Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071958.t1 | XP_003593138.1 | 100 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 6.00E-53 | 216.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071958.t1 | Q96499 | 97.1 | 105 | 3 | 0 | 1 | 105 | 1 | 105 | 2.1e-54 | 212.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071958.t1 | A0A2K3MQ78 | 100.0 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 4.3e-53 | 216.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049129 | MS.gene071958 | -0.811611 | 6.42E-51 | -1.69E-46 |
| MS.gene049255 | MS.gene071958 | 0.860686 | 1.74E-63 | -1.69E-46 |
| MS.gene049411 | MS.gene071958 | 0.802896 | 4.51E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene071958 | 0.835668 | 1.47E-56 | -1.69E-46 |
| MS.gene050290 | MS.gene071958 | 0.806507 | 7.95E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene071958 | 0.848269 | 6.82E-60 | -1.69E-46 |
| MS.gene050838 | MS.gene071958 | 0.854467 | 1.21E-61 | -1.69E-46 |
| MS.gene050916 | MS.gene071958 | 0.856492 | 3.10E-62 | -1.69E-46 |
| MS.gene050955 | MS.gene071958 | 0.841539 | 4.47E-58 | -1.69E-46 |
| MS.gene051003 | MS.gene071958 | 0.82673 | 2.31E-54 | -1.69E-46 |
| MS.gene051234 | MS.gene071958 | 0.852865 | 3.49E-61 | -1.69E-46 |
| MS.gene051328 | MS.gene071958 | 0.820663 | 6.09E-53 | -1.69E-46 |
| MS.gene051330 | MS.gene071958 | 0.824355 | 8.44E-54 | -1.69E-46 |
| MS.gene05134 | MS.gene071958 | 0.832543 | 8.91E-56 | -1.69E-46 |
| MS.gene051746 | MS.gene071958 | 0.833841 | 4.23E-56 | -1.69E-46 |
| MS.gene051748 | MS.gene071958 | 0.812716 | 3.69E-51 | -1.69E-46 |
| MS.gene051791 | MS.gene071958 | 0.810488 | 1.12E-50 | -1.69E-46 |
| MS.gene051850 | MS.gene071958 | 0.844319 | 8.14E-59 | -1.69E-46 |
| MS.gene05188 | MS.gene071958 | 0.83168 | 1.46E-55 | -1.69E-46 |
| MS.gene051903 | MS.gene071958 | 0.847082 | 1.45E-59 | -1.69E-46 |
| MS.gene052133 | MS.gene071958 | 0.832058 | 1.17E-55 | -1.69E-46 |
| MS.gene052947 | MS.gene071958 | 0.88569 | 6.54E-72 | -1.69E-46 |
| MS.gene053122 | MS.gene071958 | 0.82681 | 2.21E-54 | -1.69E-46 |
| MS.gene053952 | MS.gene071958 | 0.803383 | 3.58E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene071958 | 0.850292 | 1.87E-60 | -1.69E-46 |
| MS.gene054937 | MS.gene071958 | 0.862197 | 6.01E-64 | -1.69E-46 |
| MS.gene055162 | MS.gene071958 | 0.807915 | 4.00E-50 | -1.69E-46 |
| MS.gene055555 | MS.gene071958 | 0.814225 | 1.72E-51 | -1.69E-46 |
| MS.gene055808 | MS.gene071958 | 0.811702 | 6.13E-51 | -1.69E-46 |
| MS.gene056075 | MS.gene071958 | 0.894805 | 1.74E-75 | -1.69E-46 |
| MS.gene056215 | MS.gene071958 | 0.819407 | 1.18E-52 | -1.69E-46 |
| MS.gene056631 | MS.gene071958 | 0.842417 | 2.62E-58 | -1.69E-46 |
