AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72989

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72989.t1 MTR_4g102790 98.527 679 7 2 1 678 1 677 0.0 1378
MS.gene72989.t1 MTR_7g027190 37.722 395 223 7 281 662 288 672 9.12e-81 270
MS.gene72989.t1 MTR_7g074650 36.856 369 215 10 297 664 442 793 3.57e-63 224
MS.gene72989.t1 MTR_1g069725 33.810 420 247 11 256 663 63 463 1.14e-58 205
MS.gene72989.t1 MTR_5g094450 33.418 395 227 10 286 662 155 531 5.18e-58 205
MS.gene72989.t1 MTR_4g104020 34.367 387 233 13 287 662 143 519 1.05e-56 201
MS.gene72989.t1 MTR_8g442410 29.259 499 317 12 185 662 41 524 3.36e-56 200
MS.gene72989.t1 MTR_3g065980 31.328 399 236 8 282 662 161 539 1.40e-55 199
MS.gene72989.t1 MTR_3g089055 36.126 382 217 14 293 662 203 569 1.76e-55 199
MS.gene72989.t1 MTR_3g089055 36.126 382 217 14 293 662 198 564 1.83e-55 199
MS.gene72989.t1 MTR_1g096030 33.088 408 226 14 283 662 70 458 1.05e-54 194
MS.gene72989.t1 MTR_1g029420 35.079 382 221 13 293 662 225 591 4.70e-54 195
MS.gene72989.t1 MTR_4g076140 28.689 488 266 11 236 662 2 468 5.47e-54 192
MS.gene72989.t1 MTR_5g097480 33.508 382 233 11 290 662 174 543 4.16e-53 192
MS.gene72989.t1 MTR_2g082090 32.282 412 244 13 269 662 184 578 4.96e-53 192
MS.gene72989.t1 MTR_3g056110 30.769 403 249 9 273 662 156 541 8.38e-51 185
MS.gene72989.t1 MTR_3g022830 26.777 422 244 7 294 662 26 435 4.17e-47 172
MS.gene72989.t1 MTR_4g076020 33.245 376 216 14 297 667 88 433 5.24e-47 172
MS.gene72989.t1 MTR_2g097410 28.979 421 274 11 258 664 333 742 5.31e-46 175
MS.gene72989.t1 MTR_4g133660 29.610 385 249 9 288 662 170 542 4.65e-45 169
MS.gene72989.t1 MTR_2g097310 26.302 384 263 8 289 664 268 639 6.47e-45 170
MS.gene72989.t1 MTR_2g026250 28.319 452 299 12 221 662 159 595 1.46e-44 169
MS.gene72989.t1 MTR_2g097380 27.763 371 223 9 300 664 231 562 6.80e-44 166
MS.gene72989.t1 MTR_4g097080 27.249 389 251 9 292 664 132 504 7.62e-44 165
MS.gene72989.t1 MTR_4g077760 31.152 382 237 12 290 662 190 554 9.10e-44 166
MS.gene72989.t1 MTR_2g097390 26.684 386 259 9 289 664 271 642 2.44e-43 166
MS.gene72989.t1 MTR_4g064160 27.040 429 288 10 250 664 268 685 1.58e-41 161
MS.gene72989.t1 MTR_4g064120 27.404 416 274 11 266 664 223 627 5.85e-41 159
MS.gene72989.t1 MTR_4g064150 27.273 407 268 12 274 665 342 735 1.82e-40 158
MS.gene72989.t1 MTR_2g097463 24.031 516 326 12 197 664 159 656 1.89e-40 157
MS.gene72989.t1 MTR_5g009080 32.443 262 166 2 239 500 9 259 2.57e-40 154
MS.gene72989.t1 MTR_5g009080 33.333 153 95 5 511 662 331 477 6.91e-19 90.5
MS.gene72989.t1 MTR_5g058860 31.785 409 239 16 279 663 105 497 2.94e-40 155
MS.gene72989.t1 MTR_3g072710 31.127 408 237 16 283 663 110 500 3.33e-40 154
MS.gene72989.t1 MTR_2g097350 25.515 388 261 7 289 664 270 641 4.02e-40 156
MS.gene72989.t1 MTR_4g064180 27.525 396 263 10 282 664 243 627 4.45e-40 156
MS.gene72989.t1 MTR_5g015490 28.608 388 253 11 292 664 113 491 7.47e-40 153
MS.gene72989.t1 MTR_4g064200 26.494 385 261 9 289 664 280 651 1.15e-39 155
MS.gene72989.t1 MTR_2g034250 28.109 402 268 10 276 665 195 587 1.44e-39 154
MS.gene72989.t1 MTR_2g034280 27.820 399 264 12 276 662 188 574 1.76e-37 148
MS.gene72989.t1 MTR_2g034260 27.930 401 263 11 276 662 195 583 1.88e-37 148
MS.gene72989.t1 MTR_2g097467 24.936 389 265 9 287 664 283 655 3.27e-37 148
MS.gene72989.t1 MTR_7g062120 24.936 389 264 8 289 664 368 741 1.15e-36 147
MS.gene72989.t1 MTR_8g093070 29.383 405 241 16 278 666 124 499 2.70e-36 143
MS.gene72989.t1 MTR_7g109580 26.992 389 263 10 282 662 178 553 5.39e-36 143
MS.gene72989.t1 MTR_2g089100 25.616 406 261 10 293 667 52 447 1.37e-35 140
MS.gene72989.t1 MTR_2g097473 24.524 420 279 11 267 664 252 655 5.66e-35 141
MS.gene72989.t1 MTR_1g086970 24.359 390 279 10 282 662 95 477 3.18e-34 137
MS.gene72989.t1 MTR_2g099110 25.679 405 242 10 293 664 74 452 5.14e-32 130
MS.gene72989.t1 MTR_7g057230 34.221 263 160 10 405 662 402 656 2.40e-31 130
MS.gene72989.t1 MTR_5g015950 24.048 420 264 13 293 664 35 447 1.64e-25 110
MS.gene72989.t1 MTR_3g053270 24.048 420 264 13 293 664 35 447 1.64e-25 110
MS.gene72989.t1 MTR_6g047750 32.222 270 162 10 405 662 401 661 1.96e-25 112
MS.gene72989.t1 MTR_4g026485 25.526 380 246 11 294 662 270 623 1.09e-24 109
MS.gene72989.t1 MTR_0092s0100 24.814 403 277 9 266 662 345 727 2.69e-23 105
MS.gene72989.t1 MTR_0092s0100 24.378 402 280 7 266 662 348 730 3.04e-23 105
MS.gene72989.t1 MTR_4g095500 22.673 419 269 13 293 664 59 469 4.70e-19 91.3
MS.gene72989.t1 MTR_8g077940 27.577 359 218 14 319 662 209 540 3.99e-17 85.5
MS.gene72989.t1 MTR_8g020840 23.786 412 237 15 293 662 171 547 1.37e-11 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72989.t1 AT1G63100 55.777 701 225 23 1 663 1 654 0.0 689
MS.gene72989.t1 AT1G63100 53.638 701 215 21 1 663 1 629 0.0 655
MS.gene72989.t1 AT1G66350 34.961 389 218 11 282 662 144 505 5.94e-67 229
