AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73889


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73889.t1 MTR_3g082210 85.437 103 15 0 1 103 1 103 2.88e-53 162
MS.gene73889.t1 MTR_3g082250 69.444 108 24 3 1 103 12 115 1.02e-42 135
MS.gene73889.t1 MTR_1g041690 60.185 108 36 3 1 103 1 106 5.70e-35 115
MS.gene73889.t1 MTR_3g082260 58.333 108 38 3 1 103 1 106 3.61e-33 111
MS.gene73889.t1 MTR_5g007070 70.732 82 12 1 1 70 15 96 1.30e-31 107
MS.gene73889.t1 MTR_5g084660 43.200 125 47 5 1 102 10 133 6.57e-18 73.6
MS.gene73889.t1 MTR_0635s0010 50.769 65 32 0 1 65 9 73 1.05e-16 68.6
MS.gene73889.t1 MTR_2g027600 50.769 65 32 0 1 65 11 75 1.10e-16 68.9
MS.gene73889.t1 MTR_6g082190 47.170 53 28 0 1 53 1 53 5.10e-12 59.7
MS.gene73889.t1 MTR_6g082330 43.750 64 36 0 1 64 11 74 4.61e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73889.t1 AT3G20180 40.000 85 50 1 1 85 7 90 3.53e-14 63.5
MS.gene73889.t1 AT3G20180 40.000 85 50 1 1 85 9 92 3.90e-14 63.5
MS.gene73889.t1 AT3G20180 40.000 85 50 1 1 85 11 94 4.49e-14 63.5
MS.gene73889.t1 AT3G20180 44.737 76 40 1 1 74 30 105 5.32e-14 63.5
MS.gene73889.t1 AT5G48290 48.649 74 35 1 1 71 1 74 7.15e-14 63.9
MS.gene73889.t1 AT5G48290 48.649 74 35 1 1 71 11 84 7.90e-14 63.9
MS.gene73889.t1 AT3G07600 44.928 69 38 0 1 69 11 79 1.80e-13 62.8