| MS.gene056917 | MS.gene071958 | 0.80845 | 3.08E-50 | -1.69E-46 |
| MS.gene057094 | MS.gene071958 | 0.834745 | 2.51E-56 | -1.69E-46 |
| MS.gene057206 | MS.gene071958 | 0.82917 | 5.98E-55 | -1.69E-46 |
| MS.gene057705 | MS.gene071958 | 0.878419 | 2.85E-69 | -1.69E-46 |
| MS.gene058364 | MS.gene071958 | 0.825451 | 4.65E-54 | -1.69E-46 |
| MS.gene059146 | MS.gene071958 | 0.80936 | 1.97E-50 | -1.69E-46 |
| MS.gene059227 | MS.gene071958 | 0.812486 | 4.14E-51 | -1.69E-46 |
| MS.gene06001 | MS.gene071958 | 0.873392 | 1.52E-67 | -1.69E-46 |
| MS.gene06011 | MS.gene071958 | 0.804534 | 2.06E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene071958 | 0.871625 | 5.93E-67 | -1.69E-46 |
| MS.gene060763 | MS.gene071958 | 0.814014 | 1.91E-51 | -1.69E-46 |
| MS.gene060917 | MS.gene071958 | 0.828489 | 8.74E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene047093 | MS.gene071958 | PPI |
| MS.gene071958 | MS.gene35046 | PPI |
| MS.gene051330 | MS.gene071958 | PPI |
| MS.gene70395 | MS.gene071958 | PPI |
| MS.gene071958 | MS.gene01133 | PPI |
| MS.gene002165 | MS.gene071958 | PPI |
| MS.gene071958 | MS.gene028410 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071958.t1 | MTR_7g100280 | 100.000 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.57e-71 | 208 |
| MS.gene071958.t1 | MTR_4g094308 | 100.000 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.57e-71 | 208 |
| MS.gene071958.t1 | MTR_2g008180 | 100.000 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.57e-71 | 208 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071958.t1 | AT4G14320 | 92.381 | 105 | 8 | 0 | 1 | 105 | 1 | 105 | 1.00e-66 | 196 |
| MS.gene071958.t1 | AT3G23390 | 92.381 | 105 | 8 | 0 | 1 | 105 | 1 | 105 | 1.00e-66 | 196 |
| MS.gene071958.t1 | AT4G14320 | 91.398 | 93 | 8 | 0 | 1 | 93 | 1 | 93 | 3.30e-57 | 174 |
Find 25 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTTACCCTTCTTGTATT+GGG | 0.247979 | 2.4:-74763851 | None:intergenic |
| TATCTTTACCCTTCTTGTAT+TGG | 0.312780 | 2.4:-74763852 | None:intergenic |
| CAAGGGAACTTCTCTCTTCT+AGG | 0.380957 | 2.4:+74764718 | MS.gene071958:CDS |
| GATCGCAAACAGTCTGGTTA+TGG | 0.387570 | 2.4:+74763904 | MS.gene071958:CDS |
| TGTTCTGAGGTTGCAATGCC+AGG | 0.414173 | 2.4:+74764508 | MS.gene071958:CDS |
| GCATTCCTTGCTCTTGCAGT+AGG | 0.441918 | 2.4:-74763803 | None:intergenic |
| TGAGATTGGTGGTGATAAGA+AGG | 0.451732 | 2.4:+74764694 | MS.gene071958:CDS |
| CGTTACGATCGCAAACAGTC+TGG | 0.464861 | 2.4:+74763898 | MS.gene071958:CDS |
| TGTTTCTCAACATGCAATCA+AGG | 0.468902 | 2.4:+74764541 | MS.gene071958:CDS |
| GTTACCTTCTTGTGAAAGAC+GGG | 0.495280 | 2.4:-74763940 | None:intergenic |
