Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70395.t1 | XP_003593138.1 | 100 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 6.00E-53 | 216.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70395.t1 | Q96499 | 97.1 | 105 | 3 | 0 | 1 | 105 | 1 | 105 | 2.1e-54 | 212.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70395.t1 | A0A2K3MQ78 | 100.0 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 4.3e-53 | 216.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055455 | MS.gene70395 | 0.814827 | 1.26E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70395 | MS.gene35046 | PPI |
| MS.gene70395 | MS.gene01133 | PPI |
| MS.gene70395 | MS.gene071958 | PPI |
| MS.gene002165 | MS.gene70395 | PPI |
| MS.gene70395 | MS.gene028410 | PPI |
| MS.gene70395 | MS.gene051330 | PPI |
| MS.gene047093 | MS.gene70395 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70395.t1 | MTR_7g100280 | 100.000 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.57e-71 | 208 |
| MS.gene70395.t1 | MTR_4g094308 | 100.000 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.57e-71 | 208 |
| MS.gene70395.t1 | MTR_2g008180 | 100.000 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.57e-71 | 208 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70395.t1 | AT4G14320 | 92.381 | 105 | 8 | 0 | 1 | 105 | 1 | 105 | 1.00e-66 | 196 |
| MS.gene70395.t1 | AT3G23390 | 92.381 | 105 | 8 | 0 | 1 | 105 | 1 | 105 | 1.00e-66 | 196 |
| MS.gene70395.t1 | AT4G14320 | 91.398 | 93 | 8 | 0 | 1 | 93 | 1 | 93 | 3.30e-57 | 174 |
Find 18 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGTTACCCAATACAAAA+AGG | 0.271604 | 4.3:-20016401 | MS.gene70395:CDS |
| TGTTTCACAACACGCAATTA+AGG | 0.365888 | 4.3:-20016130 | MS.gene70395:intron |
| CAAACCCGTCTTTCACAAAA+AGG | 0.369511 | 4.3:-20016308 | MS.gene70395:intron |
| TAAAGATTCCATCGCTGCTC+AGG | 0.399194 | 4.3:-20016377 | MS.gene70395:CDS |
| CGTTATGACCGCAAACAATC+TGG | 0.415551 | 4.3:-20016346 | MS.gene70395:CDS |
| GTATTGGGTAACTTTGTGTA+AGG | 0.453756 | 4.3:+20016408 | None:intergenic |
| CACCATAACCAGATTGTTTG+CGG | 0.454458 | 4.3:+20016338 | None:intergenic |
| GACCGCAAACAATCTGGTTA+TGG | 0.454865 | 4.3:-20016340 | MS.gene70395:CDS |
| AGGTGCAAGCACTTTGAGAT+TGG | 0.462716 | 4.3:-20016016 | MS.gene70395:CDS |
| TGAGATTGGTGGTGACAAGA+AGG | 0.508837 | 4.3:-20016002 | MS.gene70395:CDS |
| CGCAAACAATCTGGTTATGG+TGG | 0.541862 | 4.3:-20016337 | MS.gene70395:CDS |
| GGTGGTGACAAGAAGGGAAA+AGG | 0.543946 | 4.3:-20015995 | MS.gene70395:CDS |
| TGCAAGCACTTTGAGATTGG+TGG | 0.545430 | 4.3:-20016013 | MS.gene70395:CDS |
| GAGATTGGTGGTGACAAGAA+GGG | 0.555011 | 4.3:-20016001 | MS.gene70395:CDS |
