Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055860.t1 | XP_013465033.1 | 94.2 | 502 | 28 | 1 | 1 | 502 | 1 | 501 | 7.70E-255 | 889.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055860.t1 | Q8S8I1 | 37.7 | 366 | 191 | 8 | 150 | 479 | 101 | 465 | 2.9e-53 | 211.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055860.t1 | A0A072VC90 | 94.2 | 502 | 28 | 1 | 1 | 502 | 1 | 501 | 5.5e-255 | 889.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene055860 | 0.810474 | 1.13E-50 | -1.69E-46 |
| MS.gene054713 | MS.gene055860 | 0.824152 | 9.42E-54 | -1.69E-46 |
| MS.gene055860 | MS.gene058042 | 0.823938 | 1.06E-53 | -1.69E-46 |
| MS.gene055860 | MS.gene058168 | 0.818554 | 1.84E-52 | -1.69E-46 |
| MS.gene055860 | MS.gene065054 | 0.810082 | 1.38E-50 | -1.69E-46 |
| MS.gene055860 | MS.gene065988 | 0.838154 | 3.40E-57 | -1.69E-46 |
| MS.gene055860 | MS.gene066092 | 0.824217 | 9.10E-54 | -1.69E-46 |
| MS.gene055860 | MS.gene066094 | 0.805832 | 1.10E-49 | -1.69E-46 |
| MS.gene055860 | MS.gene066098 | 0.808983 | 2.37E-50 | -1.69E-46 |
| MS.gene055860 | MS.gene069088 | 0.828683 | 7.85E-55 | -1.69E-46 |
| MS.gene055860 | MS.gene26752 | 0.808946 | 2.41E-50 | -1.69E-46 |
| MS.gene055860 | MS.gene27868 | 0.82803 | 1.13E-54 | -1.69E-46 |
| MS.gene055860 | MS.gene31520 | 0.871784 | 5.25E-67 | -1.69E-46 |
| MS.gene055860 | MS.gene36896 | 0.811129 | 8.17E-51 | -1.69E-46 |
| MS.gene055860 | MS.gene38772 | 0.829879 | 4.02E-55 | -1.69E-46 |
| MS.gene055860 | MS.gene43800 | 0.819463 | 1.15E-52 | -1.69E-46 |
| MS.gene055860 | MS.gene44897 | 0.810781 | 9.72E-51 | -1.69E-46 |
| MS.gene055860 | MS.gene46374 | 0.800655 | 1.30E-48 | -1.69E-46 |
| MS.gene055860 | MS.gene47283 | 0.806287 | 8.85E-50 | -1.69E-46 |
| MS.gene055860 | MS.gene49839 | 0.81882 | 1.61E-52 | -1.69E-46 |
| MS.gene055860 | MS.gene51052 | 0.810998 | 8.72E-51 | -1.69E-46 |
| MS.gene055860 | MS.gene51055 | 0.806428 | 8.27E-50 | -1.69E-46 |
| MS.gene055860 | MS.gene55625 | 0.803179 | 3.94E-49 | -1.69E-46 |
| MS.gene055860 | MS.gene56057 | 0.81701 | 4.12E-52 | -1.69E-46 |
| MS.gene055860 | MS.gene59355 | 0.850751 | 1.39E-60 | -1.69E-46 |
| MS.gene055860 | MS.gene59442 | 0.834744 | 2.51E-56 | -1.69E-46 |
| MS.gene055860 | MS.gene64425 | 0.800921 | 1.15E-48 | -1.69E-46 |
| MS.gene055860 | MS.gene64740 | 0.808785 | 2.61E-50 | -1.69E-46 |
| MS.gene055860 | MS.gene68036 | 0.838969 | 2.10E-57 | -1.69E-46 |
| MS.gene055860 | MS.gene68582 | 0.831137 | 1.98E-55 | -1.69E-46 |
| MS.gene055860 | MS.gene72110 | 0.810787 | 9.69E-51 | -1.69E-46 |
| MS.gene055860 | MS.gene73257 | 0.841281 | 5.23E-58 | -1.69E-46 |
| MS.gene055860 | MS.gene74741 | 0.81513 | 1.08E-51 | -1.69E-46 |
| MS.gene055860 | MS.gene74971 | 0.829054 | 6.38E-55 | -1.69E-46 |
| MS.gene055860 | MS.gene76226 | 0.809879 | 1.52E-50 | -1.69E-46 |
| MS.gene055860 | MS.gene76373 | 0.820746 | 5.83E-53 | -1.69E-46 |
| MS.gene055860 | MS.gene82810 | 0.802769 | 4.79E-49 | -1.69E-46 |
| MS.gene055860 | MS.gene87430 | 0.822798 | 1.95E-53 | -1.69E-46 |
| MS.gene055860 | MS.gene87897 | 0.828124 | 1.07E-54 | -1.69E-46 |
| MS.gene055860 | MS.gene88788 | 0.812168 | 4.86E-51 | -1.69E-46 |
| MS.gene055860 | MS.gene90151 | 0.801714 | 7.90E-49 | -1.69E-46 |
| MS.gene055860 | MS.gene92590 | 0.834936 | 2.25E-56 | -1.69E-46 |
| MS.gene055860 | MS.gene98071 | 0.826656 | 2.41E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055860.t1 | MTR_2g090050 | 94.223 | 502 | 28 | 1 | 1 | 502 | 1 | 501 | 0.0 | 939 |
| MS.gene055860.t1 | MTR_1g106735 | 28.807 | 243 | 169 | 3 | 245 | 484 | 369 | 610 | 4.63e-26 | 112 |
| MS.gene055860.t1 | MTR_4g130490 | 32.444 | 225 | 135 | 3 | 265 | 485 | 399 | 610 | 1.42e-24 | 107 |
