AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052666


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052666.t1 MTR_8g105110 100.000 154 0 0 1 154 1 154 4.92e-111 312
MS.gene052666.t1 MTR_1g070450 95.455 154 5 1 1 154 1 152 4.61e-104 294
MS.gene052666.t1 MTR_4g075450 92.857 154 9 1 1 154 1 152 4.90e-92 264
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052666.t1 AT5G09500 87.662 154 15 1 1 154 1 150 1.07e-93 268
MS.gene052666.t1 AT5G09510 91.558 154 11 1 1 154 1 152 3.57e-92 264
MS.gene052666.t1 AT1G04270 91.558 154 11 1 1 154 1 152 4.12e-92 264
MS.gene052666.t1 AT1G04270 90.909 154 11 2 1 154 1 151 1.39e-89 258
MS.gene052666.t1 AT5G09490 81.818 154 26 1 1 154 1 152 9.71e-89 255
MS.gene052666.t1 AT5G43640 85.906 149 21 0 6 154 1 149 1.10e-82 240
MS.gene052666.t1 AT5G09510 94.915 118 6 0 37 154 1 118 2.08e-71 210
MS.gene052666.t1 AT5G63070 62.500 160 54 2 1 154 1 160 3.08e-64 194
MS.gene052666.t1 AT1G33850 83.607 61 10 0 12 72 7 67 1.12e-19 78.2

Find 43 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATTTCAACTTGGTTAA+AGG 0.236449 4.1:-1815100 None:intergenic
GAGATGATTGGGAGCATTAT+TGG 0.247005 4.1:+1815060 MS.gene052666:CDS
CAGTTTCAGAGGAGTTGATT+TGG 0.292075 4.1:+1813826 MS.gene052666:CDS
CCAGGTCTACCATGCTTAAC+AGG 0.341486 4.1:-1815171 None:intergenic
TCAGTGCTCGTGCTCGTAGA+AGG 0.367007 4.1:+1813891 MS.gene052666:CDS
TTCTTGAATGTTCTCTTCTT+CGG 0.377124 4.1:-1813800 None:intergenic
AGATGGGTCCTAACAGGTTC+GGG 0.405557 4.1:-1815015 None:intergenic
GTGAGAAGCCCGAACCTGTT+AGG 0.409377 4.1:+1815007 MS.gene052666:CDS
GATGCAGCAGCCGCCGCCGC+GGG 0.422266 4.1:+1813773 MS.gene052666:CDS
TTACGAAGATGGGTCCTAAC+AGG 0.435557 4.1:-1815021 None:intergenic
AAGATGGGTCCTAACAGGTT+CGG 0.450751 4.1:-1815016 None:intergenic
CAATGATCATGTTACGAAGA+TGG 0.465315 4.1:-1815032 None:intergenic
ATCATTGTACCTGAGATGAT+TGG 0.468347 4.1:+1815048 MS.gene052666:CDS
TTCAAGAAGTTCAGTTTCAG+AGG 0.481387 4.1:+1813815 MS.gene052666:CDS
AGGTAATGACCGATCATCTC+AGG 0.492365 4.1:-1815123 None:intergenic
CCTGTTAAGCATGGTAGACC+TGG 0.509940 4.1:+1815171 MS.gene052666:CDS
AATGATCATGTTACGAAGAT+GGG 0.516658 4.1:-1815031 None:intergenic
GCATTATTGGAGTGTACAAT+GGG 0.517140 4.1:+1815073 MS.gene052666:CDS
ACAGGTTCGGGCTTCTCACC+AGG 0.517899 4.1:-1815003 None:intergenic
CTTCGGTACTCCCGCGGCGG+CGG 0.518043 4.1:-1813783 None:intergenic
TTGGTGCTACTCACTCCTCC+AGG 0.521787 4.1:+1815196 MS.gene052666:CDS
