Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71613.t1 | XP_024626745.1 | 85 | 213 | 32 | 0 | 5 | 217 | 58 | 270 | 1.40E-72 | 282.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71613.t1 | I3SPL3 | 83.4 | 205 | 34 | 0 | 13 | 217 | 57 | 261 | 1.1e-66 | 262.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049666 | MS.gene71613 | 0.800539 | 1.37E-48 | -1.69E-46 |
| MS.gene051207 | MS.gene71613 | 0.800545 | 1.37E-48 | -1.69E-46 |
| MS.gene051594 | MS.gene71613 | 0.804109 | 2.53E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene71613 | 0.802235 | 6.18E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene71613 | 0.805097 | 1.57E-49 | -1.69E-46 |
| MS.gene053054 | MS.gene71613 | -0.804867 | 1.76E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene71613 | 0.829089 | 6.26E-55 | -1.69E-46 |
| MS.gene05365 | MS.gene71613 | 0.817073 | 3.98E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene71613 | 0.812723 | 3.67E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene71613 | 0.809015 | 2.33E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71613.t1 | MTR_1g106210 | 99.029 | 206 | 2 | 0 | 12 | 217 | 56 | 261 | 3.16e-148 | 413 |
| MS.gene71613.t1 | MTR_5g005040 | 39.333 | 150 | 63 | 5 | 65 | 212 | 109 | 232 | 6.51e-19 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71613.t1 | AT3G19650 | 37.805 | 164 | 89 | 6 | 56 | 217 | 150 | 302 | 8.58e-12 | 63.5 |
Find 53 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCCTTTCATTCCTTTC+TGG | 0.052010 | 1.4:-82201619 | None:intergenic |
| ATCTGATCATCCTCATTCTT+TGG | 0.243825 | 1.4:-82201889 | None:intergenic |
| TGAGGAAAAGGCCAGCATTT+AGG | 0.247990 | 1.4:+82201515 | MS.gene71613:CDS |
| TTTCTGGAGGGTGGCTAGTA+TGG | 0.266311 | 1.4:-82201603 | None:intergenic |
| TGAGAAATCACTGGCTAAAC+TGG | 0.294321 | 1.4:+82201432 | MS.gene71613:CDS |
| GAGGAAAAGGCCAGCATTTA+GGG | 0.296936 | 1.4:+82201516 | MS.gene71613:CDS |
| TTCTCTGTTTGAGTCTTAAT+AGG | 0.302168 | 1.4:-82201823 | None:intergenic |
| CTTCCTAGGTTCTCGCTTGT+TGG | 0.303209 | 1.4:-82201707 | None:intergenic |
| AGAGGAAGAGACAAATTTAA+TGG | 0.334230 | 1.4:+82201784 | MS.gene71613:CDS |
| TTTGACCTGTCTCCAACCTT+TGG | 0.360011 | 1.4:-82201393 | None:intergenic |
| TCCTCTGCAATTCGCTTCTC+AGG | 0.377354 | 1.4:-82201676 | None:intergenic |
| GCTTCTCAGGTCTATCTAGA+TGG | 0.381632 | 1.4:-82201663 | None:intergenic |
| TCAGGTCTATCTAGATGGTT+AGG | 0.410188 | 1.4:-82201658 | None:intergenic |
| GATGAAAATCCATCCTTCCT+AGG | 0.435435 | 1.4:-82201721 | None:intergenic |
| TCCTTTCATTCCTTTCTGGA+GGG | 0.447281 | 1.4:-82201615 | None:intergenic |
| GGATGATCAGATTGCAAAGC+TGG | 0.457045 | 1.4:+82201900 | MS.gene71613:CDS |
| TTCCTTTCATTCCTTTCTGG+AGG | 0.457901 | 1.4:-82201616 | None:intergenic |
| CAACACCTTCTGTGAGGAAA+AGG | 0.461132 | 1.4:+82201503 | MS.gene71613:CDS |
