Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35046.t1 | XP_003593138.1 | 100 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 6.00E-53 | 216.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35046.t1 | Q96499 | 97.1 | 105 | 3 | 0 | 1 | 105 | 1 | 105 | 2.1e-54 | 212.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35046.t1 | A0A2K3MQ78 | 100.0 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 4.3e-53 | 216.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene35046 | 0.838094 | 3.53E-57 | -1.69E-46 |
| MS.gene050916 | MS.gene35046 | 0.84184 | 3.72E-58 | -1.69E-46 |
| MS.gene051330 | MS.gene35046 | 0.893117 | 8.47E-75 | -1.69E-46 |
| MS.gene061145 | MS.gene35046 | 0.838291 | 3.14E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70395 | MS.gene35046 | PPI |
| MS.gene071958 | MS.gene35046 | PPI |
| MS.gene002165 | MS.gene35046 | PPI |
| MS.gene047093 | MS.gene35046 | PPI |
| MS.gene35046 | MS.gene01133 | PPI |
| MS.gene051330 | MS.gene35046 | PPI |
| MS.gene028410 | MS.gene35046 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35046.t1 | MTR_7g100280 | 100.000 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.57e-71 | 208 |
| MS.gene35046.t1 | MTR_4g094308 | 100.000 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.57e-71 | 208 |
| MS.gene35046.t1 | MTR_2g008180 | 100.000 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 1.57e-71 | 208 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35046.t1 | AT4G14320 | 92.381 | 105 | 8 | 0 | 1 | 105 | 1 | 105 | 1.00e-66 | 196 |
| MS.gene35046.t1 | AT3G23390 | 92.381 | 105 | 8 | 0 | 1 | 105 | 1 | 105 | 1.00e-66 | 196 |
| MS.gene35046.t1 | AT4G14320 | 91.398 | 93 | 8 | 0 | 1 | 93 | 1 | 93 | 3.30e-57 | 174 |
Find 18 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGTTACCCAATACAAAA+AGG | 0.271604 | 4.4:-20569531 | MS.gene35046:CDS |
| TGTTTCACAACACGCAATTA+AGG | 0.365888 | 4.4:-20569260 | MS.gene35046:intron |
| CAAACCCGTCTTTCACAAAA+AGG | 0.369511 | 4.4:-20569438 | MS.gene35046:intron |
| TAAAGATTCCATCGCTGCTC+AGG | 0.399194 | 4.4:-20569507 | MS.gene35046:CDS |
| CGTTATGACCGCAAACAATC+TGG | 0.415551 | 4.4:-20569476 | MS.gene35046:CDS |
| GTATTGGGTAACTTTGTGTA+AGG | 0.453756 | 4.4:+20569538 | None:intergenic |
| CACCATAACCAGATTGTTTG+CGG | 0.454458 | 4.4:+20569468 | None:intergenic |
| GACCGCAAACAATCTGGTTA+TGG | 0.454865 | 4.4:-20569470 | MS.gene35046:CDS |
| AGGTGCAAGCACTTTGAGAT+TGG | 0.462716 | 4.4:-20569146 | MS.gene35046:CDS |
| TGAGATTGGTGGTGACAAGA+AGG | 0.508837 | 4.4:-20569132 | MS.gene35046:CDS |
| CGCAAACAATCTGGTTATGG+TGG | 0.541862 | 4.4:-20569467 | MS.gene35046:CDS |
| GGTGGTGACAAGAAGGGAAA+AGG | 0.543946 | 4.4:-20569125 | MS.gene35046:CDS |
| TGCAAGCACTTTGAGATTGG+TGG | 0.545430 | 4.4:-20569143 | MS.gene35046:CDS |