ATAATGCTCCCAATCATCTC+AGG 0.528225 4.1:-1815057 None:intergenic
TCATTGTACCTGAGATGATT+GGG 0.539509 4.1:+1815049 MS.gene052666:CDS
GCTTACGAGTGAGACCTCTC+TGG 0.541580 4.1:-1813916 None:intergenic
TCTCAGGTTTGATTTCAACT+TGG 0.546765 4.1:-1815107 None:intergenic
AGCATTATTGGAGTGTACAA+TGG 0.558872 4.1:+1815072 MS.gene052666:CDS
TAGAAAAGGGAGGCACCACC+TGG 0.561975 4.1:+1814985 MS.gene052666:intron
TCACTTGAGAGGAATGAACC+TGG 0.566509 4.1:-1815214 None:intergenic
AGATGCAGCAGCCGCCGCCG+CGG 0.579389 4.1:+1813772 MS.gene052666:CDS
GAGTGAGTAGCACCAATACC+AGG 0.584094 4.1:-1815189 None:intergenic
GAAATCAAACCTGAGATGAT+CGG 0.585387 4.1:+1815114 MS.gene052666:CDS
AAGCATGGTAGACCTGGTAT+TGG 0.586812 4.1:+1815177 MS.gene052666:CDS
TCATACAAGCCTGTTAAGCA+TGG 0.604339 4.1:+1815162 MS.gene052666:CDS
GAGCTTAACAAGCTCATCAG+TGG 0.607114 4.1:-1813867 None:intergenic
AGGTCTCACTCGTAAGCCCA+TGG 0.623580 4.1:+1813922 MS.gene052666:CDS
AACAAGGTGATTCACTTGAG+AGG 0.631770 4.1:-1815225 None:intergenic
ATGAGATCGAGAATTCAGCT+AGG 0.682732 4.1:-1815143 None:intergenic
GGTTCGGGCTTCTCACCAGG+TGG 0.683492 4.1:-1815000 None:intergenic
TCTCTTCTTCGGTACTCCCG+CGG 0.697626 4.1:-1813789 None:intergenic
CTTCTTCGGTACTCCCGCGG+CGG 0.700206 4.1:-1813786 None:intergenic
GAATTGGTTGTAGAAAAGGG+AGG 0.733330 4.1:+1814975 MS.gene052666:intron
CTTGAGAGGAATGAACCTGG+AGG 0.737810 4.1:-1815211 None:intergenic
GCTCGTAGAAGGTTCCAGAG+AGG 0.763484 4.1:+1813902 MS.gene052666:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTACTATTTTACAAATTTA+AGG - chr4.1:1814108-1814127 None:intergenic 10.0%
!!! AAAAAAAAGTGTTCAATTTA+AGG - chr4.1:1814451-1814470 None:intergenic 15.0%
!!! AAAAAAAGTGTTCAATTTAA+GGG - chr4.1:1814450-1814469 None:intergenic 15.0%
!!! ATTGTTATAATTTTGTGAAT+TGG + chr4.1:1814959-1814978 MS.gene052666:intron 15.0%
!!! GAACACTTTTTTTTTTTTTT+TGG + chr4.1:1814457-1814476 MS.gene052666:intron 15.0%
!!! TTTGTTATTCTTTTATTTAG+TGG + chr4.1:1814595-1814614 MS.gene052666:intron 15.0%
!!! TTTGTTATTTTGTTAATTTG+AGG + chr4.1:1814355-1814374 MS.gene052666:intron 15.0%
!! AAGGAACTAAATTGATATAT+TGG - chr4.1:1814402-1814421 None:intergenic 20.0%
!! ATATGCAAAATACTAACAAT+TGG + chr4.1:1814079-1814098 MS.gene052666:intron 20.0%
!! ATTCCAAATTCAAATTCAAT+CGG - chr4.1:1813726-1813745 None:intergenic 20.0%
!! ATTTAGTACTTAAGATAGTA+AGG - chr4.1:1814929-1814948 None:intergenic 20.0%
