AlfalfaGEDB | MS.gene71856

M. sativa cultivar XinJiangDaYe / MS.gene71856

KEGG: K00487
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0020037 GO:0043167 GO:0043169 GO:0046872 GO:0046906 GO:0046914 GO:0048037 GO:0055114 GO:0097159 GO:1901363
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71856.t1	XP_013470209.1	97.8	448	10	0	1	448	89	536	1.50E-254	888.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71856.t1	H2DH22	82.3	451	74	3	1	448	84	531	2.3e-214	746.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71856.t1	G7ZZA6	97.8	448	10	0	1	448	89	536	1.1e-254	888.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052606	MS.gene71856	0.81488	1.23E-51	-1.69E-46
MS.gene053108	MS.gene71856	0.808807	2.58E-50	-1.69E-46
MS.gene053421	MS.gene71856	0.832717	8.06E-56	-1.69E-46
MS.gene058171	MS.gene71856	0.803298	3.73E-49	-1.69E-46
MS.gene058175	MS.gene71856	0.803919	2.77E-49	-1.69E-46
MS.gene059526	MS.gene71856	0.81473	1.33E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71856.t1	MTR_1g111240	97.768	448	10	0	1	448	89	536	0.0	914
MS.gene71856.t1	MTR_5g075450	65.556	450	147	4	2	447	58	503	0.0	618
MS.gene71856.t1	MTR_2g065360	31.304	460	277	12	2	444	31	468	2.56e-69	228
MS.gene71856.t1	MTR_3g058210	29.677	465	291	12	2	449	62	507	7.60e-68	225
MS.gene71856.t1	MTR_3g057800	30.872	447	278	10	2	437	60	486	6.49e-67	222
MS.gene71856.t1	MTR_3g058220	30.131	458	288	11	2	447	61	498	1.03e-66	222
MS.gene71856.t1	MTR_5g094550	31.579	437	270	11	5	431	67	484	1.54e-65	219
MS.gene71856.t1	MTR_3g025260	31.707	451	300	7	2	447	56	503	1.73e-65	219
MS.gene71856.t1	MTR_5g094570	29.806	463	285	11	1	449	63	499	1.10e-64	216
MS.gene71856.t1	MTR_5g021390	30.952	462	284	11	2	447	68	510	1.11e-64	217

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71856.t1	AT2G30490	65.410	451	149	4	2	449	58	504	0.0	617
MS.gene71856.t1	AT4G22710	33.105	438	267	11	4	431	80	501	1.13e-72	238
MS.gene71856.t1	AT4G22690	32.877	438	268	11	4	431	111	532	5.30e-72	237
MS.gene71856.t1	AT2G40890	32.258	434	277	6	6	431	55	479	1.22e-70	232
MS.gene71856.t1	AT1G74550	31.871	433	265	8	6	431	55	464	4.90e-70	230
MS.gene71856.t1	AT5G07990	30.263	456	295	12	2	447	57	499	7.45e-65	217
MS.gene71856.t1	AT3G26280	31.603	443	275	9	2	430	56	484	1.78e-63	213
MS.gene71856.t1	AT3G26280	31.603	443	275	9	2	430	73	501	2.10e-63	213
MS.gene71856.t1	AT4G36220	30.043	466	284	12	2	445	64	509	1.13e-62	211
MS.gene71856.t1	AT4G12300	31.663	439	279	10	2	431	66	492	1.15e-62	211