| MS.gene055860.t1 | MTR_1g106735 | 28.689 | 244 | 169 | 4 | 245 | 484 | 369 | 611 | 1.56e-24 | 107 |
| MS.gene055860.t1 | MTR_1g018440 | 28.571 | 350 | 216 | 8 | 150 | 492 | 299 | 621 | 3.38e-24 | 106 |
| MS.gene055860.t1 | MTR_4g130490 | 33.333 | 204 | 119 | 3 | 265 | 464 | 399 | 589 | 1.80e-21 | 98.2 |
| MS.gene055860.t1 | MTR_2g028220 | 27.039 | 233 | 161 | 1 | 252 | 484 | 257 | 480 | 1.90e-20 | 94.7 |
| MS.gene055860.t1 | MTR_4g130490 | 34.247 | 146 | 79 | 3 | 265 | 406 | 399 | 531 | 3.57e-14 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055860.t1 | AT2G20815 | 37.568 | 370 | 192 | 9 | 150 | 482 | 101 | 468 | 1.21e-70 | 233 |
| MS.gene055860.t1 | AT2G20815 | 37.466 | 371 | 192 | 10 | 150 | 482 | 72 | 440 | 4.61e-69 | 228 |
| MS.gene055860.t1 | AT2G20815 | 37.466 | 371 | 192 | 10 | 150 | 482 | 101 | 469 | 5.91e-69 | 229 |
| MS.gene055860.t1 | AT2G20815 | 32.961 | 358 | 177 | 9 | 150 | 482 | 72 | 391 | 1.29e-52 | 183 |
| MS.gene055860.t1 | AT2G24070 | 33.333 | 219 | 136 | 1 | 265 | 483 | 376 | 584 | 4.37e-28 | 118 |
| MS.gene055860.t1 | AT2G24070 | 33.333 | 219 | 136 | 1 | 265 | 483 | 376 | 584 | 4.37e-28 | 118 |
| MS.gene055860.t1 | AT2G24070 | 33.333 | 219 | 136 | 1 | 265 | 483 | 376 | 584 | 4.37e-28 | 118 |
| MS.gene055860.t1 | AT4G30710 | 31.507 | 219 | 140 | 1 | 265 | 483 | 412 | 620 | 9.02e-28 | 117 |
| MS.gene055860.t1 | AT4G30710 | 31.507 | 219 | 140 | 1 | 265 | 483 | 412 | 620 | 9.02e-28 | 117 |
| MS.gene055860.t1 | AT4G30710 | 31.507 | 219 | 140 | 1 | 265 | 483 | 412 | 620 | 3.16e-27 | 115 |
| MS.gene055860.t1 | AT4G30710 | 31.429 | 210 | 134 | 1 | 265 | 474 | 412 | 611 | 2.82e-25 | 110 |
| MS.gene055860.t1 | AT4G25190 | 27.007 | 274 | 170 | 4 | 239 | 489 | 128 | 394 | 9.81e-24 | 103 |
| MS.gene055860.t1 | AT4G25190 | 26.909 | 275 | 170 | 5 | 239 | 489 | 128 | 395 | 2.84e-22 | 99.4 |
| MS.gene055860.t1 | AT1G49890 | 30.729 | 192 | 133 | 0 | 293 | 484 | 455 | 646 | 3.42e-22 | 100 |
| MS.gene055860.t1 | AT3G19570 | 28.646 | 192 | 137 | 0 | 293 | 484 | 450 | 641 | 1.13e-21 | 99.0 |
| MS.gene055860.t1 | AT3G19570 | 30.233 | 172 | 120 | 0 | 293 | 464 | 450 | 621 | 1.44e-18 | 89.4 |
| MS.gene055860.t1 | AT3G60000 | 28.351 | 194 | 135 | 2 | 292 | 483 | 250 | 441 | 3.33e-18 | 87.4 |
| MS.gene055860.t1 | AT5G43160 | 27.778 | 180 | 130 | 0 | 306 | 485 | 82 | 261 | 4.49e-18 | 84.3 |
| MS.gene055860.t1 | AT5G43160 | 27.778 | 180 | 130 | 0 | 306 | 485 | 82 | 261 | 4.49e-18 | 84.3 |
| MS.gene055860.t1 | AT2G44190 | 28.856 | 201 | 139 | 2 | 287 | 485 | 264 | 462 | 2.42e-17 | 85.1 |
| MS.gene055860.t1 | AT3G60000 | 29.586 | 169 | 115 | 2 | 292 | 458 | 250 | 416 | 7.28e-16 | 80.1 |
| MS.gene055860.t1 | AT3G19570 | 31.724 | 145 | 99 | 0 | 293 | 437 | 450 | 594 | 4.70e-14 | 75.1 |
| MS.gene055860.t1 | AT3G19570 | 31.724 | 145 | 99 | 0 | 293 | 437 | 450 | 594 | 4.70e-14 | 75.1 |
Find 102 sgRNAs with CRISPR-Local
Find 145 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGTTAACATTGACTTAA+TGG | 0.211672 | 2.1:+11387124 | None:intergenic |
| AACCATTCGACGGCGAATTA+AGG | 0.246819 | 2.1:+11388172 | None:intergenic |
| TCATCCAAGGAAAACCTTCC+TGG | 0.274888 | 2.1:+11388558 | None:intergenic |
| AATTCGCCGTCGAATGGTTT+TGG | 0.279413 | 2.1:-11388168 | MS.gene055860:CDS |
| AAGAAGCTTAATTCTAATAA+TGG | 0.280763 | 2.1:-11388594 | MS.gene055860:CDS |
| TGTGTCTCCTATCGTCTTCC+TGG | 0.281185 | 2.1:+11388880 | None:intergenic |
| GTTAAAGTTATTAGAAGCTT+GGG | 0.334551 | 2.1:-11387531 | MS.gene055860:CDS |
| ACAAGAGCAATTGGTGGTTC+TGG | 0.341427 | 2.1:-11388654 | MS.gene055860:CDS |
| CTTAATTCTAATAATGGTTC+AGG | 0.357073 | 2.1:-11388588 | MS.gene055860:CDS |
