AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74447

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74447.t1 MTR_6g023990 93.511 262 16 1 1 261 5 266 0.0 501
MS.gene74447.t1 MTR_6g023590 85.000 260 39 0 2 261 6 265 1.85e-164 456
MS.gene74447.t1 MTR_6g023600 85.769 260 37 0 2 261 6 265 5.63e-157 437
MS.gene74447.t1 MTR_6g023950 85.547 256 37 0 2 257 6 261 1.29e-154 431
MS.gene74447.t1 MTR_6g023600 83.846 260 36 1 2 261 6 259 4.32e-150 419
MS.gene74447.t1 MTR_6g023610 72.308 260 72 0 2 261 6 265 6.62e-140 394
MS.gene74447.t1 MTR_6g023910 72.868 258 70 0 4 261 8 265 2.56e-136 385
MS.gene74447.t1 MTR_7g110130 50.195 257 124 2 1 257 1 253 6.15e-83 249
MS.gene74447.t1 MTR_7g110140 47.826 253 130 2 5 257 2 252 4.34e-78 237
MS.gene74447.t1 MTR_2g090165 46.640 253 133 2 5 257 2 252 1.34e-77 236
MS.gene74447.t1 MTR_7g105460 48.400 250 127 2 8 257 12 259 3.64e-76 232
MS.gene74447.t1 MTR_4g052340 47.177 248 126 3 8 255 13 255 5.51e-73 224
MS.gene74447.t1 MTR_4g052300 47.036 253 129 3 4 256 3 250 5.68e-73 224
MS.gene74447.t1 MTR_4g052350 47.036 253 129 3 8 260 13 260 1.58e-72 223
MS.gene74447.t1 MTR_4g052320 46.063 254 133 3 4 257 3 252 3.53e-71 219
MS.gene74447.t1 MTR_7g110160 45.059 253 119 2 5 257 2 234 5.33e-69 213
MS.gene74447.t1 MTR_6g035395 48.000 250 115 3 8 257 4 238 2.43e-68 211
MS.gene74447.t1 MTR_7g071680 43.478 276 134 5 1 257 21 293 3.28e-68 213
MS.gene74447.t1 MTR_4g078630 44.747 257 131 3 8 257 18 270 1.82e-63 200
MS.gene74447.t1 MTR_7g107670 39.850 266 152 4 1 261 25 287 2.73e-61 195
MS.gene74447.t1 MTR_7g083870 42.578 256 138 6 8 257 34 286 9.78e-61 194
MS.gene74447.t1 MTR_3g020670 41.445 263 133 6 8 257 15 269 9.39e-55 178
MS.gene74447.t1 MTR_6g023940 47.143 210 41 3 4 209 8 151 5.31e-52 167
MS.gene74447.t1 MTR_7g105590 44.974 189 102 2 8 196 7 193 1.56e-51 167
MS.gene74447.t1 MTR_7g105580 37.200 250 137 3 8 257 7 236 1.43e-49 164
MS.gene74447.t1 MTR_4g084800 51.799 139 66 1 8 146 12 149 6.65e-47 154
MS.gene74447.t1 MTR_7g105570 45.000 180 98 1 78 257 38 216 1.09e-43 148
MS.gene74447.t1 MTR_3g113870 42.077 183 103 3 17 199 29 208 9.32e-43 145
MS.gene74447.t1 MTR_7g113650 32.955 264 152 8 8 258 39 290 1.42e-30 115
MS.gene74447.t1 MTR_1g082760 33.708 267 152 9 5 258 36 290 1.44e-30 115
MS.gene74447.t1 MTR_4g131740 30.741 270 159 8 4 258 35 291 1.15e-26 105
MS.gene74447.t1 MTR_4g132850 31.621 253 160 6 8 257 6 248 1.85e-26 103
MS.gene74447.t1 MTR_7g094510 31.418 261 165 6 5 258 2 255 1.33e-25 101
MS.gene74447.t1 MTR_3g085740 28.846 260 162 8 8 258 74 319 2.45e-25 102
MS.gene74447.t1 MTR_7g094520 31.679 262 161 8 6 258 8 260 4.05e-22 92.4