| CTACTAAGAAAATTGTGTTG+AGG | 0.599248 | 4.3:-20016176 | MS.gene70395:CDS |
| AAAGATTCCATCGCTGCTCA+GGG | 0.607025 | 4.3:-20016376 | MS.gene70395:CDS |
| GAAAACCTACTGCAAAAGCA+AGG | 0.613372 | 4.3:-20016446 | MS.gene70395:CDS |
| GTGTTGAGGCTTCAGTGTCA+AGG | 0.618711 | 4.3:-20016162 | MS.gene70395:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTCAATTTTTGTTGTTAT+AGG | - | chr4.3:20016072-20016091 | MS.gene70395:intron | 15.0% |
| !! | TCAGATTAAGCAAATATTTA+AGG | + | chr4.3:20016293-20016312 | None:intergenic | 20.0% |
| !!! | AGTGAAATTTTTACTGTTTT+TGG | - | chr4.3:20016482-20016501 | MS.gene70395:intron | 20.0% |
| !!! | GTGAAATTTTTACTGTTTTT+GGG | - | chr4.3:20016483-20016502 | MS.gene70395:intron | 20.0% |
| ! | AAAGTTACCCAATACAAAAA+GGG | - | chr4.3:20016160-20016179 | MS.gene70395:CDS | 25.0% |
| !! | AATCTTTACCCTTTTTGTAT+TGG | + | chr4.3:20016171-20016190 | None:intergenic | 25.0% |
| !! | ATCTTTACCCTTTTTGTATT+GGG | + | chr4.3:20016170-20016189 | None:intergenic | 25.0% |
| CAAAGTTACCCAATACAAAA+AGG | - | chr4.3:20016159-20016178 | MS.gene70395:CDS | 30.0% | |
| CTACTAAGAAAATTGTGTTG+AGG | - | chr4.3:20016384-20016403 | MS.gene70395:CDS | 30.0% | |
| TATCGTTGTGTGTTTGAATT+AGG | - | chr4.3:20016355-20016374 | MS.gene70395:CDS | 30.0% | |
| ! | TAGTACCTTTTTGTGAAAGA+CGG | + | chr4.3:20016260-20016279 | None:intergenic | 30.0% |
| !! | GATATTAGATGAAAGTGTTG+TGG | + | chr4.3:20016015-20016034 | None:intergenic | 30.0% |
| GTATTGGGTAACTTTGTGTA+AGG | + | chr4.3:20016155-20016174 | None:intergenic | 35.0% | |
| TGTTTCACAACACGCAATTA+AGG | - | chr4.3:20016430-20016449 | MS.gene70395:CDS | 35.0% | |
| ! | AGTACCTTTTTGTGAAAGAC+GGG | + | chr4.3:20016259-20016278 | None:intergenic | 35.0% |
| ! | CAGTAGGTTTTCTTTGTCTT+TGG | + | chr4.3:20016106-20016125 | None:intergenic | 35.0% |
| !! | GTTTTTGTTTGTGTTTGCAG+AGG | - | chr4.3:20016524-20016543 | MS.gene70395:intron | 35.0% |
| !! | TTAGATGAAAGTGTTGTGGA+AGG | + | chr4.3:20016011-20016030 | None:intergenic | 35.0% |
| !!! | TTACTGTTTTTGGGTTCTGT+GGG | - | chr4.3:20016492-20016511 | MS.gene70395:intron | 35.0% |
| !!! | TTTACTGTTTTTGGGTTCTG+TGG | - | chr4.3:20016491-20016510 | MS.gene70395:intron | 35.0% |
| AAGGTTAGATGAGGGAAATG+AGG | + | chr4.3:20015992-20016011 | None:intergenic | 40.0% | |
| CAAACCCGTCTTTCACAAAA+AGG | - | chr4.3:20016252-20016271 | MS.gene70395:intron | 40.0% | |
| CACCATAACCAGATTGTTTG+CGG | + | chr4.3:20016225-20016244 | None:intergenic | 40.0% | |
| GAAAACCTACTGCAAAAGCA+AGG | - | chr4.3:20016114-20016133 | MS.gene70395:intron | 40.0% | |
| TGTTGTGGAAGGTTAGATGA+GGG | + | chr4.3:20016000-20016019 | None:intergenic | 40.0% | |