!! TATGCAAAATACTAACAATT+GGG + chr4.1:1814080-1814099 MS.gene052666:intron 20.0%
!! TTACCTATTATGCAAATTTA+AGG - chr4.1:1814421-1814440 None:intergenic 20.0%
!! TTTAGTACTTAAGATAGTAA+GGG - chr4.1:1814928-1814947 None:intergenic 20.0%
!!! GAAGTTTTTATTGATTTTGT+TGG + chr4.1:1814531-1814550 MS.gene052666:intron 20.0%
!!! GTTAGTATTTTGCATATTAA+AGG - chr4.1:1814076-1814095 None:intergenic 20.0%
!!! TGGTGGTTTTTTAAATTTAA+AGG + chr4.1:1814859-1814878 MS.gene052666:intron 20.0%
!!! TTGTTAATTTGTGAATTTGA+GGG + chr4.1:1814888-1814907 MS.gene052666:intron 20.0%
!!! TTTGTTAATTTGTGAATTTG+AGG + chr4.1:1814887-1814906 MS.gene052666:intron 20.0%
! AAATCCATGTTAGATTCTAT+CGG - chr4.1:1813703-1813722 None:intergenic 25.0%
! AGTAATTGCATACCAAAAAT+TGG + chr4.1:1814122-1814141 MS.gene052666:intron 25.0%
! ATTAAAATGGGTACTAAAGT+CGG - chr4.1:1814187-1814206 None:intergenic 25.0%
! ATTTAACACTACCATTCTAA+AGG + chr4.1:1814722-1814741 MS.gene052666:intron 25.0%
! GAACATGAAACCATTAAAAT+GGG - chr4.1:1814199-1814218 None:intergenic 25.0%
! GTTCCTTAAATTTGCATAAT+AGG + chr4.1:1814415-1814434 MS.gene052666:intron 25.0%
! TGAACATGAAACCATTAAAA+TGG - chr4.1:1814200-1814219 None:intergenic 25.0%
! TTTCCGATTGAATTTGAATT+TGG + chr4.1:1813720-1813739 MS.gene052666:intron 25.0%
!! AAACAACAATCTTAGCATTT+TGG + chr4.1:1814033-1814052 MS.gene052666:intron 25.0%
!! AACAACAATCTTAGCATTTT+GGG + chr4.1:1814034-1814053 MS.gene052666:intron 25.0%
!! AATAGCGATATTTAGGTTAT+AGG + chr4.1:1814824-1814843 MS.gene052666:intron 25.0%
!! AGGACTAACATCAAAATTTT+TGG - chr4.1:1814633-1814652 None:intergenic 25.0%
!! ATAGCGATATTTAGGTTATA+GGG + chr4.1:1814825-1814844 MS.gene052666:intron 25.0%
!! ATATGACATTGTCCAATTTT+TGG - chr4.1:1814137-1814156 None:intergenic 25.0%
!! CTGAAGATATATTTTCATTG+AGG + chr4.1:1814301-1814320 MS.gene052666:intron 25.0%
!! GTGCTAATTTGCAAATATAA+AGG - chr4.1:1814653-1814672 None:intergenic 25.0%
!! TTTGATTTCAACTTGGTTAA+AGG - chr4.1:1815103-1815122 None:intergenic 25.0%
!!! AATTTGGAATTGAATTTTGC+AGG + chr4.1:1813736-1813755 MS.gene052666:intron 25.0%
!!! CTCTTGTTGATTATTGATTT+TGG + chr4.1:1814269-1814288 MS.gene052666:intron 25.0%
!!! GAAATTGATTGCTGTTTTTA+GGG + chr4.1:1814327-1814346 MS.gene052666:intron 25.0%
!!! TTGTTGATTATTGATTTTGG+TGG + chr4.1:1814272-1814291 MS.gene052666:intron 25.0%
AAGGCATATAACCTTCAAAA+TGG - chr4.1:1814496-1814515 None:intergenic 30.0%
