Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69327.t1 | XP_003610623.1 | 100 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 1.40E-27 | 131.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69327.t1 | Q680P8 | 92.9 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 4.4e-27 | 120.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69327.t1 | G7K0X3 | 100.0 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.8e-28 | 131.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057237 | MS.gene69327 | 0.832238 | 1.06E-55 | -1.69E-46 |
| MS.gene060279 | MS.gene69327 | 0.830894 | 2.27E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene046945 | MS.gene69327 | PPI |
| MS.gene40529 | MS.gene69327 | PPI |
| MS.gene024965 | MS.gene69327 | PPI |
| MS.gene69327 | MS.gene92361 | PPI |
| MS.gene064836 | MS.gene69327 | PPI |
| MS.gene014345 | MS.gene69327 | PPI |
| MS.gene91297 | MS.gene69327 | PPI |
| MS.gene71628 | MS.gene69327 | PPI |
| MS.gene52771 | MS.gene69327 | PPI |
| MS.gene060279 | MS.gene69327 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69327.t1 | MTR_5g005130 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| MS.gene69327.t1 | MTR_3g115330 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| MS.gene69327.t1 | MTR_1g106755 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69327.t1 | AT4G33865 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene69327.t1 | AT3G44010 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene69327.t1 | AT3G43980 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene69327.t1 | AT3G44010 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
Find 20 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGCAATGCCAAAGAAAT+TGG | 0.191911 | 3.3:+94056684 | MS.gene69327:CDS |
| CAAAGAAATTGGTTTCATTA+AGG | 0.240769 | 3.3:+94056695 | MS.gene69327:CDS |
| CTTAATGAAACCAATTTCTT+TGG | 0.274134 | 3.3:-94056694 | None:intergenic |
| CCATGGGTCACTCCAACGTT+TGG | 0.417313 | 3.3:+94056131 | None:intergenic |
| GAACCAGGACCATAGGTCTT+GGG | 0.430692 | 3.3:-94056163 | None:intergenic |
| AGAACCAGGACCATAGGTCT+TGG | 0.460388 | 3.3:-94056164 | None:intergenic |
| CACCCCAAGACCTATGGTCC+TGG | 0.491604 | 3.3:+94056160 | MS.gene69327:CDS |
| GCAATTCTCATGGATTGATC+AGG | 0.493067 | 3.3:+94056628 | MS.gene69327:CDS |
| AACTCTCACCCCAAGACCTA+TGG | 0.505813 | 3.3:+94056154 | MS.gene69327:CDS |
| TCATACCATGTGCGAGAACC+AGG | 0.516036 | 3.3:-94056178 | None:intergenic |
| AGTACGGACTCATGTGCTGC+AGG | 0.547263 | 3.3:+94056652 | MS.gene69327:CDS |
| CAATTTCTTTGGCATTGCTG+TGG | 0.550649 | 3.3:-94056683 | None:intergenic |
| ATGGTCCTGGTTCTCGCACA+TGG | 0.572066 | 3.3:+94056173 | MS.gene69327:CDS |
| CCAAACGTTGGAGTGACCCA+TGG | 0.572985 | 3.3:-94056131 | None:intergenic |
| CATGGATTGATCAGGAAGTA+CGG | 0.575110 | 3.3:+94056636 | MS.gene69327:CDS |
| CGTGTGTGTGGCAATTCTCA+TGG | 0.579493 | 3.3:+94056618 | MS.gene69327:CDS |
| TGTGCGAGAACCAGGACCAT+AGG | 0.585453 | 3.3:-94056170 | None:intergenic |
| AACCAGGACCATAGGTCTTG+GGG | 0.607026 | 3.3:-94056162 | None:intergenic |
| CATGAGAATTGCCACACACA+CGG | 0.620870 | 3.3:-94056617 | None:intergenic |
| GGGGTGAGAGTTCCAAACGT+TGG | 0.647246 | 3.3:-94056143 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CCTTAATTTGAAATTAAAAT+CGG | + | chr3.3:94056423-94056442 | MS.gene69327:intron | 15.0% |
| !!! | TTTCATTTTTAAAATTTCTG+AGG | + | chr3.3:94056317-94056336 | MS.gene69327:intron | 15.0% |
| !!! | CCGATTTTAATTTCAAATTA+AGG | - | chr3.3:94056426-94056445 | None:intergenic | 20.0% |
| ! | AAACACAGTAATCAGAAATA+GGG | - | chr3.3:94056264-94056283 | None:intergenic | 25.0% |
| ! | CTTAATGAAACCAATTTCTT+TGG | - | chr3.3:94056697-94056716 | None:intergenic | 25.0% |
| ! | TAAACACAGTAATCAGAAAT+AGG | - | chr3.3:94056265-94056284 | None:intergenic | 25.0% |
| !! | CAAAGAAATTGGTTTCATTA+AGG | + | chr3.3:94056695-94056714 | MS.gene69327:CDS | 25.0% |
