Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74641.t1 | XP_013454168.1 | 87 | 46 | 6 | 0 | 32 | 77 | 1597 | 1642 | 8.50E-12 | 79.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74641.t1 | Q9FGI1 | 44.4 | 72 | 40 | 0 | 7 | 78 | 1567 | 1638 | 9.9e-09 | 60.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74641.t1 | G7KFI6 | 87.0 | 46 | 6 | 0 | 32 | 77 | 1402 | 1447 | 6.2e-12 | 79.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene74641 | 0.849274 | 3.59E-60 | -1.69E-46 |
| MS.gene05329 | MS.gene74641 | 0.806404 | 8.36E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene74641 | 0.805293 | 1.43E-49 | -1.69E-46 |
| MS.gene059458 | MS.gene74641 | 0.811484 | 6.84E-51 | -1.69E-46 |
| MS.gene059460 | MS.gene74641 | 0.80454 | 2.06E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74641.t1 | MTR_5g074510 | 68.852 | 61 | 19 | 0 | 14 | 74 | 1384 | 1444 | 1.64e-20 | 84.0 |
| MS.gene74641.t1 | MTR_5g074510 | 68.852 | 61 | 19 | 0 | 14 | 74 | 1579 | 1639 | 1.64e-20 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74641.t1 | AT5G58410 | 46.970 | 66 | 35 | 0 | 7 | 72 | 1567 | 1632 | 1.36e-13 | 64.3 |
| MS.gene74641.t1 | AT5G58410 | 46.970 | 66 | 35 | 0 | 7 | 72 | 1554 | 1619 | 1.40e-13 | 64.3 |
Find 13 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAAGATGCAGAGCTTTC+TGG | 0.220031 | 6.3:+22542202 | MS.gene74641:CDS |
| CTACAGGAATATGTTGAAAA+AGG | 0.283729 | 6.3:-22542153 | None:intergenic |
| CAAACCTCAATCTTTCTAAC+TGG | 0.286509 | 6.3:-22541502 | None:intergenic |
| CTTCTTCAGACTCTGATTCA+AGG | 0.407382 | 6.3:-22542182 | None:intergenic |
| TTGCTAGATAGAACATCATC+AGG | 0.436859 | 6.3:+22541437 | MS.gene74641:CDS |
| CTCCCCAGTTAGAAAGATTG+AGG | 0.453096 | 6.3:+22541498 | MS.gene74641:CDS |
| TAGCAGAATCTTGAATGTAC+TGG | 0.456123 | 6.3:-22542111 | None:intergenic |
| AGGTGAGACATTTACAAAGA+TGG | 0.461688 | 6.3:-22542689 | None:intergenic |
| TTCTTCAGACTCTGATTCAA+GGG | 0.489664 | 6.3:-22542181 | None:intergenic |
| TGATTCAAGGGAATCTCTAC+AGG | 0.493305 | 6.3:-22542169 | None:intergenic |
| AAACCTCAATCTTTCTAACT+GGG | 0.546425 | 6.3:-22541501 | None:intergenic |
| AACCTCAATCTTTCTAACTG+GGG | 0.664072 | 6.3:-22541500 | None:intergenic |
| TGCTAGATAGAACATCATCA+GGG | 0.676015 | 6.3:+22541438 | MS.gene74641:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATTGACATATTTATA+GGG | - | chr6.3:22540877-22540896 | None:intergenic | 10.0% |
| !! | TAAAATTTATTTGTAACAAA+CGG | + | chr6.3:22541345-22541364 | MS.gene74641:intron | 10.0% |
| !!! | TTATTAGTGTATTTTATTTT+GGG | + | chr6.3:22541756-22541775 | MS.gene74641:intron | 10.0% |
| !!! | TTTATTAGTGTATTTTATTT+TGG | + | chr6.3:22541755-22541774 | MS.gene74641:intron | 10.0% |
| !! | AAAATTTATTTGTAACAAAC+GGG | + | chr6.3:22541346-22541365 | MS.gene74641:intron | 15.0% |
| !! | ATAAACATAATATGTGTAAT+GGG | + | chr6.3:22541553-22541572 | MS.gene74641:intron | 15.0% |