| CAATGAAGCTTTGGCTTCTC+CGG | 0.358325 | 2.1:-11388475 | MS.gene055860:CDS |
| AGGAAATTCAGTGGATTCTA+TGG | 0.360707 | 2.1:+11388941 | None:intergenic |
| CATAACATTATTTCTGAACT+TGG | 0.360742 | 2.1:+11387712 | None:intergenic |
| TTGTTCTATAATTCTATCTT+CGG | 0.365225 | 2.1:+11388770 | None:intergenic |
| AGCAGAATGCAAACACTCTT+TGG | 0.368717 | 2.1:+11387469 | None:intergenic |
| AATGGTTCAGGCATTGTTCC+AGG | 0.369988 | 2.1:-11388576 | MS.gene055860:CDS |
| GCCTGGTTCAATTTGAAGAT+TGG | 0.377854 | 2.1:-11386690 | MS.gene055860:CDS |
| CAAGTCATCGATTCGGAGCC+AGG | 0.381540 | 2.1:-11388898 | MS.gene055860:CDS |
| CAAAGCTTCATTGTTGGATT+CGG | 0.388717 | 2.1:+11388485 | None:intergenic |
| TTCAACTCCTTTCGGCTTCG+TGG | 0.391246 | 2.1:+11388245 | None:intergenic |
| ACTGCTAGGGATTACTTCAT+AGG | 0.393630 | 2.1:-11386641 | None:intergenic |
| TCAACTCCTTTCGGCTTCGT+GGG | 0.401313 | 2.1:+11388246 | None:intergenic |
| TACAGCCATTGCTATTCGTC+AGG | 0.408751 | 2.1:-11387165 | MS.gene055860:CDS |
| AATGATCAAACAAGAGCAAT+TGG | 0.411484 | 2.1:-11388663 | MS.gene055860:CDS |
| TCACAATGGGCTTTGTCGCC+AGG | 0.413929 | 2.1:-11388288 | MS.gene055860:CDS |
| CGAATCCAACAATGAAGCTT+TGG | 0.417224 | 2.1:-11388484 | MS.gene055860:CDS |
| AGTTAAAGTTATTAGAAGCT+TGG | 0.428073 | 2.1:-11387532 | MS.gene055860:CDS |
| AAGAGGACTAATTCCATTAC+GGG | 0.433019 | 2.1:-11388327 | MS.gene055860:CDS |
| TGCTGCAATACTATCAGAAT+TGG | 0.434012 | 2.1:-11386916 | MS.gene055860:CDS |
| AGAGCAATTTGATATGTGTC+CGG | 0.435213 | 2.1:-11387745 | MS.gene055860:CDS |
| GCGTCACTCATCAACCTTGT+AGG | 0.436142 | 2.1:+11388429 | None:intergenic |
| GAAGAGGACTAATTCCATTA+CGG | 0.436247 | 2.1:-11388328 | MS.gene055860:CDS |
| GAAACTTACTGCAGGTGAAA+AGG | 0.439050 | 2.1:+11387103 | None:intergenic |
| CGAACTCTTGTACCCCGTAA+TGG | 0.444702 | 2.1:+11388314 | None:intergenic |
| TGTGTGCAGAGTTCCTCTTT+TGG | 0.450064 | 2.1:-11387447 | MS.gene055860:CDS |
| GGCCTTAATTCGCCGTCGAA+TGG | 0.452120 | 2.1:-11388174 | MS.gene055860:CDS |
| AGAAGCCAAAGCTTCATTGT+TGG | 0.458888 | 2.1:+11388479 | None:intergenic |
| GGAGCTTCTGATTCTAACAT+TGG | 0.470930 | 2.1:-11388384 | MS.gene055860:CDS |
| CTTTGTTGACGAGGGTTCAC+CGG | 0.482670 | 2.1:+11388990 | None:intergenic |
| CTGTTCTACGTCGTCAACTC+TGG | 0.489175 | 2.1:-11388839 | MS.gene055860:CDS |
| CAACTGCAGGATTTACTGCT+AGG | 0.490974 | 2.1:-11386655 | MS.gene055860:CDS |
| GAGCAATTTGATATGTGTCC+GGG | 0.492449 | 2.1:-11387744 | MS.gene055860:CDS |
| TTCTATGGAGGTGGAAGGCG+AGG | 0.496580 | 2.1:+11388956 | None:intergenic |
| TTGATAATCGTTTGATTCAG+TGG | 0.496593 | 2.1:-11388113 | MS.gene055860:CDS |
| TTCAGTGGATTCTATGGAGG+TGG | 0.499093 | 2.1:+11388947 | None:intergenic |
| TTCAATTTGAAGATTGGCAA+AGG | 0.500856 | 2.1:-11386684 | MS.gene055860:CDS |
| CTTGTAGGAACTTCCACCAC+TGG | 0.509361 | 2.1:+11388444 | None:intergenic |
| GGTGGTTCTGGAAGATTCAC+AGG | 0.510011 | 2.1:-11388642 | MS.gene055860:CDS |
| ACCTTAGAACCTTCCAAAAG+AGG | 0.510568 | 2.1:+11387434 | None:intergenic |
| GACTTGCGTATGACGGAGGA+AGG | 0.513844 | 2.1:+11388915 | None:intergenic |
| GCCAATCTTCAAATTGAACC+AGG | 0.515880 | 2.1:+11386689 | None:intergenic |
| GAGCTTCTGATTCTAACATT+GGG | 0.516533 | 2.1:-11388383 | MS.gene055860:CDS |
| CACAATGGGCTTTGTCGCCA+GGG | 0.523874 | 2.1:-11388287 | MS.gene055860:CDS |
| ACATAACATATCTGTTGTTG+AGG | 0.527727 | 2.1:-11386841 | MS.gene055860:intron |
| CTTGAAAACTCTGACCTCCC+TGG | 0.529362 | 2.1:+11388270 | None:intergenic |
| GAATTAGTCCTCTTCATCAT+CGG | 0.532189 | 2.1:+11388336 | None:intergenic |
| ATTAGAAGCTTGGGGAAGCA+TGG | 0.534664 | 2.1:-11387522 | MS.gene055860:CDS |
| CTTGCGTATGACGGAGGAAG+GGG | 0.541168 | 2.1:+11388917 | None:intergenic |
