Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72583.t1 | KEH29461.1 | 57 | 86 | 27 | 1 | 1 | 76 | 18 | 103 | 3.60E-15 | 90.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72583.t1 | A0A072UJ67 | 57.0 | 86 | 27 | 1 | 1 | 76 | 18 | 103 | 2.6e-15 | 90.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050414 | MS.gene72583 | 0.802375 | 5.78E-49 | -1.69E-46 |
| MS.gene056677 | MS.gene72583 | 0.833857 | 4.19E-56 | -1.69E-46 |
| MS.gene059088 | MS.gene72583 | 0.811681 | 6.20E-51 | -1.69E-46 |
| MS.gene059170 | MS.gene72583 | 0.805485 | 1.31E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene72583 | 0.808948 | 2.41E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72583.t1 | MTR_4g037005 | 69.767 | 86 | 16 | 1 | 1 | 76 | 18 | 103 | 7.59e-39 | 124 |
| MS.gene72583.t1 | MTR_7g028435 | 67.045 | 88 | 17 | 2 | 1 | 76 | 18 | 105 | 2.55e-36 | 118 |
| MS.gene72583.t1 | MTR_4g037005 | 67.045 | 88 | 17 | 2 | 1 | 76 | 18 | 105 | 2.55e-36 | 118 |
| MS.gene72583.t1 | MTR_4g036795 | 69.697 | 66 | 13 | 1 | 1 | 66 | 18 | 76 | 1.89e-27 | 94.7 |
| MS.gene72583.t1 | MTR_4g036990 | 50.000 | 76 | 16 | 3 | 1 | 76 | 18 | 71 | 7.46e-15 | 62.8 |
| MS.gene72583.t1 | MTR_8g070055 | 43.750 | 80 | 28 | 3 | 1 | 76 | 18 | 84 | 1.28e-14 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTCTTACATCGCTGATC+TGG | 0.242771 | 4.1:-61550791 | None:intergenic |
| GCACTTAATAAAACTGCTAT+TGG | 0.316112 | 4.1:+61550848 | MS.gene72583:CDS |
| CTCTCTTACATCGCTGATCT+GGG | 0.334871 | 4.1:-61550790 | None:intergenic |
| AAAGCAACAAGGAACATTCT+TGG | 0.424631 | 4.1:+61550635 | MS.gene72583:CDS |
| CACTTACTTGCATACATGTC+AGG | 0.457216 | 4.1:-61550662 | None:intergenic |
| TTTATGGTTTCAGATGAAAT+GGG | 0.525004 | 4.1:+61550746 | MS.gene72583:intron |
| CCAGATCAGCGATGTAAGAG+AGG | 0.582999 | 4.1:+61550791 | MS.gene72583:CDS |
| CAAAGATAATTGTGGCAACG+TGG | 0.636077 | 4.1:-61550908 | None:intergenic |
| TCTCTTACATCGCTGATCTG+GGG | 0.649695 | 4.1:-61550789 | None:intergenic |
| GGCCAGATCAAAGATAATTG+TGG | 0.654891 | 4.1:-61550916 | None:intergenic |
| GGACTTAATCAAACACACGA+GGG | 0.688157 | 4.1:+61550869 | MS.gene72583:CDS |
| TGGACTTAATCAAACACACG+AGG | 0.700580 | 4.1:+61550868 | MS.gene72583:CDS |
| GTTTCTCTACTAAAGCAACA+AGG | 0.710702 | 4.1:+61550624 | MS.gene72583:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAAGAACAAATTATATA+TGG | - | chr4.1:61550731-61550750 | None:intergenic | 10.0% |
| !!! | ATATATAATTTGTTCTTTTA+TGG | + | chr4.1:61550730-61550749 | MS.gene72583:intron | 10.0% |
| ! | TTTATGGTTTCAGATGAAAT+GGG | + | chr4.1:61550746-61550765 | MS.gene72583:intron | 25.0% |
| !! | TTTTATGGTTTCAGATGAAA+TGG | + | chr4.1:61550745-61550764 | MS.gene72583:intron | 25.0% |
| GCACTTAATAAAACTGCTAT+TGG | + | chr4.1:61550848-61550867 | MS.gene72583:CDS | 30.0% | |
| !!! | AGCAGTTTTATTAAGTGCAA+CGG | - | chr4.1:61550846-61550865 | None:intergenic | 30.0% |
| AAAGCAACAAGGAACATTCT+TGG | + | chr4.1:61550635-61550654 | MS.gene72583:CDS | 35.0% | |
| GTTTCTCTACTAAAGCAACA+AGG | + | chr4.1:61550624-61550643 | MS.gene72583:CDS | 35.0% | |
| !!! | GCAGTTTTATTAAGTGCAAC+GGG | - | chr4.1:61550845-61550864 | None:intergenic | 35.0% |
| CAAAGATAATTGTGGCAACG+TGG | - | chr4.1:61550911-61550930 | None:intergenic | 40.0% | |
| CACTTACTTGCATACATGTC+AGG | - | chr4.1:61550665-61550684 | None:intergenic | 40.0% | |
| GGACTTAATCAAACACACGA+GGG | + | chr4.1:61550869-61550888 | MS.gene72583:CDS | 40.0% | |
| TGGACTTAATCAAACACACG+AGG | + | chr4.1:61550868-61550887 | MS.gene72583:CDS | 40.0% | |
| ACGCAAGCACACAAATGGAA+TGG | - | chr4.1:61550705-61550724 | None:intergenic | 45.0% | |
| CGCAAGCACACAAATGGAAT+GGG | - | chr4.1:61550704-61550723 | None:intergenic | 45.0% | |
| CTCTCTTACATCGCTGATCT+GGG | - | chr4.1:61550793-61550812 | None:intergenic | 45.0% | |
| GGAAAACGCAAGCACACAAA+TGG | - | chr4.1:61550710-61550729 | None:intergenic | 45.0% | |
| TCTCTTACATCGCTGATCTG+GGG | - | chr4.1:61550792-61550811 | None:intergenic | 45.0% | |
| CCAGATCAGCGATGTAAGAG+AGG | + | chr4.1:61550791-61550810 | MS.gene72583:CDS | 50.0% | |
| CCTCTCTTACATCGCTGATC+TGG | - | chr4.1:61550794-61550813 | None:intergenic | 50.0% | |
| ! | TGGCAACGTGGATCGTTTTC+TGG | - | chr4.1:61550899-61550918 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 61550620 | 61550931 | 61550620 | ID=MS.gene72583 |
| chr4.1 | mRNA | 61550620 | 61550931 | 61550620 | ID=MS.gene72583.t1;Parent=MS.gene72583 |
| chr4.1 | exon | 61550620 | 61550677 | 61550620 | ID=MS.gene72583.t1.exon1;Parent=MS.gene72583.t1 |
| chr4.1 | CDS | 61550620 | 61550677 | 61550620 | ID=cds.MS.gene72583.t1;Parent=MS.gene72583.t1 |
| chr4.1 | exon | 61550759 | 61550931 | 61550759 | ID=MS.gene72583.t1.exon2;Parent=MS.gene72583.t1 |
| chr4.1 | CDS | 61550759 | 61550931 | 61550759 | ID=cds.MS.gene72583.t1;Parent=MS.gene72583.t1 |
| Gene Sequence |
| Protein sequence |
