Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74623.t1 | KEH25024.1 | 79.1 | 177 | 23 | 1 | 72 | 248 | 50 | 212 | 1.30E-74 | 289.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74623.t1 | A0A072U5J3 | 79.1 | 177 | 23 | 1 | 72 | 248 | 50 | 212 | 9.6e-75 | 289.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050750 | MS.gene74623 | 0.807255 | 5.53E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene74623 | 0.812711 | 3.69E-51 | -1.69E-46 |
| MS.gene054405 | MS.gene74623 | 0.801865 | 7.36E-49 | -1.69E-46 |
| MS.gene054657 | MS.gene74623 | 0.800752 | 1.24E-48 | -1.69E-46 |
| MS.gene056498 | MS.gene74623 | 0.801343 | 9.42E-49 | -1.69E-46 |
| MS.gene058548 | MS.gene74623 | 0.800669 | 1.29E-48 | -1.69E-46 |
| MS.gene060634 | MS.gene74623 | -0.808517 | 2.98E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74623.t1 | MTR_6g013220 | 79.070 | 172 | 22 | 1 | 77 | 248 | 55 | 212 | 2.65e-96 | 281 |
| MS.gene74623.t1 | MTR_6g048070 | 44.554 | 101 | 38 | 2 | 1 | 85 | 1 | 99 | 4.42e-18 | 77.8 |
| MS.gene74623.t1 | MTR_6g016175 | 26.295 | 251 | 158 | 8 | 1 | 243 | 1 | 232 | 1.61e-15 | 75.9 |
| MS.gene74623.t1 | MTR_3g448380 | 30.625 | 160 | 107 | 3 | 59 | 214 | 117 | 276 | 2.31e-14 | 71.2 |
| MS.gene74623.t1 | MTR_1g040450 | 30.409 | 171 | 113 | 4 | 76 | 243 | 111 | 278 | 7.24e-14 | 70.5 |
| MS.gene74623.t1 | MTR_7g108760 | 25.926 | 189 | 133 | 4 | 59 | 243 | 357 | 542 | 1.87e-13 | 69.7 |
| MS.gene74623.t1 | MTR_6g048840 | 28.000 | 175 | 112 | 5 | 76 | 243 | 225 | 392 | 1.99e-13 | 69.7 |
| MS.gene74623.t1 | MTR_8g058470 | 25.926 | 189 | 133 | 4 | 59 | 243 | 355 | 540 | 1.99e-13 | 69.7 |
| MS.gene74623.t1 | MTR_2g105880 | 23.950 | 238 | 166 | 5 | 10 | 243 | 67 | 293 | 1.48e-12 | 66.6 |
| MS.gene74623.t1 | MTR_3g022950 | 25.258 | 194 | 135 | 4 | 3 | 193 | 636 | 822 | 5.27e-12 | 65.9 |
| MS.gene74623.t1 | MTR_1g054775 | 29.885 | 174 | 104 | 5 | 76 | 238 | 618 | 784 | 7.77e-12 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAAGAGAGATTTGATTTA+TGG | 0.116291 | 6.3:+22306534 | MS.gene74623:CDS |
| TCGTCATTGGAACATGTTTC+TGG | 0.158342 | 6.3:-22306356 | None:intergenic |
| AAATCGCCAAACATCATTAA+AGG | 0.271490 | 6.3:-22306593 | None:intergenic |
| TCTTGACGTGTCTTTCCTAT+TGG | 0.326012 | 6.3:+22306493 | MS.gene74623:CDS |
| TCAACACCTTTAATGATGTT+TGG | 0.344659 | 6.3:+22306587 | MS.gene74623:CDS |
| TACACCTATGAGCAACCTTA+TGG | 0.346601 | 6.3:-22306229 | None:intergenic |
| TCATTACCCTTTAGTTCTTC+CGG | 0.360197 | 6.3:-22306632 | None:intergenic |
| AACAAATAAGAGATACATCT+TGG | 0.380955 | 6.3:+22306445 | MS.gene74623:CDS |
| TTGACCAAGCCGAACACAAT+TGG | 0.392045 | 6.3:-22306254 | None:intergenic |
| CGCCCTCTATTAGTTCAATC+TGG | 0.398255 | 6.3:+22306863 | MS.gene74623:CDS |
| CCTTGTGGAGCTTCCGTTGT+TGG | 0.424252 | 6.3:+22306706 | MS.gene74623:CDS |
| GACAGGGATTGGTATTCTCT+TGG | 0.442717 | 6.3:+22306390 | MS.gene74623:CDS |
