AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051330.t1 MTR_7g100280 100.000 105 0 0 1 105 1 105 1.57e-71 208
MS.gene051330.t1 MTR_4g094308 100.000 105 0 0 1 105 1 105 1.57e-71 208
MS.gene051330.t1 MTR_2g008180 100.000 105 0 0 1 105 1 105 1.57e-71 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051330.t1 AT4G14320 92.381 105 8 0 1 105 1 105 1.00e-66 196
MS.gene051330.t1 AT3G23390 92.381 105 8 0 1 105 1 105 1.00e-66 196
MS.gene051330.t1 AT4G14320 91.398 93 8 0 1 93 1 93 3.30e-57 174

Find 18 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGTTACCCAATACAAAA+AGG 0.271604 4.1:-19333196 MS.gene051330:CDS
TGTTTCACAACACGCAATTA+AGG 0.365888 4.1:-19332921 MS.gene051330:intron
CAAACCCGTCTTTCACAAAA+AGG 0.369511 4.1:-19333103 MS.gene051330:intron
TAAAGATTCCATCGCTGCTC+AGG 0.399194 4.1:-19333172 MS.gene051330:CDS
CGTTATGACCGCAAACAATC+TGG 0.415551 4.1:-19333141 MS.gene051330:CDS
GTATTGGGTAACTTTGTGTA+AGG 0.453756 4.1:+19333203 None:intergenic
CACCATAACCAGATTGTTTG+CGG 0.454458 4.1:+19333133 None:intergenic
GACCGCAAACAATCTGGTTA+TGG 0.454865 4.1:-19333135 MS.gene051330:CDS
AGGTGCAAGCACTTTGAGAT+TGG 0.462716 4.1:-19332817 MS.gene051330:CDS
TGCAAGCACTTTGAGATTGG+TGG 0.497179 4.1:-19332814 MS.gene051330:CDS
CGCAAACAATCTGGTTATGG+TGG 0.541862 4.1:-19333132 MS.gene051330:CDS
TGAGATTGGTGGCGACAAGA+AGG 0.552379 4.1:-19332803 MS.gene051330:CDS
GAGATTGGTGGCGACAAGAA+GGG 0.552573 4.1:-19332802 MS.gene051330:CDS
GGTGGCGACAAGAAGGGAAA+AGG 0.559082 4.1:-19332796 MS.gene051330:CDS
CTACTAAGAAAATTGTGTTG+AGG 0.599248 4.1:-19332967 MS.gene051330:CDS
AAAGATTCCATCGCTGCTCA+GGG 0.607025 4.1:-19333171 MS.gene051330:CDS
GAAAACCTACTGCAAAAGCA+AGG 0.613372 4.1:-19333241 MS.gene051330:CDS
GTGTTGAGGCTTCAGTGTCA+AGG 0.618711 4.1:-19332953 MS.gene051330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTCAATTTTTGTTGTTAT+AGG - chr4.1:19332873-19332892 MS.gene051330:intron 15.0%
!!! ATAAATGAAATTGTTGATAA+TGG + chr4.1:19332831-19332850 None:intergenic 15.0%
!! ATCAAATTAAGAAAAACAGA+AGG + chr4.1:19333106-19333125 None:intergenic 20.0%
! AAAGTTACCCAATACAAAAA+GGG - chr4.1:19332961-19332980 MS.gene051330:CDS 25.0%
! TATTGTTGTGTGTTTGAATT+AGG - chr4.1:19333160-19333179 MS.gene051330:CDS 25.0%
!! AATCTTTACCCTTTTTGTAT+TGG + chr4.1:19332972-19332991 None:intergenic 25.0%
!! ATCTTTACCCTTTTTGTATT+GGG + chr4.1:19332971-19332990 None:intergenic 25.0%
!!! GTGTAATTTGTTCTGATTTT+GGG - chr4.1:19333278-19333297 MS.gene051330:intron 25.0%
!!! TGTGTAATTTGTTCTGATTT+TGG - chr4.1:19333277-19333296 MS.gene051330:intron 25.0%
AAACAGAAGGTAAAGACTTA+AGG + chr4.1:19333093-19333112 None:intergenic 30.0%
CAAAGTTACCCAATACAAAA+AGG - chr4.1:19332960-19332979 MS.gene051330:CDS 30.0%
CTACTAAGAAAATTGTGTTG+AGG - chr4.1:19333189-19333208 MS.gene051330:CDS 30.0%
! TTGTACCTTTTTGTGAAAGA+CGG + chr4.1:19333061-19333080 None:intergenic 30.0%
GTATTGGGTAACTTTGTGTA+AGG + chr4.1:19332956-19332975 None:intergenic 35.0%
TGTTTCACAACACGCAATTA+AGG - chr4.1:19333235-19333254 MS.gene051330:CDS 35.0%
! CAGTAGGTTTTCTTTGTCTT+TGG + chr4.1:19332907-19332926 None:intergenic 35.0%
! TGTACCTTTTTGTGAAAGAC+GGG + chr4.1:19333060-19333079 None:intergenic 35.0%
!! GATAATGGATGAAAGTGTTG+TGG + chr4.1:19332816-19332835 None:intergenic 35.0%
!! GTTTTTGTTTGTGTTTGCAG+AGG - chr4.1:19333319-19333338 MS.gene051330:intron 35.0%
CAAACCCGTCTTTCACAAAA+AGG - chr4.1:19333053-19333072 MS.gene051330:intron 40.0%
CACCATAACCAGATTGTTTG+CGG + chr4.1:19333026-19333045 None:intergenic 40.0%
GAAAACCTACTGCAAAAGCA+AGG - chr4.1:19332915-19332934 MS.gene051330:intron 40.0%
TGTTGTGGAAGGTTAGATGA+GGG + chr4.1:19332801-19332820 None:intergenic 40.0%
!! ATGGATGAAAGTGTTGTGGA+AGG + chr4.1:19332812-19332831 None:intergenic 40.0%
!! CTTTTTGTGAAAGACGGGTT+TGG + chr4.1:19333055-19333074 None:intergenic 40.0%
!!! ATTTTGGGTTCTGTGGGTAA+TGG - chr4.1:19333293-19333312 MS.gene051330:intron 40.0%
!!! GTTCTGATTTTGGGTTCTGT+GGG - chr4.1:19333287-19333306 MS.gene051330:intron 40.0%
!!! TGTTCTGATTTTGGGTTCTG+TGG - chr4.1:19333286-19333305 MS.gene051330:intron 40.0%
!!! TTTTGGGTTCTGTGGGTAAT+GGG - chr4.1:19333294-19333313 MS.gene051330:intron 40.0%
AAAGATTCCATCGCTGCTCA+GGG - chr4.1:19332985-19333004 MS.gene051330:intron 45.0%
CGCAAACAATCTGGTTATGG+TGG - chr4.1:19333024-19333043 MS.gene051330:intron 45.0%
CGTTATGACCGCAAACAATC+TGG - chr4.1:19333015-19333034 MS.gene051330:intron 45.0%
GACCGCAAACAATCTGGTTA+TGG - chr4.1:19333021-19333040 MS.gene051330:intron 45.0%
GTGTTGTGGAAGGTTAGATG+AGG + chr4.1:19332802-19332821 None:intergenic 45.0%
TAAAGATTCCATCGCTGCTC+AGG - chr4.1:19332984-19333003 MS.gene051330:intron 45.0%
!! AGGTGCAAGCACTTTGAGAT+TGG - chr4.1:19333339-19333358 MS.gene051330:intron 45.0%
!! TGCAAGCACTTTGAGATTGG+TGG - chr4.1:19333342-19333361 MS.gene051330:intron 45.0%
GTGTTGAGGCTTCAGTGTCA+AGG - chr4.1:19333203-19333222 MS.gene051330:CDS 50.0%
!! GAGATTGGTGGCGACAAGAA+GGG - chr4.1:19333354-19333373 MS.gene051330:intron 50.0%
!! GCACTCCTTGCTTTTGCAGT+AGG + chr4.1:19332923-19332942 None:intergenic 50.0%
!! TGAGATTGGTGGCGACAAGA+AGG - chr4.1:19333353-19333372 MS.gene051330:intron 50.0%
GGTGGCGACAAGAAGGGAAA+AGG - chr4.1:19333360-19333379 MS.gene051330:intron 55.0%
! ACGTTTTCCCTGAGCAGCGA+TGG + chr4.1:19332995-19333014 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 19332780 19333398 19332780 ID=MS.gene051330
chr4.1 mRNA 19332780 19333398 19332780 ID=MS.gene051330.t1;Parent=MS.gene051330
chr4.1 exon 19333396 19333398 19333396 ID=MS.gene051330.t1.exon1;Parent=MS.gene051330.t1
chr4.1 CDS 19333396 19333398 19333396 ID=cds.MS.gene051330.t1;Parent=MS.gene051330.t1
chr4.1 exon 19333104 19333283 19333104 ID=MS.gene051330.t1.exon2;Parent=MS.gene051330.t1
chr4.1 CDS 19333104 19333283 19333104 ID=cds.MS.gene051330.t1;Parent=MS.gene051330.t1
chr4.1 exon 19332922 19332996 19332922 ID=MS.gene051330.t1.exon3;Parent=MS.gene051330.t1
chr4.1 CDS 19332922 19332996 19332922 ID=cds.MS.gene051330.t1;Parent=MS.gene051330.t1
chr4.1 exon 19332780 19332839 19332780 ID=MS.gene051330.t1.exon4;Parent=MS.gene051330.t1
chr4.1 CDS 19332780 19332839 19332780 ID=cds.MS.gene051330.t1;Parent=MS.gene051330.t1
Gene Sequence

