Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72110.t1 | XP_003608796.1 | 94.3 | 123 | 7 | 0 | 1 | 123 | 1 | 123 | 5.60E-56 | 226.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72110.t1 | G7JK81 | 94.3 | 123 | 7 | 0 | 1 | 123 | 1 | 123 | 4.0e-56 | 226.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054713 | MS.gene72110 | 0.885157 | 1.04E-71 | -1.69E-46 |
| MS.gene055860 | MS.gene72110 | 0.810787 | 9.69E-51 | -1.69E-46 |
| MS.gene058042 | MS.gene72110 | 0.881156 | 3.03E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72110.t1 | MTR_4g102040 | 94.309 | 123 | 7 | 0 | 1 | 123 | 1 | 123 | 4.17e-82 | 238 |
| MS.gene72110.t1 | MTR_5g011750 | 62.500 | 88 | 28 | 1 | 41 | 123 | 5 | 92 | 6.74e-31 | 107 |
| MS.gene72110.t1 | MTR_4g055420 | 53.623 | 69 | 32 | 0 | 57 | 125 | 69 | 137 | 2.49e-19 | 79.7 |
| MS.gene72110.t1 | MTR_2g084805 | 50.746 | 67 | 33 | 0 | 57 | 123 | 57 | 123 | 1.30e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72110.t1 | AT2G27830 | 52.239 | 67 | 32 | 0 | 57 | 123 | 68 | 134 | 6.89e-18 | 75.9 |
Find 30 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCAGTGTTCTTCAAAAT+TGG | 0.195240 | 8.1:-21690552 | None:intergenic |
| GTTTCTCTGGCTGTGAATTA+TGG | 0.269497 | 8.1:-21690320 | None:intergenic |
| CATTACTTTGGAAAGTGAAT+TGG | 0.312890 | 8.1:-21690599 | None:intergenic |
| AAACTAATGACACTGTTCTT+AGG | 0.355610 | 8.1:+21690397 | MS.gene72110:CDS |
| ATTACTTTGGAAAGTGAATT+GGG | 0.392489 | 8.1:-21690598 | None:intergenic |
| CACCGTTGAAGATTTGATAA+AGG | 0.392539 | 8.1:+21690494 | MS.gene72110:CDS |
| GCTGTGAATTATGGAGTTTA+CGG | 0.413396 | 8.1:-21690311 | None:intergenic |
| TTAGTAAGATGTCATTACTT+TGG | 0.418927 | 8.1:-21690611 | None:intergenic |
| AGAATTCAACGGGGTCTTAC+CGG | 0.420392 | 8.1:-21690286 | None:intergenic |
| GTTTACGGCAAGAATTCAAC+GGG | 0.423723 | 8.1:-21690296 | None:intergenic |
| ACATTGCCAGAGGGTTTCTC+TGG | 0.447363 | 8.1:-21690333 | None:intergenic |
| GTGACAATCGAAAACAGCTT+GGG | 0.499309 | 8.1:+21690441 | MS.gene72110:CDS |
| AAGCATGACTTCTCCGCCAC+AGG | 0.512058 | 8.1:+21690245 | None:intergenic |
| CTTCTCCGCCACAGGCTCAT+CGG | 0.524598 | 8.1:+21690253 | MS.gene72110:CDS |
| GGTGACAATCGAAAACAGCT+TGG | 0.547560 | 8.1:+21690440 | MS.gene72110:CDS |
| GTTTGCATCTTCAGCGTCAG+AGG | 0.563732 | 8.1:-21690378 | None:intergenic |
| TGCCTTTATCAAATCTTCAA+CGG | 0.571891 | 8.1:-21690496 | None:intergenic |
| AGATTTGATAAAGGCAGCGT+TGG | 0.574906 | 8.1:+21690503 | MS.gene72110:CDS |
| CCTCTGGCAATGTTGATCGT+CGG | 0.591836 | 8.1:+21690343 | MS.gene72110:CDS |
| TCACAGCCAGAGAAACCCTC+TGG | 0.594528 | 8.1:+21690327 | MS.gene72110:CDS |
| TTTCTCCGATGAGCCTGTGG+CGG | 0.602364 | 8.1:-21690258 | None:intergenic |
| GCCATCAAAACTGCTACTGA+AGG | 0.608756 | 8.1:+21690419 | MS.gene72110:CDS |
| AGTTTACGGCAAGAATTCAA+CGG | 0.621562 | 8.1:-21690297 | None:intergenic |
| TAATGAAGATCATAACACCT+TGG | 0.638724 | 8.1:-21690573 | None:intergenic |
| ATTCAACGGGGTCTTACCGG+AGG | 0.655189 | 8.1:-21690283 | None:intergenic |
| CGACGATCAACATTGCCAGA+GGG | 0.655617 | 8.1:-21690342 | None:intergenic |
| TCAGATGTTGATGTTGTCTG+AGG | 0.666940 | 8.1:+21690470 | MS.gene72110:CDS |
| TTTATGAGAAGGATAAGAGG+AGG | 0.687109 | 8.1:+21690529 | MS.gene72110:CDS |
| CCGACGATCAACATTGCCAG+AGG | 0.706693 | 8.1:-21690343 | None:intergenic |
| TTTACGGCAAGAATTCAACG+GGG | 0.837986 | 8.1:-21690295 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTAGTAAGATGTCATTACTT+TGG | - | chr8.1:21690614-21690633 | None:intergenic | 25.0% |
