 MS.gene69889:CDS 45.0%
ATCATTGTCAAAGCCCGTTC+TGG + chr3.4:75881955-75881974 None:intergenic 45.0%
ATCCTCCCAAGACTCTTTCT+TGG - chr3.4:75879205-75879224 MS.gene69889:CDS 45.0%
ATGACCAGCATAACTCTCTC+CGG + chr3.4:75880342-75880361 None:intergenic 45.0%
CAAGACTCTTTCTTGGGAAG+TGG - chr3.4:75879212-75879231 MS.gene69889:CDS 45.0%
CACTTCCCAAGAAAGAGTCT+TGG + chr3.4:75879214-75879233 None:intergenic 45.0%
CAGATTGGAGGATATGTGGT+GGG - chr3.4:75881868-75881887 MS.gene69889:CDS 45.0%
CCCTTCAGAATCAACTCTGA+TGG - chr3.4:75880013-75880032 MS.gene69889:intron 45.0%
CCTATGTCTTCCTCATCAAC+TGG - chr3.4:75880273-75880292 MS.gene69889:CDS 45.0%
CTACAAATTGGACCCATTGC+AGG - chr3.4:75880878-75880897 MS.gene69889:CDS 45.0%
CTGCAATGGGTCCAATTTGT+AGG + chr3.4:75880880-75880899 None:intergenic 45.0%
CTGCGTTTATAGGGAGCTTA+AGG + chr3.4:75881596-75881615 None:intergenic 45.0%
GACAAGATTGTCACATTGCC+TGG - chr3.4:75879303-75879322 MS.gene69889:intron 45.0%
GAGAGTTTAACAACCATGGC+CGG + chr3.4:75879829-75879848 None:intergenic 45.0%
GCCATGGTTGTTAAACTCTC+GGG - chr3.4:75879829-75879848 MS.gene69889:intron 45.0%
GGCCATGGTTGTTAAACTCT+CGG - chr3.4:75879828-75879847 MS.gene69889:intron 45.0%
TAGGAGGCAAACTTCCATAG+AGG + chr3.4:75881987-75882006 None:intergenic 45.0%
TATGAAAATCCCACTCCTGC+TGG + chr3.4:75880197-75880216 None:intergenic 45.0%
TCCTCCCAAGACTCTTTCTT+GGG - chr3.4:75879206-75879225 MS.gene69889:CDS 45.0%
TGCATCTGTATCACCACTGT+AGG + chr3.4:75881535-75881554 None:intergenic 45.0%
TTTGTTACAGTGAGAGGAGC+AGG - chr3.4:75881907-75881926 MS.gene69889:CDS 45.0%
! GCCATCAGAGTTGATTCTGA+AGG + chr3.4:75880017-75880036 None:intergenic 45.0%
! GGATATGTGGTGGGATACAA+AGG - chr3.4:75881877-75881896 MS.gene69889:CDS 45.0%
! TGAAGAGCCTTTTGAACCTC+TGG + chr3.4:75880850-75880869 None:intergenic 45.0%
! TGTTTGAAACTCACAGGACC+TGG - chr3.4:75879950-75879969 MS.gene69889:intron 45.0%
!! AGAATAGTGGATGCAAGCTG+AGG + chr3.4:75880371-75880390 None:intergenic 45.0%
!! ATGAGTGTCGCTTTAGGCTT+TGG + chr3.4:75881178-75881197 None:intergenic 45.0%
!! CCAGTTGATGAGGAAGACAT+AGG + chr3.4:75880276-75880295 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr3.4:75881188-75881207 MS.gene69889:intron 45.0%
AAAAGCTCTCTTCCAGCCTC+AGG + chr3.4:75879381-75879400 None:intergenic 50.0%
ACATTGCCTGGTCAACCCTA+TGG - chr3.4:75879315-75879334 MS.gene69889:intron 50.0%
AGCTCCCTATAAACGCAGCT+TGG - chr3.4:75881598-75881617 MS.gene69889:intron 50.0%
CAGGACATCTTGTTCCAAGC+TGG - chr3.4:75881926-75881945 MS.gene69889:CDS 50.0%