AATGATCATGTTACGAAGAT+GGG - chr4.1:1815034-1815053 None:intergenic 30.0%
ATTTCCGATAGAATCTAACA+TGG + chr4.1:1813696-1813715 MS.gene052666:intron 30.0%
CTTACTGAATAGCGATATTT+AGG + chr4.1:1814817-1814836 MS.gene052666:intron 30.0%
CTTCCATGTAAACTTGAAAA+AGG - chr4.1:1814515-1814534 None:intergenic 30.0%
GTTATAGGGACTAAATTGAT+TGG + chr4.1:1814839-1814858 MS.gene052666:intron 30.0%
TGTGAATTGGTTGTAGAAAA+GGG + chr4.1:1814972-1814991 MS.gene052666:intron 30.0%
TTCTTGAATGTTCTCTTCTT+CGG - chr4.1:1813803-1813822 None:intergenic 30.0%
TTGTGAATTGGTTGTAGAAA+AGG + chr4.1:1814971-1814990 MS.gene052666:intron 30.0%
! GACTTTAGTACCCATTTTAA+TGG + chr4.1:1814186-1814205 MS.gene052666:intron 30.0%
!!! ATGCCTTTTTCAAGTTTACA+TGG + chr4.1:1814509-1814528 MS.gene052666:intron 30.0%
!!! CGAAATTGATTGCTGTTTTT+AGG + chr4.1:1814326-1814345 MS.gene052666:intron 30.0%
AGCATTATTGGAGTGTACAA+TGG + chr4.1:1815072-1815091 MS.gene052666:CDS 35.0%
ATAGGGACTAAATTGATTGG+TGG + chr4.1:1814842-1814861 MS.gene052666:intron 35.0%
ATCATTGTACCTGAGATGAT+TGG + chr4.1:1815048-1815067 MS.gene052666:CDS 35.0%
ATCGGAAATCAAGTTTCAGA+AGG - chr4.1:1813685-1813704 None:intergenic 35.0%
CAATGATCATGTTACGAAGA+TGG - chr4.1:1815035-1815054 None:intergenic 35.0%
GAAATCAAACCTGAGATGAT+CGG + chr4.1:1815114-1815133 MS.gene052666:CDS 35.0%
GCATATAACCTTCAAAATGG+TGG - chr4.1:1814493-1814512 None:intergenic 35.0%
GCATTATTGGAGTGTACAAT+GGG + chr4.1:1815073-1815092 MS.gene052666:CDS 35.0%
GTGACTCAGTGTTCAAATTA+AGG - chr4.1:1814683-1814702 None:intergenic 35.0%
TCATTGTACCTGAGATGATT+GGG + chr4.1:1815049-1815068 MS.gene052666:CDS 35.0%
TTCAAGAAGTTCAGTTTCAG+AGG + chr4.1:1813815-1813834 MS.gene052666:CDS 35.0%
! ACGCATGTAAACCTTTAGAA+TGG - chr4.1:1814736-1814755 None:intergenic 35.0%
! CAAAGTTACCACCATTTTGA+AGG + chr4.1:1814482-1814501 MS.gene052666:intron 35.0%
! TCTCAGGTTTGATTTCAACT+TGG - chr4.1:1815110-1815129 None:intergenic 35.0%
ATAATGCTCCCAATCATCTC+AGG - chr4.1:1815060-1815079 None:intergenic 40.0%
ATGAGATCGAGAATTCAGCT+AGG - chr4.1:1815146-1815165 None:intergenic 40.0%
CATCAAAAAGCTTCGCAAAG+CGG + chr4.1:1813949-1813968 MS.gene052666:CDS 40.0%
GAATTGGTTGTAGAAAAGGG+AGG + chr4.1:1814975-1814994 MS.gene052666:intron 40.0%
TCATACAAGCCTGTTAAGCA+TGG + chr4.1:1815162-1815181 MS.gene052666:CDS 40.0%
! AAGCTTTTTGATGAGAGCCA+TGG - chr4.1:1813942-1813961 None:intergenic 40.0%
! AGCTTTTTGATGAGAGCCAT+GGG - chr4.1:1813941-1813960 None:intergenic 40.0%
