Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71628.t1 | XP_003610623.1 | 100 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 1.40E-27 | 131.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71628.t1 | Q680P8 | 92.9 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 4.4e-27 | 120.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71628.t1 | G7K0X3 | 100.0 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.8e-28 | 131.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene71628 | MS.gene064836 | PPI |
| MS.gene71628 | MS.gene046945 | PPI |
| MS.gene71628 | MS.gene69327 | PPI |
| MS.gene71628 | MS.gene40529 | PPI |
| MS.gene71628 | MS.gene52771 | PPI |
| MS.gene046945 | MS.gene71628 | PPI |
| MS.gene71628 | MS.gene024965 | PPI |
| MS.gene71628 | MS.gene014345 | PPI |
| MS.gene71628 | MS.gene060279 | PPI |
| MS.gene71628 | MS.gene92361 | PPI |
| MS.gene71628 | MS.gene91297 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71628.t1 | MTR_5g005130 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| MS.gene71628.t1 | MTR_3g115330 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| MS.gene71628.t1 | MTR_1g106755 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71628.t1 | AT4G33865 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene71628.t1 | AT3G44010 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene71628.t1 | AT3G43980 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene71628.t1 | AT3G44010 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
Find 25 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGGAGATTGGCTTCATTA+AGG | 0.318951 | 1.4:-82373043 | MS.gene71628:intron |
| CACAGCAATGCCAAGGAGAT+TGG | 0.355374 | 1.4:-82373054 | MS.gene71628:CDS |
| CATGGATTGATAAGGAAGTA+TGG | 0.378864 | 1.4:-82373102 | MS.gene71628:CDS |
| CCATGGGACACTCAAATGTT+TGG | 0.382308 | 1.4:-82373758 | MS.gene71628:CDS |
| GAACCAGGACCATAGGTCTT+GGG | 0.410602 | 1.4:+82373726 | None:intergenic |
| AGCATTGCCTGCAGCACATA+AGG | 0.453112 | 1.4:+82373079 | None:intergenic |
| GGAACCAGGACCATAGGTCT+TGG | 0.464100 | 1.4:+82373725 | None:intergenic |
| ATGGTCCTGGTTCCCGCACT+TGG | 0.475887 | 1.4:-82373716 | MS.gene71628:intron |
| CTTAATGAAGCCAATCTCCT+TGG | 0.487748 | 1.4:+82373044 | None:intergenic |
| CACCCCAAGACCTATGGTCC+TGG | 0.491604 | 1.4:-82373729 | MS.gene71628:CDS |
| AACTCTCACCCCAAGACCTA+TGG | 0.505813 | 1.4:-82373735 | MS.gene71628:CDS |
| GGAATTCACATGGATTGATA+AGG | 0.515737 | 1.4:-82373110 | MS.gene71628:CDS |
| ATATACCAAGTGCGGGAACC+AGG | 0.531737 | 1.4:+82373711 | None:intergenic |
| AGTATGGCCTTATGTGCTGC+AGG | 0.540472 | 1.4:-82373086 | MS.gene71628:CDS |
| CCAAACATTTGAGTGTCCCA+TGG | 0.543494 | 1.4:+82373758 | None:intergenic |
| TAACTTGTAGCCGTGTGTGC+GGG | 0.573381 | 1.4:-82373131 | MS.gene71628:intron |
| CATGTGAATTCCCGCACACA+CGG | 0.574250 | 1.4:+82373121 | None:intergenic |