| TTTACTGTCACTGTCGCAGC+TGG | 0.462931 | 1.4:-82201574 | None:intergenic |
| ATCTTCTTCTCCCTAAATGC+TGG | 0.485997 | 1.4:-82201526 | None:intergenic |
| AAAGGTATATGCTCAAAGTA+AGG | 0.495225 | 1.4:+82201363 | MS.gene71613:CDS |
| TTTCATTCCTTTCTGGAGGG+TGG | 0.506208 | 1.4:-82201612 | None:intergenic |
| TTTCATCAAGAGTAAGGTAT+GGG | 0.512420 | 1.4:+82201737 | MS.gene71613:CDS |
| TGGTCGAAGCACTGGTCAAA+GGG | 0.514223 | 1.4:+82201214 | MS.gene71613:CDS |
| GTGCAGGTTGGTCGAAGCAC+TGG | 0.521659 | 1.4:+82201206 | MS.gene71613:CDS |
| AGAAGCGAATTGCAGAGGAC+AGG | 0.525004 | 1.4:+82201680 | MS.gene71613:CDS |
| TAAGGAGAATAACCAAAGGT+TGG | 0.542846 | 1.4:+82201381 | MS.gene71613:CDS |
| AATAACCAAAGGTTGGAGAC+AGG | 0.542889 | 1.4:+82201388 | MS.gene71613:CDS |
| TTGGTCGAAGCACTGGTCAA+AGG | 0.551358 | 1.4:+82201213 | MS.gene71613:CDS |
| GAAGCGAATTGCAGAGGACA+GGG | 0.551739 | 1.4:+82201681 | MS.gene71613:CDS |
| TTAAGACTCAAACAGAGAAG+TGG | 0.554278 | 1.4:+82201827 | MS.gene71613:CDS |
| GCAAAGAGATGAGAAATCAC+TGG | 0.563837 | 1.4:+82201423 | MS.gene71613:CDS |
| AAGCGAGAACCTAGGAAGGA+TGG | 0.574352 | 1.4:+82201712 | MS.gene71613:CDS |
| ACCCTCCAGAAAGGAATGAA+AGG | 0.576042 | 1.4:+82201614 | MS.gene71613:CDS |
| TCCAGAAAGGAATGAAAGGA+AGG | 0.580588 | 1.4:+82201618 | MS.gene71613:CDS |
| ATACTAGCCACCCTCCAGAA+AGG | 0.585699 | 1.4:+82201605 | MS.gene71613:CDS |
| GAAACATGATTTGTATCAAG+AGG | 0.586742 | 1.4:+82201849 | MS.gene71613:CDS |
| AAATCACTGGCTAAACTGGA+TGG | 0.588753 | 1.4:+82201436 | MS.gene71613:CDS |
| AGATTCTATTCCAAAGAATG+AGG | 0.590814 | 1.4:+82201879 | MS.gene71613:CDS |
| ACCTGAGAAGCGAATTGCAG+AGG | 0.607322 | 1.4:+82201675 | MS.gene71613:CDS |
| TGAATGGGGCAATACTGTGC+AGG | 0.607456 | 1.4:+82201190 | MS.gene71613:CDS |
| TTCATCAAGAGTAAGGTATG+GGG | 0.620262 | 1.4:+82201738 | MS.gene71613:CDS |
| CAACAAGCGAGAACCTAGGA+AGG | 0.622024 | 1.4:+82201708 | MS.gene71613:CDS |
| GATCTCAGTGGATTCAAGAG+AGG | 0.625358 | 1.4:+82201546 | MS.gene71613:CDS |
| AGAAAGGAATGAAAGGAAGG+AGG | 0.636511 | 1.4:+82201621 | MS.gene71613:CDS |
| TCATCAAGAGTAAGGTATGG+GGG | 0.637056 | 1.4:+82201739 | MS.gene71613:CDS |
| AATCCAACAAGCGAGAACCT+AGG | 0.638185 | 1.4:+82201704 | MS.gene71613:CDS |
| ACTGGTCAAAGGGAAGCTGA+TGG | 0.653810 | 1.4:+82201224 | MS.gene71613:CDS |
| TGGGGCAATACTGTGCAGGT+TGG | 0.672427 | 1.4:+82201194 | MS.gene71613:CDS |
| AAAGTAAGGAGAATAACCAA+AGG | 0.679155 | 1.4:+82201377 | MS.gene71613:CDS |
| ATGATCCAACACCTTCTGTG+AGG | 0.687855 | 1.4:+82201497 | MS.gene71613:CDS |
| TAGGGAGAAGAAGATCTCAG+TGG | 0.726006 | 1.4:+82201534 | MS.gene71613:CDS |
| GGAGGCAATAGCAATCACAG+AGG | 0.738956 | 1.4:+82201766 | MS.gene71613:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATATATATTATTTTCTTT+TGG | + | chr1.4:82201268-82201287 | MS.gene71613:intron | 10.0% |
| ! | TTTATTGTATATGGATGGTT+TGG | + | chr1.4:82201292-82201311 | MS.gene71613:intron | 25.0% |
| !!! | TCTTTTGGCTTTATTGTATA+TGG | + | chr1.4:82201283-82201302 | MS.gene71613:intron | 25.0% |
| !!! | TGTATGCAAACTATTTTTCT+GGG | + | chr1.4:82201091-82201110 | MS.gene71613:intron | 25.0% |
| AAAGTAAGGAGAATAACCAA+AGG | + | chr1.4:82201377-82201396 | MS.gene71613:CDS | 30.0% | |
| AGAGGAAGAGACAAATTTAA+TGG | + | chr1.4:82201784-82201803 | MS.gene71613:CDS | 30.0% | |
| AGATTCTATTCCAAAGAATG+AGG | + | chr1.4:82201879-82201898 | MS.gene71613:CDS | 30.0% | |
| TTCTCTGTTTGAGTCTTAAT+AGG | - | chr1.4:82201826-82201845 | None:intergenic | 30.0% | |
| TTTCATCAAGAGTAAGGTAT+GGG | + | chr1.4:82201737-82201756 | MS.gene71613:CDS | 30.0% | |
| ! | AAAGGTATATGCTCAAAGTA+AGG | + | chr1.4:82201363-82201382 | MS.gene71613:CDS | 30.0% |
| ! | ATGGATTTTCATCAAGAGTA+AGG | + | chr1.4:82201731-82201750 | MS.gene71613:CDS | 30.0% |
| ! | GAAACATGATTTGTATCAAG+AGG | + | chr1.4:82201849-82201868 | MS.gene71613:CDS | 30.0% |
| ! | TTTTAACAGCATCATGAATG+GGG | + | chr1.4:82201176-82201195 | MS.gene71613:intron | 30.0% |
| ! | TTTTCATCAAGAGTAAGGTA+TGG | + | chr1.4:82201736-82201755 | MS.gene71613:CDS | 30.0% |
| !! | GTATGCAAACTATTTTTCTG+GGG | + | chr1.4:82201092-82201111 | MS.gene71613:intron | 30.0% |
| !! | GTGTATGCAAACTATTTTTC+TGG | + | chr1.4:82201090-82201109 | MS.gene71613:intron | 30.0% |
| !! | GTTTGGAATCGAAGAATTTT+GGG | - | chr1.4:82201340-82201359 | None:intergenic | 30.0% |
| !! | GTTTTAACAGCATCATGAAT+GGG | + | chr1.4:82201175-82201194 | MS.gene71613:intron | 30.0% |
| !! | TGTTTGGAATCGAAGAATTT+TGG | - | chr1.4:82201341-82201360 | None:intergenic | 30.0% |
| !! | TTGGCTTTATTGTATATGGA+TGG | + | chr1.4:82201287-82201306 | MS.gene71613:intron | 30.0% |
| !!! | TGTTTTAACAGCATCATGAA+TGG | + | chr1.4:82201174-82201193 | MS.gene71613:intron | 30.0% |
| ATCTGATCATCCTCATTCTT+TGG | - | chr1.4:82201892-82201911 | None:intergenic | 35.0% | |
| GATGCTGTTAAAACATGCAT+TGG | - | chr1.4:82201169-82201188 | None:intergenic | 35.0% | |
| TAAGGAGAATAACCAAAGGT+TGG | + | chr1.4:82201381-82201400 | MS.gene71613:CDS | 35.0% | |
| TCTTCGATTCCAAACAGAAA+AGG | + | chr1.4:82201345-82201364 | MS.gene71613:intron | 35.0% | |
| TTAAGACTCAAACAGAGAAG+TGG | + | chr1.4:82201827-82201846 | MS.gene71613:CDS | 35.0% | |
| TTCATCAAGAGTAAGGTATG+GGG | + | chr1.4:82201738-82201757 | MS.gene71613:CDS | 35.0% | |
| ! | GAGCATATACCTTTTCTGTT+TGG | - | chr1.4:82201357-82201376 | None:intergenic | 35.0% |
| AATAACCAAAGGTTGGAGAC+AGG | + | chr1.4:82201388-82201407 | MS.gene71613:CDS | 40.0% | |
| AGAAAGGAATGAAAGGAAGG+AGG | + | chr1.4:82201621-82201640 | MS.gene71613:CDS | 40.0% | |
| ATCTTCTTCTCCCTAAATGC+TGG | - | chr1.4:82201529-82201548 | None:intergenic | 40.0% | |
