Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71957.t1 | KEH31643.1 | 96.9 | 98 | 3 | 0 | 1 | 98 | 1 | 98 | 9.30E-40 | 172.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71957.t1 | A0A072URE5 | 96.9 | 98 | 3 | 0 | 1 | 98 | 1 | 98 | 6.7e-40 | 172.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene71957 | 0.802637 | 5.10E-49 | -1.69E-46 |
| MS.gene052666 | MS.gene71957 | 0.814168 | 1.77E-51 | -1.69E-46 |
| MS.gene060483 | MS.gene71957 | 0.843928 | 1.04E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71957.t1 | MTR_4g100820 | 96.939 | 98 | 3 | 0 | 1 | 98 | 1 | 98 | 4.75e-61 | 181 |
| MS.gene71957.t1 | MTR_4g100820 | 96.667 | 90 | 3 | 0 | 1 | 90 | 1 | 90 | 7.97e-55 | 165 |
| MS.gene71957.t1 | MTR_4g100820 | 94.505 | 91 | 5 | 0 | 1 | 91 | 1 | 91 | 3.08e-54 | 164 |
| MS.gene71957.t1 | MTR_4g100820 | 96.591 | 88 | 3 | 0 | 1 | 88 | 1 | 88 | 6.13e-53 | 160 |
| MS.gene71957.t1 | MTR_3g109720 | 46.250 | 80 | 43 | 0 | 19 | 98 | 14 | 93 | 6.53e-15 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene71957.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene71957.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene71957.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene71957.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene71957.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene71957.t1 | AT5G47455 | 68.333 | 60 | 19 | 0 | 39 | 98 | 49 | 108 | 5.53e-19 | 75.5 |
| MS.gene71957.t1 | AT5G47455 | 68.333 | 60 | 19 | 0 | 39 | 98 | 49 | 108 | 5.53e-19 | 75.5 |
| MS.gene71957.t1 | AT5G47455 | 68.333 | 60 | 19 | 0 | 39 | 98 | 49 | 108 | 5.53e-19 | 75.5 |
| MS.gene71957.t1 | AT4G17310 | 51.685 | 89 | 42 | 1 | 1 | 88 | 1 | 89 | 2.43e-17 | 70.9 |
| MS.gene71957.t1 | AT4G17310 | 51.685 | 89 | 42 | 1 | 1 | 88 | 1 | 89 | 4.22e-17 | 70.1 |
| MS.gene71957.t1 | AT5G47455 | 65.385 | 52 | 18 | 0 | 39 | 90 | 49 | 100 | 2.70e-13 | 60.5 |
| MS.gene71957.t1 | AT5G47455 | 65.385 | 52 | 18 | 0 | 39 | 90 | 49 | 100 | 2.70e-13 | 60.5 |
| MS.gene71957.t1 | AT5G47455 | 65.385 | 52 | 18 | 0 | 39 | 90 | 49 | 100 | 2.70e-13 | 60.5 |
| MS.gene71957.t1 | AT5G47455 | 65.385 | 52 | 18 | 0 | 39 | 90 | 49 | 100 | 2.70e-13 | 60.5 |
| MS.gene71957.t1 | AT5G11630 | 41.414 | 99 | 51 | 4 | 1 | 98 | 1 | 93 | 4.99e-13 | 59.7 |
| MS.gene71957.t1 | AT5G11630 | 41.414 | 99 | 51 | 4 | 1 | 98 | 1 | 93 | 4.99e-13 | 59.7 |
| MS.gene71957.t1 | AT5G47455 | 62.963 | 54 | 19 | 1 | 39 | 91 | 49 | 102 | 8.11e-12 | 57.4 |
| MS.gene71957.t1 | AT5G47455 | 60.377 | 53 | 21 | 0 | 39 | 91 | 49 | 101 | 9.56e-12 | 56.6 |
