Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52771.t1 | XP_003610623.1 | 100 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 1.40E-27 | 131.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52771.t1 | Q680P8 | 92.9 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 4.4e-27 | 120.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52771.t1 | G7K0X3 | 100.0 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.8e-28 | 131.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057237 | MS.gene52771 | 0.832763 | 7.85E-56 | -1.69E-46 |
| MS.gene060279 | MS.gene52771 | 0.831068 | 2.06E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene52771 | MS.gene92361 | PPI |
| MS.gene060279 | MS.gene52771 | PPI |
| MS.gene40529 | MS.gene52771 | PPI |
| MS.gene024965 | MS.gene52771 | PPI |
| MS.gene014345 | MS.gene52771 | PPI |
| MS.gene71628 | MS.gene52771 | PPI |
| MS.gene046945 | MS.gene52771 | PPI |
| MS.gene064836 | MS.gene52771 | PPI |
| MS.gene52771 | MS.gene69327 | PPI |
| MS.gene52771 | MS.gene91297 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52771.t1 | MTR_5g005130 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| MS.gene52771.t1 | MTR_3g115330 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| MS.gene52771.t1 | MTR_1g106755 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 9.62e-38 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52771.t1 | AT4G33865 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene52771.t1 | AT3G44010 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene52771.t1 | AT3G43980 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
| MS.gene52771.t1 | AT3G44010 | 92.857 | 56 | 4 | 0 | 1 | 56 | 1 | 56 | 6.55e-34 | 109 |
Find 20 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGCAATGCCAAAGAAAT+TGG | 0.191911 | 3.1:+91238067 | MS.gene52771:CDS |
| CAAAGAAATTGGTTTCATTA+AGG | 0.240769 | 3.1:+91238078 | MS.gene52771:CDS |
| CTTAATGAAACCAATTTCTT+TGG | 0.274134 | 3.1:-91238077 | None:intergenic |
| CCATGGGTCACTCCAACGTT+TGG | 0.417313 | 3.1:+91237514 | None:intergenic |
| GAACCAGGACCATAGGTCTT+GGG | 0.430692 | 3.1:-91237546 | None:intergenic |
| AGAACCAGGACCATAGGTCT+TGG | 0.460388 | 3.1:-91237547 | None:intergenic |
| CACCCCAAGACCTATGGTCC+TGG | 0.491604 | 3.1:+91237543 | MS.gene52771:CDS |
| GCAATTCTCATGGATTGATC+AGG | 0.493067 | 3.1:+91238011 | MS.gene52771:CDS |
| AACTCTCACCCCAAGACCTA+TGG | 0.505813 | 3.1:+91237537 | MS.gene52771:CDS |
| TCATACCATGTGCGAGAACC+AGG | 0.516036 | 3.1:-91237561 | None:intergenic |
| AGTACGGACTCATGTGCTGC+AGG | 0.547263 | 3.1:+91238035 | MS.gene52771:CDS |
| CAATTTCTTTGGCATTGCTG+TGG | 0.550649 | 3.1:-91238066 | None:intergenic |
| ATGGTCCTGGTTCTCGCACA+TGG | 0.572066 | 3.1:+91237556 | MS.gene52771:CDS |
| CCAAACGTTGGAGTGACCCA+TGG | 0.572985 | 3.1:-91237514 | None:intergenic |
| CATGGATTGATCAGGAAGTA+CGG | 0.575110 | 3.1:+91238019 | MS.gene52771:CDS |
| CGTGTGTGTGGCAATTCTCA+TGG | 0.579493 | 3.1:+91238001 | MS.gene52771:CDS |
| TGTGCGAGAACCAGGACCAT+AGG | 0.585453 | 3.1:-91237553 | None:intergenic |
| AACCAGGACCATAGGTCTTG+GGG | 0.607026 | 3.1:-91237545 | None:intergenic |
| CATGAGAATTGCCACACACA+CGG | 0.620870 | 3.1:-91238000 | None:intergenic |
| GGGGTGAGAGTTCCAAACGT+TGG | 0.647246 | 3.1:-91237526 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CCTTAATTTGAAATTAAAAT+CGG | + | chr3.1:91237806-91237825 | MS.gene52771:intron | 15.0% |
| !!! | TTTCATTTTTAAAATTTCTG+AGG | + | chr3.1:91237700-91237719 | MS.gene52771:intron | 15.0% |
| !!! | CCGATTTTAATTTCAAATTA+AGG | - | chr3.1:91237809-91237828 | None:intergenic | 20.0% |
| ! | AAACACAGTAATCAGAAATA+GGG | - | chr3.1:91237647-91237666 | None:intergenic | 25.0% |
| ! | CTTAATGAAACCAATTTCTT+TGG | - | chr3.1:91238080-91238099 | None:intergenic | 25.0% |
| ! | TAAACACAGTAATCAGAAAT+AGG | - | chr3.1:91237648-91237667 | None:intergenic | 25.0% |