| !! | ATTACTTTGGAAAGTGAATT+GGG | - | chr8.1:21690601-21690620 | None:intergenic | 25.0% |
| AAACTAATGACACTGTTCTT+AGG | + | chr8.1:21690397-21690416 | MS.gene72110:CDS | 30.0% | |
| TAATGAAGATCATAACACCT+TGG | - | chr8.1:21690576-21690595 | None:intergenic | 30.0% | |
| TGCCTTTATCAAATCTTCAA+CGG | - | chr8.1:21690499-21690518 | None:intergenic | 30.0% | |
| ! | CATTACTTTGGAAAGTGAAT+TGG | - | chr8.1:21690602-21690621 | None:intergenic | 30.0% |
| ! | TGTTTTATGAGAAGGATAAG+AGG | + | chr8.1:21690526-21690545 | MS.gene72110:CDS | 30.0% |
| AGTTTACGGCAAGAATTCAA+CGG | - | chr8.1:21690300-21690319 | None:intergenic | 35.0% | |
| CACCGTTGAAGATTTGATAA+AGG | + | chr8.1:21690494-21690513 | MS.gene72110:CDS | 35.0% | |
| GCTGTGAATTATGGAGTTTA+CGG | - | chr8.1:21690314-21690333 | None:intergenic | 35.0% | |
| GGATCAGTGTTCTTCAAAAT+TGG | - | chr8.1:21690555-21690574 | None:intergenic | 35.0% | |
| TTTATGAGAAGGATAAGAGG+AGG | + | chr8.1:21690529-21690548 | MS.gene72110:CDS | 35.0% | |
| !! | TTTTGAAGAACACTGATCCA+AGG | + | chr8.1:21690556-21690575 | MS.gene72110:CDS | 35.0% |
| AGATTTGATAAAGGCAGCGT+TGG | + | chr8.1:21690503-21690522 | MS.gene72110:CDS | 40.0% | |
| GTGACAATCGAAAACAGCTT+GGG | + | chr8.1:21690441-21690460 | MS.gene72110:CDS | 40.0% | |
| GTTTACGGCAAGAATTCAAC+GGG | - | chr8.1:21690299-21690318 | None:intergenic | 40.0% | |
| GTTTCTCTGGCTGTGAATTA+TGG | - | chr8.1:21690323-21690342 | None:intergenic | 40.0% | |
| TCAGATGTTGATGTTGTCTG+AGG | + | chr8.1:21690470-21690489 | MS.gene72110:CDS | 40.0% | |
| TTTACGGCAAGAATTCAACG+GGG | - | chr8.1:21690298-21690317 | None:intergenic | 40.0% | |
| !! | ACCTTCAGTAGCAGTTTTGA+TGG | - | chr8.1:21690423-21690442 | None:intergenic | 40.0% |
| !!! | AGCGTTGGTGTTTTATGAGA+AGG | + | chr8.1:21690518-21690537 | MS.gene72110:CDS | 40.0% |
| AGAATTCAACGGGGTCTTAC+CGG | - | chr8.1:21690289-21690308 | None:intergenic | 45.0% | |
| GCCATCAAAACTGCTACTGA+AGG | + | chr8.1:21690419-21690438 | MS.gene72110:CDS | 45.0% | |
| GGTGACAATCGAAAACAGCT+TGG | + | chr8.1:21690440-21690459 | MS.gene72110:CDS | 45.0% | |
| ACATTGCCAGAGGGTTTCTC+TGG | - | chr8.1:21690336-21690355 | None:intergenic | 50.0% | |
| CCTCTGGCAATGTTGATCGT+CGG | + | chr8.1:21690343-21690362 | MS.gene72110:CDS | 50.0% | |
| CGACGATCAACATTGCCAGA+GGG | - | chr8.1:21690345-21690364 | None:intergenic | 50.0% | |
| GCTCATCGGAGAAAAACCTC+CGG | + | chr8.1:21690267-21690286 | MS.gene72110:CDS | 50.0% | |
| GTTTGCATCTTCAGCGTCAG+AGG | - | chr8.1:21690381-21690400 | None:intergenic | 50.0% | |
| ! | GTTTTTCTCCGATGAGCCTG+TGG | - | chr8.1:21690264-21690283 | None:intergenic | 50.0% |
| ATTCAACGGGGTCTTACCGG+AGG | - | chr8.1:21690286-21690305 | None:intergenic | 55.0% | |
| CCGACGATCAACATTGCCAG+AGG | - | chr8.1:21690346-21690365 | None:intergenic | 55.0% | |
| TCACAGCCAGAGAAACCCTC+TGG | + | chr8.1:21690327-21690346 | MS.gene72110:CDS | 55.0% | |
| TTTCTCCGATGAGCCTGTGG+CGG | - | chr8.1:21690261-21690280 | None:intergenic | 55.0% | |
| CTTCTCCGCCACAGGCTCAT+CGG | + | chr8.1:21690253-21690272 | MS.gene72110:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 21690249 | 21690647 | 21690249 | ID=MS.gene72110 |
| chr8.1 | mRNA | 21690249 | 21690647 | 21690249 | ID=MS.gene72110.t1;Parent=MS.gene72110 |
| chr8.1 | exon | 21690249 | 21690647 | 21690249 | ID=MS.gene72110.t1.exon1;Parent=MS.gene72110.t1 |
| chr8.1 | CDS | 21690249 | 21690647 | 21690249 | ID=cds.MS.gene72110.t1;Parent=MS.gene72110.t1 |
| Gene Sequence |
| Protein sequence |