| AAGAATGAATATACCAAGTG+CGG | 0.579658 | 1.4:+82373703 | None:intergenic |
| AGTGCGGGAACCAGGACCAT+AGG | 0.585453 | 1.4:+82373719 | None:intergenic |
| AACCAGGACCATAGGTCTTG+GGG | 0.597207 | 1.4:+82373727 | None:intergenic |
| CGTGTGTGCGGGAATTCACA+TGG | 0.601988 | 1.4:-82373120 | MS.gene71628:CDS |
| AGAATGAATATACCAAGTGC+GGG | 0.603783 | 1.4:+82373704 | None:intergenic |
| ATGCTTCCACAGCAATGCCA+AGG | 0.627314 | 1.4:-82373061 | MS.gene71628:CDS |
| CAATCTCCTTGGCATTGCTG+TGG | 0.640523 | 1.4:+82373055 | None:intergenic |
| ATAACTTGTAGCCGTGTGTG+CGG | 0.665136 | 1.4:-82373132 | MS.gene71628:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTATTGATAATATGTTTT+GGG | - | chr1.4:82373219-82373238 | MS.gene71628:intron | 15.0% |
| !!! | TTATGTTATTGCTATTATAT+CGG | - | chr1.4:82373406-82373425 | MS.gene71628:intron | 15.0% |
| !! | AAACCTAAATGAATTGAAAA+AGG | + | chr1.4:82373048-82373067 | None:intergenic | 20.0% |
| !! | GATATAATAGCAATAACATA+AGG | + | chr1.4:82373407-82373426 | None:intergenic | 20.0% |
| !! | GATTCAAAATTCATAAAAGA+GGG | + | chr1.4:82373118-82373137 | None:intergenic | 20.0% |
| !! | GCAACAAAAATAAAAACAAA+AGG | + | chr1.4:82373748-82373767 | None:intergenic | 20.0% |
| !! | TAATTAAGATTTGATTCAGA+TGG | - | chr1.4:82373301-82373320 | MS.gene71628:intron | 20.0% |
| !!! | ATTTAGGTTTCTAGTATTTT+GGG | - | chr1.4:82373058-82373077 | MS.gene71628:CDS | 20.0% |
| !!! | GTGTTATTGATAATATGTTT+TGG | - | chr1.4:82373218-82373237 | MS.gene71628:intron | 20.0% |
| !!! | GTTATTGATAATATGTTTTG+GGG | - | chr1.4:82373220-82373239 | MS.gene71628:intron | 20.0% |
| !!! | TAACCTTTTTCAATTCATTT+AGG | - | chr1.4:82373042-82373061 | MS.gene71628:intron | 20.0% |
| !!! | TTGGGGATTTTTATTTTTTT+GGG | - | chr1.4:82373237-82373256 | MS.gene71628:intron | 20.0% |
| !!! | TTTGGGGATTTTTATTTTTT+TGG | - | chr1.4:82373236-82373255 | MS.gene71628:intron | 20.0% |
| ! | CAAAATGTAAACTTTAACAG+CGG | + | chr1.4:82373093-82373112 | None:intergenic | 25.0% |
| ! | CGATTCAAAATTCATAAAAG+AGG | + | chr1.4:82373119-82373138 | None:intergenic | 25.0% |
| ! | TTTGAACATATGTAGTTTAG+TGG | - | chr1.4:82373377-82373396 | MS.gene71628:intron | 25.0% |
| !! | ATTTTACATGATTGTATCTG+AGG | + | chr1.4:82373709-82373728 | None:intergenic | 25.0% |
| !! | TAATGGTTTTCTATTGCTTT+GGG | - | chr1.4:82373164-82373183 | MS.gene71628:intron | 25.0% |
| !!! | CATTTAGGTTTCTAGTATTT+TGG | - | chr1.4:82373057-82373076 | MS.gene71628:CDS | 25.0% |
| AAGAATGAATATACCAAGTG+CGG | + | chr1.4:82373006-82373025 | None:intergenic | 30.0% | |
| ! | CTAATGGTTTTCTATTGCTT+TGG | - | chr1.4:82373163-82373182 | MS.gene71628:intron | 30.0% |
| !! | GTGTTGTTTCTATGTTCTAA+TGG | - | chr1.4:82373147-82373166 | MS.gene71628:intron | 30.0% |
| AGAATGAATATACCAAGTGC+GGG | + | chr1.4:82373005-82373024 | None:intergenic | 35.0% | |
| TGCTATTATATCGGTACTAG+AGG | - | chr1.4:82373415-82373434 | MS.gene71628:intron | 35.0% | |