| GAGAAACATGCTTGCAACCC+TGG | 0.514679 | 2.4:-74764526 | None:intergenic |
| GAGATTGGTGGTGATAAGAA+GGG | 0.526133 | 2.4:+74764695 | MS.gene071958:CDS |
| CAAGGTTACCCAATACAAGA+AGG | 0.553515 | 2.4:+74763843 | MS.gene071958:CDS |
| CGCAAACAGTCTGGTTATGG+TGG | 0.554816 | 2.4:+74763907 | MS.gene071958:CDS |
| TGGTGGTGATAAGAAGGGCA+AGG | 0.556388 | 2.4:+74764700 | MS.gene071958:CDS |
| AAAGATAGCATCGCCGCTCA+AGG | 0.571046 | 2.4:+74763868 | MS.gene071958:CDS |
| AGTTACCTTCTTGTGAAAGA+CGG | 0.572609 | 2.4:-74763941 | None:intergenic |
| CAAGAAGCATACACTTCACA+AGG | 0.596444 | 2.4:+74763825 | MS.gene071958:CDS |
| GAAAACCTACTGCAAGAGCA+AGG | 0.601503 | 2.4:+74763798 | MS.gene071958:CDS |
| CCACTAAGAAAATTGTTCTG+AGG | 0.602363 | 2.4:+74764495 | MS.gene071958:CDS |
| TAAGCCCGTCTTTCACAAGA+AGG | 0.606865 | 2.4:+74763936 | MS.gene071958:CDS |
| AAGGTTACCCAATACAAGAA+GGG | 0.630634 | 2.4:+74763844 | MS.gene071958:CDS |
| GGTGGTGATAAGAAGGGCAA+GGG | 0.630826 | 2.4:+74764701 | MS.gene071958:CDS |
| GTAACGACGCTTACCTTGAG+CGG | 0.638526 | 2.4:-74763881 | None:intergenic |
| GTTCTGAGGTTGCAATGCCA+GGG | 0.641034 | 2.4:+74764509 | MS.gene071958:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTAATTTTTGTTTGTGTT+TGG | + | chr2.4:74764452-74764471 | MS.gene071958:intron | 15.0% |
| !!! | GATTTTTTTTTTTAGTTGAA+TGG | + | chr2.4:74764422-74764441 | MS.gene071958:intron | 15.0% |
| !! | AAATGTATAAAAGTAATGAG+GGG | - | chr2.4:74763978-74763997 | None:intergenic | 20.0% |
| !! | CTTACAATCAACTAAAAAAA+TGG | - | chr2.4:74764022-74764041 | None:intergenic | 20.0% |
| !! | GAAATGTATAAAAGTAATGA+GGG | - | chr2.4:74763979-74763998 | None:intergenic | 20.0% |
| !! | GAATGCAAAAAATTCAAATT+TGG | + | chr2.4:74764345-74764364 | MS.gene071958:intron | 20.0% |
| !! | TGAAATGTATAAAAGTAATG+AGG | - | chr2.4:74763980-74763999 | None:intergenic | 20.0% |
| !!! | CAGAAATTTTATTTTGTAGA+TGG | + | chr2.4:74764603-74764622 | MS.gene071958:intron | 20.0% |
| !!! | TTTTATTTTGTAGATGGATA+TGG | + | chr2.4:74764609-74764628 | MS.gene071958:intron | 20.0% |
| ! | AATGTATAAAAGTAATGAGG+GGG | - | chr2.4:74763977-74763996 | None:intergenic | 25.0% |
| ! | ATATGGTTGTTTGTATCTAA+TGG | + | chr2.4:74764626-74764645 | MS.gene071958:intron | 25.0% |
| ! | GAAAATCCAACATCAAAAAA+GGG | - | chr2.4:74763756-74763775 | None:intergenic | 25.0% |
| ! | TGAAAATCCAACATCAAAAA+AGG | - | chr2.4:74763757-74763776 | None:intergenic | 25.0% |
| ! | TGATATAATTACGATTACGT+AGG | - | chr2.4:74763725-74763744 | None:intergenic | 25.0% |
| ! | TGGATTCAAATATGGTTAAT+AGG | + | chr2.4:74764146-74764165 | MS.gene071958:intron | 25.0% |
| !!! | TAGCTTTATGCAAATGATTA+GGG | + | chr2.4:74764103-74764122 | MS.gene071958:intron | 25.0% |