| MS.gene71957.t1 | AT5G47455 | 60.377 | 53 | 21 | 0 | 39 | 91 | 49 | 101 | 9.56e-12 | 56.6 |
| MS.gene71957.t1 | AT5G47455 | 64.000 | 50 | 18 | 0 | 39 | 88 | 49 | 98 | 1.66e-11 | 56.2 |
Find 28 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCAGTGGTGGTTGGTTT+GGG | 0.163477 | 8.3:+20401183 | None:intergenic |
| TCCAAGCTCCGGCGCAATTC+TGG | 0.241996 | 8.3:+20401131 | None:intergenic |
| TGTGGCAGTGGTGGTTGGTT+TGG | 0.246581 | 8.3:+20401182 | None:intergenic |
| GGTTGGTTTGGGGATGGATG+AGG | 0.393701 | 8.3:+20401194 | None:intergenic |
| GGATTTAATTGCGGATTTGA+TGG | 0.409263 | 8.3:+20401227 | None:intergenic |
| AGTGGTGGTTGGTTTGGGGA+TGG | 0.417037 | 8.3:+20401188 | None:intergenic |
| TCCAGAATTGCGCCGGAGCT+TGG | 0.422852 | 8.3:-20401132 | MS.gene71957:CDS |
| CACGTCTCAGCGTAACTTCG+AGG | 0.434582 | 8.3:-20401053 | MS.gene71957:CDS |
| GATGGATGAGGTGCGAATGT+TGG | 0.451724 | 8.3:+20401206 | None:intergenic |
| GACTGCACGCATCCAAGCTC+CGG | 0.453744 | 8.3:+20401120 | None:intergenic |
| CTGCTGCATGCGCAAGCCAT+TGG | 0.469294 | 8.3:+20401276 | None:intergenic |
| TGGCAGTGGTGGTTGGTTTG+GGG | 0.472598 | 8.3:+20401184 | None:intergenic |
| GCCGCAACTGCGCTGTGAAG+TGG | 0.483273 | 8.3:+20401090 | None:intergenic |
| ACTCTCTGCTGCACCTCTCC+CGG | 0.490651 | 8.3:-20401006 | MS.gene71957:CDS |
| GAAGATGTGGCAGTGGTGGT+TGG | 0.504244 | 8.3:+20401177 | None:intergenic |
| GAACTGTCAATCGCTTTCAC+AGG | 0.505088 | 8.3:-20401031 | MS.gene71957:CDS |
| GCCACTTCACAGCGCAGTTG+CGG | 0.509854 | 8.3:-20401091 | MS.gene71957:CDS |
| AAAAGAAAACATTAGAGTCC+GGG | 0.554992 | 8.3:+20400988 | None:intergenic |
| ATTTGCATCCAGAATTGCGC+CGG | 0.566247 | 8.3:-20401139 | MS.gene71957:CDS |
| GCGAATGTTGGATTTAATTG+CGG | 0.569994 | 8.3:+20401218 | None:intergenic |
| TGGAGAAGATGTGGCAGTGG+TGG | 0.576473 | 8.3:+20401173 | None:intergenic |
| CAATTCTGGATGCAAATGAT+CGG | 0.592552 | 8.3:+20401145 | None:intergenic |
| AAAACATTAGAGTCCGGGAG+AGG | 0.598769 | 8.3:+20400993 | None:intergenic |
| GATGCAAATGATCGGCGAAG+TGG | 0.621136 | 8.3:+20401153 | None:intergenic |
| AACTGTCAATCGCTTTCACA+GGG | 0.623914 | 8.3:-20401030 | MS.gene71957:CDS |
| ACTTCACAGCGCAGTTGCGG+CGG | 0.632388 | 8.3:-20401088 | MS.gene71957:CDS |
| TCGGCGAAGTGGAGAAGATG+TGG | 0.637549 | 8.3:+20401164 | None:intergenic |
| AAGTGGAGAAGATGTGGCAG+TGG | 0.639588 | 8.3:+20401170 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAATTCTGGATGCAAATGAT+CGG | + | chr8.3:20401130-20401149 | None:intergenic | 35.0% | |