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Find 88 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACTATGGTCCATGGAAT+GGG	0.462904	1.3:+75845206	MS.gene71856:CDS
AACCTTGAAGAGGCTAAGCT+AGG	0.612366	1.3:+75845408	MS.gene71856:CDS
AAGATTGATGTTAGTGAAAA+AGG	0.236921	1.3:+75845714	MS.gene71856:CDS
AATGAAGAACTTGTGTGGCA+AGG	0.651530	1.3:-75844075	None:intergenic
AATGACATTGTTAAAAGTGA+AGG	0.546134	1.3:+75844310	MS.gene71856:CDS
AATGGAATTGATGCTGTTGC+TGG	0.494586	1.3:+75845549	MS.gene71856:CDS
AATTGGATGGAACAAAACAG+TGG	0.704408	1.3:-75845769	None:intergenic
ACAACTACCTTAGACTCTTT+TGG	0.248706	1.3:-75845444	None:intergenic
ACAACTACTACATTGAGAAG+AGG	0.634874	1.3:+75844581	MS.gene71856:CDS
ACACAAGTTCTTCATTCACA+AGG	0.648627	1.3:+75844082	MS.gene71856:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGGTTTATGGAAGAAGAAAA+TGG	+	chr1.3:75845531-75845550	MS.gene71856:CDS	30.0%
ATACTTGCATTGCCAATATT+GGG	+	chr1.3:75845636-75845655	MS.gene71856:CDS	30.0%
ATTGATGTTAGTGAAAAAGG+TGG	+	chr1.3:75845717-75845736	MS.gene71856:CDS	30.0%
CCAAAATTATAGTAGCATGT+GGG	+	chr1.3:75844249-75844268	MS.gene71856:CDS	30.0%
TATACTTGCATTGCCAATAT+TGG	+	chr1.3:75845635-75845654	MS.gene71856:CDS	30.0%
TATTAGTCAAGTTTCTTGTC+TGG	+	chr1.3:75844626-75844645	MS.gene71856:intron	30.0%
TCCAAAATTATAGTAGCATG+TGG	+	chr1.3:75844248-75844267	MS.gene71856:CDS	30.0%
TGCAAATAATCCATTATGGT+GGG	+	chr1.3:75845482-75845501	MS.gene71856:CDS	30.0%
TGCTTAACTGCTTATGATAT+AGG	+	chr1.3:75844682-75844701	MS.gene71856:intron	30.0%
TTGCAAATAATCCATTATGG+TGG	+	chr1.3:75845481-75845500	MS.gene71856:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	75843989	75845797	75843989	ID=MS.gene71856
chr1.3	mRNA	75843989	75845797	75843989	ID=MS.gene71856.t1;Parent=MS.gene71856
chr1.3	exon	75843989	75844605	75843989	ID=MS.gene71856.t1.exon1;Parent=MS.gene71856.t1
chr1.3	CDS	75843989	75844605	75843989	ID=cds.MS.gene71856.t1;Parent=MS.gene71856.t1
chr1.3	exon	75845065	75845797	75845065	ID=MS.gene71856.t1.exon2;Parent=MS.gene71856.t1
chr1.3	CDS	75845065	75845797	75845065	ID=cds.MS.gene71856.t1;Parent=MS.gene71856.t1