! CAGTTTCAGAGGAGTTGATT+TGG + chr4.1:1813826-1813845 MS.gene052666:CDS 40.0%
!! GAGATGATTGGGAGCATTAT+TGG + chr4.1:1815060-1815079 MS.gene052666:CDS 40.0%
AAGATGGGTCCTAACAGGTT+CGG - chr4.1:1815019-1815038 None:intergenic 45.0%
AAGCATGGTAGACCTGGTAT+TGG + chr4.1:1815177-1815196 MS.gene052666:CDS 45.0%
AGGTAATGACCGATCATCTC+AGG - chr4.1:1815126-1815145 None:intergenic 45.0%
GAGCTTAACAAGCTCATCAG+TGG - chr4.1:1813870-1813889 None:intergenic 45.0%
TCACTTGAGAGGAATGAACC+TGG - chr4.1:1815217-1815236 None:intergenic 45.0%
TTACGAAGATGGGTCCTAAC+AGG - chr4.1:1815024-1815043 None:intergenic 45.0%
! AATTTTGCAGGCAGACGTTG+AGG + chr4.1:1813748-1813767 MS.gene052666:intron 45.0%
!! ATAAATAAAAGAAATAAAAA+TGG - chr4.1:1813990-1814009 None:intergenic 5.0%
!! TAAATAAAAGAAATAAAAAT+GGG - chr4.1:1813989-1814008 None:intergenic 5.0%
AGATGGGTCCTAACAGGTTC+GGG - chr4.1:1815018-1815037 None:intergenic 50.0%
CCAGGTCTACCATGCTTAAC+AGG - chr4.1:1815174-1815193 None:intergenic 50.0%
CCTGTTAAGCATGGTAGACC+TGG + chr4.1:1815171-1815190 MS.gene052666:CDS 50.0%
CTTGAGAGGAATGAACCTGG+AGG - chr4.1:1815214-1815233 None:intergenic 50.0%
GAGTGAGTAGCACCAATACC+AGG - chr4.1:1815192-1815211 None:intergenic 50.0%
AGGTCTCACTCGTAAGCCCA+TGG + chr4.1:1813922-1813941 MS.gene052666:CDS 55.0%
GCTCGTAGAAGGTTCCAGAG+AGG + chr4.1:1813902-1813921 MS.gene052666:CDS 55.0%
GCTTACGAGTGAGACCTCTC+TGG - chr4.1:1813919-1813938 None:intergenic 55.0%
GTGAGAAGCCCGAACCTGTT+AGG + chr4.1:1815007-1815026 MS.gene052666:CDS 55.0%
TCAGTGCTCGTGCTCGTAGA+AGG + chr4.1:1813891-1813910 MS.gene052666:CDS 55.0%
TCTCTTCTTCGGTACTCCCG+CGG - chr4.1:1813792-1813811 None:intergenic 55.0%
!! TAGAAAAGGGAGGCACCACC+TGG + chr4.1:1814985-1815004 MS.gene052666:intron 55.0%
!! TTGGTGCTACTCACTCCTCC+AGG + chr4.1:1815196-1815215 MS.gene052666:CDS 55.0%
ACAGGTTCGGGCTTCTCACC+AGG - chr4.1:1815006-1815025 None:intergenic 60.0%
CTTCTTCGGTACTCCCGCGG+CGG - chr4.1:1813789-1813808 None:intergenic 65.0%
GGTTCGGGCTTCTCACCAGG+TGG - chr4.1:1815003-1815022 None:intergenic 65.0%
AGATGCAGCAGCCGCCGCCG+CGG + chr4.1:1813772-1813791 MS.gene052666:CDS 75.0%
CTTCGGTACTCCCGCGGCGG+CGG - chr4.1:1813786-1813805 None:intergenic 75.0%
! GATGCAGCAGCCGCCGCCGC+GGG + chr4.1:1813773-1813792 MS.gene052666:CDS 80.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 1813666 1815236 1813666 ID=MS.gene052666