| GATGAAAATCCATCCTTCCT+AGG | - | chr1.4:82201724-82201743 | None:intergenic | 40.0% | |
| GCAAAGAGATGAGAAATCAC+TGG | + | chr1.4:82201423-82201442 | MS.gene71613:CDS | 40.0% | |
| TCAGGTCTATCTAGATGGTT+AGG | - | chr1.4:82201661-82201680 | None:intergenic | 40.0% | |
| TCATCAAGAGTAAGGTATGG+GGG | + | chr1.4:82201739-82201758 | MS.gene71613:CDS | 40.0% | |
| TCCAGAAAGGAATGAAAGGA+AGG | + | chr1.4:82201618-82201637 | MS.gene71613:CDS | 40.0% | |
| TCCTTCCTTTCATTCCTTTC+TGG | - | chr1.4:82201622-82201641 | None:intergenic | 40.0% | |
| TCCTTTCATTCCTTTCTGGA+GGG | - | chr1.4:82201618-82201637 | None:intergenic | 40.0% | |
| TTCCTTTCATTCCTTTCTGG+AGG | - | chr1.4:82201619-82201638 | None:intergenic | 40.0% | |
| ! | AAATCACTGGCTAAACTGGA+TGG | + | chr1.4:82201436-82201455 | MS.gene71613:CDS | 40.0% |
| ! | TGAGAAATCACTGGCTAAAC+TGG | + | chr1.4:82201432-82201451 | MS.gene71613:CDS | 40.0% |
| !! | GAAGAGACAGTTTTCGAGAA+AGG | + | chr1.4:82201470-82201489 | MS.gene71613:CDS | 40.0% |
| !!! | AAACTATTTTTCTGGGGCGT+GGG | + | chr1.4:82201098-82201117 | MS.gene71613:intron | 40.0% |
| AATCCAACAAGCGAGAACCT+AGG | + | chr1.4:82201704-82201723 | MS.gene71613:CDS | 45.0% | |
| ACCCTCCAGAAAGGAATGAA+AGG | + | chr1.4:82201614-82201633 | MS.gene71613:CDS | 45.0% | |
| ATGATCCAACACCTTCTGTG+AGG | + | chr1.4:82201497-82201516 | MS.gene71613:CDS | 45.0% | |
| CAACACCTTCTGTGAGGAAA+AGG | + | chr1.4:82201503-82201522 | MS.gene71613:CDS | 45.0% | |
| GAGGAAAAGGCCAGCATTTA+GGG | + | chr1.4:82201516-82201535 | MS.gene71613:CDS | 45.0% | |
| GCTTCTCAGGTCTATCTAGA+TGG | - | chr1.4:82201666-82201685 | None:intergenic | 45.0% | |
| GGATGATCAGATTGCAAAGC+TGG | + | chr1.4:82201900-82201919 | MS.gene71613:CDS | 45.0% | |
| TAGGGAGAAGAAGATCTCAG+TGG | + | chr1.4:82201534-82201553 | MS.gene71613:CDS | 45.0% | |
| TGAGGAAAAGGCCAGCATTT+AGG | + | chr1.4:82201515-82201534 | MS.gene71613:CDS | 45.0% | |
| TTTGACCTGTCTCCAACCTT+TGG | - | chr1.4:82201396-82201415 | None:intergenic | 45.0% | |
| ! | GATCTCAGTGGATTCAAGAG+AGG | + | chr1.4:82201546-82201565 | MS.gene71613:CDS | 45.0% |
| ! | GCATTGGAAAGCATATACCG+AGG | - | chr1.4:82201153-82201172 | None:intergenic | 45.0% |
| ! | TTTCATTCCTTTCTGGAGGG+TGG | - | chr1.4:82201615-82201634 | None:intergenic | 45.0% |
| !! | CTTTTCCTCACAGAAGGTGT+TGG | - | chr1.4:82201505-82201524 | None:intergenic | 45.0% |
| !!! | CAAACTATTTTTCTGGGGCG+TGG | + | chr1.4:82201097-82201116 | MS.gene71613:intron | 45.0% |
| !! | TATATATCAATATAAAATTA+AGG | - | chr1.4:82201257-82201276 | None:intergenic | 5.0% |
| AAGCGAGAACCTAGGAAGGA+TGG | + | chr1.4:82201712-82201731 | MS.gene71613:CDS | 50.0% | |
| ACCTGAGAAGCGAATTGCAG+AGG | + | chr1.4:82201675-82201694 | MS.gene71613:CDS | 50.0% | |
| ACTGGTCAAAGGGAAGCTGA+TGG | + | chr1.4:82201224-82201243 | MS.gene71613:CDS | 50.0% | |