| GAGATTGGTGGTGACAAGAA+GGG | 0.555011 | 4.4:-20569131 | MS.gene35046:CDS |
| CTACTAAGAAAATTGTGTTG+AGG | 0.599248 | 4.4:-20569306 | MS.gene35046:CDS |
| AAAGATTCCATCGCTGCTCA+GGG | 0.607025 | 4.4:-20569506 | MS.gene35046:CDS |
| GAAAACCTACTGCAAAAGCA+AGG | 0.613372 | 4.4:-20569576 | MS.gene35046:CDS |
| GTGTTGAGGCTTCAGTGTCA+AGG | 0.618711 | 4.4:-20569292 | MS.gene35046:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTCAATTTTTGTTGTTAT+AGG | - | chr4.4:20569202-20569221 | MS.gene35046:intron | 15.0% |
| !!! | ATAAATGAAATTGTTGATAA+TGG | + | chr4.4:20569160-20569179 | None:intergenic | 15.0% |
| !! | ATTAGTAATTAGTAATTTGG+AGG | - | chr4.4:20569442-20569461 | MS.gene35046:CDS | 20.0% |
| !! | CTGATTAGTAATTAGTAATT+TGG | - | chr4.4:20569439-20569458 | MS.gene35046:CDS | 20.0% |
| !!! | AGTGAAATTTTTACTGTTTT+TGG | - | chr4.4:20569612-20569631 | MS.gene35046:intron | 20.0% |
| !!! | GTGAAATTTTTACTGTTTTT+GGG | - | chr4.4:20569613-20569632 | MS.gene35046:intron | 20.0% |
| ! | AAAGTTACCCAATACAAAAA+GGG | - | chr4.4:20569290-20569309 | MS.gene35046:CDS | 25.0% |
| ! | TCAGATTAAGCAAAGATTTA+AGG | + | chr4.4:20569423-20569442 | None:intergenic | 25.0% |
| !! | AATCTTTACCCTTTTTGTAT+TGG | + | chr4.4:20569301-20569320 | None:intergenic | 25.0% |
| !! | ATCTTTACCCTTTTTGTATT+GGG | + | chr4.4:20569300-20569319 | None:intergenic | 25.0% |
| CAAAGTTACCCAATACAAAA+AGG | - | chr4.4:20569289-20569308 | MS.gene35046:CDS | 30.0% | |
| CTACTAAGAAAATTGTGTTG+AGG | - | chr4.4:20569514-20569533 | MS.gene35046:CDS | 30.0% | |
| TATCGTTGTGTGTTTGAATT+AGG | - | chr4.4:20569485-20569504 | MS.gene35046:CDS | 30.0% | |
| ! | TAGTACCTTTTTGTGAAAGA+CGG | + | chr4.4:20569390-20569409 | None:intergenic | 30.0% |
| GTATTGGGTAACTTTGTGTA+AGG | + | chr4.4:20569285-20569304 | None:intergenic | 35.0% | |
| TGTTTCACAACACGCAATTA+AGG | - | chr4.4:20569560-20569579 | MS.gene35046:CDS | 35.0% | |
| ! | AGTACCTTTTTGTGAAAGAC+GGG | + | chr4.4:20569389-20569408 | None:intergenic | 35.0% |
| ! | CAGTAGGTTTTCTTTGTCTT+TGG | + | chr4.4:20569236-20569255 | None:intergenic | 35.0% |
| !! | GATAATGGATGAAAGTGTTG+TGG | + | chr4.4:20569145-20569164 | None:intergenic | 35.0% |
| !! | GTTTTTGTTTGTGTTTGCAG+AGG | - | chr4.4:20569654-20569673 | MS.gene35046:intron | 35.0% |
| !!! | TTACTGTTTTTGGGTTCTGT+GGG | - | chr4.4:20569622-20569641 | MS.gene35046:intron | 35.0% |
| !!! | TTTACTGTTTTTGGGTTCTG+TGG | - | chr4.4:20569621-20569640 | MS.gene35046:intron | 35.0% |
| AAGGTAAGATGAGGGAAATG+AGG | + | chr4.4:20569122-20569141 | None:intergenic | 40.0% | |
| CAAACCCGTCTTTCACAAAA+AGG | - | chr4.4:20569382-20569401 | MS.gene35046:intron | 40.0% | |
| CACCATAACCAGATTGTTTG+CGG | + | chr4.4:20569355-20569374 | None:intergenic | 40.0% | |
| GAAAACCTACTGCAAAAGCA+AGG | - | chr4.4:20569244-20569263 | MS.gene35046:intron | 40.0% | |