| AGAAATCTAGACCAACAAAT+AGG | - | chr3.3:94056741-94056760 | None:intergenic | 30.0% | |
| ATACTGCAACACAGAAAAAA+GGG | - | chr3.3:94056805-94056824 | None:intergenic | 30.0% | |
| ATATGACCTAGTGTTTAGAT+GGG | + | chr3.3:94056288-94056307 | MS.gene69327:intron | 30.0% | |
| ATCGAAGATTCATTCTTCAA+GGG | - | chr3.3:94056241-94056260 | None:intergenic | 30.0% | |
| GATTACTGTGATTCATGATT+TGG | + | chr3.3:94056349-94056368 | MS.gene69327:intron | 30.0% | |
| TCAAGGGATGAAAGAAAAAA+AGG | - | chr3.3:94056225-94056244 | None:intergenic | 30.0% | |
| TCATGAATCACAGTAATCTA+TGG | - | chr3.3:94056347-94056366 | None:intergenic | 30.0% | |
| TGATGTACTTTCCTATTTGT+TGG | + | chr3.3:94056727-94056746 | MS.gene69327:intron | 30.0% | |
| CATATGACCTAGTGTTTAGA+TGG | + | chr3.3:94056287-94056306 | MS.gene69327:intron | 35.0% | |
| GATACTGCAACACAGAAAAA+AGG | - | chr3.3:94056806-94056825 | None:intergenic | 35.0% | |
| GATCGAAGATTCATTCTTCA+AGG | - | chr3.3:94056242-94056261 | None:intergenic | 35.0% | |
| CAATTTCTTTGGCATTGCTG+TGG | - | chr3.3:94056686-94056705 | None:intergenic | 40.0% | |
| CACAGCAATGCCAAAGAAAT+TGG | + | chr3.3:94056684-94056703 | MS.gene69327:CDS | 40.0% | |
| TCGAATCCCATCTAAACACT+AGG | - | chr3.3:94056297-94056316 | None:intergenic | 40.0% | |
| TTAAAACCGTCTCCAATGCT+AGG | + | chr3.3:94056392-94056411 | MS.gene69327:intron | 40.0% | |
| ! | CATGGATTGATCAGGAAGTA+CGG | + | chr3.3:94056636-94056655 | MS.gene69327:CDS | 40.0% |
| ! | GCAATTCTCATGGATTGATC+AGG | + | chr3.3:94056628-94056647 | MS.gene69327:CDS | 40.0% |
| ACACACACGGCTACAAAACA+CGG | - | chr3.3:94056607-94056626 | None:intergenic | 45.0% | |
| CATGAGAATTGCCACACACA+CGG | - | chr3.3:94056620-94056639 | None:intergenic | 45.0% | |
| ! | AAGGCATCTTGACCTAGCAT+TGG | - | chr3.3:94056407-94056426 | None:intergenic | 45.0% |
| ! | TCTTGACCTAGCATTGGAGA+CGG | - | chr3.3:94056401-94056420 | None:intergenic | 45.0% |
| AACCAGGACCATAGGTCTTG+GGG | - | chr3.3:94056165-94056184 | None:intergenic | 50.0% | |
| AACTCTCACCCCAAGACCTA+TGG | + | chr3.3:94056154-94056173 | MS.gene69327:CDS | 50.0% | |
| AGAACCAGGACCATAGGTCT+TGG | - | chr3.3:94056167-94056186 | None:intergenic | 50.0% | |
| CGTGTGTGTGGCAATTCTCA+TGG | + | chr3.3:94056618-94056637 | MS.gene69327:CDS | 50.0% | |
| GAACCAGGACCATAGGTCTT+GGG | - | chr3.3:94056166-94056185 | None:intergenic | 50.0% | |
| TCATACCATGTGCGAGAACC+AGG | - | chr3.3:94056181-94056200 | None:intergenic | 50.0% | |
| ! | GTGTTTTGTAGCCGTGTGTG+TGG | + | chr3.3:94056606-94056625 | MS.gene69327:intron | 50.0% |
| AGTACGGACTCATGTGCTGC+AGG | + | chr3.3:94056652-94056671 | MS.gene69327:CDS | 55.0% | |
| GGGGTGAGAGTTCCAAACGT+TGG | - | chr3.3:94056146-94056165 | None:intergenic | 55.0% | |
| TGTGCGAGAACCAGGACCAT+AGG | - | chr3.3:94056173-94056192 | None:intergenic | 55.0% | |
| ! | ATGGTCCTGGTTCTCGCACA+TGG | + | chr3.3:94056173-94056192 | MS.gene69327:CDS | 55.0% |
| CACCCCAAGACCTATGGTCC+TGG | + | chr3.3:94056160-94056179 | MS.gene69327:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 94056133 | 94056830 | 94056133 | ID=MS.gene69327 |
| chr3.3 | mRNA | 94056133 | 94056830 | 94056133 | ID=MS.gene69327.t1;Parent=MS.gene69327 |
| chr3.3 | exon | 94056133 | 94056194 | 94056133 | ID=MS.gene69327.t1.exon1;Parent=MS.gene69327.t1 |
| chr3.3 | CDS | 94056133 | 94056194 | 94056133 | ID=cds.MS.gene69327.t1;Parent=MS.gene69327.t1 |
| chr3.3 | exon | 94056617 | 94056716 | 94056617 | ID=MS.gene69327.t1.exon2;Parent=MS.gene69327.t1 |
| chr3.3 | CDS | 94056617 | 94056716 | 94056617 | ID=cds.MS.gene69327.t1;Parent=MS.gene69327.t1 |
| chr3.3 | exon | 94056822 | 94056830 | 94056822 | ID=MS.gene69327.t1.exon3;Parent=MS.gene69327.t1 |
| chr3.3 | CDS | 94056822 | 94056830 | 94056822 | ID=cds.MS.gene69327.t1;Parent=MS.gene69327.t1 |
| Gene Sequence |
| Protein sequence |
