Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064836.t1 | XP_003610623.1 | 100 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 1.40E-27 | 131.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064836.t1 | Q680P8 | 92.9 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 4.4e-27 | 120.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064836.t1 | G7K0X3 | 100.0 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.8e-28 | 131.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057237 | MS.gene064836 | 0.830962 | 2.19E-55 | -1.69E-46 |
| MS.gene060279 | MS.gene064836 | 0.829098 | 6.22E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene060279 | MS.gene064836 | PPI |
| MS.gene71628 | MS.gene064836 | PPI |
| MS.gene046945 | MS.gene064836 | PPI |
| MS.gene064836 | MS.gene69327 | PPI |
| MS.gene064836 | MS.gene40529 | PPI |
| MS.gene014345 | MS.gene064836 | PPI |
| MS.gene064836 | MS.gene060279 | PPI |
| MS.gene064836 | MS.gene92361 | PPI |
| MS.gene064836 | MS.gene52771 | PPI |
| MS.gene064836 | MS.gene91297 | PPI |
| MS.gene40529 | MS.gene064836 | PPI |
| MS.gene024965 | MS.gene064836 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064836.t1 | MTR_5g005130 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| MS.gene064836.t1 | MTR_3g115330 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| MS.gene064836.t1 | MTR_1g106755 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064836.t1 | AT4G33865 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene064836.t1 | AT3G44010 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene064836.t1 | AT3G43980 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene064836.t1 | AT3G44010 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
Find 20 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGCAATGCCAAAGAAAT+TGG | 0.191911 | 3.4:+98657369 | MS.gene064836:CDS |
| CAAAGAAATTGGTTTCATTA+AGG | 0.240769 | 3.4:+98657380 | MS.gene064836:CDS |
| CTTAATGAAACCAATTTCTT+TGG | 0.274134 | 3.4:-98657379 | None:intergenic |
| CCATGGGTCACTCCAACGTT+TGG | 0.417313 | 3.4:+98656816 | None:intergenic |
| GAACCAGGACCATAGGTCTT+GGG | 0.430692 | 3.4:-98656848 | None:intergenic |
| AGAACCAGGACCATAGGTCT+TGG | 0.460388 | 3.4:-98656849 | None:intergenic |
| CACCCCAAGACCTATGGTCC+TGG | 0.491604 | 3.4:+98656845 | MS.gene064836:CDS |
| GCAATTCTCATGGATTGATC+AGG | 0.493067 | 3.4:+98657313 | MS.gene064836:CDS |
| AACTCTCACCCCAAGACCTA+TGG | 0.505813 | 3.4:+98656839 | MS.gene064836:CDS |
| TCATACCATGTGCGAGAACC+AGG | 0.516036 | 3.4:-98656863 | None:intergenic |
| AGTACGGACTCATGTGCTGC+AGG | 0.547263 | 3.4:+98657337 | MS.gene064836:CDS |
| CAATTTCTTTGGCATTGCTG+TGG | 0.550649 | 3.4:-98657368 | None:intergenic |
| ATGGTCCTGGTTCTCGCACA+TGG | 0.572066 | 3.4:+98656858 | MS.gene064836:CDS |
| CCAAACGTTGGAGTGACCCA+TGG | 0.572985 | 3.4:-98656816 | None:intergenic |
| CATGGATTGATCAGGAAGTA+CGG | 0.575110 | 3.4:+98657321 | MS.gene064836:CDS |
| CGTGTGTGTGGCAATTCTCA+TGG | 0.579493 | 3.4:+98657303 | MS.gene064836:CDS |
| TGTGCGAGAACCAGGACCAT+AGG | 0.585453 | 3.4:-98656855 | None:intergenic |
| AACCAGGACCATAGGTCTTG+GGG | 0.607026 | 3.4:-98656847 | None:intergenic |
| CATGAGAATTGCCACACACA+CGG | 0.620870 | 3.4:-98657302 | None:intergenic |
| GGGGTGAGAGTTCCAAACGT+TGG | 0.647246 | 3.4:-98656828 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CCTTAATTTGAAATTAAAAT+CGG | + | chr3.4:98657108-98657127 | MS.gene064836:intron | 15.0% |
| !!! | TTTCATTTTTAAAATTTCTG+AGG | + | chr3.4:98657002-98657021 | MS.gene064836:intron | 15.0% |
| !!! | CCGATTTTAATTTCAAATTA+AGG | - | chr3.4:98657111-98657130 | None:intergenic | 20.0% |
| ! | AAACACAGTAATCAGAAATA+GGG | - | chr3.4:98656949-98656968 | None:intergenic | 25.0% |
| ! | CTTAATGAAACCAATTTCTT+TGG | - | chr3.4:98657382-98657401 | None:intergenic | 25.0% |
| ! | TAAACACAGTAATCAGAAAT+AGG | - | chr3.4:98656950-98656969 | None:intergenic | 25.0% |