MS.gene74447.t1 MTR_1196s0010 33.597 253 147 9 12 258 277 514 5.00e-21 92.4
MS.gene74447.t1 MTR_4g064887 27.799 259 177 6 2 257 8 259 2.53e-19 85.1
MS.gene74447.t1 MTR_7g063320 28.846 260 177 6 3 258 9 264 1.32e-17 80.5
MS.gene74447.t1 MTR_7g063360 27.376 263 175 7 3 257 10 264 2.76e-16 76.6
MS.gene74447.t1 MTR_4g058810 25.591 254 180 6 9 256 11 261 2.79e-16 76.6
MS.gene74447.t1 MTR_4g058840 25.869 259 173 7 9 256 11 261 1.08e-15 75.1
MS.gene74447.t1 MTR_4g058820 25.869 259 173 7 9 256 11 261 1.08e-15 75.1
MS.gene74447.t1 MTR_1g068955 28.906 256 171 5 6 255 11 261 1.41e-15 75.1
MS.gene74447.t1 MTR_7g063330 26.641 259 181 7 3 257 9 262 1.48e-15 74.7
MS.gene74447.t1 MTR_3g068065 28.016 257 165 10 9 257 17 261 8.73e-15 72.8
MS.gene74447.t1 MTR_3g068155 28.405 257 171 9 9 260 19 267 1.40e-14 72.0
MS.gene74447.t1 MTR_7g105560 62.500 56 20 1 8 63 12 66 2.53e-14 68.9
MS.gene74447.t1 MTR_3g068085 27.843 255 168 9 9 257 15 259 6.15e-14 70.1
MS.gene74447.t1 MTR_1g051165 24.335 263 179 7 8 256 13 269 6.24e-14 70.5
MS.gene74447.t1 MTR_3g068175 27.239 268 161 9 5 257 13 261 1.32e-13 69.3
MS.gene74447.t1 MTR_4g086400 28.365 208 129 6 10 202 5 207 1.99e-13 70.1
MS.gene74447.t1 MTR_4g064887 26.667 210 129 5 2 199 8 204 1.06e-12 65.9
MS.gene74447.t1 MTR_3g068175 26.838 272 161 10 5 257 13 265 1.50e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74447.t1 AT2G47140 49.012 253 127 2 5 257 2 252 1.79e-80 243
MS.gene74447.t1 AT3G29260 49.609 256 120 5 5 257 2 251 2.43e-73 225
MS.gene74447.t1 AT3G29250 47.431 253 131 2 5 257 2 252 3.87e-73 224
MS.gene74447.t1 AT3G29250 47.431 253 131 2 5 257 38 288 8.81e-73 224
MS.gene74447.t1 AT2G47120 45.736 258 126 5 5 257 2 250 1.39e-71 220
MS.gene74447.t1 AT2G47130 47.266 256 126 4 5 257 2 251 2.22e-71 219
MS.gene74447.t1 AT3G29250 47.059 255 131 3 5 257 38 290 1.10e-70 219
MS.gene74447.t1 AT2G47120 45.490 255 125 5 8 257 6 251 2.06e-70 217
MS.gene74447.t1 AT3G26770 44.574 258 134 5 6 257 38 292 9.97e-67 209
MS.gene74447.t1 AT3G26760 40.840 262 144 5 4 257 31 289 5.99e-61 194
MS.gene74447.t1 AT4G03140 41.176 255 142 4 8 257 77 328 1.01e-59 192
MS.gene74447.t1 AT3G51680 40.530 264 141 4 8 256 31 293 3.94e-56 182
MS.gene74447.t1 AT1G52340 42.912 261 135 6 8 257 17 274 4.90e-56 181
MS.gene74447.t1 AT3G42960 42.586 263 137 7 4 257 3 260 4.65e-52 171
MS.gene74447.t1 AT2G47150 33.858 254 103 8 9 257 1 194 8.02e-30 111
MS.gene74447.t1 AT1G54870 32.443 262 160 8 6 258 81 334 1.04e-27 108
MS.gene74447.t1 AT1G54870 32.443 262 160 8 6 258 79 332 1.05e-27 108