Find 0 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGAAAGAAGAACCAATTA+TGG - 24212:3068-3087 MS.gene73889:intron 25.0%
! AAGGATCAAATAGAAGTAAT+CGG - 24212:2949-2968 MS.gene73889:CDS 25.0%
! AATCATGTCCAAAAATATTC+AGG - 24212:2895-2914 MS.gene73889:CDS 25.0%
! TAGTTTGGATAATTAAGCTA+TGG + 24212:2876-2895 MS.gene73889:intergenic 25.0%
!!! ATTTTTGGACATGATTAGTT+TGG + 24212:2891-2910 MS.gene73889:intergenic 25.0%
! TTTCCACTCCTGAATATTTT+TGG + 24212:2906-2925 MS.gene73889:intergenic 30.0%
AAGAAGAACCAATTATGGCA+TGG - 24212:3073-3092 MS.gene73889:intron 35.0%
GAAATTCTGTCATGCAGAAT+TGG - 24212:3014-3033 MS.gene73889:intron 35.0%
TGGTGAAGTGAAAAAGAAAG+AGG - 24212:3044-3063 MS.gene73889:intron 35.0%
TGTCCAAAAATATTCAGGAG+TGG - 24212:2900-2919 MS.gene73889:CDS 35.0%
!! TAGCAGTTTTGTGAGTCTAA+CGG + 24212:2989-3008 MS.gene73889:intergenic 35.0%
AAAGCCATGAAGATTGCAGT+TGG - 24212:2782-2801 MS.gene73889:CDS 40.0%
AACTCCAACTGCAATCTTCA+TGG + 24212:2789-2808 MS.gene73889:intergenic 40.0%
AACTGCAATCTTCATGGCTT+TGG + 24212:2783-2802 MS.gene73889:intergenic 40.0%
AAGATTGCAGTTGGAGTTTC+AGG - 24212:2791-2810 MS.gene73889:CDS 40.0%
AGCAATACAAGGAGATAGCA+AGG - 24212:2930-2949 MS.gene73889:CDS 40.0%
TGAGGATAACCAGTTACACA+AGG + 24212:3099-3118 MS.gene73889:intergenic 40.0%
!!! TGGCTTTGGACCTTGATTTT+TGG + 24212:2769-2788 MS.gene73889:intergenic 40.0%
ACACAAGGCCATGCCATAAT+TGG + 24212:3084-3103 MS.gene73889:intergenic 45.0%
CATGCAGAATTGGTGAGTGT+TGG - 24212:3024-3043 MS.gene73889:intron 45.0%
TGTCCCTGTGTGTGAGATAA+GGG - 24212:3125-3144 MS.gene73889:CDS 45.0%
TTATCTCACACACAGGGACA+GGG + 24212:3125-3144 MS.gene73889:intergenic 45.0%
! GTGGAAAGTGCAGCAATACA+AGG - 24212:2919-2938 MS.gene73889:CDS 45.0%
!! TTTGAATTAAAAATAATTAA+TGG + 24212:2841-2860 MS.gene73889:intergenic 5.0%
CGTCCCTTATCTCACACACA+GGG + 24212:3131-3150 MS.gene73889:intergenic 50.0%
CTGTCCCTGTGTGTGAGATA+AGG - 24212:3124-3143 MS.gene73889:CDS 50.0%
CTTATCTCACACACAGGGAC+AGG + 24212:3126-3145 MS.gene73889:intergenic 50.0%
TCGTCCCTTATCTCACACAC+AGG + 24212:3132-3151 MS.gene73889:intergenic 50.0%
!! ATGGCATGGCCTTGTGTAAC+TGG - 24212:3087-3106 MS.gene73889:intron 50.0%
CACACAGGGACAGGGTAATG+AGG + 24212:3117-3136 MS.gene73889:intergenic 55.0%


Chromosome Type Strat End Strand Name
24212 gene 2758 3173 2758 ID=MS.gene73889
24212 mRNA 2758 3173 2758 ID=MS.gene73889.t1;Parent=MS.gene73889
24212 exon 3119 3173 3119 ID=MS.gene73889.t1.exon1;Parent=MS.gene73889.t1
24212 CDS 3119 3173 3119 ID=cds.MS.gene73889.t1;Parent=MS.gene73889.t1
24212 exon 2758 3014 2758 ID=MS.gene73889.t1.exon2;Parent=MS.gene73889.t1
24212 CDS 2758 3014 2758 ID=cds.MS.gene73889.t1;Parent=MS.gene73889.t1
Gene Sequence

>MS.gene73889

ATGCACAGCCAAAAATCAAGGTCCAAAGCCATGAAGATTGCAGTTGGAGTTTCAGGTAATAAAATTCACTGATTTTGTCACCATTAATTATTTTTAATTCAAAATTTGTTCATAACCATAGCTTAATTATCCAAACTAATCATGTCCAAAAATATTCAGGAGTGGAAAGTGCAGCAATACAAGGAGATAGCAAGGATCAAATAGAAGTAATCGGAGAACAAATAGACTCCGTTAGACTCACAAAACTGCTAAGAAAGAAATTCTGTCATGCAGAATTGGTGAGTGTTGGTGAAGTGAAAAAGAAAGAGGAAAAGAAAGAAGAACCAATTATGGCATGGCCTTGTGTAACTGGTTATCCTCATTACCCTGTCCCTGTGTGTGAGATAAGGGACGACTCTTCTTGTTCCATCATGTGA

Protein sequence

>MS.gene73889.t1

MHSQKSRSKAMKIAVGVSGVESAAIQGDSKDQIEVIGEQIDSVRLTKLLRKKFCHAELVSVGEVKKKEEKKEEPIMAWPCVTGYPHYPVPVCEIRDDSSCSIM