| GAAGTGTTTCGCGCCTTCAT+CGG | 0.542889 | 2.1:+11389014 | None:intergenic |
| CAACTCCTTTCGGCTTCGTG+GGG | 0.543450 | 2.1:+11388247 | None:intergenic |
| CGTTGAAACCGATGATGAAG+AGG | 0.543848 | 2.1:-11388344 | MS.gene055860:CDS |
| CAAGAGTTCGAAATCACAAT+GGG | 0.546027 | 2.1:-11388301 | MS.gene055860:CDS |
| TGAACCCTCGTCAACAAAGA+AGG | 0.550286 | 2.1:-11388986 | MS.gene055860:CDS |
| GCAAAGGAAATATCAACTGC+AGG | 0.551022 | 2.1:-11386668 | MS.gene055860:CDS |
| TCTAACATTGGGAATTTGAA+CGG | 0.552992 | 2.1:-11388372 | MS.gene055860:CDS |
| AACTGCAGGATTTACTGCTA+GGG | 0.555292 | 2.1:-11386654 | MS.gene055860:CDS |
| AGAATGCAAACACTCTTTGG+TGG | 0.563685 | 2.1:+11387472 | None:intergenic |
| TCATACGCAAGTCATCGATT+CGG | 0.566805 | 2.1:-11388905 | MS.gene055860:CDS |
| AATCGATGACTTGCGTATGA+CGG | 0.575103 | 2.1:+11388908 | None:intergenic |
| TTCGGAGCCAGGAAGACGAT+AGG | 0.577435 | 2.1:-11388887 | MS.gene055860:CDS |
| TCCGGCAAGAAAACCAGTGG+TGG | 0.587118 | 2.1:-11388457 | MS.gene055860:CDS |
| GAAACCTTCTTTGTTGACGA+GGG | 0.587157 | 2.1:+11388982 | None:intergenic |
| AAATTCAAGCATAACTAGCT+CGG | 0.589143 | 2.1:-11388514 | MS.gene055860:CDS |
| TGAACTTGGTGAGACCATCC+CGG | 0.589730 | 2.1:+11387726 | None:intergenic |
| AAGCAGTGTAAAGTGCAGCC+TGG | 0.591075 | 2.1:-11386707 | MS.gene055860:CDS |
| CAGTGGTGGAAGTTCCTACA+AGG | 0.591497 | 2.1:-11388443 | MS.gene055860:CDS |
| GAAGGCGCGAAACACTTCTC+CGG | 0.599113 | 2.1:-11389009 | MS.gene055860:CDS |
| TTGTTTCCAAAACCATTCGA+CGG | 0.600083 | 2.1:+11388162 | None:intergenic |
| GTTCAGGCATTGTTCCAGGA+AGG | 0.601143 | 2.1:-11388572 | MS.gene055860:CDS |
| ACTTGCGTATGACGGAGGAA+GGG | 0.602214 | 2.1:+11388916 | None:intergenic |
| AGAAACCTTCTTTGTTGACG+AGG | 0.602654 | 2.1:+11388981 | None:intergenic |
| TCAAGCCCCACGAAGCCGAA+AGG | 0.605749 | 2.1:-11388252 | MS.gene055860:CDS |
| TGAGTGACGCAACATTGAGA+AGG | 0.607970 | 2.1:-11388416 | MS.gene055860:CDS |
| GATCAAACAAGAGCAATTGG+TGG | 0.611838 | 2.1:-11388660 | MS.gene055860:CDS |
| GAGTGACGCAACATTGAGAA+GGG | 0.622733 | 2.1:-11388415 | MS.gene055860:CDS |
| GTGGATTCTATGGAGGTGGA+AGG | 0.624761 | 2.1:+11388951 | None:intergenic |
| CGATGACTTGCGTATGACGG+AGG | 0.625049 | 2.1:+11388911 | None:intergenic |
| AAGCTTGGGGAAGCATGGAA+AGG | 0.627108 | 2.1:-11387517 | MS.gene055860:CDS |
| TTAAAGTTATTAGAAGCTTG+GGG | 0.627151 | 2.1:-11387530 | MS.gene055860:CDS |
| ACAAGAGTTCGAAATCACAA+TGG | 0.644511 | 2.1:-11388302 | MS.gene055860:CDS |
| AATTTGATATGTGTCCGGGA+TGG | 0.647695 | 2.1:-11387740 | MS.gene055860:CDS |
| AGAGGCCTGACGAATAGCAA+TGG | 0.649950 | 2.1:+11387160 | None:intergenic |
| ACATTGAGAAGGGCTAGAAG+AGG | 0.654076 | 2.1:-11388405 | MS.gene055860:CDS |
| TTCTCCGGCAAGAAAACCAG+TGG | 0.654553 | 2.1:-11388460 | MS.gene055860:CDS |
| TCTATGGAGGTGGAAGGCGA+GGG | 0.655607 | 2.1:+11388957 | None:intergenic |
| AAATTCAGTGGATTCTATGG+AGG | 0.656704 | 2.1:+11388944 | None:intergenic |
| CGTATGACGGAGGAAGGGGA+AGG | 0.662645 | 2.1:+11388921 | None:intergenic |
| AATGGAAGCTGTGAGATCAG+AGG | 0.667538 | 2.1:+11387142 | None:intergenic |
| GGAAGGGGAAGGAAATTCAG+TGG | 0.670014 | 2.1:+11388932 | None:intergenic |
| AAACATTTCTCGCCACGCCG+AGG | 0.686205 | 2.1:-11388055 | MS.gene055860:intron |
| AATGGGCTTTGTCGCCAGGG+AGG | 0.703714 | 2.1:-11388284 | MS.gene055860:CDS |
| CAAACAAGTGTACCTCGGCG+TGG | 0.735517 | 2.1:+11388043 | None:intergenic |
| AGAGGACTAATTCCATTACG+GGG | 0.792925 | 2.1:-11388326 | MS.gene055860:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATTACAAAAACAAATT+TGG | + | chr2.1:11388066-11388085 | None:intergenic | 10.0% |
| !!! | AAATCCTTTTAATTTATTTA+TGG | - | chr2.1:11388883-11388902 | MS.gene055860:CDS | 10.0% |