| !! | CTTTTTGTGAAAGACGGGTT+TGG | + | chr4.3:20016254-20016273 | None:intergenic | 40.0% |
| !!! | TTTTGGGTTCTGTGGGTAAT+GGG | - | chr4.3:20016499-20016518 | MS.gene70395:intron | 40.0% |
| !!! | TTTTTGGGTTCTGTGGGTAA+TGG | - | chr4.3:20016498-20016517 | MS.gene70395:intron | 40.0% |
| AAAGATTCCATCGCTGCTCA+GGG | - | chr4.3:20016184-20016203 | MS.gene70395:CDS | 45.0% | |
| CGCAAACAATCTGGTTATGG+TGG | - | chr4.3:20016223-20016242 | MS.gene70395:intron | 45.0% | |
| CGTTATGACCGCAAACAATC+TGG | - | chr4.3:20016214-20016233 | MS.gene70395:intron | 45.0% | |
| GACCGCAAACAATCTGGTTA+TGG | - | chr4.3:20016220-20016239 | MS.gene70395:intron | 45.0% | |
| GTGTTGTGGAAGGTTAGATG+AGG | + | chr4.3:20016001-20016020 | None:intergenic | 45.0% | |
| TAAAGATTCCATCGCTGCTC+AGG | - | chr4.3:20016183-20016202 | MS.gene70395:CDS | 45.0% | |
| !! | AGGTGCAAGCACTTTGAGAT+TGG | - | chr4.3:20016544-20016563 | MS.gene70395:intron | 45.0% |
| !! | GAGATTGGTGGTGACAAGAA+GGG | - | chr4.3:20016559-20016578 | MS.gene70395:intron | 45.0% |
| !! | TGAGATTGGTGGTGACAAGA+AGG | - | chr4.3:20016558-20016577 | MS.gene70395:intron | 45.0% |
| !! | TGCAAGCACTTTGAGATTGG+TGG | - | chr4.3:20016547-20016566 | MS.gene70395:intron | 45.0% |
| GGTGGTGACAAGAAGGGAAA+AGG | - | chr4.3:20016565-20016584 | MS.gene70395:intron | 50.0% | |
| GTGTTGAGGCTTCAGTGTCA+AGG | - | chr4.3:20016398-20016417 | MS.gene70395:CDS | 50.0% | |
| !! | GCACTCCTTGCTTTTGCAGT+AGG | + | chr4.3:20016122-20016141 | None:intergenic | 50.0% |
| ! | ACGTTTTCCCTGAGCAGCGA+TGG | + | chr4.3:20016194-20016213 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 20015979 | 20016603 | 20015979 | ID=MS.gene70395 |
| chr4.3 | mRNA | 20015979 | 20016603 | 20015979 | ID=MS.gene70395.t1;Parent=MS.gene70395 |
| chr4.3 | exon | 20016601 | 20016603 | 20016601 | ID=MS.gene70395.t1.exon1;Parent=MS.gene70395.t1 |
| chr4.3 | CDS | 20016601 | 20016603 | 20016601 | ID=cds.MS.gene70395.t1;Parent=MS.gene70395.t1 |
| chr4.3 | exon | 20016309 | 20016488 | 20016309 | ID=MS.gene70395.t1.exon2;Parent=MS.gene70395.t1 |
| chr4.3 | CDS | 20016309 | 20016488 | 20016309 | ID=cds.MS.gene70395.t1;Parent=MS.gene70395.t1 |
| chr4.3 | exon | 20016131 | 20016205 | 20016131 | ID=MS.gene70395.t1.exon3;Parent=MS.gene70395.t1 |
| chr4.3 | CDS | 20016131 | 20016205 | 20016131 | ID=cds.MS.gene70395.t1;Parent=MS.gene70395.t1 |
| chr4.3 | exon | 20015979 | 20016038 | 20015979 | ID=MS.gene70395.t1.exon4;Parent=MS.gene70395.t1 |
| chr4.3 | CDS | 20015979 | 20016038 | 20015979 | ID=cds.MS.gene70395.t1;Parent=MS.gene70395.t1 |
| Gene Sequence |
| Protein sequence |