| AGAAGCGAATTGCAGAGGAC+AGG | + | chr1.4:82201680-82201699 | MS.gene71613:CDS | 50.0% | |
| AGAGTAAGGTATGGGGGAAG+TGG | + | chr1.4:82201745-82201764 | MS.gene71613:CDS | 50.0% | |
| ATACTAGCCACCCTCCAGAA+AGG | + | chr1.4:82201605-82201624 | MS.gene71613:CDS | 50.0% | |
| CAACAAGCGAGAACCTAGGA+AGG | + | chr1.4:82201708-82201727 | MS.gene71613:CDS | 50.0% | |
| CTTCCTAGGTTCTCGCTTGT+TGG | - | chr1.4:82201710-82201729 | None:intergenic | 50.0% | |
| GAAGCGAATTGCAGAGGACA+GGG | + | chr1.4:82201681-82201700 | MS.gene71613:CDS | 50.0% | |
| GGAGGCAATAGCAATCACAG+AGG | + | chr1.4:82201766-82201785 | MS.gene71613:CDS | 50.0% | |
| TCCTCTGCAATTCGCTTCTC+AGG | - | chr1.4:82201679-82201698 | None:intergenic | 50.0% | |
| TGAATGGGGCAATACTGTGC+AGG | + | chr1.4:82201190-82201209 | MS.gene71613:CDS | 50.0% | |
| TTTACTGTCACTGTCGCAGC+TGG | - | chr1.4:82201577-82201596 | None:intergenic | 50.0% | |
| ! | GCATATACCGAGGCACTTGA+AGG | - | chr1.4:82201143-82201162 | None:intergenic | 50.0% |
| ! | TTTCTGGAGGGTGGCTAGTA+TGG | - | chr1.4:82201606-82201625 | None:intergenic | 50.0% |
| !! | TGGTCGAAGCACTGGTCAAA+GGG | + | chr1.4:82201214-82201233 | MS.gene71613:CDS | 50.0% |
| !! | TTGGTCGAAGCACTGGTCAA+AGG | + | chr1.4:82201213-82201232 | MS.gene71613:CDS | 50.0% |
| GTAAGGTATGGGGGAAGTGG+AGG | + | chr1.4:82201748-82201767 | MS.gene71613:CDS | 55.0% | |
| TGGGGCAATACTGTGCAGGT+TGG | + | chr1.4:82201194-82201213 | MS.gene71613:CDS | 55.0% | |
| ! | ACCGAGGCACTTGAAGGCAT+GGG | - | chr1.4:82201137-82201156 | None:intergenic | 55.0% |
| ! | TACCGAGGCACTTGAAGGCA+TGG | - | chr1.4:82201138-82201157 | None:intergenic | 55.0% |
| !!! | GCTGGCCTTTTCCTCACAGA+AGG | - | chr1.4:82201511-82201530 | None:intergenic | 55.0% |
| CCCCATGCCTTCAAGTGCCT+CGG | + | chr1.4:82201133-82201152 | MS.gene71613:intron | 60.0% | |
| ! | CCGAGGCACTTGAAGGCATG+GGG | - | chr1.4:82201136-82201155 | None:intergenic | 60.0% |
| !! | GTGCAGGTTGGTCGAAGCAC+TGG | + | chr1.4:82201206-82201225 | MS.gene71613:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 82201080 | 82201942 | 82201080 | ID=MS.gene71613 |
| chr1.4 | mRNA | 82201080 | 82201942 | 82201080 | ID=MS.gene71613.t1;Parent=MS.gene71613 |
| chr1.4 | exon | 82201080 | 82201091 | 82201080 | ID=MS.gene71613.t1.exon1;Parent=MS.gene71613.t1 |
| chr1.4 | CDS | 82201080 | 82201091 | 82201080 | ID=cds.MS.gene71613.t1;Parent=MS.gene71613.t1 |
| chr1.4 | exon | 82201185 | 82201245 | 82201185 | ID=MS.gene71613.t1.exon2;Parent=MS.gene71613.t1 |
| chr1.4 | CDS | 82201185 | 82201245 | 82201185 | ID=cds.MS.gene71613.t1;Parent=MS.gene71613.t1 |
| chr1.4 | exon | 82201362 | 82201942 | 82201362 | ID=MS.gene71613.t1.exon3;Parent=MS.gene71613.t1 |
| chr1.4 | CDS | 82201362 | 82201942 | 82201362 | ID=cds.MS.gene71613.t1;Parent=MS.gene71613.t1 |
| Gene Sequence |
| Protein sequence |