| !!! | TTTAATTTTTGAAATTGTAA+CGG | + | chr2.1:11388337-11388356 | None:intergenic | 10.0% |
| !! | AAATTGAATTAACATTATCT+TGG | - | chr2.1:11387870-11387889 | MS.gene055860:intron | 15.0% |
| !! | AGAAAACAAAAGATATATTT+GGG | + | chr2.1:11388667-11388686 | None:intergenic | 15.0% |
| !! | ATACATATAAAAAGTTTCAA+TGG | + | chr2.1:11388386-11388405 | None:intergenic | 15.0% |
| !! | TCAACCATAAATAAATTAAA+AGG | + | chr2.1:11388890-11388909 | None:intergenic | 15.0% |
| !! | AAGAAGCTTAATTCTAATAA+TGG | - | chr2.1:11387059-11387078 | MS.gene055860:intron | 20.0% |
| !! | CAGAAAACAAAAGATATATT+TGG | + | chr2.1:11388668-11388687 | None:intergenic | 20.0% |
| !! | TTGTTCTATAATTCTATCTT+CGG | + | chr2.1:11386886-11386905 | None:intergenic | 20.0% |
| !!! | AACTGATGTATTGTTATTTT+TGG | - | chr2.1:11388097-11388116 | MS.gene055860:CDS | 20.0% |
| !!! | AATATATCTTTTGTTTTCTG+AGG | - | chr2.1:11388668-11388687 | MS.gene055860:CDS | 20.0% |
| !!! | ATATATCTTTTGTTTTCTGA+GGG | - | chr2.1:11388669-11388688 | MS.gene055860:CDS | 20.0% |
| !!! | GATTTTATCACTATATATGT+TGG | - | chr2.1:11388912-11388931 | MS.gene055860:CDS | 20.0% |
| ! | AATTAGGTTTACTACTTACT+TGG | + | chr2.1:11388029-11388048 | None:intergenic | 25.0% |
| ! | AGTTAAAGTTATTAGAAGCT+TGG | - | chr2.1:11388121-11388140 | MS.gene055860:CDS | 25.0% |
| ! | AGTTTATGCTATAAGATAGT+TGG | - | chr2.1:11387784-11387803 | MS.gene055860:intron | 25.0% |
| ! | ATAACAATACATCAGTTAAG+AGG | + | chr2.1:11388094-11388113 | None:intergenic | 25.0% |
| ! | CTTAATTCTAATAATGGTTC+AGG | - | chr2.1:11387065-11387084 | MS.gene055860:intron | 25.0% |
| ! | GGTAGATGTTAATGAAAATT+AGG | + | chr2.1:11388045-11388064 | None:intergenic | 25.0% |
| ! | GTTAAAGTTATTAGAAGCTT+GGG | - | chr2.1:11388122-11388141 | MS.gene055860:CDS | 25.0% |
| ! | GTTGAAAAACTACTCAATTT+CGG | - | chr2.1:11387425-11387444 | MS.gene055860:intron | 25.0% |
| ! | TGAAAACAACTTACAACTTA+CGG | + | chr2.1:11387674-11387693 | None:intergenic | 25.0% |
| ! | TGTTGAAATAAACTGAAACT+AGG | - | chr2.1:11387712-11387731 | MS.gene055860:CDS | 25.0% |
| ! | TTAAAGTTATTAGAAGCTTG+GGG | - | chr2.1:11388123-11388142 | MS.gene055860:CDS | 25.0% |
| ! | TTCAAGAGTAAAAAATCTGT+TGG | - | chr2.1:11387458-11387477 | MS.gene055860:CDS | 25.0% |
| !!! | ATTTTTGAAATTGTAACGGT+CGG | + | chr2.1:11388333-11388352 | None:intergenic | 25.0% |
| !!! | CATAACATTATTTCTGAACT+TGG | + | chr2.1:11387944-11387963 | None:intergenic | 25.0% |
| !!! | TTTTTTTATGCTTAACTTCC+AGG | - | chr2.1:11388452-11388471 | MS.gene055860:CDS | 25.0% |
| AAATTCAAGCATAACTAGCT+CGG | - | chr2.1:11387139-11387158 | MS.gene055860:CDS | 30.0% | |
| AATGATCAAACAAGAGCAAT+TGG | - | chr2.1:11386990-11387009 | MS.gene055860:intron | 30.0% | |
| ACATAACATATCTGTTGTTG+AGG | - | chr2.1:11388812-11388831 | MS.gene055860:CDS | 30.0% | |
| ATTGTCTAAGTGATCATGAT+AGG | - | chr2.1:11388410-11388429 | MS.gene055860:CDS | 30.0% | |
| CTGTAAATTGTATATTCACC+TGG | + | chr2.1:11388473-11388492 | None:intergenic | 30.0% | |
| GCTAAACATTGTGGTTAATT+AGG | - | chr2.1:11388588-11388607 | MS.gene055860:CDS | 30.0% | |
| GTTTATTTCAACAAGTGTTC+CGG | + | chr2.1:11387705-11387724 | None:intergenic | 30.0% | |
| TCTAACATTGGGAATTTGAA+CGG | - | chr2.1:11387281-11387300 | MS.gene055860:intron | 30.0% | |
| TTCAATTTGAAGATTGGCAA+AGG | - | chr2.1:11388969-11388988 | MS.gene055860:CDS | 30.0% | |
| TTGTCTAAGTGATCATGATA+GGG | - | chr2.1:11388411-11388430 | MS.gene055860:CDS | 30.0% | |
| ! | TAGTTTAGCATTTTCATCCA+AGG | + | chr2.1:11387111-11387130 | None:intergenic | 30.0% |
| ! | TTGATAATCGTTTGATTCAG+TGG | - | chr2.1:11387540-11387559 | MS.gene055860:intron | 30.0% |