| GAACCAGATTGAACTAATAG+AGG | 0.449982 | 6.3:-22306866 | None:intergenic |
| GTAGGAGGGATTACTGAGTT+AGG | 0.459744 | 6.3:+22306191 | MS.gene74623:CDS |
| TTTGGAACCGGAAGAACTAA+AGG | 0.463362 | 6.3:+22306625 | MS.gene74623:CDS |
| AACTCGGTGCATGAACCAAT+AGG | 0.486014 | 6.3:-22306508 | None:intergenic |
| AGGTGTAATCCAATTGTGTT+CGG | 0.490342 | 6.3:+22306245 | MS.gene74623:CDS |
| AGGAGGAGAACAATGTAAGC+TGG | 0.493023 | 6.3:+22306324 | MS.gene74623:CDS |
| TTGGAACCGGAAGAACTAAA+GGG | 0.493607 | 6.3:+22306626 | MS.gene74623:CDS |
| AAGAGATACATCTTGGTTGA+AGG | 0.497593 | 6.3:+22306452 | MS.gene74623:CDS |
| GAAGGAAAATTGATTCAAGA+AGG | 0.514667 | 6.3:+22306470 | MS.gene74623:CDS |
| GGAATGGCTAGAGCATTTCA+AGG | 0.514887 | 6.3:+22306799 | MS.gene74623:CDS |
| CTCTATCTATTCTGCCTGCA+CGG | 0.531142 | 6.3:-22306763 | None:intergenic |
| TGATCCATAAGGTTGCTCAT+AGG | 0.532353 | 6.3:+22306225 | MS.gene74623:CDS |
| TAATCCAATTGTGTTCGGCT+TGG | 0.532647 | 6.3:+22306250 | MS.gene74623:CDS |
| GACTATGACAATAACCGTGC+AGG | 0.543680 | 6.3:+22306749 | MS.gene74623:CDS |
| TGTTCCAATGACGAGAAAAG+TGG | 0.545436 | 6.3:+22306365 | MS.gene74623:CDS |
| GTTCCAATGACGAGAAAAGT+GGG | 0.548017 | 6.3:+22306366 | MS.gene74623:CDS |
| AAAATCTGCGATGATCCATA+AGG | 0.558872 | 6.3:+22306214 | MS.gene74623:CDS |
| GATGACATTACAAGCGTATG+TGG | 0.559882 | 6.3:+22306893 | MS.gene74623:CDS |
| GGTAATGAGGTGACTTCACA+AGG | 0.574743 | 6.3:+22306647 | MS.gene74623:CDS |
| GGAGGAGAACAATGTAAGCT+GGG | 0.576028 | 6.3:+22306325 | MS.gene74623:CDS |
| AGTTAAAAGATGGTGGAAGG+AGG | 0.580076 | 6.3:+22306307 | MS.gene74623:CDS |
| TCATTTCAGCTATACAATGT+CGG | 0.588108 | 6.3:-22306682 | None:intergenic |
| AAAAGTTAAAAGATGGTGGA+AGG | 0.594675 | 6.3:+22306304 | MS.gene74623:CDS |
| AATCTCTCTTGACTGCAACT+CGG | 0.607370 | 6.3:-22306524 | None:intergenic |
| GGAAGAACTAAAGGGTAATG+AGG | 0.610801 | 6.3:+22306634 | MS.gene74623:CDS |
| AACCAGATTGAACTAATAGA+GGG | 0.638427 | 6.3:-22306865 | None:intergenic |
| GTGAACTTTCTGTCCAACAA+CGG | 0.668255 | 6.3:-22306719 | None:intergenic |
| TCCAATGACGAGAAAAGTGG+GGG | 0.678217 | 6.3:+22306368 | MS.gene74623:CDS |
| TTCCAATGACGAGAAAAGTG+GGG | 0.679735 | 6.3:+22306367 | MS.gene74623:CDS |
| TGTTGGACAGAAAGTTCACA+TGG | 0.688863 | 6.3:+22306723 | MS.gene74623:CDS |
| CCAACAACGGAAGCTCCACA+AGG | 0.688911 | 6.3:-22306706 | None:intergenic |
| TATAGCTGAAATGAACCTTG+TGG | 0.699115 | 6.3:+22306691 | MS.gene74623:CDS |
| CAGATTGAACTAATAGAGGG+CGG | 0.706620 | 6.3:-22306862 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAAATAAGAGATACATCT+TGG | + | chr6.3:22306445-22306464 | MS.gene74623:CDS | 25.0% |
| ! | ATGAAAAAGTTAAAAGATGG+TGG | + | chr6.3:22306300-22306319 | MS.gene74623:CDS | 25.0% |
| !!! | CCATCTTTTAACTTTTTCAT+CGG | - | chr6.3:22306300-22306319 | None:intergenic | 25.0% |
| AAATCGCCAAACATCATTAA+AGG | - | chr6.3:22306596-22306615 | None:intergenic | 30.0% | |