MS.gene74447.t1 AT3G05260 30.303 264 166 8 4 258 32 286 4.28e-25 101
MS.gene74447.t1 AT5G18210 30.651 261 162 7 9 258 8 260 5.64e-25 100
MS.gene74447.t1 AT4G13180 28.309 272 161 9 4 258 8 262 4.59e-23 95.1
MS.gene74447.t1 AT4G05530 31.395 258 168 5 1 257 1 250 3.54e-22 92.4
MS.gene74447.t1 AT3G03980 29.242 277 170 7 1 258 1 270 1.11e-21 91.7
MS.gene74447.t1 AT5G18210 31.148 244 149 7 9 241 8 243 1.43e-21 91.3
MS.gene74447.t1 AT3G04000 28.253 269 163 7 7 256 12 269 7.64e-20 86.7
MS.gene74447.t1 AT2G29340 29.779 272 155 10 1 257 1 251 9.50e-20 86.3
MS.gene74447.t1 AT2G29340 29.779 272 155 10 1 257 1 251 1.53e-19 86.3
MS.gene74447.t1 AT1G24360 27.626 257 167 8 8 257 73 317 1.73e-19 86.3
MS.gene74447.t1 AT2G29310 29.259 270 145 10 9 257 7 251 1.68e-18 82.8
MS.gene74447.t1 AT5G06060 27.675 271 164 8 1 257 1 253 2.01e-17 79.7
MS.gene74447.t1 AT2G29350 28.996 269 160 10 1 256 7 257 2.10e-17 79.7
MS.gene74447.t1 AT1G63380 26.562 256 176 7 9 256 15 266 3.85e-17 79.3
MS.gene74447.t1 AT1G63380 26.562 256 176 7 9 256 19 270 3.97e-17 79.3
MS.gene74447.t1 AT1G63380 26.562 256 176 7 9 256 17 268 4.07e-17 79.3
MS.gene74447.t1 AT1G63380 26.562 256 176 7 9 256 22 273 4.73e-17 79.3
MS.gene74447.t1 AT2G29330 27.273 264 158 8 9 257 7 251 6.49e-17 78.6
MS.gene74447.t1 AT2G30670 26.336 262 163 8 9 257 7 251 7.99e-17 78.2
MS.gene74447.t1 AT1G07450 29.963 267 146 11 9 257 8 251 9.04e-17 78.2
MS.gene74447.t1 AT1G62610 26.172 256 177 7 9 256 13 264 1.20e-16 77.8
MS.gene74447.t1 AT1G62610 26.172 256 177 7 9 256 14 265 1.23e-16 77.8
MS.gene74447.t1 AT1G62610 26.172 256 177 7 9 256 15 266 1.26e-16 77.8
MS.gene74447.t1 AT1G62610 26.172 256 177 7 9 256 19 270 1.37e-16 77.8
MS.gene74447.t1 AT2G29370 28.058 278 156 11 3 257 4 260 1.80e-16 77.4
MS.gene74447.t1 AT2G29360 30.739 257 158 10 9 257 16 260 3.28e-16 76.6
MS.gene74447.t1 AT2G29290 28.571 266 152 9 9 257 7 251 4.04e-15 73.6
MS.gene74447.t1 AT3G46170 24.904 261 175 8 8 256 25 276 6.42e-15 73.2
MS.gene74447.t1 AT3G55290 24.904 261 175 7 8 256 16 267 1.33e-14 72.4
MS.gene74447.t1 AT3G55290 24.904 261 175 7 8 256 17 268 1.61e-14 72.0
MS.gene74447.t1 AT1G07440 26.336 262 174 7 3 257 6 255 2.17e-14 71.6
MS.gene74447.t1 AT2G29300 28.571 273 148 13 9 259 7 254 7.95e-14 70.1
MS.gene74447.t1 AT2G29310 29.545 220 119 8 49 257 55 249 8.62e-14 69.7
MS.gene74447.t1 AT2G29340 28.505 214 122 8 1 200 1 197 1.10e-13 68.6
MS.gene74447.t1 AT2G29340 28.505 214 122 8 1 200 1 197 1.10e-13 68.6
MS.gene74447.t1 AT2G29300 28.727 275 148 13 9 260 7 256 1.65e-13 68.9
MS.gene74447.t1 AT3G55310 24.715 263 173 8 8 256 16 267 6.27e-13 67.4