| !! | AGTAGTTTTTCAACTCCTTT+CGG | + | chr2.1:11387419-11387438 | None:intergenic | 30.0% |
| !! | AGTTGTTTTCATAAGTTGTC+CGG | - | chr2.1:11387683-11387702 | MS.gene055860:CDS | 30.0% |
| !! | GGAAGTTAACATTGACTTAA+TGG | + | chr2.1:11388532-11388551 | None:intergenic | 30.0% |
| AAGAGGACTAATTCCATTAC+GGG | - | chr2.1:11387326-11387345 | MS.gene055860:intron | 35.0% | |
| ACAAGAGTTCGAAATCACAA+TGG | - | chr2.1:11387351-11387370 | MS.gene055860:intron | 35.0% | |
| AGAGCAATTTGATATGTGTC+CGG | - | chr2.1:11387908-11387927 | MS.gene055860:intron | 35.0% | |
| CAAAGCTTCATTGTTGGATT+CGG | + | chr2.1:11387171-11387190 | None:intergenic | 35.0% | |
| CAAGAGTTCGAAATCACAAT+GGG | - | chr2.1:11387352-11387371 | MS.gene055860:intron | 35.0% | |
| GAAGAGGACTAATTCCATTA+CGG | - | chr2.1:11387325-11387344 | MS.gene055860:intron | 35.0% | |
| GAATTAGTCCTCTTCATCAT+CGG | + | chr2.1:11387320-11387339 | None:intergenic | 35.0% | |
| GAGCTTCTGATTCTAACATT+GGG | - | chr2.1:11387270-11387289 | MS.gene055860:intron | 35.0% | |
| GGAAAAATTTCTCTGTCAAC+AGG | - | chr2.1:11388704-11388723 | MS.gene055860:CDS | 35.0% | |
| GTTAATGACGCTAAACATTG+TGG | - | chr2.1:11388579-11388598 | MS.gene055860:CDS | 35.0% | |
| TCTTCTGCGAAAAATAAGTC+TGG | - | chr2.1:11386840-11386859 | MS.gene055860:intron | 35.0% | |
| TGCTGCAATACTATCAGAAT+TGG | - | chr2.1:11388737-11388756 | MS.gene055860:CDS | 35.0% | |
| TTAGACAAACAAGTGTACCT+CGG | + | chr2.1:11387618-11387637 | None:intergenic | 35.0% | |
| TTGTTTCCAAAACCATTCGA+CGG | + | chr2.1:11387494-11387513 | None:intergenic | 35.0% | |
| ! | AAATTCAGTGGATTCTATGG+AGG | + | chr2.1:11386712-11386731 | None:intergenic | 35.0% |
| ! | AGGAAATTCAGTGGATTCTA+TGG | + | chr2.1:11386715-11386734 | None:intergenic | 35.0% |
| !! | ACAACTTTGAAACTTACTGC+AGG | + | chr2.1:11388561-11388580 | None:intergenic | 35.0% |
| !! | ATTGCTATTTCGCAAACTTG+AGG | - | chr2.1:11388296-11388315 | MS.gene055860:CDS | 35.0% |
| !! | GACTTATTTTTCGCAGAAGA+AGG | + | chr2.1:11386840-11386859 | None:intergenic | 35.0% |
| !! | TTGCTATTTCGCAAACTTGA+GGG | - | chr2.1:11388297-11388316 | MS.gene055860:CDS | 35.0% |
| AACTGCAGGATTTACTGCTA+GGG | - | chr2.1:11388999-11389018 | MS.gene055860:CDS | 40.0% | |
| AATTTGATATGTGTCCGGGA+TGG | - | chr2.1:11387913-11387932 | MS.gene055860:intron | 40.0% | |
| ACCTTAGAACCTTCCAAAAG+AGG | + | chr2.1:11388222-11388241 | None:intergenic | 40.0% | |
| AGAAACCTTCTTTGTTGACG+AGG | + | chr2.1:11386675-11386694 | None:intergenic | 40.0% | |
| AGAAGCCAAAGCTTCATTGT+TGG | + | chr2.1:11387177-11387196 | None:intergenic | 40.0% | |
| AGAATGCAAACACTCTTTGG+TGG | + | chr2.1:11388184-11388203 | None:intergenic | 40.0% | |
| AGAGGACTAATTCCATTACG+GGG | - | chr2.1:11387327-11387346 | MS.gene055860:intron | 40.0% | |
| AGCAGAATGCAAACACTCTT+TGG | + | chr2.1:11388187-11388206 | None:intergenic | 40.0% | |
| CGAATCCAACAATGAAGCTT+TGG | - | chr2.1:11387169-11387188 | MS.gene055860:CDS | 40.0% | |
| CTAAGTGATCATGATAGGGA+TGG | - | chr2.1:11388415-11388434 | MS.gene055860:CDS | 40.0% | |
| GAAACCTTCTTTGTTGACGA+GGG | + | chr2.1:11386674-11386693 | None:intergenic | 40.0% | |
| GAAACTTACTGCAGGTGAAA+AGG | + | chr2.1:11388553-11388572 | None:intergenic | 40.0% | |
| GAGCAATTTGATATGTGTCC+GGG | - | chr2.1:11387909-11387928 | MS.gene055860:intron | 40.0% | |
| GATCAAACAAGAGCAATTGG+TGG | - | chr2.1:11386993-11387012 | MS.gene055860:intron | 40.0% | |
| GCAAAGGAAATATCAACTGC+AGG | - | chr2.1:11388985-11389004 | MS.gene055860:CDS | 40.0% | |
| GCCAATCTTCAAATTGAACC+AGG | + | chr2.1:11388967-11388986 | None:intergenic | 40.0% | |
| GCCTGGTTCAATTTGAAGAT+TGG | - | chr2.1:11388963-11388982 | MS.gene055860:CDS | 40.0% | |