CAGTGGTGATACAGATGCAG+TGG - chr3.4:75881536-75881555 MS.gene69889:intron 50.0%
CTACGTCACTGTTGATCCTG+AGG - chr3.4:75879362-75879381 MS.gene69889:intron 50.0%
CTGATGTTGCACCTCAAGAG+GGG - chr3.4:75879268-75879287 MS.gene69889:intron 50.0%
GACGCCAAGCTGCGTTTATA+GGG + chr3.4:75881605-75881624 None:intergenic 50.0%
GAGGAAGACATAGGCATCCT+TGG + chr3.4:75880267-75880286 None:intergenic 50.0%
GCCCGAGAGTTTAACAACCA+TGG + chr3.4:75879833-75879852 None:intergenic 50.0%
GCTGATGTTGCACCTCAAGA+GGG - chr3.4:75879267-75879286 MS.gene69889:intron 50.0%
TATGGAGCCTTTCAGGAGCT+AGG - chr3.4:75879989-75880008 MS.gene69889:intron 50.0%
TCCCAAGAAAGAGTCTTGGG+AGG + chr3.4:75879210-75879229 None:intergenic 50.0%
TGCTGATGTTGCACCTCAAG+AGG - chr3.4:75879266-75879285 MS.gene69889:intron 50.0%
TGTCTTCCTCATCAACTGGC+TGG - chr3.4:75880277-75880296 MS.gene69889:CDS 50.0%
TTCACTCCATAGGGTTGACC+AGG + chr3.4:75879324-75879343 None:intergenic 50.0%
TTCCAAGCTGGCAACCAGAA+CGG - chr3.4:75881938-75881957 MS.gene69889:CDS 50.0%
! ATCTCTCCAGCCAGTTGATG+AGG + chr3.4:75880286-75880305 None:intergenic 50.0%
AAAGGCTCCATACCCCAGTG+AGG + chr3.4:75879981-75880000 None:intergenic 55.0%
ACTGGGGTATGGAGCCTTTC+AGG - chr3.4:75879982-75880001 MS.gene69889:intron 55.0%
CTAGAGTCTCCAGCAGGAGT+GGG - chr3.4:75880185-75880204 MS.gene69889:intron 55.0%
CTCTTCCTAGAGTCTCCAGC+AGG - chr3.4:75880179-75880198 MS.gene69889:intron 55.0%
GAAGGGTCCTAGCTCCTGAA+AGG + chr3.4:75879999-75880018 None:intergenic 55.0%
GATGGTGCGTTCTCCTACAG+TGG - chr3.4:75881519-75881538 MS.gene69889:intron 55.0%
GCTTGCATTAGCCCCTCTTG+AGG + chr3.4:75879282-75879301 None:intergenic 55.0%
GCTTGGCGTCCATGGTATTC+TGG - chr3.4:75881615-75881634 MS.gene69889:intron 55.0%
GGACGCCAAGCTGCGTTTAT+AGG + chr3.4:75881606-75881625 None:intergenic 55.0%
GTCACTGTTGATCCTGAGGC+TGG - chr3.4:75879366-75879385 MS.gene69889:intron 55.0%
TAAACGCAGCTTGGCGTCCA+TGG - chr3.4:75881607-75881626 MS.gene69889:intron 55.0%
TCCAAGCTGGCAACCAGAAC+GGG - chr3.4:75881939-75881958 MS.gene69889:CDS 55.0%
AGGACCTGGCTGTTCCTCAC+TGG - chr3.4:75879964-75879983 MS.gene69889:intron 60.0%
CCACTCCTGCTGGAGACTCT+AGG + chr3.4:75880187-75880206 None:intergenic 60.0%
CCTAGAGTCTCCAGCAGGAG+TGG - chr3.4:75880184-75880203 MS.gene69889:intron 60.0%
GACCTGGCTGTTCCTCACTG+GGG - chr3.4:75879966-75879985 MS.gene69889:intron 60.0%
GGACCTGGCTGTTCCTCACT+GGG - chr3.4:75879965-75879984 MS.gene69889:intron 60.0%
GGCTGTTCCTCACTGGGGTA+TGG - chr3.4:75879971-75879990 MS.gene69889:intron 60.0%
TACCCCAGTGAGGAACAGCC+AGG + chr3.4:75879971-75879990 None:intergenic 60.0%