chr4.1 mRNA 1813666 1815236 1813666 ID=MS.gene052666.t1;Parent=MS.gene052666
chr4.1 exon 1813666 1813668 1813666 ID=MS.gene052666.t1.exon1;Parent=MS.gene052666.t1
chr4.1 CDS 1813666 1813668 1813666 ID=cds.MS.gene052666.t1;Parent=MS.gene052666.t1
chr4.1 exon 1813758 1813970 1813758 ID=MS.gene052666.t1.exon2;Parent=MS.gene052666.t1
chr4.1 CDS 1813758 1813970 1813758 ID=cds.MS.gene052666.t1;Parent=MS.gene052666.t1
chr4.1 exon 1814988 1815236 1814988 ID=MS.gene052666.t1.exon3;Parent=MS.gene052666.t1
chr4.1 CDS 1814988 1815236 1814988 ID=cds.MS.gene052666.t1;Parent=MS.gene052666.t1
Gene Sequence

>MS.gene052666

ATGGTGAGTTAACAATCCTTCTGAAACTTGATTTCCGATAGAATCTAACATGGATTTCCGATTGAATTTGAATTTGGAATTGAATTTTGCAGGCAGACGTTGAGGTAGATGCAGCAGCCGCCGCCGCGGGAGTACCGAAGAAGAGAACATTCAAGAAGTTCAGTTTCAGAGGAGTTGATTTGGATGCACTTCTTGACATGTCCACTGATGAGCTTGTTAAGCTCTTCAGTGCTCGTGCTCGTAGAAGGTTCCAGAGAGGTCTCACTCGTAAGCCCATGGCTCTCATCAAAAAGCTTCGCAAAGCGGTACAATTTTATTTTCCCATTTTTATTTCTTTTATTTATCTGAAAATTAAATAGTAAAATTAAAACAACAATCTTAGCATTTTGGGCGTGCTCAATTTAGTTCCTTTAATATGCAAAATACTAACAATTGGGTTCCTTAAATTTGTAAAATAGTAATTGCATACCAAAAATTGGACAATGTCATATCAGTCATTAGTATTTTTGTGTTTTTGTCCGACTTTAGTACCCATTTTAATGGTTTCATGTTCATTTTGATGCAATAATTGCTTCTAGAACATATTCAAGTTACTATTCAAGTCTCTTGTTGATTATTGATTTTGGTGGTTTGTTCTGAAGATATATTTTCATTGAGGTGCGAAATTGATTGCTGTTTTTAGGGATTAATTTGTTATTTTGTTAATTTGAGGCATTAAATTGCTCAAACTTTACCAATATATCAATTTAGTTCCTTAAATTTGCATAATAGGTAATTTAGTCCCTTAAATTGAACACTTTTTTTTTTTTTTTGGTGCAAAGTTACCACCATTTTGAAGGTTATATGCCTTTTTCAAGTTTACATGGAAGTTTTTATTGATTTTGTTGGATCTTTACTAAAGTTATATTTTGCTTGAAGAACTTAGTTATTTTGTTATTCTTTTATTTAGTGGTTAATTGAATTTCCAAAAATTTTGATGTTAGTCCTTTATATTTGCAAATTAGCACTTTAGTACCTTAATTTGAACACTGAGTCACTTTTTTTTTTTTGCTTTAAATTTAACACTACCATTCTAAAGGTTTACATGCGTCAAATGCATCAAGAGACTAGTTAAGTTAATATTTAAACAATTTTATTGATTTTGATGAATGCTTACTGAATAGCGATATTTAGGTTATAGGGACTAAATTGATTGGTGGTTTTTTAAATTTAAAGGACTACTTTGTTAATTTGTGAATTTGAGGGACTAAATTACTTGACCCTTACTATCTTAAGTACTAAATGCTGATTCATATTGTTATAATTTTGTGAATTGGTTGTAGAAAAGGGAGGCACCACCTGGTGAGAAGCCCGAACCTGTTAGGACCCATCTTCGTAACATGATCATTGTACCTGAGATGATTGGGAGCATTATTGGAGTGTACAATGGGAAGACCTTTAACCAAGTTGAAATCAAACCTGAGATGATCGGTCATTACCTAGCTGAATTCTCGATCTCATACAAGCCTGTTAAGCATGGTAGACCTGGTATTGGTGCTACTCACTCCTCCAGGTTCATTCCTCTCAAGTGA

Protein sequence

>MS.gene052666.t1

MADVEVDAAAAAAGVPKKRTFKKFSFRGVDLDALLDMSTDELVKLFSARARRRFQRGLTRKPMALIKKLRKAKREAPPGEKPEPVRTHLRNMIIVPEMIGSIIGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK