Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73015.t1 | XP_013457586.1 | 97.8 | 136 | 3 | 0 | 1 | 136 | 1207 | 1342 | 1.60E-66 | 261.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73015.t1 | A0A072UPJ3 | 97.8 | 136 | 3 | 0 | 1 | 136 | 1207 | 1342 | 1.2e-66 | 261.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene73015 | 0.816645 | 4.97E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene73015 | 0.803709 | 3.06E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene73015 | 0.800972 | 1.12E-48 | -1.69E-46 |
| MS.gene052582 | MS.gene73015 | 0.813969 | 1.96E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene73015 | 0.81028 | 1.25E-50 | -1.69E-46 |
| MS.gene053122 | MS.gene73015 | 0.801041 | 1.09E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene73015 | 0.82228 | 2.58E-53 | -1.69E-46 |
| MS.gene056215 | MS.gene73015 | 0.802935 | 4.43E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73015.t1 | MTR_4g099300 | 97.794 | 136 | 3 | 0 | 1 | 136 | 1207 | 1342 | 3.00e-84 | 268 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73015.t1 | AT4G17610 | 72.059 | 136 | 38 | 0 | 1 | 136 | 1189 | 1324 | 1.91e-58 | 195 |
| MS.gene73015.t1 | AT4G17610 | 72.059 | 136 | 38 | 0 | 1 | 136 | 1189 | 1324 | 2.07e-58 | 195 |
| MS.gene73015.t1 | AT4G17610 | 72.059 | 136 | 38 | 0 | 1 | 136 | 1190 | 1325 | 2.07e-58 | 195 |
Find 26 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCTGCATTGCACTTAAC+AGG | 0.241113 | 8.1:-23005467 | MS.gene73015:CDS |
| TGCATTGCTGCATTGGATTC+CGG | 0.291235 | 8.1:-23004665 | MS.gene73015:CDS |
| AAATAAAACTTTATAATCAT+AGG | 0.319767 | 8.1:+23005428 | None:intergenic |
| CACTAATTGCATTGCTGCAT+TGG | 0.358967 | 8.1:-23004672 | MS.gene73015:CDS |
| CATTGCACTTAACAGGGTTA+TGG | 0.361509 | 8.1:-23005460 | MS.gene73015:CDS |
| ACTTCTGTCAGCTCAGGATC+AGG | 0.362013 | 8.1:+23005188 | None:intergenic |
| CTTAAACTGCTCTCACTCTA+TGG | 0.372616 | 8.1:-23005398 | MS.gene73015:CDS |
| CACTAGCAGAAATGATTTGC+CGG | 0.404777 | 8.1:+23004646 | None:intergenic |
| CTGCTCTCACTCTATGGTTC+AGG | 0.407786 | 8.1:-23005392 | MS.gene73015:intron |
| GTTGACAGACTTGGCTTGTA+TGG | 0.430480 | 8.1:-23004714 | MS.gene73015:CDS |
| CTGGAGGTCTCTTTATTAGC+CGG | 0.454246 | 8.1:-23005215 | MS.gene73015:CDS |
| TTGCTTACTTCTGTCAGCTC+AGG | 0.454437 | 8.1:+23005182 | None:intergenic |
| ATGCAATTAGTGTCTTCGTT+TGG | 0.463607 | 8.1:+23004683 | None:intergenic |
| ACAGACTTGGCTTGTATGGT+TGG | 0.481974 | 8.1:-23004710 | MS.gene73015:CDS |
| ATAGAAAATCTTCTTGAGGA+AGG | 0.498547 | 8.1:-23005515 | MS.gene73015:intron |
| TGGAGGTCTCTTTATTAGCC+GGG | 0.505487 | 8.1:-23005214 | MS.gene73015:CDS |
| TCTTTCAGTTGCATTTGATG+AGG | 0.522691 | 8.1:-23005276 | MS.gene73015:intron |
| TGACAATAATGATGCTAGGC+TGG | 0.535396 | 8.1:-23005234 | MS.gene73015:CDS |
| CTTCTGTCAGCTCAGGATCA+GGG | 0.540220 | 8.1:+23005189 | None:intergenic |
| CAGCTCAGGATCAGGGCTCC+CGG | 0.542501 | 8.1:+23005196 | None:intergenic |
| GCAGCAAGTCGAATTGTTCG+AGG | 0.563052 | 8.1:+23005482 | None:intergenic |
| TAGCTGACAATAATGATGCT+AGG | 0.577477 | 8.1:-23005238 | MS.gene73015:CDS |