! GCCCGTTCTGGTTGCCAGCT+TGG + chr3.4:75881943-75881962 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 75879081 75882023 75879081 ID=MS.gene69889
chr3.4 mRNA 75879081 75882023 75879081 ID=MS.gene69889.t1;Parent=MS.gene69889
chr3.4 exon 75881639 75882023 75881639 ID=MS.gene69889.t1.exon1;Parent=MS.gene69889.t1
chr3.4 CDS 75881639 75882023 75881639 ID=cds.MS.gene69889.t1;Parent=MS.gene69889.t1
chr3.4 exon 75881031 75881138 75881031 ID=MS.gene69889.t1.exon2;Parent=MS.gene69889.t1
chr3.4 CDS 75881031 75881138 75881031 ID=cds.MS.gene69889.t1;Parent=MS.gene69889.t1
chr3.4 exon 75880662 75880936 75880662 ID=MS.gene69889.t1.exon3;Parent=MS.gene69889.t1
chr3.4 CDS 75880662 75880936 75880662 ID=cds.MS.gene69889.t1;Parent=MS.gene69889.t1
chr3.4 exon 75880205 75880569 75880205 ID=MS.gene69889.t1.exon4;Parent=MS.gene69889.t1
chr3.4 CDS 75880205 75880569 75880205 ID=cds.MS.gene69889.t1;Parent=MS.gene69889.t1
chr3.4 exon 75879720 75879812 75879720 ID=MS.gene69889.t1.exon5;Parent=MS.gene69889.t1
chr3.4 CDS 75879720 75879812 75879720 ID=cds.MS.gene69889.t1;Parent=MS.gene69889.t1
chr3.4 exon 75879460 75879565 75879460 ID=MS.gene69889.t1.exon6;Parent=MS.gene69889.t1
chr3.4 CDS 75879460 75879565 75879460 ID=cds.MS.gene69889.t1;Parent=MS.gene69889.t1
chr3.4 exon 75879081 75879233 75879081 ID=MS.gene69889.t1.exon7;Parent=MS.gene69889.t1
chr3.4 CDS 75879081 75879233 75879081 ID=cds.MS.gene69889.t1;Parent=MS.gene69889.t1
Gene Sequence

>MS.gene69889

ATGAAGAAGGTTTCTCTTTATGCTTGTTTATTACTCAACTTGAGTTTATTGGTTATTTTTCCATATAGCAAAGCTAGTCAAGCTGATAAACTCAATGAGTTTATTCTGTCTAGAAAATCTCAGAATCCTCCCAAGACTCTTTCTTGGGAAGTGGAAGATGCAGTGAAAACACTTTCTTCTTCTGCTGCTGATGTTGCACCTCAAGAGGGGCTAATGCAAGCTGACAAGATTGTCACATTGCCTGGTCAACCCTATGGAGTGAATTTTGAGCAATATTCAGGCTACGTCACTGTTGATCCTGAGGCTGGAAGAGAGCTTTTCTATTATTTTGTGGAGTCTCCATATAACCCTTCAACTAAACCTTTAGTATTGTGGCTTAATGGAGGTAAAAATTGCATTAGAAACTATTTTTCACAGTTAACTTTTGATCTTTTGTATGCATTTTCTATTTTTTATGTTCTTTAATGTGTCATTGTCTATTTATATTATGATGTTTGAAATTGTGAAAATTACCTTGCAGAGACCTCTAAATAAGAAAATTTGCAACACTACACAAGATAAAAATAGTTTATAAATAGATTATTAATTATACTTGATTGATGATATAACTTCATTTTCATAGTAGTGAAACATGATAGTATAGTTTTAATATTGAACTTTATTTTTCTTTTGATATTAAAAGAACATGGTAAAACCTGGTTATTATGAGCATCATCATCTGAATTTTACTCTTGATGAAATTATTCCGGCCATGGTTGTTAAACTCTCGGGCTAACCCTTACACTCTTACAACTATGAGTCGAGAGTCTATGTAAGCTCAACAAGTTTACTTAGTCGAGATTATGACAGCTTTGATTCTGACAACATAATGTTTGAAACTCACAGGACCTGGCTGTTCCTCACTGGGGTATGGAGCCTTTCAGGAGCTAGGACCCTTCAGAATCAACTCTGATGGCAAAACACTATACCGTAACCTGTATTCTTGGAATGAAGGTGAGACAATTATCAATAGAACGAAATTGTTTCTTATGATGCATTTATTCTGAGACATGAATATTTTACCAATTTATCATTATGAAATATGCAGTTGCAAATATACTCTTCCTAGAGTCTCCAGCAGGAGTGGGATTTTCATACTCAAATACAACATCCGACTATGACAAATCAGGAGATAAATCAACTGCCAAGGATGCCTATGTCTTCCTCATCAACTGGCTGGAGAGATTTCCACAGTACAAAACCAGAGATTTTTACATAACCGGAGAGAGTTATGCTGGTCATTATGTTCCTCAGCTTGCATCCACTATTCTTCACAACAATAAACTCTATAACAACACTGTTATTAACCTCAAAGGCATTTCTGTATGTTCAAACTTCAAAAGACCAAGTATGAAATGTAACTCACAATCTCATAAATCTAACTTCATTATTATCTTTACTTTAAAAAATTTCAGATAGGGAATGCTTGGATTGATGATGCCACAAGTTTAAAGGGGTTTTTCGATTACCTTTGGACTCATGCTTTGAACTCAGATCAAACTCATGAGTTGATTGAGAAGTACTGTGACTTCACTACTGAAAATGTTTCAGCAATTTGTATCAATAATGTAACACTAAAGGCCTTCTTTGAACATGGAAAGATAGACTTATATAACATCTATGCTCCATTGTGTCATGACTCCTCTCTGAAAAATGGTTCAACTGGTTATGTAAGTAATGATTTTGACCCTTGTTCTGATTATTATGGTTCTGCCTATCTAAATAGACCAGAGGTTCAAAAGGCTCTTCATGCAAAACCTACAAATTGGACCCATTGCAGGTAATATTCTATAATCACTTTTAAGAAATTCTAAAATAGTACTCCCTCCGTTTCAAAATGAGTGTCACTTTAGCCAAAAAAATTTGTTTCAAAATGAATGTCGCTTTACATTTCCAATACAACTTTAATTTTTTTTTCCAACTTTACCCTTTCAAACTTTTCTCTCACACAATTTTCAATGCAACTTTAAGTTTGTCTTTTTCTAATAAAGTAAGGACAGTTTAGTAAAAGCGTTATATCCAATGATTATTTCATTCCATTTCTTAATCTGTGTGCCAAAGCCTAAAGCGACACTCATTTTGAAACGGAGGGAGTACTATTTTTTTGACACTTACATAAATTTATTTTTCTATGCTAATCAAGAATGTGGTATTTTTTATGACAATTCATAGTCGTCTTCTTACTGACTGGAAAGACAGTCCAATTACCATCTTACCAACTGTCAAGTATCTCATCGATAGCGGCATTAAATTATGGATATACAGGCAAGTTCACTTTCATTATAATATAAATTCTTAACACTGTTATCACAATTTAAATCTTCAGGAACAATATTATAATTATAACATGTAGACTATTGTGAATAATGACTAATGAGAGGTTCCAACTCCTAAATTTTGATGGTGCGTTCTCCTACAGTGGTGATACAGATGCAGTGGTTTCAGTTACATCTTCAAGATATTCAATCAACACCCTTAAGCTCCCTATAAACGCAGCTTGGCGTCCATGGTATTCTGGAAAAGAGGTACTGAAATTAATTTATTCGTAGTTTGATGGAGAAACTAAACTAATTGACATTCTATAATTTAAGAGACTGATTTGTTATTTTGTAAATTTGAGGGACAATTGACACTTACAATGTCGAGTACAAAAATTCTAATTCACTCCATATGTGGTTGATTTATTACCTTTTCTTGTATACCAAGTAATAACATTTTTGAAATGTGATCAAATTGTAATAATAATGACAGATTGGAGGATATGTGGTGGGATACAAAGGATTGACATTTGTTACAGTGAGAGGAGCAGGACATCTTGTTCCAAGCTGGCAACCAGAACGGGCTTTGACAATGATCTCATCATTCCTCTATGGAAGTTTGCCTCCTAGTTCACCATCAAATTAA
Protein sequence

>MS.gene69889.t1

MKKVSLYACLLLNLSLLVIFPYSKASQADKLNEFILSRKSQNPPKTLSWEVEDAVKTLSSSAADVAPQEGLMQADKIVTLPGQPYGVNFEQYSGYVTVDPEAGRELFYYFVESPYNPSTKPLVLWLNGGPGCSSLGYGAFQELGPFRINSDGKTLYRNLYSWNEVANILFLESPAGVGFSYSNTTSDYDKSGDKSTAKDAYVFLINWLERFPQYKTRDFYITGESYAGHYVPQLASTILHNNKLYNNTVINLKGISIGNAWIDDATSLKGFFDYLWTHALNSDQTHELIEKYCDFTTENVSAICINNVTLKAFFEHGKIDLYNIYAPLCHDSSLKNGSTGYVSNDFDPCSDYYGSAYLNRPEVQKALHAKPTNWTHCSRLLTDWKDSPITILPTVKYLIDSGIKLWIYSGDTDAVVSVTSSRYSINTLKLPINAAWRPWYSGKEIGGYVVGYKGLTFVTVRGAGHLVPSWQPERALTMISSFLYGSLPPSSPSN