MS.gene74447.t1 AT2G29350 28.638 213 125 8 1 200 7 205 1.81e-12 65.5
MS.gene74447.t1 AT3G47360 26.289 194 131 5 12 200 48 234 3.55e-12 65.5
MS.gene74447.t1 AT3G47350 23.695 249 165 8 9 241 44 283 4.27e-12 65.5
MS.gene74447.t1 AT2G29310 29.384 211 110 8 9 200 7 197 7.52e-12 63.2
MS.gene74447.t1 AT2G29350 28.436 211 130 7 49 256 27 219 1.96e-11 62.8
MS.gene74447.t1 AT5G50770 29.412 187 124 5 9 192 45 226 2.15e-11 63.5
MS.gene74447.t1 AT3G47350 24.365 197 137 5 9 200 44 233 2.88e-11 63.2

Find 71 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGACGGTGGATTTGTTTA+TGG 0.203718 6.4:-133843 MS.gene74447:CDS
TAATCTTAGACTAGAGTTTC+TGG 0.220802 6.4:+134719 None:intergenic
CAATGGTGGAGGGGCGCGTT+AGG 0.251861 6.4:-134199 MS.gene74447:CDS
CAATCAACGGTTAATACTTA+TGG 0.256946 6.4:-134489 MS.gene74447:CDS
GCTGACATCCAAGATGAATT+TGG 0.261535 6.4:-134600 MS.gene74447:CDS
ATGTCAAAGCACGCAGTATT+GGG 0.309736 6.4:-134110 MS.gene74447:CDS
AACTCTAGTCTAAGATTATC+AGG 0.316109 6.4:-134714 MS.gene74447:CDS
GCGTGCAGCCAGCGTGCAAC+TGG 0.336790 6.4:-134081 MS.gene74447:CDS
AATGGTGGAGGGGCGCGTTA+GGG 0.343850 6.4:-134198 MS.gene74447:CDS
ATGGTGGAGGGGCGCGTTAG+GGG 0.370385 6.4:-134197 MS.gene74447:CDS
ATCGGTTGGACTAAAGATTC+CGG 0.377217 6.4:+134316 None:intergenic
CTTGAATGCCAAATTCATCT+TGG 0.396027 6.4:+134592 None:intergenic
GATGTCAAAGCACGCAGTAT+TGG 0.423772 6.4:-134111 MS.gene74447:CDS
AGTGGATGTAGGTGCACCTT+TGG 0.435399 6.4:+134550 None:intergenic
GAACATTGATTGTGAATAAA+CGG 0.441133 6.4:+134258 None:intergenic
CGCGCCCCTCCACCATTGCA+CGG 0.441858 6.4:+134204 None:intergenic
TGGTGGAGGGGCGCGTTAGG+GGG 0.443091 6.4:-134196 MS.gene74447:CDS
TGGACTAAAGATTCCGGCAT+TGG 0.443211 6.4:+134322 None:intergenic
AAAGGATATGCAAGACTTGA+AGG 0.443745 6.4:-133954 MS.gene74447:CDS
TGCAGCCCGTGCAATGGTGG+AGG 0.459063 6.4:-134210 MS.gene74447:CDS
AAGGCACAAGAGACTTATAA+AGG 0.461345 6.4:-133972 MS.gene74447:CDS
TCCAACACAGTTTGATCGGT+TGG 0.467057 6.4:+134302 None:intergenic
ACAGCATCTGCCACATGCTT+AGG 0.471667 6.4:+133918 None:intergenic
TTATTCACAATCAATGTTCG+AGG 0.474144 6.4:-134254 MS.gene74447:CDS
GATATAATGTTCACCAATGC+CGG 0.479834 6.4:-134335 MS.gene74447:CDS
GTGTGCACGGGGAGTGTTCA+CGG 0.484890 6.4:-134167 MS.gene74447:CDS
TGATTGTGAATAAACGGTCT+AGG 0.499386 6.4:+134264 None:intergenic
AAAGGTGTCGCGAGTCCATT+CGG 0.500418 6.4:+134026 None:intergenic
ACTAGCACCACCCGTGATGA+TGG 0.502192 6.4:+134683 None:intergenic
CACAATCAATGTTCGAGGAA+TGG 0.504145 6.4:-134249 MS.gene74447:CDS
ACGGGTGGTGCTAGTGGCAT+CGG 0.517674 6.4:-134675 MS.gene74447:CDS
GCAGCCCGTGCAATGGTGGA+GGG 0.522760 6.4:-134209 MS.gene74447:CDS
GGCAAAATAGCCATCATCAC+GGG 0.526330 6.4:-134693 MS.gene74447:CDS