| !! | CAAAGAAATTGGTTTCATTA+AGG | + | chr3.4:98657380-98657399 | MS.gene064836:CDS | 25.0% |
| AGAAATCTAGACCAACAAAT+AGG | - | chr3.4:98657426-98657445 | None:intergenic | 30.0% | |
| ATACTGCAACACAGAAAAAA+GGG | - | chr3.4:98657489-98657508 | None:intergenic | 30.0% | |
| ATCGAAGATTCATTCTTCAA+GGG | - | chr3.4:98656926-98656945 | None:intergenic | 30.0% | |
| GATTACTGTGATTCATGATT+TGG | + | chr3.4:98657034-98657053 | MS.gene064836:intron | 30.0% | |
| TCAAGGGATGAAAGAAAAAA+AGG | - | chr3.4:98656910-98656929 | None:intergenic | 30.0% | |
| TCATGAATCACAGTAATCTA+TGG | - | chr3.4:98657032-98657051 | None:intergenic | 30.0% | |
| TGATGTACTTTCCTATTTGT+TGG | + | chr3.4:98657412-98657431 | MS.gene064836:intron | 30.0% | |
| ATATGACCTAGCGTTTAGAT+GGG | + | chr3.4:98656973-98656992 | MS.gene064836:intron | 35.0% | |
| GATACTGCAACACAGAAAAA+AGG | - | chr3.4:98657490-98657509 | None:intergenic | 35.0% | |
| GATCGAAGATTCATTCTTCA+AGG | - | chr3.4:98656927-98656946 | None:intergenic | 35.0% | |
| CAATTTCTTTGGCATTGCTG+TGG | - | chr3.4:98657371-98657390 | None:intergenic | 40.0% | |
| CACAGCAATGCCAAAGAAAT+TGG | + | chr3.4:98657369-98657388 | MS.gene064836:CDS | 40.0% | |
| CATATGACCTAGCGTTTAGA+TGG | + | chr3.4:98656972-98656991 | MS.gene064836:intron | 40.0% | |
| TTAAAACCGTCTCCAATGCT+AGG | + | chr3.4:98657077-98657096 | MS.gene064836:intron | 40.0% | |
| ! | CATGGATTGATCAGGAAGTA+CGG | + | chr3.4:98657321-98657340 | MS.gene064836:CDS | 40.0% |
| ! | GCAATTCTCATGGATTGATC+AGG | + | chr3.4:98657313-98657332 | MS.gene064836:CDS | 40.0% |
| ACACACACGGCTACAAAACA+CGG | - | chr3.4:98657292-98657311 | None:intergenic | 45.0% | |
| CATGAGAATTGCCACACACA+CGG | - | chr3.4:98657305-98657324 | None:intergenic | 45.0% | |
| TCGAATCCCATCTAAACGCT+AGG | - | chr3.4:98656982-98657001 | None:intergenic | 45.0% | |
| ! | AAGGCATCTTGACCTAGCAT+TGG | - | chr3.4:98657092-98657111 | None:intergenic | 45.0% |
| ! | TCTTGACCTAGCATTGGAGA+CGG | - | chr3.4:98657086-98657105 | None:intergenic | 45.0% |
| AACCAGGACCATAGGTCTTG+GGG | - | chr3.4:98656850-98656869 | None:intergenic | 50.0% | |
| AACTCTCACCCCAAGACCTA+TGG | + | chr3.4:98656839-98656858 | MS.gene064836:CDS | 50.0% | |
| AGAACCAGGACCATAGGTCT+TGG | - | chr3.4:98656852-98656871 | None:intergenic | 50.0% | |
| CGTGTGTGTGGCAATTCTCA+TGG | + | chr3.4:98657303-98657322 | MS.gene064836:CDS | 50.0% | |
| GAACCAGGACCATAGGTCTT+GGG | - | chr3.4:98656851-98656870 | None:intergenic | 50.0% | |
| TCATACCATGTGCGAGAACC+AGG | - | chr3.4:98656866-98656885 | None:intergenic | 50.0% | |
| ! | GTGTTTTGTAGCCGTGTGTG+TGG | + | chr3.4:98657291-98657310 | MS.gene064836:intron | 50.0% |
| AGTACGGACTCATGTGCTGC+AGG | + | chr3.4:98657337-98657356 | MS.gene064836:CDS | 55.0% | |
| GGGGTGAGAGTTCCAAACGT+TGG | - | chr3.4:98656831-98656850 | None:intergenic | 55.0% | |
| TGTGCGAGAACCAGGACCAT+AGG | - | chr3.4:98656858-98656877 | None:intergenic | 55.0% | |
| ! | ATGGTCCTGGTTCTCGCACA+TGG | + | chr3.4:98656858-98656877 | MS.gene064836:CDS | 55.0% |
| CACCCCAAGACCTATGGTCC+TGG | + | chr3.4:98656845-98656864 | MS.gene064836:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 98656818 | 98657514 | 98656818 | ID=MS.gene064836 |
| chr3.4 | mRNA | 98656818 | 98657514 | 98656818 | ID=MS.gene064836.t1;Parent=MS.gene064836 |
| chr3.4 | exon | 98656818 | 98656879 | 98656818 | ID=MS.gene064836.t1.exon1;Parent=MS.gene064836.t1 |
| chr3.4 | CDS | 98656818 | 98656879 | 98656818 | ID=cds.MS.gene064836.t1;Parent=MS.gene064836.t1 |
| chr3.4 | exon | 98657302 | 98657401 | 98657302 | ID=MS.gene064836.t1.exon2;Parent=MS.gene064836.t1 |
| chr3.4 | CDS | 98657302 | 98657401 | 98657302 | ID=cds.MS.gene064836.t1;Parent=MS.gene064836.t1 |
| chr3.4 | exon | 98657506 | 98657514 | 98657506 | ID=MS.gene064836.t1.exon3;Parent=MS.gene064836.t1 |
| chr3.4 | CDS | 98657506 | 98657514 | 98657506 | ID=cds.MS.gene064836.t1;Parent=MS.gene064836.t1 |
| Gene Sequence |
| Protein sequence |