| GCGAATGTTGGATTTAATTG+CGG | + | chr8.3:20401057-20401076 | None:intergenic | 35.0% | |
| GGATTTAATTGCGGATTTGA+TGG | + | chr8.3:20401048-20401067 | None:intergenic | 35.0% | |
| AACTGTCAATCGCTTTCACA+GGG | - | chr8.3:20401242-20401261 | MS.gene71957:CDS | 40.0% | |
| ATTTGCATCCAGAATTGCGC+CGG | - | chr8.3:20401133-20401152 | MS.gene71957:CDS | 45.0% | |
| GAACTGTCAATCGCTTTCAC+AGG | - | chr8.3:20401241-20401260 | MS.gene71957:CDS | 45.0% | |
| AAGTGGAGAAGATGTGGCAG+TGG | + | chr8.3:20401105-20401124 | None:intergenic | 50.0% | |
| GATGCAAATGATCGGCGAAG+TGG | + | chr8.3:20401122-20401141 | None:intergenic | 50.0% | |
| GATGGATGAGGTGCGAATGT+TGG | + | chr8.3:20401069-20401088 | None:intergenic | 50.0% | |
| CACGTCTCAGCGTAACTTCG+AGG | - | chr8.3:20401219-20401238 | MS.gene71957:CDS | 55.0% | |
| GAAGATGTGGCAGTGGTGGT+TGG | + | chr8.3:20401098-20401117 | None:intergenic | 55.0% | |
| TCGGCGAAGTGGAGAAGATG+TGG | + | chr8.3:20401111-20401130 | None:intergenic | 55.0% | |
| TGGAGAAGATGTGGCAGTGG+TGG | + | chr8.3:20401102-20401121 | None:intergenic | 55.0% | |
| ! | TGTGGCAGTGGTGGTTGGTT+TGG | + | chr8.3:20401093-20401112 | None:intergenic | 55.0% |
| !! | AGTGGTGGTTGGTTTGGGGA+TGG | + | chr8.3:20401087-20401106 | None:intergenic | 55.0% |
| !! | GGTTGGTTTGGGGATGGATG+AGG | + | chr8.3:20401081-20401100 | None:intergenic | 55.0% |
| !! | GTGGCAGTGGTGGTTGGTTT+GGG | + | chr8.3:20401092-20401111 | None:intergenic | 55.0% |
| !! | TGGCAGTGGTGGTTGGTTTG+GGG | + | chr8.3:20401091-20401110 | None:intergenic | 55.0% |
| ACTCTCTGCTGCACCTCTCC+CGG | - | chr8.3:20401266-20401285 | MS.gene71957:CDS | 60.0% | |
| ACTTCACAGCGCAGTTGCGG+CGG | - | chr8.3:20401184-20401203 | MS.gene71957:CDS | 60.0% | |
| GACTGCACGCATCCAAGCTC+CGG | + | chr8.3:20401155-20401174 | None:intergenic | 60.0% | |
| GCCACTTCACAGCGCAGTTG+CGG | - | chr8.3:20401181-20401200 | MS.gene71957:CDS | 60.0% | |
| TCCAAGCTCCGGCGCAATTC+TGG | + | chr8.3:20401144-20401163 | None:intergenic | 60.0% | |
| TCCAGAATTGCGCCGGAGCT+TGG | - | chr8.3:20401140-20401159 | MS.gene71957:CDS | 60.0% | |
| GCCGCAACTGCGCTGTGAAG+TGG | + | chr8.3:20401185-20401204 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 20400999 | 20401295 | 20400999 | ID=MS.gene71957 |
| chr8.3 | mRNA | 20400999 | 20401295 | 20400999 | ID=MS.gene71957.t1;Parent=MS.gene71957 |
| chr8.3 | exon | 20400999 | 20401295 | 20400999 | ID=MS.gene71957.t1.exon1;Parent=MS.gene71957.t1 |
| chr8.3 | CDS | 20400999 | 20401295 | 20400999 | ID=cds.MS.gene71957.t1;Parent=MS.gene71957.t1 |
| Gene Sequence |
| Protein sequence |