CACCGTCCACACTAAGATCA+AGG 0.530151 6.4:+133856 None:intergenic
AATGATGCTGAGTTTGTCAC+TGG 0.531303 6.4:-133879 MS.gene74447:CDS
AACGGTTAATACTTATGGAC+AGG 0.532873 6.4:-134484 MS.gene74447:intron
TTTAGGGGTGAGTGAGGAGA+AGG 0.535551 6.4:-133991 MS.gene74447:CDS
GCGCCCCTCCACCATTGCAC+GGG 0.541845 6.4:+134205 None:intergenic
GAGTTCCAACACAGTTTGAT+CGG 0.542405 6.4:+134298 None:intergenic
GGGAGTGTTCACGGTAGTCA+TGG 0.546162 6.4:-134158 MS.gene74447:CDS
ATCATCACGGGTGGTGCTAG+TGG 0.552992 6.4:-134681 MS.gene74447:CDS
TGTGAATAAACGGTCTAGGT+TGG 0.553958 6.4:+134268 None:intergenic
GTGAACTGTGTCTCGCCGAA+TGG 0.562393 6.4:-134041 MS.gene74447:CDS
GGCCTTGATCTTAGTGTGGA+CGG 0.567765 6.4:-133858 MS.gene74447:CDS
AGCGTGCAACTGGCCGCACA+CGG 0.572901 6.4:-134071 MS.gene74447:CDS
TAGTGGCATCGGTGAAGCCA+CGG 0.573146 6.4:-134664 MS.gene74447:CDS
CAGCCCGTGCAATGGTGGAG+GGG 0.580685 6.4:-134208 MS.gene74447:CDS
GTCTTTGCTAACGAAGGCGT+CGG 0.585405 6.4:-134636 MS.gene74447:CDS
TCCAACCGATCAAACTGTGT+TGG 0.590298 6.4:-134303 MS.gene74447:CDS
ACATGCAGCCCGTGCAATGG+TGG 0.591192 6.4:-134213 MS.gene74447:CDS
TCATGGATTCTTGAGGTCAA+CGG 0.591437 6.4:-134141 MS.gene74447:CDS
ATTCAAGTAGCTGCGTCCAT+CGG 0.592404 6.4:-134576 MS.gene74447:CDS
GGTGCACCTTTGGTTGCCGA+TGG 0.593029 6.4:+134560 None:intergenic
TGTCAAAGCACGCAGTATTG+GGG 0.601212 6.4:-134109 MS.gene74447:CDS
CTGCGTCCATCGGCAACCAA+AGG 0.606542 6.4:-134566 MS.gene74447:CDS
CACTGGCCTTGATCTTAGTG+TGG 0.608245 6.4:-133862 MS.gene74447:CDS
GTGTGCGGCCAGTTGCACGC+TGG 0.617516 6.4:+134073 None:intergenic
GTTCACTCTTATTCCGTGTG+CGG 0.626381 6.4:+134058 None:intergenic
GAAACATGCAGCCCGTGCAA+TGG 0.638047 6.4:-134216 MS.gene74447:CDS
AGGCAAAATAGCCATCATCA+CGG 0.640770 6.4:-134694 MS.gene74447:CDS
GGATGTCAGCAATCACAACA+TGG 0.642460 6.4:+134613 None:intergenic
TGCCACATCACAGTGGATGT+AGG 0.644985 6.4:+134539 None:intergenic
CACCTACATCCACTGTGATG+TGG 0.646592 6.4:-134541 MS.gene74447:CDS
GCGCGTGTCTTTGCTAACGA+AGG 0.654619 6.4:-134642 MS.gene74447:CDS
ATATGCAAGACTTGAAGGAG+TGG 0.664008 6.4:-133949 MS.gene74447:CDS
ACGGTAGTCATGGATTCTTG+AGG 0.671995 6.4:-134148 MS.gene74447:CDS
GGTGCTCACACCTAAGCATG+TGG 0.673012 6.4:-133928 MS.gene74447:CDS
AAAATAGCCATCATCACGGG+TGG 0.677019 6.4:-134690 MS.gene74447:CDS
CTTCGTCTGCCACATCACAG+TGG 0.713218 6.4:+134532 None:intergenic
GTTAGCAAAGACACGCGCCG+TGG 0.733448 6.4:+134647 None:intergenic
CTTGATCTTAGTGTGGACGG+TGG 0.733763 6.4:-133855 MS.gene74447:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGACTTGTAAATTTTTA+GGG - chr6.4:134552-134571 MS.gene74447:CDS 20.0%
!!! TTTGACTTGTAAATTTTTAG+GGG - chr6.4:134553-134572 MS.gene74447:CDS 20.0%