| TGTTGTGGAAGGTAAGATGA+GGG | + | chr4.4:20569130-20569149 | None:intergenic | 40.0% | |
| !! | ATGGATGAAAGTGTTGTGGA+AGG | + | chr4.4:20569141-20569160 | None:intergenic | 40.0% |
| !! | CTTTTTGTGAAAGACGGGTT+TGG | + | chr4.4:20569384-20569403 | None:intergenic | 40.0% |
| !!! | TTTTGGGTTCTGTGGGTAAT+GGG | - | chr4.4:20569629-20569648 | MS.gene35046:intron | 40.0% |
| !!! | TTTTTGGGTTCTGTGGGTAA+TGG | - | chr4.4:20569628-20569647 | MS.gene35046:intron | 40.0% |
| AAAGATTCCATCGCTGCTCA+GGG | - | chr4.4:20569314-20569333 | MS.gene35046:CDS | 45.0% | |
| CGCAAACAATCTGGTTATGG+TGG | - | chr4.4:20569353-20569372 | MS.gene35046:intron | 45.0% | |
| CGTTATGACCGCAAACAATC+TGG | - | chr4.4:20569344-20569363 | MS.gene35046:intron | 45.0% | |
| GACCGCAAACAATCTGGTTA+TGG | - | chr4.4:20569350-20569369 | MS.gene35046:intron | 45.0% | |
| GTGTTGTGGAAGGTAAGATG+AGG | + | chr4.4:20569131-20569150 | None:intergenic | 45.0% | |
| TAAAGATTCCATCGCTGCTC+AGG | - | chr4.4:20569313-20569332 | MS.gene35046:CDS | 45.0% | |
| !! | AGGTGCAAGCACTTTGAGAT+TGG | - | chr4.4:20569674-20569693 | MS.gene35046:intron | 45.0% |
| !! | GAGATTGGTGGTGACAAGAA+GGG | - | chr4.4:20569689-20569708 | MS.gene35046:intron | 45.0% |
| !! | TGAGATTGGTGGTGACAAGA+AGG | - | chr4.4:20569688-20569707 | MS.gene35046:intron | 45.0% |
| !! | TGCAAGCACTTTGAGATTGG+TGG | - | chr4.4:20569677-20569696 | MS.gene35046:intron | 45.0% |
| GGTGGTGACAAGAAGGGAAA+AGG | - | chr4.4:20569695-20569714 | MS.gene35046:intron | 50.0% | |
| GTGTTGAGGCTTCAGTGTCA+AGG | - | chr4.4:20569528-20569547 | MS.gene35046:CDS | 50.0% | |
| !! | GCACTCCTTGCTTTTGCAGT+AGG | + | chr4.4:20569252-20569271 | None:intergenic | 50.0% |
| ! | ACGTTTTCCCTGAGCAGCGA+TGG | + | chr4.4:20569324-20569343 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 20569109 | 20569733 | 20569109 | ID=MS.gene35046 |
| chr4.4 | mRNA | 20569109 | 20569733 | 20569109 | ID=MS.gene35046.t1;Parent=MS.gene35046 |
| chr4.4 | exon | 20569731 | 20569733 | 20569731 | ID=MS.gene35046.t1.exon1;Parent=MS.gene35046.t1 |
| chr4.4 | CDS | 20569731 | 20569733 | 20569731 | ID=cds.MS.gene35046.t1;Parent=MS.gene35046.t1 |
| chr4.4 | exon | 20569439 | 20569618 | 20569439 | ID=MS.gene35046.t1.exon2;Parent=MS.gene35046.t1 |
| chr4.4 | CDS | 20569439 | 20569618 | 20569439 | ID=cds.MS.gene35046.t1;Parent=MS.gene35046.t1 |
| chr4.4 | exon | 20569261 | 20569335 | 20569261 | ID=MS.gene35046.t1.exon3;Parent=MS.gene35046.t1 |
| chr4.4 | CDS | 20569261 | 20569335 | 20569261 | ID=cds.MS.gene35046.t1;Parent=MS.gene35046.t1 |
| chr4.4 | exon | 20569109 | 20569168 | 20569109 | ID=MS.gene35046.t1.exon4;Parent=MS.gene35046.t1 |
| chr4.4 | CDS | 20569109 | 20569168 | 20569109 | ID=cds.MS.gene35046.t1;Parent=MS.gene35046.t1 |
| Gene Sequence |
| Protein sequence |