| AACCAGATTGAACTAATAGA+GGG | - | chr6.3:22306868-22306887 | None:intergenic | 30.0% | |
| CCGATGAAAAAGTTAAAAGA+TGG | + | chr6.3:22306297-22306316 | MS.gene74623:CDS | 30.0% | |
| TCAACACCTTTAATGATGTT+TGG | + | chr6.3:22306587-22306606 | MS.gene74623:CDS | 30.0% | |
| TCATTTCAGCTATACAATGT+CGG | - | chr6.3:22306685-22306704 | None:intergenic | 30.0% | |
| ! | AGATAGAGTTTTTGTAGATC+AGG | + | chr6.3:22306778-22306797 | MS.gene74623:CDS | 30.0% |
| ! | GAAGGAAAATTGATTCAAGA+AGG | + | chr6.3:22306470-22306489 | MS.gene74623:CDS | 30.0% |
| ! | GTCAAGAGAGATTTGATTTA+TGG | + | chr6.3:22306534-22306553 | MS.gene74623:CDS | 30.0% |
| !! | AAAAGTTAAAAGATGGTGGA+AGG | + | chr6.3:22306304-22306323 | MS.gene74623:CDS | 30.0% |
| !!! | TTTTCAAGAAGTTTTGGAAC+CGG | + | chr6.3:22306613-22306632 | MS.gene74623:CDS | 30.0% |
| AAAATCTGCGATGATCCATA+AGG | + | chr6.3:22306214-22306233 | MS.gene74623:CDS | 35.0% | |
| AAGAGATACATCTTGGTTGA+AGG | + | chr6.3:22306452-22306471 | MS.gene74623:CDS | 35.0% | |
| AGGTGTAATCCAATTGTGTT+CGG | + | chr6.3:22306245-22306264 | MS.gene74623:CDS | 35.0% | |
| GAACCAGATTGAACTAATAG+AGG | - | chr6.3:22306869-22306888 | None:intergenic | 35.0% | |
| TATAGCTGAAATGAACCTTG+TGG | + | chr6.3:22306691-22306710 | MS.gene74623:CDS | 35.0% | |
| ! | GAGTTTTTGTAGATCAGGAA+TGG | + | chr6.3:22306783-22306802 | MS.gene74623:CDS | 35.0% |
| !! | TCATTACCCTTTAGTTCTTC+CGG | - | chr6.3:22306635-22306654 | None:intergenic | 35.0% |
| !! | TGGCGATTTTCAAGAAGTTT+TGG | + | chr6.3:22306607-22306626 | MS.gene74623:CDS | 35.0% |
| !!! | AGGATTTTAGGTTGGAATGT+AGG | + | chr6.3:22306173-22306192 | MS.gene74623:CDS | 35.0% |
| !!! | ATTTCAAGGTTTTGAAGCTG+CGG | + | chr6.3:22306813-22306832 | MS.gene74623:CDS | 35.0% |
| !!! | ATTTTAGGTTGGAATGTAGG+AGG | + | chr6.3:22306176-22306195 | MS.gene74623:CDS | 35.0% |
| !!! | TTTTAGGTTGGAATGTAGGA+GGG | + | chr6.3:22306177-22306196 | MS.gene74623:CDS | 35.0% |
| AATCTCTCTTGACTGCAACT+CGG | - | chr6.3:22306527-22306546 | None:intergenic | 40.0% | |
| CAGATTGAACTAATAGAGGG+CGG | - | chr6.3:22306865-22306884 | None:intergenic | 40.0% | |
| GATGACATTACAAGCGTATG+TGG | + | chr6.3:22306893-22306912 | MS.gene74623:CDS | 40.0% | |
| GGAAGAACTAAAGGGTAATG+AGG | + | chr6.3:22306634-22306653 | MS.gene74623:CDS | 40.0% | |
| GTGAACTTTCTGTCCAACAA+CGG | - | chr6.3:22306722-22306741 | None:intergenic | 40.0% | |
| GTTCCAATGACGAGAAAAGT+GGG | + | chr6.3:22306366-22306385 | MS.gene74623:CDS | 40.0% | |
| TAATCCAATTGTGTTCGGCT+TGG | + | chr6.3:22306250-22306269 | MS.gene74623:CDS | 40.0% | |
| TACACCTATGAGCAACCTTA+TGG | - | chr6.3:22306232-22306251 | None:intergenic | 40.0% | |
| TCGTCATTGGAACATGTTTC+TGG | - | chr6.3:22306359-22306378 | None:intergenic | 40.0% | |
| TCTTGACGTGTCTTTCCTAT+TGG | + | chr6.3:22306493-22306512 | MS.gene74623:CDS | 40.0% | |