| !! | CAAAAAATTGACATATTTAT+AGG | - | chr6.3:22540878-22540897 | None:intergenic | 15.0% |
| !!! | ATAATTGCAATATTTTATAG+AGG | + | chr6.3:22541984-22542003 | MS.gene74641:intron | 15.0% |
| !!! | TAATTGCAATATTTTATAGA+GGG | + | chr6.3:22541985-22542004 | MS.gene74641:intron | 15.0% |
| !!! | TATTTAACCCTATTTTTAAA+TGG | - | chr6.3:22540844-22540863 | None:intergenic | 15.0% |
| !! | ATTTGAGCCATTTAAAAATA+GGG | + | chr6.3:22540834-22540853 | MS.gene74641:intron | 20.0% |
| !! | CATAAACATAATATGTGTAA+TGG | + | chr6.3:22541552-22541571 | MS.gene74641:intron | 20.0% |
| !!! | AAATGAGTATTTTACAAACA+AGG | + | chr6.3:22541213-22541232 | MS.gene74641:intron | 20.0% |
| !!! | AATTGCAATATTTTATAGAG+GGG | + | chr6.3:22541986-22542005 | MS.gene74641:intron | 20.0% |
| !!! | TTTTTTTTTTTTCCTTTGCA+GGG | + | chr6.3:22542663-22542682 | MS.gene74641:intron | 20.0% |
| ! | AAACTAAAGCATACATTGAT+TGG | - | chr6.3:22541738-22541757 | None:intergenic | 25.0% |
| ! | ATATAAACTTGAAGTGAGAT+GGG | + | chr6.3:22541175-22541194 | MS.gene74641:intron | 25.0% |
| ! | ATATGACTTAGACAAATGAA+AGG | - | chr6.3:22542633-22542652 | None:intergenic | 25.0% |
| ! | CAAAAAATTTCACAAACACA+CGG | - | chr6.3:22541923-22541942 | None:intergenic | 25.0% |
| ! | CACATATGAGTTTACTTTAA+AGG | - | chr6.3:22540972-22540991 | None:intergenic | 25.0% |
| ! | CATTTGAGCCATTTAAAAAT+AGG | + | chr6.3:22540833-22540852 | MS.gene74641:intron | 25.0% |
| ! | GCACAAAAAAATTAACGAAA+AGG | - | chr6.3:22541593-22541612 | None:intergenic | 25.0% |
| ! | GTAATGTTTCATACATTTGA+AGG | - | chr6.3:22541615-22541634 | None:intergenic | 25.0% |
| !! | ATATGAGTTTACTTTAAAGG+AGG | - | chr6.3:22540969-22540988 | None:intergenic | 25.0% |
| !! | ATTTTGTTGCTGATATTTTG+AGG | + | chr6.3:22541789-22541808 | MS.gene74641:intron | 25.0% |
| !! | GTAATTTTATCTGACTCAAT+AGG | - | chr6.3:22542306-22542325 | None:intergenic | 25.0% |
| !! | TAGGGTTAAAATTAGCTTAT+GGG | - | chr6.3:22541303-22541322 | None:intergenic | 25.0% |
| !! | TAGGGTTTAGGTTAAAATTA+GGG | - | chr6.3:22541321-22541340 | None:intergenic | 25.0% |
| !! | TATGAGTTTACTTTAAAGGA+GGG | - | chr6.3:22540968-22540987 | None:intergenic | 25.0% |
| !! | TTAGGGTTAAAATTAGCTTA+TGG | - | chr6.3:22541304-22541323 | None:intergenic | 25.0% |
| !!! | ACAAATAAATTTTAGCCCTA+GGG | - | chr6.3:22541339-22541358 | None:intergenic | 25.0% |
| !!! | ATTTTGGTTGATTTAATGAC+AGG | + | chr6.3:22541104-22541123 | MS.gene74641:intron | 25.0% |
| !!! | CTTTAAAGTAAACTCATATG+TGG | + | chr6.3:22540970-22540989 | MS.gene74641:intron | 25.0% |
| !!! | CTTTTTTGAATATTTGAGCT+TGG | + | chr6.3:22541874-22541893 | MS.gene74641:intron | 25.0% |
| !!! | GTTTTTTTTTTTTCCTTTGC+AGG | + | chr6.3:22542662-22542681 | MS.gene74641:intron | 25.0% |
| !!! | TAATTTTAACCTAAACCCTA+GGG | + | chr6.3:22541321-22541340 | MS.gene74641:intron | 25.0% |
| !!! | TACAAATAAATTTTAGCCCT+AGG | - | chr6.3:22541340-22541359 | None:intergenic | 25.0% |