| GGAGCTTCTGATTCTAACAT+TGG | - | chr2.1:11387269-11387288 | MS.gene055860:intron | 40.0% | |
| ! | AATCGATGACTTGCGTATGA+CGG | + | chr2.1:11386748-11386767 | None:intergenic | 40.0% |
| ! | TCATACGCAAGTCATCGATT+CGG | - | chr2.1:11386748-11386767 | MS.gene055860:intron | 40.0% |
| !! | TCCTCTTTTGGAAGGTTCTA+AGG | - | chr2.1:11388218-11388237 | MS.gene055860:CDS | 40.0% |
| AACCATTCGACGGCGAATTA+AGG | + | chr2.1:11387484-11387503 | None:intergenic | 45.0% | |
| AATGGAAGCTGTGAGATCAG+AGG | + | chr2.1:11388514-11388533 | None:intergenic | 45.0% | |
| ACATTGAGAAGGGCTAGAAG+AGG | - | chr2.1:11387248-11387267 | MS.gene055860:intron | 45.0% | |
| ATTAGAAGCTTGGGGAAGCA+TGG | - | chr2.1:11388131-11388150 | MS.gene055860:CDS | 45.0% | |
| CAACTGCAGGATTTACTGCT+AGG | - | chr2.1:11388998-11389017 | MS.gene055860:CDS | 45.0% | |
| CGTTGAAACCGATGATGAAG+AGG | - | chr2.1:11387309-11387328 | MS.gene055860:intron | 45.0% | |
| GAGTGACGCAACATTGAGAA+GGG | - | chr2.1:11387238-11387257 | MS.gene055860:intron | 45.0% | |
| TCATCCAAGGAAAACCTTCC+TGG | + | chr2.1:11387098-11387117 | None:intergenic | 45.0% | |
| TGAACCCTCGTCAACAAAGA+AGG | - | chr2.1:11386667-11386686 | MS.gene055860:CDS | 45.0% | |
| TGAGTGACGCAACATTGAGA+AGG | - | chr2.1:11387237-11387256 | MS.gene055860:intron | 45.0% | |
| ! | AATGGTTCAGGCATTGTTCC+AGG | - | chr2.1:11387077-11387096 | MS.gene055860:intron | 45.0% |
| ! | AATTCGCCGTCGAATGGTTT+TGG | - | chr2.1:11387485-11387504 | MS.gene055860:CDS | 45.0% |
| ! | ACAAGAGCAATTGGTGGTTC+TGG | - | chr2.1:11386999-11387018 | MS.gene055860:intron | 45.0% |
| ! | CAATGAAGCTTTGGCTTCTC+CGG | - | chr2.1:11387178-11387197 | MS.gene055860:CDS | 45.0% |
| ! | TACAGCCATTGCTATTCGTC+AGG | - | chr2.1:11388488-11388507 | MS.gene055860:CDS | 45.0% |
| ! | TGCAGAGTTCCTCTTTTGGA+AGG | - | chr2.1:11388210-11388229 | MS.gene055860:CDS | 45.0% |
| ! | TGTGTGCAGAGTTCCTCTTT+TGG | - | chr2.1:11388206-11388225 | MS.gene055860:CDS | 45.0% |
| ! | TGTTCCAGGAAGGTTTTCCT+TGG | - | chr2.1:11387091-11387110 | MS.gene055860:intron | 45.0% |
| ! | TTCAGTGGATTCTATGGAGG+TGG | + | chr2.1:11386709-11386728 | None:intergenic | 45.0% |
| ! | TTCTGAGGGCTGCATTCATA+TGG | - | chr2.1:11388683-11388702 | MS.gene055860:CDS | 45.0% |
| AAGCAGTGTAAAGTGCAGCC+TGG | - | chr2.1:11388946-11388965 | MS.gene055860:CDS | 50.0% | |
| AGAGGCCTGACGAATAGCAA+TGG | + | chr2.1:11388496-11388515 | None:intergenic | 50.0% | |
| CGAACTCTTGTACCCCGTAA+TGG | + | chr2.1:11387342-11387361 | None:intergenic | 50.0% | |
| CTGTTCTACGTCGTCAACTC+TGG | - | chr2.1:11386814-11386833 | MS.gene055860:intron | 50.0% | |
| CTTGAAAACTCTGACCTCCC+TGG | + | chr2.1:11387386-11387405 | None:intergenic | 50.0% | |
| CTTGTAGGAACTTCCACCAC+TGG | + | chr2.1:11387212-11387231 | None:intergenic | 50.0% | |
| CTTTGTTGACGAGGGTTCAC+CGG | + | chr2.1:11386666-11386685 | None:intergenic | 50.0% | |
| GCGTCACTCATCAACCTTGT+AGG | + | chr2.1:11387227-11387246 | None:intergenic | 50.0% | |
| GGAAGGGGAAGGAAATTCAG+TGG | + | chr2.1:11386724-11386743 | None:intergenic | 50.0% | |
| GTGGATTCTATGGAGGTGGA+AGG | + | chr2.1:11386705-11386724 | None:intergenic | 50.0% | |
| TCAACTCCTTTCGGCTTCGT+GGG | + | chr2.1:11387410-11387429 | None:intergenic | 50.0% | |
| TGTGTCTCCTATCGTCTTCC+TGG | + | chr2.1:11386776-11386795 | None:intergenic | 50.0% | |
| TTCAACTCCTTTCGGCTTCG+TGG | + | chr2.1:11387411-11387430 | None:intergenic | 50.0% | |
| TTCTCCGGCAAGAAAACCAG+TGG | - | chr2.1:11387193-11387212 | MS.gene055860:intron | 50.0% | |
| ! | AAGCTTGGGGAAGCATGGAA+AGG | - | chr2.1:11388136-11388155 | MS.gene055860:CDS | 50.0% |
| ! | ACTTGCGTATGACGGAGGAA+GGG | + | chr2.1:11386740-11386759 | None:intergenic | 50.0% |