MS.gene72989.t1 AT2G01570 35.990 389 220 11 282 662 213 580 2.01e-65 226
MS.gene72989.t1 AT1G50420 32.360 445 231 10 279 662 43 478 6.14e-65 223
MS.gene72989.t1 AT1G14920 35.219 389 223 11 282 662 161 528 1.12e-64 223
MS.gene72989.t1 AT3G54220 34.949 392 238 8 273 664 277 651 2.40e-64 225
MS.gene72989.t1 AT3G03450 33.162 389 236 11 282 662 172 544 1.62e-61 215
MS.gene72989.t1 AT5G17490 33.073 384 233 11 284 662 151 515 9.41e-60 209
MS.gene72989.t1 AT5G41920 35.641 390 228 13 278 662 28 399 5.58e-59 204
MS.gene72989.t1 AT1G55580 35.036 411 222 12 290 662 41 444 3.61e-53 189
MS.gene72989.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.23e-48 178
MS.gene72989.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.23e-48 178
MS.gene72989.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.23e-48 178
MS.gene72989.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.23e-48 178
MS.gene72989.t1 AT5G66770 32.172 373 217 12 302 662 235 583 1.77e-48 180
MS.gene72989.t1 AT2G04890 32.540 378 237 12 289 662 49 412 4.52e-48 175
MS.gene72989.t1 AT1G50600 31.552 393 242 12 285 662 145 525 1.01e-47 176
MS.gene72989.t1 AT1G50600 31.980 394 239 13 285 662 216 596 3.44e-47 176
MS.gene72989.t1 AT1G50600 31.980 394 239 13 285 662 220 600 3.47e-47 176
MS.gene72989.t1 AT4G17230 32.041 387 240 10 288 664 153 526 1.80e-46 173
MS.gene72989.t1 AT4G17230 32.041 387 240 10 288 664 153 526 1.80e-46 173
MS.gene72989.t1 AT1G21450 30.900 411 256 11 268 662 194 592 1.37e-45 172
MS.gene72989.t1 AT2G29065 31.026 390 241 13 292 664 251 629 1.28e-42 164
MS.gene72989.t1 AT3G50650 32.609 368 209 14 313 662 195 541 1.81e-42 162
MS.gene72989.t1 AT2G29060 27.619 420 265 12 258 665 265 657 2.57e-41 160
MS.gene72989.t1 AT2G29060 27.619 420 265 12 258 665 302 694 3.27e-41 160
MS.gene72989.t1 AT1G07530 27.979 386 257 10 289 664 392 766 2.55e-40 158
MS.gene72989.t1 AT1G07520 28.288 403 254 16 282 664 143 530 6.89e-40 154
MS.gene72989.t1 AT1G07520 28.288 403 254 16 282 664 143 530 6.89e-40 154
MS.gene72989.t1 AT5G59450 25.887 479 310 14 220 666 136 601 1.60e-39 154
MS.gene72989.t1 AT1G07520 28.288 403 254 16 282 664 307 694 1.71e-39 155
MS.gene72989.t1 AT1G07520 28.288 403 254 16 282 664 308 695 1.88e-39 155
MS.gene72989.t1 AT4G37650 27.481 393 258 9 292 664 145 530 3.47e-39 152
MS.gene72989.t1 AT4G08250 28.856 402 250 14 279 662 93 476 5.12e-39 150
MS.gene72989.t1 AT3G46600 26.877 413 269 10 261 664 192 580 1.66e-36 145
MS.gene72989.t1 AT3G46600 28.010 382 254 9 292 664 3 372 2.28e-36 141
MS.gene72989.t1 AT3G46600 27.119 413 273 10 268 664 148 548 5.14e-36 143
MS.gene72989.t1 AT2G37650 25.700 393 254 11 289 664 331 702 2.94e-32 133
MS.gene72989.t1 AT2G37650 25.700 393 254 11 289 664 343 714 3.71e-32 133
MS.gene72989.t1 AT5G52510 29.283 321 193 10 367 662 328 639 2.94e-27 117
MS.gene72989.t1 AT3G49950 26.700 397 251 12 293 665 30 410 4.66e-26 111
MS.gene72989.t1 AT4G00150 28.011 357 222 12 312 662 226 553 3.67e-25 110
MS.gene72989.t1 AT3G60630 27.597 308 196 12 369 662 327 621 9.92e-24 106
MS.gene72989.t1 AT2G45160 27.302 315 185 11 369 662 347 638 1.84e-21 99.8
MS.gene72989.t1 AT4G36710 23.650 389 261 12 287 662 119 484 1.70e-18 89.7
MS.gene72989.t1 AT3G13840 24.939 413 250 18 275 662 132 509 3.97e-17 85.5

Find 175 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCATTTCATTGCAAAAT+TGG 0.138280 8.3:+19017716 MS.gene72989:CDS
CCTTTCTCTTTGAATTGTTC+TGG 0.202054 8.3:+19017318 MS.gene72989:CDS
ACTGGTTGTGTTGATGCTAT+TGG 0.221753 8.3:+19017672 MS.gene72989:CDS
GTAACAAAGATCACCAATTT+TGG 0.231391 8.3:+19017426 MS.gene72989:CDS
AGATCAAGGATGAGGAGTTT+TGG 0.247813 8.3:+19017085 MS.gene72989:CDS
AGTTTCAGTTTGGCACAATT+TGG 0.253074 8.3:+19017234 MS.gene72989:CDS
TTGGAGAGGCACCAGAGTTT+TGG 0.253860 8.3:+19018557 MS.gene72989:CDS
CTCTCTACACAGTATCTTCT+TGG 0.270369 8.3:+19018753 MS.gene72989:CDS
TGTCACCAACTCACAGATTA+AGG 0.272810 8.3:+19016821 MS.gene72989:CDS
AACCATTTCATTGCAAAATT+GGG 0.272931 8.3:+19017717 MS.gene72989:CDS
CTGCTGTTGTTCTTGACTTC+AGG 0.279004 8.3:-19016994 None:intergenic
CTGGTTGTGTTGATGCTATT+GGG 0.293519 8.3:+19017673 MS.gene72989:CDS
AAATAGAGCTAAGAGGAAAA+AGG 0.307901 8.3:+19017170 MS.gene72989:CDS
ATAAGCACAAATTCGGCTTA+TGG 0.317498 8.3:-19017766 None:intergenic
GCTTGCAGAACATGGATCTT+TGG 0.333074 8.3:+19017134 MS.gene72989:CDS
CAATCCTACAATTGTTGTTA+TGG 0.340164 8.3:+19018343 MS.gene72989:CDS
TGGACACCGGTTGACGTTGC+TGG 0.348429 8.3:+19018773 MS.gene72989:CDS
CATGAAGAGGGTAATGGTTC+GGG 0.351755 8.3:+19017558 MS.gene72989:CDS
TGCCTTTGTCTAACAATCCT+TGG 0.358700 8.3:+19017391 MS.gene72989:CDS
AATACATCTTCAACAGAAAC+TGG 0.362646 8.3:-19018655 None:intergenic
TCAGTTCCTTCATGCTGATA+AGG 0.365523 8.3:-19017585 None:intergenic
CGATGTGAAATGAAGAAACT+TGG 0.373450 8.3:-19017934 None:intergenic
TCTGCCATAACAACAATTGT+AGG 0.376086 8.3:-19018347 None:intergenic