| ! | CATGGATTGATAAGGAAGTA+TGG | - | chr1.4:82373604-82373623 | MS.gene71628:intron | 35.0% |
| ! | GGAATTCACATGGATTGATA+AGG | - | chr1.4:82373596-82373615 | MS.gene71628:intron | 35.0% |
| CTTAATGAAGCCAATCTCCT+TGG | + | chr1.4:82373665-82373684 | None:intergenic | 40.0% | |
| !! | CAAGGAGATTGGCTTCATTA+AGG | - | chr1.4:82373663-82373682 | MS.gene71628:intron | 40.0% |
| !! | TGCTTTGGGTTGTACTGTTA+TGG | - | chr1.4:82373178-82373197 | MS.gene71628:intron | 40.0% |
| ! | ATAACTTGTAGCCGTGTGTG+CGG | - | chr1.4:82373574-82373593 | MS.gene71628:intron | 45.0% |
| AACCAGGACCATAGGTCTTG+GGG | + | chr1.4:82372982-82373001 | None:intergenic | 50.0% | |
| AACTCTCACCCCAAGACCTA+TGG | - | chr1.4:82372971-82372990 | MS.gene71628:intron | 50.0% | |
| AGCATTGCCTGCAGCACATA+AGG | + | chr1.4:82373630-82373649 | None:intergenic | 50.0% | |
| ATATACCAAGTGCGGGAACC+AGG | + | chr1.4:82372998-82373017 | None:intergenic | 50.0% | |
| ATGCTTCCACAGCAATGCCA+AGG | - | chr1.4:82373645-82373664 | MS.gene71628:intron | 50.0% | |
| CAATCTCCTTGGCATTGCTG+TGG | + | chr1.4:82373654-82373673 | None:intergenic | 50.0% | |
| CACAGCAATGCCAAGGAGAT+TGG | - | chr1.4:82373652-82373671 | MS.gene71628:intron | 50.0% | |
| CATGTGAATTCCCGCACACA+CGG | + | chr1.4:82373588-82373607 | None:intergenic | 50.0% | |
| GAACCAGGACCATAGGTCTT+GGG | + | chr1.4:82372983-82373002 | None:intergenic | 50.0% | |
| ! | TAACTTGTAGCCGTGTGTGC+GGG | - | chr1.4:82373575-82373594 | MS.gene71628:intron | 50.0% |
| !! | AGTATGGCCTTATGTGCTGC+AGG | - | chr1.4:82373620-82373639 | MS.gene71628:intron | 50.0% |
| CGTGTGTGCGGGAATTCACA+TGG | - | chr1.4:82373586-82373605 | MS.gene71628:intron | 55.0% | |
| GGAACCAGGACCATAGGTCT+TGG | + | chr1.4:82372984-82373003 | None:intergenic | 55.0% | |
| AGTGCGGGAACCAGGACCAT+AGG | + | chr1.4:82372990-82373009 | None:intergenic | 60.0% | |
| ATGGTCCTGGTTCCCGCACT+TGG | - | chr1.4:82372990-82373009 | MS.gene71628:intron | 60.0% | |
| CACCCCAAGACCTATGGTCC+TGG | - | chr1.4:82372977-82372996 | MS.gene71628:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 82372950 | 82373778 | 82372950 | ID=MS.gene71628 |
| chr1.4 | mRNA | 82372950 | 82373778 | 82372950 | ID=MS.gene71628.t1;Parent=MS.gene71628 |
| chr1.4 | exon | 82373717 | 82373778 | 82373717 | ID=MS.gene71628.t1.exon1;Parent=MS.gene71628.t1 |
| chr1.4 | CDS | 82373717 | 82373778 | 82373717 | ID=cds.MS.gene71628.t1;Parent=MS.gene71628.t1 |
| chr1.4 | exon | 82373044 | 82373143 | 82373044 | ID=MS.gene71628.t1.exon2;Parent=MS.gene71628.t1 |
| chr1.4 | CDS | 82373044 | 82373143 | 82373044 | ID=cds.MS.gene71628.t1;Parent=MS.gene71628.t1 |
| chr1.4 | exon | 82372950 | 82372958 | 82372950 | ID=MS.gene71628.t1.exon3;Parent=MS.gene71628.t1 |
| chr1.4 | CDS | 82372950 | 82372958 | 82372950 | ID=cds.MS.gene71628.t1;Parent=MS.gene71628.t1 |
| Gene Sequence |
| Protein sequence |