Gene Sequence

>MS.gene71856

ATGCTTGCATCCATGTCACAAACTTATGGACCACTTTTCCTACTCAAACTTGGTTCCAAAAACTTGGTTGTTGTCTCTGATCCAAACCTTGCCACACAAGTTCTTCATTCACAAGGTGTCGAATTCGGTTCTCGTCCTCGCAACGTTGTCTTTGATATCTTCACTGGTAATGGACAAGACATGGTTTTCACTGTCTATGGTGATCACTGGCGCAAAATGCGAAGAATCATGACATTGCCGTTTTTCACTAACAAAGTTGTCCAAAATTATAGTAGCATGTGGGAACAAGAGATGGATTTGGTAGTTCAAGACCTAAAGGCTAATGACATTGTTAAAAGTGAAGGCATTGTAATTAGAAAACGTCTTCAGCTGATGTTGTATAATATCATGTATAGGATGATGTTTGATGCCAAATTTGAATCTCAAGAAGATCCTTTGTTTATTCAGGCTACAAGGTTTAACTCTGAGAGAAGCCGTTTGGCACAGAGTTTTGAATATAATTATGGTGATTTTATTCCCTTGCTTAGGCCATTTTTGAGAGGCTACCTTAACAAGTGCAAAGACTTGCAAACTAGGAGGCTTGCATTTTTCAACAACTACTACATTGAGAAGAGGAGGTAAGTAATTGATCTATTAATATTAGTCAAGTTTCTTGTCTGGATTTACAGCTTAATTAAGTGCTTATTGCATAGGTGCTTAACTGCTTATGATATAGGTGCTTAAATATAAGTTTTTTTTTTTCTCATAGAGAGAATTAGAAATAAGTTGATAATAGCTAACGGACATGTCATAATTTGTTACCATAAACTATTCCAAATAGTCTCAAATACTTATGCCAGTAGATAAACTCAAACGTGTTCTCTGAAGCGGTCTCACAAGTGTGGTTAACCACGAAATTGAGGATCACGTAAACATAATGCAACACCTTTACACATAGATCCAATCACAATTCACCCTATTGGCATTTTGTTATACTACCAAAAATACATCCACAAAAATCCATATTAATTATATAATTTTTTTCAACTGATTTTCCATTTATATGTAACATAGAGTATTTTTTTTTTCTTCTCCAGAAAAATAATGGCAGCCAATGGGGAGAAGCATGAGATAAACTGTGCAATTGATCATATCATAGATGCTGAGATGAAAGGTGAAATAAGTGAAGATAATGTGACTTACATAGTAGAGAACATTAATGTTGCTGCAATAGAGACAACACTATGGTCCATGGAATGGGCAATAGCAGAGTTAGTGAATCATCCAGAAGTTCAAACCAAAATTCGTGATGAGATATCAAAAGTGCTTAAAGGTGAACAAGTCACAGAATCAAACTTAAACCAATTACCATATTTACAAGCTACATTGAAAGAGATATTGAGATTGCATACACCAATTCCATTGTTAGTGCCACACATGAACCTTGAAGAGGCTAAGCTAGGAGAGTACACTATTCCAAAAGAGTCTAAGGTAGTTGTTAATGCTTGGTGGCTTGCAAATAATCCATTATGGTGGGAAAAGCCAGAGGAGTTCAGGCCAGAGAGGTTTATGGAAGAAGAAAATGGAATTGATGCTGTTGCTGGTGGAGGGAAAGTTGATTTTAGGTTTGTGCCATTTGGTGTTGGAAGAAGGAGTTGTCCTGGAATTATACTTGCATTGCCAATATTGGGATTGGTTATTGGAAAGTTGGTGTCAAATTTTGAGATGAAGGGTCCAAATGGGACAAAGATTGATGTTAGTGAAAAAGGTGGACAATTTAGCTTGCACATTGCCAACCACTCCACTGTTTTGTTCCATCCAATTAATTAG

Protein sequence

>MS.gene71856.t1

MLASMSQTYGPLFLLKLGSKNLVVVSDPNLATQVLHSQGVEFGSRPRNVVFDIFTGNGQDMVFTVYGDHWRKMRRIMTLPFFTNKVVQNYSSMWEQEMDLVVQDLKANDIVKSEGIVIRKRLQLMLYNIMYRMMFDAKFESQEDPLFIQATRFNSERSRLAQSFEYNYGDFIPLLRPFLRGYLNKCKDLQTRRLAFFNNYYIEKRRKIMAANGEKHEINCAIDHIIDAEMKGEISEDNVTYIVENINVAAIETTLWSMEWAIAELVNHPEVQTKIRDEISKVLKGEQVTESNLNQLPYLQATLKEILRLHTPIPLLVPHMNLEEAKLGEYTIPKESKVVVNAWWLANNPLWWEKPEEFRPERFMEEENGIDAVAGGGKVDFRFVPFGVGRRSCPGIILALPILGLVIGKLVSNFEMKGPNGTKIDVSEKGGQFSLHIANHSTVLFHPIN