GTGGCTACCCTCACCAAAAT+TGG 0.379531 8.3:-19017439 None:intergenic
AATTTGGAGCTGGAAACTTT+TGG 0.382164 8.3:+19017250 MS.gene72989:CDS
TGAAAGGGAAAGTTTCAGTT+TGG 0.386013 8.3:+19017224 MS.gene72989:CDS
AATCTCTGTGTGCTCATTGA+TGG 0.390998 8.3:-19016886 None:intergenic
CGGTAAAATAAGCACAAATT+CGG 0.394214 8.3:-19017773 None:intergenic
GGAGCAACAGCACTTACTTT+GGG 0.396486 8.3:+19018716 MS.gene72989:CDS
GATGCATGGGAGTCACTGAT+AGG 0.410356 8.3:+19018618 MS.gene72989:CDS
ATCAATGAGCACACAGAGAT+TGG 0.410565 8.3:+19016888 MS.gene72989:CDS
GACTTGAAGATGTAAGGCTT+TGG 0.411643 8.3:+19018201 MS.gene72989:CDS
TGTTCCAAAGTTTAGCTTCA+AGG 0.419556 8.3:+19018045 MS.gene72989:CDS
CCATCAGAGTCACTAGGCTT+TGG 0.420891 8.3:+19017805 MS.gene72989:CDS
AGCAACAACTGCTCAGACAT+TGG 0.422074 8.3:+19017059 MS.gene72989:CDS
TCTTCAAGTCTGTCGACTAC+TGG 0.429762 8.3:-19018188 None:intergenic
TCGAACATGGCTTGGAGGAT+TGG 0.430304 8.3:-19018069 None:intergenic
ACATGAAGAGGGTAATGGTT+CGG 0.431411 8.3:+19017557 MS.gene72989:CDS
TTCGCAGCAGCCGTTGAGGC+CGG 0.436828 8.3:+19016951 MS.gene72989:CDS
AGGAGCAACAGCACTTACTT+TGG 0.437760 8.3:+19018715 MS.gene72989:CDS
AGAGTCAGAGTTTGAGCCTA+AGG 0.440522 8.3:+19017514 MS.gene72989:CDS
GACTCTGATGGCTAAGGCTT+CGG 0.442125 8.3:-19017793 None:intergenic
AAATGAAGAAACTTGGGAAT+TGG 0.443639 8.3:-19017927 None:intergenic
GCAACAACTGCTCAGACATT+GGG 0.444891 8.3:+19017060 MS.gene72989:CDS
AGATGTTAGCTAGGGAGATA+AGG 0.446179 8.3:+19018504 MS.gene72989:CDS
CAACCTGTCCCTTCCTTCGC+AGG 0.451825 8.3:-19018537 None:intergenic
AAGCAAGAGCTGAATGAAAC+CGG 0.462075 8.3:+19018113 MS.gene72989:CDS
TGGCCCTTGAAGCTAAACTT+TGG 0.467104 8.3:-19018049 None:intergenic
CCAGAACAATTCAAAGAGAA+AGG 0.469406 8.3:-19017318 None:intergenic
GAGCTTCCAGCAACGTCAAC+CGG 0.470552 8.3:-19018779 None:intergenic
GTTCTCTGCAGCAGAACATT+CGG 0.471882 8.3:+19017025 MS.gene72989:CDS
TTGTTCTGGAGATGAGGAAA+GGG 0.476638 8.3:+19017332 MS.gene72989:CDS
GAAACCGGAGACAGACTTGC+TGG 0.481646 8.3:+19018128 MS.gene72989:CDS
CATAACAACAATTGTAGGAT+TGG 0.483005 8.3:-19018342 None:intergenic
AGATAGAGGAGATGTTAGCT+AGG 0.487005 8.3:+19018495 MS.gene72989:CDS
CCAAGCCATGTTCGAATCAC+GGG 0.489720 8.3:+19018077 MS.gene72989:CDS
TGATCACAGTGGCCTTCCAC+TGG 0.491665 8.3:+19018454 MS.gene72989:CDS
TCAGACATTGGGAGAGATCA+AGG 0.493655 8.3:+19017071 MS.gene72989:CDS
CCATGTTCGAATCACGGGTG+TGG 0.493749 8.3:+19018082 MS.gene72989:CDS
TCCGATGAGGATCAAGATAG+AGG 0.495969 8.3:+19018481 MS.gene72989:CDS
TGCATTGAGGCTTCTAAACC+AGG 0.497162 8.3:+19017899 MS.gene72989:CDS
CACAGTATCTTCTTGGACAC+CGG 0.497435 8.3:+19018760 MS.gene72989:CDS
CATGTTCGAATCACGGGTGT+GGG 0.498098 8.3:+19018083 MS.gene72989:CDS
TCGACAGACTTGAAGATGTA+AGG 0.499051 8.3:+19018195 MS.gene72989:CDS
ATGCATTCTCATTCAACCTT+AGG 0.503609 8.3:-19017530 None:intergenic
CGGCTTATGGAAGTTCCCCT+TGG 0.503709 8.3:-19017753 None:intergenic
GTTCCAAAGTTTAGCTTCAA+GGG 0.504136 8.3:+19018046 MS.gene72989:CDS
GAAGGAACTGAAGTGGAGAC+AGG 0.505317 8.3:+19017597 MS.gene72989:CDS
ATCGAATGAGATGCTGTTGA+GGG 0.506115 8.3:+19017953 MS.gene72989:CDS
CCCGTGATTCGAACATGGCT+TGG 0.506282 8.3:-19018077 None:intergenic
TTGAAGAGGCTTGCAGAACA+TGG 0.509151 8.3:+19017126 MS.gene72989:CDS
ACCATTTCATTGCAAAATTG+GGG 0.509402 8.3:+19017718 MS.gene72989:CDS
TTCTCCAATTCCCAAAACTC+TGG 0.510256 8.3:-19018568 None:intergenic
CAACAGCACTTACTTTGGGT+TGG 0.512066 8.3:+19018720 MS.gene72989:CDS
TCCTCTATCTTGATCCTCAT+CGG 0.513315 8.3:-19018482 None:intergenic
CATCGAATGAGATGCTGTTG+AGG 0.514579 8.3:+19017952 MS.gene72989:CDS
AGTTTGGCACAATTTGGAGC+TGG 0.514639 8.3:+19017240 MS.gene72989:CDS
GTCTGAGCAGTTGTTGCTAA+AGG 0.515399 8.3:-19017054 None:intergenic
GGTGCAGAAATCACTTCAGC+AGG 0.516593 8.3:-19017366 None:intergenic
GCAGCAGCCGTTGAGGCCGG+TGG 0.517483 8.3:+19016954 MS.gene72989:CDS
GCATGGAGGGTTCAGATGCA+TGG 0.518052 8.3:+19018604 MS.gene72989:CDS
CATGATGATGTTGATGTTGG+TGG 0.518083 8.3:-19017458 None:intergenic
ACTTGGGAATTGGTGTAACC+TGG 0.522388 8.3:-19017917 None:intergenic
AGGAGAAGAGGACGAAGAAG+AGG 0.523774 8.3:+19017617 MS.gene72989:CDS
TTTGAATTGTTCTGGAGATG+AGG 0.524360 8.3:+19017326 MS.gene72989:CDS
CAGTGTTCACAAGCAGGAGC+AGG 0.525223 8.3:+19018691 MS.gene72989:CDS
CAACATCATCATGTCAATGA+AGG 0.526407 8.3:+19017468 MS.gene72989:CDS
ATTTACCTTGTAGCTTCCCT+CGG 0.526979 8.3:+19016911 MS.gene72989:CDS
AGGGTGGGAGGAGTTTGAAG+AGG 0.527118 8.3:+19017112 MS.gene72989:CDS
GACTACTGGATGAAAATCAA+AGG 0.528709 8.3:-19018174 None:intergenic
CAGCAGCCGTTGAGGCCGGT+GGG 0.533021 8.3:+19016955 MS.gene72989:CDS
TTCAAAGAGAAAGGAATGGA+AGG 0.533200 8.3:-19017309 None:intergenic
GTTACAGCAGATTCAAGCCA+AGG 0.534387 8.3:-19017408 None:intergenic
CTGACTCTCTGATGAACCAT+TGG 0.536463 8.3:-19017499 None:intergenic
TCCAAGCCATGTTCGAATCA+CGG 0.544215 8.3:+19018076 MS.gene72989:CDS
CATCAACACAACCAGTAAGC+AGG 0.548431 8.3:-19017665 None:intergenic
AGGAACATCGTTGCCTGCGA+AGG 0.549953 8.3:+19018524 MS.gene72989:CDS