| AAACAAAACTGCACTTTATG+TGG | - | chr6.3:22542524-22542543 | None:intergenic | 30.0% | |
| AAACCTCAATCTTTCTAACT+GGG | - | chr6.3:22541504-22541523 | None:intergenic | 30.0% | |
| AAGTGAAGTTGGAAAACATT+TGG | - | chr6.3:22540940-22540959 | None:intergenic | 30.0% | |
| AGTGAAGTTGGAAAACATTT+GGG | - | chr6.3:22540939-22540958 | None:intergenic | 30.0% | |
| CGGTACATATTTAGTTATCT+TGG | + | chr6.3:22542586-22542605 | MS.gene74641:intron | 30.0% | |
| CTACAGGAATATGTTGAAAA+AGG | - | chr6.3:22542156-22542175 | None:intergenic | 30.0% | |
| CTGCAGAAAAATTTCATGTT+GGG | - | chr6.3:22541412-22541431 | None:intergenic | 30.0% | |
| GATAAAATTACATCACTTGC+TGG | + | chr6.3:22542315-22542334 | MS.gene74641:intron | 30.0% | |
| GATATAAACTTGAAGTGAGA+TGG | + | chr6.3:22541174-22541193 | MS.gene74641:intron | 30.0% | |
| GTCTAAGTCATATACTCAAA+TGG | + | chr6.3:22542640-22542659 | MS.gene74641:intron | 30.0% | |
| TTGTGCAACTTAACAAAATC+AGG | - | chr6.3:22542560-22542579 | None:intergenic | 30.0% | |
| TTTAAATGGCTCAAATGCAA+AGG | - | chr6.3:22540830-22540849 | None:intergenic | 30.0% | |
| ! | CTAGGGTTTAGGTTAAAATT+AGG | - | chr6.3:22541322-22541341 | None:intergenic | 30.0% |
| ! | GTTAAGTTGCACAATATACT+CGG | + | chr6.3:22542566-22542585 | MS.gene74641:intron | 30.0% |
| ! | TGCAAAAGTAACAGTTTTCA+TGG | + | chr6.3:22541833-22541852 | MS.gene74641:intron | 30.0% |
| ! | TTTTGAATATTTGAGCTTGG+TGG | + | chr6.3:22541877-22541896 | MS.gene74641:intron | 30.0% |
| !! | CTAATTTTAACCTAAACCCT+AGG | + | chr6.3:22541320-22541339 | MS.gene74641:intron | 30.0% |
| AACACATCAAGCTCTCTAAT+TGG | - | chr6.3:22541641-22541660 | None:intergenic | 35.0% | |
| AACCTCAATCTTTCTAACTG+GGG | - | chr6.3:22541503-22541522 | None:intergenic | 35.0% | |
| ACACATCAAGCTCTCTAATT+GGG | - | chr6.3:22541640-22541659 | None:intergenic | 35.0% | |
| ACATGAAATTACACCATACC+TGG | - | chr6.3:22542501-22542520 | None:intergenic | 35.0% | |
| CAAACCTCAATCTTTCTAAC+TGG | - | chr6.3:22541505-22541524 | None:intergenic | 35.0% | |
| CATGAAATTACACCATACCT+GGG | - | chr6.3:22542500-22542519 | None:intergenic | 35.0% | |
| CCTGCAGAAAAATTTCATGT+TGG | - | chr6.3:22541413-22541432 | None:intergenic | 35.0% | |
| GTGAAGTTGGAAAACATTTG+GGG | - | chr6.3:22540938-22540957 | None:intergenic | 35.0% | |
| GTGTTCATCATCATCTGATA+GGG | + | chr6.3:22542078-22542097 | MS.gene74641:intron | 35.0% | |
| TAGCAGAATCTTGAATGTAC+TGG | - | chr6.3:22542114-22542133 | None:intergenic | 35.0% | |
| TGAAGTTGGAAAACATTTGG+GGG | - | chr6.3:22540937-22540956 | None:intergenic | 35.0% | |
| TGCTAGATAGAACATCATCA+GGG | + | chr6.3:22541438-22541457 | MS.gene74641:CDS | 35.0% | |
| TGTGTTCATCATCATCTGAT+AGG | + | chr6.3:22542077-22542096 | MS.gene74641:intron | 35.0% | |
| TTCTTCAGACTCTGATTCAA+GGG | - | chr6.3:22542184-22542203 | None:intergenic | 35.0% | |