| !!! | TGTGTTTGGTTTTATTGTAT+AGG | + | chr2.4:74764466-74764485 | MS.gene071958:intron | 25.0% |
| !!! | TTAGCTTTATGCAAATGATT+AGG | + | chr2.4:74764102-74764121 | MS.gene071958:intron | 25.0% |
| !!! | TTTTTTGATGTTGGATTTTC+AGG | + | chr2.4:74763756-74763775 | MS.gene071958:intron | 25.0% |
| ATCTTTACCCTTCTTGTATT+GGG | - | chr2.4:74763854-74763873 | None:intergenic | 30.0% | |
| GGTTAATAGGAAGAACATTA+TGG | + | chr2.4:74764159-74764178 | MS.gene071958:intron | 30.0% | |
| TATCTTTACCCTTCTTGTAT+TGG | - | chr2.4:74763855-74763874 | None:intergenic | 30.0% | |
| ! | TTTTGAGCTGAATGTGAAAT+TGG | + | chr2.4:74764258-74764277 | MS.gene071958:intron | 30.0% |
| AAGGTTACCCAATACAAGAA+GGG | + | chr2.4:74763844-74763863 | MS.gene071958:CDS | 35.0% | |
| AGGGAAGATTTAGAGATTGA+TGG | + | chr2.4:74764122-74764141 | MS.gene071958:intron | 35.0% | |
| AGTTACCTTCTTGTGAAAGA+CGG | - | chr2.4:74763944-74763963 | None:intergenic | 35.0% | |
| CAAACCCACTTAGTGATATA+AGG | + | chr2.4:74764202-74764221 | MS.gene071958:intron | 35.0% | |
| CCACTAAGAAAATTGTTCTG+AGG | + | chr2.4:74764495-74764514 | MS.gene071958:CDS | 35.0% | |
| TGTTTCTCAACATGCAATCA+AGG | + | chr2.4:74764541-74764560 | MS.gene071958:CDS | 35.0% | |
| ! | AAGATTTAGAGATTGATGGC+TGG | + | chr2.4:74764126-74764145 | MS.gene071958:intron | 35.0% |
| ! | CAAGCCTTATATCACTAAGT+GGG | - | chr2.4:74764209-74764228 | None:intergenic | 35.0% |
| ! | CAGTAGGTTTTCTTTGTCTT+TGG | - | chr2.4:74763790-74763809 | None:intergenic | 35.0% |
| ! | CCTCAGAACAATTTTCTTAG+TGG | - | chr2.4:74764498-74764517 | None:intergenic | 35.0% |
| ! | CTTATATCACTAAGTGGGTT+TGG | - | chr2.4:74764204-74764223 | None:intergenic | 35.0% |
| ! | TTGATGGCTGGATTCAAATA+TGG | + | chr2.4:74764138-74764157 | MS.gene071958:intron | 35.0% |
| !!! | GCTAAACCCTTTTTTGATGT+TGG | + | chr2.4:74763747-74763766 | MS.gene071958:intron | 35.0% |
| CAAGGTTACCCAATACAAGA+AGG | + | chr2.4:74763843-74763862 | MS.gene071958:CDS | 40.0% | |
| CCACTTAGTGATATAAGGCT+TGG | + | chr2.4:74764207-74764226 | MS.gene071958:intron | 40.0% | |
| GTTACCTTCTTGTGAAAGAC+GGG | - | chr2.4:74763943-74763962 | None:intergenic | 40.0% | |
| ! | CAAGAAGCATACACTTCACA+AGG | + | chr2.4:74763825-74763844 | MS.gene071958:CDS | 40.0% |
| ! | CCAAGCCTTATATCACTAAG+TGG | - | chr2.4:74764210-74764229 | None:intergenic | 40.0% |
| ! | GTTGTGTTTCTGTTATGCAG+AGG | + | chr2.4:74764660-74764679 | MS.gene071958:intron | 40.0% |
| !! | GAGATTGGTGGTGATAAGAA+GGG | + | chr2.4:74764695-74764714 | MS.gene071958:CDS | 40.0% |
| !! | TGAGATTGGTGGTGATAAGA+AGG | + | chr2.4:74764694-74764713 | MS.gene071958:CDS | 40.0% |
| !!! | AGGTGCAAGCATTTTGAGAT+TGG | + | chr2.4:74764680-74764699 | MS.gene071958:CDS | 40.0% |