GTGATTCGAACATGGCTTGG+AGG 0.550236 8.3:-19018074 None:intergenic
TAATTACAGTGTTCACAAGC+AGG 0.552347 8.3:+19018685 MS.gene72989:CDS
TCACTCCTTAATCTGTGAGT+TGG 0.553015 8.3:-19016826 None:intergenic
AAAACCAGCAAGTCTGTCTC+CGG 0.554388 8.3:-19018132 None:intergenic
ATTGTTCTGGAGATGAGGAA+AGG 0.554859 8.3:+19017331 MS.gene72989:CDS
CATGGAGGGTTCAGATGCAT+GGG 0.555361 8.3:+19018605 MS.gene72989:CDS
AGAAAGATGATAGTGGAGCA+TGG 0.555422 8.3:+19018587 MS.gene72989:CDS
ACATCGTTGCCTGCGAAGGA+AGG 0.555502 8.3:+19018528 MS.gene72989:CDS
GGAACACAATGAGGCGAGGT+TGG 0.556078 8.3:+19018379 MS.gene72989:CDS
GCTTTGGATGCTTCATGTGA+AGG 0.557282 8.3:+19018217 MS.gene72989:CDS
GATAGAGGAGATGTTAGCTA+GGG 0.557387 8.3:+19018496 MS.gene72989:CDS
AAAGGGAAGGTTCAATGCCT+CGG 0.557666 8.3:-19018156 None:intergenic
ATTGGGAGAGATCAAGGATG+AGG 0.561558 8.3:+19017077 MS.gene72989:CDS
TCAGAACATGAAGAGGGTAA+TGG 0.563710 8.3:+19017552 MS.gene72989:CDS
TCATCGGACTCTCCAGTGGA+AGG 0.563830 8.3:-19018466 None:intergenic
GCTAAACTTTGGAACAAGCT+AGG 0.565024 8.3:-19018038 None:intergenic
AAACCATAGACGATGTTGGC+TGG 0.565074 8.3:+19016778 None:intergenic
CCTTAGCCATCAGAGTCACT+AGG 0.567717 8.3:+19017799 MS.gene72989:CDS
CATCGTTGCCTGCGAAGGAA+GGG 0.568800 8.3:+19018529 MS.gene72989:CDS
GAATTGGAGAAAGATGATAG+TGG 0.568946 8.3:+19018580 MS.gene72989:CDS
TGACATGATGATGTTGATGT+TGG 0.569622 8.3:-19017461 None:intergenic
ACTACTGGATGAAAATCAAA+GGG 0.570042 8.3:-19018173 None:intergenic
CCACACCCGTGATTCGAACA+TGG 0.572154 8.3:-19018082 None:intergenic
TTGCCTGCGAAGGAAGGGAC+AGG 0.572346 8.3:+19018534 MS.gene72989:CDS
TGAAGTGGAGACAGGAGAAG+AGG 0.572761 8.3:+19017605 MS.gene72989:CDS
CGCGAAGAACCGTCTTTCCG+AGG 0.572785 8.3:-19016928 None:intergenic
TGTAGCTTCCCTCGGAAAGA+CGG 0.573619 8.3:+19016919 MS.gene72989:CDS
CCTAGTGACTCTGATGGCTA+AGG 0.575424 8.3:-19017799 None:intergenic
AGATGATAGTGGAGCATGGA+GGG 0.575789 8.3:+19018591 MS.gene72989:CDS
GGCAATGAATGAATTCAAGC+TGG 0.576427 8.3:-19018818 None:intergenic
TCTTTCCGAGGGAAGCTACA+AGG 0.578020 8.3:-19016916 None:intergenic
AAGACACTTCATGATGGTAG+TGG 0.578818 8.3:+19018281 MS.gene72989:CDS
ACAATTCAAAGAGAAAGGAA+TGG 0.578869 8.3:-19017313 None:intergenic
GATGTGAAATGAAGAAACTT+GGG 0.580703 8.3:-19017933 None:intergenic
TCAGCATGAAGGAACTGAAG+TGG 0.582854 8.3:+19017590 MS.gene72989:CDS
TAGGATTGGTGCTACGAATG+AGG 0.584060 8.3:-19018328 None:intergenic
GATATCCCCACCGGCCTCAA+CGG 0.585808 8.3:-19016961 None:intergenic
ATCCTCATCGGACTCTCCAG+TGG 0.587086 8.3:-19018470 None:intergenic
ATGCATGGGAGTCACTGATA+GGG 0.590517 8.3:+19018619 MS.gene72989:CDS
AGCCAAGGATTGTTAGACAA+AGG 0.592331 8.3:-19017393 None:intergenic
AGCAGCCGTTGAGGCCGGTG+GGG 0.594224 8.3:+19016956 MS.gene72989:CDS
CCATTTCATTGCAAAATTGG+GGG 0.595375 8.3:+19017719 MS.gene72989:CDS
TGTTCGAATCACGGGTGTGG+GGG 0.596570 8.3:+19018085 MS.gene72989:CDS
ATGTTCGAATCACGGGTGTG+GGG 0.598829 8.3:+19018084 MS.gene72989:CDS
CCAAAGCCTAGTGACTCTGA+TGG 0.602086 8.3:-19017805 None:intergenic
CTGCGAAGGAAGGGACAGGT+TGG 0.606995 8.3:+19018538 MS.gene72989:CDS
GCAAGAAGCGGAACACAATG+AGG 0.609256 8.3:+19018370 MS.gene72989:CDS
CTGGATGAAAATCAAAGGGA+AGG 0.609858 8.3:-19018169 None:intergenic
CGCGTTCGCAGCAGCCGTTG+AGG 0.611606 8.3:+19016947 MS.gene72989:CDS
GTGTTGATGCTATTGGGTCA+AGG 0.614329 8.3:+19017679 MS.gene72989:CDS
GGGAATCCTTATCAGCATGA+AGG 0.614596 8.3:+19017579 MS.gene72989:CDS
TCAAAGAGAAAGGAATGGAA+GGG 0.615189 8.3:-19017308 None:intergenic
GAATGCATCAGAACATGAAG+AGG 0.618408 8.3:+19017545 MS.gene72989:CDS
AATGAAGGTTCAGTCTCCAA+TGG 0.621452 8.3:+19017483 MS.gene72989:CDS
ACACTTCATGATGGTAGTGG+AGG 0.624635 8.3:+19018284 MS.gene72989:CDS
TGCTATTGGGTCAAGGAATG+TGG 0.631345 8.3:+19017686 MS.gene72989:CDS
CAAGAAGATACTGTGTAGAG+AGG 0.631657 8.3:-19018752 None:intergenic
ATGAAACTGTCACTGCATTG+AGG 0.631836 8.3:+19017886 MS.gene72989:CDS
GCGAAGAACCGTCTTTCCGA+GGG 0.631984 8.3:-19016927 None:intergenic
AAGGAAGGGACAGGTTGGAG+AGG 0.635039 8.3:+19018543 MS.gene72989:CDS
TTCTCCACTGATATCCCCAC+CGG 0.635563 8.3:-19016970 None:intergenic
ATCGACTTCGACATAAAGCA+AGG 0.635920 8.3:+19018005 MS.gene72989:CDS
AAGATGATAGTGGAGCATGG+AGG 0.636506 8.3:+19018590 MS.gene72989:CDS
GTTCACAAGCAGGAGCAGGA+AGG 0.645596 8.3:+19018695 MS.gene72989:CDS
AATGCATCAGAACATGAAGA+GGG 0.650132 8.3:+19017546 MS.gene72989:CDS
CTTCACAAGACACTTCATGA+TGG 0.664343 8.3:+19018275 MS.gene72989:CDS
TGAGCAGTTGTTGCTAAAGG+CGG 0.665701 8.3:-19017051 None:intergenic
GGATTGTTAGACAAAGGCAA+AGG 0.667360 8.3:-19017387 None:intergenic
ATGAAGAGGGTAATGGTTCG+GGG 0.669794 8.3:+19017559 MS.gene72989:CDS
TGTTATGGCAGAGCAAGAAG+CGG 0.671377 8.3:+19018358 MS.gene72989:CDS
ACATAAAGCAAGGACTACAA+TGG 0.673543 8.3:+19018015 MS.gene72989:CDS
GGGGATCTTGCATCTCCAAG+GGG 0.674372 8.3:+19017738 MS.gene72989:CDS
AAGCGGAACACAATGAGGCG+AGG 0.682806 8.3:+19018375 MS.gene72989:CDS
GAGGCCGGTGGGGATATCAG+TGG 0.691692 8.3:+19016966 MS.gene72989:CDS
TTCCACTGGAGAGTCCGATG+AGG 0.697132 8.3:+19018468 MS.gene72989:CDS
TTTGATTCGATTGATCACAG+TGG 0.727954 8.3:+19018443 MS.gene72989:CDS
GACGAAGAAGAGGAGCATCG+CGG 0.735685 8.3:+19017627 MS.gene72989:CDS
TGTGAAGGAACATGAAACTG+TGG 0.739109 8.3:+19018232 MS.gene72989:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAATTTTGATGAATTTGAA+AGG + chr8.3:19017208-19017227 MS.gene72989:CDS 20.0%
!!! GAATTTTGATGAATTTGAAA+GGG + chr8.3:19017209-19017228 MS.gene72989:CDS 20.0%
! AACCATTTCATTGCAAAATT+GGG + chr8.3:19017717-19017736 MS.gene72989:CDS 25.0%
AAATAGAGCTAAGAGGAAAA+AGG + chr8.3:19017170-19017189 MS.gene72989:CDS 30.0%
AAATGAAGAAACTTGGGAAT+TGG - chr8.3:19017930-19017949 None:intergenic 30.0%
AATACATCTTCAACAGAAAC+TGG - chr8.3:19018658-19018677 None:intergenic 30.0%
AATAGAGCTAAGAGGAAAAA+GGG + chr8.3:19017171-19017190 MS.gene72989:CDS 30.0%
ACAATTCAAAGAGAAAGGAA+TGG - chr8.3:19017316-19017335 None:intergenic 30.0%
ACCATTTCATTGCAAAATTG+GGG + chr8.3:19017718-19017737 MS.gene72989:CDS 30.0%
ACTACTGGATGAAAATCAAA+GGG - chr8.3:19018176-19018195 None:intergenic 30.0%
CAACCATTTCATTGCAAAAT+TGG + chr8.3:19017716-19017735 MS.gene72989:CDS 30.0%
CATAACAACAATTGTAGGAT+TGG - chr8.3:19018345-19018364 None:intergenic 30.0%
CGGTAAAATAAGCACAAATT+CGG - chr8.3:19017776-19017795 None:intergenic 30.0%
GATGTGAAATGAAGAAACTT+GGG - chr8.3:19017936-19017955 None:intergenic 30.0%
! CAATCCTACAATTGTTGTTA+TGG + chr8.3:19018343-19018362 MS.gene72989:CDS 30.0%
! GTAACAAAGATCACCAATTT+TGG + chr8.3:19017426-19017445 MS.gene72989:CDS 30.0%
AATGCATCAGAACATGAAGA+GGG + chr8.3:19017546-19017565 MS.gene72989:CDS 35.0%
ACATAAAGCAAGGACTACAA+TGG + chr8.3:19018015-19018034 MS.gene72989:CDS 35.0%
AGTTTCAGTTTGGCACAATT+TGG + chr8.3:19017234-19017253 MS.gene72989:CDS 35.0%
ATAAGCACAAATTCGGCTTA+TGG - chr8.3:19017769-19017788 None:intergenic 35.0%
ATGCATTCTCATTCAACCTT+AGG - chr8.3:19017533-19017552 None:intergenic 35.0%
CAACATCATCATGTCAATGA+AGG + chr8.3:19017468-19017487 MS.gene72989:CDS 35.0%
CCAGAACAATTCAAAGAGAA+AGG - chr8.3:19017321-19017340 None:intergenic 35.0%
CCATTTCATTGCAAAATTGG+GGG + chr8.3:19017719-19017738 MS.gene72989:CDS 35.0%
CCTTTCTCTTTGAATTGTTC+TGG + chr8.3:19017318-19017337 MS.gene72989:CDS 35.0%
CGATGTGAAATGAAGAAACT+TGG - chr8.3:19017937-19017956 None:intergenic 35.0%
GAATTGGAGAAAGATGATAG+TGG + chr8.3:19018580-19018599 MS.gene72989:CDS 35.0%
GACTACTGGATGAAAATCAA+AGG - chr8.3:19018177-19018196 None:intergenic 35.0%
TAATTACAGTGTTCACAAGC+AGG + chr8.3:19018685-19018704 MS.gene72989:CDS 35.0%
TCAAAGAGAAAGGAATGGAA+GGG - chr8.3:19017311-19017330 None:intergenic 35.0%
TCTGCCATAACAACAATTGT+AGG - chr8.3:19018350-19018369 None:intergenic 35.0%
TGAAAGGGAAAGTTTCAGTT+TGG + chr8.3:19017224-19017243 MS.gene72989:CDS 35.0%
TTCAAAGAGAAAGGAATGGA+AGG - chr8.3:19017312-19017331 None:intergenic 35.0%
! CTTTTGCAAATAGAGCTAAG+AGG + chr8.3:19017163-19017182 MS.gene72989:CDS 35.0%
! GTTCCAAAGTTTAGCTTCAA+GGG + chr8.3:19018046-19018065 MS.gene72989:CDS 35.0%
! TGACATGATGATGTTGATGT+TGG - chr8.3:19017464-19017483 None:intergenic 35.0%
! TGTTCCAAAGTTTAGCTTCA+AGG + chr8.3:19018045-19018064 MS.gene72989:CDS 35.0%
! TTTGATTCGATTGATCACAG+TGG + chr8.3:19018443-19018462 MS.gene72989:CDS 35.0%
! TTTTGGTCTGTTCACAGTTT+TGG + chr8.3:19017267-19017286 MS.gene72989:CDS 35.0%
!! AAAGATCACCAATTTTGGTG+AGG + chr8.3:19017431-19017450 MS.gene72989:CDS 35.0%
!! AAGATCACCAATTTTGGTGA+GGG + chr8.3:19017432-19017451 MS.gene72989:CDS 35.0%
!! AATTTGGAGCTGGAAACTTT+TGG + chr8.3:19017250-19017269 MS.gene72989:CDS 35.0%
!! TACTTCTCGTGATTTTGACA+GGG + chr8.3:19017851-19017870 MS.gene72989:CDS 35.0%
!! TTTGAATTGTTCTGGAGATG+AGG + chr8.3:19017326-19017345 MS.gene72989:CDS 35.0%
AAGACACTTCATGATGGTAG+TGG + chr8.3:19018281-19018300 MS.gene72989:CDS 40.0%
AAGCAAGAGCTGAATGAAAC+CGG + chr8.3:19018113-19018132 MS.gene72989:CDS 40.0%
AATCTCTGTGTGCTCATTGA+TGG - chr8.3:19016889-19016908 None:intergenic 40.0%
AATGAAGGTTCAGTCTCCAA+TGG + chr8.3:19017483-19017502 MS.gene72989:CDS 40.0%
AGATAGAGGAGATGTTAGCT+AGG + chr8.3:19018495-19018514 MS.gene72989:CDS 40.0%
AGATGTTAGCTAGGGAGATA+AGG + chr8.3:19018504-19018523 MS.gene72989:CDS 40.0%
AGCCAAGGATTGTTAGACAA+AGG - chr8.3:19017396-19017415 None:intergenic 40.0%
ATCAATGAGCACACAGAGAT+TGG + chr8.3:19016888-19016907 MS.gene72989:CDS 40.0%
ATCGAATGAGATGCTGTTGA+GGG + chr8.3:19017953-19017972 MS.gene72989:CDS 40.0%
ATGAAACTGTCACTGCATTG+AGG + chr8.3:19017886-19017905 MS.gene72989:CDS 40.0%
ATTTACCTTGTAGCTTCCCT+CGG + chr8.3:19016911-19016930 MS.gene72989:CDS 40.0%
CAAGAAGATACTGTGTAGAG+AGG - chr8.3:19018755-19018774 None:intergenic 40.0%
CTCTCTACACAGTATCTTCT+TGG + chr8.3:19018753-19018772 MS.gene72989:CDS 40.0%
CTGGATGAAAATCAAAGGGA+AGG - chr8.3:19018172-19018191 None:intergenic 40.0%
CTTCACAAGACACTTCATGA+TGG + chr8.3:19018275-19018294 MS.gene72989:CDS 40.0%
GAATGCATCAGAACATGAAG+AGG + chr8.3:19017545-19017564 MS.gene72989:CDS 40.0%