| TTGCTAGATAGAACATCATC+AGG | + | chr6.3:22541437-22541456 | MS.gene74641:CDS | 35.0% | |
| TTTCTTTCTTCAGATGACAG+TGG | - | chr6.3:22542041-22542060 | None:intergenic | 35.0% | |
| TTTGTAACAAACGGGATCTT+AGG | + | chr6.3:22541354-22541373 | MS.gene74641:intron | 35.0% | |
| ! | AGTAACAGTTTTCATGGTTG+TGG | + | chr6.3:22541839-22541858 | MS.gene74641:intron | 35.0% |
| ! | CCAACATGAAATTTTTCTGC+AGG | + | chr6.3:22541410-22541429 | MS.gene74641:intron | 35.0% |
| !! | AAATTTTAGCCCTAGGGTTT+AGG | - | chr6.3:22541333-22541352 | None:intergenic | 35.0% |
| !! | ATTTTGAGGATGATGGTTGT+GGG | + | chr6.3:22541803-22541822 | MS.gene74641:intron | 35.0% |
| !! | TATTTTGAGGATGATGGTTG+TGG | + | chr6.3:22541802-22541821 | MS.gene74641:intron | 35.0% |
| !! | TGCTGATATTTTGAGGATGA+TGG | + | chr6.3:22541796-22541815 | MS.gene74641:intron | 35.0% |
| AAGAAAGATGCAGAGCTTTC+TGG | + | chr6.3:22542202-22542221 | MS.gene74641:CDS | 40.0% | |
| ATGGGAGTTCTCTAGTATTG+AGG | + | chr6.3:22540795-22540814 | MS.gene74641:intron | 40.0% | |
| ATTCATCTGCAATGAGAGGA+TGG | - | chr6.3:22542474-22542493 | None:intergenic | 40.0% | |
| CACATCAAGCTCTCTAATTG+GGG | - | chr6.3:22541639-22541658 | None:intergenic | 40.0% | |
| CTGTCATTGAATCCTTTACC+AGG | + | chr6.3:22542437-22542456 | MS.gene74641:intron | 40.0% | |
| CTTCTTCAGACTCTGATTCA+AGG | - | chr6.3:22542185-22542204 | None:intergenic | 40.0% | |
| GACAATTCATCTGCAATGAG+AGG | - | chr6.3:22542478-22542497 | None:intergenic | 40.0% | |
| GTTATTCCTGCTTATGTTCG+TGG | + | chr6.3:22542363-22542382 | MS.gene74641:intron | 40.0% | |
| TAGAGAGCTTGATGTGTTAG+CGG | + | chr6.3:22541643-22541662 | MS.gene74641:intron | 40.0% | |
| TGATTCAAGGGAATCTCTAC+AGG | - | chr6.3:22542172-22542191 | None:intergenic | 40.0% | |
| TGTTCTCTGTGGAATCTCTT+TGG | + | chr6.3:22542280-22542299 | MS.gene74641:intron | 40.0% | |
| TTTGGGGGACTAAAAGTTGA+AGG | - | chr6.3:22540922-22540941 | None:intergenic | 40.0% | |
| ! | TCACTTGCTGGAGCAATATA+TGG | + | chr6.3:22542327-22542346 | MS.gene74641:intron | 40.0% |
| !! | TAGAGGGGTGCTTTCTATTA+AGG | + | chr6.3:22542001-22542020 | MS.gene74641:intron | 40.0% |
| AGCGACACAGTTGATTGATG+AGG | + | chr6.3:22541703-22541722 | MS.gene74641:intron | 45.0% | |
| AGGCAGATAATTGTCGTTGC+GGG | + | chr6.3:22541124-22541143 | MS.gene74641:intron | 45.0% | |
| CAAAGATGGTTTCCCTGCAA+AGG | - | chr6.3:22542678-22542697 | None:intergenic | 45.0% | |
| CAGATGAATTGTCCCAGGTA+TGG | + | chr6.3:22542485-22542504 | MS.gene74641:intron | 45.0% | |
| CAGGCAGATAATTGTCGTTG+CGG | + | chr6.3:22541123-22541142 | MS.gene74641:intron | 45.0% | |
| CATTGCAGATGAATTGTCCC+AGG | + | chr6.3:22542480-22542499 | MS.gene74641:intron | 45.0% | |
| CTCCCCAGTTAGAAAGATTG+AGG | + | chr6.3:22541498-22541517 | MS.gene74641:CDS | 45.0% | |
| GAACAACAGTGAACCAGTGT+TGG | - | chr6.3:22542265-22542284 | None:intergenic | 45.0% | |
| GGAGGGACAAAAAGTGAAGT+TGG | - | chr6.3:22540951-22540970 | None:intergenic | 45.0% | |