| !! | CAAAGAAATTGGTTTCATTA+AGG | + | chr3.1:91238078-91238097 | MS.gene52771:CDS | 25.0% |
| AGAAATCTAGACCAACAAAT+AGG | - | chr3.1:91238124-91238143 | None:intergenic | 30.0% | |
| ATACTGCAACACAGAAAAAA+GGG | - | chr3.1:91238187-91238206 | None:intergenic | 30.0% | |
| ATATGACCTAGTGTTTAGAT+GGG | + | chr3.1:91237671-91237690 | MS.gene52771:intron | 30.0% | |
| ATCGAAGATTCATTCTTCAA+GGG | - | chr3.1:91237624-91237643 | None:intergenic | 30.0% | |
| GATTACTGTGATTCATGATT+TGG | + | chr3.1:91237732-91237751 | MS.gene52771:intron | 30.0% | |
| TCAAGGGATGAAAGAAAAAA+AGG | - | chr3.1:91237608-91237627 | None:intergenic | 30.0% | |
| TCATGAATCACAGTAATCTA+TGG | - | chr3.1:91237730-91237749 | None:intergenic | 30.0% | |
| TGATGTACTTTCCTATTTGT+TGG | + | chr3.1:91238110-91238129 | MS.gene52771:intron | 30.0% | |
| CATATGACCTAGTGTTTAGA+TGG | + | chr3.1:91237670-91237689 | MS.gene52771:intron | 35.0% | |
| GATACTGCAACACAGAAAAA+AGG | - | chr3.1:91238188-91238207 | None:intergenic | 35.0% | |
| GATCGAAGATTCATTCTTCA+AGG | - | chr3.1:91237625-91237644 | None:intergenic | 35.0% | |
| CAATTTCTTTGGCATTGCTG+TGG | - | chr3.1:91238069-91238088 | None:intergenic | 40.0% | |
| CACAGCAATGCCAAAGAAAT+TGG | + | chr3.1:91238067-91238086 | MS.gene52771:CDS | 40.0% | |
| TCGAATCCCATCTAAACACT+AGG | - | chr3.1:91237680-91237699 | None:intergenic | 40.0% | |
| ! | CATGGATTGATCAGGAAGTA+CGG | + | chr3.1:91238019-91238038 | MS.gene52771:CDS | 40.0% |
| ! | GCAATTCTCATGGATTGATC+AGG | + | chr3.1:91238011-91238030 | MS.gene52771:CDS | 40.0% |
| ACACACACGGCTACAAAACA+CGG | - | chr3.1:91237990-91238009 | None:intergenic | 45.0% | |
| CATGAGAATTGCCACACACA+CGG | - | chr3.1:91238003-91238022 | None:intergenic | 45.0% | |
| TTAAAGCCGTCTCCAATGCT+AGG | + | chr3.1:91237775-91237794 | MS.gene52771:intron | 45.0% | |
| ! | AAGGCATCTTGACCTAGCAT+TGG | - | chr3.1:91237790-91237809 | None:intergenic | 45.0% |
| ! | TCTTGACCTAGCATTGGAGA+CGG | - | chr3.1:91237784-91237803 | None:intergenic | 45.0% |
| AACCAGGACCATAGGTCTTG+GGG | - | chr3.1:91237548-91237567 | None:intergenic | 50.0% | |
| AACTCTCACCCCAAGACCTA+TGG | + | chr3.1:91237537-91237556 | MS.gene52771:CDS | 50.0% | |
| AGAACCAGGACCATAGGTCT+TGG | - | chr3.1:91237550-91237569 | None:intergenic | 50.0% | |
| CGTGTGTGTGGCAATTCTCA+TGG | + | chr3.1:91238001-91238020 | MS.gene52771:CDS | 50.0% | |
| GAACCAGGACCATAGGTCTT+GGG | - | chr3.1:91237549-91237568 | None:intergenic | 50.0% | |
| TCATACCATGTGCGAGAACC+AGG | - | chr3.1:91237564-91237583 | None:intergenic | 50.0% | |
| ! | GTGTTTTGTAGCCGTGTGTG+TGG | + | chr3.1:91237989-91238008 | MS.gene52771:intron | 50.0% |
| AGTACGGACTCATGTGCTGC+AGG | + | chr3.1:91238035-91238054 | MS.gene52771:CDS | 55.0% | |
| GGGGTGAGAGTTCCAAACGT+TGG | - | chr3.1:91237529-91237548 | None:intergenic | 55.0% | |
| TGTGCGAGAACCAGGACCAT+AGG | - | chr3.1:91237556-91237575 | None:intergenic | 55.0% | |
| ! | ATGGTCCTGGTTCTCGCACA+TGG | + | chr3.1:91237556-91237575 | MS.gene52771:CDS | 55.0% |
| CACCCCAAGACCTATGGTCC+TGG | + | chr3.1:91237543-91237562 | MS.gene52771:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 91237516 | 91238212 | 91237516 | ID=MS.gene52771 |
| chr3.1 | mRNA | 91237516 | 91238212 | 91237516 | ID=MS.gene52771.t1;Parent=MS.gene52771 |
| chr3.1 | exon | 91237516 | 91237577 | 91237516 | ID=MS.gene52771.t1.exon1;Parent=MS.gene52771.t1 |
| chr3.1 | CDS | 91237516 | 91237577 | 91237516 | ID=cds.MS.gene52771.t1;Parent=MS.gene52771.t1 |
| chr3.1 | exon | 91238000 | 91238099 | 91238000 | ID=MS.gene52771.t1.exon2;Parent=MS.gene52771.t1 |
| chr3.1 | CDS | 91238000 | 91238099 | 91238000 | ID=cds.MS.gene52771.t1;Parent=MS.gene52771.t1 |
| chr3.1 | exon | 91238204 | 91238212 | 91238204 | ID=MS.gene52771.t1.exon3;Parent=MS.gene52771.t1 |
| chr3.1 | CDS | 91238204 | 91238212 | 91238204 | ID=cds.MS.gene52771.t1;Parent=MS.gene52771.t1 |
| Gene Sequence |
| Protein sequence |