| TGATCCATAAGGTTGCTCAT+AGG | + | chr6.3:22306225-22306244 | MS.gene74623:CDS | 40.0% | |
| TGTTCCAATGACGAGAAAAG+TGG | + | chr6.3:22306365-22306384 | MS.gene74623:CDS | 40.0% | |
| TGTTGGACAGAAAGTTCACA+TGG | + | chr6.3:22306723-22306742 | MS.gene74623:CDS | 40.0% | |
| TTCCAATGACGAGAAAAGTG+GGG | + | chr6.3:22306367-22306386 | MS.gene74623:CDS | 40.0% | |
| TTGGAACCGGAAGAACTAAA+GGG | + | chr6.3:22306626-22306645 | MS.gene74623:CDS | 40.0% | |
| ! | TTTGGAACCGGAAGAACTAA+AGG | + | chr6.3:22306625-22306644 | MS.gene74623:CDS | 40.0% |
| !! | AGTTAAAAGATGGTGGAAGG+AGG | + | chr6.3:22306307-22306326 | MS.gene74623:CDS | 40.0% |
| !!! | TTTCAAGGTTTTGAAGCTGC+GGG | + | chr6.3:22306814-22306833 | MS.gene74623:CDS | 40.0% |
| AGGAGGAGAACAATGTAAGC+TGG | + | chr6.3:22306324-22306343 | MS.gene74623:CDS | 45.0% | |
| CGCCCTCTATTAGTTCAATC+TGG | + | chr6.3:22306863-22306882 | MS.gene74623:CDS | 45.0% | |
| CTCTATCTATTCTGCCTGCA+CGG | - | chr6.3:22306766-22306785 | None:intergenic | 45.0% | |
| GACTATGACAATAACCGTGC+AGG | + | chr6.3:22306749-22306768 | MS.gene74623:CDS | 45.0% | |
| GGAGGAGAACAATGTAAGCT+GGG | + | chr6.3:22306325-22306344 | MS.gene74623:CDS | 45.0% | |
| GGTAATGAGGTGACTTCACA+AGG | + | chr6.3:22306647-22306666 | MS.gene74623:CDS | 45.0% | |
| GTAGGAGGGATTACTGAGTT+AGG | + | chr6.3:22306191-22306210 | MS.gene74623:CDS | 45.0% | |
| TCCAATGACGAGAAAAGTGG+GGG | + | chr6.3:22306368-22306387 | MS.gene74623:CDS | 45.0% | |
| TTGACCAAGCCGAACACAAT+TGG | - | chr6.3:22306257-22306276 | None:intergenic | 45.0% | |
| ! | GACAGGGATTGGTATTCTCT+TGG | + | chr6.3:22306390-22306409 | MS.gene74623:CDS | 45.0% |
| ! | GGAATGGCTAGAGCATTTCA+AGG | + | chr6.3:22306799-22306818 | MS.gene74623:CDS | 45.0% |
| !! | AACTCGGTGCATGAACCAAT+AGG | - | chr6.3:22306511-22306530 | None:intergenic | 45.0% |
| !!! | TTCAAGGTTTTGAAGCTGCG+GGG | + | chr6.3:22306815-22306834 | MS.gene74623:CDS | 45.0% |
| !! | TCCCCCACTTTTCTCGTCAT+TGG | - | chr6.3:22306372-22306391 | None:intergenic | 50.0% |
| CCAACAACGGAAGCTCCACA+AGG | - | chr6.3:22306709-22306728 | None:intergenic | 55.0% | |
| CCTTGTGGAGCTTCCGTTGT+TGG | + | chr6.3:22306706-22306725 | MS.gene74623:CDS | 55.0% | |
| GAAAAGTGGGGGACAGGGAT+TGG | + | chr6.3:22306379-22306398 | MS.gene74623:CDS | 55.0% | |
| GACGAGAAAAGTGGGGGACA+GGG | + | chr6.3:22306374-22306393 | MS.gene74623:CDS | 55.0% | |
| TGACGAGAAAAGTGGGGGAC+AGG | + | chr6.3:22306373-22306392 | MS.gene74623:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 22306170 | 22306922 | 22306170 | ID=MS.gene74623 |
| chr6.3 | mRNA | 22306170 | 22306922 | 22306170 | ID=MS.gene74623.t1;Parent=MS.gene74623 |
| chr6.3 | exon | 22306170 | 22306922 | 22306170 | ID=MS.gene74623.t1.exon1;Parent=MS.gene74623.t1 |
| chr6.3 | CDS | 22306170 | 22306922 | 22306170 | ID=cds.MS.gene74623.t1;Parent=MS.gene74623.t1 |
| Gene Sequence |
| Protein sequence |