! CCTAAAAATTTACAAGTCAA+AGG + chr6.4:134554-134573 None:intergenic 25.0%
!! GAACATTGATTGTGAATAAA+CGG + chr6.4:134304-134323 None:intergenic 25.0%
!!! CCTTTGACTTGTAAATTTTT+AGG - chr6.4:134551-134570 MS.gene74447:CDS 25.0%
CAAAAATCTCGTTCAATCAA+CGG - chr6.4:134057-134076 MS.gene74447:CDS 30.0%
CAATCAACGGTTAATACTTA+TGG - chr6.4:134070-134089 MS.gene74447:CDS 30.0%
TAATCTTAGACTAGAGTTTC+TGG + chr6.4:133843-133862 None:intergenic 30.0%
TTATTCACAATCAATGTTCG+AGG - chr6.4:134305-134324 MS.gene74447:CDS 30.0%
! AACTCTAGTCTAAGATTATC+AGG - chr6.4:133845-133864 MS.gene74447:CDS 30.0%
! ATTTCCTATCTTAGCTTTCA+AGG - chr6.4:134105-134124 MS.gene74447:CDS 30.0%
! TTTCCTATCTTAGCTTTCAA+GGG - chr6.4:134106-134125 MS.gene74447:CDS 30.0%
AAAGGATATGCAAGACTTGA+AGG - chr6.4:134605-134624 MS.gene74447:CDS 35.0%
AACGGTTAATACTTATGGAC+AGG - chr6.4:134075-134094 MS.gene74447:CDS 35.0%
AAGGCACAAGAGACTTATAA+AGG - chr6.4:134587-134606 MS.gene74447:CDS 35.0%
CTTGAATGCCAAATTCATCT+TGG + chr6.4:133970-133989 None:intergenic 35.0%
GATATAATGTTCACCAATGC+CGG - chr6.4:134224-134243 MS.gene74447:CDS 35.0%
GATTATGTTATTCTCACACC+AGG - chr6.4:134199-134218 MS.gene74447:CDS 35.0%
TGATTGTGAATAAACGGTCT+AGG + chr6.4:134298-134317 None:intergenic 35.0%
TGGTGAACATTATATCTACC+TGG + chr6.4:134220-134239 None:intergenic 35.0%
!! TAAATTTTTAGGGGTGAGTG+AGG - chr6.4:134562-134581 MS.gene74447:CDS 35.0%
AATGATGCTGAGTTTGTCAC+TGG - chr6.4:134680-134699 MS.gene74447:CDS 40.0%
ACACCCTTGAAAGCTAAGAT+AGG + chr6.4:134112-134131 None:intergenic 40.0%
AGGCAAAATAGCCATCATCA+CGG - chr6.4:133865-133884 MS.gene74447:CDS 40.0%
ATATGCAAGACTTGAAGGAG+TGG - chr6.4:134610-134629 MS.gene74447:CDS 40.0%
ATCGGTTGGACTAAAGATTC+CGG + chr6.4:134246-134265 None:intergenic 40.0%
ATGTCAAAGCACGCAGTATT+GGG - chr6.4:134449-134468 MS.gene74447:intron 40.0%
CACAATCAATGTTCGAGGAA+TGG - chr6.4:134310-134329 MS.gene74447:CDS 40.0%
GAGTTCCAACACAGTTTGAT+CGG + chr6.4:134264-134283 None:intergenic 40.0%
GCTGACATCCAAGATGAATT+TGG - chr6.4:133959-133978 MS.gene74447:CDS 40.0%
TCATGGATTCTTGAGGTCAA+CGG - chr6.4:134418-134437 MS.gene74447:intron 40.0%
TGTGAATAAACGGTCTAGGT+TGG + chr6.4:134294-134313 None:intergenic 40.0%
AAAATAGCCATCATCACGGG+TGG - chr6.4:133869-133888 MS.gene74447:CDS 45.0%
ACGGTAGTCATGGATTCTTG+AGG - chr6.4:134411-134430 MS.gene74447:intron 45.0%
ATTCAAGTAGCTGCGTCCAT+CGG - chr6.4:133983-134002 MS.gene74447:CDS 45.0%
GATGTCAAAGCACGCAGTAT+TGG - chr6.4:134448-134467 MS.gene74447:intron 45.0%
GGATGTCAGCAATCACAACA+TGG + chr6.4:133949-133968 None:intergenic 45.0%
GGCAAAATAGCCATCATCAC+GGG - chr6.4:133866-133885 MS.gene74447:CDS 45.0%