>MS.gene051330

ATGGTAAACTACTCATTTCCCTCATCTAACCTTCCACAACACTTTCATCCATTATCAACAATTTCATTTATAGATCTCACTTTATGTCAAATTAATTCAATTTTTGTTGTTATAGGTGAACGTTCCAAAGACAAAGAAAACCTACTGCAAAAGCAAGGAGTGCAAGAAGCACACCTTACACAAAGTTACCCAATACAAAAAGGGTAAAGATTCCATCGCTGCTCAGGGAAAACGTCGTTATGACCGCAAACAATCTGGTTATGGTGGACAGACCAAACCCGTCTTTCACAAAAAGGTACAATTGCTGTTACCTTAAGTCTTTACCTTCTGTTTTTCTTAATTTGATTAGTAATTAAGATGTTAATTAAGTTATTTGTTTTTATTGTTGTGTGTTTGAATTAGGCAAAAACTACTAAGAAAATTGTGTTGAGGCTTCAGTGTCAAGGTTGCAAGCATGTTTCACAACACGCAATTAAGGTAAGCGTGTTTGTTTTTGTTGTGTAATTTGTTCTGATTTTGGGTTCTGTGGGTAATGGGATGTTTTTGTTTGTGTTTGCAGAGGTGCAAGCACTTTGAGATTGGTGGCGACAAGAAGGGAAAAGGAACATCTCTGTTTTAG

Protein sequence

>MS.gene051330.t1

MVNVPKTKKTYCKSKECKKHTLHKVTQYKKGKDSIAAQGKRRYDRKQSGYGGQTKPVFHKKAKTTKKIVLRLQCQGCKHVSQHAIKRCKHFEIGGDKKGKGTSLF