GACTTGAAGATGTAAGGCTT+TGG + chr8.3:19018201-19018220 MS.gene72989:CDS 40.0%
GATAGAGGAGATGTTAGCTA+GGG + chr8.3:19018496-19018515 MS.gene72989:CDS 40.0%
GCTAAGAGGAAAAAGGGTAA+TGG + chr8.3:19017177-19017196 MS.gene72989:CDS 40.0%
GGATTGTTAGACAAAGGCAA+AGG - chr8.3:19017390-19017409 None:intergenic 40.0%
TCACTCCTTAATCTGTGAGT+TGG - chr8.3:19016829-19016848 None:intergenic 40.0%
TCAGAACATGAAGAGGGTAA+TGG + chr8.3:19017552-19017571 MS.gene72989:CDS 40.0%
TCAGTTCCTTCATGCTGATA+AGG - chr8.3:19017588-19017607 None:intergenic 40.0%
TCCTCTATCTTGATCCTCAT+CGG - chr8.3:19018485-19018504 None:intergenic 40.0%
TCGACAGACTTGAAGATGTA+AGG + chr8.3:19018195-19018214 MS.gene72989:CDS 40.0%
TGCCTTTGTCTAACAATCCT+TGG + chr8.3:19017391-19017410 MS.gene72989:CDS 40.0%
TGTCACCAACTCACAGATTA+AGG + chr8.3:19016821-19016840 MS.gene72989:CDS 40.0%
TGTGAAGGAACATGAAACTG+TGG + chr8.3:19018232-19018251 MS.gene72989:CDS 40.0%
TTCTCCAATTCCCAAAACTC+TGG - chr8.3:19018571-19018590 None:intergenic 40.0%
! AAGAGGAAAAAGGGTAATGG+TGG + chr8.3:19017180-19017199 MS.gene72989:CDS 40.0%
! ACATGAAGAGGGTAATGGTT+CGG + chr8.3:19017557-19017576 MS.gene72989:CDS 40.0%
! ACTGGTTGTGTTGATGCTAT+TGG + chr8.3:19017672-19017691 MS.gene72989:CDS 40.0%
! AGATCAAGGATGAGGAGTTT+TGG + chr8.3:19017085-19017104 MS.gene72989:CDS 40.0%
! ATCGACTTCGACATAAAGCA+AGG + chr8.3:19018005-19018024 MS.gene72989:CDS 40.0%
! CATGATGATGTTGATGTTGG+TGG - chr8.3:19017461-19017480 None:intergenic 40.0%
! CCCCCAATTTTGCAATGAAA+TGG - chr8.3:19017722-19017741 None:intergenic 40.0%
! CTGGTTGTGTTGATGCTATT+GGG + chr8.3:19017673-19017692 MS.gene72989:CDS 40.0%
! GATCAAGGATGAGGAGTTTT+GGG + chr8.3:19017086-19017105 MS.gene72989:CDS 40.0%
!! AGAAAGATGATAGTGGAGCA+TGG + chr8.3:19018587-19018606 MS.gene72989:CDS 40.0%
!! ATTGTTCTGGAGATGAGGAA+AGG + chr8.3:19017331-19017350 MS.gene72989:CDS 40.0%
!! CTACTTCTCGTGATTTTGAC+AGG + chr8.3:19017850-19017869 MS.gene72989:CDS 40.0%
!! GCGCTGATTTGAAAAACATG+AGG - chr8.3:19017831-19017850 None:intergenic 40.0%
!! GCTAAACTTTGGAACAAGCT+AGG - chr8.3:19018041-19018060 None:intergenic 40.0%
!! GGCTTTTGAAGGGAAAGAAA+GGG + chr8.3:19017974-19017993 MS.gene72989:CDS 40.0%
!! TTGTTCTGGAGATGAGGAAA+GGG + chr8.3:19017332-19017351 MS.gene72989:CDS 40.0%
!!! AGGAGCATTGAGAGACTTTT+TGG + chr8.3:19018304-19018323 MS.gene72989:CDS 40.0%
!!! GGAGCATTGAGAGACTTTTT+GGG + chr8.3:19018305-19018324 MS.gene72989:CDS 40.0%
AAAACCAGCAAGTCTGTCTC+CGG - chr8.3:19018135-19018154 None:intergenic 45.0%
AAAGGGAAGGTTCAATGCCT+CGG - chr8.3:19018159-19018178 None:intergenic 45.0%
ACTTGGGAATTGGTGTAACC+TGG - chr8.3:19017920-19017939 None:intergenic 45.0%
AGAGTCAGAGTTTGAGCCTA+AGG + chr8.3:19017514-19017533 MS.gene72989:CDS 45.0%
AGCAACAACTGCTCAGACAT+TGG + chr8.3:19017059-19017078 MS.gene72989:CDS 45.0%
AGGAGCAACAGCACTTACTT+TGG + chr8.3:19018715-19018734 MS.gene72989:CDS 45.0%
ATGCATGGGAGTCACTGATA+GGG + chr8.3:19018619-19018638 MS.gene72989:CDS 45.0%
ATTGGGAGAGATCAAGGATG+AGG + chr8.3:19017077-19017096 MS.gene72989:CDS 45.0%
CAACAGCACTTACTTTGGGT+TGG + chr8.3:19018720-19018739 MS.gene72989:CDS 45.0%
CACAGTATCTTCTTGGACAC+CGG + chr8.3:19018760-19018779 MS.gene72989:CDS 45.0%
CATCAACACAACCAGTAAGC+AGG - chr8.3:19017668-19017687 None:intergenic 45.0%
CATCGAATGAGATGCTGTTG+AGG + chr8.3:19017952-19017971 MS.gene72989:CDS 45.0%
CTGACTCTCTGATGAACCAT+TGG - chr8.3:19017502-19017521 None:intergenic 45.0%
CTGCTGTTGTTCTTGACTTC+AGG - chr8.3:19016997-19017016 None:intergenic 45.0%
GCAACAACTGCTCAGACATT+GGG + chr8.3:19017060-19017079 MS.gene72989:CDS 45.0%
GCTTGCAGAACATGGATCTT+TGG + chr8.3:19017134-19017153 MS.gene72989:CDS 45.0%
GGAGCAACAGCACTTACTTT+GGG + chr8.3:19018716-19018735 MS.gene72989:CDS 45.0%
GGGAATCCTTATCAGCATGA+AGG + chr8.3:19017579-19017598 MS.gene72989:CDS 45.0%
GTCTGAGCAGTTGTTGCTAA+AGG - chr8.3:19017057-19017076 None:intergenic 45.0%
GTTACAGCAGATTCAAGCCA+AGG - chr8.3:19017411-19017430 None:intergenic 45.0%
GTTCTCTGCAGCAGAACATT+CGG + chr8.3:19017025-19017044 MS.gene72989:CDS 45.0%
TCAAGCTGGCTGAGAAAAAG+AGG - chr8.3:19018807-19018826 None:intergenic 45.0%
TCAGACATTGGGAGAGATCA+AGG + chr8.3:19017071-19017090 MS.gene72989:CDS 45.0%
TCAGCATGAAGGAACTGAAG+TGG + chr8.3:19017590-19017609 MS.gene72989:CDS 45.0%
TCCAAGCCATGTTCGAATCA+CGG + chr8.3:19018076-19018095 MS.gene72989:CDS 45.0%
TCCGATGAGGATCAAGATAG+AGG + chr8.3:19018481-19018500 MS.gene72989:CDS 45.0%
TGAGCAGTTGTTGCTAAAGG+CGG - chr8.3:19017054-19017073 None:intergenic 45.0%
TGCATTGAGGCTTCTAAACC+AGG + chr8.3:19017899-19017918 MS.gene72989:CDS 45.0%
TGCTATTGGGTCAAGGAATG+TGG + chr8.3:19017686-19017705 MS.gene72989:CDS 45.0%
TGGCCCTTGAAGCTAAACTT+TGG - chr8.3:19018052-19018071 None:intergenic 45.0%
TGTTATGGCAGAGCAAGAAG+CGG + chr8.3:19018358-19018377 MS.gene72989:CDS 45.0%
TTGAAGAGGCTTGCAGAACA+TGG + chr8.3:19017126-19017145 MS.gene72989:CDS 45.0%
! AGTTTGGCACAATTTGGAGC+TGG + chr8.3:19017240-19017259 MS.gene72989:CDS 45.0%