| !!! | TGCAAGCATTTTGAGATTGG+TGG | + | chr2.4:74764683-74764702 | MS.gene071958:CDS | 40.0% |
| AAAGTAATGAGGGGGTGAAG+TGG | - | chr2.4:74763969-74763988 | None:intergenic | 45.0% | |
| AAGTAATGAGGGGGTGAAGT+GGG | - | chr2.4:74763968-74763987 | None:intergenic | 45.0% | |
| CGATTACGTAGGAACAGACA+CGG | - | chr2.4:74763714-74763733 | None:intergenic | 45.0% | |
| GAAAACCTACTGCAAGAGCA+AGG | + | chr2.4:74763798-74763817 | MS.gene071958:CDS | 45.0% | |
| GATCGCAAACAGTCTGGTTA+TGG | + | chr2.4:74763904-74763923 | MS.gene071958:CDS | 45.0% | |
| TAAGCCCGTCTTTCACAAGA+AGG | + | chr2.4:74763936-74763955 | MS.gene071958:CDS | 45.0% | |
| AAAGATAGCATCGCCGCTCA+AGG | + | chr2.4:74763868-74763887 | MS.gene071958:CDS | 50.0% | |
| CGCAAACAGTCTGGTTATGG+TGG | + | chr2.4:74763907-74763926 | MS.gene071958:CDS | 50.0% | |
| CGTTACGATCGCAAACAGTC+TGG | + | chr2.4:74763898-74763917 | MS.gene071958:CDS | 50.0% | |
| GAGAAACATGCTTGCAACCC+TGG | - | chr2.4:74764529-74764548 | None:intergenic | 50.0% | |
| GCATTCCTTGCTCTTGCAGT+AGG | - | chr2.4:74763806-74763825 | None:intergenic | 50.0% | |
| GGTGGTGATAAGAAGGGCAA+GGG | + | chr2.4:74764701-74764720 | MS.gene071958:CDS | 50.0% | |
| GTAACGACGCTTACCTTGAG+CGG | - | chr2.4:74763884-74763903 | None:intergenic | 50.0% | |
| TTACGTAGGAACAGACACGG+TGG | - | chr2.4:74763711-74763730 | None:intergenic | 50.0% | |
| ! | TGGTGGTGATAAGAAGGGCA+AGG | + | chr2.4:74764700-74764719 | MS.gene071958:CDS | 50.0% |
| !! | GTTCTGAGGTTGCAATGCCA+GGG | + | chr2.4:74764509-74764528 | MS.gene071958:CDS | 50.0% |
| !! | TGTTCTGAGGTTGCAATGCC+AGG | + | chr2.4:74764508-74764527 | MS.gene071958:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 74763695 | 74764739 | 74763695 | ID=MS.gene071958 |
| chr2.4 | mRNA | 74763695 | 74764739 | 74763695 | ID=MS.gene071958.t1;Parent=MS.gene071958 |
| chr2.4 | exon | 74763695 | 74763697 | 74763695 | ID=MS.gene071958.t1.exon1;Parent=MS.gene071958.t1 |
| chr2.4 | CDS | 74763695 | 74763697 | 74763695 | ID=cds.MS.gene071958.t1;Parent=MS.gene071958.t1 |
| chr2.4 | exon | 74763778 | 74763957 | 74763778 | ID=MS.gene071958.t1.exon2;Parent=MS.gene071958.t1 |
| chr2.4 | CDS | 74763778 | 74763957 | 74763778 | ID=cds.MS.gene071958.t1;Parent=MS.gene071958.t1 |
| chr2.4 | exon | 74764488 | 74764562 | 74764488 | ID=MS.gene071958.t1.exon3;Parent=MS.gene071958.t1 |
| chr2.4 | CDS | 74764488 | 74764562 | 74764488 | ID=cds.MS.gene071958.t1;Parent=MS.gene071958.t1 |
| chr2.4 | exon | 74764680 | 74764739 | 74764680 | ID=MS.gene071958.t1.exon4;Parent=MS.gene071958.t1 |
| chr2.4 | CDS | 74764680 | 74764739 | 74764680 | ID=cds.MS.gene071958.t1;Parent=MS.gene071958.t1 |
| Gene Sequence |
| Protein sequence |