| TTGCTGCATTGCACTTAACA+GGG | 0.596202 | 8.1:-23005466 | MS.gene73015:CDS |
| GTTTATAGAAAATCTTCTTG+AGG | 0.600708 | 8.1:-23005519 | MS.gene73015:intron |
| CAATAATGATGCTAGGCTGG+AGG | 0.660874 | 8.1:-23005231 | MS.gene73015:CDS |
| GCTAGTGCTTCTTGACTCTG+CGG | 0.674810 | 8.1:-23004630 | MS.gene73015:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAACTTTATAATCAT+AGG | + | chr8.1:23004735-23004754 | None:intergenic | 10.0% |
| !! | TATAATTATCACATGAATAT+TGG | - | chr8.1:23004856-23004875 | MS.gene73015:intron | 15.0% |
| !!! | ATTTGATTATGTTATTTGAT+GGG | - | chr8.1:23005048-23005067 | MS.gene73015:intron | 15.0% |
| !!! | TATTTGATTATGTTATTTGA+TGG | - | chr8.1:23005047-23005066 | MS.gene73015:intron | 15.0% |
| !! | AATCAGATTTAACCAAAATT+TGG | - | chr8.1:23005176-23005195 | MS.gene73015:intron | 20.0% |
| !!! | AAATTAAAGTAGCCAAATTT+TGG | + | chr8.1:23005191-23005210 | None:intergenic | 20.0% |
| ! | ATACAGCATATAAACATGTT+TGG | + | chr8.1:23004796-23004815 | None:intergenic | 25.0% |
| ! | ATTCGATGTTTAAGAAATTC+GGG | + | chr8.1:23005226-23005245 | None:intergenic | 25.0% |
| ! | CATTCGATGTTTAAGAAATT+CGG | + | chr8.1:23005227-23005246 | None:intergenic | 25.0% |
| ! | GTTTATAGAAAATCTTCTTG+AGG | - | chr8.1:23004641-23004660 | MS.gene73015:CDS | 25.0% |
| ! | TAATGACAATAAGCATATCA+TGG | + | chr8.1:23005273-23005292 | None:intergenic | 25.0% |
| !! | GTATCATTTTGCAATTTACT+TGG | - | chr8.1:23004812-23004831 | MS.gene73015:intron | 25.0% |
| !!! | TGACTGTTTTTTTTAATGTC+TGG | - | chr8.1:23004613-23004632 | MS.gene73015:intron | 25.0% |
| AATTAGAAGAGAAGAGATAC+GGG | + | chr8.1:23004582-23004601 | None:intergenic | 30.0% | |
| AATTGGTCATTCTTCTTATC+AGG | - | chr8.1:23005368-23005387 | MS.gene73015:intron | 30.0% | |
| ATAGAAAATCTTCTTGAGGA+AGG | - | chr8.1:23004645-23004664 | MS.gene73015:CDS | 30.0% | |
| GAAGCAAGATCTCAAAATAT+TGG | + | chr8.1:23005117-23005136 | None:intergenic | 30.0% | |
| GAATTAGAAGAGAAGAGATA+CGG | + | chr8.1:23004583-23004602 | None:intergenic | 30.0% | |
| TAATAACTGGTCGTACTAAT+TGG | - | chr8.1:23005351-23005370 | MS.gene73015:intron | 30.0% | |
| TCATAATACACAAGAAGCAA+AGG | + | chr8.1:23005139-23005158 | None:intergenic | 30.0% | |
| !!! | GCTTTTGACTTTGTAATAAC+TGG | - | chr8.1:23005338-23005357 | MS.gene73015:intron | 30.0% |
| ATGCAATTAGTGTCTTCGTT+TGG | + | chr8.1:23005480-23005499 | None:intergenic | 35.0% | |
| TAGCTGACAATAATGATGCT+AGG | - | chr8.1:23004922-23004941 | MS.gene73015:intron | 35.0% | |
| TCTTTCAGTTGCATTTGATG+AGG | - | chr8.1:23004884-23004903 | MS.gene73015:intron | 35.0% | |
| ! | CTTGTTTACATTGATAGCCT+GGG | - | chr8.1:23005074-23005093 | MS.gene73015:intron | 35.0% |
| ! | GCATTTGATGAGGATTTTGA+AGG | - | chr8.1:23004894-23004913 | MS.gene73015:intron | 35.0% |
| ! | TTGTTTACATTGATAGCCTG+GGG | - | chr8.1:23005075-23005094 | MS.gene73015:intron | 35.0% |
| !! | GTTTTACAAGTTGACAGACT+TGG | - | chr8.1:23005437-23005456 | MS.gene73015:CDS | 35.0% |
| CACTAATTGCATTGCTGCAT+TGG | - | chr8.1:23005488-23005507 | MS.gene73015:CDS | 40.0% | |
| CATTGCACTTAACAGGGTTA+TGG | - | chr8.1:23004700-23004719 | MS.gene73015:CDS | 40.0% | |
| CCAGGCTATCAATGTAAACA+AGG | + | chr8.1:23005076-23005095 | None:intergenic | 40.0% | |
| CTTAAACTGCTCTCACTCTA+TGG | - | chr8.1:23004762-23004781 | MS.gene73015:CDS | 40.0% | |
| TATCAAATCAAATCAGCCCC+AGG | + | chr8.1:23005094-23005113 | None:intergenic | 40.0% | |
| TGACAATAATGATGCTAGGC+TGG | - | chr8.1:23004926-23004945 | MS.gene73015:intron | 40.0% | |
| TTGCTGCATTGCACTTAACA+GGG | - | chr8.1:23004694-23004713 | MS.gene73015:CDS | 40.0% | |
| ! | CACTAGCAGAAATGATTTGC+CGG | + | chr8.1:23005517-23005536 | None:intergenic | 40.0% |
| ! | CCTTGTTTACATTGATAGCC+TGG | - | chr8.1:23005073-23005092 | MS.gene73015:intron | 40.0% |
| CAATAATGATGCTAGGCTGG+AGG | - | chr8.1:23004929-23004948 | MS.gene73015:intron | 45.0% | |
| CTGGAGGTCTCTTTATTAGC+CGG | - | chr8.1:23004945-23004964 | MS.gene73015:intron | 45.0% | |
| CTTGCTGCATTGCACTTAAC+AGG | - | chr8.1:23004693-23004712 | MS.gene73015:CDS | 45.0% | |
| TGCATTGCTGCATTGGATTC+CGG | - | chr8.1:23005495-23005514 | MS.gene73015:CDS | 45.0% | |
| TGGAGGTCTCTTTATTAGCC+GGG | - | chr8.1:23004946-23004965 | MS.gene73015:intron | 45.0% | |
| TTGCTTACTTCTGTCAGCTC+AGG | + | chr8.1:23004981-23005000 | None:intergenic | 45.0% | |
| !! | ACAGACTTGGCTTGTATGGT+TGG | - | chr8.1:23005450-23005469 | MS.gene73015:CDS | 45.0% |
| !! | GTTGACAGACTTGGCTTGTA+TGG | - | chr8.1:23005446-23005465 | MS.gene73015:CDS | 45.0% |
| ACTTCTGTCAGCTCAGGATC+AGG | + | chr8.1:23004975-23004994 | None:intergenic | 50.0% | |
| CTGCTCTCACTCTATGGTTC+AGG | - | chr8.1:23004768-23004787 | MS.gene73015:CDS | 50.0% | |
| CTTCTGTCAGCTCAGGATCA+GGG | + | chr8.1:23004974-23004993 | None:intergenic | 50.0% | |
| GCAGCAAGTCGAATTGTTCG+AGG | + | chr8.1:23004681-23004700 | None:intergenic | 50.0% | |
| GCTAGTGCTTCTTGACTCTG+CGG | - | chr8.1:23005530-23005549 | MS.gene73015:intron | 50.0% | |
| CAGCTCAGGATCAGGGCTCC+CGG | + | chr8.1:23004967-23004986 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 23004545 | 23005637 | 23004545 | ID=MS.gene73015 |
| chr8.1 | mRNA | 23004545 | 23005637 | 23004545 | ID=MS.gene73015.t1;Parent=MS.gene73015 |
| chr8.1 | exon | 23005634 | 23005637 | 23005634 | ID=MS.gene73015.t1.exon1;Parent=MS.gene73015.t1 |
| chr8.1 | CDS | 23005634 | 23005637 | 23005634 | ID=cds.MS.gene73015.t1;Parent=MS.gene73015.t1 |
| chr8.1 | exon | 23005393 | 23005533 | 23005393 | ID=MS.gene73015.t1.exon2;Parent=MS.gene73015.t1 |
| chr8.1 | CDS | 23005393 | 23005533 | 23005393 | ID=cds.MS.gene73015.t1;Parent=MS.gene73015.t1 |
| chr8.1 | exon | 23005190 | 23005290 | 23005190 | ID=MS.gene73015.t1.exon3;Parent=MS.gene73015.t1 |
| chr8.1 | CDS | 23005190 | 23005290 | 23005190 | ID=cds.MS.gene73015.t1;Parent=MS.gene73015.t1 |
| chr8.1 | exon | 23004631 | 23004792 | 23004631 | ID=MS.gene73015.t1.exon4;Parent=MS.gene73015.t1 |
| chr8.1 | CDS | 23004631 | 23004792 | 23004631 | ID=cds.MS.gene73015.t1;Parent=MS.gene73015.t1 |
| chr8.1 | exon | 23004545 | 23004550 | 23004545 | ID=MS.gene73015.t1.exon5;Parent=MS.gene73015.t1 |
| chr8.1 | CDS | 23004545 | 23004550 | 23004545 | ID=cds.MS.gene73015.t1;Parent=MS.gene73015.t1 |
| Gene Sequence |
| Protein sequence |