! ATGAAGAGGGTAATGGTTCG+GGG + chr8.3:19017559-19017578 MS.gene72989:CDS 45.0%
! ATGCTGTTGAGGGCTTTTGA+AGG + chr8.3:19017963-19017982 MS.gene72989:CDS 45.0%
! CATGAAGAGGGTAATGGTTC+GGG + chr8.3:19017558-19017577 MS.gene72989:CDS 45.0%
! GCTTTGGATGCTTCATGTGA+AGG + chr8.3:19018217-19018236 MS.gene72989:CDS 45.0%
! GTGTTGATGCTATTGGGTCA+AGG + chr8.3:19017679-19017698 MS.gene72989:CDS 45.0%
! TCTTCAAGTCTGTCGACTAC+TGG - chr8.3:19018191-19018210 None:intergenic 45.0%
! TGCTGTTGAGGGCTTTTGAA+GGG + chr8.3:19017964-19017983 MS.gene72989:CDS 45.0%
!! AAGATGATAGTGGAGCATGG+AGG + chr8.3:19018590-19018609 MS.gene72989:CDS 45.0%
!! ACACTTCATGATGGTAGTGG+AGG + chr8.3:19018284-19018303 MS.gene72989:CDS 45.0%
!! AGATGATAGTGGAGCATGGA+GGG + chr8.3:19018591-19018610 MS.gene72989:CDS 45.0%
!! GGGCTTTTGAAGGGAAAGAA+AGG + chr8.3:19017973-19017992 MS.gene72989:CDS 45.0%
!! TAGGATTGGTGCTACGAATG+AGG - chr8.3:19018331-19018350 None:intergenic 45.0%
!! TGGTCTGTTCACAGTTTTGG+AGG + chr8.3:19017270-19017289 MS.gene72989:CDS 45.0%
!! TTCTCGTGATTTTGACAGGG+TGG + chr8.3:19017854-19017873 MS.gene72989:CDS 45.0%
!!! GATGAGGAGTTTTGGGAGAA+GGG + chr8.3:19017093-19017112 MS.gene72989:CDS 45.0%
!!! GGTCTGTTCACAGTTTTGGA+GGG + chr8.3:19017271-19017290 MS.gene72989:CDS 45.0%
AGGAGAAGAGGACGAAGAAG+AGG + chr8.3:19017617-19017636 MS.gene72989:CDS 50.0%
ATGTTCGAATCACGGGTGTG+GGG + chr8.3:19018084-19018103 MS.gene72989:CDS 50.0%
CATGGAGGGTTCAGATGCAT+GGG + chr8.3:19018605-19018624 MS.gene72989:CDS 50.0%
CATGTTCGAATCACGGGTGT+GGG + chr8.3:19018083-19018102 MS.gene72989:CDS 50.0%
CCAAAGCCTAGTGACTCTGA+TGG - chr8.3:19017808-19017827 None:intergenic 50.0%
CCAAGCCATGTTCGAATCAC+GGG + chr8.3:19018077-19018096 MS.gene72989:CDS 50.0%
CCATCAGAGTCACTAGGCTT+TGG + chr8.3:19017805-19017824 MS.gene72989:CDS 50.0%
CCTAGTGACTCTGATGGCTA+AGG - chr8.3:19017802-19017821 None:intergenic 50.0%
CCTTAGCCATCAGAGTCACT+AGG + chr8.3:19017799-19017818 MS.gene72989:CDS 50.0%
GAAGGAACTGAAGTGGAGAC+AGG + chr8.3:19017597-19017616 MS.gene72989:CDS 50.0%
GACTCTGATGGCTAAGGCTT+CGG - chr8.3:19017796-19017815 None:intergenic 50.0%
GATGCATGGGAGTCACTGAT+AGG + chr8.3:19018618-19018637 MS.gene72989:CDS 50.0%
GCAAGAAGCGGAACACAATG+AGG + chr8.3:19018370-19018389 MS.gene72989:CDS 50.0%
GGTGCAGAAATCACTTCAGC+AGG - chr8.3:19017369-19017388 None:intergenic 50.0%
GTGGCTACCCTCACCAAAAT+TGG - chr8.3:19017442-19017461 None:intergenic 50.0%
TCTTTCCGAGGGAAGCTACA+AGG - chr8.3:19016919-19016938 None:intergenic 50.0%
TGAAGTGGAGACAGGAGAAG+AGG + chr8.3:19017605-19017624 MS.gene72989:CDS 50.0%
TGTAGCTTCCCTCGGAAAGA+CGG + chr8.3:19016919-19016938 MS.gene72989:CDS 50.0%
TTCTCCACTGATATCCCCAC+CGG - chr8.3:19016973-19016992 None:intergenic 50.0%
! GAGCTTTTGAGCCTGCTTAC+TGG + chr8.3:19017654-19017673 MS.gene72989:CDS 50.0%
! GTGATTCGAACATGGCTTGG+AGG - chr8.3:19018077-19018096 None:intergenic 50.0%
! TCGAACATGGCTTGGAGGAT+TGG - chr8.3:19018072-19018091 None:intergenic 50.0%
!! TCGTGATTTTGACAGGGTGG+TGG + chr8.3:19017857-19017876 MS.gene72989:CDS 50.0%
!!! AGGAGTTTTGGGAGAAGGGT+GGG + chr8.3:19017097-19017116 MS.gene72989:CDS 50.0%
!!! GGATGAGGAGTTTTGGGAGA+AGG + chr8.3:19017092-19017111 MS.gene72989:CDS 50.0%
!!! GGCACCAGAGTTTTGGGAAT+TGG + chr8.3:19018564-19018583 MS.gene72989:CDS 50.0%
!!! TGGAGAGGCACCAGAGTTTT+GGG + chr8.3:19018558-19018577 MS.gene72989:CDS 50.0%
!!! TTGGAGAGGCACCAGAGTTT+TGG + chr8.3:19018557-19018576 MS.gene72989:CDS 50.0%
AAGCGGAACACAATGAGGCG+AGG + chr8.3:19018375-19018394 MS.gene72989:CDS 55.0%
ACATCGTTGCCTGCGAAGGA+AGG + chr8.3:19018528-19018547 MS.gene72989:CDS 55.0%
AGGAACATCGTTGCCTGCGA+AGG + chr8.3:19018524-19018543 MS.gene72989:CDS 55.0%
AGGGTGGGAGGAGTTTGAAG+AGG + chr8.3:19017112-19017131 MS.gene72989:CDS 55.0%
ATCCTCATCGGACTCTCCAG+TGG - chr8.3:19018473-19018492 None:intergenic 55.0%
CAGTGTTCACAAGCAGGAGC+AGG + chr8.3:19018691-19018710 MS.gene72989:CDS 55.0%
CATCGTTGCCTGCGAAGGAA+GGG + chr8.3:19018529-19018548 MS.gene72989:CDS 55.0%
CCACACCCGTGATTCGAACA+TGG - chr8.3:19018085-19018104 None:intergenic 55.0%
CCATGTTCGAATCACGGGTG+TGG + chr8.3:19018082-19018101 MS.gene72989:CDS 55.0%
CCCGTGATTCGAACATGGCT+TGG - chr8.3:19018080-19018099 None:intergenic 55.0%
CGGCTTATGGAAGTTCCCCT+TGG - chr8.3:19017756-19017775 None:intergenic 55.0%
GAAACCGGAGACAGACTTGC+TGG + chr8.3:19018128-19018147 MS.gene72989:CDS 55.0%
GAGCTTCCAGCAACGTCAAC+CGG - chr8.3:19018782-19018801 None:intergenic 55.0%
GCATGGAGGGTTCAGATGCA+TGG + chr8.3:19018604-19018623 MS.gene72989:CDS 55.0%
GCGAAGAACCGTCTTTCCGA+GGG - chr8.3:19016930-19016949 None:intergenic 55.0%
GGAACACAATGAGGCGAGGT+TGG + chr8.3:19018379-19018398 MS.gene72989:CDS 55.0%
GGGGATCTTGCATCTCCAAG+GGG + chr8.3:19017738-19017757 MS.gene72989:CDS 55.0%
GGGGGATCTTGCATCTCCAA+GGG + chr8.3:19017737-19017756 MS.gene72989:CDS 55.0%
GTTCACAAGCAGGAGCAGGA+AGG + chr8.3:19018695-19018714 MS.gene72989:CDS 55.0%