| ! | CAGTGGTGGAAGTTCCTACA+AGG | - | chr2.1:11387210-11387229 | MS.gene055860:intron | 50.0% |
| ! | GATAGGGATGGTGCATTGAC+AGG | - | chr2.1:11388427-11388446 | MS.gene055860:CDS | 50.0% |
| ! | GTTCAGGCATTGTTCCAGGA+AGG | - | chr2.1:11387081-11387100 | MS.gene055860:intron | 50.0% |
| ! | TGAACTTGGTGAGACCATCC+CGG | + | chr2.1:11387930-11387949 | None:intergenic | 50.0% |
| !! | GGTGGTTCTGGAAGATTCAC+AGG | - | chr2.1:11387011-11387030 | MS.gene055860:intron | 50.0% |
| !!! | TCCACCACTGGTTTTCTTGC+CGG | + | chr2.1:11387200-11387219 | None:intergenic | 50.0% |
| AAACATTTCTCGCCACGCCG+AGG | - | chr2.1:11387598-11387617 | MS.gene055860:intron | 55.0% | |
| CAAACAAGTGTACCTCGGCG+TGG | + | chr2.1:11387613-11387632 | None:intergenic | 55.0% | |
| CAACTCCTTTCGGCTTCGTG+GGG | + | chr2.1:11387409-11387428 | None:intergenic | 55.0% | |
| GGCCTTAATTCGCCGTCGAA+TGG | - | chr2.1:11387479-11387498 | MS.gene055860:CDS | 55.0% | |
| TCCGGCAAGAAAACCAGTGG+TGG | - | chr2.1:11387196-11387215 | MS.gene055860:intron | 55.0% | |
| TCTATGGAGGTGGAAGGCGA+GGG | + | chr2.1:11386699-11386718 | None:intergenic | 55.0% | |
| TTCGGAGCCAGGAAGACGAT+AGG | - | chr2.1:11386766-11386785 | MS.gene055860:intron | 55.0% | |
| TTCTATGGAGGTGGAAGGCG+AGG | + | chr2.1:11386700-11386719 | None:intergenic | 55.0% | |
| ! | CACAATGGGCTTTGTCGCCA+GGG | - | chr2.1:11387366-11387385 | MS.gene055860:intron | 55.0% |
| ! | CGATGACTTGCGTATGACGG+AGG | + | chr2.1:11386745-11386764 | None:intergenic | 55.0% |
| ! | CTTGCGTATGACGGAGGAAG+GGG | + | chr2.1:11386739-11386758 | None:intergenic | 55.0% |
| ! | GAAGGCGCGAAACACTTCTC+CGG | - | chr2.1:11386644-11386663 | MS.gene055860:CDS | 55.0% |
| ! | GACTTGCGTATGACGGAGGA+AGG | + | chr2.1:11386741-11386760 | None:intergenic | 55.0% |
| ! | TCACAATGGGCTTTGTCGCC+AGG | - | chr2.1:11387365-11387384 | MS.gene055860:intron | 55.0% |
| !! | CAAGTCATCGATTCGGAGCC+AGG | - | chr2.1:11386755-11386774 | MS.gene055860:intron | 55.0% |
| TCAAGCCCCACGAAGCCGAA+AGG | - | chr2.1:11387401-11387420 | MS.gene055860:intron | 60.0% | |
| ! | AATGGGCTTTGTCGCCAGGG+AGG | - | chr2.1:11387369-11387388 | MS.gene055860:intron | 60.0% |
| ! | CGTATGACGGAGGAAGGGGA+AGG | + | chr2.1:11386735-11386754 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 11386642 | 11389033 | 11386642 | ID=MS.gene055860 |
| chr2.1 | mRNA | 11386642 | 11389033 | 11386642 | ID=MS.gene055860.t1;Parent=MS.gene055860 |
| chr2.1 | exon | 11388056 | 11389033 | 11388056 | ID=MS.gene055860.t1.exon1;Parent=MS.gene055860.t1 |
| chr2.1 | CDS | 11388056 | 11389033 | 11388056 | ID=cds.MS.gene055860.t1;Parent=MS.gene055860.t1 |
| chr2.1 | exon | 11387646 | 11387765 | 11387646 | ID=MS.gene055860.t1.exon2;Parent=MS.gene055860.t1 |
| chr2.1 | CDS | 11387646 | 11387765 | 11387646 | ID=cds.MS.gene055860.t1;Parent=MS.gene055860.t1 |
| chr2.1 | exon | 11387436 | 11387552 | 11387436 | ID=MS.gene055860.t1.exon3;Parent=MS.gene055860.t1 |
| chr2.1 | CDS | 11387436 | 11387552 | 11387436 | ID=cds.MS.gene055860.t1;Parent=MS.gene055860.t1 |
| chr2.1 | exon | 11387112 | 11387201 | 11387112 | ID=MS.gene055860.t1.exon4;Parent=MS.gene055860.t1 |
| chr2.1 | CDS | 11387112 | 11387201 | 11387112 | ID=cds.MS.gene055860.t1;Parent=MS.gene055860.t1 |
| chr2.1 | exon | 11386842 | 11386949 | 11386842 | ID=MS.gene055860.t1.exon5;Parent=MS.gene055860.t1 |
| chr2.1 | CDS | 11386842 | 11386949 | 11386842 | ID=cds.MS.gene055860.t1;Parent=MS.gene055860.t1 |
| chr2.1 | exon | 11386642 | 11386737 | 11386642 | ID=MS.gene055860.t1.exon6;Parent=MS.gene055860.t1 |
| chr2.1 | CDS | 11386642 | 11386737 | 11386642 | ID=cds.MS.gene055860.t1;Parent=MS.gene055860.t1 |
| Gene Sequence |
| Protein sequence |