GTTCACTCTTATTCCGTGTG+CGG + chr6.4:134504-134523 None:intergenic 45.0%
TCCAACACAGTTTGATCGGT+TGG + chr6.4:134260-134279 None:intergenic 45.0%
TCCAACCGATCAAACTGTGT+TGG - chr6.4:134256-134275 MS.gene74447:CDS 45.0%
TGGACTAAAGATTCCGGCAT+TGG + chr6.4:134240-134259 None:intergenic 45.0%
TGTCAAAGCACGCAGTATTG+GGG - chr6.4:134450-134469 MS.gene74447:intron 45.0%
! GTGGACGGTGGATTTGTTTA+TGG - chr6.4:134716-134735 MS.gene74447:CDS 45.0%
ACAGCATCTGCCACATGCTT+AGG + chr6.4:134644-134663 None:intergenic 50.0%
AGTGGATGTAGGTGCACCTT+TGG + chr6.4:134012-134031 None:intergenic 50.0%
CACCGTCCACACTAAGATCA+AGG + chr6.4:134706-134725 None:intergenic 50.0%
CACCTACATCCACTGTGATG+TGG - chr6.4:134018-134037 MS.gene74447:CDS 50.0%
CTTGATCTTAGTGTGGACGG+TGG - chr6.4:134704-134723 MS.gene74447:CDS 50.0%
GGCCTTGATCTTAGTGTGGA+CGG - chr6.4:134701-134720 MS.gene74447:CDS 50.0%
TGCCACATCACAGTGGATGT+AGG + chr6.4:134023-134042 None:intergenic 50.0%
TTTAGGGGTGAGTGAGGAGA+AGG - chr6.4:134568-134587 MS.gene74447:CDS 50.0%
! GTCTTTGCTAACGAAGGCGT+CGG - chr6.4:133923-133942 MS.gene74447:CDS 50.0%
!! AAAGGTGTCGCGAGTCCATT+CGG + chr6.4:134536-134555 None:intergenic 50.0%
!! CACTGGCCTTGATCTTAGTG+TGG - chr6.4:134697-134716 MS.gene74447:CDS 50.0%
!! TAGGGGGAGTATTGTGTGCA+CGG - chr6.4:134379-134398 MS.gene74447:intron 50.0%
ACTAGCACCACCCGTGATGA+TGG + chr6.4:133879-133898 None:intergenic 55.0%
CTTCGTCTGCCACATCACAG+TGG + chr6.4:134030-134049 None:intergenic 55.0%
GAAACATGCAGCCCGTGCAA+TGG - chr6.4:134343-134362 MS.gene74447:intron 55.0%
GCGCGTGTCTTTGCTAACGA+AGG - chr6.4:133917-133936 MS.gene74447:CDS 55.0%
GGTGCTCACACCTAAGCATG+TGG - chr6.4:134631-134650 MS.gene74447:CDS 55.0%
GTGAACTGTGTCTCGCCGAA+TGG - chr6.4:134518-134537 MS.gene74447:CDS 55.0%
! ATCATCACGGGTGGTGCTAG+TGG - chr6.4:133878-133897 MS.gene74447:CDS 55.0%
!! AGGGGGAGTATTGTGTGCAC+GGG - chr6.4:134380-134399 MS.gene74447:intron 55.0%
!! GGGAGTGTTCACGGTAGTCA+TGG - chr6.4:134401-134420 MS.gene74447:intron 55.0%
!! TAGTGGCATCGGTGAAGCCA+CGG - chr6.4:133895-133914 MS.gene74447:CDS 55.0%
ACATGCAGCCCGTGCAATGG+TGG - chr6.4:134346-134365 MS.gene74447:intron 60.0%
CTGCGTCCATCGGCAACCAA+AGG - chr6.4:133993-134012 MS.gene74447:CDS 60.0%
GGTGCACCTTTGGTTGCCGA+TGG + chr6.4:134002-134021 None:intergenic 60.0%
GTTAGCAAAGACACGCGCCG+TGG + chr6.4:133915-133934 None:intergenic 60.0%
! AATGGTGGAGGGGCGCGTTA+GGG - chr6.4:134361-134380 MS.gene74447:intron 60.0%
! ACGGGTGGTGCTAGTGGCAT+CGG - chr6.4:133884-133903 MS.gene74447:CDS 60.0%
!! GGGGGAGTATTGTGTGCACG+GGG - chr6.4:134381-134400 MS.gene74447:intron 60.0%