TCATCGGACTCTCCAGTGGA+AGG - chr8.3:19018469-19018488 None:intergenic 55.0%
TGATCACAGTGGCCTTCCAC+TGG + chr8.3:19018454-19018473 MS.gene72989:CDS 55.0%
TGGGGGATCTTGCATCTCCA+AGG + chr8.3:19017736-19017755 MS.gene72989:CDS 55.0%
TGTTCGAATCACGGGTGTGG+GGG + chr8.3:19018085-19018104 MS.gene72989:CDS 55.0%
TTCCACTGGAGAGTCCGATG+AGG + chr8.3:19018468-19018487 MS.gene72989:CDS 55.0%
! AAGGAAGGGACAGGTTGGAG+AGG + chr8.3:19018543-19018562 MS.gene72989:CDS 55.0%
!! GACGAAGAAGAGGAGCATCG+CGG + chr8.3:19017627-19017646 MS.gene72989:CDS 55.0%
!!! AGTTTTGGGAGAAGGGTGGG+AGG + chr8.3:19017100-19017119 MS.gene72989:CDS 55.0%
!!! CAGACTTGCTGGTTTTGCCG+AGG + chr8.3:19018139-19018158 MS.gene72989:CDS 55.0%
!!! GAGGAGTTTTGGGAGAAGGG+TGG + chr8.3:19017096-19017115 MS.gene72989:CDS 55.0%
CAACCTGTCCCTTCCTTCGC+AGG - chr8.3:19018540-19018559 None:intergenic 60.0%
CGCGAAGAACCGTCTTTCCG+AGG - chr8.3:19016931-19016950 None:intergenic 60.0%
CTGCGAAGGAAGGGACAGGT+TGG + chr8.3:19018538-19018557 MS.gene72989:CDS 60.0%
GATATCCCCACCGGCCTCAA+CGG - chr8.3:19016964-19016983 None:intergenic 60.0%
TTGCCTGCGAAGGAAGGGAC+AGG + chr8.3:19018534-19018553 MS.gene72989:CDS 60.0%
! TGGACACCGGTTGACGTTGC+TGG + chr8.3:19018773-19018792 MS.gene72989:CDS 60.0%
GAGGCCGGTGGGGATATCAG+TGG + chr8.3:19016966-19016985 MS.gene72989:CDS 65.0%
TTCGCAGCAGCCGTTGAGGC+CGG + chr8.3:19016951-19016970 MS.gene72989:CDS 65.0%
AGCAGCCGTTGAGGCCGGTG+GGG + chr8.3:19016956-19016975 MS.gene72989:CDS 70.0%
CAGCAGCCGTTGAGGCCGGT+GGG + chr8.3:19016955-19016974 MS.gene72989:CDS 70.0%
CGCGTTCGCAGCAGCCGTTG+AGG + chr8.3:19016947-19016966 MS.gene72989:CDS 70.0%
GCAGCAGCCGTTGAGGCCGG+TGG + chr8.3:19016954-19016973 MS.gene72989:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 19016790 19018826 19016790 ID=MS.gene72989
chr8.3 mRNA 19016790 19018826 19016790 ID=MS.gene72989.t1;Parent=MS.gene72989
chr8.3 exon 19016790 19018826 19016790 ID=MS.gene72989.t1.exon1;Parent=MS.gene72989.t1
chr8.3 CDS 19016790 19018826 19016790 ID=cds.MS.gene72989.t1;Parent=MS.gene72989.t1
Gene Sequence

>MS.gene72989

ATGTTGGCTGGTTGTTCTAGCTCGACATTGTTGTCACCAACTCACAGATTAAGGAGTGAAGCATCAGCACAGTTTCAAGCTTGTCATTTTCAGCTACCATCAATGAGCACACAGAGATTGGATTTACCTTGTAGCTTCCCTCGGAAAGACGGTTCTTCGCGTTCGCAGCAGCCGTTGAGGCCGGTGGGGATATCAGTGGAGAAGCCTGAAGTCAAGAACAACAGCAGTAGCACTTGTTCTCTGCAGCAGAACATTCGGTTACCGCCTTTAGCAACAACTGCTCAGACATTGGGAGAGATCAAGGATGAGGAGTTTTGGGAGAAGGGTGGGAGGAGTTTGAAGAGGCTTGCAGAACATGGATCTTTGGATGAGTCTTTTGCAAATAGAGCTAAGAGGAAAAAGGGTAATGGTGGTGATGAGAATTTTGATGAATTTGAAAGGGAAAGTTTCAGTTTGGCACAATTTGGAGCTGGAAACTTTTGGTCTGTTCACAGTTTTGGAGGGTTTAATAGTAATAGCCCTTCCATTCCTTTCTCTTTGAATTGTTCTGGAGATGAGGAAAGGGTTTGTTTTGCTCCTGCTGAAGTGATTTCTGCACCTTTGCCTTTGTCTAACAATCCTTGGCTTGAATCTGCTGTAACAAAGATCACCAATTTTGGTGAGGGTAGCCACCAACATCAACATCATCATGTCAATGAAGGTTCAGTCTCCAATGGTTCATCAGAGAGTCAGAGTTTGAGCCTAAGGTTGAATGAGAATGCATCAGAACATGAAGAGGGTAATGGTTCGGGGAATCCTTATCAGCATGAAGGAACTGAAGTGGAGACAGGAGAAGAGGACGAAGAAGAGGAGCATCGCGGTTTTGAGCTTTTGAGCCTGCTTACTGGTTGTGTTGATGCTATTGGGTCAAGGAATGTGGCTGCAGTCAACCATTTCATTGCAAAATTGGGGGATCTTGCATCTCCAAGGGGAACTTCCATAAGCCGAATTTGTGCTTATTTTACCGAAGCCTTAGCCATCAGAGTCACTAGGCTTTGGCCTCATGTTTTTCAAATCAGCGCTACTTCTCGTGATTTTGACAGGGTGGTGGATGATGATGAAACTGTCACTGCATTGAGGCTTCTAAACCAGGTTACACCAATTCCCAAGTTTCTTCATTTCACATCGAATGAGATGCTGTTGAGGGCTTTTGAAGGGAAAGAAAGGGTTCACATTATCGACTTCGACATAAAGCAAGGACTACAATGGCCTAGCTTGTTCCAAAGTTTAGCTTCAAGGGCCAATCCTCCAAGCCATGTTCGAATCACGGGTGTGGGGGAATCGAAGCAAGAGCTGAATGAAACCGGAGACAGACTTGCTGGTTTTGCCGAGGCATTGAACCTTCCCTTTGATTTTCATCCAGTAGTCGACAGACTTGAAGATGTAAGGCTTTGGATGCTTCATGTGAAGGAACATGAAACTGTGGCTGTGAACTGTGTTTTGCAGCTTCACAAGACACTTCATGATGGTAGTGGAGGAGCATTGAGAGACTTTTTGGGCCTCATTCGTAGCACCAATCCTACAATTGTTGTTATGGCAGAGCAAGAAGCGGAACACAATGAGGCGAGGTTGGACTCAAGAGTCTGCAATTCATTGAAATATTACTCAGCGCTGTTTGATTCGATTGATCACAGTGGCCTTCCACTGGAGAGTCCGATGAGGATCAAGATAGAGGAGATGTTAGCTAGGGAGATAAGGAACATCGTTGCCTGCGAAGGAAGGGACAGGTTGGAGAGGCACCAGAGTTTTGGGAATTGGAGAAAGATGATAGTGGAGCATGGAGGGTTCAGATGCATGGGAGTCACTGATAGGGAACTGATTCAGAGCCAGTTTCTGTTGAAGATGTATTCTTGTGATAATTACAGTGTTCACAAGCAGGAGCAGGAAGGAGCAACAGCACTTACTTTGGGTTGGCTTGATCAACCTCTCTACACAGTATCTTCTTGGACACCGGTTGACGTTGCTGGAAGCTCATCCTCTTTTTCTCAGCCAGCTTGA
Protein sequence

>MS.gene72989.t1

MLAGCSSSTLLSPTHRLRSEASAQFQACHFQLPSMSTQRLDLPCSFPRKDGSSRSQQPLRPVGISVEKPEVKNNSSSTCSLQQNIRLPPLATTAQTLGEIKDEEFWEKGGRSLKRLAEHGSLDESFANRAKRKKGNGGDENFDEFERESFSLAQFGAGNFWSVHSFGGFNSNSPSIPFSLNCSGDEERVCFAPAEVISAPLPLSNNPWLESAVTKITNFGEGSHQHQHHHVNEGSVSNGSSESQSLSLRLNENASEHEEGNGSGNPYQHEGTEVETGEEDEEEEHRGFELLSLLTGCVDAIGSRNVAAVNHFIAKLGDLASPRGTSISRICAYFTEALAIRVTRLWPHVFQISATSRDFDRVVDDDETVTALRLLNQVTPIPKFLHFTSNEMLLRAFEGKERVHIIDFDIKQGLQWPSLFQSLASRANPPSHVRITGVGESKQELNETGDRLAGFAEALNLPFDFHPVVDRLEDVRLWMLHVKEHETVAVNCVLQLHKTLHDGSGGALRDFLGLIRSTNPTIVVMAEQEAEHNEARLDSRVCNSLKYYSALFDSIDHSGLPLESPMRIKIEEMLAREIRNIVACEGRDRLERHQSFGNWRKMIVEHGGFRCMGVTDRELIQSQFLLKMYSCDNYSVHKQEQEGATALTLGWLDQPLYTVSSWTPVDVAGSSSSFSQPA