| TGGTTCACTGTTGTTCTCTG+TGG | + | chr6.3:22542269-22542288 | MS.gene74641:intron | 45.0% | |
| ! | AAGATGCAGAGCTTTCTGGT+AGG | + | chr6.3:22542206-22542225 | MS.gene74641:intron | 45.0% |
| ! | TGAGCTTGGTGGATCTGAAA+TGG | + | chr6.3:22541888-22541907 | MS.gene74641:intron | 45.0% |
| !! | ATATATATATAATAAGAAAT+GGG | - | chr6.3:22541239-22541258 | None:intergenic | 5.0% |
| !! | TATATATATATAATAAGAAA+TGG | - | chr6.3:22541240-22541259 | None:intergenic | 5.0% |
| !!! | AAAAAATTCTAAATTTTTTT+GGG | - | chr6.3:22541053-22541072 | None:intergenic | 5.0% |
| !!! | AAATATGAATTTTTATATTT+TGG | + | chr6.3:22541088-22541107 | MS.gene74641:intron | 5.0% |
| !!! | TAAAAAATTCTAAATTTTTT+TGG | - | chr6.3:22541054-22541073 | None:intergenic | 5.0% |
| AAGCAGCCACGAACATAAGC+AGG | - | chr6.3:22542372-22542391 | None:intergenic | 50.0% | |
| GGCAGATAATTGTCGTTGCG+GGG | + | chr6.3:22541125-22541144 | MS.gene74641:intron | 50.0% | |
| TGCGTTTAAGCCACCAACAC+TGG | + | chr6.3:22542249-22542268 | MS.gene74641:intron | 50.0% | |
| TTCGTGGCTGCTTATGTTCG+TGG | + | chr6.3:22542379-22542398 | MS.gene74641:intron | 50.0% | |
| ! | CAACAGTGAACCAGTGTTGG+TGG | - | chr6.3:22542262-22542281 | None:intergenic | 50.0% |
| AATGAGAGGATGGCTGCAGC+AGG | - | chr6.3:22542464-22542483 | None:intergenic | 55.0% | |
| CGTCGATATGCTGACGGAGA+GGG | + | chr6.3:22541666-22541685 | MS.gene74641:intron | 55.0% | |
| TGGCTGCTTATGTTCGTGGC+TGG | + | chr6.3:22542383-22542402 | MS.gene74641:intron | 55.0% | |
| !! | TAGCGGCGTCGATATGCTGA+CGG | + | chr6.3:22541660-22541679 | MS.gene74641:intron | 55.0% |
| GCTGCAGCAGGTCCTGGTAA+AGG | - | chr6.3:22542452-22542471 | None:intergenic | 60.0% | |
| ! | GCGTCGATATGCTGACGGAG+AGG | + | chr6.3:22541665-22541684 | MS.gene74641:intron | 60.0% |
| AGGATGGCTGCAGCAGGTCC+TGG | - | chr6.3:22542458-22542477 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 22540786 | 22542700 | 22540786 | ID=MS.gene74641 |
| chr6.3 | mRNA | 22540786 | 22542700 | 22540786 | ID=MS.gene74641.t1;Parent=MS.gene74641 |
| chr6.3 | exon | 22540786 | 22540792 | 22540786 | ID=MS.gene74641.t1.exon1;Parent=MS.gene74641.t1 |
| chr6.3 | CDS | 22540786 | 22540792 | 22540786 | ID=cds.MS.gene74641.t1;Parent=MS.gene74641.t1 |
| chr6.3 | exon | 22541432 | 22541519 | 22541432 | ID=MS.gene74641.t1.exon2;Parent=MS.gene74641.t1 |
| chr6.3 | CDS | 22541432 | 22541519 | 22541432 | ID=cds.MS.gene74641.t1;Parent=MS.gene74641.t1 |
| chr6.3 | exon | 22542099 | 22542223 | 22542099 | ID=MS.gene74641.t1.exon3;Parent=MS.gene74641.t1 |
| chr6.3 | CDS | 22542099 | 22542223 | 22542099 | ID=cds.MS.gene74641.t1;Parent=MS.gene74641.t1 |
| chr6.3 | exon | 22542684 | 22542700 | 22542684 | ID=MS.gene74641.t1.exon4;Parent=MS.gene74641.t1 |
| chr6.3 | CDS | 22542684 | 22542700 | 22542684 | ID=cds.MS.gene74641.t1;Parent=MS.gene74641.t1 |
| Gene Sequence |
| Protein sequence |