!! GTGTGCACGGGGAGTGTTCA+CGG - chr6.4:134392-134411 MS.gene74447:intron 60.0%
AGCGTGCAACTGGCCGCACA+CGG - chr6.4:134488-134507 MS.gene74447:CDS 65.0%
TGCAGCCCGTGCAATGGTGG+AGG - chr6.4:134349-134368 MS.gene74447:intron 65.0%
! ATGGTGGAGGGGCGCGTTAG+GGG - chr6.4:134362-134381 MS.gene74447:intron 65.0%
! CAATGGTGGAGGGGCGCGTT+AGG - chr6.4:134360-134379 MS.gene74447:intron 65.0%
! CAGCCCGTGCAATGGTGGAG+GGG - chr6.4:134351-134370 MS.gene74447:intron 65.0%
! GCAGCCCGTGCAATGGTGGA+GGG - chr6.4:134350-134369 MS.gene74447:intron 65.0%
CGCGCCCCTCCACCATTGCA+CGG + chr6.4:134358-134377 None:intergenic 70.0%
GCGCCCCTCCACCATTGCAC+GGG + chr6.4:134357-134376 None:intergenic 70.0%
GCGTGCAGCCAGCGTGCAAC+TGG - chr6.4:134478-134497 MS.gene74447:intron 70.0%
GTGTGCGGCCAGTTGCACGC+TGG + chr6.4:134489-134508 None:intergenic 70.0%
! TGGTGGAGGGGCGCGTTAGG+GGG - chr6.4:134363-134382 MS.gene74447:intron 70.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 133836 134745 133836 ID=MS.gene74447
chr6.4 mRNA 133836 134745 133836 ID=MS.gene74447.t1;Parent=MS.gene74447
chr6.4 exon 134485 134745 134485 ID=MS.gene74447.t1.exon1;Parent=MS.gene74447.t1
chr6.4 CDS 134485 134745 134485 ID=cds.MS.gene74447.t1;Parent=MS.gene74447.t1
chr6.4 exon 133836 134360 133836 ID=MS.gene74447.t1.exon2;Parent=MS.gene74447.t1
chr6.4 CDS 133836 134360 133836 ID=cds.MS.gene74447.t1;Parent=MS.gene74447.t1
Gene Sequence

>MS.gene74447

ATGTCCAGAAACTCTAGTCTAAGATTATCAGGCAAAATAGCCATCATCACGGGTGGTGCTAGTGGCATCGGTGAAGCCACGGCGCGTGTCTTTGCTAACGAAGGCGTCGGCCATGTTGTGATTGCTGACATCCAAGATGAATTTGGCATTCAAGTAGCTGCGTCCATCGGCAACCAAAGGTGCACCTACATCCACTGTGATGTGGCAGACGAAGATCAAGTCAAAAATCTCGTTCAATCAACGGTTAATACTTATGGACAGGTGCAAACATTTCCTATCTTAGCTTTCAAGGGTGTCTAACTTTTTCTATTTTCAAACATTTTTGCATCATTATGAAAAAATAAATACTATTAAGAGAAATGAGATTATGTTATTCTCACACCAGGTAGATATAATGTTCACCAATGCCGGAATCTTTAGTCCAACCGATCAAACTGTGTTGGAACTCGACATGTCCAACCTAGACCGTTTATTCACAATCAATGTTCGAGGAATGGCATTGTGTGTGAAACATGCAGCCCGTGCAATGGTGGAGGGGCGCGTTAGGGGGAGTATTGTGTGCACGGGGAGTGTTCACGGTAGTCATGGATTCTTGAGGTCAACGGATTACACGATGTCAAAGCACGCAGTATTGGGGCTAATGCGTGCAGCCAGCGTGCAACTGGCCGCACACGGAATAAGAGTGAACTGTGTCTCGCCGAATGGACTCGCGACACCTTTGACTTGTAAATTTTTAGGGGTGAGTGAGGAGAAGGCACAAGAGACTTATAAAGGATATGCAAGACTTGAAGGAGTGGTGCTCACACCTAAGCATGTGGCAGATGCTGTGTTATTTTTAGCTTCGAATGATGCTGAGTTTGTCACTGGCCTTGATCTTAGTGTGGACGGTGGATTTGTTTATGGAAAATAG
Protein sequence

>MS.gene74447.t1

MSRNSSLRLSGKIAIITGGASGIGEATARVFANEGVGHVVIADIQDEFGIQVAASIGNQRCTYIHCDVADEDQVKNLVQSTVNTYGQVDIMFTNAGIFSPTDQTVLELDMSNLDRLFTINVRGMALCVKHAARAMVEGRVRGSIVCTGSVHGSHGFLRSTDYTMSKHAVLGLMRAASVQLAAHGIRVNCVSPNGLATPLTCKFLGVSEEKAQETYKGYARLEGVVLTPKHVADAVLFLASNDAEFVTGLDLSVDGGFVYGK